CN215856041U - Adipose tissue-derived stem cell separation device - Google Patents
Adipose tissue-derived stem cell separation device Download PDFInfo
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- CN215856041U CN215856041U CN202122032302.5U CN202122032302U CN215856041U CN 215856041 U CN215856041 U CN 215856041U CN 202122032302 U CN202122032302 U CN 202122032302U CN 215856041 U CN215856041 U CN 215856041U
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Abstract
The utility model provides a fat mesenchymal stem cell separation device, which relates to the technical field of cell separation, and comprises: the device comprises a tank body, a support column, a support plate, a funnel, a filter plate, filter paper, a connecting pipe, a feed inlet, a valve, a motor, a stirring shaft, blades, a discharge port and an electromagnetic valve; the tank body is a hollow cavity; the four pillars are respectively fixed on the top surface of the tank body; the supporting plate is connected with the top surfaces of the four supporting columns, and through holes are arranged on the supporting plate in a penetrating manner; the funnel is arranged on the top surface of the supporting plate, and at least part of the funnel is embedded into the through hole; the filter plate is provided with at least two filter holes and is embedded in the funnel; the filter paper is placed on the filter plate; one end of the connecting pipe is communicated with the bottom of the funnel; the feed inlet is arranged on the top surface of the tank body, the feed inlet is communicated with the interior of the tank body, and the feed inlet is communicated with the other end of the connecting pipe; one end of the valve is communicated with the feeding hole.
Description
Technical Field
The utility model relates to the technical field of cell sap separation, in particular to a fat mesenchymal stem cell separation device.
Background
Currently, in the related art, cell separation is a device used when separating cells, and in the prior art, the efficiency of separating cells is low. Therefore, it is necessary to design a cell separation device which can improve the cell separation efficiency, ensure the stability of cell fluid and improve the applicability of the device.
SUMMERY OF THE UTILITY MODEL
The present invention is directed to solving at least one of the problems of the prior art or the related art.
Therefore, the utility model provides a fat mesenchymal stem cell separation device.
In view of this, the present invention provides an adipose-derived mesenchymal stem cell isolation apparatus, including: the device comprises a tank body, a support column, a support plate, a funnel, a filter plate, filter paper, a connecting pipe, a feed inlet, a valve, a motor, a stirring shaft, blades, a discharge port and an electromagnetic valve; the tank body is a hollow cavity; the four pillars are respectively fixed on the top surface of the tank body; the supporting plate is connected with the top surfaces of the four supporting columns, and through holes are arranged on the supporting plate in a penetrating manner; the funnel is arranged on the top surface of the supporting plate, and at least part of the funnel is embedded into the through hole; the filter plate is provided with at least two filter holes and is embedded in the funnel; the filter paper is placed on the filter plate; one end of the connecting pipe is communicated with the bottom of the funnel; the feed inlet is arranged on the top surface of the tank body, the feed inlet is communicated with the interior of the tank body, and the feed inlet is communicated with the other end of the connecting pipe; one end of the valve is communicated with the feeding hole; the motor comprises an output shaft, the motor is fixed on the top surface of the tank body, and the output shaft penetrates through the top surface of the tank body; the stirring shaft is connected with the output shaft, the stirring shaft is embedded into the tank body, and the stirring shaft and the output shaft do not rotate relatively; at least two blades are connected with the outer wall of the stirring shaft; the discharge port is arranged at the bottom of the tank body and communicated with the inside of the tank body; the electromagnetic valve is communicated with the discharge port.
