CN115436514B - Folic acid content detection device - Google Patents
Folic acid content detection device Download PDFInfo
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- CN115436514B CN115436514B CN202211076709.0A CN202211076709A CN115436514B CN 115436514 B CN115436514 B CN 115436514B CN 202211076709 A CN202211076709 A CN 202211076709A CN 115436514 B CN115436514 B CN 115436514B
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- China
- Prior art keywords
- folic acid
- pipe
- acid content
- content detection
- tank
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- OVBPIULPVIDEAO-LBPRGKRZSA-N folic acid Chemical compound C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-LBPRGKRZSA-N 0.000 title claims abstract description 65
- 238000001514 detection method Methods 0.000 title claims abstract description 40
- OVBPIULPVIDEAO-UHFFFAOYSA-N N-Pteroyl-L-glutaminsaeure Natural products C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)NC(CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-UHFFFAOYSA-N 0.000 title claims abstract description 32
- 235000019152 folic acid Nutrition 0.000 title claims abstract description 32
- 239000011724 folic acid Substances 0.000 title claims abstract description 32
- 229960000304 folic acid Drugs 0.000 title claims abstract description 32
- 239000007788 liquid Substances 0.000 claims abstract description 49
- 238000002156 mixing Methods 0.000 claims abstract description 42
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 claims abstract description 22
- 235000011114 ammonium hydroxide Nutrition 0.000 claims abstract description 22
- 239000007853 buffer solution Substances 0.000 claims abstract description 22
- 238000003756 stirring Methods 0.000 claims description 22
- 239000000463 material Substances 0.000 claims description 19
- 239000002893 slag Substances 0.000 claims description 17
- 238000007789 sealing Methods 0.000 claims description 15
- 239000000203 mixture Substances 0.000 claims description 9
- 239000013049 sediment Substances 0.000 claims description 7
- 239000011148 porous material Substances 0.000 claims description 3
- 239000012528 membrane Substances 0.000 claims description 2
- 230000006978 adaptation Effects 0.000 claims 1
- 239000000243 solution Substances 0.000 description 11
- 238000001914 filtration Methods 0.000 description 10
- 239000012488 sample solution Substances 0.000 description 10
- 241000220317 Rosa Species 0.000 description 9
- 239000000523 sample Substances 0.000 description 8
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 229930003761 Vitamin B9 Natural products 0.000 description 1
- QGZKDVFQNNGYKY-UHFFFAOYSA-N ammonia Natural products N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000001185 bone marrow Anatomy 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 235000021022 fresh fruits Nutrition 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 238000005374 membrane filtration Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000009965 odorless effect Effects 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 150000003195 pteridines Chemical class 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 235000019159 vitamin B9 Nutrition 0.000 description 1
- 239000011727 vitamin B9 Substances 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
Abstract
The invention relates to the technical field of folic acid detection, in particular to a folic acid content detection device, which comprises a detection table, wherein a mixing tank is fixedly arranged at the upper end of the detection table, a liquid chromatograph is fixedly arranged at one side position of the upper end of the detection table, which is close to the mixing tank, a buffer solution tank is arranged at one side of the upper end of the mixing tank, an ammonia water tank is arranged at the other side of the upper end of the mixing tank, liquid through pipes are fixedly connected between the buffer solution tank and the ammonia water tank as well as the mixing tank, flow sensors are fixedly arranged on the liquid through pipes, and a filter box is fixedly communicated with a feed port of the liquid chromatograph through the liquid through pipes.
Description
Technical Field
The invention relates to the technical field of folic acid detection, in particular to a folic acid content detection device.
Background
Folic acid is a derivative of pteridine, which is initially separated from liver, and is found to be very abundant in green leaves of plants, so that the folic acid is named as folic acid, and folic acid is also called vitamin B9, is a water-soluble vitamin, has the effect of promoting the maturation of young cells in bone marrow, is widely existing in meat, fresh fruits and vegetables, is yellow crystalline powder, is odorless, is insoluble in alcohol, diethyl ether and other organic solvents.
