CN217948088U - Stem cell exosome separation and extraction device - Google Patents
Stem cell exosome separation and extraction device Download PDFInfo
- Publication number
- CN217948088U CN217948088U CN202221321861.6U CN202221321861U CN217948088U CN 217948088 U CN217948088 U CN 217948088U CN 202221321861 U CN202221321861 U CN 202221321861U CN 217948088 U CN217948088 U CN 217948088U
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- China
- Prior art keywords
- fixedly connected
- jar body
- motor
- wall
- exosomes
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- 210000000130 stem cell Anatomy 0.000 title claims abstract description 28
- 210000001808 exosome Anatomy 0.000 title claims abstract description 27
- 238000000926 separation method Methods 0.000 title claims abstract description 15
- 238000000605 extraction Methods 0.000 title claims abstract description 13
- 244000309464 bull Species 0.000 claims abstract description 14
- 238000007599 discharging Methods 0.000 claims description 7
- 241000237983 Trochidae Species 0.000 claims description 6
- 238000007789 sealing Methods 0.000 claims description 5
- 230000000694 effects Effects 0.000 abstract description 9
- 238000003756 stirring Methods 0.000 abstract description 7
- 210000004027 cell Anatomy 0.000 abstract description 5
- 238000005119 centrifugation Methods 0.000 abstract description 3
- 239000000243 solution Substances 0.000 description 8
- 239000002994 raw material Substances 0.000 description 3
- 230000035992 intercellular communication Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 108020004414 DNA Proteins 0.000 description 1
- 241000463219 Epitheca Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 108020004999 messenger RNA Proteins 0.000 description 1
- 108091070501 miRNA Proteins 0.000 description 1
- 239000002679 microRNA Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000002365 multiple layer Substances 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000008672 reprogramming Effects 0.000 description 1
- 230000008093 supporting effect Effects 0.000 description 1
Images
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- Extraction Or Liquid Replacement (AREA)
Abstract
The utility model relates to a cell is from drawing technical field, discloses a stem cell exosome separation extraction element, which comprises a tank body, the top threaded connection of the jar body has the top cap, the upper surface center of top cap goes out the first motor of fixedly connected with, the output fixedly connected with bull stick of first motor, the outer wall fixedly connected with puddler of bull stick, the middle part outer wall fixedly connected with slider of the jar body, the outer wall sliding connection of slider has the spout. The utility model discloses in, the output through first motor drives the bull stick and rotates, and the bull stick drives the puddler and rotates, and the second motor drives the pivot and rotates, and the pivot drives a jar body and rotates, has reached through this kind of design, carries out the centrifugation when the stirring and rotates, has improved work efficiency, has reached the effect that can conveniently take out a jar body more through the design of slider, spout and shell, has reached the effect of convenient clearance jar internal portion simultaneously.
Description
Technical Field
The utility model relates to a cell is from drawing technical field, especially relates to a stem cell exosome separation extraction element.
Background
The exosome is an important ring participating in intercellular substance and information transmission, can transmit biomolecules such as lipids, carbohydrates, proteins, mRNA, miRNA and DNA from one cell to another cell, thereby exchanging genetic information, reprogramming Cheng Suzhu cells, and performing intercellular communication, and is a carrier for intercellular communication information and exchange of substances.
Chinese patent with application number CN202121695712.1 discloses a stem cell exosome separation extraction element, which comprises an outer shell, the circular port has been seted up to the bottom of shell, and the inside of shell is pegged graft and has is drawed the bucket, and the inside of shell is provided with agitating unit, and agitating unit includes the support, and circular through-hole has vertically been seted up at the top of support, and it is symmetrical to set up circular through-hole on round hole and the support to draw the top of bucket, and the bottom fixed mounting of pivot has first column spinner, and equal fixed mounting has first rotatory leaf on the outer wall of first column spinner. The utility model discloses in, the stem cell and the exosome of the inside injection of extraction bucket are by rapid mixing, and the different density of stem cell and exosome can dilute and separate when being stirred, then the stem cell and the exosome with the stirring trickling downwards along with the separation box, and the inside separation piece of separation box can separate the stem cell of downward flow, and this patent only draws the stem cell exosome through the stirring, and work efficiency is comparatively low.
SUMMERY OF THE UTILITY MODEL
The utility model aims at solving the defects existing in the prior art and providing a stem cell exosome separation and extraction device.
In order to achieve the above purpose, the utility model adopts the following technical scheme: the utility model provides a stem cell exosome separation extraction element, includes a jar body, the top end threaded connection of the jar body has the top cap, the first motor of fixedly connected with is gone out at the upper surface center of top cap, the output fixedly connected with bull stick of first motor, the outer wall fixedly connected with puddler of bull stick, the middle part outer wall fixedly connected with slider of the jar body, the outer wall sliding connection of slider has the spout, the spout is seted up inside the shell, the top of the fixedly connected with pivot is located at the lower surface center of the jar body, the bottom fixed connection of pivot is at the output of second motor, second motor fixed connection is in the standing groove, the upper surface center department at the rack is seted up to the standing groove.
