CN107903299A - Protein-enriched device - Google Patents
Protein-enriched device Download PDFInfo
- Publication number
- CN107903299A CN107903299A CN201711445007.4A CN201711445007A CN107903299A CN 107903299 A CN107903299 A CN 107903299A CN 201711445007 A CN201711445007 A CN 201711445007A CN 107903299 A CN107903299 A CN 107903299A
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- CN
- China
- Prior art keywords
- protein
- enriched
- foam
- ball
- reducing mechanism
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 44
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 44
- 239000006260 foam Substances 0.000 claims abstract description 44
- 238000000498 ball milling Methods 0.000 claims abstract description 19
- 102000004190 Enzymes Human genes 0.000 claims description 7
- 108090000790 Enzymes Proteins 0.000 claims description 7
- 239000000376 reactant Substances 0.000 claims description 7
- 238000003756 stirring Methods 0.000 claims description 2
- 239000007788 liquid Substances 0.000 abstract description 16
- 239000006228 supernatant Substances 0.000 abstract description 9
- 238000000034 method Methods 0.000 abstract description 8
- 230000000694 effects Effects 0.000 abstract description 6
- 238000000926 separation method Methods 0.000 abstract description 3
- 238000005265 energy consumption Methods 0.000 abstract description 2
- 238000000605 extraction Methods 0.000 abstract 1
- 239000000463 material Substances 0.000 description 4
- 238000010521 absorption reaction Methods 0.000 description 3
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 2
- 239000013530 defoamer Substances 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000003670 easy-to-clean Effects 0.000 description 2
- 239000007791 liquid phase Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 229910052710 silicon Inorganic materials 0.000 description 2
- 239000010703 silicon Substances 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000005587 bubbling Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 229940042399 direct acting antivirals protease inhibitors Drugs 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000000137 peptide hydrolase inhibitor Substances 0.000 description 1
- 238000000751 protein extraction Methods 0.000 description 1
- 238000000108 ultra-filtration Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The present invention discloses a kind of protein-enriched device, mainly include housing, suction pump and foam separator, agitating device, centrifugal device, ball-milling device is set to realize the extraction of protein in housing, protein-enriched is realized by foam separator, only need to adjust by control device and driving device and can obtain supernatant to carry out break process and centrifugally operated to cell in stoste, process is simple, save manpower and time, and the protein-enriched liquid effect obtained using foam separation method is good, processing procedure is simple, treating capacity is big, and cost and energy consumption are relatively low.
Description
Technical field
The invention belongs to DNA techniques field, more particularly to protein-enriched device.
Background technology
Protein isolates and purifies the research hotspot that analysis is bio-separation analysis field in recent years, point of low-abundance protein
Analysis is one of emphasis and difficult point content of proteomics research with identification, and the albumen of important vital movement is undertaken in organism
All it is often low-abundance protein, but its extremely low content brings difficulty to follow-up analysis and detection, limits people to it
Research and understanding, therefore, the effective concentration for realizing low-abundance protein is to realize that its accurate is analyzed and the most important condition of identification
One of.Many aspects are directed to effective enrichment of sample actually during proteomics research.Foam fraction factor is root
According to the principle of absorption, the bubbling into the liquid containing surface reactive material, makes the surface reactive material in liquid be gathered in gas-liquid circle
On face (surface of bubble), froth bed is formed above main body of liquid, froth bed and liquid phase main body are separated, it is possible to reaches dense
Contracting surface reactive material (in froth bed) and the purpose for purifying liquid phase main body.
Operation to protein-enriched at present is centrifuged using kit or ultrafiltration mostly, and not only process is complicated, work
Work amount is big, and larger using the cost of kit, it is impossible to uses on a large scale.
The content of the invention
In order to solve the problems in the prior art, the purpose of the present invention is to, there is provided a kind of protein-enriched device.
To achieve the above object, the technical solution adopted by the present invention is：A kind of protein-enriched device, it is characterised in that
Superposed reducing mechanism is equipped with including housing, suction pump and foam separator, in the housing and positioned at lower part
Centrifugal device, the reducing mechanism include ball-milling device and agitating device, and the centrifugal device is equipped with automatically opening and closing, described
Reducing mechanism upper end is equipped with enzyme flusher and the driving device for driving ball-milling device and agitating device；It is described to crush dress
Put upper end and be equipped with feed inlet, lower end is equipped with the discharge port of the first valve of band, and the lower part connection ball-milling device of the feed inlet is described
Ball-milling device is fixedly connected with reducing mechanism upper end；The inlet of the suction pump passes through housing by telescoping tube, described flexible
The end of pipe can stretch up and down, and the end of the telescoping tube is equipped with level sensor, the end of the discharge port and telescoping tube
End be oppositely arranged with automatically opening and closing；The suction pump, centrifugal device, automatically opening and closing, the first valve, telescoping tube,
Level sensor regulates and controls by the control device for being arranged on hull outside, and level sensor is mainly believed to feed back liquid level surplus
Cease to control system, to facilitate the working status of control suction pump；Stoste is crushed by reducing mechanism, in crushing process
Can by enzyme flusher add protein suppress enzyme, to prevent protein to be decomposed, clasmatosis liquid enter centrifugal device in from
The heart extracts to obtain supernatant, and supernatant is pumped into after foam separator processing by telescoping tube by suction pump and obtains protein richness
Liquid collecting.
Further, the rotation axis of the agitating device is connected through ball-milling device with driving device, in the rotation axis
Equipped with spiral cutter blade, cell can further be crushed.
Further, it is rich to include foam reactant device, air pump, foam trap and protein for the foam separator
Collect collection, the foam trap is connected with protein-enriched collection by the conduit with the second valve.It is described
Foam separator is mainly to carry out protein-enriched to obtained supernatant.
Further, the foam reactant device is upside down funnel shape, its top enters in foam trap and is higher by foam
The bottom 20-30mm of collector, so designing is advantageous in that, bubble can be utilized in gas-liquid interface effect absorption supernatant
Protein, rises and becomes larger, into foam trap.
Further, blender is equipped with the foam trap, agitating paddle is carried on the blender, bubble enters bubble
After foam collector, by the collective effect of organic silicon defoamer and blender, defoaming rupture forms protein-enriched liquid.
Further, the centrifugal device is detachable apparatus, easy to clean.
Compared with prior art, the beneficial effects of the invention are as follows：Protein Extraction is completed using automated machine device
With the operation of enrichment, and the protein-enriched liquid effect drawn using foam separation method is good, and processing procedure is simple, treating capacity
Greatly, cost and energy consumption are relatively low.
Brief description of the drawings
Fig. 1 is present protein enriching apparatus structure diagram；
Fig. 2 is present protein foam separator structure diagram；
In figure：1st, housing；2nd, suction pump；3rd, foam separator；4th, reducing mechanism；5th, centrifugal device；6 ball-milling devices；
7th, agitating device；8th, automatically opening and closing；9th, enzyme flusher；10th, driving device；11st, feed inlet；12nd, the first valve；13rd, go out
Material mouth；14th, telescoping tube；15th, level sensor；16th, control device；17th, rotation axis；18th, spiral cutter blade；19th, foam is anti-
Answer device；20th, air pump；21st, foam trap；22nd, protein-enriched collection；23rd, the second valve；24th, conduit；25th, stir
Mix device.
Embodiment
Below in conjunction with the attached drawing in the present invention, technical scheme is clearly and completely described, it is clear that
Described embodiment is only part of the embodiment of the present invention, instead of all the embodiments.Based on the implementation in the present invention
Example, all other embodiment that those of ordinary skill in the art are obtained under the conditions of creative work is not made, belongs to
The scope of protection of the invention.
Embodiment 1, as shown in Figure 1, a kind of protein-enriched device, it is characterised in that including housing 1, suction pump 2 with
And foam separator 3, the housing 1 is interior to be equipped with superposed reducing mechanism 4 and the centrifugal device 5 positioned at lower part, described
Reducing mechanism 4 includes ball-milling device 6 and agitating device 7, and the centrifugal device 5 is equipped with automatically opening and closing 8, the centrifugal device
5 be detachable apparatus, and easy to clean, 4 upper end of reducing mechanism is equipped with enzyme flusher 9 and for driving ball-milling device 6
With the driving device 10 of agitating device 7；4 upper end of reducing mechanism is equipped with feed inlet 11, and lower end is equipped with the first valve of band 12
Discharge port 13, the lower part connection ball-milling device 6 of the feed inlet 11, the ball-milling device 6 is fixed with 4 upper end of reducing mechanism to be connected
Connect；The inlet of the suction pump 2 passes through housing 1 by telescoping tube 14, and the end of the telescoping tube 14 can stretch up and down, institute
The end for stating telescoping tube 14 is equipped with level sensor 15, the end of the discharge port 13 and the end of telescoping tube 14 with automatically
Switch cover 8 is oppositely arranged；The rotation axis 17 of the agitating device 7 is connected through ball-milling device 6 with driving device 10, the rotation
Axis 17 is equipped with spiral cutter blade 18, and cell further can be crushed；It is the suction pump 2, centrifugal device 5, automatic
Switch cover 8, the first valve 12, telescoping tube 14, level sensor 15 regulate and control by the control device 16 for being arranged on the outside of housing 1；
As shown in Fig. 2, the foam separator 3 includes foam reactant device 19, air pump 20, foam trap 21 and protein richness
Collect collection 22, the foam trap 21 passes through the conduit with the second valve 23 with protein-enriched collection 22
24 connections, the foam reactant device 19 are upside down funnel shape, its top enters in foam trap 21 and is higher by foam trap
21 bottom 20-30mm, so designing is advantageous in that, bubble can utilize the albumen in gas-liquid interface effect absorption supernatant
Matter, rises and becomes larger, into foam trap 21, and easy to make the protein-enriched liquid after bubbles burst be easy to receive
Collect, be equipped with blender 15 in the foam trap 21, agitating paddle is carried on the blender 25.
The operation principle of protein-enriched device in the present embodiment is:Stoste enters in ball-milling device 6 from feed inlet 11,
Cell is crushed by ball-milling device 6 and agitating device 7, and in crushing process, protease inhibitors is added by enzyme flusher 9,
Prevent protein to be decomposed, the first valve 12 and automatically opening and closing 8 are opened by control device 16, clasmatosis liquid is by discharge port
13 enter centrifugal device 5, close automatically opening and closing 8 and are centrifuged to obtain supernatant, open automatically opening and closing 8, control telescoping tube
14 enter centrifugal device 5, and control device 16 regulates and controls the working status of suction pump 2 by 15 feedack of level sensor, will
Supernatant is pumped into foam reactant device 19, and air pump 20 produces bubble, and bubble brings supernatant into from foam reactant device 19
In foam trap 21, by the collective effect of organic silicon defoamer and blender 25, defoaming rupture forms protein-enriched liquid,
The second valve 23 is opened, protein-enriched liquid is entered in protein-enriched collection 22 by conduit 24, completes whole protein
The course of work of enrichment.
Although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
Understanding without departing from the principles and spirit of the present invention can carry out these embodiments a variety of changes, modification, replace
And modification, the scope of the present invention is defined by the appended.
Claims (6)
- A kind of 1. protein-enriched device, it is characterised in that including housing (1), suction pump (2) and foam separator (3), Superposed reducing mechanism (4) and the centrifugal device (5) positioned at lower part, the reducing mechanism (4) are equipped with the housing (1) Including ball-milling device (6) and agitating device (7), the centrifugal device (5) is equipped with automatically opening and closing (8), the reducing mechanism (4) upper end is equipped with enzyme flusher (9) and the driving device (10) for driving ball-milling device (6) and agitating device (7)；Institute State reducing mechanism (4) upper end and be equipped with feed inlet (11), lower end is equipped with the discharge port (13) of the first valve of band (12), the feed inlet (11) lower part connection ball-milling device (6), the ball-milling device (6) are fixedly connected with reducing mechanism (4) upper end；The suction pump (2) inlet passes through housing (1) by telescoping tube (14), and the end of the telescoping tube (14) can stretch up and down, described flexible The end of pipe (14) is equipped with level sensor (15), the end of the discharge port (13) and the end of telescoping tube (14) with from Dynamic switch cover (8) is oppositely arranged；It is the suction pump (2), centrifugal device (5), automatically opening and closing (8), the first valve (12), flexible Pipe (14), level sensor (15) regulate and control by the control device (16) being arranged on the outside of housing (1).
- 2. protein-enriched device according to claim 1, it is characterised in that the rotation axis of the agitating device (7) (17) it is connected through ball-milling device (6) with driving device (10), the rotation axis (17) is equipped with spiral cutter blade (18).
- 3. protein-enriched device according to claim 1, it is characterised in that the foam separator (3) includes bubble Foam reactor (19), air pump (20), foam trap (21) and protein-enriched collection (22), the foam are collected Device (21) is connected with protein-enriched collection (22) by the conduit (24) with the second valve (23).
- 4. protein-enriched device according to claim 3, it is characterised in that the foam reactant device (19) is leaked to be inverted Bucket-shaped, its top enters in foam trap (21) and is higher by the bottom 20-30mm of foam trap (21).
- 5. protein-enriched device according to claim 3, it is characterised in that be equipped with and stir in the foam trap (21) Device (25) is mixed, agitating paddle is carried on the blender (25).
- 6. protein-enriched device according to claim 1, it is characterised in that the centrifugal device (5) is removed stage makeup and costume to be removable Put.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711445007.4A CN107903299A (en) | 2017-12-27 | 2017-12-27 | Protein-enriched device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711445007.4A CN107903299A (en) | 2017-12-27 | 2017-12-27 | Protein-enriched device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107903299A true CN107903299A (en) | 2018-04-13 |
Family
ID=61871619
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711445007.4A Withdrawn CN107903299A (en) | 2017-12-27 | 2017-12-27 | Protein-enriched device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107903299A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112295749A (en) * | 2020-10-20 | 2021-02-02 | 李宁 | High-speed centrifugal device for extracting natural products |
| CN118108377A (en) * | 2024-04-11 | 2024-05-31 | 东北农业大学 | A method for recovering water-soluble protein in corn starch processing wastewater |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3969336A (en) * | 1974-05-22 | 1976-07-13 | Abbott Laboratories | Method of separating and recovering soluble proteins from protein containing solutions employing foam fractionation |
| CN101081714A (en) * | 2006-05-31 | 2007-12-05 | 广州神阳高新技术有限公司 | Automatic lifting foam discharge horizontal control device |
| CN101805033A (en) * | 2009-02-17 | 2010-08-18 | 张志勇 | Automatic separation system and method for protein in liquid |
| CN202697682U (en) * | 2012-06-28 | 2013-01-30 | 湖北中烟工业有限责任公司 | Device for reducing content of protein in reconstituted tobaccos |
| CN203128549U (en) * | 2013-04-02 | 2013-08-14 | 石小燕 | Protein extracting device |
| CN203128550U (en) * | 2013-04-02 | 2013-08-14 | 石小燕 | Solar protein extraction device |
| CN206265643U (en) * | 2016-12-05 | 2017-06-20 | 广州悦康生物制药有限公司 | A kind of pharmaceutical purpose extraction element |
-
2017
- 2017-12-27 CN CN201711445007.4A patent/CN107903299A/en not_active Withdrawn
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3969336A (en) * | 1974-05-22 | 1976-07-13 | Abbott Laboratories | Method of separating and recovering soluble proteins from protein containing solutions employing foam fractionation |
| CN101081714A (en) * | 2006-05-31 | 2007-12-05 | 广州神阳高新技术有限公司 | Automatic lifting foam discharge horizontal control device |
| CN101805033A (en) * | 2009-02-17 | 2010-08-18 | 张志勇 | Automatic separation system and method for protein in liquid |
| CN202697682U (en) * | 2012-06-28 | 2013-01-30 | 湖北中烟工业有限责任公司 | Device for reducing content of protein in reconstituted tobaccos |
| CN203128549U (en) * | 2013-04-02 | 2013-08-14 | 石小燕 | Protein extracting device |
| CN203128550U (en) * | 2013-04-02 | 2013-08-14 | 石小燕 | Solar protein extraction device |
| CN206265643U (en) * | 2016-12-05 | 2017-06-20 | 广州悦康生物制药有限公司 | A kind of pharmaceutical purpose extraction element |
Non-Patent Citations (1)
| Title |
|---|
| 高洁 等: "泡沫分离技术发展现状及趋势" * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112295749A (en) * | 2020-10-20 | 2021-02-02 | 李宁 | High-speed centrifugal device for extracting natural products |
| CN118108377A (en) * | 2024-04-11 | 2024-05-31 | 东北农业大学 | A method for recovering water-soluble protein in corn starch processing wastewater |
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| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20180413 |
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| WW01 | Invention patent application withdrawn after publication |