In the prior art, when the folic acid content is detected, firstly, a buffer solution and an ammonia water solution are sequentially added into a mixing tank for containing a sample to be detected for mixing, and the buffer solution and the ammonia water solution are quantitatively added in a handheld container mode, so that manual operation of personnel in the folic acid content detection process is complicated, the labor capacity of personnel is increased, and the purity of the sample solution is reduced due to the fact that the sample solution is not sufficiently filtered, so that the folic acid content detection effect is affected.
Disclosure of Invention
The invention aims to provide a folic acid content detection device so as to solve the technical problems in the background technology.
In order to achieve the above purpose, the present invention provides the following technical solutions:
the utility model provides a folic acid content detection device, includes the detection platform, the upper end fixed mounting of detection platform has the blending tank, one side position department fixed mounting that the upper end of detection platform is close to the blending tank has the liquid chromatograph, upper end one side of blending tank is provided with the buffer solution jar, the upper end opposite side of blending tank is provided with the ammonia water jar, all fixedly connected with logical liquid pipe between buffer solution jar and ammonia water jar and the blending tank, fixedly provided with flow sensor on the logical liquid pipe, one side position department that is close to flow sensor on the logical liquid pipe is fixed and is provided with the solenoid valve, the feed port of liquid chromatograph has the rose box through logical material pipe fixed intercommunication, the upper end of rose box is provided with the suction pump, the inside fixed mounting of rose box has the inner filter layer, the inside of rose box is located the outside position department fixed mounting of inner filter layer has the outer filter layer, the rose box is through leading to expect the liquid port of pump fixedly connected with the feed through pipe, the feed through pipe fixedly communicates with the drain pipe, the feed through pipe of suction pump has the guide tube, the end fixed connection in the electric property of motor is located the end of mixer, the input end of flow controller, the input controller is connected with the input end of flow controller.
Preferably, the surface of the inner filter layer is provided with inner filter micropores, the surface of the outer filter layer is provided with outer filter micropores, and the pore size of the outer filter micropores is smaller than that of the inner filter micropores.
Through setting up interior filtration micropore and outer filtration micropore, reached the purpose that can carry out abundant filtration treatment to the sample solution of process.
Preferably, the inner filter layer and the outer filter layer are both a membrane filter layer member.
Preferably, one end of the mixing tank is fixedly provided with a motor, a driving shaft of the motor is fixedly connected with a stirring frame, a plurality of stirring sheets are fixedly connected to the stirring frame at intervals, and a plurality of material through holes are formed in the surfaces of the stirring sheets at intervals.
Through setting up stirring frame and stirring piece, reached the purpose of being convenient for to sample, buffer solution and the aqueous ammonia that await measuring in the blending tank stir the mixing treatment.
Preferably, the material guiding pipe is fixedly provided with a switch valve.
Through setting up the ooff valve, reached the purpose that can be convenient for control the passage state of passage pipe.
Preferably, the middle of the upper end of the mixing tank is fixedly communicated with a feeding pipe, and the upper end of the feeding pipe is in threaded connection with a sealing top cover.
Through setting up the inlet pipe, reached can add the purpose that awaits measuring the sample in the blending tank through the inlet pipe.
Preferably, the bottom end of the mixing tank is fixedly communicated with a slag liquid pipe, and the upper end of the slag liquid pipe is in threaded connection with a sealing bottom cover.
Through setting up the sediment liquid pipe, reached the purpose of being convenient for with the sediment liquid discharge of remaining in the blending tank.
Preferably, a slag liquid box is placed on the detection table, and an opening is formed in the surface of the slag liquid box.
Through setting up sediment liquid case and uncovered, reached the purpose that can collect the sediment liquid of discharging in the blending tank through sediment liquid case.
Preferably, a limiting block is fixedly arranged at the bottom end of the slag liquid box, a limiting groove is formed in the surface of the detection table, and the limiting block is matched with the limiting groove.
Through setting up stopper and spacing groove, reached the purpose of being convenient for carry out the location with the sediment liquid case and place on the test bench.
Preferably, a sealing ring is fixedly arranged between the mixing tank and the material guiding pipe, and the sealing ring is a member made of rubber.
