CN110423681A - A kind of photosynthetic microorganism culture production system - Google Patents

A kind of photosynthetic microorganism culture production system Download PDF

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CN110423681A
CN110423681A CN201910874536.9A CN201910874536A CN110423681A CN 110423681 A CN110423681 A CN 110423681A CN 201910874536 A CN201910874536 A CN 201910874536A CN 110423681 A CN110423681 A CN 110423681A
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culture
pipeline
unit
air
water
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肖卫武
程俊
罗彬�
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Abstract

The invention discloses a kind of photosynthetic microorganism culture production systems, including aeration cycle system, the aeration cycle system includes air supply unit, vent line unit, ball, solenoid valve and valve composition, the air supply unit includes air extractor, supply end cleaning equipment and air pressure precision detection controller, electromagnetic valve controlling system, the air pressure precision detection controller is mounted on the both ends of culture pipeline, the air extractor is accurately supplied using active air filters pressing cleaning equipment, the air extractor is for keeping culture pipeline internal and external pressure difference to be no more than ± 50Pa, the air extractor and gas supply end cleaning equipment are mounted on the both ends of culture pipeline, the present invention has filled up the defect of both current representative culture device systems, and it will cultivate, filtering, cleaning, sterilizing combines together, the safety for greatly strengthening equipment makes With the service life, workload is significantly reduced, significantly improves training efficiency, and significantly improve product quality.

Description

A kind of photosynthetic microorganism culture production system
Technical field
The present invention relates to field of microbial culture technology, specially a kind of photosynthetic microorganism culture production system.
Background technique
It is always the pursuit of people using the microorganism that sunlight large-scale culture is beneficial to man, successively emerges in large numbers both at home and abroad Numerous related invention patents are gone out.Favored with pipeline culture device patent compared with by people in these patents of invention --- training More efficient, product quality is more excellent.Pipeline culture device is broadly divided into two classes: one kind is green that precious health care product is limited with Shenzhen Company and Haikou Ka Siha are apt to the negative pressure of vacuum running equipment (culture systems) that biological photosynthetic reactor Co., Ltd is representative, One kind is the above sea light language and Leqing Guang Yu Biotechnology Co., Ltd is the positive pressure driving gas supply production system of representative.The former Middle equipment (pipeline) and microorganism are chronically at vacuum low pressure state, easily cause pipeline breaking (air pressure inwardly acts on tube wall) Grown poverty-stricken (hypocapnia) with microorganism, and air cannot effectively purify (if the aperture of filter plant is meticulous, will Culture solution is caused to be extracted outside pipe);And the latter is although it is noted that the former defect, but equipment is to be chronically at positive pressure shape State equally easily causes pipeline breaking (gas is opened outward, is acting outwardly against tube wall) and microorganism growth inhibition phenomenon --- because The algae that we select all is normal pressure or turns out close in the state of normal pressure to come.And either positive pressure expulsion operation is gone back It is that negative pressure pushes operation, is all the movement for driving liquid in pipeline by the movement of bubble (group), since gas density is small, always On the characteristic bubbled through the water column cause nearly all to be that supernatant liquid is flowing, and lower layer microorganism (algae) and liquid are several in pipeline It is motionless, it is easy to cause lower layer microorganism (algae) aggregation dead spoiled with liquid in this way --- internal diameter of the pipeline is bigger, this existing As being more obvious, for this purpose, it is proposed that a kind of photosynthetic microorganism culture production system.
Summary of the invention
The purpose of the present invention is to provide a kind of photosynthetic microorganism culture production system, the present invention has not only filled up current The defect of both representative culture device systems, and culture, filtering, cleaning, sterilizing are combined together, it greatly strengthens and sets Standby safe operating life, significantly reduces workload, significantly improves training efficiency, and significantly improves product product Matter.
