CN102000679B - Method for cleaning inner wall of pipeline type photobioreactor pipe - Google Patents
Method for cleaning inner wall of pipeline type photobioreactor pipe Download PDFInfo
- Publication number
- CN102000679B CN102000679B CN2010105009269A CN201010500926A CN102000679B CN 102000679 B CN102000679 B CN 102000679B CN 2010105009269 A CN2010105009269 A CN 2010105009269A CN 201010500926 A CN201010500926 A CN 201010500926A CN 102000679 B CN102000679 B CN 102000679B
- Authority
- CN
- China
- Prior art keywords
- pipeline
- bioreactor
- magnetic spheres
- algae
- magnetic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000000034 method Methods 0.000 title claims abstract description 45
- 238000004140 cleaning Methods 0.000 title claims abstract description 36
- 241000195493 Cryptophyta Species 0.000 claims abstract description 101
- 239000000463 material Substances 0.000 claims abstract description 25
- 238000012258 culturing Methods 0.000 claims abstract description 12
- 230000008569 process Effects 0.000 claims abstract description 12
- 230000005540 biological transmission Effects 0.000 claims abstract description 10
- 230000000694 effects Effects 0.000 claims abstract description 10
- 239000007791 liquid phase Substances 0.000 claims abstract description 10
- 239000007788 liquid Substances 0.000 claims description 34
- 238000011084 recovery Methods 0.000 claims description 16
- 235000015097 nutrients Nutrition 0.000 claims description 11
- 239000004033 plastic Substances 0.000 claims description 9
- 229920003023 plastic Polymers 0.000 claims description 9
- 241000168525 Haematococcus Species 0.000 claims description 6
- 230000015572 biosynthetic process Effects 0.000 claims description 6
- 230000005484 gravity Effects 0.000 claims description 5
- 230000002093 peripheral effect Effects 0.000 claims description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- 230000001939 inductive effect Effects 0.000 claims description 4
- 210000000056 organ Anatomy 0.000 claims description 4
- 241000195649 Chlorella <Chlorellales> Species 0.000 claims description 3
- 241000206751 Chrysophyceae Species 0.000 claims description 3
- 230000009471 action Effects 0.000 claims description 3
- 239000013505 freshwater Substances 0.000 claims description 3
- 230000004044 response Effects 0.000 claims description 3
- 150000003839 salts Chemical class 0.000 claims description 2
- 238000004113 cell culture Methods 0.000 abstract description 3
- 230000026058 directional locomotion Effects 0.000 abstract 1
- 239000000126 substance Substances 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 description 31
- 230000001464 adherent effect Effects 0.000 description 16
- 239000007789 gas Substances 0.000 description 15
- 238000011161 development Methods 0.000 description 9
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 8
- 239000003225 biodiesel Substances 0.000 description 5
- 230000006378 damage Effects 0.000 description 5
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 4
- 229910002092 carbon dioxide Inorganic materials 0.000 description 4
- 239000001569 carbon dioxide Substances 0.000 description 4
- 238000005755 formation reaction Methods 0.000 description 4
- 230000008676 import Effects 0.000 description 4
- 230000001965 increasing effect Effects 0.000 description 4
- 229910052760 oxygen Inorganic materials 0.000 description 4
- 239000001301 oxygen Substances 0.000 description 4
- 230000000243 photosynthetic effect Effects 0.000 description 4
- 239000011521 glass Substances 0.000 description 3
- 238000002834 transmittance Methods 0.000 description 3
- 208000027418 Wounds and injury Diseases 0.000 description 2
- 238000009360 aquaculture Methods 0.000 description 2
- 244000144974 aquaculture Species 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 230000000975 bioactive effect Effects 0.000 description 2
- 230000030833 cell death Effects 0.000 description 2
- 230000008878 coupling Effects 0.000 description 2
- 238000010168 coupling process Methods 0.000 description 2
- 238000005859 coupling reaction Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000004519 grease Substances 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 208000014674 injury Diseases 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000003208 petroleum Substances 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 235000016425 Arthrospira platensis Nutrition 0.000 description 1
- 240000002900 Arthrospira platensis Species 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 241001262079 Cutleriales Species 0.000 description 1
- 229920005479 Lucite® Polymers 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 241000719329 Trentepohlia Species 0.000 description 1
- 239000002156 adsorbate Substances 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 238000000429 assembly Methods 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000006835 compression Effects 0.000 description 1
- 238000007906 compression Methods 0.000 description 1
- 210000004748 cultured cell Anatomy 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 230000000249 desinfective effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000002283 diesel fuel Substances 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000006260 foam Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000013467 fragmentation Methods 0.000 description 1
- 238000006062 fragmentation reaction Methods 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 239000003317 industrial substance Substances 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 230000037353 metabolic pathway Effects 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 238000000386 microscopy Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 230000029264 phototaxis Effects 0.000 description 1
- 239000004926 polymethyl methacrylate Substances 0.000 description 1
- 230000001172 regenerating effect Effects 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 239000005060 rubber Substances 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 239000013535 sea water Substances 0.000 description 1
- 238000004062 sedimentation Methods 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- 229940082787 spirulina Drugs 0.000 description 1
- 239000012780 transparent material Substances 0.000 description 1
Images
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention belongs to the biologic field of micro algae, relating to a method for cleaning inner wall of pipeline type photobioreactor pipe in micro algae culture. Magnetic sphere is placed into a pipeline type photobioreactor, culture solution flows when being pushed by power, the magnetic sphere randomly enters into the pipeline of photobioreactor along with the culture solution and does directional movement or slides under manual regulation of a control system outside the pipeline, and the magnetic sphere continuously slightly collides and rubs pipe wall in circulated moving process in the photobioreactor pipeline, so that algae cell material adhered onto transparent pipeline inner wall is rubbed into liquid phase used for culturing micro algae, the pipeline photobioreactor pipeline is effectively cleaned and light transmission thereof is maintained. The invention can effectively clean cells into algae liquid phase at initial stage of adherence, or substance such as cell adhered to the inner wall of the pipeline type photobioreactor pipe is removed, restriction of micro algae cell adherence to micro algae cell culture is overcome, and good light transmission and culture effect of photobioreactor are maintained.
