CN108893404A - A kind of sampler and its application method based on pipeline cultivation algae - Google Patents
A kind of sampler and its application method based on pipeline cultivation algae Download PDFInfo
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- CN108893404A CN108893404A CN201810797956.7A CN201810797956A CN108893404A CN 108893404 A CN108893404 A CN 108893404A CN 201810797956 A CN201810797956 A CN 201810797956A CN 108893404 A CN108893404 A CN 108893404A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/22—Transparent or translucent parts
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/06—Tubular
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
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- General Health & Medical Sciences (AREA)
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Abstract
The invention discloses a kind of sampler and its application method based on pipeline cultivation algae, the first sampling box and the second sampling box including pipeline body, in pipeline body, the first annulus outside pipeline body and the second annulus;The pipeline body is transparent glass tube, first sampling box is corresponding with first annulus position, second sampling box is corresponding with second annulus position, first sampling box can follow first annulus to be moved along the pipeline body length direction, second sampling box can follow second annulus to be moved along the pipeline body length direction, first sampling box is matched with second sampling box, and first annulus is matched with second annulus.The present invention can accurately sample the algae in pipeline, to obtain the growth data of algae in pipeline by experiment.
Description
Technical field
The invention belongs to pipeline cultural technique field more particularly to it is a kind of based on pipeline cultivation algae sampler and its make
Use method.
Background technique
Algae is also known as thallophyte, is that one kind has chlorophyll, can be carried out photosynthesis, battalion's photosynthetic autotrophs life
It is living without root, stem and leaf differentiation, without vascular bundle, without the thallose of embryo, be broadly divided into planktonic algae, float algae, benthic algae
Three classes.
The height of the algae as present in nature mixes and disperses, and there is no a large amount of single algae directly to receive
Collection, so need to carry out the culture of algae using the mode that pipeline cultivates.
During pipeline cultivates algae, when needing the algae to some position in pipeline to be sampled investigation, mesh
Algae at pipe ends position can only be sampled as previous, can not to being sampled operation at pipeline center position, therefore
And it can not precisely understand the growing state of the algae at pipeline center position.
Summary of the invention
The present invention overcomes the deficiencies in the prior art, provide a kind of sampler and its user that algae is cultivated based on pipeline
Method can accurately sample the algae in pipeline, to obtain the growth data of algae in pipeline by experiment.
In order to achieve the above objectives, the technical solution adopted by the present invention is:A kind of sampler based on pipeline cultivation algae, packet
Include pipeline body, the first sampling box in pipeline body and the second sampling box, the first annulus outside pipeline body with
Second annulus;The pipeline body is transparent glass tube, and first sampling box is corresponding with first annulus position, described
Second sampling box is corresponding with second annulus position, and first sampling box can follow first annulus along the pipe
Road body length direction is moved, and second sampling box can follow second annulus along the pipeline body length side
To being moved, first sampling box is matched with second sampling box, first annulus and the second annulus phase
Cooperation.
In a preferred embodiment of the present invention, first sampling box is stretched with the ipsilateral metal that is provided with of second sampling box
Contracting bar, first annulus outer surface are provided with the first electromagnet, and second annulus outer surface is provided with the second electromagnet.
In a preferred embodiment of the present invention, the Telescopic metal bar is iron, cobalt, nickel metalwork.
In a preferred embodiment of the present invention, first sampling box, the second sampling box, the first annulus, the second annulus are equal
For plastic part.
In a preferred embodiment of the present invention, first electromagnet is electrically connected with external power supply with second electromagnet
It connects.
In a preferred embodiment of the present invention, first sampling box one end is provided with sampling fixture block, second sampling
Box one end adjacent with first sampling box is provided with the sampling groove matched with the sampling fixture block.
In a preferred embodiment of the present invention, first annulus side is provided with casting lug thereon, and second annulus is close
First annulus side is provided with the locating slot matched with the casting lug thereon.
In a preferred embodiment of the present invention, include the following steps:
(1) it is powered, the first electromagnet, the equal band of the second electromagnet is made to be magnetic;
(2) the first sampling box+Telescopic metal bar, the second sampling box+Telescopic metal bar are put from pipeline body both ends respectively
Enter in pipeline body, the Telescopic metal bar at both ends is respectively by the first electromagnet, the magneticaction of the second electromagnet, in pipeline sheet
It is fixed in vivo;
(3) mobile first annulus, the second annulus make the first annulus, the second annulus reach sample position, the first sampling box with
Second sampling box, which matches, to be sampled, and after the completion of sampling, the first sampling box and the second sampling box shape are integral, the first annulus
It is integral with the second circular ring shape;
(4) annulus after formation entirety is removed from pipeline body one end, is formed simultaneously whole sampling box and follows annulus
It is moved, sample is taken out in synchronization.
