CN102000679A - Method for cleaning inner wall of pipeline type photobioreactor pipe - Google Patents
Method for cleaning inner wall of pipeline type photobioreactor pipe Download PDFInfo
- Publication number
- CN102000679A CN102000679A CN 201010500926 CN201010500926A CN102000679A CN 102000679 A CN102000679 A CN 102000679A CN 201010500926 CN201010500926 CN 201010500926 CN 201010500926 A CN201010500926 A CN 201010500926A CN 102000679 A CN102000679 A CN 102000679A
- Authority
- CN
- China
- Prior art keywords
- pipeline
- bioreactor
- magnetic spheres
- algae
- magnetic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention belongs to the biologic field of micro algae, relating to a method for cleaning inner wall of pipeline type photobioreactor pipe in micro algae culture. Magnetic sphere is placed into a pipeline type photobioreactor, culture solution flows when being pushed by power, the magnetic sphere randomly enters into the pipeline of photobioreactor along with the culture solution and does directional movement or slides under manual regulation of a control system outside the pipeline, and the magnetic sphere continuously slightly collides and rubs pipe wall in circulated moving process in the photobioreactor pipeline, so that algae cell material adhered onto transparent pipeline inner wall is rubbed into liquid phase used for culturing micro algae, the pipeline photobioreactor pipeline is effectively cleaned and light transmission thereof is maintained. The invention can effectively clean cells into algae liquid phase at initial stage of adherence, or substance such as cell adhered to the inner wall of the pipeline type photobioreactor pipe is removed, restriction of micro algae cell adherence to micro algae cell culture is overcome, and good light transmission and culture effect of photobioreactor are maintained.
Description
Technical field
The invention belongs to little algae biological field, relate to a kind of method for cleaning of pipeline bioreactor inside pipe wall of little algae cultivation.
Background technology
Little algae is unique bait of aquatic livestock whole growth process in the aquatic ecosystem (or specific stage of development) or the bait of food organisms as the important component part of primary productive force in the biosphere.Simultaneously, little algae is near floristic 50%, has the diversity of going out, complexity and particularity at aspects such as germplasm, ecological distribution, hereditary information, biochemical composition, metabolic pathways, further determined nutrition and pharmacy value that it is potential, the mankind may therefrom develop the special high added value bioactive ingredients of a large amount of structures.
The energy be pillar of economy also be the motive force of social development, determining modern civilization progress degree.Be accompanied by global economy rapid growth and urbanization process and accelerate, in the world the petroleum-based energy demand is increased day by day, traditional energies such as while oil all have non-renewable, exhausted because of a large amount of exploitations at last, so the petroleum-based energy shortage problem becomes increasingly conspicuous.In recent years, be the new force of regenerative resource in the world as the biodiesel of one of substitute of fossil energy, development is swift and violent, range of application constantly enlarges, and proportion grows steadily in diesel oil market.Wherein little algae has photosynthetic efficiency height, oil content height, growth cycle weak point, grease productivity per unit height, help reducing the gentle unique advantage such as environment pressure of separating of carbon emission with the absorbing carbon dioxide coupling, therefore develop little algae bioenergy (biodiesel etc.) be considered to development potentiality huge, most possibly substitute the bioenergy of oil.
Yet there is not a large amount of ready-made little algae resource that can directly collect processing in nature.Exploitation is at first wanted the scale engineering to cultivate and is produced little algae raw material for the required little algae resource of human social development.In the world, micro-algae large-scale is manually cultivated and is mainly contained open pond and closed photobioreactor 2 class modes before little Cutleriales:
Open cultivation (as circulation runway pond) is simple relatively, investment is low.But this mode condition of culture changes greatly, and little algae productive rate is low, and has serious biological pollution.Any natural enemy biology (as protozoan) and other algae that comes from water source, air, fertilizer, instrument and operation etc. all very easily invaded pollution, usually causes whole cultivation utter failure, directly limited the little algae of influence and cultivated and development of resources.Have only little algae that only a few can grow in extreme environment (as in high pH environment fast the spirulina of growth and in high salinity, become the salt algaes of sociales) can utilize this mode successfully to cultivate.
Though the closed photo bioreactor investment cost is relatively large, owing to can regulate and control multinomial culture parameters, be easier to control biological pollution, the productive rate of cultivating little algae is higher, therefore becomes the important trend of this area research and development in recent years.The closed photo bioreactor that has been used for laboratory and lab scale research (working volume from several be raised to several hectolitres) at present is a lot, and pot type bioreactor, dull and stereotyped bioreactor, column bioreactor, pipeline (vertical, parallel, spiral) bioreactor are arranged.But, amplify to cultivate several ton to up to a hundred ton the closed photo bioreactor of sizable application in produce veritably but seldom.Cause the related reason of this situation a lot, (its resistance to compression and high temperature resistant property are very low not only to comprise the physicochemical property of translucent material (glass, lucite and plastics), be easy to fragmentation and with thermal deformation even fusing etc.) restriction, and need effectively compensate carbon dioxide-depleted, the too high dissolved oxygen of efficient parsing is avoided high oxygen injury, and keeps the reactor body surface area and do not descend degradation when volume of culture increases.At present, the development of pipeline bioreactor can address the above problem to a certain extent, becomes one of major technique means that increase the bioreactor volume gradually.