Description
Technical field
The invention belongs to little algae biological field, relate to a kind of method for cleaning of pipeline bioreactor inside pipe wall of little algae cultivation.
Background technology
Little algae is unique bait of aquatic livestock whole growth process in the aquatic ecosystem (or specific stage of development) or the bait of food organisms as the important component part of primary productive force in the biosphere.Simultaneously; Little algae is near floristic 50%; Has the diversity of going out, complexity and particularity at aspects such as germplasm, ecological distribution, hereditary information, biochemical composition, metabolic pathways; Further determined nutrition and pharmacy value that it is potential, the mankind possibly therefrom develop the special high added value bioactive ingredients of a large amount of structures.
The energy be pillar of economy also be the motive force of social development, determining modern civilization progress degree.Be accompanied by global economy rapid growth and urbanization process and accelerate, in the world the petroleum-based energy increasing demand is increased, traditional energies such as while oil all have non-renewable, exhausted because of a large amount of exploitations at last, so the petroleum-based energy shortage problem becomes increasingly conspicuous.In recent years, be the new force of regenerative resource in the world as the biodiesel of one of substitute of fossil energy, development is swift and violent, range of application constantly enlarges, and proportion grows steadily in diesel oil market.Wherein little algae has that photosynthetic efficiency height, oil content are high, growth cycle is short, the grease productivity per unit is high, help reducing the gentle unique advantage such as environment pressure of separating of carbon emission with the absorbing carbon dioxide coupling, therefore develop little algae bioenergy (biodiesel etc.) be considered to development potentiality huge, most possibly substitute the bioenergy of oil.
Yet there is not a large amount of ready-made little algae resource that can directly collect processing in nature.Exploitation supplies the required little algae resource of human social development, at first wants the little algae raw material of scale engineering cultivating and producing.In the world, mainly contain open pond and 2 types of modes of closed photobioreactor before the little Cutleriales of micro-algae large-scale ARTIFICIAL CULTURE:
Open cultivation (like circulation runway pond) is simple relatively, investment is low.But this mode condition of culture changes greatly, and little algae productive rate is low, and has serious biological pollution.Any natural enemy biological (like protozoan) and other algae that comes from water source, air, fertilizer, instrument and operation etc. all very easily invaded pollution, usually causes whole cultivation utter failure, directly limited the little algae of influence and cultivated and development of resources.Have only little algae that only a few can grow in extreme environment (as in high pH environment fast the spirulina of growth and in high salinity, become the salt algaes of sociales) can utilize this mode culture successful.
Though the closed photo bioreactor investment cost is relatively large, owing to can regulate and control multinomial culture parameters, be easier to control biological pollution, the productive rate of culturing microalgae is higher, therefore becomes the important trend of this area research and development in recent years.The closed photo bioreactor that has been used for laboratory and lab scale research (working volume from several be raised to several hectolitres) at present is a lot, and pot type bioreactor, dull and stereotyped bioreactor, column bioreactor, pipeline (vertical, parallel, spiral) bioreactor are arranged.But several ton to up to a hundred ton the closed photo bioreactor of amplification culture sizable application in produce but seldom veritably.Cause the related reason of this situation a lot; (its resistance to compression and high temperature resistant property are very low not only to comprise the physicochemical property of translucent material (glass, lucite and plastics); Be easy to fragmentation and with thermal deformation even fusing etc.) restriction; And need effectively compensate carbon dioxide-depleted, efficiently resolve too high dissolved oxygen and avoid high oxygen injury, and when volume of culture increases, keep the reactor body surface area and do not descend degradation.At present, the development of pipeline bioreactor can address the above problem to a certain extent, becomes one of major technique means that increase the bioreactor volume gradually.
It may be noted that bioreactor (particularly pipeline bioreactor) is inner very easily takes place and the cell attachment phenomenon occurs adhering to of microalgae cell etc.How effectively to remove these attachments, ensure that the light transmission of bioreactor is the problem that bothers very much with keeping high culture efficiency, this is not had good solution in the past.Along with little algae biotechnology and little algae industry development, cleaning and remove adherent little algae has become little algae biological technical field research focus at home and abroad, in development and Application of Large pipeline bioreactor, is badly in need of solving.
Little Trentepohlia has characteristics such as phototaxis, photophobism, adherent growth concurrently in photoautotroph, and in little algae incubation, cell usually is easy to paste reactor tube walls etc. and locates.The adherent degree of microalgae cell is not only relevant with different microalgae self biological characteristics, and is also relevant with the moving speed of algae flow.