By arranging the sealing ring, the aim of improving the tightness between the mixing tank and the material guiding pipe is fulfilled.
Compared with the prior art, the invention has the beneficial effects that:
according to the invention, the buffer solution and the ammonia water solution are conveniently quantitatively added into the mixing tank, so that the traditional hand-held container mode is replaced to add the buffer solution and the ammonia water solution, the operation of personnel is more convenient when the folic acid content is detected, the labor amount of the personnel is reduced, the sample solution can be sufficiently filtered, the purity of the sample solution is improved, and the folic acid content detection effect is ensured.
Drawings
FIG. 1 is a schematic perspective view of a folic acid content detection apparatus;
FIG. 2 is a schematic cross-sectional view of a folic acid content detecting apparatus;
FIG. 3 is an enlarged schematic view of the portion A in FIG. 2;
FIG. 4 is an enlarged schematic view of the portion B of FIG. 2;
fig. 5 is a schematic block diagram of a controller in a folic acid content detection apparatus.
In the figure: 1. a detection table; 11. a limit groove; 2. a mixing tank; 21. a motor; 22. a stirring rack; 23. stirring sheets; 24. a material opening; 25. a feed pipe; 26. a slag liquid pipe; 3. a liquid chromatograph; 31. a material passing pipe; 32. a controller; 4. a buffer solution tank; 5. an ammonia water tank; 6. a filter box; 61. a material pump; 62. a material guiding pipe; 63. a material guiding pipe; 64. an inner filter layer; 65. an outer filter layer; 66. internal filtration of micropores; 67. external filtering micropores; 7. a liquid pipe; 71. a flow sensor; 72. an electromagnetic valve; 8. a slag liquid box; 81. a limiting block; 9. and (3) sealing rings.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Referring to fig. 1-5, the present invention provides a technical solution:
the utility model provides a folic acid content detection device, including detecting bench 1, detecting bench 1's upper end fixed mounting has mix tank 2, one side position department fixed mounting that the upper end of detecting bench 1 is close to mix tank 2 has liquid chromatograph 3, mix tank 2's upper end one side is provided with buffer solution jar 4, mix tank 2's upper end opposite side is provided with ammonia water jar 5, all fixedly connected with liquid-passing tube 7 between buffer solution jar 4 and ammonia water jar 5 and the mix tank 2, fixedly provided with flow sensor 71 on the liquid-passing tube 7, one side position department fixed connection that is close to flow sensor 71 on the liquid-passing tube 7 is provided with solenoid valve 72, liquid chromatograph 3's feed port is through the fixed intercommunication of feed-through tube 31 has rose box 6, the upper end of rose box 6 is provided with pump 61, the inside fixed mounting of rose box 6 has inner filter layer 64, the inside of rose box 6 is located outside position department fixed mounting of inner filter layer 64 has outer filter layer 65, filter box 6 is through the liquid-passing port of leading to 62 fixed connection in pump 61, the feed-through tube of pump 61, the feed-through port fixed connection has guide tube 63 to the feed-through of pump 61, the feed-through tube is fixed connection to the guide tube, be provided with solenoid valve 32 in the electric motor 32 at the end of the side of mixer 2, electric controller 32 fixed connection 32 of feed-through end of feed-through 32, the input end of liquid chromatograph 3, the input end of 32.
As a preferred embodiment in this example, the surface of the inner filter layer 64 is provided with the inner filter micropores 66, the surface of the outer filter layer 65 is provided with the outer filter micropores 67, and the pore size of the outer filter micropores 67 is smaller than that of the inner filter micropores 66, so that the purpose of fully filtering the passing sample solution is achieved.
As a preferred implementation in this example, both the inner filter layer 64 and the outer filter layer 65 are a membrane filtration layer member.
As a preferred implementation manner in the embodiment, a motor 21 is fixedly installed at one end of the mixing tank 2, a driving shaft of the motor 21 is fixedly connected with a stirring frame 22, a plurality of stirring plates 23 are fixedly connected to the stirring frame 22 at intervals, and a plurality of material inlets 24 are formed in the surfaces of the stirring plates 23 at intervals, so that the purpose of stirring and mixing the sample to be measured, the buffer solution and the ammonia water in the mixing tank 2 is achieved.
As a preferred implementation manner in this embodiment, the on-off valve is fixedly disposed on the material guiding pipe 63, so as to achieve the purpose of being capable of conveniently controlling the circulation state of the material guiding pipe 63.
As a preferred implementation manner in the embodiment, a feeding pipe 25 is fixedly communicated with the middle of the upper end of the mixing tank 2, and a sealing top cover is connected with the upper end of the feeding pipe 25 in a threaded manner, so that the purpose of adding a sample to be measured into the mixing tank 2 through the feeding pipe 25 is achieved.
As a preferred implementation manner in this embodiment, the bottom end of the mixing tank 2 is fixedly connected with a slag liquid pipe 26, and the upper end of the slag liquid pipe 26 is in threaded connection with a sealing bottom cover, so as to achieve the purpose of facilitating the discharge of residual slag liquid in the mixing tank 2.
As a preferred embodiment of the present embodiment, the slag-liquid tank 8 is placed on the detection table 1, and the surface of the slag-liquid tank 8 is opened, so that the purpose of collecting the slag liquid discharged from the mixing tank 2 through the slag-liquid tank 8 is achieved.
As a preferred implementation manner in this embodiment, the bottom end of the slag liquid tank 8 is fixedly provided with the limiting block 81, the surface of the detection table 1 is provided with the limiting groove 11, and the limiting block 81 is matched with the limiting groove 11, so that the limiting block 81 is matched and inserted into the limiting groove 11, and the purpose of positioning and placing the slag liquid tank 8 on the detection table 1 is achieved.
As a preferred embodiment in this embodiment, a sealing ring 9 is fixedly disposed between the mixing tank 2 and the material guiding pipe 63, and the sealing ring 9 is a rubber member, so as to achieve the purpose of improving the sealing performance between the mixing tank 2 and the material guiding pipe 63.
Working principle:
in the process of using the device, a sample to be detected is added into the mixing tank 2 through the feed pipe 25, the addition amount of the buffer solution and the ammonia water solution is set, the buffer solution and the ammonia water solution are sequentially added into the mixing tank 2, the addition amount of the buffer solution and the ammonia water solution is monitored through the flow sensor 71, when the addition amount of the buffer solution and the ammonia water solution reaches a preset value, the controller 32 controls the electromagnetic valve 72 to be closed, so that the addition of the buffer solution and the ammonia water solution can be stopped timely, the motor 21 is used for driving the stirring frame 22 to rotate, the stirring sheets 23 on the stirring frame 22 are used for stirring the sample to be detected, the buffer solution and the ammonia water in the mixing tank 2, the sample to be detected, the buffer solution and the ammonia water are mixed to generate the sample solution, the sample solution to be detected is pumped into the filtering tank 6 through the pumping pump 61, the sample solution to be detected enters the liquid chromatograph 3 after the pre-filtering treatment of the inner filtering layer 64, and finally the folic acid content in the sample solution is filtered again through the outer filtering layer 65 and enters the liquid chromatograph 3.
The foregoing has shown and described the basic principles, principal features and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the above-described embodiments, and that the above-described embodiments and descriptions are only preferred embodiments of the present invention, and are not intended to limit the invention, and that various changes and modifications may be made therein without departing from the spirit and scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (10)
1. Folic acid content detection device, including detecting platform (1), its characterized in that: the utility model discloses a liquid chromatograph, including detection platform (1), feed-through pipe (61) and filter tank (6), mix jar (2) fixed mounting has upper end fixed mounting of detection platform (1), the upper end of detection platform (1) is close to one side position department fixed mounting of mix jar (2) has liquid chromatograph (3), upper end one side of mix jar (2) is provided with buffer solution jar (4), the upper end opposite side of mix jar (2) is provided with ammonia water jar (5), equal fixedly connected with feed-through pipe (7) between buffer solution jar (4) and ammonia water jar (5) and mix jar (2), fixedly provided with flow sensor (71) on feed-through pipe (7), one side position department fixed mounting that is close to flow sensor (71) on feed-through pipe (7) is provided with solenoid valve (72), the feed port of liquid chromatograph (3) is through feed-through pipe (31) fixed intercommunication has filter tank (6), the upper end of filter tank (6) is provided with pump (61), the inside fixed mounting of filter tank (6) has inlayer filter layer (64), be located inside pump (6) filter layer (62) and filter layer (61) is fixedly connected with feed-through pump (61), one end of the material guiding pipe (63) is fixedly communicated with the bottom side of the mixing tank (2), a controller (32) is fixedly arranged on the side wall of the liquid chromatograph (3), the output end of the flow sensor (71) is electrically connected with the input end of the controller (32), and the output end of the controller (32) is electrically connected with the input ends of the motor (21), the material sucking pump (61) and the electromagnetic valve (72).
2. A folic acid content detection apparatus according to claim 1, characterized in that: an inner filter micropore (66) is formed in the surface of the inner filter layer (64), an outer filter micropore (67) is formed in the surface of the outer filter layer (65), and the pore size of the outer filter micropore (67) is smaller than that of the inner filter micropore (66).
3. A folic acid content detection apparatus according to claim 2, characterized in that: the inner filter layer (64) and the outer filter layer (65) are both a membrane filter layer member.
4. A folic acid content detection apparatus according to claim 1, characterized in that: one end fixed mounting of blending tank (2) has motor (21), the drive shaft fixedly connected with stirring frame (22) of motor (21), interval fixedly connected with a plurality of stirring piece (23) on stirring frame (22), the surface interval of stirring piece (23) is equipped with a plurality of feed inlets (24).
5. A folic acid content detection apparatus according to claim 1, characterized in that: the material guide pipe (63) is fixedly provided with a switch valve.
6. A folic acid content detection apparatus according to claim 1, characterized in that: the middle of the upper end of the mixing tank (2) is fixedly communicated with a feeding pipe (25), and the upper end of the feeding pipe (25) is connected with a sealing top cover in a threaded manner.
7. The folic acid content detection apparatus as set forth in claim 6, wherein: the bottom end of the mixing tank (2) is fixedly communicated with a slag liquid pipe (26), and the upper end of the slag liquid pipe (26) is in threaded connection with a sealing bottom cover.
8. A folic acid content detection apparatus according to claim 1, characterized in that: the slag liquid box (8) is placed on the detection table (1), and an opening is formed in the surface of the slag liquid box (8).
9. A folic acid content detection apparatus as claimed in claim 8, wherein: the bottom fixed mounting of sediment liquid case (8) has stopper (81), spacing groove (11) have been seted up on the surface of detecting platform (1), stopper (81) and spacing groove (11) looks adaptation.
10. A folic acid content detection apparatus according to claim 1, characterized in that: a sealing ring (9) is fixedly arranged between the mixing tank (2) and the material guide pipe (63), and the sealing ring (9) is a member made of rubber.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211076709.0A CN115436514B (en) | 2022-09-05 | 2022-09-05 | Folic acid content detection device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211076709.0A CN115436514B (en) | 2022-09-05 | 2022-09-05 | Folic acid content detection device |
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| Publication Number | Publication Date |
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| CN115436514A CN115436514A (en) | 2022-12-06 |
| CN115436514B true CN115436514B (en) | 2023-11-07 |
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| CN113267575A (en) * | 2021-05-13 | 2021-08-17 | 高培（广州）乳业有限公司 | Folic acid detection method |
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| TWI413771B (en) * | 2011-02-14 | 2013-11-01 | Univ Nat Cheng Kung | Method and kit for detecting folate |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113267575A (en) * | 2021-05-13 | 2021-08-17 | 高培（广州）乳业有限公司 | Folic acid detection method |
Non-Patent Citations (1)
| Title |
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| 高效液相色谱法测定叶酸片中叶酸含量;黄洁文;雷会雯;;首都食品与医药(12);全文 * |
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