In the technical scheme, firstly, the tank body is a hollow cavity, and the four pillars are respectively fixed on the top surface of the tank body, so that the four pillars are connected with the top surface of the tank body; secondly, the supporting plate is connected with the top surfaces of the four pillars, and the supporting plate is provided with through holes in a penetrating manner, so that the supporting plate is connected with the top surfaces of the pillars; thirdly, the funnel is arranged on the top surface of the supporting plate, and at least part of the funnel is embedded into the through hole, so that the function of fixing the position of the funnel is realized; thirdly, the filter plate is provided with at least two filter holes, the filter plate is embedded in the funnel, and the filter paper is placed on the filter plate, so that the cell sap is filtered by the filter paper; thirdly, one end of the connecting pipe is communicated with the bottom of the funnel, so that the effect of communicating the funnel with the connecting pipe is realized; thirdly, the feed port is arranged on the top surface of the tank body, the feed port is communicated with the interior of the tank body, and the feed port is communicated with the other end of the connecting pipe, so that cell sap enters the feed port through the connecting pipe and then enters the interior of the tank body; thirdly, one end of the valve is communicated with the feeding hole, so that the valve is opened, and cell sap enters the tank body through the feeding hole; thirdly, the motor is fixed on the top surface of the tank body, and the output shaft penetrates through the top surface of the tank body, so that the motor is fixed on the top surface of the tank body; thirdly, the stirring shaft is connected with the output shaft, the stirring shaft is embedded into the tank body, and the stirring shaft and the output shaft do not rotate relatively, so that the effect that the output shaft drives the stirring shaft to rotate is realized; thirdly, the stirring shaft drives the blades to rotate by connecting at least two blades with the outer wall of the stirring shaft; thirdly, through setting up the bin outlet in the bottom of jar body, the bin outlet is linked together with jar internal portion to be linked together solenoid valve and bin outlet, realized opening the effect that solenoid valve cell liquid discharged through the bin outlet. By adopting the structure, cell sap is poured into the funnel, the cell sap is filtered by the filter paper, the cell sap permeates the filter paper, penetrates through the filter holes in the filter plate and enters the connecting pipe, the cell sap enters the feeding hole through the connecting pipe, the valve is opened, the cell sap enters the tank body, the contact between the filtered cell sap and air is avoided, and the stability of the cell sap is ensured; the motor moves, the output shaft drives the stirring shaft to move, so that the paddle is driven to rotate to stir the cell sap, the cell sap is subjected to the action of preliminary cell separation by centrifugal force, the technical effect of cell sap layering is realized after the cell sap is static for a period of time, and the working efficiency is improved; the electromagnetic valve is opened to discharge the cell sap through the discharge hole, and the separated cell sap is collected, so that the applicability of the device is improved.
In the above technical solution, preferably, the adipose-derived mesenchymal stem cell separation device further includes: a sampling port and a sampling tube; the sampling port is arranged on the top surface of the tank body and communicated with the interior of the tank body; at least part of the sampling tube is inserted into the sampling port.
In the technical scheme, the sampling port is arranged on the top surface of the tank body and communicated with the inside of the tank body, so that the effect of sampling through the sampling port is realized; thirdly, through with at least part of sampling tube embedding sample connection, realized stretching into the sample connection with the sampling tube to the effect to the cell sap sample after the internal portion separation of jar has improved the suitability of device.
In the above technical solution, preferably, the adipose-derived mesenchymal stem cell separation device further includes: an observation window; the observation window is arranged on the side surface of the tank body.
In the technical scheme, the observation window is arranged on the side surface of the tank body, so that the cell liquid separation progress is observed through the observation window, and the applicability of the device is improved.
In the above technical solution, preferably, the funnel further includes: a filtering part and a flow guide part; the filtering part is in a step shape, and at least part of the filtering part is arranged on the top surface of the supporting plate; the guide part is in a step shape, the guide part is connected with the bottom of the filtering part, and the maximum diameter of the guide part is smaller than the minimum diameter of the filtering part.
In the technical scheme, the filtering part is in a step shape, and at least part of the filtering part is arranged on the top surface of the supporting plate, so that the filtering part can filter cell sap; and thirdly, the flow guide part is in a step shape, the flow guide part is connected with the bottom of the filtering part, and the maximum diameter of the flow guide part is smaller than the minimum diameter of the filtering part, so that the cell sap flows down along the flow guide part.
In the above technical solution, preferably, the adipose-derived mesenchymal stem cell separation device further includes: a dust cover; the dust cover is fixed on the top surface of the supporting plate, and the bottom surface of the dust cover is attached to the top surface of the funnel.
In this technical scheme, through fixing the top surface at the backup pad with the shield, and the bottom surface of shield laminates with the top surface of funnel mutually, has realized that the shield laminates with the funnel top surface mutually, compresses tightly the funnel, the effect of fixed funnel position, and prevents that the dust from getting into the funnel.
In the above technical solution, preferably, the adipose-derived mesenchymal stem cell separation device further includes: a support frame; the two support frames are respectively fixedly connected with the two sides of the tank body.
In the technical scheme, the two support frames are respectively fixedly connected with the two sides of the tank body, so that the support frames can play a role in fixedly supporting the tank body, and the stability of the device is improved.
Additional aspects and advantages of the utility model will be set forth in part in the description which follows and, in part, will be obvious from the description, or may be learned by practice of the utility model.
Drawings
The above and/or additional aspects and advantages of the present invention will become apparent and readily appreciated from the following description of the embodiments, taken in conjunction with the accompanying drawings of which:
fig. 1 illustrates a schematic structural view of an adipose-derived mesenchymal stem cell separation device according to an embodiment of the present invention;
fig. 2 is a schematic structural view at a of the structural view of the adipose-derived mesenchymal stem cell separation device of fig. 1 according to an embodiment of the present invention;
fig. 3 illustrates a schematic structural view of an adipose-derived mesenchymal stem cell separation device according to another embodiment of the present invention;
fig. 4 illustrates a schematic configuration diagram of an adipose-derived mesenchymal stem cell-separating device according to still another embodiment of the present invention;
fig. 5 illustrates a schematic configuration diagram of an adipose-derived mesenchymal stem cell-separating device according to still another embodiment of the present invention;
wherein, the correspondence between the reference numbers and the part names in fig. 1 to 5 is:
10 jar body, 12 pillars, 14 backup pads, 16 funnels, 161 filter house, 162 water conservancy diversion portion, 18 filter boards, 20 filter paper, 22 connecting pipes, 24 feed inlets, 26 valves, 28 motors, 30 (mixing) shaft, 32 paddle, 34 discharge gate, 36 solenoid valves, 38 sample connection, 40 sampling tube, 42 observation window, 44 shield, 46 support frames.
Detailed Description
In order that the above objects, features and advantages of the present invention can be more clearly understood, a more particular description of the utility model will be rendered by reference to the appended drawings. It should be noted that the embodiments and features of the embodiments of the present application may be combined with each other without conflict.
In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention, however, the present invention may be practiced in other ways than those specifically described herein, and therefore the scope of the present invention is not limited by the specific embodiments disclosed below.
The adipose-derived mesenchymal stem cell separating device according to some embodiments of the present invention is described below with reference to fig. 1 to 5.
In an embodiment of the present invention, as shown in fig. 1 to 5, the present invention provides an adipose-derived mesenchymal stem cell separating device, including: the device comprises a tank body 10, a support column 12, a support plate 14, a funnel 16, a filter plate 18, filter paper 20, a connecting pipe 22, a feed inlet 24, a valve 26, a motor 28, a stirring shaft 30, a blade 32, a discharge outlet 34 and an electromagnetic valve 36; the tank body 10 is a hollow cavity; four pillars 12 are respectively fixed on the top surface of the tank body 10; the supporting plate 14 is connected with the top surfaces of the four pillars 12, and through holes are arranged on the supporting plate 14 in a penetrating manner; the funnel 16 is arranged on the top surface of the support plate 14, and at least part of the funnel 16 is embedded in the through hole; the filter plate 18 is provided with at least two filter holes, and the filter plate 18 is embedded in the funnel 16; the filter paper 20 is placed on the filter plate 18; one end of the connecting pipe 22 is communicated with the bottom of the funnel 16; the feed inlet 24 is arranged on the top surface of the tank body 10, the feed inlet 24 is communicated with the interior of the tank body 10, and the feed inlet 24 is communicated with the other end of the connecting pipe 22; one end of the valve 26 is communicated with the feeding port 24; the motor 28 comprises an output shaft, the motor 28 is fixed on the top surface of the tank body 10, and the output shaft penetrates through the top surface of the tank body 10; the stirring shaft 30 is connected with the output shaft, the stirring shaft 30 is embedded in the tank body 10, and the stirring shaft 30 and the output shaft do not rotate relatively; at least two blades 32 are connected with the outer wall of the stirring shaft 30; the discharge port 34 is arranged at the bottom of the tank body 10, and the discharge port 34 is communicated with the inside of the tank body 10; a solenoid valve 36 is in communication with the discharge port 34.
In this embodiment, first, the tank 10 is a hollow cavity, and the four pillars 12 are respectively fixed on the top surface of the tank 10, so that the four pillars 12 are connected to the top surface of the tank 10; secondly, the supporting plate 14 is connected with the top surfaces of the four pillars 12, and through holes are arranged on the supporting plate 14 in a penetrating manner, so that the supporting plate 14 is connected with the top surfaces of the pillars 12; again, by positioning the funnel 16 on the top surface of the support plate 14 with at least a portion of the funnel 16 inserted into the through-hole, the function of fixing the position of the funnel 16 is achieved; thirdly, the filter plate 18 is provided with at least two filter holes, the filter plate 18 is embedded in the funnel 16, and the filter paper 20 is placed on the filter plate 18, so that the cell sap is filtered by the filter paper 20; thirdly, the funnel 16 is communicated with the connecting pipe 22 by communicating one end of the connecting pipe 22 with the bottom of the funnel 16; thirdly, the cell sap enters the feed inlet 24 through the connecting pipe 22 and then enters the tank body 10 by arranging the feed inlet 24 on the top surface of the tank body 10, communicating the feed inlet 24 with the inside of the tank body 10 and communicating the feed inlet 24 with the other end of the connecting pipe 22; thirdly, one end of the valve 26 is communicated with the feed port 24, so that the valve 26 is opened, and the cell sap enters the tank body 10 through the feed port 24; thirdly, the motor 28 is fixed on the top surface of the tank body 10 by fixing the motor 28 on the top surface of the tank body 10, and the output shaft penetrates through the top surface of the tank body 10; thirdly, the stirring shaft 30 is connected with the output shaft, the stirring shaft 30 is embedded into the tank body 10, and the stirring shaft 30 and the output shaft do not rotate relatively, so that the effect that the output shaft drives the stirring shaft 30 to rotate is realized; thirdly, the stirring shaft 30 drives the blades 32 to rotate by connecting at least two blades 32 with the outer wall of the stirring shaft 30; again, by disposing the discharge port 34 at the bottom of the can body 10, the discharge port 34 communicates with the inside of the can body 10, and communicating the electromagnetic valve 36 with the discharge port 34, the effect of opening the electromagnetic valve 36 to discharge the cell liquid through the discharge port 34 is achieved. By adopting the structure, cell sap is poured into the funnel 16, the cell sap is filtered by the filter paper 20, the cell sap permeates the filter paper 20, penetrates through the filter holes on the filter plate 18 and enters the connecting pipe 22, the cell sap enters the feeding hole 24 through the connecting pipe 22, the valve 26 is opened, the cell sap enters the tank body 10, the contact between the filtered cell sap and air is avoided, and the stability of the cell sap is ensured; the motor 28 moves, the output shaft drives the stirring shaft 30 to move, so that the paddle 32 is driven to rotate to stir the cell sap, the cell sap is subjected to the action of primary cell separation by centrifugal force, the technical effect of cell sap layering is realized after the cell sap is static for a period of time, and the working efficiency is improved; the electromagnetic valve 36 is opened to discharge the cell sap through the discharge port 34, and the separated cell sap is collected, thereby improving the applicability of the apparatus.
In an embodiment of the present invention, preferably, as shown in fig. 1, the adipose-derived mesenchymal stem cell separation device further includes: a sampling port 38 and a sampling tube 40; the sampling port 38 is arranged on the top surface of the tank body 10, and the sampling port 38 is communicated with the interior of the tank body 10; at least a portion of the sampling tube 40 is inserted into the sampling port 38.
In this embodiment, by disposing the sampling port 38 on the top surface of the can body 10, the sampling port 38 communicates with the inside of the can body 10, so that the sampling function through the sampling port 38 is realized; thirdly, through embedding at least part of the sampling tube 40 into the sampling port 38, the sampling tube 40 is stretched into the sampling port 38, so that the cell sap after the separation in the tank body 10 is sampled, and the applicability of the device is improved.
In an embodiment of the present invention, preferably, as shown in fig. 3, the adipose-derived mesenchymal stem cell separation device further includes: a viewing window 42; the observation window 42 is provided at the side of the can body 10.
In this embodiment, by providing the observation window 42 on the side surface of the tank 10, the observation of the progress of the separation of the cell fluid through the observation window 42 is performed, and the applicability of the apparatus is improved.
In an embodiment of the present invention, preferably, as shown in fig. 2, the hopper 16 further includes: a filter portion 161 and a guide portion 162; the filter part 161 is of a stepped type, and at least a part of the filter part 161 is disposed on the top surface of the support plate 14; the flow guide part 162 is stepped, the flow guide part 162 is connected with the bottom of the filtering part 161, and the maximum diameter of the flow guide part 162 is smaller than the minimum diameter of the filtering part 161.
In this embodiment, the filtration of the cell sap by the filter portions 161 is achieved by the filter portions 161 being stepped, at least a part of the filter portions 161 being provided on the top surface of the support plate 14; thirdly, the flow guide part 162 is stepped, the flow guide part 162 is connected with the bottom of the filtering part 161, and the maximum diameter of the flow guide part 162 is smaller than the minimum diameter of the filtering part 161, so that the cell sap flows down along the flow guide part 162.
In an embodiment of the present invention, preferably, as shown in fig. 3, the adipose-derived mesenchymal stem cell separation device further includes: a dust cover 44; the dust cap 44 is secured to the top surface of the support plate 14, and the bottom surface of the dust cap 44 abuts the top surface of the funnel 16.