In pipeline, pipeline zone line flow velocity is the fastest, and slow more near the nearlyer flow velocity of tube wall, is close to zero at the regional flow velocity of abuts, and this zone is the dead angle of bioreactor.In any bioreactor, all there is above-mentioned dead angle area (dead angle also comprises the positions such as turning in the reactor) inevitably.Generally speaking, algae liquid overall flow speed is fast more, and is just few more near the dead angle area of low flow velocity of tube wall even zero flow velocity; Otherwise the moving speed of algae flow is slower, near the low flow velocity of tube wall and zero flow velocity dead angle area the more.
At dead angle area, algae liquid is mixing fully, is prone to take place sedimentation or adherent growth.In the pipeline bioreactor,, always exist near low flow velocity of tube wall even zero flow velocity zone, so adherent situation takes place just unavoidably in the cultured microalgae cell regardless of algae liquid overall flow speed.
In a single day microalgae cell takes place adherent, produces vicious circle and serial adverse effect below the formation, and the cell culture effect is constantly descended, even causes whole cultivating system collapse.At first; Microalgae cell takes place adherent behind reactor wall, causes the reactor material rough surface, has increased fluid resistance; Reduced the algae flow velocity; Further increase dead angle quantity in the bioreactor, made cell that adherent phenomenon take place more easily, the increasing vicious circle of cell attachment degree; Secondly; After microalgae cell is adherent; Formed little algae spot (or little algae thin layer) also is at a distance from luminous point (or even at a distance from photosphere), influences the translucidus of bioreactor to some extent, has hindered light and has got into bioreactor inside; So insufficient light that inner microalgae cell absorbs, the microalgae cell photosynthetic efficiency descends; The 3rd, be in the high light state all the time near the attached cell of pipeline, light supersaturation and light injury takes place causes this part cell photosynthetic efficiency to descend, even cell death takes place.In addition, if after the attached cell death, frond takes place to rot, and also influences little algae product quality.
Summary of the invention
In large-scale closed conduit bioreactor application process, the adherent problem of microalgae cell unavoidably appears, takes place for reducing the adherent phenomenon of little algae, keep bioreactor light transmission and culture effect.The object of the present invention is to provide a kind of method for cleaning of pipeline bioreactor inside pipe wall of little algae cultivation; Can just it effectively be cleaned in the algae liquid phase at the cell attachment early period of origination effectively; The materials such as attached cell that maybe will stick on the pipeline bioreactor inside pipe wall are removed; Overcome the adherent restriction that microalgae cell is cultivated of microalgae cell, keep bioreactor good light transmittance and culture effect.
The present invention solves and to be adsorbed on the scheme that the adsorbates such as adherent microalgae cell on the pipeline bioreactor inside pipe wall take and to be:
A kind of method for cleaning of pipeline bioreactor inside pipe wall; Between the inlet ductwork and export pipeline of pipeline bioreactor; The circulating line bypass is set; In the circulating line bypass, gauge tap is set, in the circulatory system of circulating line bypass and pipeline bioreactor formation, sets up at least one magnetic spheres;
Magnetic spheres is put into the pipeline bioreactor; Nutrient solution begins to flow under dynamic action; Magnetic spheres gets in the pipeline of bioreactor at random and carries out orientation and move with nutrient solution; Perhaps receive pipeline outer control system's manual adjustment and slide; Magnetic spheres constantly produces slight impact and friction with tube wall in the circulation moving process in the Photoreactor pipeline, thereby the frustule material that will paste on the transparent pipeline inwall rubs in the liquid phase of culturing microalgae, and the pipeline bioreactor is effectively cleared up and kept its light transmission.
The method for cleaning of described pipeline bioreactor inside pipe wall; The magnetic spheres occluding device is set on the export pipeline of pipeline bioreactor, and the magnetic spheres occluding device is positioned at the circulatory system outside of circulating line bypass and pipeline bioreactor formation;
Magnetic spheres arrives the occluding device position after accomplishing the cleaning of pipeline bioreactor, and magnetic spheres is blocked, and nutrient solution gets into the recovery of algae liquid, Gas Exchange and temperature control tower smoothly through this magnetic spheres occluding device; The magnetic spheres of being obstructed gets into the circulating line bypass under the effect of gravity and magnetic attracting force, reclaim, be deposited in the upper side position of gauge tap.
The method for cleaning of described pipeline bioreactor inside pipe wall, the magnetic spheres occluding device adopts the network structure that can stop magnetic spheres to be passed through.
The method for cleaning of described pipeline bioreactor inside pipe wall; The size of each magnetic spheres equates with the pipeline internal diameter of bioreactor basically; The pipeline of bioreactor and magnetic spheres adopt matched in clearance, and magnetic spheres is just moved at the pipeline of bioreactor.
The method for cleaning of described pipeline bioreactor inside pipe wall; Magnetic spheres has the inside and outside double-layer structure that is provided with; Inner can produce magnetic or through inducing the material of generation magnetic for rigidity; Outside plastic material for wrapping up magnet, avoiding its crash response organ pipe wall, have certain shrinkage, the overall proportion of magnetic spheres equates with the proportion of micro algae culturing liquid or is bigger.
The method for cleaning of described pipeline bioreactor inside pipe wall, the mesh aperture on the occluding device be less than whole magnetic spheres diameter, and the magnet diameter middle than magnetic spheres of the mesh aperture on the occluding device is little.
The method for cleaning of described pipeline bioreactor inside pipe wall attracts control device in pipeline bioreactor peripheral hardware magnetic spheres, and magnetic spheres attracts control device guiding magnetic spheres to get into the circulation bypass or rest on the somewhere.
The method for cleaning of described pipeline bioreactor inside pipe wall; One end of circulating line bypass is connected on the pipeline between pipeline bioreactor light accepting part branch and the recovery of algae liquid, Gas Exchange and the temperature control tower, and magnetic spheres attracts control device to be arranged at an end of said circulating line bypass; The other end of circulating line bypass is connected between pipeline bioreactor light accepting part branch and the kinetic pump, and the switching of this circulating line bypass is controlled by the ball-type gauge tap.
The method for cleaning of described pipeline bioreactor inside pipe wall; Kinetic pump, switch I, switch I I are set on the inlet ductwork of pipeline bioreactor; Switch I, kinetic pump are positioned at a side of circulating line bypass in order, and switch I I is positioned at the opposite side of circulating line bypass;
Close kinetic pump, open gauge tap, magnetic spheres is fallen the downside of gauge tap; Closing control switch then; Restart kinetic pump; Magnetic spheres gets in the pipeline bioreactor again, produces friction with tube wall, and the frustule material that pastes on the transparent pipeline inwall is rubbed in the liquid phase of culturing microalgae; Circulation is so repeatedly effectively cleared up the attached cell on the pipeline bioreactor and is kept its light transmission.
The method for cleaning of described pipeline bioreactor inside pipe wall, little algae come from freshwater microalgae, become in water or the briny environment, comprise flat algae, chlorella, chrysophyceae, grid algae, haematococcus, purple ball algae, snow algae or diatom etc.
Advantage of the present invention is:
1, magnetic spheres of the present invention circulates in the pipeline bioreactor by current and moves, and the magnetic spheres size equates basically that with internal diameter of the pipeline therefore magnetic spheres and inner-walls of duct produce slight impact and friction in moving process.Therefore, can effectively remove, thereby ensure good light permeability and the high culture efficiency in the bioreactor operation process attached to adherent microalgae cell on the inside pipe wall and other material.
2, magnetic spheres of the present invention has plasticity, has avoided hard collision, cut equivalent damage to pipeline, therefore the pipeline bioreactor is played the certain protection effect.
3, the present invention is provided with magnetic spheres occluding device and circulation bypass and gauge tap; Realization cultivation algae liquid separates with magnetic spheres; Under the effect of kinetic pump etc.; Not only keep functions such as the normal algae flow of cultivating system is moving, Gas Exchange (oxygen is resolved with carbon dioxide and compensated), exchange heat, and magnetic spheres need not pass through kinetic pump in pipe blow-through inwall process, thereby avoid kinetic pump generation mechanical damage.
4, magnetic spheres of the present invention can recycle in the closed conduit bioreactor for a long time, has reduced the biological pollution that bioreactor takes place in opening procedure repeatedly.
5, magnetic spheres making of the present invention is simple, cheap, and operation is simple, production application is very economical, has reduced artificial cleaning difficulty of pipeline bioreactor and the high problem of cost, therefore has very big production application and is worth.
6, therefore the inventive method can keep its cultivation high efficiency of light transmission and guarantee of pipeline bioreactor for microalgae cell and other attachment of effective removal adherent growth in the closed conduit bioreactor provide possibility.Be of value to scale light autotrophy culturing microalgae cell, aquaculture bait, the little algae germplasm of high-purity be provided and produce little algae bioenergy (like biodiesel etc.) and the little algae product of high added value.
Description of drawings
Fig. 1 is pipeline photo-bioreactor system of the present invention and inside pipe wall cleaning signal pattern diagram.
Among the figure: 1, magnetic spheres; 1a, plastic material; 1b, magnet; 2, magnetic spheres attracts control device; 3, circulating line bypass; 31 gauge taps; 4, pipeline bioreactor; 5, magnetic spheres occluding device; 6, the recovery of algae liquid, Gas Exchange and temperature control tower; 7, sensor; 8, kinetic pump; 9, switch I; 10, switch I I.
The specific embodiment
The present invention's design is: at pipeline bioreactor set inside cleaning plant, and at the pipeline peripheral controller, but setting manual control switch's circulating line bypass on pipe-line system.
As shown in Figure 1; The pipeline bioreactor system mainly comprises: the cleaning magnetic spheres 1 of pipeline bioreactor set inside, attract control device 2, the circulating line bypass 3, pipeline bioreactor 4 newly-increased on the pipe-line system, magnetic spheres occluding device 5, the recovery of algae liquid, Gas Exchange and temperature control tower 6, various kinds of sensors 7, kinetic pump 8, switch I 9, the switch I I10 in pipeline optical-biological reaction organ pipe etc. in the magnetic spheres of pipeline bioreactor peripheral hardware, concrete structure is following:
The algae liquid outlet of the recovery of algae liquid, Gas Exchange and temperature control tower 6 is connected the algae liquid import of pipeline bioreactor 4 through pipeline; The algae liquid import of the recovery of algae liquid, Gas Exchange and temperature control tower 6 exports through the algae liquid that pipeline is connected pipeline bioreactor 4, in the recovery of algae liquid, Gas Exchange and temperature control tower 6, various kinds of sensors 7 is set.