To achieve the above object, the invention provides the following technical scheme: a kind of photosynthetic microorganism culture production system, including
Aeration cycle system, the aeration cycle system include air supply unit, vent line unit, ball, solenoid valve and valve Door composition, the air supply unit include air extractor, gas supply end cleaning equipment and air pressure precision detection controller, the air pressure essence Secret agent surveys the both ends that controller is mounted on culture pipeline, and the air extractor is accurately supplied using active air filters pressing cleaning equipment Gas, for the air extractor for keeping culture pipeline internal and external pressure difference to be no more than ± 50Pa, the air extractor and gas supply end are net Change the both ends that equipment is mounted on culture pipeline;
Water system, the water system include water supplying unit, water-filled pipe unit, flow controlling unit and valve unit group At the water-filled pipe unit includes transfer pipeline, and the flow controlling unit includes flowmeter, and the valve unit is by multiple groups Conversion connector is arranged at turning in Draw off valve composition, the culture pipeline, and converts and be equipped with Draw off valve on connector, The Draw off valve is connected to water-filled pipe, and the tail end of water-filled pipe is connected on water supply device, and the flowmeter is mounted on On water-filled pipe;
Cleaning system, the cleaning system include tube wall rubbish detection unit, cleaning unit and filter element, the cleaning unit For moveable ball, the ball setting is in culture pipeline, and the diameter of ball and culture pipeline inner diameter Identical, the surface of the ball is contacted with culture inner wall of the pipe, and the tube wall rubbish detection unit uses tube wall detector, and Tube wall detector is mounted on culture pipeline, and the filter element is liquid separating filter;
Feeding system, the feeding system include feed unit, feed time judging unit and growth encourage unit, the feed unit For providing culture solution in pipeline to cultivate, and feed unit is connect with culture pipeline, and the feed time judging unit is used for Timing is fed, and feed time judging unit and feed unit are electrically connected, and the growth encourage unit includes ozone generator And carbon dioxide air source, the ozone generator are used to carry out ozone sterilization to culture solution, the carbon dioxide air source is for dilute Release the gas concentration lwevel driven in the residue ozone in culture solution and increase culture solution;
Purification filtering system, including air filtration, culture solution filtering and impurity separating and filtering, air filtration are net using air Change device, water filtration is filtered using sand stick, and impurity separating and filtering uses separatory funnel;
It further include control unit and movable purifying vacuum decompression filtering collecting cart, described control unit and feeding system are cleared up System, water system are connected with aeration cycle system, and the movable purifying vacuum decompression filtering collecting cart is used for each training Support the harvesting of pipeline microorganism.
Preferably, one end of the culture pipeline is equipped with suction port, and the other end of culture pipeline is equipped with inlet end Mouthful, and suction port and air inlet port are arranged at the straight tube road of culture pipeline, the head and the tail of the culture pipeline are interconnected Closed loop is formed, realizes the variation of draught head.
Preferably, the air extractor is connected to the suction port of culture pipeline, the gas supply end cleaning equipment and culture The air inlet port of pipeline is connected to, and is easy to implement the transformation of pressure directions.
Preferably, water tank can be used in the water supply device, is convenient for supplying water.
Preferably, liquid separating filter includes filter tunnel and electromagnetic valve, and the filter tunnel is mounted on culture pipeline At air inlet port, the electromagnetic valve is mounted on the water outlet of filter tunnel, divides convenient for impurity and the automatic of other sediments From.
Preferably, the ozone generator and carbon dioxide air source are mounted on gas supply end cleaning equipment, and ozone occurs It is connected between device and carbon dioxide air source by gas pipeline, is sterilized using ozone generator to culture solution before culture and then existed Carbon dioxide air source is supplemented in incubation and helps microorganism growth, while diluting or driving residual ozone.
Preferably, start ozone generator after addition culture solution and before inoculation every time, to culture solution ozone sterilization Exhaust, and sterilization time is 30 minutes, is then vented and will import carbon dioxide air source in culture solution, to drive most training as early as possible Residue ozone in nutrient solution is that an appropriate environment is created in the culture growth of microorganism.
