CN217948088U - Stem cell exosome separation and extraction device - Google Patents

Abstract

The utility model relates to a cell is from drawing technical field, discloses a stem cell exosome separation extraction element, which comprises a tank body, the top threaded connection of the jar body has the top cap, the upper surface center of top cap goes out the first motor of fixedly connected with, the output fixedly connected with bull stick of first motor, the outer wall fixedly connected with puddler of bull stick, the middle part outer wall fixedly connected with slider of the jar body, the outer wall sliding connection of slider has the spout. The utility model discloses in, the output through first motor drives the bull stick and rotates, and the bull stick drives the puddler and rotates, and the second motor drives the pivot and rotates, and the pivot drives a jar body and rotates, has reached through this kind of design, carries out the centrifugation when the stirring and rotates, has improved work efficiency, has reached the effect that can conveniently take out a jar body more through the design of slider, spout and shell, has reached the effect of convenient clearance jar internal portion simultaneously.

Description

A device for separating and extracting stem cell exosomes

technical field

The utility model relates to the technical field of cell separation and extraction, in particular to a stem cell exosome separation and extraction device.

Background technique

Exosomes are an important part of intercellular material and information transfer, which can transfer biomolecules such as lipids, carbohydrates, proteins, mRNA, miRNA, and DNA from one cell to another, thereby exchanging genetic information, reprogramming Host cells, for intercellular communication, are carriers for exchanging information and exchanging substances between cells. In addition, studies have confirmed that exosomes usually have the biological characteristics of their source cells, such as stem cell exosomes, which also have the ability to differentiate and regenerate. , low immunogenicity, and high survival rate after transplantation.

The Chinese patent with the application number CN202121695712.1 discloses a device for separating and extracting exosomes from stem cells, which includes a housing. device, the stirring device includes a bracket, the top of the bracket is vertically provided with a circular through hole, the top of the extraction barrel is provided with a circular hole and the circular hole is symmetrical with the circular through hole on the bracket, and the bottom of the rotating shaft is fixedly installed with a first The rotating column and the outer wall of the first rotating column are fixedly equipped with first rotating vanes. In the utility model, the stem cells and exosomes injected into the extraction barrel are rapidly stirred, and the different densities of the stem cells and exosomes can be diluted and separated when being stirred, and then the stirred stem cells and exosomes are As the separation box flows downward, the separation block inside the separation box can separate the stem cells flowing downward. This patent only extracts stem cell exosomes by stirring, and the work efficiency is relatively low.

Utility model content

The purpose of the utility model is to solve the shortcomings in the prior art, and propose a stem cell exosome separation and extraction device.

In order to achieve the above purpose, the utility model adopts the following technical scheme: a stem cell exosome separation and extraction device, including a tank body, the top of the tank body is threadedly connected with a top cover, and the center of the upper surface of the top cover is fixed A first motor is connected, the output end of the first motor is fixedly connected with a rotating rod, the outer wall of the rotating rod is fixedly connected with a stirring rod, the middle outer wall of the tank body is fixedly connected with a slider, and the outer wall of the slider is fixedly connected with a slider. The outer wall is slidably connected with a chute, the chute is set inside the shell, the top of the rotating shaft is fixedly connected to the center of the lower surface of the tank body, and the bottom end of the rotating shaft is fixedly connected to the output end of the second motor. The second motor is fixedly connected in the placement groove, and the placement groove is opened at the center of the upper surface of the placement frame.

As a further description of the above technical solution:

The upper surface of the placing frame is fixedly connected with a fixing plate.

As a further description of the above technical solution:

A discharge port is opened on the outer wall of the bottom end of the shell, and the discharge port is opened to the inside of the tank body.

As a further description of the above technical solution:

One end of the connecting pipe is fixedly connected to the inner wall of the discharge port, and one end of the connecting pipe is connected to the inside of the tank body, and the first sieve plate is fixedly connected to the inner wall of the connecting pipe.

As a further description of the above technical solution:

The other end of the connecting pipe is fixedly connected to the input end of the valve, the output end of the valve is fixedly connected to the discharge pipe, and the inner wall of the discharge pipe is fixedly connected to the second sieve plate.

As a further description of the above technical solution:

A top shell is connected to the upper surface of the shell.

As a further description of the above technical solution:

A sealing pipe is fixedly connected to one side of the upper surface of the top shell, a feeding pipe is fixedly connected to the inner wall of the sealing pipe, and a sealing cover is threadedly connected to the top end of the feeding pipe.

As a further description of the above technical solution:

The feed pipe is connected to the inside of the tank.