Different with prior art is: between the inlet ductwork and export pipeline of pipeline bioreactor 4; The circulating line bypass is set; Promptly between two pipelines that the recovery of algae liquid, Gas Exchange and temperature control tower 6 communicate with pipeline bioreactor 4, increase circulating line bypass 3, gauge tap 31 is set in circulating line bypass 3; In the circulatory system that circulating line bypass 3 and pipeline bioreactor 4 constitute, set up the magnetic spheres 1 of at least one cleaning usefulness.
On the pipeline (being inlet ductwork) between the recovery of algae liquid, Gas Exchange and outlet of temperature control tower 6 algae liquid and the 4 algae liquid imports of pipeline bioreactor, kinetic pump 8, switch I 9, switch I I 10 are set; Switch I 9, kinetic pump 8 are positioned at a side (circulatory system outside that circulating line bypass and pipeline bioreactor constitute) of circulating line bypass 3 in order; Switch I I 10 is arranged in the opposite side (being positioned at the circulatory system that circulating line bypass and pipeline bioreactor constitute) of circulating line bypass 3, attracts control device 2 in pipeline bioreactor 4 peripheral hardware magnetic spheres.On the pipeline (being export pipeline) between the recovery of algae liquid, Gas Exchange and temperature control tower 6 algae liquid imports and the outlet of pipeline bioreactor 4 algae liquid, magnetic spheres occluding device 5 is set, magnetic spheres occluding device 5 is positioned at the circulatory system outside of circulating line bypass 3 and pipeline bioreactor 4 formations.
One end of this circulating line bypass 3 is connected on the pipeline between pipeline bioreactor 4 light accepting parts branch and the recovery of algae liquid, Gas Exchange and the temperature control tower 6, and magnetic spheres attracts control device 2 to be arranged at an end of said circulating line bypass 3.The other end of circulating line bypass 3 is connected between pipeline bioreactor 4 light accepting parts branch and the kinetic pump 8, and the switching of this circulating line bypass 3 is controlled by the ball-type gauge tap.
Among the present invention, magnetic spheres attracts control device 2 to refer to that all can produce the magnetic line of force, can attract object each other, as: magnet or electromagnetic coil.
Among the present invention, magnetic spheres occluding device 5 is adopted as network structure, has a lot of mesh on the device, and this occluding device does not allow magnetic spheres to pass through, but allows algae flow mistake.Mesh aperture on the occluding device, its size be not only less than whole magnetic spheres diameter, and also littler than the magnet diameter in the middle of the magnetic spheres 1.
As shown in Figure 1, the detailed process of pipeline bioreactor inside pipe wall method for cleaning of the present invention is following:
1, set cleaning magnetic spheres 1 in the pipeline Photoreactor; The concrete quantity of magnetic spheres is unrestricted; At least 1 can be tens also, has certain plasticity and magnetic; The size of each magnetic spheres 1 equates (pipeline that is bioreactor 4 cooperates with magnetic spheres 1 employing gap basically, and magnetic spheres 1 just can be moved at the pipeline of bioreactor 4) basically with the pipeline internal diameter of bioreactor 4.
The rigidity magnet can be for being attracted control device 2 through the magnetic line of force, can produce the material of attraction each other by magnetic spheres, as: Related product and materials such as magnet, the ore of inducing back band magnetic or metal.And plastic material refers to that various rubber, silica gel or foam etc. can wrap up magnet and have certain plastic shrink-down material.
2, one or more puts into pipeline bioreactor 4 with magnetic spheres 1, after disinfecting with pipeline bioreactor 4 and nutrient solution etc., and the little algae of inoculation in nutrient solution.
3, the closing control switch 31; Open switch I 9, switch I I 10, the water pump 8 that turns on the power, nutrient solution begins to flow under dynamic action; Magnetic spheres gets in the pipeline of bioreactor 4 at random and carries out orientation and move with nutrient solution; Also can receive pipeline outer control system's manual adjustment and slide, distance is not concrete between magnetic spheres limits, magnetic spheres 1 Photoreactor pipeline 4 in the circulation moving process constantly with tube wall generation slight impact and friction; Thereby the materials such as frustule that will paste on the transparent pipeline inwall rub in the liquid phase of culturing microalgae, and pipeline bioreactor 4 is effectively cleared up and kept its high light transmittance.
4, after magnetic spheres 1 is accomplished 4 cleanings of pipeline bioreactor, arrive magnetic spheres occluding device 5 positions, magnetic spheres 1 is blocked, and nutrient solution gets into the recovery of algae liquid, Gas Exchange and temperature control tower 6 smoothly through this magnetic spheres occluding device 5.
The magnetic spheres 1 of 5, being obstructed gets into circulating line bypass 3 under the effect of gravity and magnetic attracting force, reclaim, be deposited in the upper side position of gauge tap 31.