In a preferred embodiment of the present invention, the Telescopic metal bar and first sampling box, second sampling box
It is respectively fixedly connected with.
In a preferred embodiment of the present invention, first sampling box and second sampling box pass through a buckle structure shape
At sampling box entirety, first annulus and second annulus are whole by another buckle structure formation annulus.
The invention solves the defect existing in the background technology, and the present invention has following beneficial effect:
(1) the first sampling box is driven to be moved in pipeline body by a Telescopic metal bar using the first electromagnet,
Second electromagnet drives the second sampling box to be moved in pipeline body by another Telescopic metal bar, guarantees the first sampling
Box, the second sampling box can smoothly reach sample position, and then smoothly complete sampling operation, ensure that the accuracy of sampling;
(2) first sampling boxes are connect with the second sampling box using buckle structure, guarantee smoothly to clamp sample;
The use of (3) first annulus and the second annulus can be led to the first sampling box by the first electromagnet, the second sampling box
It crosses the second electromagnet to send to sampling point, realizes the accurate sampling to algae;
(4) use of Telescopic metal bar, guarantee the first sampling box, the second sampling box can smoothly by the first electromagnet,
The magneticaction of second electromagnet, while the position of sampling box can be changed by the way of flexible, and then change sample position.
Detailed description of the invention
Present invention will be further explained below with reference to the attached drawings and examples;
Fig. 1 is the overall structure diagram of the preferred embodiment of the present invention;
Fig. 2 is the portion A enlarged drawing in Fig. 1;
Fig. 3 is the right view of the preferred embodiment of the present invention;
Fig. 4 is the partial structural diagram of the preferred embodiment of the present invention;
In figure:1, pipeline body;2, Telescopic metal bar;3, the first sampling box;31, fixture block is sampled;4, the second sampling box;
41, sampling groove;5, the first annulus;51, casting lug thereon;6, the second annulus;61, locating slot;7, the first electromagnet;8, the second electromagnetism
Iron.
Specific embodiment
Presently in connection with drawings and examples, the present invention is described in further detail, these attached drawings are simplified signal
Figure, the basic structure of the invention will be illustrated schematically only, therefore it only shows the composition relevant to the invention.
As shown in Figure 1, Figure 2, Figure 3 and Figure 4, a kind of sampler based on pipeline cultivation algae, including pipeline body 1, position
In the first sampling box 3 and the second sampling box 4, the first annulus 5 and the second annulus outside pipeline body 1 in pipeline body 1
6;Pipeline body 1 is transparent glass tube, and the first sampling box 3 is corresponding with 5 position of the first annulus, the second sampling box 4 and the second circle
6 position of ring is corresponding, and the first sampling box 3 can follow the first annulus 5 to be moved along 1 length direction of pipeline body, and second takes
Sample box 4 can follow the second annulus 6 to be moved along 1 length direction of pipeline body, the first sampling box 3 and 4 phase of the second sampling box
Cooperation, the first annulus 5 are matched with the second annulus 6, and the present invention uses the first sampling box 3 and the second sampling box 4 to carry as sampling
Body is sampled operation to the algae in pipeline body 1, and the first sampling box 3 and the second sampling box 4 are respectively from 1 liang of pipeline body
Hair is brought out, after reaching sample position, two sampling boxes are combined, and clamp sample, then will form whole sampling box out of pipeline body 1
It takes out, completes sampling operation.
Further, the first sampling box 3 and the second sampling box 4 is ipsilateral is provided with Telescopic metal bar 2,5 appearance of the first annulus
Face is provided with the first electromagnet 7, and 6 outer surface of the second annulus is provided with the second electromagnet 8, passes through a gold medal using the first electromagnet 7
Belonging to telescopic rod 2 drives the first sampling box 3 to be moved in pipeline body 1, and the second electromagnet 8 passes through another Telescopic metal bar 2
It drives the second sampling box 4 to be moved in pipeline body 1, guarantees that the first sampling box 3, the second sampling box 4 can be reached smoothly
Sample position, and then smoothly complete sampling operation, ensure that the accuracy of sampling, while the first annulus 5 and the second annulus 6 make
With the first sampling box 3 can be sent by the second electromagnet 8 to sampling point by the first electromagnet 7, the second sampling box 4, realized
Accurate sampling to algae, the use of Telescopic metal bar 2 guarantee that the first sampling box 3, the second sampling box 4 can be smoothly by the
The magneticaction of one electromagnet 7, the second electromagnet 8, while the position of sampling box, Jin Ergai can be changed by the way of flexible
Become sample position.
Specifically, Telescopic metal bar 2 is iron, cobalt, nickel metalwork are guaranteed using iron-cobalt-nickel metalwork by electromagnetism
The magneticaction of iron, to be moved.
Specifically, the first sampling box 3, the second sampling box 4, the first annulus 5, the second annulus 6 are plastic part, using plastics
Part avoids generating interference with electromagnet.
Further, the first electromagnet 7 is electrically connected with external power supply with the second electromagnet 8, so as to pass through control
The on-off of power supply, so control electromagnet it is magnetic whether there is or not.
Specifically, 3 one end of the first sampling box is provided with sampling fixture block 31, the second sampling box 4 is adjacent with the first sampling box 3
One end is provided with the sampling groove 41 matched with sampling fixture block 31, is matched, is guaranteed with sampling groove 41 using sampling fixture block 31
Sampling operation is enough gone on smoothly, after clamping, algae is torn by force, is taken out.
Specifically, 5 side of the first annulus is provided with casting lug thereon 51, the second annulus 6 is provided with close to 5 side of the first annulus
The locating slot 61 matched with casting lug thereon 51, casting lug thereon 51 are matched with locating slot 61, make 6 shape of the first annulus 5 and the second annulus
It is integral, after the completion of sampling operation, moved to 1 both ends a direction of pipeline body.
The present invention in use, includes the following steps:
(1) it is powered, the first electromagnet 7, the equal band of the second electromagnet 8 is made to be magnetic;
(2) by the first sampling box 3+ Telescopic metal bar 2, the second sampling box 4+ Telescopic metal bar 2 respectively from 1 liang of pipeline body
End is put into pipeline body 1, the Telescopic metal bar 2 at both ends respectively by the first electromagnet 7, the magneticaction of the second electromagnet 8,
It is fixed in pipeline body 1;
(3) mobile first annulus 5, the second annulus 6 make the first annulus 5, the second annulus 6 reach sample position, the first sampling
Box 3 is matched with the second sampling box 4 and is sampled, and after the completion of sampling, the first sampling box 3 and 4 shape of the second sampling box are integral,
First annulus 5 and 6 shape of the second annulus are integral;
(4) annulus after formation entirety is removed from 1 one end of pipeline body, is formed simultaneously whole sampling box and follows annulus
It is moved, sample is taken out in synchronization.
Wherein:The first step, after energization, the first electromagnet 7 and the second electromagnet 8 have stable magnetism, can be to gold
Belong to telescopic rod 2 and generates magnetic force;Two Telescopic metal bars 2 are respectively put into 1 both ends of pipeline body by second step, the first electromagnet 7,
Second electromagnet 8 and corresponding Telescopic metal bar 2 generate magnetic force, make the position of the first sampling box 3 and the second sampling box 4 same
On straight line;Third step, the first annulus 5, the second annulus 6 is mobile, when the two reaches sample position, the first annulus 5 and second
6 shape of annulus is integral, and the first sampling box 3 in pipeline body 1,4 shape of the second sampling box are integral;4th step is formed whole
The annulus of body is removed from 1 one end of pipeline body, and at the same time, forming whole sampling box will profile-followed integral annulus progress
Movement, sample, which synchronizes, to be removed.
Specifically, Telescopic metal bar 2 is respectively fixedly connected with the first sampling box 3, the second sampling box 4, using being fixedly connected,
Guaranteeing that metal telescopic rod 2 can be stablized during the motion drives sampling box to be moved.
Further, the first sampling box 3 and the second sampling box 4 are whole by buckle structure formation sampling box, using card
Buckle structure can preferably be sampled operation, and the first annulus 5 is whole by another buckle structure formation annulus with the second annulus 6
Body.
To sum up, the present invention drives the first sampling box 3 in pipeline using the first electromagnet 7 by a Telescopic metal bar 2
Moved in ontology 1, the second electromagnet 8 by another Telescopic metal bar 2 drive the second sampling box 4 in pipeline body 1 into
Row movement guarantees that the first sampling box 3, the second sampling box 4 can smoothly reach sample position, after reaching sample position, two samplings
Box combines, and clamps sample, and then smoothly complete sampling operation, ensure that the accuracy of sampling, while the first annulus 5 and the second circle
The use of ring 6 can be sent the first sampling box 3 by the second electromagnet 8 to sampling portion by the first electromagnet 7, the second sampling box 4
The accurate sampling to algae is realized in position.
Based on the above description of the preferred embodiments of the present invention, through the above description, related personnel completely can be with
Without departing from the scope of the technological thought of the present invention', various changes and amendments are carried out.The technical scope of this invention
It is not limited to the contents of the specification, it is necessary to determine the technical scope according to the scope of the claims.
Claims (10)
1. a kind of sampler based on pipeline cultivation algae, it is characterised in that:Including pipeline body, in pipeline body
One sampling box and the second sampling box, the first annulus and the second annulus outside pipeline body;The pipeline body is transparent glass
Glass pipe, first sampling box is corresponding with first annulus position, second sampling box and second annulus position
Corresponding, first sampling box can follow first annulus to be moved along the pipeline body length direction, described
Second sampling box can follow second annulus to be moved along the pipeline body length direction, first sampling box with
Second sampling box matches, and first annulus is matched with second annulus.
2. a kind of sampler based on pipeline cultivation algae according to claim 1, it is characterised in that:First sampling
Box with second sampling box is ipsilateral is provided with Telescopic metal bar, first annulus outer surface is provided with the first electromagnet, institute
It states the second annulus outer surface and is provided with the second electromagnet.
3. a kind of sampler based on pipeline cultivation algae according to claim 2, it is characterised in that:The Telescopic metal
Bar is iron, cobalt, nickel metalwork.
4. a kind of sampler based on pipeline cultivation algae according to claim 2, it is characterised in that:First sampling
Box, the second sampling box, the first annulus, the second annulus are plastic part.
5. a kind of sampler based on pipeline cultivation algae according to claim 2, it is characterised in that:First electromagnetism
Iron is electrically connected with external power supply with second electromagnet.
6. a kind of sampler based on pipeline cultivation algae according to claim 1, it is characterised in that:First sampling
Box one end is provided with sampling fixture block, and second sampling box one end adjacent with first sampling box is provided with to press from both sides with the sampling
The sampling groove that block matches.
7. a kind of sampler based on pipeline cultivation algae according to claim 1, it is characterised in that:First annulus
Side is provided with casting lug thereon, and second annulus is provided with close to first annulus side to be determined with what the casting lug thereon matched
Position slot.
8. a kind of application method of sampler based on pipeline cultivation algae according to claim 1, it is characterised in that:Packet
Include following steps:
(1) it is powered, the first electromagnet, the equal band of the second electromagnet is made to be magnetic;
(2) the first sampling box+Telescopic metal bar, the second sampling box+Telescopic metal bar are put into pipe from pipeline body both ends respectively
In road ontology, the Telescopic metal bar at both ends is respectively by the first electromagnet, the magneticaction of the second electromagnet, in pipeline body
It is fixed;
(3) mobile first annulus, the second annulus make the first annulus, the second annulus reach sample position, the first sampling box and second
Sampling box, which matches, to be sampled, and after the completion of sampling, the first sampling box and the second sampling box shape are integral, the first annulus and
Two circular ring shapes are integral;
(4) annulus after formation entirety is removed from pipeline body one end, is formed simultaneously whole sampling box and annulus is followed to carry out
Sample is taken out in movement, synchronization.
9. a kind of application method of sampler based on pipeline cultivation algae according to claim 8, it is characterised in that:Institute
Telescopic metal bar is stated to be respectively fixedly connected with first sampling box, second sampling box.
10. a kind of application method of sampler based on pipeline cultivation algae according to claim 8, it is characterised in that:
It is whole that first sampling box and second sampling box by a buckle structure form sampling box, first annulus with it is described
Second annulus forms annulus entirety by another buckle structure.
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CN201810797956.7A CN108893404A (en) | 2018-07-19 | 2018-07-19 | A kind of sampler and its application method based on pipeline cultivation algae |
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Cited By (1)
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CN109628286A (en) * | 2019-01-15 | 2019-04-16 | 泰普生物科学(中国)有限公司 | A kind of transport culture apparatus |
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