The utility model has the following beneficial effects:

1. In the utility model, firstly, the output end of the first motor drives the rotating rod to rotate, the rotating rod drives the stirring rod to rotate, the second motor drives the rotating shaft to rotate, and the rotating shaft drives the tank body to rotate. This design achieves , Centrifugal rotation is carried out while stirring, which improves the work efficiency. Through the design of the slider, chute and shell, the effect of taking out the tank body can be more conveniently achieved, and at the same time, the effect of cleaning the inside of the tank body is conveniently achieved.

2. In this utility model, a more stable working environment is provided for the device through the design of the placing frame and the fixing plate, and the design of the connecting pipe, the first sieve plate, the valve, the discharge pipe and the second sieve plate achieves the The device has a better filtering effect, making the extracted exosomes more pure, and through the design of the sealing tube and the sealing cover, the device has a better sealing effect and reduces the contamination of raw materials.

Description of drawings

Fig. 1 is the front view of a kind of stem cell exosome separation and extraction device proposed by the utility model;

Fig. 2 is a schematic diagram of a front-view stereoscopic structure of a device for separating and extracting stem cell exosomes proposed by the present invention;

Fig. 3 is an exploded view of a stem cell exosome separation and extraction device proposed by the utility model;

Fig. 4 is a structural schematic diagram of the valve part of a device for separating and extracting stem cell exosomes proposed by the present invention.

illustration:

1. Tank body; 2. Top cover; 3. First motor; 4. Rotating rod; 5. Stirring rod; 6. Slider; 7. Chute; 8. Shell; 9. Rotating shaft; 10. Second motor; 11. Placement slot; 12. Placement frame; 13. Fixed plate; 14. Discharge port; 15. Connecting pipe; 16. First sieve plate; 17. Valve; 18. Discharge pipe; 19. Second sieve plate; 20. Top shell; 21. Sealing tube; 22. Feeding tube; 23. Sealing cover.

detailed description

The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. example. Based on the embodiments of the present utility model, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the scope of protection of the present utility model.

In the description of the present utility model, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer" The orientation or positional relationship indicated by etc. is based on the orientation or positional relationship shown in the drawings, which is only for the convenience of describing the utility model and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, use a specific Therefore, it cannot be construed as a limitation on the present invention; the terms "first", "second", and "third" are only used for descriptive purposes, and cannot be interpreted as indicating or implying relative importance. In addition, Unless otherwise clearly specified and limited, the terms "installation", "connection" and "connection" should be interpreted in a broad sense, for example, it may be a fixed connection, a detachable connection, or an integral connection; it may be a mechanical connection, It can also be an electrical connection; it can be a direct connection, or an indirect connection through an intermediary, or an internal communication between two components. Those of ordinary skill in the art can understand the specific meanings of the above terms in the present utility model in specific situations.

Referring to Figures 1-4, an embodiment provided by the present invention: a device for separating and extracting stem cell exosomes, comprising a tank body 1, the top of the tank body 1 is threadedly connected with a top cover 2, and the center of the upper surface of the top cover 2 is The first motor 3 is fixedly connected to the outlet, the output end of the first motor 3 is fixedly connected to the rotating rod 4, the outer wall of the rotating rod 4 is fixedly connected to the stirring rod 5, and the outer wall of the middle part of the tank body 1 is fixedly connected to the slider 6. The outer wall of 6 is slidably connected with a chute 7, and the chute 7 is set inside the shell 8, and the center of the lower surface of the tank body 1 is fixedly connected with the top of the rotating shaft 9, and the bottom end of the rotating shaft 9 is fixedly connected with the output end of the second motor 10 , the second motor 10 is fixedly connected in the placement groove 11, and the placement groove 11 is provided at the center of the upper surface of the placement frame 12. Through the above design, the centrifugal rotation is achieved while stirring, and the work efficiency is improved.

The upper surface of the placement frame 12 is fixedly connected with a fixed plate 13. Through this design, a good support effect is provided for the tank body 1. The outer wall of the bottom end of the shell 8 is provided with a discharge port 14, and the discharge port 14 is opened to Inside the tank body 1, the inner wall of the discharge port 14 is fixedly connected with one end of the connecting pipe 15, and one end of the connecting pipe 15 is connected to the inside of the tank body 1, and the inner wall of the connecting pipe 15 is fixedly connected with the first sieve plate 16, and the connecting pipe 15 The other end is fixedly connected to the input end of the valve 17, the output end of the valve 17 is fixedly connected to the discharge pipe 18, and the inner wall of the discharge pipe 18 is fixedly connected to the second sieve plate 19. Through this design, the device has a multi-layer The effect of filtering makes the extracted exosomes more pure. The top shell 20 is connected to the upper surface of the shell 8, and the upper surface side of the top shell 20 is fixedly connected with a sealing tube 21, and the inner wall of the sealing tube 21 is fixedly connected with an inlet The feed pipe 22 and the top end of the feed pipe 22 are threadedly connected with a sealing cover 23, which achieves a better sealing effect and reduces the contamination of raw materials. The feed pipe 22 is connected to the inside of the tank body 1.