Preferably, the movement of the ball, by the draught head direction of transformation culture pipe ends, it can be achieved that ball Round-trip movement, ball move achievable liquid and integrally circulate, and ball and tube wall are rubbed back and forth so as to manage Wall cleans up.
Preferably, the culture pipeline is changed to respectively tilt two long rectangle faces by traditional rectangular duct plane vertical arrangement 45-60 ° of increase lighting efficiency.
The present invention provides a kind of photosynthetic microorganism culture production system, have it is following the utility model has the advantages that
(1) present invention is by disposing air pressure precision detection controller in culture pipe ends, and uses active empty in air inlet port Cleaning equipment is filtered in air pressure, and in order to provide accurate gas supply, while suction port is evacuated using air extractor, to keep cultivating Pipeline internal and external pressure difference is no more than ± 50Pa.
(2) present invention is passed through by placing a ball identical with culture pipeline diameter in culture pipeline The gas pressure intensity difference of pipe ends is cultivated to push ball to run in culture pipeline, and then pushes culture solution and microorganism whole Body circulates, and the process that ball is run in culture pipeline is understood while will be attached to the impurity or microorganism band of tube wall Get off.
(3) present invention is passed through by placing a ball more bigger with culture pipe diameter in culture pipeline The gas pressure intensity difference of pipe ends is cultivated to push ball to run in culture pipeline, transformation pressure difference direction is to push rubber Glueballs moves back and forth in pipeline, so that the attached crop friction of tube wall be washed down.So as to avoid traditional disassembly, cleaning Mode realizes the automated cleaning of pipeline, improves cleaning efficiency.
(4) present invention is added every time by disposing ozone generator and carbon dioxide air source after supplying end cleaning equipment After culture solution, start ozone generator before inoculation, ozone sterilization is carried out to culture solution, then imports titanium dioxide to culture solution Carbon further dilutes the residue ozone driven in culture solution, while increasing the gas concentration lwevel in culture solution, is conducive to photosynthetic The growth of microorganism.
(5) present invention is by installing liquid separating filter in the air inlet port lower end of straight tube, using gravity by impurity and Other sediments are automatically separated.
(6) rectangular duct is changed to two by the present invention by changing traditional rectangular duct plane homeotropic texture Long rectangle face respectively tilts 45-60 ° of structure, allows the pipeline of mounted on top sufficiently to increase lighting efficiency, while enhancing and being The stability of system.
(7) present invention by outfit movable purifying be filtered under diminished pressure collecting cart, it can be achieved that each pipeline microorganism it is convenient Harvesting, significantly improves working efficiency.
The present invention has filled up the defect of both current representative culture device systems, and will cultivate, and filter, is clear It washes, sterilize and combine together, greatly strengthen the safe operating life of equipment, significantly reduce workload, significantly improve culture Working efficiency, and significantly improve product quality.
Detailed description of the invention
Fig. 1 is system structure diagram of the invention;
Fig. 2 is culture pipeline structural schematic diagram of the invention;
Fig. 3 is ball structural schematic diagram of the invention;
Fig. 4 is liquid separating filter structural schematic diagram of the invention.
In figure: 1, air extractor;2, suction port;3, Draw off valve;4, connector is converted;5, pipeline is cultivated;6, ozone Generator;7, carbon dioxide air source;8, gas pipeline;9, end cleaning equipment is supplied;10, liquid separating filter;11, air inlet port; 12, filter tunnel;13, electromagnetic valve;14, ball.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete Site preparation description.
As shown in Figs 1-4, the present invention provides a kind of technical solution: a kind of photosynthetic microorganism culture production system, including
Aeration cycle system, the aeration cycle system include air supply unit, vent line unit, ball 14 and valve group At the air supply unit includes air extractor 1, gas supply end cleaning equipment 9 and air pressure precision detection controller, and the air pressure is accurate Detection controller is mounted on the both ends of culture pipeline 5, and the air extractor 1 is accurately supplied using active air filters pressing cleaning equipment Gas, the air extractor 1 is for keeping culture 5 internal and external pressure difference of pipeline to be no more than ± 50Pa, the air extractor 1 and gas supply end Cleaning equipment 9 is mounted on the both ends of culture pipeline 5;
Water system, the water system include water supplying unit, water-filled pipe unit, flow controlling unit and valve unit group At the water-filled pipe unit includes transfer pipeline, and the flow controlling unit includes flowmeter, and the valve unit is by multiple groups Draw off valve 3 forms, and conversion connector 4 is arranged at turning in the culture pipeline 5, and converts to be equipped on connector 4 and discharge water Valve 3, the Draw off valve 3 is connected to water-filled pipe, and the tail end of water-filled pipe is connected on water supply device, the flowmeter It is mounted on water-filled pipe;
Cleaning system, the cleaning system include tube wall rubbish detection unit, cleaning unit and filter element, the cleaning unit For moveable ball 14, the setting of ball 14 is in culture pipeline 5, and the diameter of ball 14 and culture pipeline 5 Inner diameter is identical, and the surface of the ball 14 is contacted with culture 5 inner wall of pipeline, and the tube wall rubbish detection unit is using pipe Wall detector, and tube wall detector is mounted on culture pipeline 5, the filter element is liquid separating filter 10;
Feeding system, the feeding system include feed unit, feed time judging unit and growth encourage unit, the feed unit For providing culture solution in pipeline 5 to cultivate, and feed unit is connect with culture pipeline 5, and the feed time judging unit is used In being periodically fed, and feed time judging unit and feed unit are electrically connected, and the growth encourage unit includes ozone Device 6 and carbon dioxide air source 7, the ozone generator 6 are used to carry out ozone sterilization, the carbon dioxide air source 7 to culture solution For diluting the gas concentration lwevel driven in the residue ozone in culture solution and increase culture solution;
It further include control unit and movable purifying vacuum decompression filtering collecting cart, described control unit and feeding system are cleared up System, water system are connected with aeration cycle system, and the movable purifying vacuum decompression filtering collecting cart is used for each training Support the harvesting of 5 microorganism of pipeline.
Preferably, one end of the culture pipeline 5 is equipped with suction port 2, and the other end of culture pipeline 5 is equipped with air inlet Port 11, and suction port 2 and air inlet port 11 are arranged at the straight tube road of culture pipeline 5, the head and the tail of the culture pipeline 5 It is interconnected and forms closed loop, the variation of air inlet port 11 and the realization draught head of suction port 2.
Preferably, the air extractor 1 with culture pipeline 5 suction port 2 be connected to, gas supply end cleaning equipment 9 and The air inlet port 11 for cultivating pipeline 5 is connected to, and air extractor 1 and gas supply end cleaning equipment 9 are mounted on to the both ends of culture pipeline 5, It is easy to implement the transformation of pressure directions.
Preferably, water tank can be used in the water supply device, is supplied water using water tank.
Preferably, liquid separating filter 10 includes filter tunnel 12 and electromagnetic valve 13, and the filter tunnel 12 is mounted on training At the air inlet port 11 for supporting pipeline 5, the electromagnetic valve 13 is mounted on the water outlet of filter tunnel 12, utilizes liquid separating filter 10 are automatically separated impurity and other sediments.
Preferably, the ozone generator 6 and carbon dioxide air source 7 are mounted on gas supply end cleaning equipment 9, and ozone is sent out It is connected between raw device 6 and carbon dioxide air source 7 by gas pipeline 8, is helped using ozone generator 6 and carbon dioxide air source 7 Microorganism growth.
Preferably, start ozone generator 6 after addition culture solution and before inoculation every time, to culture solution ozone sterilization Exhaust, and sterilization time is 30 minutes, is then vented, while carbon dioxide air source 7 being imported into culture solution, utilizes titanium dioxide Carbon further dilutes the residue ozone driven in culture solution, while increasing the gas concentration lwevel in culture solution, is conducive to photosynthetic The growth of microorganism.
Preferably, draught head direction of the back and forth movement of the ball 14 by transformation culture 5 both ends of pipeline, ball 14 carry out back and forth movement under the variation of pressure directions, so as to push culture pipeline 5 in liquid flowing, ball 14 it is past Movement is returned by the draught head direction at transformation culture 5 both ends of pipeline, ball 14 is transported back and forth under the variation of pressure directions It is dynamic, the flowing of the liquid in culture pipeline 5 can be pushed, and realize pipeline automatically cleaning.
Preferably, the culture pipeline 5 is changed to respectively tilt two long rectangle faces by traditional rectangular duct plane vertical arrangement 45-60 °, so that the culture pipeline 5 of mounted on top can be contacted with sunray, increase illuminating area, improves daylighting effect Rate is more conducive to microorganism growth.
Preferably, the entirety of culture solution, which circulates, is realized by the movement of ball 14.
It should be noted that a kind of photosynthetic microorganism culture production system, at work, due to cultivating the pumping of pipeline 5 Port 2 and air inlet port 11 are respectively mounted air pressure precision detection controller, in use, the gas supply end cleaning equipment 9 of air inlet port 11 It is supplied for it, when the air pressure in culture pipeline 5 is more than ± 50Pa with ambient pressure difference, starts the pumping of suction port 2 Device 1 carries out pumping activity, so that the draught head inside and outside culture pipeline 5, which is no more than ± 50Pa, passes through confession when needing to clean Gas end cleaning equipment 9 and air extractor 1 convert the draught head direction at culture 5 both ends of pipeline, so that ball 14 is being trained Back and forth movement in pipeline 5 is supported, the wherein movement in culture pipeline 5 of ball 14 will push following for entire microbial body and culture solution Circulation is dynamic, simultaneously as 14 diameter of ball is identical as culture 5 internal diameter of pipeline, the mistake that ball 14 is run in culture pipeline 5 Journey can wipe the impurity for being attached to tube wall or microorganism, to achieve the effect that clear up tube wall, in addition culture solution every time Later and before inoculation, start ozone generator 6, is sterilized 30 minutes using the ozone that ozone generator 6 generates to culture solution, so After carry out gas exhaust treatment, then the carbon dioxide in carbon dioxide air source 7 is imported in culture solution and further dilutes and drive culture Residue ozone in liquid, while increasing the gas concentration lwevel in culture solution, which is conducive to the growth of photosynthetic microorganism, Since the effect of gravity will appear sinkage, the impurity or sediment of sinking pass through separatory filtration process for middle impurity and other sediments Device 10 can be separated, and the present invention changes traditional rectangular duct plane homeotropic texture, by rectangular duct be changed to by Two long rectangle faces respectively tilt 45-60 ° of structure, and the pipeline of mounted on top is sufficiently contacted with illumination, increase lighting efficiency, The stability of system is enhanced simultaneously, after cultivating successfully, directly collecting cart can be filtered under diminished pressure using movable purifying and be harvested.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with A variety of variations, modification, replacement can be carried out to these embodiments without departing from the principles and spirit of the present invention by understanding And modification, the scope of the present invention is defined by the appended.

