CN205710773U - High efficiency cell culture bag - Google Patents
High efficiency cell culture bag Download PDFInfo
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- CN205710773U CN205710773U CN201620302744.3U CN201620302744U CN205710773U CN 205710773 U CN205710773 U CN 205710773U CN 201620302744 U CN201620302744 U CN 201620302744U CN 205710773 U CN205710773 U CN 205710773U
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- bag
- cell
- cell culture
- high efficiency
- basement membrane
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Abstract
The utility model discloses a kind of high efficiency cell culture bag, including basement membrane, described basement membrane Butt sealing composition bag, in described basement membrane, overlay film is set, it is protruding that the inner surface of described overlay film arranges several, described bag top is provided with one end of feed tube and quality inspection pipe, described feed tube and quality inspection pipe and extraneous contact and is connected to screw-cap.The beneficial effects of the utility model are: cell bags seals, can disposably add culture fluid and medium exchange needed for cultivation cell, intermediary operation need not be carried out, reduce pollution risk, enormously simplify cell culture process flow process simultaneously, saved cell incubation time, inside overlay film, be additionally provided with projection, increase cell culturing surfaces to amass, cell is cultivated there is good supporting role.
Description
Technical field
This utility model relates generally to cell Industrialized processing technique field, particularly relates to a kind of high efficiency cell training
Support bag.
Background technology
Tumor vaccine cells treatment is a kind of Celluar and Molecular Biology means that use, enhancing patient's machine
The immune system of body, thus reach the Therapeutic Method suppressing tumor growth even to be eradicated.Substantial amounts of basis
Being proved tumor biotherapy for tumor patient with Clinical Evidence is one safely and effectively means, energy
Enough improve survival of patients treatment, extend life cycle, some patient even can be reached the curative effect cured.PD1 and
PDL1 antibody and mosaic antigen receptor identification T cell howling success in terms for the treatment of oncotherapy show
Immunization therapy advantage in terms of oncotherapy, that immunization therapy certainly will become oncotherapy one is indispensable
Means.
Cell of exempting from service is cultivated as in cell gene therapy important and link for core, to cell therapy
Effect plays decisive role, uses existing cell to cultivate instrument such as Tissue Culture Flask and culture bag etc., exists
Incubation time is long, and intermediary operation is many, pollutes probability high, and the shortcoming that cell yield is the most inadequate needs to have been resolved.
Utility model content
For solving deficiency of the prior art, this utility model provides a kind of high efficiency cell culture bag, cell bags
Seal, can disposably add culture fluid and medium exchange needed for cultivation cell, intermediary operation need not be carried out,
Reduce pollution risk, enormously simplify cell culture process flow process simultaneously, saved cell incubation time, separately
It is provided with projection inside external overlay film, increases cell culturing surfaces and amass, cell is cultivated there is good propping up
Support effect.
This utility model for achieving the above object, is achieved through the following technical solutions:
High efficiency cell culture bag, including basement membrane, described basement membrane Butt sealing composition bag, set in described basement membrane
Putting overlay film, the inner surface of described overlay film arranges that several are protruding, described bag top be provided with feed tube and
Quality inspection pipe, described feed tube and quality inspection pipe are connected to screw-cap with one end of extraneous contact.
Arranging barrier film in described bag, bag is divided into two parts by described barrier film, on described bag arrange with every
The opening that film matches, from feed tube to barrier film when injecting liquid in bag to bag away from a protruding sidesway
Dynamic until fitting with bag, when from opening to barrier film when injecting liquid in bag to the side of close the projection of bag
Mobile until fitting with bag.
Described basement membrane and overlay film are fep film.
Described projection is that point-like is protruding.
Described projection is hemispherical, half elliptic or triangle.
Described projection is raised strip.
The cross-sectional area of described projection is hemispherical, half elliptic or triangle.
Described projection is hemispherical, and the radius of a ball of projection is 0.005 μm.
The thickness of described bag is 5-10 μm.
On described feed tube, check valve is set.
Contrast and prior art, this utility model has the beneficial effects that:
1, this utility model bag includes overlay film and basement membrane, and overlay film has good breathability, it would be preferable to support thin
Born of the same parents' high-density growth, cell bags seals, and can disposably add culture fluid and medium exchange needed for cultivation cell,
Intermediary operation need not be carried out, reduce pollution risk, enormously simplify cell culture process flow process simultaneously, save
Production time, inside overlay film, additionally it is provided with projection, increases cell culturing surfaces and amass, cell is trained
Support and there is good supporting role.
2, arranging barrier film in bag described in the utility model, bag is divided into two parts by barrier film, can be according to carefully
The quantity that born of the same parents cultivate controls the input quantity of culture fluid, ensures that projection is all covered by culture fluid simultaneously.
3, basement membrane described in the utility model and overlay film are fep film, have good breathability and can support carefully
Born of the same parents' high-density growth.
4, this utility model projection is point-like or raised strip, increases cell culturing surfaces and amasss, and to carefully
Born of the same parents cultivate has good supporting role.
5, this utility model projection is hemispherical, half elliptic or triangle, increases cell further
Culture surface is amassed, and improves the supporting role to cell.
6, this utility model projection is hemispherical, and the radius of a ball of projection is 0.005 μm, makes cell hold very much
Easily adapt to protruding radian, be preferably attached on bag.
7, the thickness of this utility model bag is 5-10 μm, makes bag have good air permeable effect.
8, on feed tube, check valve is set, prevents the liquid in bag from entering feed tube, when preventing from injecting liquid,
Culture fluid container contact feed tube causes the pollution of culture fluid in container.
Accompanying drawing explanation
Accompanying drawing 1 is structural representation of the present utility model；
Accompanying drawing 2 is sectional view of the present utility model；
Accompanying drawing 3 is enlarged drawing at the I of accompanying drawing 2.
Label shown in accompanying drawing: 1, bag；11, basement membrane；12, overlay film；2, protruding；3, feed tube；
4, quality inspection pipe；41, check valve；5, screw-cap；6, barrier film；61, opening.
Detailed description of the invention
In conjunction with the drawings and specific embodiments, the utility model is described in further detail.Should be understood that these are implemented
Example is merely to illustrate this utility model rather than limits scope of the present utility model.In addition, it is to be understood that readding
After having read the content that this utility model is lectured, this utility model can be made various changing by those skilled in the art
Moving or amendment, these equivalent form of values fall within the application appended claims limited range equally.
High efficiency cell culture bag, including basement membrane 11, described basement membrane 11 Butt sealing composition bag 1, described base
Arranging overlay film 12 in film 11, overlay film 12 has good breathability, it would be preferable to support high-density cell growth,
Cell bags seals.The inner surface of described overlay film 12 is evenly equipped with several projections 2, and described bag 1 top sets
Being equipped with feed tube 3 and quality inspection pipe 4, described feed tube 3 and quality inspection pipe 4 are connected respectively with one end of extraneous contact
There is screw-cap 5, seal.By feed tube 3 can disposably add cultivation cell needed for culture fluid and
Medium exchange, need not carry out intermediary operation, reduces pollution risk, also greatly simplify cell simultaneously and cultivates work
Process flow, has saved the production time.Quality inspection pipe 4 can facilitate personnel monitoring's cell in incubation
The progress cultivated and effect, cultivate and from quality inspection pipe 4, culture fluid released bag 1, by cell culture bags after terminating
Interior culture fluid, by different pipeline turnover bags 1, prevents feed tube 3 from contacting it with culture fluid in bag 1
After contact with culture fluid container again, it is to avoid the culture fluid in culture fluid container is polluted.
In order to control the input quantity of culture fluid according to the quantity that cell is cultivated, ensure that culture fluid is by convex simultaneously
Playing 2 all to cover, arrange barrier film 6 in described bag 1, bag 1 is divided into two parts by described barrier film 6, institute
State the opening 61 that on bag 1, setting matches with barrier film 6, from feed tube 3 to when injecting liquid in bag 1
Barrier film 6 to bag 1 away from protruding 2 a side shifting until fitting with bag 1, when from opening 61 to bag
When injecting liquid in 1, barrier film 6 is to the close side shifting of protruding 2 of bag 1, until fitting with bag 1, when
Need the cell quantity cultivated can inject pure water in opening 61 time less, make barrier film 6 lean on to bag 1
A nearly side shifting of protruding 2 reduces the use of the volume reducing culture fluid of bag 1 another part, ensures simultaneously
Projection 2 is all covered by culture fluid, reduces the cost that cell is cultivated.
High-density cell growth, described basement membrane 11 He is supported in order to enable bag 1 to have good breathability
Overlay film 12 is fep film.
Amass to increase cell culturing surfaces, and cell cultivation is had good supporting role, described
Protruding 2 is point-like projection 2.
Increase cell culturing surfaces amass to increase further, and improve the supporting role to cell, institute
State protruding 2 hemispherical, half elliptic or trianglees, make projection 2 have under same volume bigger
Surface area, can guarantee that again the smooth surface of protruding 2 simultaneously, increases cell yield.
In order to make cell be easily adapted to the radian of protruding 2, preferably it is attached on bag 1, described protruding 2
Hemispherical, and the radius of a ball of protruding 2 is 0.005 μm.
It is 5-10 μm to make bag 1 have the thickness of bag 1 described in good air permeable effect.
During in order to prevent from injecting liquid, culture fluid container contact feed tube 3 causes the pollution of culture fluid in container,
On described feed tube 3, check valve 41 is set.
Embodiment:
Embodiment 1:
High efficiency cell culture bag, including basement membrane 11, described basement membrane 11 Butt sealing composition bag 1, described bag
The thickness of body 1 is 5-10 μm, arranges overlay film 12 in described basement membrane 11, and described basement membrane 11 and overlay film 12 are equal
For fep film, overlay film 12 has good breathability can support high-density cell growth, and cell bags seals.
The inner surface of described overlay film 12 is evenly equipped with several projections 2, and described protruding 2 is point-like projection 2, in half
Oval.Described bag 1 top is provided with feed tube 3 and quality inspection pipe 4, described feed tube 3 is arranged unidirectional
Valve 41, described feed tube 3 and quality inspection pipe 4 are connected to screw-cap 5 with one end of extraneous contact, described
Arranging barrier film 6 in bag 1, bag 1 is divided into two parts by described barrier film 6, on described bag 1 arrange with every
The opening 61 that film 6 matches, from feed tube 3 to barrier film 6 when injecting liquid in bag 1 to bag 1 away from
One side shifting of protruding 2 until fitting with bag 1, when from opening 61 to barrier film when injecting liquid in bag 1
6 to bag 1 near protruding 2 a side shifting until fitting with bag 1.Having the beneficial effects that of the present embodiment:
Arranging barrier film 6 in described bag 1, bag 1 is divided into two parts by barrier film 6, the number can cultivated according to cell
Amount controls the input quantity of culture fluid, ensures that projection 2 is all covered by culture fluid simultaneously；Basement membrane 11 and overlay film 12
It is fep film, there is good breathability and can support high-density cell growth；Protruding 2 is point-like or band
Shape is protruding, increases cell culturing surfaces and amasss, and cell cultivation is had good supporting role.
Embodiment 2:
High efficiency cell culture bag, including basement membrane 11, described basement membrane 11 Butt sealing composition bag 1, described base
Arranging overlay film 12 in film 11, overlay film 12 has good breathability can support high-density cell growth, carefully
Born of the same parents' bag seals.The inner surface of described overlay film 12 is evenly equipped with several projections 2, and described protruding 2 is that banding is convex
Rising, the described cross-sectional area of protruding 2 is hemispherical, half elliptic or triangle, and described bag 1 top sets
Being equipped with feed tube 3 and quality inspection pipe 4, described feed tube 3 and quality inspection pipe 4 are connected respectively with one end of extraneous contact
There is screw-cap 5, seal.Having the beneficial effects that of the present embodiment: raised strip is protruding relative to point-like
Projection 2 is linked to be banding increasing while surface area by 2, can with equipment by cell directly from raised strip
Scrape, facilitate the results of cell.
Embodiment 3:
High efficiency cell culture bag, including basement membrane 11, described basement membrane 11 Butt sealing composition bag 1, described base
Arranging overlay film 12 in film 11, overlay film 12 has good breathability can support high-density cell growth, carefully
Born of the same parents' bag seals.The inner surface of described overlay film 12 is evenly equipped with several projections 2, described protruding 2 hemispherical,
And the radius of a ball of protruding 2 is 0.005 μm.Described bag 1 top is provided with feed tube 3 and quality inspection pipe 4,
One end of described feed tube 3 and quality inspection pipe 4 and extraneous contact is connected to screw-cap 5, seals.
Having the beneficial effects that of the present embodiment: hemispherical protuberances 2 makes cell be easily adapted to the radian of protruding 2, more
Good is attached on bag 1.
Claims (9)
- null1. high efficiency cell culture bag，It is characterized in that: include basement membrane (11)，Described basement membrane (11) Butt sealing composition bag (1)，Overlay film (12) is set in described basement membrane (11)，The inner surface of described overlay film (12) arranges several protruding (2)，Described bag (1) top is provided with feed tube (3) and quality inspection pipe (4)，One end of described feed tube (3) and quality inspection pipe (4) and extraneous contact is connected to screw-cap (5)，Barrier film (6) is set in described bag (1)，Bag (1) is divided into two parts by described barrier film (6)，Described bag (1) above arranges the opening (61) matched with barrier film (6)，From feed tube (3) to barrier film (6) when injecting liquid in bag (1) to bag (1) away from a side shifting of protruding (2) until fitting with bag (1)，When from opening (61) to barrier film (6) when injecting liquid in bag (1) to bag (1) near a side shifting of protruding (2) until fitting with bag (1).
- High efficiency cell culture bag the most according to claim 1, it is characterised in that: described basement membrane (11) and overlay film (12) are fep film.
- High efficiency cell culture bag the most according to claim 1, it is characterised in that: described projection (2) is that point-like is protruding.
- High efficiency cell culture bag the most according to claim 3, it is characterised in that: described projection (2) is hemispherical, half elliptic or triangle.
- High efficiency cell culture bag the most according to claim 1, it is characterised in that: described projection (2) is raised strip.
- High efficiency cell culture bag the most according to claim 5, it is characterised in that: the cross-sectional area of described projection (2) is hemispherical, half elliptic or triangle.
- High efficiency cell culture bag the most according to claim 1, it is characterised in that: described projection (2) is hemispherical, and the radius of a ball of protruding (2) is 0.005 μm.
- High efficiency cell culture bag the most according to claim 1, it is characterised in that: the thickness of described bag (1) is 5-10 μm.
- High efficiency cell culture bag the most according to claim 1, it is characterised in that: check valve (41) is set on described feed tube (3).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620302744.3U CN205710773U (en) | 2016-04-12 | 2016-04-12 | High efficiency cell culture bag |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620302744.3U CN205710773U (en) | 2016-04-12 | 2016-04-12 | High efficiency cell culture bag |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205710773U true CN205710773U (en) | 2016-11-23 |
Family
ID=57310540
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620302744.3U Active CN205710773U (en) | 2016-04-12 | 2016-04-12 | High efficiency cell culture bag |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN205710773U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2018123619A1 (en) * | 2016-12-26 | 2018-07-05 | 東洋製罐グループホールディングス株式会社 | Filter-equipped bag for cells and production method therefor |
| CN111440720A (en) * | 2020-04-17 | 2020-07-24 | 海口健康岛生物科技有限公司 | Immune cell culture device |
-
2016
- 2016-04-12 CN CN201620302744.3U patent/CN205710773U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2018123619A1 (en) * | 2016-12-26 | 2018-07-05 | 東洋製罐グループホールディングス株式会社 | Filter-equipped bag for cells and production method therefor |
| CN111440720A (en) * | 2020-04-17 | 2020-07-24 | 海口健康岛生物科技有限公司 | Immune cell culture device |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20220623 Address after: 361012 unit 431, floor 4, building C, Xiamen international shipping center, No. 93, Xiangyu Road, Xiamen area, China (Fujian) pilot Free Trade Zone, Xiamen, Fujian Patentee after: Xiamen Sany hematopoietic Technology Co.,Ltd. Address before: Room A304, building 10, innovation park, Haixi high tech Industrial Park, Fuzhou high tech Zone, Keji East Road, Shangjie Town, Minhou County, Fuzhou City, Fujian Province 350108 Patentee before: FUJIAN STEMERY TECHNOLOGY CO.,LTD. |