6, close kinetic pump 8, open gauge tap 31, magnetic spheres 1 is fallen the downside of gauge tap 31; The closing control switch 31 then; Restart kinetic pump 8; Magnetic spheres 1 gets in the pipeline bioreactor 4 again, produces friction with tube wall, and the materials such as frustule that paste on the transparent pipeline inwall are rubbed in the liquid phase of culturing microalgae; Circulation so repeatedly can effectively be cleaned out the attached cell on the pipeline bioreactor 4 etc. and keeps its high light transmittance.
7, the algae liquid through magnetic spheres occluding device 5 gets into the recovery of algae liquid, Gas Exchange and temperature control tower 6 smoothly; According to sensor 7 signal collected and programme-control processing; Carry out oxygen parsing, carbon dioxide compensation and temperature exchange etc.; Again get into pipeline bioreactor 4 through kinetic pump 8 then, carry out the light autophyting growth.
8, magnetic spheres 1 gets into pipeline bioreactor 4 through circulating line bypass 3, and therefore its path can avoid kinetic pump 8 to receive mechanical damage without kinetic pump 8.
9, utilize the present invention to can be applicable in the incubation of dissimilar little algaes, improve culture efficiency.Pipeline bioreactor cultured microalgae or the adherent little algae that utilizes this method to remove; Comprising coming from freshwater microalgae, becoming the little algae in the environment such as water or seawater, mainly is little algae etc. of the little algae of little algae aquaculture bait micro-algae, the little algae bioenergy of exploitation (like biodiesel etc.), high value added product and the industrial chemicals of wherein economically valuable.Like bait micro-algae such as: flat algae, chlorella, chrysophyceae, grid algae, haematococcus, purple ball algae, snow algae or diatom and economic little algae of being rich in high added value, also comprise the little algae of the produce oil that is rich in grease.Therefore, the present invention is applied in aquatic feed, special bioactive ingredients is developed and little algae biodiesel raw material prepares in the process.
Embodiment: the cleaning technique method of the pipeline bioreactor of 5 tons of scales
At first, made up one group of pipeline bioreactor.Transparent material adopts the glass tube of 200cm length * 5.4cm internal diameter; At first, 25 glass tubes are connected into the long pipeline of 5000cm; Then, adopt the technology of being arranged in parallel with 36 5000cm pipelines, the branch both sides are installed on the bracing frame, 18 pipelines of every side; Being arranged in the bracing frame pipelines on two sides is linked together by elbow respectively.Above-mentioned light-receiving tube road part forms 5 tons of pipeline bioreactors of volume together with Gas Exchange and temperature control tower etc.
As adopt routine techniques; This pipeline bioreactor is inoculated little algae (haematococcus swarm cell) and is cultivated after 7 days; A large amount of attachments (the sampling microscopy is mainly the not kinetocyte that is transformed by the haematococcus swarm cell) promptly appear on the tube wall; In any case change flow velocity, above-mentioned attached cell all securely attached on the inner-walls of duct, are difficult to remove.Have no alternative but to abandon cultivate, the opening conduits coupling part is carried out manual work and is cleaned, and thoroughly cleans up and re-assemblies, and 3 workmans of needs spend two day time and could accomplish at least.This method is difficult to realize Continuous Cultivation simultaneously, and culture efficiency is poor.
Equally, use the present invention, when above-mentioned pipeline bioreactor is inoculated little algae (haematococcus swarm cell), lay magnetic spheres.At least 1 of magnetic spheres; In the present embodiment, magnetic spheres is 5-15, about bioreactor internal diameter of the pipeline 5.4cm; The diameter of magnetic spheres is basically about 5.4cm; The magnet diameter of magnetic spheres is about 2.0cm, and cultivating liquid specific gravity is 1.0, and the overall proportion of magnetic spheres is also about 1.0.
Irregularly part circulates the pipe blow-through inwall in the light-receiving tube road to utilize magnetic spheres.The result finds, attached to the attached cell on the pipeline and other material, after magnetic spheres is passed through, almost all rubs continued growth in the cultivation algae liquid phase, and the basic full recovery of the light transmission of pipeline.In addition, owing to use the present invention, system still keeps closure; Reduced the probability that system is polluted, therefore can continue to keep Continuous Cultivation, the cell homogeneity is improved; Little algae photosynthetic efficiency and cell culture efficiency obviously strengthen, and cultured cells density further increases by 50%.
Claims (9)
1. the method for cleaning of a pipeline bioreactor inside pipe wall; It is characterized in that: between the inlet ductwork and export pipeline of pipeline bioreactor; The circulating line bypass is set; In the circulating line bypass, gauge tap is set, in the circulatory system of circulating line bypass and pipeline bioreactor formation, sets up at least one magnetic spheres;
Magnetic spheres is put into the pipeline bioreactor; Nutrient solution begins to flow under dynamic action; Magnetic spheres gets in the pipeline of bioreactor at random and carries out orientation and move with nutrient solution; Perhaps receive pipeline outer control system's manual adjustment and slide; Magnetic spheres constantly produces slight impact and friction with tube wall in the circulation moving process in the Photoreactor pipeline, thereby the frustule material that will paste on the transparent pipeline inwall rubs in the liquid phase of culturing microalgae, and the pipeline bioreactor is effectively cleared up and kept its light transmission;
Magnetic spheres has the inside and outside double-layer structure that is provided with; Inner can produce magnetic or through inducing the material of generation magnetic for rigidity; Outside plastic material for wrapping up magnet, avoiding its crash response organ pipe wall, have shrinkage, the overall proportion of magnetic spheres equates with the proportion of micro algae culturing liquid or is bigger.