Claims (9)

1. a kind of photosynthetic microorganism culture production system, which is characterized in that including
Aeration cycle system, the aeration cycle system include air supply unit, vent line unit, ball (14), solenoid valve And valve composition, the air supply unit include air extractor (1), supply end cleaning equipment (9) and air pressure precision detection controller, The air pressure precision detection controller is mounted on the both ends of culture pipeline (5), and the air extractor (1) uses active air filters pressing Cleaning equipment accurately supplies, and the air extractor (1) is for keeping culture pipeline (5) internal and external pressure difference to be no more than ± 50Pa, institute It states air extractor (1) and supplies the both ends that end cleaning equipment (9) are mounted on culture pipeline (5);
Water system, the water system include water supplying unit, water-filled pipe unit, flow controlling unit and valve unit group At the water-filled pipe unit includes transfer pipeline, and the flow controlling unit includes flowmeter, and the valve unit is by multiple groups Draw off valve (3) composition, conversion connector (4) is arranged at turning in the culture pipeline (5), and converts and pacify on connector (4) Equipped with Draw off valve (3), the Draw off valve (3) is connected to water-filled pipe, and the tail end of water-filled pipe is connected to water supply device On, the flowmeter is mounted on water-filled pipe;
Cleaning system, the cleaning system include tube wall rubbish detection unit, cleaning unit and filter element, the cleaning unit For moveable ball (14), ball (14) setting is in culture pipeline (5), and the diameter of ball (14) and training Feeding pipeline (5) inner diameter is identical, and the surface of the ball (14) is contacted with culture pipeline (5) inner wall, the tube wall rubbish Detection unit uses tube wall detector, and tube wall detector is mounted in culture pipeline (5), and the filter element is separatory filtration process Device (10);
Feeding system, the feeding system include feed unit, feed time judging unit and growth encourage unit, the feed unit For providing culture solution for culture pipeline (5) is interior, and feed unit is connect with culture pipeline (5), and the feed time judgement is single Member is fed for timing, and feed time judging unit and feed unit are electrically connected, and the growth encourage unit includes ozone Generator (6) and carbon dioxide air source (7), the ozone generator (6) are used to carry out ozone sterilization, the dioxy to culture solution Change carbon gas source (7) and is used to dilute the gas concentration lwevel driven in the residue ozone in culture solution and increase culture solution;
It further include control unit and movable purifying vacuum decompression filtering collecting cart, described control unit and feeding system are cleared up System, water system are connected with aeration cycle system, and the movable purifying vacuum decompression filtering collecting cart is used for each training Support the harvesting of pipeline (5) microorganism.
2. a kind of photosynthetic microorganism culture production system according to claim 1, it is characterised in that: the culture pipeline (5) one end is equipped with suction port (2), and the other end of culture pipeline (5) is equipped with air inlet port (11), and suction port (2) and air inlet port (11) is arranged at the straight tube road for cultivating pipeline (5), and the head and the tail of culture pipeline (5) are interconnected Form closed loop.
3. a kind of photosynthetic microorganism culture production system according to claim 1, it is characterised in that: the air extractor (1) it is connected to the suction port (2) of culture pipeline (5), gas supply end cleaning equipment (9) and the inlet end for cultivating pipeline (5) Mouth (11) connection.
4. a kind of photosynthetic microorganism culture production system according to claim 1, it is characterised in that: the water supply device can Using water tank.
5. a kind of photosynthetic microorganism culture production system according to claim 1, it is characterised in that: the liquid separating filter It (10) include filter tunnel (12) and electromagnetic valve (13), the filter tunnel (12) is mounted on the inlet end of culture pipeline (5) At mouth (11), the electromagnetic valve (13) is mounted on the water outlet of filter tunnel (12).
6. a kind of photosynthetic microorganism culture production system according to claim 1, it is characterised in that: the ozone generator (6) it is mounted on gas supply end cleaning equipment (9) with carbon dioxide air source (7), and ozone generator (6) and carbon dioxide air source (7) it is connected between by gas pipeline (8).
7. a kind of photosynthetic microorganism culture production system according to claim 1, it is characterised in that: add culture solution every time Start ozone generator (6) later and before inoculation, culture solution ozone sterilization is vented, and sterilization time is 30 minutes, so Final vacuum, while carbon dioxide air source (7) being imported into culture solution.
8. a kind of photosynthetic microorganism culture production system according to claim 1, it is characterised in that: the ball (14) Back and forth movement by transformation culture pipeline (5) both ends draught head direction.
9. a kind of photosynthetic microorganism culture production system according to claim 1, it is characterised in that: the culture pipeline (5) it is changed to two long rectangle faces respectively tilting 45-60 ° by traditional rectangular duct plane vertical arrangement.
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