CN213652516U

CN213652516U - Gas dispersion device for stem cell culture tank

Info

Publication number: CN213652516U
Application number: CN202020866814.4U
Authority: CN
Inventors: 华子昂
Original assignee: Individual
Current assignee: Individual
Priority date: 2020-05-18
Filing date: 2020-05-18
Publication date: 2021-07-09
Anticipated expiration: 2030-05-18

Abstract

The utility model provides a stem cell is for jar gas dispersion devices encloses into ventilated membrane bag chamber with the ventilated membrane, regulates and control stem cell through intake pipe and blast pipe and cultivates the demand to gas.

Description

Gas dispersion device for stem cell culture tank

Technical Field

The utility model belongs to the technical field of stem cell culture and specifically relates to a stem cell is gas dispersion devices for culture tank.

Background

The stem cells have a variety of types and are widely applied in the fields of disease treatment, medical and beauty treatment, anti-aging, drug screening and the like. Stem cell culture has become a bottleneck for the development of the whole stem cell industry chain, and stem cell culture devices have become hot spots for the development of the stem cell culture field.

The current stem cell culture device is mainly from the microbial fermentation industry, and a culture tank is directly or simply adjusted for stem cell production. Because stem cells are not protected by cell walls like bacteria, the stirring paddle in the bacterial culture tank can cause great shearing force damage to the stem cells in culture, and can cause death of the stem cells or induce stem cell variation. The mild stirring mode can not effectively ensure the gas requirement in the stem cell culture process, the increase of the gas flow can solve the requirement of stem cell growth to a certain extent, but also increases the mechanical damage to the stem cells.

Disclosure of Invention

The utility model provides a gas dispersing device for a stem cell culture tank, which essentially utilizes a gas permeable membrane to form finer bubbles to replace the traditional method of directly releasing gas through a small hole, thereby avoiding the stem cells from being damaged by the impact of air flow caused by the gas release through the small hole; meanwhile, the contact area between the stem cells and the bubbles is enlarged, the gas exchange rate is accelerated, and the demand of the stem cells on gas metabolism is effectively guaranteed.

The utility model discloses a concrete technical scheme is: the bottom of the stem cell culture tank is provided with the breathable membrane bag, gas in the breathable membrane bag escapes from the breathable membrane bag in a micro bubble form under the action of pressure, so that the impact of large bubbles on stem cells is avoided, meanwhile, the total surface area of the bubbles can be increased by utilizing the micro bubbles, the full contact with the stem cells is strengthened, and the gas exchange is completed. The air inlet pipe and the air outlet pipe are arranged in the breathable membrane bag, so that the gas composition in the breathable membrane bag can be quickly adjusted, and the requirement of stem cell growth is met. When the stem cells need to be supplied with gas, the gas pressure in the gas inlet pipe is increased, so that the gas can enter the stem cell culture solution, and the growth requirements of the stem cells are met; when the stem cells need to condition the gas composition, the gas inlet pipe and the gas outlet pipe are opened simultaneously, and the gas composition is rapidly adjusted.

The beneficial effects of the utility model are that can carry out gas transportation fast according to the needs that stem cell grows, effectively satisfy stem cell growth demand.

Drawings

FIG. 1 is a schematic view (sectional view) showing the overall structure of a gas dispersion apparatus for a stem cell culture tank

FIG. 2 is a schematic view of an assembly effect of the embodiment

Breathable film bag 1, breathable film bag chamber 2, intake pipe 3, blast pipe 4 merges air duct 5, and sealed cutting ferrule 6, breathable film inner support layer 7, ventilated film 8, ventilated film outer support layer 9, cultivates jar cylinder body 10, cultivates jar top cap 11, cultivates jar bottom 12, sealed axle sleeve 13, take the (mixing) shaft 14 of paddle, sensor group 15.

Detailed Description

The culture tank body 10 is placed on an operation table, and the sealed assembly of the culture tank bottom 12 and the culture tank body 10 is completed.

A sealing shaft sleeve 13, a stirring shaft 14 with blades and a sensor group 15 are arranged on the culture tank top cover 11, the combined air duct 5 passes through the stem cell culture tank top cover 11 and is simply fixed by a sealing cutting sleeve 6, and the pre-assembly of the culture tank top cover 11 is completed.

Placing the preassembled culture top cover 11 on the culture tank body 10 to complete the sealing of the stem cell culture tank; adjusting the relative position of the sealing cutting sleeve and the combined air duct 5 to place the air permeable membrane bag 1 at the bottom of the stem cell culture tank.

The exhaust pipe 4 is closed, gas is guided into the gas-permeable membrane bag cavity 2 through the gas inlet pipe 3, the gas passes through the gas-permeable membrane inner supporting layer 7, the gas-permeable membrane 8 and the gas-permeable membrane outer supporting layer 9 under the action of pressure to form micro bubbles, the micro bubbles enter the stem cell culture solution, the gas exchange between the micro bubbles and stem cells can be rapidly completed, and the growth requirement of the stem cells is met.

The pressure of the exhaust pipe 4 and the air inlet pipe 3 is adjusted, the gas composition adjustment in the gas permeable membrane bag cavity 2 can be rapidly completed, the gas with specific composition is ensured to rapidly enter the stem cell culture solution under the action of pressure, and the gas exchange is carried out by forming a large amount of micro bubbles and stem cells, so that the requirement of the stem cells on the gas at a specific growth stage is met.

The present embodiments are intended to embrace all such alternatives, modifications and variances which fall within the broad scope of the appended claims. Therefore, any omission, modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included within the protection scope of the present invention.

Claims

1. A gas dispersion device for a stem cell culture tank is characterized by comprising a breathable membrane bag, a breathable membrane bag cavity, a gas inlet pipe, a gas outlet pipe and a sealing clamping sleeve; the breathable film inner supporting layer and the breathable film outer supporting layer are coated with the breathable film to form a breathable film bag, a breathable film bag cavity is enclosed, the air inlet pipe and the air outlet pipe form a combined conduit side by side, and the combined conduit is fixed on the stem cell culture tank through an external sealing clamping sleeve.

2. The gas dispersion device for the stem cell culture tank as claimed in claim 1, wherein the gas permeable membrane sac is defined by a gas permeable membrane, an inner gas permeable membrane supporting layer and an outer gas permeable membrane supporting layer, and the inner cavity of the gas permeable membrane sac is a gas permeable membrane sac cavity.

3. The gas dispersion device for a stem cell culture tank as claimed in claim 1, wherein the gas permeable membrane bag is connected to the outside of the stem cell culture tank through a gas inlet pipe and a gas outlet pipe.

4. The gas dispersion device for a stem cell culture tank as claimed in claim 1, wherein the gas inlet pipe and the gas outlet pipe are arranged side by side to form a combined conduit which penetrates through the gas permeable membrane sac and is fixed on the stem cell culture tank through the sealing sleeve.