2. according to the method for cleaning of the described pipeline bioreactor of claim 1 inside pipe wall; It is characterized in that: the magnetic spheres occluding device is set on the export pipeline of pipeline bioreactor, and the magnetic spheres occluding device is positioned at the circulatory system outside of circulating line bypass and pipeline bioreactor formation;
Magnetic spheres arrives the occluding device position after accomplishing the cleaning of pipeline bioreactor, and magnetic spheres is blocked, and nutrient solution gets into the recovery of algae liquid, Gas Exchange and temperature control tower smoothly through this magnetic spheres occluding device; The magnetic spheres of being obstructed gets into the circulating line bypass under the effect of gravity and magnetic attracting force, reclaim, be deposited in the upper side position of gauge tap.
3. according to the method for cleaning of the described pipeline bioreactor of claim 2 inside pipe wall, it is characterized in that: the magnetic spheres occluding device adopts the network structure that can stop magnetic spheres to be passed through.
4. according to the method for cleaning of claim 1 or 2 described pipeline bioreactor inside pipe walls; It is characterized in that: the size of each magnetic spheres equates with the pipeline internal diameter of bioreactor basically; The pipeline of bioreactor and magnetic spheres adopt matched in clearance, and magnetic spheres is just moved at the pipeline of bioreactor.
5. according to the method for cleaning of the described pipeline bioreactor of claim 1 inside pipe wall, it is characterized in that: the mesh aperture on the occluding device is less than whole magnetic spheres diameter, and the magnet diameter middle than magnetic spheres of the mesh aperture on the occluding device is little.
6. according to the method for cleaning of the described pipeline bioreactor of claim 1 inside pipe wall, it is characterized in that:
Attract control device in pipeline bioreactor peripheral hardware magnetic spheres, magnetic spheres attracts control device guiding magnetic spheres to get into the circulation bypass or rest on the somewhere.
7. according to the method for cleaning of the described pipeline bioreactor of claim 1 inside pipe wall; It is characterized in that: an end of circulating line bypass is connected on the pipeline between pipeline bioreactor light accepting part branch and the recovery of algae liquid, Gas Exchange and the temperature control tower, and magnetic spheres attracts control device to be arranged at an end of said circulating line bypass; The other end of circulating line bypass is connected between pipeline bioreactor light accepting part branch and the kinetic pump, and the switching of this circulating line bypass is controlled by the ball-type gauge tap.
8. according to the method for cleaning of the described pipeline bioreactor of claim 1 inside pipe wall; It is characterized in that: kinetic pump, switch I, switch I I are set on the inlet ductwork of pipeline bioreactor; Switch I, kinetic pump are positioned at a side of circulating line bypass in order, and switch I I is positioned at the opposite side of circulating line bypass;
Close kinetic pump, open gauge tap, magnetic spheres is fallen the downside of gauge tap; Closing control switch then; Restart kinetic pump; Magnetic spheres gets in the pipeline bioreactor again, produces friction with tube wall, and the frustule material that pastes on the transparent pipeline inwall is rubbed in the liquid phase of culturing microalgae; Circulation is so repeatedly effectively cleared up the attached cell on the pipeline bioreactor and is kept its light transmission.
9. according to the method for cleaning of the described pipeline bioreactor of claim 1 inside pipe wall; It is characterized in that: little algae comes from freshwater microalgae, salt water or the briny environment, comprises flat algae, chlorella, chrysophyceae, grid algae, haematococcus, purple ball algae, snow algae or diatom.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010105009269A CN102000679B (en) | 2010-09-30 | 2010-09-30 | Method for cleaning inner wall of pipeline type photobioreactor pipe |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010105009269A CN102000679B (en) | 2010-09-30 | 2010-09-30 | Method for cleaning inner wall of pipeline type photobioreactor pipe |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102000679A CN102000679A (en) | 2011-04-06 |
| CN102000679B true CN102000679B (en) | 2012-07-25 |
Family
ID=43808528
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010105009269A Expired - Fee Related CN102000679B (en) | 2010-09-30 | 2010-09-30 | Method for cleaning inner wall of pipeline type photobioreactor pipe |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102000679B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106906116A (en) * | 2015-12-22 | 2017-06-30 | 国家开发投资公司 | Pipe arranges the cleaning device and bioreactor of formula bioreactor |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106906118B (en) * | 2015-12-22 | 2019-07-19 | 国投生物科技投资有限公司 | The cleaning device and bioreactor of bioreactor |
| CN108611262A (en) * | 2018-04-17 | 2018-10-02 | 曾坚 | Self-cleaning inner tubal wall duct type bioreactor |
| CN108893404A (en) * | 2018-07-19 | 2018-11-27 | 佛山市宝粤美科技有限公司 | A kind of sampler and its application method based on pipeline cultivation algae |
| CN110423681A (en) * | 2019-09-17 | 2019-11-08 | 肖卫武 | A kind of photosynthetic microorganism culture production system |
| CN113403197B (en) * | 2021-05-14 | 2024-04-09 | 江苏大学 | Photo-biological reaction system and method for culturing microalgae by utilizing magnetic field coupling |
| CN113941572B (en) * | 2021-09-27 | 2022-10-25 | 宁波科尼管洁净科技有限公司 | Steel pipe circulating cleaning method and equipment |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4234993A (en) * | 1979-05-30 | 1980-11-25 | Kintner Edwin K | Condenser cleaning system using sponge balls |
| EP0026261A1 (en) * | 1979-08-14 | 1981-04-08 | Werner Borchert | Device for separating cleaning bodies from a fluid flowing out of a shell-and-tube heat exchanger |
| CN2580386Y (en) * | 2002-09-28 | 2003-10-15 | 倪永刚 | On-line automatic cleaning device for condensator heat exchange tube inner wall |
| CN1778482A (en) * | 2004-11-25 | 2006-05-31 | 杜家财 | Cleaner of electric water heater |
| CN2906528Y (en) * | 2006-04-27 | 2007-05-30 | 肖继恺 | Solar heat collecting pipe cleaning device |
| TW201027026A (en) * | 2009-01-13 | 2010-07-16 | zheng-fa Zhou | Heat-exchange cleaning system |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2002210428A (en) * | 2001-01-19 | 2002-07-30 | Chubu Electric Power Co Inc | Ball for cleaning inside of pipe, and cleaning pig for inspecting passage of pipe |
| JP3362340B1 (en) * | 2002-04-15 | 2003-01-07 | 絹児 桑島 | In-pipe cleaning tool and method of manufacturing the same |
-
2010
- 2010-09-30 CN CN2010105009269A patent/CN102000679B/en not_active Expired - Fee Related
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4234993A (en) * | 1979-05-30 | 1980-11-25 | Kintner Edwin K | Condenser cleaning system using sponge balls |
| EP0026261A1 (en) * | 1979-08-14 | 1981-04-08 | Werner Borchert | Device for separating cleaning bodies from a fluid flowing out of a shell-and-tube heat exchanger |
| CN2580386Y (en) * | 2002-09-28 | 2003-10-15 | 倪永刚 | On-line automatic cleaning device for condensator heat exchange tube inner wall |
| CN1778482A (en) * | 2004-11-25 | 2006-05-31 | 杜家财 | Cleaner of electric water heater |
| CN2906528Y (en) * | 2006-04-27 | 2007-05-30 | 肖继恺 | Solar heat collecting pipe cleaning device |
| TW201027026A (en) * | 2009-01-13 | 2010-07-16 | zheng-fa Zhou | Heat-exchange cleaning system |
Non-Patent Citations (2)
| Title |
|---|
| JP特开2002-210428A 2002.07.30 |
| JP特开2003-305423A 2003.10.28 |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106906116A (en) * | 2015-12-22 | 2017-06-30 | 国家开发投资公司 | Pipe arranges the cleaning device and bioreactor of formula bioreactor |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102000679A (en) | 2011-04-06 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102000679B (en) | Method for cleaning inner wall of pipeline type photobioreactor pipe | |
| Xu et al. | Development of integrated culture systems and harvesting methods for improved algal biomass productivity and wastewater resource recovery–a review | |
| KR101148194B1 (en) | Photobioreactor with Transparent Film | |
| CN102134553B (en) | Tubular photobioreactor and system and method for culturing microalgae cells | |
| CN102206570B (en) | Apparatus and cultivating method for scaled cultivation of microalgae | |
| Huang et al. | Improvement on light penetrability and microalgae biomass production by periodically pre-harvesting Chlorella vulgaris cells with culture medium recycling | |
| CN102586075B (en) | Self-cleaning tubular photobioreactor | |
| CN1213139C (en) | Photosynthesis bioreactor system for industrial production of micro algae | |
| CN102382754B (en) | Optical fiber photobioreactor for making full use of 'flash effect' of microalgae | |
| CN102583767B (en) | System for treating sewage and producing biological oil by using microalgae and method | |
| CN102329720B (en) | Photobioreactor capable of realizing high-efficiency carbon dioxide immobilization | |
| CN107189930A (en) | Indoor microalgae culture system and its cultural method | |
| CN105002094A (en) | Method for semi-continuously culturing marine microalgae through solar greenhouse | |
| CN101906380B (en) | Seal type pipeline culture device of bait microalgae and microalgae pipeline culture method | |
| Mahmoud et al. | Closed photobioreactor for microalgae biomass production under indoor growth conditions | |
| CN107641594B (en) | Differential pressure type open pipeline bioreactor for microalgae culture | |
| CN201729830U (en) | Sealed pipeline culturing device for bait microalgae | |
| CN203462044U (en) | Cylindrical photobioreactor for bait microalgae cultivation | |
| CN106635768B (en) | Biological microalgae photosynthetic reactor and its application method | |
| CN102296025B (en) | Photobioreactor capable of realizing internal and external circulation of alga liquid | |
| CN201381319Y (en) | Optical bioreactor for nursing microalgae | |
| CN102559478A (en) | Controllable slope type microalgae cultivation system and microalgae cultivation method thereof | |
| CN201420080Y (en) | Sealed microalgae industry photosynthesis cultivating device | |
| CN102373151A (en) | Photobioreactor and photobiology culture system | |
| CN102373150A (en) | Photobioreactor and photobiology culture system |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20120725 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |