CN213266543U

CN213266543U - Culture solution supplementing device for cell culture

Info

Publication number: CN213266543U
Application number: CN202022046806.8U
Authority: CN
Inventors: 刘大卫
Original assignee: Henan Zhongke Stem Cell Genetic Engineering Co ltd
Current assignee: Henan Zhongke Stem Cell Genetic Engineering Co ltd
Priority date: 2020-09-17
Filing date: 2020-09-17
Publication date: 2021-05-25
Anticipated expiration: 2030-09-17

Abstract

Cell culture is with culture solution fluid infusion device, including both ends open-ended fluid infusion pipe, the inside slip that the one end of fluid infusion pipe was fixed with end cover and fluid infusion pipe has and sets up the columniform piston with fluid infusion pipe coaxial line, be fixed with the push rod in the one side that the piston deviates from the end cover, the push rod is kept away from the one end of piston and is stretched out fluid infusion pipe and push rod and the coaxial line setting between the piston, be fixed with the drain pipe with fluid infusion pipe intercommunication on the end cover, be fixed with first check valve on the drain pipe, the direction of flow of first check valve is for deviating from the direction of fluid infusion pipe, be fixed with the feed liquor pipe with fluid infusion pipe intercommunication on the fluid infusion pipe, be fixed with the second check valve on the feed liquor pipe, the direction of flow of second check valve is for. Can avoid culture solution and air contact, reduce the condition of cell culture infection, can continuously carry out the fluid infusion to the culture bag simultaneously, convenient to use is swift, excellent in use effect.

Description

Culture solution supplementing device for cell culture

Technical Field

The utility model relates to a stem cell experimental facilities technical field especially relates to cell culture is with culture solution fluid infusion device.

Background

Cell culture techniques, also called cell cloning techniques, are used to obtain large quantities of cells or their metabolites by cell culture. Since biological products are all derived from cells, it can be said that cell culture technology is the most core and basic technology in biotechnology. Cell culture work is widely applied to various fields such as biology, medicine, new drug research and development and becomes one of the most important basic sciences. The growth of cells requires a nutrient environment, and a nutrient medium for maintaining the growth of cells is called a culture medium, which is divided into a culture solution and a culture solid. Wherein, through the in-process of culture solution culture cell, the cell can consume the culture solution, need regularly carry out the fluid infusion operation to it, but present fluid infusion device is all through empting the culture solution toward cylinder inside, and the fluid infusion can be to the inside flow of culture bag, but this kind of mode makes the culture solution expose under the air easily to improve the risk that the cell culture is infected, and the easy unrestrained condition leads to the waste of culture solution. Meanwhile, when liquid is added, the inside of the culture bag is easy to block, so that the culture solution flowing naturally cannot be supplemented normally, and the liquid supplementing process is influenced.

SUMMERY OF THE UTILITY MODEL

To above-mentioned condition, for overcoming prior art's defect, the utility model aims at providing a cell culture is with culture solution fluid infusion device, can reduce the risk that the cell was infected, avoids appearing unrestrained condition simultaneously, improves the efficiency of fluid infusion.

Its solution is, culture solution fluid infusion device for cell culture, including both ends open-ended fluid infusion pipe, the inside slip that the one end of fluid infusion pipe was fixed with end cover and fluid infusion pipe has and sets up the columniform piston with fluid infusion pipe coaxial line, the piston is fixed with the push rod in the one side that deviates from the end cover, the one end that the piston was kept away from to the push rod stretches out the coaxial line setting between fluid infusion pipe and push rod and the piston, be fixed with the drain pipe with fluid infusion pipe intercommunication on the end cover, be fixed with first check valve on the drain pipe, the direction of flow of first check valve is for deviating from the direction of fluid infusion pipe, be fixed with the feed liquor pipe with fluid infusion pipe intercommunication on the fluid infusion pipe, be fixed with the second check valve on the feed liquor pipe, the direction of flow of second check valve is for the direction of being close to the fluid infusion pipe.

Preferably, a push-pull plate is fixed on one end of the push rod far away from the piston.

Preferably, one of feed liquor pipe and drain pipe is kept away from the moisturizing pipe is served and all is fixed with fixed cover, be equipped with the diameter that chamfer and fixed cover were greater than the diameter of drain pipe and feed liquor pipe on a plurality of fixed covers.

Preferably, a plurality of fixed covers all adopt rubber material.

Preferably, a bracket is fixed on the liquid supplementing pipe, and a box body is fixed on the bracket.

Preferably, an opening is arranged at one end of the box body facing the liquid replenishing pipe.

The utility model has the advantages that: can carry out quick fluid replacement when cell culture to can continuously carry out the fluid replacement, improve the efficiency of fluid replacement, avoid the culture solution to expose in the air, thereby the condition of infecting when reducing cell culture. The unrestrained of culture solution when avoiding the fluid replacement can also reduce the pollution to experimental environment when reducing extravagant, the further result of use that improves the device.

Drawings

Fig. 1 is a schematic structural diagram of the whole front shaft side of the present invention.

Fig. 2 is a schematic structural view of the whole back shaft side of the present invention.

Fig. 3 is a schematic view of the overall overlook structure of the present invention.

Fig. 4 is a schematic structural view of the whole front section of the present invention.

Detailed Description

The following describes the embodiments of the present invention in further detail with reference to the accompanying drawings.

As shown in FIGS. 1, 2, 3 and 4, the culture medium replenishing apparatus for cell culture comprises a solution replenishing pipe 1 having openings 13 at both ends, it is characterized in that an end cover 12 is fixed at one end of the liquid supplementing pipe 1, a cylindrical piston 2 which is coaxial with the liquid supplementing pipe 1 is arranged in the liquid supplementing pipe 1 in a sliding way, a push rod 3 is fixed on one surface of the piston 2, which is far away from the end cover 12, one end of the push rod 3, which is far away from the piston 2, extends out of the liquid supplementing pipe 1, the push rod 3 and the piston 2 are coaxially arranged, a liquid outlet pipe 6 communicated with the liquid supplementing pipe 1 is fixed on the end cover 12, a first one-way valve 7 is fixed on the liquid outlet pipe 6, the flowing direction of the first one-way valve 7 is a direction departing from the liquid supplementing pipe 1, a liquid inlet pipe 8 communicated with the liquid supplementing pipe 1 is fixed on the liquid supplementing pipe 1, and a second one-way valve 9 is fixed on the liquid inlet pipe 8, and the flowing direction of the second one-way valve 9 is close to the liquid supplementing pipe 1.

When the device is used, the device is firstly placed at a position needing to work, then the hose on the culture solution bag is communicated with the liquid inlet pipe 8, then the liquid outlet pipe 6 is communicated with the hose on the cell culture bag, then the push rod 3 is pulled towards one end far away from the end cover 12, under the action of the piston 2, negative pressure is formed inside the liquid supplementing pipe 1, so that the culture solution inside the culture solution bag flows towards the inside of the liquid supplementing pipe 1, but at the moment, under the action of the one-way valve, the liquid outlet pipe 6 cannot generate gas flow, so that the culture solution at the moment can only enter the liquid supplementing pipe 1 from the direction of the liquid inlet pipe 8, when the push rod 3 is pushed towards the direction of the end cover 12, the culture solution inside the liquid supplementing pipe 1 cannot flow towards the inside of the liquid inlet pipe 8 and only can flow towards the inside of the liquid outlet pipe 6 under the action of the second one-way valve 9, so that the culture solution flows towards the inside of the culture bag through the liquid outlet pipe, the realization is to the fluid infusion process of culture solution, through promoting and pulling push rod 3, can realize the continuous fluid infusion to the cell culture bag, and convenient and fast has avoided the culture solution to expose in the air simultaneously, has reduced the condition of the infection when cell culture, further improvement the result of use of device.

And a push-pull plate 4 is fixed on one end of the push rod 3 far away from the piston 2.

The push-pull plate 4 is convenient for a user to operate the push rod 3, and the using effect of the device is further improved.

One of feed liquor pipe 8 and drain pipe 6 keep away from liquid supplementing pipe 1 serves and all is fixed with fixed cover 11, be equipped with the diameter that chamfer and fixed cover 11 on a plurality of fixed covers 11 and be greater than the diameter of drain pipe 6 and feed liquor pipe 8.

Through setting up fixed cover 11, during the use, stretch into the hose with fixed cover 11 inside, realize with the sealed of hose to avoid the device gaseous leakage when using, can also avoid breaking away from of feed liquor pipe 8 and drain pipe 6 and hose simultaneously, further improvement the result of use of device.

The fixed sleeves 11 are made of rubber.

The rubber material can further improve the contact sealing with the hose.

A bracket 5 is fixed on the liquid supplementing pipe 1, and a box body 10 is fixed on the bracket 5.

During the use, place the box 10 on support 5 with the culture solution bag, conveniently fix and remove the culture solution bag, effect when further improvement is to the fluid infusion.

An opening 13 is arranged at one end of the box body 10 facing the liquid replenishing pipe 1.

Set up opening 13 on the box 10 and can make things convenient for the hose on the culture solution bag to stretch out and feed liquor pipe 8 between the intercommunication, further improvement device's result of use.

On the premise of conception, various changes and modifications made by the technical scheme of the invention by those skilled in the art should be included in the protection scope determined by the claims of the invention.

Claims

1. Culture solution supplementing device for cell culture, including fluid infusion pipe (1) of both ends opening (13), its characterized in that, the one end of fluid infusion pipe (1) is fixed with end cover (12) and the inside slip of fluid infusion pipe (1) has and sets up columniform piston (2) with fluid infusion pipe (1) coaxial line, be fixed with push rod (3) on the one side that piston (2) deviates from end cover (12), the one end that piston (2) were kept away from in push rod (3) stretches out fluid infusion pipe (1) and push rod (3) and piston (2) between the coaxial line setting, be fixed with drain pipe (6) with fluid infusion pipe (1) intercommunication on end cover (12), be fixed with first check valve (7) on drain pipe (6), the flow direction of first check valve (7) is the direction that deviates from fluid infusion pipe (1), be fixed with feed liquor pipe (8) with fluid infusion pipe (1) intercommunication on fluid infusion pipe (1), and a second one-way valve (9) is fixed on the liquid inlet pipe (8), and the flowing direction of the second one-way valve (9) is close to the liquid supplementing pipe (1).

2. The device for replenishing culture solution to cell culture according to claim 1, wherein a push-pull plate (4) is fixed to an end of the push rod (3) remote from the piston (2).

3. The device for supplementing culture solution to cell culture according to claim 1, wherein fixing sleeves (11) are fixed on the ends of the liquid inlet pipe (8) and the liquid outlet pipe (6) far away from the liquid supplementing pipe (1), chamfers are arranged on the fixing sleeves (11), and the diameter of the fixing sleeves (11) is larger than that of the liquid outlet pipe (6) and the liquid inlet pipe (8).

4. The device for replenishing a culture solution for cell culture according to claim 3, wherein each of the plurality of fixing covers (11) is made of rubber.

5. The device for replenishing a culture medium for cell culture according to claim 1, wherein a holder (5) is fixed to the solution replenishing pipe (1), and a case (10) is fixed to the holder (5).

6. The device for replenishing a culture medium according to claim 5, wherein an opening (13) is provided at an end of the case (10) facing the solution replenishing pipe (1).