CN211847897U - Pretreatment device for extracting DNA (deoxyribonucleic acid) of environmental sample - Google Patents
Pretreatment device for extracting DNA (deoxyribonucleic acid) of environmental sample Download PDFInfo
- Publication number
- CN211847897U CN211847897U CN202020114665.6U CN202020114665U CN211847897U CN 211847897 U CN211847897 U CN 211847897U CN 202020114665 U CN202020114665 U CN 202020114665U CN 211847897 U CN211847897 U CN 211847897U
- Authority
- CN
- China
- Prior art keywords
- shell
- support rails
- wall
- pretreatment device
- rail
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 230000007613 environmental effect Effects 0.000 title claims abstract description 18
- 102000053602 DNA Human genes 0.000 title description 12
- 108020004414 DNA Proteins 0.000 title description 12
- 239000002245 particle Substances 0.000 claims abstract description 14
- 238000007781 pre-processing Methods 0.000 claims abstract description 5
- 238000003756 stirring Methods 0.000 claims description 31
- 239000012535 impurity Substances 0.000 claims description 9
- 238000007400 DNA extraction Methods 0.000 claims description 8
- 239000007788 liquid Substances 0.000 abstract description 11
- 239000008187 granular material Substances 0.000 abstract description 4
- 238000002474 experimental method Methods 0.000 abstract description 2
- 238000000605 extraction Methods 0.000 abstract description 2
- 238000001914 filtration Methods 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Images
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The utility model discloses a preprocessing device for extraction of environmental sample DNA, which comprises a housin, the top of casing is provided with the feed inlet, the top and the casing roof through connection of feed inlet, casing inner both sides wall is by the fixed rail that supports of upper end one number, the upper end that supports the rail for two numbers is provided with large granule debris filter screen, the lower extreme that supports the rail for two numbers is provided with two numbers and supports the fixed setting of rail on the both sides wall of casing, and two numbers upper ends that support the rail are provided with the tiny particle debris filter screen, the lower extreme of tiny particle debris filter screen is provided with funnel type collection medium plate fixed connection on shells inner wall, the lower extreme of funnel type collection medium plate is provided with backup pad fixed connection on shells inner wall, the fixed agitator chamber that is provided with in upper end of backup pad. The device is provided with multichannel filter equipment, can get rid of the size graininess debris and the greasy dirt in the liquid environment sample, guarantees that environment sample DNA draws the accuracy that detects, and the device simple structure, convenient operation save a large amount of loaded down with trivial details steps, improve work efficiency greatly, and the cost of whole device is less, is fit for small-size experiment and uses.
Description
Technical Field
The utility model relates to a preprocessing device field especially relates to a preprocessing device for extraction of environmental sample DNA.
Background
With the development of society, people pay more and more attention to the protection and management of the environment, and researchers can regularly sample and investigate the environment and extract and research DNA in a sample.
However, if a sample extracted from an environment contains many impurities, particularly a liquid sample, and the DNA of the sample is directly studied, the obtained result is very inaccurate, so that the sample needs to be pretreated.
SUMMERY OF THE UTILITY MODEL
The utility model aims at solving the defects existing in the prior art and providing a pretreatment device for extracting the DNA of an environmental sample.
In order to achieve the above purpose, the utility model adopts the following technical scheme: a pretreatment device for extracting DNA of an environmental sample comprises a shell, wherein a feed inlet is arranged at the top end of the shell, the top end of the feed inlet is communicated with a top plate of the shell, two first support rails are fixedly arranged at the upper ends of the two side walls of the inner end of the shell, a large-particle sundry filter screen is arranged at the upper ends of the two first support rails, two second support rails are fixedly arranged at the lower ends of the two first support rails and are fixedly arranged on the two side walls of the shell, a small-particle sundry filter screen is arranged at the upper ends of the two second support rails, a funnel-shaped sundry filter screen is arranged at the lower end of the small-particle sundry filter screen and is fixedly connected to the inner wall of the shell, a support plate is arranged at the lower end of the funnel-shaped sundry filter screen and is fixedly connected to the inner wall, the utility model discloses a stirring chamber, including agitator, lower extreme outside, the fixed motor that is provided with in a lateral wall of agitator chamber by the lower extreme outside, the output fixedly connected with puddler of motor, the other end of puddler leads to the inner of agitator chamber and rotates and connect on the inner wall of agitator chamber, the pole of puddler is epaxial fixedly cup jointed the multiunit stirring leaf, the fixed discharge pipe that is equipped with is opened at agitator chamber bottom center, the low side of discharge pipe runs through the backup pad and fixedly is provided with flange, the flange lower extreme passes through bolt fixedly connected with greasy dirt filter, the bottom of casing is provided with storage container, storage container's the fixed carrying handle that is provided with in.
As a further description of the above technical solution:
the bottom end of the shell is fixedly provided with four bases.
As a further description of the above technical solution:
two support rails and two No. two support rails all through many screw fixed connection on the lateral wall of casing.
As a further description of the above technical solution:
and the pipe body of the discharge pipe is fixedly provided with an electromagnetic valve.
As a further description of the above technical solution:
a plurality of limiting blocks are fixedly arranged on the bottom plate of the shell, and the storage container is located in the limiting blocks.
As a further description of the above technical solution:
an upper box door and a lower box door are arranged on the front wall of the shell, and door handles are arranged on the upper box door and the lower box door.
The utility model discloses following beneficial effect has:
the utility model provides a preprocessing device for environmental sample DNA draws, the device are provided with multichannel filter equipment, can get rid of big or small graininess debris and the greasy dirt in the liquid environmental sample, guarantee that environmental sample DNA draws the accuracy that detects, and the device simple structure, convenient operation save a large amount of loaded down with trivial details steps, improve work efficiency greatly, and the cost of whole device is less, are fit for small-size experiment and use.
Drawings
FIG. 1 is a cross-sectional view of a pretreatment device for DNA extraction of environmental samples according to the present invention;
FIG. 2 is an enlarged view of FIG. 1-A in accordance with the present invention;
FIG. 3 is a front view of a pretreatment device for DNA extraction of environmental samples according to the present invention.
Illustration of the drawings:
1. a housing; 2. a feed inlet; 3. a first support rail; 4. a large-particle sundry filter screen; 5. a second support rail; 6. a small particle impurity filter screen; 7. a funnel-shaped middle collecting plate; 8. a support plate; 9. a stirring chamber; 10. a motor; 11. a stirring rod; 12. stirring blades; 13. a discharge pipe; 14. an electromagnetic valve; 15. a connecting flange; 16. an oil stain filter; 17. a limiting block; 18. a storage container; 19. a carrying handle; 20. an upper box door; 21. a lower box door; 22. a door handle; 23. a base; 24. and (4) screws.
Detailed Description
The technical solution in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention.
Referring to fig. 1-3, the present invention provides an embodiment: a pretreatment device for extracting DNA of an environmental sample comprises a shell 1, wherein a feed inlet 2 is arranged at the top end of the shell 1, a top cover can be arranged at the upper end of the feed inlet 2 to prevent the inner end of equipment from entering ash, the top end of the feed inlet 2 is communicated with a top plate of the shell 1, two first support rails 3 are fixedly arranged at the two side walls of the inner end of the shell 1 near the upper ends, large particle impurity filter screens 4 are arranged at the upper ends of the two first support rails 3, two second support rails 5 are fixedly arranged at the lower ends of the two first support rails 3 on the two side walls of the shell 1, small particle impurity filter screens 6 are arranged at the upper ends of the two second support rails 5, the large particle impurity filter screens 4 and the small particle impurity filter screens 6 are both arranged into square nets, frames are arranged at the side edges, a funnel-shaped central plate 7 is arranged at, the funnel-shaped collecting plate 7 is used for gathering sample liquid, the lower end of the funnel-shaped collecting plate 7 is provided with a supporting plate 8 fixedly connected to the inner wall of the shell 1, the upper end of the supporting plate 8 is fixedly provided with a stirring chamber 9, the stirring chamber 9 is used for stirring the sample liquid so as to facilitate oil-liquid separation and filtration, the bottom end of the funnel-shaped collecting plate 7 is communicated with the top plate of the stirring chamber 9, a motor 10 is fixedly arranged on the outer side of the lower end of one side wall of the stirring chamber 9, the output end of the motor 10 is fixedly connected with a stirring rod 11, the other end of the stirring rod 11 is communicated with the inner end of the stirring chamber 9 and is rotatably connected to the inner wall of the stirring chamber 9, a plurality of groups of stirring blades 12 are fixedly sleeved on the rod body of the stirring rod 11, the motor 10 drives the stirring rod 11 to rotate, the stirring rod 11 rotates to drive the plurality of groups of stirring blades 12 to rotate for, the lower end of the connecting flange 15 is fixedly connected with an oil stain filter 16 through bolts, the oil stain filter is used for filtering oil stains in sample liquid, the bottom end of the shell 1 is provided with a storage container 18 used for storing the processed sample liquid, and handles 19 are fixedly arranged at the outer ends of two side walls of the storage container 18.
The bottom mounting of casing 1 is provided with four bases 23, two support rails 3 and two support rails 5 all through many screws 24 fixed connection on the lateral wall of casing 1 for one number, the fixed solenoid valve 14 that is provided with on the pipe shaft of discharge pipe 13, be convenient for control the emission of agitator chamber 9 sample, fixed a plurality of stopper 17 that are provided with on the bottom plate of casing 1, storage container 18 is located a plurality of stopper 17, a plurality of stopper 17 can fix the bottom of storage container 18 spacing, prevent that storage container 18 from removing, be provided with chamber door 20 and lower chamber door 21 on the antetheca of casing 1, all be provided with door handle 22 on chamber door 20 and the lower chamber door 21, the setting of chamber door 20 and lower chamber door 21 can make things convenient for the washing of filter screen to change and take out of storage container 18.
The working principle is as follows: during the use, earlier pour liquid sample into in casing 1 from feed inlet 2, liquid sample is through large granule debris filter screen 4 and small granule debris filter screen 6, filter big or small granule debris in the liquid, then pass through funnel type collection medium board 7 and get into agitator chamber 9 the inner again, starter motor 10, motor 10 drives puddler 11 and rotates, puddler 11 rotates and drives multiunit stirring leaf 12 rotatory, stir the sample, break up the greasy dirt, so that the filtration of greasy dirt, open solenoid valve 14 after the stirring and arrange the sample from discharge pipe 13 to in greasy dirt filter 16, the sample is got rid of the greasy dirt through greasy dirt filter 16 and then falls into in storage container 18, open lower chamber door 21 at last and take out storage container 18.
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications and variations can be made in the embodiments or in part of the technical features of the embodiments without departing from the spirit and the scope of the invention.
Claims (6)
1. A pre-processing device for DNA extraction of environmental samples, comprising a housing (1), characterized in that: the top end of the shell (1) is provided with a feed inlet (2), the top end of the feed inlet (2) is communicated with a top plate of the shell (1), two first support rails (3) are fixedly arranged on the two side walls of the inner end of the shell (1) by the upper ends, large-particle impurity filter screens (4) are arranged on the upper ends of the two first support rails (3), two second support rails (5) are fixedly arranged on the two side walls of the shell (1) by the lower ends of the two first support rails (3), small-particle impurity filter screens (6) are arranged on the upper ends of the two second support rails (5), a funnel-shaped middle collecting plate (7) is arranged at the lower end of each small-particle impurity filter screen (6) and fixedly connected to the inner wall of the shell (1), a support plate (8) is arranged at the lower end of the funnel-shaped middle collecting plate (7) and fixedly connected to the inner wall of the shell (1), and a, the bottom end of the funnel-shaped centralized plate (7) is communicated with the top plate of the stirring chamber (9), a motor (10) is fixedly arranged on the outer side of one side wall of the stirring chamber (9) close to the lower end, the output end of the motor (10) is fixedly connected with a stirring rod (11), the other end of the stirring rod (11) is communicated with the inner end of the stirring chamber (9) and is rotatably connected on the inner wall of the stirring chamber (9), a plurality of groups of stirring blades (12) are fixedly sleeved on the body of the stirring rod (11), a discharge pipe (13) is fixedly arranged at the center of the bottom end of the stirring chamber (9), the lower end of the discharge pipe (13) penetrates through the support plate (8) and is fixedly provided with a connecting flange (15), the lower end of the connecting flange (15) is fixedly connected with an oil stain filter (16) through bolts, the bottom end of the shell (1) is provided with a storage container (18), and the outer ends of the two side walls of the storage container (18) are fixedly provided with handles (19).