As a further description of the above technical solution:
the upper surface of the placing frame is fixedly connected with a fixing plate.
As a further description of the above technical solution:
the discharge gate has been seted up to the bottom outer wall of shell, the discharge gate is seted up to jar internal portion.
As a further description of the above technical solution:
the inner wall fixedly connected with one end of connecting pipe of discharge gate, the one end of connecting pipe is connected to a jar internal portion, the first sieve of inner wall fixedly connected with of connecting pipe.
As a further description of the above technical solution:
the other end of the connecting pipe is fixedly connected with an input end of a valve, an output end of the valve is fixedly connected with a discharging pipe, and the inner wall of the discharging pipe is fixedly connected with a second sieve plate.
As a further description of the above technical solution:
the upper surface of the shell is connected with a top shell.
As a further description of the above technical solution:
the upper surface one side fixedly connected with sealed tube of epitheca, the inner wall fixedly connected with inlet pipe of sealed tube, the top threaded connection of inlet pipe has sealed lid.
As a further description of the above technical solution:
the inlet pipe is connected to the inside of the jar body.
The utility model discloses following beneficial effect has:
1. the utility model discloses in, at first, the output through first motor drives the bull stick and rotates, and the bull stick drives the puddler and rotates, and the second motor drives the pivot and rotates, and the pivot drives a jar body and rotates, has reached through this kind of design, carries out the centrifugation when the stirring and rotates, has improved work efficiency, has reached the effect that can conveniently take out a jar body more through the design of slider, spout and shell, has reached the effect of conveniently clearing up a jar internal portion simultaneously.
2. The utility model discloses in, design through rack and fixed plate provides more stable operational environment for this device, has reached through the design of connecting pipe, first sieve, valve, discharging pipe and second sieve and has made this device possess better filter effect, makes the exosome of having extracted more add purer, makes this device possess better sealed effect through the design of sealed pipe with sealed lid, has reduced the condition of raw materials pollution.
Drawings
Fig. 1 is a front view of a stem cell exosome separation and extraction device provided by the present invention;
fig. 2 is a schematic view of a front perspective three-dimensional structure of a stem cell exosome separation and extraction device provided by the present invention;
fig. 3 is an exploded view of a stem cell exosome separation and extraction device provided by the present invention;
fig. 4 is a schematic structural view of a valve portion of the device for separating and extracting exosomes from stem cells according to the present invention.
Illustration of the drawings:
1. a tank body; 2. a top cover; 3. a first motor; 4. a rotating rod; 5. a stirring rod; 6. a slider; 7. a chute; 8. a housing; 9. a rotating shaft; 10. a second motor; 11. a placement groove; 12. placing a rack; 13. a fixing plate; 14. a discharge port; 15. a connecting pipe; 16. a first screen deck; 17. a valve; 18. a discharge pipe; 19. a second screen deck; 20. a top shell; 21. sealing the tube; 22. a feed pipe; 23. and (7) sealing the cover.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention; the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance, and furthermore, unless otherwise explicitly stated or limited, the terms "mounted," "connected," and "connected" are to be construed broadly and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art.
Referring to fig. 1-4, the present invention provides an embodiment: the utility model provides a stem cell exosome separation extraction element, which comprises a tank body 1, the top end threaded connection of the jar body 1 has top cap 2, the first motor 3 of fixedly connected with is gone out at the upper surface center of top cap 2, the output end fixedly connected with bull stick 4 of first motor 3, the outer wall fixedly connected with puddler 5 of bull stick 4, the middle part outer wall fixedly connected with slider 6 of the jar body 1, slider 6's outer wall sliding connection has spout 7, spout 7 is seted up inside shell 8, the top of fixedly connected with pivot 9 is located at the lower surface center of the jar body 1, the bottom fixed connection of pivot 9 is at the output of second motor 10, 10 fixed connection of second motor are in standing groove 11, the upper surface center department at rack 12 is seted up to standing groove 11, reached through above design and carried out the centrifugation when the stirring and rotate, and work efficiency is improved.
The last fixed surface of rack 12 is connected with fixed plate 13, it provides a good supporting effect for jar body 1 to have reached through this kind of design, discharge gate 14 has been seted up to the bottom outer wall of shell 8, discharge gate 14 is seted up to jar body 1 inside, the one end of the inner wall fixedly connected with connecting pipe 15 of discharge gate 14, the one end of connecting pipe 15 is connected to jar body 1 inside, the first sieve 16 of the inner wall fixedly connected with of connecting pipe 15, the input of the other end fixedly connected with valve 17 of connecting pipe 15, the output fixedly connected with discharging pipe 18 of valve 17, the inner wall fixedly connected with second sieve 19 of discharging pipe 18, make this device possess the effect of multiple-layer filtering through this kind of design, the exosome that has made and extracted out adds more, the upper surface of shell 8 is connected with top shell 20, upper surface one side fixedly connected with sealed tube 21 of top shell 20, the inner wall fixedly connected with inlet pipe 22 of sealed tube 21, the top threaded connection of inlet pipe 22 has sealed lid 23, a better sealed effect has been reached through this kind of design, the condition of raw materials pollution has been reduced, inlet pipe 22 is connected to jar body 1 inside.