In this embodiment, the dust cap 44 is attached to the top surface of the support plate 14, and the bottom surface of the dust cap 44 is attached to the top surface of the hopper 16, thereby compressing the hopper 16, fixing the position of the hopper 16, and preventing dust from entering the hopper 16.
In an embodiment of the present invention, preferably, as shown in fig. 1, the adipose-derived mesenchymal stem cell separation device further includes: a support frame 46; the two supporting frames 46 are respectively fixedly connected with the two sides of the tank body 10.
In this embodiment, the two support frames 46 are respectively and fixedly connected with the two sides of the tank 10, so that the support frames 46 can fixedly support the tank 10, and the stability of the device is improved.
In the description of the present invention, the terms "plurality" or "a plurality" refer to two or more, and unless otherwise specifically limited, the terms "upper", "lower", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are merely for convenience in describing the present invention and simplifying the description, but do not indicate or imply that the referred device or element must have a specific orientation, be constructed in a specific orientation, and be operated, and thus should not be construed as limiting the present invention; the terms "connected," "mounted," "secured," and the like are to be construed broadly and include, for example, fixed connections, removable connections, or integral connections; may be directly connected or indirectly connected through an intermediate. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.
In the description of the present invention, the description of the terms "one embodiment," "some embodiments," "specific embodiments," etc., means that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the present invention. In the present invention, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (6)
1. An adipose-derived mesenchymal stem cell separation device, which is characterized by comprising:
the tank body is a hollow cavity;
the four supporting columns are respectively fixed on the top surface of the tank body;
the supporting plate is connected with the top surfaces of the four supporting columns, and through holes are formed in the supporting plate in a penetrating mode;
the funnel is arranged on the top surface of the supporting plate, and at least part of the funnel is embedded into the through hole;
the filter plate is provided with at least two filter holes and is embedded in the funnel;
a filter paper placed on the filter plate;
one end of the connecting pipe is communicated with the bottom of the funnel;
the feed inlet is arranged on the top surface of the tank body, communicated with the interior of the tank body and communicated with the other end of the connecting pipe;
one end of the valve is communicated with the feeding hole;
the motor comprises an output shaft, the motor is fixed on the top surface of the tank body, and the output shaft penetrates through the top surface of the tank body;
the stirring shaft is connected with the output shaft, is embedded into the tank body and does not rotate relative to the output shaft;
the at least two blades are connected with the outer wall of the stirring shaft;
the discharge port is arranged at the bottom of the tank body and communicated with the inside of the tank body;
and the electromagnetic valve is communicated with the discharge port.
2. The adipose-derived mesenchymal stem cell separation device of claim 1, further comprising:
the sampling port is arranged on the top surface of the tank body, and the sampling port is communicated with the inside of the tank body;
a sampling tube, at least part of the sampling tube is embedded in the sampling port.
3. The adipose-derived mesenchymal stem cell separation device according to claim 2, further comprising:
the observation window is arranged on the side face of the tank body.
4. The adipose-derived mesenchymal stem cell separation device of claim 3, wherein the funnel further comprises:
the filtering part is of a step type, and at least part of the filtering part is arranged on the top surface of the supporting plate;
the water conservancy diversion portion, the water conservancy diversion portion is the notch cuttype, the water conservancy diversion portion with the bottom of filter house is connected, just the maximum diameter of water conservancy diversion portion is less than the minimum diameter of filter house.
5. The adipose-derived mesenchymal stem cell separation device according to claim 4, further comprising:
the dustproof cover is fixed on the top surface of the supporting plate, and the bottom surface of the dustproof cover is attached to the top surface of the funnel.
6. The adipose-derived mesenchymal stem cell separation device according to claim 5, wherein:
the support frames are respectively and fixedly connected with the two sides of the tank body.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122032302.5U CN215856041U (en) | 2021-08-26 | 2021-08-26 | Adipose tissue-derived stem cell separation device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122032302.5U CN215856041U (en) | 2021-08-26 | 2021-08-26 | Adipose tissue-derived stem cell separation device |
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| Publication Number | Publication Date |
|---|---|
| CN215856041U true CN215856041U (en) | 2022-02-18 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202122032302.5U Active CN215856041U (en) | 2021-08-26 | 2021-08-26 | Adipose tissue-derived stem cell separation device |
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| CN (1) | CN215856041U (en) |
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2021
- 2021-08-26 CN CN202122032302.5U patent/CN215856041U/en active Active
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