It may be noted that bioreactor (particularly pipeline bioreactor) is inner very easily takes place and the cell attachment phenomenon occurs adhering to of microalgae cell etc.How effectively to remove these attachments, ensureing the light transmission of bioreactor and keeping high culture efficiency is the problem that bothers very much, this is not had good solution in the past.Along with little algae biotechnology and little algae industry development, cleaning and remove adherent little algae has become little algae biological technical field research focus at home and abroad, is badly in need of solving in development and application large-scale pipeline bioreactor.
Little Trentepohlia has characteristics such as phototaxis, photophobism, adherent growth concurrently in photoautotroph, and in little algae incubation, cell usually is easy to paste reactor tube walls etc. and locates.The adherent degree of microalgae cell is not only relevant with different microalgae self biological characteristics, and is also relevant with algae liquid flow rate.
In pipeline, pipeline zone line flow velocity is the fastest, and slow more near the near more flow velocity of tube wall, is close to zero at the regional flow velocity near tube wall, and this zone is the dead angle of bioreactor.In any bioreactor, all there is above-mentioned dead angle area (dead angle also comprises the positions such as turning in the reactor) inevitably.Generally speaking, algae liquid overall flow speed is fast more, and is just few more near the dead angle area of low flow velocity of tube wall even zero flow velocity; Otherwise algae liquid flow rate is slower, and low flow velocity of close tube wall and zero flow velocity dead angle area are the more.
At dead angle area, algae liquid is mixing fully, and sedimentation or adherent growth easily take place.In the pipeline bioreactor, regardless of algae liquid overall flow speed, always exist, so adherent situation takes place just unavoidably in the cultured microalgae cell at low flow velocity of close tube wall even zero flow velocity zone.
In a single day microalgae cell takes place adherent, produces vicious circle and forms following serial adverse effect, and the cell culture effect is constantly descended, even causes whole cultivating system collapse.At first, microalgae cell takes place adherent behind reactor wall, cause the reactor material rough surface, increased fluid resistance, reduced the algae flow velocity, further increased dead angle quantity in the bioreactor, made the adherent phenomenon of the easier generation of cell, the increasing vicious circle of cell attachment degree; Secondly, after microalgae cell is adherent, formed little algae spot (or little algae thin layer) also is every luminous point (or even every photosphere), influence the translucidus of bioreactor to some extent, hinder light and entered bioreactor inside, so insufficient light that inner microalgae cell absorbs, the microalgae cell photosynthetic efficiency descends; The 3rd, be in the high light state all the time near the attached cell of pipeline, light supersaturation and light injury takes place causes this part cell photosynthetic efficiency to descend, even cell death takes place.In addition, if after the attached cell death, frond takes place to rot, and also influences little algae product quality.
Summary of the invention
In large-scale closed conduit bioreactor application process, the adherent problem of microalgae cell unavoidably appears, takes place for reducing the adherent phenomenon of little algae, keep bioreactor light transmission and culture effect.The object of the present invention is to provide a kind of method for cleaning of pipeline bioreactor inside pipe wall of little algae cultivation, can just it effectively be cleaned in the algae liquid phase at the cell attachment early period of origination effectively, the materials such as attached cell that maybe will stick on the pipeline bioreactor inside pipe wall are removed, overcome the adherent restriction that microalgae cell is cultivated of microalgae cell, keep bioreactor good light transmittance and culture effect.
The present invention solves and to be adsorbed on the scheme that the adsorbates such as adherent microalgae cell on the pipeline bioreactor inside pipe wall take and to be:
A kind of method for cleaning of pipeline bioreactor inside pipe wall, between the inlet ductwork and export pipeline of pipeline bioreactor, the circulating line bypass is set, in the circulating line bypass, gauge tap is set, in the circulatory system of circulating line bypass and pipeline bioreactor formation, sets up at least one magnetic spheres;
Magnetic spheres is put into the pipeline bioreactor, nutrient solution begins to flow under dynamic action, magnetic spheres enters in the pipeline of bioreactor at random and carries out orientation and move with nutrient solution, perhaps be subjected to pipeline external control system system's manual adjustment and slide, magnetic spheres constantly produces slight impact and friction with tube wall in the circulation moving process in the Photoreactor pipeline, thereby the frustule material that will paste on the transparent pipeline inwall rubs in the liquid phase of the little algae of cultivation, the pipeline bioreactor is effectively cleared up and is kept its light transmission.
The method for cleaning of described pipeline bioreactor inside pipe wall, the magnetic spheres occluding device is set on the export pipeline of pipeline bioreactor, and the magnetic spheres occluding device is positioned at the circulatory system outside of circulating line bypass and pipeline bioreactor formation;
Magnetic spheres arrives the occluding device position after finishing the cleaning of pipeline bioreactor, and magnetic spheres is blocked, and nutrient solution enters the recovery of algae liquid, gas exchange and temperature control tower smoothly by this magnetic spheres occluding device; The magnetic spheres of being obstructed enters the circulating line bypass under the effect of gravity and magnetic attracting force, reclaim, be deposited in the upper side position of gauge tap.
The method for cleaning of described pipeline bioreactor inside pipe wall, the magnetic spheres occluding device adopts the network structure that can stop magnetic spheres to be passed through.
The method for cleaning of described pipeline bioreactor inside pipe wall, the size of each magnetic spheres equates with the pipeline internal diameter of bioreactor substantially, the pipeline of bioreactor and magnetic spheres adopt matched in clearance, and magnetic spheres is just moved at the pipeline of bioreactor.
The method for cleaning of described pipeline bioreactor inside pipe wall, magnetic spheres has the inside and outside double-layer structure that is provided with, inner can produce magnetic or by inducing the material of generation magnetic for rigidity, outside plastic material for wrapping up magnet, avoiding its crash response organ pipe wall, have certain shrinkage, the overall proportion of magnetic spheres equates with the proportion of micro algae culturing liquid or is bigger.
The method for cleaning of described pipeline bioreactor inside pipe wall, the mesh aperture on the occluding device be less than whole magnetic spheres diameter, and the magnet diameter middle than magnetic spheres of the mesh aperture on the occluding device is little.
The method for cleaning of described pipeline bioreactor inside pipe wall attracts control device in pipeline bioreactor peripheral hardware magnetic spheres, and magnetic spheres attracts control device guiding magnetic spheres to enter the circulation bypass or rest on the somewhere.
The method for cleaning of described pipeline bioreactor inside pipe wall, one end of circulating line bypass be connected pipeline bioreactor light accepting part divide with algae liquid reclaim, on the pipeline between gas exchange and the temperature control tower, magnetic spheres attraction control device is arranged at an end of described circulating line bypass; The other end of circulating line bypass is connected between pipeline bioreactor light accepting part branch and the kinetic pump, and the switching of this circulating line bypass is controlled by the ball-type gauge tap.
The method for cleaning of described pipeline bioreactor inside pipe wall, kinetic pump, switch I, switch I I are set on the inlet ductwork of pipeline bioreactor, switch I, kinetic pump are positioned at a side of circulating line bypass in order, and switch I I is positioned at the opposite side of circulating line bypass;
Close kinetic pump, open gauge tap, magnetic spheres is fallen the downside of gauge tap; Closing control switch then, restart kinetic pump, magnetic spheres reenters in the pipeline bioreactor, produce friction with tube wall, the frustule material that pastes on the transparent pipeline inwall is rubbed in the liquid phase of the little algae of cultivation, circulation is so repeatedly effectively cleared up the attached cell on the pipeline bioreactor and is kept its light transmission.
The method for cleaning of described pipeline bioreactor inside pipe wall, little algae come from freshwater microalgae, become in water or the briny environment, comprise flat algae, chlorella, chrysophyceae, grid algae, haematococcus, purple ball algae, snow algae or diatom etc.
Advantage of the present invention is:
1, magnetic spheres of the present invention circulates in the pipeline bioreactor by current and moves, and the magnetic spheres size equates substantially that with internal diameter of the pipeline therefore magnetic spheres and inner-walls of duct produce slight impact and friction in moving process.Therefore, can effectively remove, thereby ensure good light permeability in the bioreactor operation process and high culture efficiency attached to adherent microalgae cell on the inside pipe wall and other material.
2, magnetic spheres of the present invention has plasticity, has avoided hard collision, cut equivalent damage to pipeline, therefore the pipeline bioreactor is played the certain protection effect.
3, the present invention is provided with magnetic spheres occluding device and circulation bypass and gauge tap, realization cultivation algae liquid separates with magnetic spheres, under the effect of kinetic pump etc., not only keep functions such as the normal algae liquid of cultivating system flows, gas exchange (oxygen is resolved and the carbon dioxide compensation), exchange heat, and magnetic spheres need not pass through kinetic pump in pipe blow-through inwall process, thereby avoided kinetic pump generation mechanical damage.
4, magnetic spheres of the present invention can recycle in the closed conduit bioreactor for a long time, has reduced the biological pollution that bioreactor takes place in opening procedure repeatedly.
5, magnetic spheres making of the present invention is simple, cheap, and operation is simple, production application is very economical, has reduced the pipeline bioreactor and has manually cleaned difficulty and the high problem of cost, therefore has very big production application value.
6, therefore the inventive method can keep light transmission and its cultivation high efficiency of guarantee of pipeline bioreactor for microalgae cell and other attachment of effective removal adherent growth in the closed conduit bioreactor provide possibility.Be of value to scale light autotrophy and cultivate microalgae cell, aquaculture bait, the little algae germplasm of high-purity are provided and produce little algae bioenergy (as biodiesel etc.) and the little algae product of high added value.
Description of drawings
Fig. 1 is pipeline photo-bioreactor system of the present invention and inside pipe wall cleaning signal pattern diagram.
Among the figure: 1, magnetic spheres; 1a, plastic material; 1b, magnet; 2, magnetic spheres attracts control device; 3, circulating line bypass; 31 gauge taps; 4, pipeline bioreactor; 5, magnetic spheres occluding device; 6, algae liquid recovery, gas exchange and temperature control tower; 7, sensor; 8, kinetic pump; 9, switch I; 10, switch I I.
The specific embodiment
The present invention's design is: in pipeline bioreactor inside cleaning plant is set, and at the pipeline peripheral controller, but setting manual control switch's circulating line bypass on pipe-line system.
As shown in Figure 1, the pipeline bioreactor system mainly comprises: the inner cleaning magnetic spheres 1 that is provided with of pipeline bioreactor, attract in the magnetic spheres of pipeline bioreactor peripheral hardware that control device 2, the circulating line bypass 3 newly-increased on the pipe-line system, pipeline bioreactor 4, the magnetic spheres occluding device 5 in pipeline optical-biological reaction organ pipe, algae liquid reclaim, gas exchange and temperature control tower 6, various kinds of sensors 7, kinetic pump 8, switch I 9, switch I I10 etc., concrete structure is as follows:
Algae liquid reclaims, gas exchanges and the algae liquid of temperature control tower 6 exports the algae liquid import of passing through pipeline connecting pipe bioreactor 4, algae liquid reclaims, the algae liquid outlet of the algae liquid import of gas exchange and temperature control tower 6 by pipeline connecting pipe bioreactor 4, reclaims at algae liquid, various kinds of sensors 7 is set in gas exchange and the temperature control tower 6.
Unlike the prior art be: between the inlet ductwork and export pipeline of pipeline bioreactor 4, the circulating line bypass is set, promptly between two pipelines that algae liquid reclaims, gas exchanges and temperature control tower 6 communicates with pipeline bioreactor 4, increase circulating line bypass 3, gauge tap 31 is set in circulating line bypass 3; In the circulatory system that circulating line bypass 3 and pipeline bioreactor 4 constitute, set up the magnetic spheres 1 of at least one cleaning usefulness.
Reclaim at algae liquid, on the pipeline (being inlet ductwork) between gas exchange and outlet of temperature control tower 6 algae liquid and the 4 algae liquid imports of pipeline bioreactor kinetic pump 8 is set, switch I 9, switch I I 10, switch I 9, kinetic pump 8 is positioned at a side (circulatory system outside that circulating line bypass and pipeline bioreactor constitute) of circulating line bypass 3 in order, switch I I 10 is arranged in the opposite side (being positioned at the circulatory system that circulating line bypass and pipeline bioreactor constitute) of circulating line bypass 3, attracts control device 2 in pipeline bioreactor 4 peripheral hardware magnetic spheres.On the pipeline (being export pipeline) between the recovery of algae liquid, gas exchange and temperature control tower 6 algae liquid imports and the outlet of pipeline bioreactor 4 algae liquid magnetic spheres occluding device 5 is set, magnetic spheres occluding device 5 is positioned at outside the circulatory system of circulating line bypass 3 and pipeline bioreactor 4 formations.
One end of this circulating line bypass 3 be connected pipeline bioreactor 4 light accepting parts divide with algae liquid reclaim, on the pipeline between gas exchange and the temperature control tower 6, magnetic spheres attraction control device 2 is arranged at an end of described circulating line bypass 3.The other end of circulating line bypass 3 is connected between pipeline bioreactor 4 light accepting parts branch and the kinetic pump 8, and the switching of this circulating line bypass 3 is controlled by the ball-type gauge tap.
Among the present invention, magnetic spheres attracts control device 2 to refer to that all can produce the magnetic line of force, can attract object each other, as: magnet or electromagnetic coil.
Among the present invention, magnetic spheres occluding device 5 is adopted as network structure, has a lot of mesh on the device, and this occluding device does not allow magnetic spheres to pass through, but allows algae liquid to flow through.Mesh aperture on the occluding device, its size be not only less than whole magnetic spheres diameter, and also littler than the magnet diameter in the middle of the magnetic spheres 1.
As shown in Figure 1, the detailed process of pipeline bioreactor inside pipe wall method for cleaning of the present invention is as follows:
1, set cleaning magnetic spheres 1 in the pipeline Photoreactor, the concrete quantity of magnetic spheres is unrestricted, at least 1, also can be tens, has certain plasticity and magnetic, the size of each magnetic spheres 1 equates (pipeline that is bioreactor 4 adopts the gap to cooperate substantially with magnetic spheres 1, and magnetic spheres 1 just can be moved at the pipeline of bioreactor 4) substantially with the pipeline internal diameter of bioreactor 4.
The rigidity magnet can be for being attracted control device 2 by the magnetic line of force, can produce the material of attraction each other by magnetic spheres, as: Related product and materials such as magnet, the ore of inducing back band magnetic or metal.And plastic material refers to that various rubber, silica gel or foam etc. can wrap up magnet and have certain plastic shrink-down material.
2, one or more puts into pipeline bioreactor 4 with magnetic spheres 1, after disinfecting with pipeline bioreactor 4 and nutrient solution etc., and the little algae of inoculation in nutrient solution.
3, closing control switch 31, open switch I 9, switch I I 10, water pump 8 turns on the power, nutrient solution begins to flow under dynamic action, magnetic spheres enters in the pipeline of bioreactor 4 at random and carries out orientation and move with nutrient solution, also can be subjected to pipeline external control system system's manual adjustment and slide, distance is not concrete between magnetic spheres limits, magnetic spheres 1 constantly produces slight impact and friction with tube wall in the circulation moving process in Photoreactor pipeline 4, thereby the materials such as frustule that will paste on the transparent pipeline inwall rub in the liquid phase of the little algae of cultivation, pipeline bioreactor 4 is effectively cleared up and kept its high light transmittance.
4, after magnetic spheres 1 is finished 4 cleanings of pipeline bioreactor, arrive magnetic spheres occluding device 5 positions, magnetic spheres 1 is blocked, and nutrient solution enters the recovery of algae liquid, gas exchange and temperature control tower 6 smoothly by this magnetic spheres occluding device 5.
The magnetic spheres 1 of 5, being obstructed enters circulating line bypass 3 under the effect of gravity and magnetic attracting force, reclaim, be deposited in the upper side position of gauge tap 31.
6, close kinetic pump 8, open gauge tap 31, magnetic spheres 1 is fallen the downside of gauge tap 31; The closing control switch 31 then, restart kinetic pump 8, magnetic spheres 1 reenters in the pipeline bioreactor 4, produce friction with tube wall, the materials such as frustule that paste on the transparent pipeline inwall are rubbed in the liquid phase of the little algae of cultivation, its high light transmittance can be effectively cleaned out the attached cell on the pipeline bioreactor 4 etc. and be kept in circulation so repeatedly.
7, the algae liquid by magnetic spheres occluding device 5 enters the recovery of algae liquid, gas exchange and temperature control tower 6 smoothly, according to sensor 7 signal collected and programme-control processing, carry out oxygen parsing, carbon dioxide compensation and temperature exchange etc., reenter pipeline bioreactor 4 by kinetic pump 8 then, carry out the light autophyting growth.
8, magnetic spheres 1 enters pipeline bioreactor 4 by circulating line bypass 3, and therefore its path can avoid kinetic pump 8 to be subjected to mechanical damage without kinetic pump 8.
9, utilize the present invention to can be applicable in the incubation of dissimilar little algaes, improve culture efficiency.Pipeline bioreactor cultured microalgae or the adherent little algae that utilizes this method to remove, comprising coming from freshwater microalgae, becoming the little algae in the environment such as water or seawater, mainly is little algae etc. of the little algae of little algae aquaculture bait micro-algae, the little algae bioenergy of exploitation (as biodiesel etc.), high value added product and the industrial chemicals of wherein economically valuable.As bait micro-algae such as: flat algae, chlorella, chrysophyceae, grid algae, haematococcus, purple ball algae, snow algae or diatom and economic little algae of being rich in high added value, also comprise the little algae of the produce oil that is rich in grease.Therefore, the present invention is applied in aquatic feed, the exploitation of special bioactive ingredients and the little algae biodiesel raw material preparation process.
Embodiment: the cleaning technique method of the pipeline bioreactor of 5 tons of scales
At first, made up one group of pipeline bioreactor.Transparent material adopts the glass tube of 200cm length * 5.4cm internal diameter; At first, 25 glass tubes are connected into the long pipeline of 5000cm; Then, adopt and be arranged in parallel technology with 36 5000cm pipelines, the branch both sides are installed on the bracing frame, 18 pipelines of every side; Being arranged in the bracing frame pipelines on two sides is linked together by elbow respectively.Above-mentioned light-receiving tube road part forms 5 tons of pipeline bioreactors of volume together with gas exchange and temperature control tower etc.
As adopt routine techniques, this pipeline bioreactor is inoculated little algae (haematococcus swarm cell) and is cultivated after 7 days, a large amount of attachments (the sampling microscopy is mainly the not kinetocyte that is transformed by the haematococcus swarm cell) promptly appear on the tube wall, in any case change flow velocity, above-mentioned attached cell is difficult to remove all securely attached on the inner-walls of duct.Have no alternative but to abandon cultivate, the opening conduits coupling part is manually cleaned, and thoroughly cleans up and re-assemblies, and needs 3 workmans to spend two day time at least and just can finish.This method is difficult to realize Continuous Cultivation simultaneously, and culture efficiency is poor.
Equally, use the present invention, when above-mentioned pipeline bioreactor is inoculated little algae (haematococcus swarm cell), lay magnetic spheres.At least 1 of magnetic spheres, in the present embodiment, magnetic spheres is 5-15, about bioreactor internal diameter of the pipeline 5.4cm, the diameter of magnetic spheres is substantially about 5.4cm, the magnet diameter of magnetic spheres is about 2.0cm, and cultivating liquid specific gravity is 1.0, and the overall proportion of magnetic spheres is also about 1.0.
Utilize magnetic spheres irregularly to circulate the pipe blow-through inwall in light-receiving tube road part.Found that,, after magnetic spheres is passed through, almost all rub continued growth in the cultivation algae liquid phase attached to the attached cell on the pipeline and other material, and the basic full recovery of the light transmission of pipeline.In addition, owing to use the present invention, system still keeps closure, reduced the probability that system is polluted, therefore can continue to keep Continuous Cultivation, the cell homogeneity is improved, little algae photosynthetic efficiency and cell culture efficiency obviously strengthen, and cultured cells density further increases by 50%.
Claims (10)
1. the method for cleaning of a pipeline bioreactor inside pipe wall, it is characterized in that: between the inlet ductwork and export pipeline of pipeline bioreactor, the circulating line bypass is set, in the circulating line bypass, gauge tap is set, in the circulatory system of circulating line bypass and pipeline bioreactor formation, sets up at least one magnetic spheres;
Magnetic spheres is put into the pipeline bioreactor, nutrient solution begins to flow under dynamic action, magnetic spheres enters in the pipeline of bioreactor at random and carries out orientation and move with nutrient solution, perhaps be subjected to pipeline external control system system's manual adjustment and slide, magnetic spheres constantly produces slight impact and friction with tube wall in the circulation moving process in the Photoreactor pipeline, thereby the frustule material that will paste on the transparent pipeline inwall rubs in the liquid phase of the little algae of cultivation, the pipeline bioreactor is effectively cleared up and is kept its light transmission.
2. according to the method for cleaning of the described pipeline bioreactor of claim 1 inside pipe wall, it is characterized in that: the magnetic spheres occluding device is set on the export pipeline of pipeline bioreactor, and the magnetic spheres occluding device is positioned at the circulatory system outside of circulating line bypass and pipeline bioreactor formation;
Magnetic spheres arrives the occluding device position after finishing the cleaning of pipeline bioreactor, and magnetic spheres is blocked, and nutrient solution enters the recovery of algae liquid, gas exchange and temperature control tower smoothly by this magnetic spheres occluding device; The magnetic spheres of being obstructed enters the circulating line bypass under the effect of gravity and magnetic attracting force, reclaim, be deposited in the upper side position of gauge tap.
3. according to the method for cleaning of the described pipeline bioreactor of claim 2 inside pipe wall, it is characterized in that: the magnetic spheres occluding device adopts the network structure that can stop magnetic spheres to be passed through.
4. according to the method for cleaning of claim 1 or 2 described pipeline bioreactor inside pipe walls, it is characterized in that: the size of each magnetic spheres equates with the pipeline internal diameter of bioreactor substantially, the pipeline of bioreactor and magnetic spheres adopt matched in clearance, and magnetic spheres is just moved at the pipeline of bioreactor.
5. according to the method for cleaning of claim 1 or 2 described pipeline bioreactor inside pipe walls, it is characterized in that: magnetic spheres has the inside and outside double-layer structure that is provided with, inner can produce magnetic or by inducing the material of generation magnetic for rigidity, outside plastic material for wrapping up magnet, avoiding its crash response organ pipe wall, have shrinkage, the overall proportion of magnetic spheres equates with the proportion of micro algae culturing liquid or is bigger.
6. according to the method for cleaning of the described pipeline bioreactor of claim 5 inside pipe wall, it is characterized in that: the mesh aperture on the occluding device is less than whole magnetic spheres diameter, and the magnet diameter middle than magnetic spheres of the mesh aperture on the occluding device is little.
7. according to the method for cleaning of the described pipeline bioreactor of claim 1 inside pipe wall, it is characterized in that: attract control device in pipeline bioreactor peripheral hardware magnetic spheres, magnetic spheres attracts control device guiding magnetic spheres to enter the circulation bypass or rest on the somewhere.
8. according to the method for cleaning of the described pipeline bioreactor of claim 1 inside pipe wall, it is characterized in that: an end of circulating line bypass be connected pipeline bioreactor light accepting part divide with algae liquid reclaim, on the pipeline between gas exchange and the temperature control tower, magnetic spheres attraction control device is arranged at an end of described circulating line bypass; The other end of circulating line bypass is connected between pipeline bioreactor light accepting part branch and the kinetic pump, and the switching of this circulating line bypass is controlled by the ball-type gauge tap.
9. according to the method for cleaning of the described pipeline bioreactor of claim 1 inside pipe wall, it is characterized in that: kinetic pump, switch I, switch I I are set on the inlet ductwork of pipeline bioreactor, switch I, kinetic pump are positioned at a side of circulating line bypass in order, and switch I I is positioned at the opposite side of circulating line bypass;
Close kinetic pump, open gauge tap, magnetic spheres is fallen the downside of gauge tap; Closing control switch then, restart kinetic pump, magnetic spheres reenters in the pipeline bioreactor, produce friction with tube wall, the frustule material that pastes on the transparent pipeline inwall is rubbed in the liquid phase of the little algae of cultivation, circulation is so repeatedly effectively cleared up the attached cell on the pipeline bioreactor and is kept its light transmission.
10. according to the method for cleaning of the described pipeline bioreactor of claim 1 inside pipe wall, it is characterized in that: little algae comes from freshwater microalgae, becomes in water or the briny environment, comprises flat algae, chlorella, chrysophyceae, grid algae, haematococcus, purple ball algae, snow algae or diatom.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010105009269A CN102000679B (en) | 2010-09-30 | 2010-09-30 | Method for cleaning inner wall of pipeline type photobioreactor pipe |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010105009269A CN102000679B (en) | 2010-09-30 | 2010-09-30 | Method for cleaning inner wall of pipeline type photobioreactor pipe |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102000679A true CN102000679A (en) | 2011-04-06 |
| CN102000679B CN102000679B (en) | 2012-07-25 |
Family
ID=43808528
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010105009269A Active CN102000679B (en) | 2010-09-30 | 2010-09-30 | Method for cleaning inner wall of pipeline type photobioreactor pipe |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102000679B (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106906118A (en) * | 2015-12-22 | 2017-06-30 | 国家开发投资公司 | The cleaning device and bioreactor of bioreactor |
| CN108611262A (en) * | 2018-04-17 | 2018-10-02 | 曾坚 | Self-cleaning inner tubal wall duct type bioreactor |
| CN108893404A (en) * | 2018-07-19 | 2018-11-27 | 佛山市宝粤美科技有限公司 | A kind of sampler and its application method based on pipeline cultivation algae |
| CN110423681A (en) * | 2019-09-17 | 2019-11-08 | 肖卫武 | A kind of photosynthetic microorganism culture production system |
| CN113403197A (en) * | 2021-05-14 | 2021-09-17 | 江苏大学 | Photo-biological reaction system and method for culturing microalgae by magnetic field coupling |
| CN113941572A (en) * | 2021-09-27 | 2022-01-18 | 朱淮兵 | Steel pipe circulating cleaning method and equipment |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106906116B (en) * | 2015-12-22 | 2020-04-14 | 国投生物科技投资有限公司 | Cleaning device of tube bank type photobioreactor and photobioreactor |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4234993A (en) * | 1979-05-30 | 1980-11-25 | Kintner Edwin K | Condenser cleaning system using sponge balls |
| EP0026261A1 (en) * | 1979-08-14 | 1981-04-08 | Werner Borchert | Device for separating cleaning bodies from a fluid flowing out of a shell-and-tube heat exchanger |
| JP2002210428A (en) * | 2001-01-19 | 2002-07-30 | Chubu Electric Power Co Inc | Ball for cleaning inside of pipe, and cleaning pig for inspecting passage of pipe |
| CN2580386Y (en) * | 2002-09-28 | 2003-10-15 | 倪永刚 | On-line automatic cleaning device for condensator heat exchange tube inner wall |
| JP2003305423A (en) * | 2002-04-15 | 2003-10-28 | Kenji Kuwajima | Pipe cleaning tool and its manufacturing method |
| CN1778482A (en) * | 2004-11-25 | 2006-05-31 | 杜家财 | Cleaner of electric water heater |
| CN2906528Y (en) * | 2006-04-27 | 2007-05-30 | 肖继恺 | Solar heat collecting pipe cleaning device |
| TW201027026A (en) * | 2009-01-13 | 2010-07-16 | zheng-fa Zhou | Heat-exchange cleaning system |
-
2010
- 2010-09-30 CN CN2010105009269A patent/CN102000679B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4234993A (en) * | 1979-05-30 | 1980-11-25 | Kintner Edwin K | Condenser cleaning system using sponge balls |
| EP0026261A1 (en) * | 1979-08-14 | 1981-04-08 | Werner Borchert | Device for separating cleaning bodies from a fluid flowing out of a shell-and-tube heat exchanger |
| JP2002210428A (en) * | 2001-01-19 | 2002-07-30 | Chubu Electric Power Co Inc | Ball for cleaning inside of pipe, and cleaning pig for inspecting passage of pipe |
| JP2003305423A (en) * | 2002-04-15 | 2003-10-28 | Kenji Kuwajima | Pipe cleaning tool and its manufacturing method |
| CN2580386Y (en) * | 2002-09-28 | 2003-10-15 | 倪永刚 | On-line automatic cleaning device for condensator heat exchange tube inner wall |
| CN1778482A (en) * | 2004-11-25 | 2006-05-31 | 杜家财 | Cleaner of electric water heater |
| CN2906528Y (en) * | 2006-04-27 | 2007-05-30 | 肖继恺 | Solar heat collecting pipe cleaning device |
| TW201027026A (en) * | 2009-01-13 | 2010-07-16 | zheng-fa Zhou | Heat-exchange cleaning system |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106906118A (en) * | 2015-12-22 | 2017-06-30 | 国家开发投资公司 | The cleaning device and bioreactor of bioreactor |
| CN106906118B (en) * | 2015-12-22 | 2019-07-19 | 国投生物科技投资有限公司 | The cleaning device and bioreactor of bioreactor |
| CN108611262A (en) * | 2018-04-17 | 2018-10-02 | 曾坚 | Self-cleaning inner tubal wall duct type bioreactor |
| CN108893404A (en) * | 2018-07-19 | 2018-11-27 | 佛山市宝粤美科技有限公司 | A kind of sampler and its application method based on pipeline cultivation algae |
| CN110423681A (en) * | 2019-09-17 | 2019-11-08 | 肖卫武 | A kind of photosynthetic microorganism culture production system |
| CN113403197A (en) * | 2021-05-14 | 2021-09-17 | 江苏大学 | Photo-biological reaction system and method for culturing microalgae by magnetic field coupling |
| CN113403197B (en) * | 2021-05-14 | 2024-04-09 | 江苏大学 | Photo-biological reaction system and method for culturing microalgae by utilizing magnetic field coupling |
| CN113941572A (en) * | 2021-09-27 | 2022-01-18 | 朱淮兵 | Steel pipe circulating cleaning method and equipment |
| CN113941572B (en) * | 2021-09-27 | 2022-10-25 | 宁波科尼管洁净科技有限公司 | Steel pipe circulating cleaning method and equipment |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102000679B (en) | 2012-07-25 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102000679B (en) | Method for cleaning inner wall of pipeline type photobioreactor pipe | |
| Xu et al. | Development of integrated culture systems and harvesting methods for improved algal biomass productivity and wastewater resource recovery–a review | |
| CN102134553B (en) | Tubular photobioreactor and system and method for culturing microalgae cells | |
| KR101148194B1 (en) | Photobioreactor with Transparent Film | |
| CN102206570B (en) | Apparatus and cultivating method for scaled cultivation of microalgae | |
| Huang et al. | Improvement on light penetrability and microalgae biomass production by periodically pre-harvesting Chlorella vulgaris cells with culture medium recycling | |
| CN102382754B (en) | Optical fiber photobioreactor for making full use of 'flash effect' of microalgae | |
| CN1213139C (en) | Photosynthesis bioreactor system for industrial production of micro algae | |
| CN102583767B (en) | System for treating sewage and producing biological oil by using microalgae and method | |
| CN102329720B (en) | Photobioreactor capable of realizing high-efficiency carbon dioxide immobilization | |
| CN102382755A (en) | Microalgae culturing device and microalgae culturing method | |
| CN103289888A (en) | Inserting-plate type microalgae semi-dry solid adherent culture device | |
| CN105002094A (en) | Method for semi-continuously culturing marine microalgae through solar greenhouse | |
| Mahmoud et al. | Closed photobioreactor for microalgae biomass production under indoor growth conditions | |
| CN201729830U (en) | Sealed pipeline culturing device for bait microalgae | |
| CN203462044U (en) | Cylindrical photobioreactor for bait microalgae cultivation | |
| CN101906380B (en) | Seal type pipeline culture device of bait microalgae and microalgae pipeline culture method | |
| CN102296025B (en) | Photobioreactor capable of realizing internal and external circulation of alga liquid | |
| CN107641594B (en) | Differential pressure type open pipeline bioreactor for microalgae culture | |
| CN201381319Y (en) | Optical bioreactor for nursing microalgae | |
| CN106635768B (en) | Biological microalgae photosynthetic reactor and its application method | |
| CN102373151A (en) | Photobioreactor and photobiology culture system | |
| CN102373150A (en) | Photobioreactor and photobiology culture system | |
| CN102373149A (en) | Photobioreactor, its preparation method, photo-biological culture system and method | |
| CN202881245U (en) | Photo-bioreactor |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |