CN206740785U - A kind of quantitative blood and haemocyte filtering sample-adding is used to detect syphilis antibody device - Google Patents
A kind of quantitative blood and haemocyte filtering sample-adding is used to detect syphilis antibody device Download PDFInfo
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- CN206740785U CN206740785U CN201720568959.4U CN201720568959U CN206740785U CN 206740785 U CN206740785 U CN 206740785U CN 201720568959 U CN201720568959 U CN 201720568959U CN 206740785 U CN206740785 U CN 206740785U
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Abstract
The utility model provides a kind of quantitative blood and haemocyte filtering sample-adding and is used to detect syphilis antibody device, including bottle, dropper, and described dropper is fixed on described bottle；Described bottle includes body, bottleneck, bottle cap, and described bottleneck is fixed on the top of described body, and described bottle cap is engaged with described bottleneck, and described bottle cap is provided with through hole, and described dropper is through the through hole and is fixed on described bottle cap；Described dropper includes liquid storing part, imbibition portion, some cryptomere portions, some necks, is connected between described cryptomere portion by neck, one end of described liquid storing part is connected with described cryptomere portion, and the other end is connected with described imbibition portion.Quantitative blood and haemocyte filtering sample-adding described in the utility model solves the problems, such as that trace sample is added dropwise without standard measure for detecting syphilis antibody device, suitable for small space quantitative sampling, solution scene takes blood, and haemocyte filter effect is bad to influence experimental result interpretation.
Description
Technical field
The utility model belongs to medical instruments field, is used for more particularly, to a kind of quantitative blood and haemocyte filtering sample-adding
Detect syphilis antibody device.
Background technology
Syphilis is the infectious disease as caused by infection by Treponema pallidum.China is passed to from 16 beginnings of the century, turns into a kind of fast at that time
Fast popular disease.In recent decades, because venereal disease is widely current, patients with syphilis also increasingly increases.The course of disease is overflow after syphilis illness
Long, early stage invades genitals and skin, and late period invades each organ of whole body, produces diversified sings and symptoms, lesion is almost
The each internal organs of whole body can be involved.Syphilis can mutually be propagated by sexual behaviour in crowd, and can be transmitted to fetus by mother,
Jeopardize the next generation.Only a few patient is infected by the be infectious articles for daily use of infringement patient of kiss, lactation, contact.Property
In spreading disease, the number of patients of syphilis is low, but because its course of disease is grown, harmfulness is big, should pay attention to.
After microspironema pallidum invades human body, the specificity of the anti-microspironema pallidum of immune system generation of people can be stimulated to resist
Body, using colloid gold immune detection technique, the syphilis helicoid antibody that can be quickly detected in people's blood, diagnosis is provided for clinic
And prevention, suitable for the primary dcreening operation that venereal disease, people at highest risk, Voluntary blood donor are live.
Syphilis helicoid antibody detection kit application colloid gold immune detection technique and chromatographic theory, the syphilis in sample
Antibody forms antigen antibody complex with colloid gold label antigen binding, because chromatography effect compound moves forward along paper slip,
By formed during detection line with pre-coated antigen binding " AuTP-Ag-TP Ab-TP Ag " centre-fills and aggegation develops the color, dissociate
Colloid gold label Treponema pallidum antigen then at control line with the pre-coated anti-TP antibody of mouse and be enriched with colour developing, ' negative ' specimens then only exist
Developed the color at control line.
In medical treatment detection or test in laboratory, the instrument of dropper right and wrong usually.In common medical treatment detection, operation
Personnel often need to use dropper that serum, blood plasma and whole blood equal samples is added dropwise, although the volume difference that existing dropper often drips is little,
It is that can not be accurate to half drop or other volumes, because many products are controlled to sample-adding amount, current existing dropper can not
Meet the control to the accurate sample-adding of various volumes, can so influence the accuracy of testing result.Detection project of the same race is sometimes
A variety of samples can be detected, such as serum, blood plasma or whole blood, because the amount containing serum is different in the whole blood of same volume
, by the whole blood and serum or blood plasma that cause same volume, the amount of material to be detected is inconsistent, it is however generally that 2 times of volumes
The amount of contained test substance is approximate with content in the serum or blood plasma of 1 times of volume in whole blood, therefore often can be appreciated that some products are said
Pointed out in bright book, the product can add 10 microlitres of serum or blood plasma, 20 microlitres of whole blood.Due to 10 microlitres or 20 microlitres of whole bloods or blood
Clear or blood plasma, drop-wise drippage is not easy into, it is necessary to touch detection reagent or detection means, thus needs to touch in dropper
Detection reagent or detection means, so there is the risk of pollution, so taking for 2 times drop of blood to add the such operation of whole blood, it is necessary to consume 2
Individual dropper, cause to waste.
