CN213398578U - Trace sample cat parvovirus detection card - Google Patents

Trace sample cat parvovirus detection card Download PDF

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Publication number
CN213398578U
CN213398578U CN202022114370.1U CN202022114370U CN213398578U CN 213398578 U CN213398578 U CN 213398578U CN 202022114370 U CN202022114370 U CN 202022114370U CN 213398578 U CN213398578 U CN 213398578U
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China
Prior art keywords
liquid inlet
sample
puncture
detection
cat
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CN202022114370.1U
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Chinese (zh)
Inventor
孙国龙
任妮妮
梁娜娜
王洪霞
殷柯
孙丽华
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Weifang Dernotek Biotechnology Co ltd
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Weifang Dernotek Biotechnology Co ltd
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Abstract

The utility model discloses a micro sample cat parvovirus detection card, which comprises a shell and a cat parvovirus detection chip arranged at the inner side of the shell; the casing is opposite to a liquid inlet at the liquid inlet of the cat parvovirus detection chip and is opposite to an observation port of a T line and a C line of the cat parvovirus detection chip, and a bulge is integrally formed at the liquid inlet of the casing; the convex part is provided with an open liquid inlet and a closed bin; the bottom of the closed bin is higher than the bottom of the liquid inlet; a liquid inlet hole is formed between the bottom of the closed bin and the liquid inlet; the utility model discloses a trace sample cat parvovirus detects card independently sets up quantitative diluent in the casing, and it can directly dilute on detecting the card, utilizes the puncture stopper can accomplish sample temporary storage, sample dilution and sample advance kind flow operation, its easy operation, and can not cause cross infection.

Description

Trace sample cat parvovirus detection card
Technical Field
The utility model relates to a cat parvovirus detects card, concretely relates to micro sample cat parvovirus detects card belongs to cat parvovirus and detects card technical field.
Background
Infection with feline parvovirus can cause symptoms such as mental depression, anorexia, fever, soft stool or slight vomiting, and the like, and then the symptoms develop into frequent vomiting and severe diarrhea with high infection rate and mortality rate; if the cat is infected with parvovirus, early detection and early treatment are required; wherein, the immunodiagnosis reagent utilizes the mutual combination reaction of antigen and specific antibody to carry out qualitative or quantitative detection, and the immunodiagnosis technology is taken as one of the most sensitive and widely applied micro-scale and ultra-micro-scale diagnosis technologies in modern times and is more and more widely applied to clinical examination and biological research; therefore, the existing feline parvovirus usually adopts a colloidal gold immunochromatography method for detection, but in the prior art, the detection of a trace sample needs to dilute the sample in the early stage so as to detect the sample in the later stage, the operation is more complicated, the diluent is taken according to experience, and in the operation process, the diluent is easily subjected to cross contamination.
Disclosure of Invention
In order to solve the problem, the utility model provides a trace sample cat parvovirus detects card independently sets up quantitative diluent in the casing, and it can directly dilute on detecting the card, easy operation, and can not cause cross infection.
The utility model relates to a micro sample cat parvovirus detection card, which is characterized in that a shell is made into a bulge part, a sampling port and a diluent storage bin (a closed bin) are arranged on the bulge part, the bottom of the closed bin is communicated with the lower part of the sampling port, the closed bin is filled with a certain amount of diluent by utilizing the communication port (liquid inlet hole) and is closed by a puncture plug after the filling is finished, when dilution is needed, the diluent is led into the liquid inlet through the puncture of the puncture needle at the inner side of the detection card outer package, so that the sample loaded on the inner side of the liquid inlet can be diluted, and in order to improve the dilution effect, the bottom of the liquid inlet is provided with another puncture plug for sealing, when dilution is finished, the sample can be led into the cat parvovirus detection chip through the puncture needle for detection, whether the sample contains the feline parvovirus is judged by observing the T line and the C line of the feline parvovirus detection chip, and the structure is as follows: comprises a shell and a cat parvovirus detection chip arranged on the inner side of the shell; the casing is opposite to a liquid inlet at the liquid inlet of the cat parvovirus detection chip and is opposite to an observation port of a T line and a C line of the cat parvovirus detection chip, and a bulge is integrally formed at the liquid inlet of the casing; the convex part is provided with an open liquid inlet and a closed bin; the bottom of the closed bin is higher than the bottom of the liquid inlet; a liquid inlet hole is formed between the bottom of the closed bin and the liquid inlet; a first puncture plug is arranged in the liquid inlet hole; the bottom of the liquid inlet of the shell is provided with a liquid detection hole communicated with the liquid inlet; a second puncture plug is arranged in the liquid detection hole; the aperture of the bottom of the liquid inlet is smaller than that of the liquid detection hole.
Furthermore, the first puncture plug comprises a pipe body, one end of the pipe body, which is close to the liquid inlet, is coated and bonded with a puncture membrane; the outer part of the puncture membrane of the tube body is sleeved with a rubber outer sleeve; the rubber overcoat and the sealed joint in feed liquor hole, after the puncture membrane was impaled, the diluent was leading-in to the body through the puncture membrane perforation, finally got into the inlet and dilutes, and the rubber overcoat can seal the feed liquor hole.
Still further, a snap ring of a ring and feed liquor hole joint is seted up on the rubber overcoat, seals through snap ring and feed liquor hole, can avoid appearing sealing back axial displacement.
Furthermore, the second puncture plug comprises a puncture membrane which is bonded with the periphery of the bottom of the liquid inlet and is bonded with one end of the tube body; and a rubber outer sleeve embedded with the liquid detection hole is arranged outside the pipe body.
Furthermore, the cat parvovirus detection chip comprises a PVC bottom plate, a sample pad which is arranged on the PVC bottom plate and is composed of glass fiber, polyester fiber or nitrocellulose, a gold-labeled binding pad containing a cat parvovirus specific antibody, a nitrocellulose membrane pressed on the lower part of one end of the gold-labeled binding pad, and a water absorption pad pressed on the upper part of the other end of the nitrocellulose membrane, wherein the nitrocellulose membrane is provided with a detection line coated with a CPV specific antibody paired with a labeled antibody, and a quality control line coated with a goat anti-mouse IgG antibody.
Compared with the prior art, the utility model discloses a trace sample cat parvovirus detects card independently sets up quantitative diluent in the casing, and it can directly dilute on detecting the card, utilizes the puncture stopper can accomplish sample temporary storage, sample dilution and sample advance kind flow operation, its easy operation, and can not cause cross infection.
Drawings
Fig. 1 is a schematic view of the overall structure of the present invention.
Fig. 2 is a schematic structural view of the first puncture plug of the present invention.
Detailed Description
As shown in figures 1 and 2, the micro-sample cat parvovirus detection card is characterized in that a shell is made into a bulge part, a sampling port and a diluent storage bin (closed bin) are arranged on the bulge part, the bottom of the closed bin is communicated with the lower part of the sampling port, the closed bin is filled with quantitative diluent by using a communication port (liquid inlet hole), after filling is completed, the closed bin is closed by a puncture plug, when dilution is needed, the diluent is introduced into a liquid inlet by puncturing a puncture needle on the inner side of the outer package of the detection card, so that the sample loaded on the inner side of the liquid inlet can be diluted, in order to improve the dilution effect, the bottom of the liquid inlet is provided with another puncture plug for closing, after dilution is completed, the sample can be introduced into a cat parvovirus detection chip by the puncture needle for detection, whether the sample contains the cat parvovirus is judged by observing the T line and the C line of the cat parvovirus detection chip, the concrete structure is as follows: comprises a shell 1 and a cat parvovirus detection chip arranged on the inner side of the shell; the cat parvovirus detection chip comprises a shell 1, a liquid inlet 2 and an observation port 3, wherein the shell 1 is opposite to the liquid inlet of the cat parvovirus detection chip and is opposite to the T line and the C line of the cat parvovirus detection chip, and a protruding part 4 is integrally formed at the liquid inlet of the shell 1; the convex part 4 is provided with an open liquid inlet 2 and a closed bin 6; the bottom of the closed bin 6 is higher than the bottom of the liquid inlet 2; a liquid inlet hole is formed between the bottom of the closed bin 6 and the liquid inlet 2; a first puncture plug 7 is arranged in the liquid inlet hole; the bottom of the liquid inlet of the shell 1 is provided with a liquid detection hole 8 communicated with the liquid inlet; a second puncture plug 9 is arranged in the liquid detection hole 8; the aperture of the bottom of the liquid inlet 2 is smaller than that of the liquid detection hole 8.
The first puncture plug 7 comprises a pipe body 71, one end of the pipe body, which is close to the liquid inlet, is coated and bonded with a puncture membrane 72; the tube body 71 is sleeved with a rubber outer sleeve 73 outside the puncture membrane; rubber overcoat 73 and the sealed joint in feed liquor hole, after the puncture membrane is impaled, the leading-in body of diluent through the puncture membrane perforation finally gets into the inlet and dilutes, and the rubber overcoat can seal the feed liquor hole. The rubber jacket 73 is provided with a snap ring 74 clamped with the liquid inlet hole, and the snap ring is sealed with the liquid inlet hole, so that the axial movement after sealing can be avoided. The second puncture plug 9 comprises a puncture film 91 which is bonded with the periphery of the bottom of the liquid inlet and is bonded with one end of the pipe body; the tube 71 is externally provided with a rubber outer cover 73 fitted into the liquid detection hole. The cat parvovirus detection chip comprises a PVC bottom plate 10, a sample pad 11, a gold-labeled binding pad 12, a nitrocellulose membrane 13 and a water absorption pad 14, wherein the sample pad 11 is arranged on the PVC bottom plate and is composed of glass fibers, polyester fibers or nitrocellulose fibers, the gold-labeled binding pad 12 contains a cat parvovirus specific antibody, the nitrocellulose membrane 13 is pressed on the lower portion of one end of the gold-labeled binding pad, the water absorption pad 14 is pressed on the upper portion of the other end of the nitrocellulose membrane, a detection line coated with a CPV specific antibody matched with a labeled antibody and a quality control line coated with a goat anti-mouse IgG antibody are arranged on the nitrocellulose.
The above-mentioned embodiment is only the preferred embodiment of the present invention, so all the equivalent changes or modifications made by the structure, features and principles of the present invention are included in the claims of the present invention.

Claims (3)

1. A micro sample cat parvovirus detection card comprises a shell and a cat parvovirus detection chip arranged on the inner side of the shell; the casing just detects the inlet of its feed liquor department of chip to cat parvovirus to and just detects the viewing aperture of its T line of chip and C line to cat parvovirus, its characterized in that: the shell is integrally provided with a bulge at the liquid inlet; the convex part is provided with an open liquid inlet and a closed bin; the bottom of the closed bin is higher than the bottom of the liquid inlet; a liquid inlet hole is formed between the bottom of the closed bin and the liquid inlet; a first puncture plug is arranged in the liquid inlet hole; the bottom of the liquid inlet of the shell is provided with a liquid detection hole communicated with the liquid inlet; a second puncture plug is arranged in the liquid detection hole; the aperture of the bottom of the liquid inlet is smaller than that of the liquid detection hole; the first puncture plug comprises a pipe body, one end of the pipe body, which is close to the liquid inlet, is coated and bonded with a puncture film; the outer part of the puncture membrane of the tube body is sleeved with a rubber outer sleeve; the rubber outer sleeve is hermetically clamped with the liquid inlet hole; the second puncture plug comprises a puncture membrane which is bonded with the periphery of the bottom of the liquid inlet and is bonded with one end of the pipe body; and a rubber outer sleeve embedded with the liquid detection hole is arranged outside the pipe body.
2. The micro-sample feline parvovirus detection card according to claim 1, characterized in that: the rubber jacket is provided with a snap ring which is clamped with the liquid inlet hole.
3. The micro-sample feline parvovirus detection card according to claim 1, characterized in that: the cat parvovirus detection chip comprises a PVC bottom plate, a sample pad, a gold-labeled combination pad, a nitrocellulose membrane and a water absorption pad, wherein the sample pad is arranged on the PVC bottom plate and is composed of glass fibers, polyester fibers or nitrocellulose fibers, the gold-labeled combination pad contains a cat parvovirus specific antibody, the nitrocellulose membrane is pressed on the lower portion of one end of the gold-labeled combination pad, the water absorption pad is pressed on the upper portion of the other end of the nitrocellulose membrane, a detection line coated with a CPV specific antibody matched with a labeled antibody and a quality control line coated with a goat anti-mouse IgG antibody are arranged on the nitrocellulose membrane.
CN202022114370.1U 2020-09-24 2020-09-24 Trace sample cat parvovirus detection card Active CN213398578U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202022114370.1U CN213398578U (en) 2020-09-24 2020-09-24 Trace sample cat parvovirus detection card

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202022114370.1U CN213398578U (en) 2020-09-24 2020-09-24 Trace sample cat parvovirus detection card

Publications (1)

Publication Number Publication Date
CN213398578U true CN213398578U (en) 2021-06-08

Family

ID=76182000

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202022114370.1U Active CN213398578U (en) 2020-09-24 2020-09-24 Trace sample cat parvovirus detection card

Country Status (1)

Country Link
CN (1) CN213398578U (en)

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