Working principle: When it is necessary to extract exocrine bodies of stem cells, the sealing cover 23 is first removed by rotating, the raw material is injected into the tank body 1 through the feeding tube 22, and the sealing cover 23 is connected to the feeding tube 22 by rotating. Connect, then start the first motor 3 and the second motor 10, the output end of the first motor 3 drives the rotating rod 4 to rotate, the stirring rod 5 rotates with the rotation of the rotating rod 4, and the output end of the second motor 10 synchronously drives The rotating shaft 9 rotates, and the tank body 1 rotates with the rotation of the rotating shaft 9. Through the above design, the centrifuge rotation is carried out while stirring, which improves the work efficiency. After the extraction is completed, the valve 17 is opened, and the extraction in the tank body 1 After the stem cell exosomes are collected, they enter the connecting pipe 15 through the first sieve plate 16, and then flow out through the second sieve plate 19. Through this design, a multi-layer filtration effect is achieved, so that the extracted stem cells are exosomes Secretion is more pure. When the tank 1 needs to be cleaned, the top shell 20 is first removed, the tank 1 is taken out from the shell 8, the top cover 2 is removed by rotating, and then the inside of the tank 1 can be cleaned.

Finally, it should be noted that: the above is only a preferred embodiment of the utility model, and is not intended to limit the utility model, although the utility model has been described in detail with reference to the foregoing embodiments, for those skilled in the art , it can still modify the technical solutions recorded in the foregoing embodiments, or perform equivalent replacements on some of the technical features. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present utility model, All should be included within the protection scope of the present utility model.

Claims (8)

1. The utility model provides a stem cell exosome separation extraction element, includes jar body (1), its characterized in that: the utility model discloses a storage tank, including the jar body, the top end threaded connection of the jar body (1) has top cap (2), the upper surface center of top cap (2) goes out the first motor of fixedly connected with (3), the output end fixedly connected with bull stick (4) of first motor (3), the outer wall fixedly connected with puddler (5) of bull stick (4), the middle part outer wall fixedly connected with slider (6) of the jar body (1), the outer wall sliding connection of slider (6) has spout (7), spout (7) are seted up inside shell (8), the top of fixedly connected with pivot (9) is located at the lower surface center of the jar body (1), the bottom fixed connection of pivot (9) is at the output of second motor (10), second motor (10) fixed connection is in standing groove (11), the upper surface center department at rack (12) is seted up in standing groove (11).

2. The device for separating and extracting the exosomes of the stem cells according to claim 1, which is characterized in that: the upper surface of the placing rack (12) is fixedly connected with a fixing plate (13).

3. The device for separating and extracting the exosomes of the stem cells according to claim 1, which is characterized in that: the outer wall of the bottom end of the shell (8) is provided with a discharge hole (14), and the discharge hole (14) is formed in the tank body (1).

4. The device for separating and extracting the exosomes of the stem cells according to claim 3, characterized in that: the one end of the inner wall fixedly connected with connecting pipe (15) of discharge gate (14), the one end of connecting pipe (15) is connected to the jar body (1) inside, the first sieve of inner wall fixedly connected with (16) of connecting pipe (15).

5. The device for separating and extracting the exosomes of the stem cells according to claim 4, characterized in that: the other end fixedly connected with valve (17) of connecting pipe (15) the input, the output fixedly connected with discharging pipe (18) of valve (17), the inner wall fixedly connected with second sieve (19) of discharging pipe (18).

6. The device for separating and extracting the exosomes of the stem cells according to claim 1, which is characterized in that: the upper surface of the shell (8) is connected with a top shell (20).

7. The device for separating and extracting the exosomes of the stem cells according to claim 6, which is characterized in that: one side of the upper surface of the top shell (20) is fixedly connected with a sealing pipe (21), the inner wall of the sealing pipe (21) is fixedly connected with an inlet pipe (22), and the top end of the inlet pipe (22) is in threaded connection with a sealing cover (23).

8. The device for separating and extracting the exosomes of the stem cells according to claim 7, which is characterized in that: the feeding pipe (22) is connected to the inside of the tank body (1).

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