Lateral chromatography detection in, whole blood sample compared with serum and plasma sample have the advantages that it is convenient with it is timely.Serum and blood
The preparation of slurry, it is required to wait certain time, if left standing for dozens of minutes, at most 2-4 hours at least, can be with if centrifugation
Shorten to 10 minutes, but need special installation and supporting consumptive material.And amount for taking blood at least also wants 1-2ml or more.Such operation
The problem of existing has：
1. than relatively time-consuming, typically 1-2 hours are about needed just to take testing result, or dependent on special instrument；
2. amount for taking blood is larger；
3. being unfavorable for Site Detection, detect whole blood or refer to that a blood is then advantageous to field quick detection, be some acute
Disease (such as myocarditis disease) strives for valuable treatment time.But whole blood can influence testing result in detection, current at present
Way is that the material of some reagents or blood cell filtration is added in detection means, to remove the interference of red blood cell in whole blood,
The red blood cell that such method is accumulated can also be had an impact to the flowing of liquid, and the red blood cell accumulated is easily broken,
The hemochrome discharged after erythroclasis can also be impacted to detection, and then testing result is had an impact.
The content of the invention
In view of this, the utility model is directed to a kind of quantitative blood and haemocyte filtering sample-adding and is used to detecting syphilis resist
Body device, solve the problems, such as that trace sample is added dropwise without standard measure, solve the sample that same dropper can add a variety of different volumes,
Suitable for small space (heparin tube) quantitative sampling, available for the antibody test relevant item in detection whole blood sample.
To reach above-mentioned purpose, what the technical solution of the utility model was realized in：
A kind of quantitative blood and haemocyte filtering sample-adding is used to detect syphilis antibody device, including bottle, dropper, described
Dropper is fixed on described bottle；
Described bottle includes body, bottleneck, bottle cap, and described bottleneck is fixed on the top of described body, described
Bottle cap is engaged with described bottleneck, and described bottle cap is provided with through hole, and described dropper is through the through hole and is fixed on described
Bottle cap on；
Described dropper includes liquid storing part, imbibition portion, some cryptomere portions, some necks, between described cryptomere portion
It is connected by neck, one end of described liquid storing part is connected with described cryptomere portion, the other end and described imbibition portion phase
Connection.
Further, sealing rubber ring is provided with the outside of described dropper, described dropper passes through described caulking gum
Circle is fixed on described bottle cap.
Further, described bottle cap is threadedly coupled with described bottleneck.
Further, the volume sum in described cryptomere portion is less than the volume of described liquid storing part.
Further, the volume in described cryptomere portion is gradually reduced successively；Wherein, the cryptomere being connected with described liquid storing part
The volume in portion is maximum.
Further, the quantity of described neck is 1, and the quantity in cryptomere portion is 2, respectively the first cryptomere portion, second
Cryptomere portion, the first described cryptomere portion are located at the top in the second described cryptomere portion, and the first cryptomere portion passes through with the second cryptomere portion
Neck is connected, and the second described cryptomere portion is connected with described liquid storing part.
Further, the volume in the second described cryptomere portion is more than the volume in the first described cryptomere portion.
Further, described body is provided with well, and hemofiltration film is provided with the well.
Relative to prior art, quantitative blood and haemocyte filtering sample-adding described in the utility model resists for detecting syphilis
Body device has the advantage that：
(1) quantitative blood and haemocyte filtering sample-adding described in the utility model is micro- for detecting the solution of syphilis antibody device
The problem of amount sample is added dropwise without standard measure, solves the sample that same dropper can add 2 kinds of different volumes, suitable for small space
(heparin tube) quantitative sampling, solution scene take blood, and haemocyte filter effect is bad to influence experimental result interpretation.
(2) quantitative blood and haemocyte filtering sample-adding described in the utility model is provided with for detecting syphilis antibody device
Hemofiltration film, it can will remove red blood cell this step and become pre-treatment, the blood constituent for getting rid of the interfering materials such as red blood cell is added
Enter into detecting instrument or reagent, thus got rid of most interfering material, and then to improve accuracy rate, reduce dry
Disturb, guarantee is provided for the accuracy of detection.
Brief description of the drawings
Form a part of accompanying drawing of the present utility model to be used for providing further understanding to of the present utility model, this practicality is new
The schematic description and description of type is used to explain the utility model, does not form to improper restriction of the present utility model.
In accompanying drawing：
Fig. 1 is used to detect syphilis antibody dress for the quantitative blood described in the utility model embodiment and haemocyte filtering sample-adding
The schematic diagram put；
Fig. 2 a-2d are that the utility model uses result judgement schematic diagram.
Description of reference numerals：
1- bodies；2- bottlenecks；3- bottle caps；The first cryptomeres of 4- portion；5- necks；The second cryptomeres of 6- portion；7- liquid storing parts；8- inhales
Liquid portion；9- sealing rubber rings；10- wells；11- hemofiltration films.
Embodiment
It should be noted that in the case where not conflicting, the feature in embodiment and embodiment in the utility model can
To be mutually combined.
In description of the present utility model, it is to be understood that term " " center ", " longitudinal direction ", " transverse direction ", " on ", " under ",
The orientation or position relationship of the instruction such as "front", "rear", "left", "right", " vertical ", " level ", " top ", " bottom ", " interior ", " outer " are
Based on orientation shown in the drawings or position relationship, it is for only for ease of description the utility model and simplifies description, rather than instruction
Or imply that signified device or element must have specific orientation, with specific azimuth configuration and operation, therefore be not understood that
For to limitation of the present utility model.In addition, term " first ", " second " etc. are only used for describing purpose, and it is not intended that instruction
Or imply relative importance or the implicit quantity for indicating indicated technical characteristic.Thus, " first ", " second " etc. are defined
Feature can express or implicitly include one or more this feature.In description of the present utility model, unless separately
It is described, " multiple " are meant that two or more.
, it is necessary to which explanation, unless otherwise clearly defined and limited, term " are pacified in description of the present utility model
Dress ", " connected ", " connection " should be interpreted broadly, for example, it may be fixedly connected or be detachably connected, or integratedly
Connection；Can be mechanical connection or electrical connection；Can be joined directly together, can also be indirectly connected by intermediary,
It can be the connection of two element internals.For the ordinary skill in the art, on being understood by concrete condition
State concrete meaning of the term in the utility model.
As shown in figure 1, a kind of quantitative blood and haemocyte filtering sample-adding are used to detect syphilis antibody device, including bottle,
Dropper, described dropper are fixed on described bottle；
Described bottle includes body 1, bottleneck 2, bottle cap 3, and described bottleneck 2 is fixed on the top of described body 1, institute
The bottle cap 3 stated is engaged with described bottleneck 2, and described bottle cap 3 is provided with through hole, and described dropper passes through the through hole and fixation
In on described bottle cap 3；
Described dropper includes liquid storing part 7,8,2, imbibition portion cryptomere portion, neck 5, passes through neck between described cryptomere portion
Portion 5 is connected, and one end of described liquid storing part 7 is connected with described cryptomere portion, and the other end is connected with described imbibition portion 8
It is logical.
Sealing rubber ring 9 is provided with the outside of described dropper, described dropper is fixed by described sealing rubber ring 9
In on described bottle cap 3.
Described bottle cap 3 is threadedly coupled with described bottleneck 2.
The volume sum in described cryptomere portion is less than the volume of described liquid storing part 7.
The volume in described cryptomere portion is gradually reduced successively；Wherein, the appearance in the cryptomere portion being connected with described liquid storing part 7
Product is maximum.
The quantity in described cryptomere portion is 2, and the quantity of neck 5 is 1, respectively the first cryptomere portion 4, the second cryptomere
Portion 6, the first described cryptomere portion 4 are located at the top in the second described cryptomere portion 6, and the first cryptomere portion 4 passes through with the second cryptomere portion 6
Neck 5 is connected, and the second described cryptomere portion 6 is connected with described liquid storing part 7.
The volume in the second described cryptomere portion 6 is more than the volume in the first described cryptomere portion 4.
Described body 1 is provided with well 10, and hemofiltration film 11 is provided with the well 10.
The size of cryptomere portion inner space is equal with liquid absorption, and material is thin plastics, and thickness 0.2mm is convenient to be extruded and determine
Amount.The inner space of first cryptomere portion 4 is 10 microlitres, the inner space of the second cryptomere portion 6 is 20 microlitres.Neck 5 plays connection function,
Thickness 1mm.Liquid storing part 7 internal diameter 3mm, long 5cm is to store suction sample.The internal diameter 1mm of imbibition portion 8, to adapt to the liquid of small size
Dripped into drop-wise, long 2cm is to adapt to the sampling work of the exiguous spaces such as heparin tube.The material of body 1 is thin plastics, thickness 0.5mm
Convenient extruding.There is hemofiltration film 11 on body 1.
In example below, the experimental method of unreceipted actual conditions, generally according to normal condition, or according to manufacturer
Proposed condition.
Describe the utility model in detail below with reference to the accompanying drawings and in conjunction with the embodiments.
The preparation of the collaurum of embodiment 1
The heating of 0.02% chlorauric acid solution is boiled, is rapidly added 2% trisodium citrate 2mL；Solution colour is by light blue
Discoloration is dark blue, continuously adds and claret occurs, continues to boil 10min；There is transparent claret, stop heating, continue to stir
Mix to room temperature, obtain colloidal gold solution of the particle diameter in 20nm or so.Electronic Speculum microscopy, it is ensured that the gold grain of preparation makes its size as far as possible
Otherwise consistent and uniform, particle diameter are remake in 20nm or so.
The preparation of colloidal gold pad of the embodiment 2 containing labelled antibody
1.5mL test tubes are taken, add 1mL colloidal gold solutions；Appropriate Tris buffer solutions are added thereto, and pH is adjusted to 8.0；
15 μ g/mL TP labelled antibodies are added, reach minimum protein concentration, are mixed, in quick oscillation 30min on shaker；Add
10 μ L 10%BSA, mix, vibrate 20min；The 9000rpm in centrifuge, 20min is centrifuged, gently absorbs supernatant；Use 1mL
The loose collaurum precipitation of wash buffer solution resuspensions；Preparing OD (optical density) value at 540nm with fix buffer is
1.4-1.6 colloid gold immune compound；
By above-mentioned colloid gold immune compound and fix buffer according to volume ratio 1:3 ratio mixing, with 2.5 μ l/mm
Amount on glass fibre membrane point sample, form colloidal gold pad, and dry under appropriate conditions, generally 37 DEG C dryings 12 are small
When.
The coating of the nitrocellulose NC films of embodiment 3
Using spray film instrument, 1.0mg/ml TP note antibody is uniformly drawn into the shape on nitrocellulose NC films with 0.001ml/cm
Into T lines；2.0mg/ml sheep anti mouses polyclonal antibody is uniformly drawn to the formation C lines on nitrocellulose NC films with 0.001ml/cm,
And between two lines at intervals of 0.4cm.Drying room is then placed in dry under suitable temperature and humidity.
The assembling of the Test paper of embodiment 4
In the suitable hothouse of condition, what coating that colloidal gold pad and embodiment 3 that embodiment 2 is obtained obtain was completed
Nitrocellulose NC films and sample pad and blotting paper are cut into suitable size, are bonded according to the structure shown in Fig. 1, control
Colloidal gold pad processed is away from, at intervals of 0.4-0.6cm, the interval can be coated with the scribing position of NC films by controlling, adjust between T lines
Cut size and bonding portion size realize that colloidal gold pad and the bonding portion of NC films are not less than the 1/7 of colloidal gold pad, glue
The bonding portion of body gold pad and sample pad is the 1/7-1/2 of colloidal gold pad, and the bonding portion of blotting paper and NC films is not less than water suction
The 1/10 of paper.The above-mentioned each several part being mutually bonded can be fixed on a loading plate in advance or after bonding, and the loading plate can be with
For plastic plate.Suitable size is cut to after the completion of fixation.The Test paper can with so that load above-mentioned hair
It is bright to mention.
The quantitative dropper structure of embodiment 5
1st, it is made up of the first cryptomere portion 4, the second cryptomere portion 6, liquid storing part 7, imbibition portion 8 and neck 5.
2nd, the first cryptomere portion 4, material are thin plastics, and thin plastic thickness 0.2mm, inner space is 10 microlitres.
3rd, the second cryptomere portion 6, material are thin plastics, and thin plastic thickness 0.2mm, inner space is 20 microlitres.
4th, neck 5, connection cryptomere portion 1 and the part of cryptomere portion 2, material are plastics, plastic thickness 1mm.Length is 5mm.Even
Connect the liquid storing part part of cryptomere portion 2, material is plastics, plastic thickness 1mm.Length is 5mm.
5th, liquid storing part 7, material are plastics, plastic thickness 1mm, and internal diameter 3mm, long 5cm are to store suction sample.
6th, imbibition portion 8, material are plastics, plastic thickness 1mm, and internal diameter 1mm, long 2cm are to adapt to the exiguous spaces such as heparin tube
Sampling work.
The quantitative dropper of embodiment 6 uses
When needing to draw 10 microlitres of serum, the first cryptomere 4 is pressed, whole air in the first cryptomere 4 are emptied, by imbibition
Portion 8 is inserted into serum, unclamps the first cryptomere 4, and serum is drawn into imbibition portion 8, and now volume is 10 microlitres, by imbibition portion 8
When lower end moves on to sample application zone, the first cryptomere 4 is pressed again, empties whole air in the first cryptomere 4, now, 10 microlitres of serum
It is transferred completely into sample application zone.
When needing to draw 20 microlitres of whole bloods, the second cryptomere portion 6 is pressed, empties whole air in the second cryptomere portion 6, will
Imbibition portion 8 is inserted into whole blood, unclamps the second capsule portion shape 6, and whole blood is drawn into imbibition portion 8, and now volume is 20 microlitres, will be inhaled
When the lower end of liquid portion 8 moves on to sample application zone, the second cryptomere portion 6 is pressed again, empties whole air in the second cryptomere portion 6, now, 20
Microlitre whole blood is transferred completely into sample application zone.
The use of the drop bottle of embodiment 7
10 microlitres or 20 microlitres of whole blood is taken, is added on well 10, by the filtering of hemofiltration film 11, enters to body 1
In, add 100 microlitres of buffer solutions, after mixing, you can using dropper by sample drop in detection card well in tested.
The Cleaning Principle of embodiment 8 and method
The principle that make use of double-antibody sandwich in immunochromatographic assays to develop the color in the invention, when containing in sample to be tested
When having certain density TP, after being added into well, sample is with mouse source TP in the liquid dissolving colloidal gold pad in dilution
Monoclonal antibody, and TP and the colloid gold label mouse source TP monoclonal antibodies are specifically bound, formation antigen-anti-
Body-colloidal gold composite, and continue to move to detection line by capillarity.When sample reaches detection line, above-mentioned antigen-anti-
Body-colloidal gold composite and the TP antibody for the pairing being coated in detection line are further combined with formation antibody-antigen-antibody-glue
Body gold double-antibody sandwich compound, and assemble colour developing in detection line.With capillarity further along, nature controlling line is reached
When, because TP monoclonal antibodies are mouses in colloidal gold pad, therefore no matter whether contain TP in test plasma, fixed
Capture and develop the color in the sheep anti mouse secondary antibody of nature controlling line, this colour developing ensure that whole reaction system is correct.
During detection, tested sample is instilled in well, T lines (lower section lines), C lines (top lines) are observed after 20min
Colour developing situation.C lines develop the color, T line outlets, show the TP IgG positives in sample (see accompanying drawing 2a)；C lines develop the color, and T lines do not develop the color,
Show that the TP IgG in sample are negative (see accompanying drawing 2b)；C lines do not develop the color (see accompanying drawing 2c-d), show the failure of an experiment, need to survey again
Examination.
Preferred embodiment of the present utility model is the foregoing is only, it is all at this not to limit the utility model
Within the spirit and principle of utility model, any modification, equivalent substitution and improvements made etc., the utility model should be included in
Protection domain within.
Claims (8)
1. a kind of quantitative blood and haemocyte filtering sample-adding are used to detect syphilis antibody device, it is characterised in that：Including bottle, drop
Pipe, described dropper are fixed on described bottle；
Described bottle includes body (1), bottleneck (2), bottle cap (3), and described bottleneck (2) is fixed on described body (1)
Top, described bottle cap (3) are engaged with described bottleneck (2), and described bottle cap (3) is provided with through hole, and described dropper is worn
Cross the through hole and be fixed on described bottle cap (3)；
Described dropper includes liquid storing part (7), imbibition portion (8), some cryptomere portions, some necks (5), described cryptomere portion
Between be connected by neck (5), one end of described liquid storing part (7) is connected with described cryptomere portion, the other end with it is described
Imbibition portion (8) be connected.
2. quantitative blood according to claim 1 and haemocyte filtering sample-adding are used to detect syphilis antibody device, its feature
It is：Sealing rubber ring (9) is provided with the outside of described dropper, described dropper is solid by described sealing rubber ring (9)
Due on described bottle cap (3).
3. quantitative blood according to claim 1 or 2 and haemocyte filtering sample-adding are used to detect syphilis antibody device, it is special
Sign is：Described bottle cap (3) is threadedly coupled with described bottleneck (2).
4. quantitative blood according to claim 1 and haemocyte filtering sample-adding are used to detect syphilis antibody device, its feature
It is：The volume sum in described cryptomere portion is less than the volume of described liquid storing part (7).
5. quantitative blood according to claim 4 and haemocyte filtering sample-adding are used to detect syphilis antibody device, its feature
It is：The volume in described cryptomere portion is gradually reduced successively；Wherein, the appearance in the cryptomere portion being connected with described liquid storing part (7)
Product is maximum.
6. quantitative blood according to claim 1 and haemocyte filtering sample-adding are used to detect syphilis antibody device, its feature
It is：The quantity of described neck (5) is 1, and the quantity in described cryptomere portion is 2, respectively the first cryptomere portion (4), the
Two cryptomere portions (6), the first described cryptomere portion (4) are located at the top in the second described cryptomere portion (6), the first cryptomere portion (4) with
Second (6) portion of cryptomere portion is connected by neck (5), and the second described cryptomere portion (6) is connected with described liquid storing part (7).
7. quantitative blood according to claim 6 and haemocyte filtering sample-adding are used to detect syphilis antibody device, its feature
It is：The volume in the second described cryptomere portion (6) is more than the volume in the first described cryptomere portion (4).
8. quantitative blood according to claim 1 and haemocyte filtering sample-adding are used to detect syphilis antibody device, its feature
It is：Described body (1) is provided with well (10), and hemofiltration film (11) is provided with the well (10).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720568959.4U CN206740785U (en) | 2017-05-19 | 2017-05-19 | A kind of quantitative blood and haemocyte filtering sample-adding is used to detect syphilis antibody device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720568959.4U CN206740785U (en) | 2017-05-19 | 2017-05-19 | A kind of quantitative blood and haemocyte filtering sample-adding is used to detect syphilis antibody device |
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| CN206740785U true CN206740785U (en) | 2017-12-12 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109212185A (en) * | 2018-09-08 | 2019-01-15 | 苏州承美生物科技有限公司 | One-step method pepsinogen Cgene quick detection kit |
-
2017
- 2017-05-19 CN CN201720568959.4U patent/CN206740785U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109212185A (en) * | 2018-09-08 | 2019-01-15 | 苏州承美生物科技有限公司 | One-step method pepsinogen Cgene quick detection kit |
| CN109212185B (en) * | 2018-09-08 | 2022-08-26 | 苏州承美生物科技有限公司 | One-step fast detection kit for pepsinogen I |
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