The working principle is as follows: when the stem cell exocrine body needs to be extracted, at first take off sealed lid 23 through the pivoted mode, inject the raw materials into jar 1 through inlet pipe 22 in, seal lid 23 is connected with inlet pipe 22 through the pivoted mode, then start first motor 3 and second motor 10, the output of first motor 3 drives bull stick 4 and rotates, puddler 5 rotates along with the rotation of bull stick 4, the output of second motor 10 drives pivot 9 in step and rotates, jar 1 rotates along with the rotation of pivot 9, through above design has reached and has carried out centrifugal rotation when the stirring, the work efficiency is improved, after finishing drawing, open valve 17, the stem cell exocrine body after finishing drawing in jar 1, enter into connecting pipe 15 through first sieve 16, then flow out through second sieve 19, a kind of multilayer filter's effect has been reached through this kind of design, make the stem cell exocrine body of extracting more add purer, when the jar body 1 needs to be cleaned, at first take off top cap 20, take out jar body 1 from the shell 8 in, then take out jar body 2 with the pivoted mode, can take off the inside of jar body and wash through the top cap 1.
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications and variations can be made in the embodiments or in part of the technical features of the embodiments without departing from the spirit and the scope of the invention.
Claims (8)
1. The utility model provides a stem cell exosome separation extraction element, includes jar body (1), its characterized in that: the utility model discloses a storage tank, including the jar body, the top end threaded connection of the jar body (1) has top cap (2), the upper surface center of top cap (2) goes out the first motor of fixedly connected with (3), the output end fixedly connected with bull stick (4) of first motor (3), the outer wall fixedly connected with puddler (5) of bull stick (4), the middle part outer wall fixedly connected with slider (6) of the jar body (1), the outer wall sliding connection of slider (6) has spout (7), spout (7) are seted up inside shell (8), the top of fixedly connected with pivot (9) is located at the lower surface center of the jar body (1), the bottom fixed connection of pivot (9) is at the output of second motor (10), second motor (10) fixed connection is in standing groove (11), the upper surface center department at rack (12) is seted up in standing groove (11).
2. The device for separating and extracting the exosomes of the stem cells according to claim 1, which is characterized in that: the upper surface of the placing rack (12) is fixedly connected with a fixing plate (13).
3. The device for separating and extracting the exosomes of the stem cells according to claim 1, which is characterized in that: the outer wall of the bottom end of the shell (8) is provided with a discharge hole (14), and the discharge hole (14) is formed in the tank body (1).
4. The device for separating and extracting the exosomes of the stem cells according to claim 3, characterized in that: the one end of the inner wall fixedly connected with connecting pipe (15) of discharge gate (14), the one end of connecting pipe (15) is connected to the jar body (1) inside, the first sieve of inner wall fixedly connected with (16) of connecting pipe (15).
5. The device for separating and extracting the exosomes of the stem cells according to claim 4, characterized in that: the other end fixedly connected with valve (17) of connecting pipe (15) the input, the output fixedly connected with discharging pipe (18) of valve (17), the inner wall fixedly connected with second sieve (19) of discharging pipe (18).
6. The device for separating and extracting the exosomes of the stem cells according to claim 1, which is characterized in that: the upper surface of the shell (8) is connected with a top shell (20).
7. The device for separating and extracting the exosomes of the stem cells according to claim 6, which is characterized in that: one side of the upper surface of the top shell (20) is fixedly connected with a sealing pipe (21), the inner wall of the sealing pipe (21) is fixedly connected with an inlet pipe (22), and the top end of the inlet pipe (22) is in threaded connection with a sealing cover (23).
8. The device for separating and extracting the exosomes of the stem cells according to claim 7, which is characterized in that: the feeding pipe (22) is connected to the inside of the tank body (1).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221321861.6U CN217948088U (en) | 2022-05-30 | 2022-05-30 | Stem cell exosome separation and extraction device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221321861.6U CN217948088U (en) | 2022-05-30 | 2022-05-30 | Stem cell exosome separation and extraction device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN217948088U true CN217948088U (en) | 2022-12-02 |
Family
ID=84216904
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202221321861.6U Expired - Fee Related CN217948088U (en) | 2022-05-30 | 2022-05-30 | Stem cell exosome separation and extraction device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN217948088U (en) |
-
2022
- 2022-05-30 CN CN202221321861.6U patent/CN217948088U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20221202 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |