CN215012696U - A small-size aseptic breeding device of shrimp for laboratory - Google Patents
A small-size aseptic breeding device of shrimp for laboratory Download PDFInfo
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- CN215012696U CN215012696U CN202023034540.1U CN202023034540U CN215012696U CN 215012696 U CN215012696 U CN 215012696U CN 202023034540 U CN202023034540 U CN 202023034540U CN 215012696 U CN215012696 U CN 215012696U
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- blake bottle
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Abstract
The utility model discloses an aseptic breeding device of shrimp for laboratory, characteristics are including the blake bottle that is used for breeding the shrimp, the upper end of blake bottle is provided with the bottle lid, be provided with air vent and intake pipe on the bottle lid, air vent department covers has the filter membrane that the one deck is used for preventing microorganism entering blake bottle, the one end of intake pipe is stretched into in the blake bottle lower part and its other end and is connected with the glass return bend, the mouth of pipe department of glass return bend is provided with ventilation regulating switch, ventilation regulating switch passes through the breather pump of breather hose connection, the last setting of breather hose is used for preventing microorganism entering the syringe formula filter membrane filter in the blake bottle, the advantage is that can sterilize completely, and easy and simple to handle.
Description
Technical Field
The utility model belongs to the technical field of the aquaculture technique and specifically relates to a shrimp breeding device for laboratory is related to.
Background
Litopenaeus vannamei (A) and (B)Litopenaeus vannamei) The prawn breeding method has the advantages of wide temperature adaptation range (6-39 ℃), wide salinity adaptation range (0.5-40), fast growth, strong disease resistance, delicious meat quality, high meat yield and the like, and becomes one of the most main prawn breeding varieties in China. However, with the industrial and intensive high-density culture of litopenaeus vannamei and the abuse of drugs such as antibiotics, the water quality deterioration and the change of microbial communities are easily caused, and further, the outbreak of diseases of the litopenaeus vannamei is a major bottleneck for the development of the litopenaeus vannamei culture industry. Therefore, the research on the disease generating process of the prawns and the probiotic mechanism thereof has important significance for realizing the high-efficiency green development of the prawns industry.
A set of culture equipment capable of keeping prawns in the best health state and controlling culture conditions is a basic condition for developing scientific research by using the culture equipment, but at present, an open culture mode is mostly adopted in a laboratory prawn culture test, and the prawn culture test is easily influenced by factors such as climate, impurities, water quality, external organisms and the like in the culture process, so that a culture system is not stable enough, and the problems of large experimental data deviation, unreliable data and the like are caused. In addition, most of the existing prawn culture experimental systems are carried out in dozens of to hundreds of liters of water, and pollution discharge and water replacement are required to be carried out regularly during culture, so that the workload is high, and higher requirements are also met for experimental sites. Therefore, the development of a set of small and medium-sized laboratory culture equipment suitable for prawn culture has great significance for further research on basic research related to prawns.
Disclosure of Invention
The utility model aims to solve the technical problem that a but prawn breeding device for laboratory of complete sterilization is provided.
The utility model provides a technical scheme that above-mentioned technical problem adopted does: the utility model provides a shrimp breeding device for laboratory, is including the blake bottle that is used for breeding the shrimp, the upper end of blake bottle be provided with the bottle lid, the bottle lid on be provided with air vent and intake pipe, air vent department cover and be used for preventing that the microorganism gets into the blake bottle in the filter membrane, the one end of intake pipe stretch into the blake bottle in lower part and its other end be connected with the glass return bend, the mouth of pipe department of glass return bend be provided with ventilation regulating switch, ventilation regulating switch pass through air hose and connect the breather pump, air hose on set up and be used for preventing that the microorganism gets into the blake bottle in the cylinder filter membrane filter.
The pore diameter of the filter membrane is 0.22 μm.
Compared with the prior art, the utility model has the advantages of: the patent of the utility model relates to a shrimp breeding device for laboratory, the device material is easy, with low costs, practice thrift breed space, equipment operation stable, can also realize the aseptic culture of shrimp simultaneously, is particularly useful for the research of shrimp intestinal microbiology and probiotic benefit mechanism etc. thereof.
Drawings
Fig. 1 is a schematic structural view of the sterile prawn culture device used in the laboratory.