2. The pretreatment device for DNA extraction of environmental samples according to claim 1, characterized in that: the bottom end of the shell (1) is fixedly provided with four bases (23).
3. The pretreatment device for DNA extraction of environmental samples according to claim 1, characterized in that: the two first support rails (3) and the two second support rails (5) are fixedly connected to the side wall of the shell (1) through a plurality of screws (24).
4. The pretreatment device for DNA extraction of environmental samples according to claim 1, characterized in that: an electromagnetic valve (14) is fixedly arranged on the tube body of the discharge tube (13).
5. The pretreatment device for DNA extraction of environmental samples according to claim 1, characterized in that: a plurality of limiting blocks (17) are fixedly arranged on a bottom plate of the shell (1), and the storage container (18) is located in the limiting blocks (17).
6. The pretreatment device for DNA extraction of environmental samples according to claim 1, characterized in that: an upper box door (20) and a lower box door (21) are arranged on the front wall of the shell (1), and door handles (22) are arranged on the upper box door (20) and the lower box door (21).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020114665.6U CN211847897U (en) | 2020-01-19 | 2020-01-19 | Pretreatment device for extracting DNA (deoxyribonucleic acid) of environmental sample |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020114665.6U CN211847897U (en) | 2020-01-19 | 2020-01-19 | Pretreatment device for extracting DNA (deoxyribonucleic acid) of environmental sample |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN211847897U true CN211847897U (en) | 2020-11-03 |
Family
ID=73232906
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202020114665.6U Expired - Fee Related CN211847897U (en) | 2020-01-19 | 2020-01-19 | Pretreatment device for extracting DNA (deoxyribonucleic acid) of environmental sample |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN211847897U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113773947A (en) * | 2021-11-11 | 2021-12-10 | 中国矿业大学(北京) | Rapid extraction equipment for organic matters in mineral slurry |
-
2020
- 2020-01-19 CN CN202020114665.6U patent/CN211847897U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113773947A (en) * | 2021-11-11 | 2021-12-10 | 中国矿业大学(北京) | Rapid extraction equipment for organic matters in mineral slurry |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN211847897U (en) | Pretreatment device for extracting DNA (deoxyribonucleic acid) of environmental sample | |
| CN217651159U (en) | Walnut oil processing filtration equipment | |
| CN216629753U (en) | Ambroxol hydrochloride active carbon filter equipment | |
| CN214809097U (en) | Liquid collecting device for plant extraction experiment | |
| CN214019416U (en) | Wort filter equipment | |
| CN221086347U (en) | Quick screening plant is used in soil detection | |
| CN117660774B (en) | Device for extracting gold from waste electronic element | |
| CN221157674U (en) | Pollen collecting device | |
| CN220899632U (en) | Water sample filtering structure of rapid detection card for heavy metal cadmium in vegetables | |
| CN212083012U (en) | Sample preprocessing device for soil detection | |
| CN220922854U (en) | Concrete stirring dust collector | |
| CN220812420U (en) | Stem cell filtering separator | |
| CN220834302U (en) | A high-efficient filter equipment for in honey processing | |
| CN221014873U (en) | Centrifugal device is used in sesame oil processing | |
| CN217829218U (en) | Multifunctional water paint wastewater treatment device | |
| CN213985895U (en) | Sample processing device for food safety monitoring | |
| CN217188120U (en) | Impurity filtering device for processing infant moisturizing cream | |
| CN210065356U (en) | Solid-liquid separation device for environmental improvement | |
| CN217829058U (en) | Three-phase separator for sewage treatment | |
| CN221038252U (en) | Soil pesticide residue extraction element | |
| CN218475352U (en) | Root of kudzu vine extraction equipment is used in root of kudzu vine powder production | |
| CN219129532U (en) | Broken material separator for lead-acid battery | |
| CN218458702U (en) | Extraction device for separating biological reagents | |
| CN215876651U (en) | Dust remover for sludge treatment tail gas | |
| CN216073505U (en) | Oily sludge treatment system |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20201103 |