CN209791574U

CN209791574U - ELISA chip

Info

Publication number: CN209791574U
Application number: CN201822119020.7U
Authority: CN
Inventors: 谢孝民; 孙玉平; 郭金城
Original assignee: Suzhou Wen Hao Microfluidic Technology Ltd By Share Ltd
Current assignee: Suzhou Wen Hao Microfluidic Technology Ltd By Share Ltd
Priority date: 2018-12-17
Filing date: 2018-12-17
Publication date: 2019-12-17
Anticipated expiration: 2028-12-17

Abstract

The utility model discloses an ELISA chip belongs to biological detection technical field. The chip of the utility model comprises a chip body, wherein the chip body comprises a first surface and a second surface which are oppositely arranged, a plurality of reactors are arranged between the first surface and the second surface, and the reactors are blind holes arranged from the first surface to the second surface; the reactor comprises a first cylindrical section and a second cylindrical section which are coaxially arranged, the inner diameter of the first cylindrical section is smaller than that of the second cylindrical section, the top surface of the first cylindrical section is overlapped with the first surface and is arranged as an opening, and the opening is arranged as a sample port; the second surface is equipped with miniflow channel and liquid outlet, and miniflow channel is linked together with a plurality of second cylinder section bottoms, and miniflow channel one end is connected with the liquid outlet, and the liquid outlet is connected with the pump. The utility model discloses not only can reach the envelope area that increases antigen or antibody, can also play and reduce the influence of external light source to the detection.

Description

ELISA chip

Technical Field

The utility model relates to an ELISA chip belongs to biological detection technical field.

background

ELISA is an Immunoassay (IA) based on immobilization of an antigen or antibody and enzymatic labeling of the antigen or antibody. After the substrate of the enzyme reaction is added, the substrate is catalyzed by the enzyme to form a colored product, and the amount of the product is directly related to the amount of the detected substance in the sample, thereby carrying out qualitative or quantitative analysis. Because various antigen components, including small molecule hapten, can be used to prepare specific antiserum or monoclonal antibody, and the antibody can be used as reagent to detect corresponding antigen in specimen, the application range of immunoassay is very wide.

however, the reaction tube of the traditional ELISA chip is a straight tube type, the coating area of the antigen or antibody is small, and traditionally, a third-party liquid adding device is needed to be used for adding liquid into the reaction tube from the upper part, and then the liquid is taken away by the third-party liquid adding device from the upper part, so that the operation is complex, the waste liquid treatment is not clean, and the detection effect is influenced.

SUMMERY OF THE UTILITY MODEL

in order to solve the technical problem, the utility model provides an ELISA chip and application method thereof sets up the miniflow channel in the chip lower part, takes away liquid from the chip lower part through the pump, and after setting up through the aforesaid, can further set the reactor to inside cascaded tubular structure that is, not only can reach the envelope area that increases antigen or antibody, can also play and reduce the influence of external light source to the detection.

The first purpose of the utility model is to provide an ELISA chip, which comprises a chip body,

The chip body comprises a first surface and a second surface which are oppositely arranged, a plurality of reactors are arranged between the first surface and the second surface, and the reactors are blind holes arranged from the first surface to the second surface;

The reactor comprises a first cylindrical section and a second cylindrical section which are coaxially arranged, the inner diameter of the first cylindrical section is smaller than that of the second cylindrical section, the top surface of the first cylindrical section is overlapped with the first surface and is arranged as an opening, and the opening is arranged as a sample injection port;

The second surface is equipped with miniflow channel and liquid outlet, miniflow channel be linked together with a plurality of second cylinder section bottoms, and miniflow channel one end is connected with the liquid outlet, the liquid outlet is connected with the pump, can use the pump to take away the liquid in the reactor.

Further, the diameter of the first cylindrical section is 0.5-6 mm.

Further, the diameter of the second cylindrical section is 0.6-50 mm.

further, the first surface is made of opaque materials. Further preferably a black material.

furthermore, the second surface is prepared by adopting a transparent material. The material arrangement of first surface and second surface is favorable to the light path of whole chip to be gathered.

Further, the reactors are arranged linearly on the chip. Is favorable for automatic sample adding setting.

Furthermore, the micro-channel is arranged in a tree-shaped distribution manner, the reactor is arranged at the tail end of a branch of the micro-channel, and the liquid outlet is arranged at the trunk end of the micro-channel.

The utility model has the advantages that:

The utility model discloses set up the miniflow channel in the chip lower part, take away liquid from the chip lower part through the pump, convenient operation reduces the operational risk, and after setting up through the aforesaid, can further set the reactor to inside cascaded tubular structure that is, not only can reach the envelope area that increases antigen or antibody, can also play the influence that reduces external light source to the detection.

Drawings

FIG. 1 is a schematic diagram of a prior art reactor;

FIG. 2 is a schematic structural diagram of the chip of the present invention;

FIG. 3 is a schematic structural view of the chip reactor of the present invention;

Fig. 4 is a schematic view of a first surface of the chip of the present invention;

FIG. 5 is a schematic diagram of a reactor of the present invention containing coated antigens or antibodies;

Fig. 6 is a schematic diagram of the photoelectric detection of the reactor of the present invention.

Detailed Description

The present invention is further described with reference to the following drawings and specific embodiments so that those skilled in the art can better understand the present invention and can implement the present invention, but the embodiments are not to be construed as limiting the present invention.

Example 1:

with reference to fig. 2 and 3, the ELISA chip of the present invention comprises a chip body 1, wherein the chip body 1 comprises a first surface 2 and a second surface 3 which are oppositely arranged, a plurality of reactors 4 are arranged between the first surface 2 and the second surface 3, the reactors 4 are blind holes arranged from the first surface 2 to the second surface 3, the first surface is provided with an opening, and the opening is arranged as a sample injection port 43; the second surface is provided with a seal; the reactor 4 comprises a first cylindrical section 41 and a second cylindrical section 42 which are coaxially arranged, the inner diameter of the first cylindrical section 41 is smaller than that of the second cylindrical section 42, the diameter of the first cylindrical section 41 is 0.5-6 mm, and the diameter of the second cylindrical section 42 is 0.6-50 mm; the second surface 3 is provided with a micro-channel 5 and a liquid outlet 6, the micro-channel 5 is communicated with the bottoms of the plurality of second cylindrical sections 42, one end of the micro-channel 5 is connected with the liquid outlet 6, and the liquid outlet 6 is connected with a pump, so that liquid in the reactor 4 can be pumped away by the pump.

The utility model discloses a setting of first cylinder section and second cylinder section not only can increase the envelope area of antigen or antibody, can also play and reduce the influence of other external light sources to the testing result, see figure 6, as shown in figure 6: the ordinary reactor can not block the interference light 10, the light signal detector 8 not only absorbs the light emitted by the normal light source 9, but also the interference light 10 is absorbed by the light signal detector 8; and the utility model discloses a diameter of first cylinder section is less than second cylinder section, and the disturbing light 10 that shines into from the side will be blocked by first cylinder section, can not absorb by light signal detector 8, can play the effect of shielding interference.

The utility model discloses first surface 2 adopts the preparation of opaque black material to form, and second surface 3 adopts the preparation of transparent material to form, and the material setting on first surface and second surface is favorable to the light path collection of whole chip.

The reactor 4 of the utility model is arranged on the chip according to the linear mode. Is favorable for automatic sample adding setting.

The utility model discloses a miniflow channel 5 adopts tree-shaped distribution to set up, and reactor 4 sets up at miniflow channel 5's branch end, and liquid outlet 6 sets up the trunk end at miniflow channel 5.

When in use, the utility model discloses a method for using chip, including following step: adding antigen, antibody, coating liquid, confining liquid or washing liquid into the reactor through the sample adding port, and removing the reacted or washed liquid through the micro-flow channel.

specifically, when the indirect method is adopted, the method specifically comprises the following steps:

(1) diluting the antigen with a coating solution, adding the antigen into the reactor through a sample adding port, and coating for 0.5-12 h;

(2) adding a washing solution into the reactor through the sample adding port, removing the washing solution through the micro-channel after washing, and washing for 2-3 times;

(3) Adding confining liquid into the reactor through the sample adding port, sealing for a period of time, and removing the confining liquid through the micro-channel;

(4) Adding a sample to be detected or a control sample into the reactor through the sample adding port for incubation; washing by adopting the washing method in the step (2);

(5) adding an enzyme-labeled antibody into the reactor through a sample adding port for incubation, and then washing by adopting the washing method in the step (2);

(6) Adding a substrate color developing solution into the reactor through the sample adding port, adding a stop solution after reaction, and then judging.

The above-mentioned embodiments are merely preferred embodiments for fully illustrating the present invention, and the scope of the present invention is not limited thereto. Equivalent substitutes or changes made by the technical personnel in the technical field on the basis of the utility model are all within the protection scope of the utility model. The protection scope of the present invention is subject to the claims.

Claims

1. An ELISA chip is characterized by comprising a chip body,

The second surface is equipped with miniflow channel and liquid outlet, miniflow channel be linked together with a plurality of second cylinder section bottoms, and miniflow channel one end is connected with the liquid outlet, the liquid outlet is connected with the pump.

2. The ELISA chip of claim 1 wherein the first cylindrical section has a diameter of 0.5 to 6 mm.

3. The ELISA chip of claim 1 wherein the second cylindrical section has a diameter of 0.6 to 50 mm.

4. The ELISA chip of claim 1, wherein said first surface is made of an opaque material.

5. the ELISA chip of claim 1 wherein the second surface is made of a transparent material.

6. the ELISA chip of claim 1 wherein the reactors are arranged linearly on the chip.

7. The ELISA chip of claim 1 wherein the microchannel is arranged in a tree, the reactor is located at the end of the branch of the microchannel, and the outlet is located at the main end of the microchannel.