Detailed Description
The invention is described in further detail below with reference to the accompanying examples.
DETAILED DESCRIPTION OF EMBODIMENT (S) OF INVENTION
The utility model provides a small-size aseptic breeding device of shrimp for laboratory, as shown in figure 1, including the blake bottle 1 that is used for breeding the shrimp, the upper end of blake bottle 1 is provided with bottle lid 2, be provided with air vent 3 and intake pipe 4 on the bottle lid 2, air vent 3 department covers has the filter membrane 5 that the one deck is used for preventing microorganism to get into blake bottle 1, the one end of intake pipe 4 stretches into in blake bottle 1 lower part and its other end and is connected with glass return bend 6, the mouth of pipe department of glass return bend 6 is provided with ventilation regulating switch 7, ventilation regulating switch 7 passes through ventilation hose 8 and connects breather pump 9, the last setting of ventilation hose 8 is used for preventing microorganism to get into syringe formula filter membrane filter 10 in blake bottle 1.
In this embodiment, the filter 5 has a pore size of 0.22 μm. The specific using method comprises the following steps:
1. filling the culture bottle 1 with sand-filtered seawater with the total volume of 1/3-1/2 of the culture bottle 1, screwing the bottle cap 2 and the ventilation adjusting switch 7, packaging the ventilation hose 8 separately, putting the packaged ventilation hose into an autoclave, sterilizing the packaged ventilation hose at 121 ℃ for 20 min, taking the packaged ventilation hose out of the autoclave, and cooling the packaged ventilation hose to room temperature;
2. the experimental culture device is suitable for culturing the prawns in different culture stages, but the stocking density of the prawns in different stages is different, the young prawns in the growth period is less than 1 month, the stocking density is 1 shrimp/10-20 ml of seawater, the growing period is more than 1 month of the prawns, the stocking density is more than 50 ml of seawater for each shrimp, and when the prawns are large, the device can also culture a single shrimp;
3. harvesting the prawns from the culture pond, washing the prawns with sterile seawater for three times, sterilizing the prawns in a 0.2 wt% sodium hypochlorite solution for 20-60 s, washing the prawns with the sterile seawater for three times, and washing off microorganisms adsorbed on the surface of the prawns, wherein the step needs to be carried out on a sterile super clean bench;
4. placing the sterilized and cooled culture bottle 1 into a sterile super clean bench, opening the bottle cap 2, placing the sterilized prawn, screwing the bottle cap 2, connecting the ventilation hose 8 and the sterile syringe type filter membrane filter 10, moving the sterile syringe type filter membrane filter into a culture room, opening the ventilation pump 9 to supply air, and adjusting the ventilation adjusting switch 7 to enable the air flow to be proper.
The above embodiments are further illustrative of the present invention, but the scope of the present invention is subject to the claims.
Claims (2)
1. The utility model provides a sterile breeding device of shrimp for laboratory which characterized in that: including the blake bottle that is used for breeding the shrimp, the upper end of blake bottle be provided with the bottle lid, the bottle lid on be provided with air vent and intake pipe, air vent department cover and be used for preventing that the microorganism gets into the blake bottle in the filter membrane, the one end of intake pipe stretch into the blake bottle in lower part and its other end be connected with the glass return bend, the mouth of pipe department of glass return bend be provided with the regulating switch that ventilates, the regulating switch that ventilates pass through the breather hose and connect the breather pump, the breather hose on set up and be used for preventing that the microorganism gets into the blake bottle in the syringe type filter membrane filter.
2. The sterile prawn cultivation device for the laboratory according to claim 1, which is characterized in that: the pore diameter of the filter membrane is 0.22 μm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202023034540.1U CN215012696U (en) | 2020-12-16 | 2020-12-16 | A small-size aseptic breeding device of shrimp for laboratory |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202023034540.1U CN215012696U (en) | 2020-12-16 | 2020-12-16 | A small-size aseptic breeding device of shrimp for laboratory |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN215012696U true CN215012696U (en) | 2021-12-07 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202023034540.1U Active CN215012696U (en) | 2020-12-16 | 2020-12-16 | A small-size aseptic breeding device of shrimp for laboratory |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN215012696U (en) |
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2020
- 2020-12-16 CN CN202023034540.1U patent/CN215012696U/en active Active
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