CN215480907U - Dual-purpose erlenmeyer flask of microorganism culture, fed-batch inoculation - Google Patents
Dual-purpose erlenmeyer flask of microorganism culture, fed-batch inoculation Download PDFInfo
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- CN215480907U CN215480907U CN202121194740.5U CN202121194740U CN215480907U CN 215480907 U CN215480907 U CN 215480907U CN 202121194740 U CN202121194740 U CN 202121194740U CN 215480907 U CN215480907 U CN 215480907U
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Abstract
The utility model provides a microorganism culture and fed-batch inoculation dual-purpose conical flask, which comprises: the conical flask comprises a conical flask body and a threaded cylindrical bottleneck and can be used for carrying out microorganism culture; the inoculation bottle cap is detachably arranged on the bottle mouth; the bottle cap can be fixed on the bottle mouth through a connecting structure and forms an integral structure with the conical bottle; the inoculation pipe is arranged on the inoculation bottle cap, penetrates through the inoculation bottle cap, one end of the inoculation pipe is positioned in the bottle body, and the other end of the inoculation pipe is connected with the feeding inoculation equipment for feeding inoculation; and the air guide structure is arranged on the inoculation bottle cap, penetrates through the inoculation bottle cap, is used for maintaining the air pressure in the conical bottle, and is provided with a sterilization filter membrane for filtering and sterilizing. The dual-purpose conical flask for microbial culture and fed-batch inoculation provided by the utility model has two functions of microbial culture and fed-batch inoculation, can reduce the possibility of mixed bacteria pollution in the inoculation process, and is suitable for the fields of culture of any microorganisms and fermentation inoculation.
Description
Technical Field
The utility model relates to the technical field of microbial fermentation culture bottles, in particular to a dual-purpose conical bottle for microbial culture and fed-batch inoculation.
Background
The first-stage seed fermentation tank is generally inoculated by two modes, namely a fire ring inoculation method and a pressure difference inoculation method. The fire circle inoculation method is that the seed liquid in the conical flask is directly poured into a fermentation tank through an inoculation port of the fermentation tank containing the fire circle. The inoculation method is very convenient, but has high requirements on the operation of inoculators, and is easy to introduce mixed bacteria along with airflow carelessly, and if the fermentation strain is very fragile and has poor stress resistance and more mixed bacteria exist in the environment, the possibility of causing mixed bacteria pollution by introducing airflow exists no matter how carefully the operators adopt the fire circle method. The pressure difference inoculation method is to inoculate the fermentation liquor in the triangular flask into the fermentation tank through pressure difference, the pressure difference inoculation method needs to transfer the seed liquor to a special inoculation bottle, the transfer process can also increase the risk of contamination, and the pressure resistance of the seed bottle has higher requirements.
Seeds cultured in a common conical flask can only be inoculated by a fire circle method, and the seed liquid in the conical flask can only be transferred to a special inoculating flask by performing pressure difference inoculation or flow addition inoculation, so that the transferring process not only increases the operation steps, but also increases the risk of bacterial contamination. The utility model relates to a microorganism culture and feeding dual-purpose conical flask which can be used for culturing various microorganisms such as aerobic microorganisms and anaerobic microorganisms, and can be directly inoculated in a feeding mode through a peristaltic pump, so that the problem that the inoculation is easy to cause a bacterial contamination risk in the process of fire circle inoculation is solved, and the problem that a pressure difference inoculation flask can be broken due to overlarge pressure is also solved.
SUMMERY OF THE UTILITY MODEL
Aiming at the defects of the prior art, the utility model provides the conical flask for microorganism culture and fed-batch inoculation, which can reduce the risk of mixed bacteria pollution during inoculation and can be used for inoculation by directly adopting a peristaltic pump fed-batch mode.
The utility model adopts the following technical scheme for solving the technical problems:
the utility model provides a microorganism culture and fed-batch inoculation dual-purpose conical flask, which comprises:
the conical flask comprises a conical flask body and a columnar bottle mouth and is used for carrying out microbial culture;
the inoculation bottle cap is detachably arranged on the bottle mouth; can be fixed on the bottle mouth through a connecting structure and forms an integral structure with the conical bottle;
the inoculation pipe is arranged on the inoculation bottle cap and penetrates through the inoculation bottle cap, one end of the inoculation pipe is positioned in the bottle body, and the other end of the inoculation pipe is connected with the feeding inoculation equipment for feeding inoculation; and
and the air guide structure is arranged on the inoculation bottle cap, penetrates through the inoculation bottle cap, has one end positioned in the bottle body and the other end positioned outside, and is used for maintaining the air pressure in the conical bottle.
Further, the connection structure includes:
the external thread is arranged on the outer surface of the bottle mouth; and
the internal thread is arranged on the inner surface of the side wall of the inoculation bottle cap and matched with the external thread.
Further, the inoculation pipe includes:
the first metal tube is arranged on the inoculation bottle cap and penetrates through the inoculation bottle cap;
the first rubber hose is arranged at the lower end of the first metal pipe and is positioned in the bottle body; and
and the second rubber hose is arranged at the upper end of the first metal pipe and is connected with the feeding inoculation equipment.
Furthermore, one end of the second rubber hose, which is far away from the first metal pipe, is also provided with a metal needle.
Further, the gas directing structure comprises:
the second metal tube is arranged on the inoculation bottle cap and penetrates through the inoculation bottle cap, and the lower end of the second metal tube extends into the bottle body;
the third rubber hose is arranged at the upper end of the second metal pipe and is communicated with the outside; and
and the sterilization component is arranged on the third rubber hose and used for preventing external bacteria from entering the bottle body.
Further, the sterilization part is a sterilization filter membrane.
Further, the device also comprises a rubber gasket which is detachably arranged in the inoculation bottle cap; two through holes are arranged on the rubber gasket and used for enabling the inoculation tube and the air guide structure to penetrate through.
Further, the inoculation bottle also comprises a metal gasket which is detachably arranged in the inoculation bottle cap; the metal gasket is provided with two through holes for the seed receiving pipe and the air guide structure to pass through.
Further, a rubber gasket is positioned between the metal gasket and the seeding bottle cap.
By adopting the technical scheme, compared with the prior art, the utility model has the following technical effects:
according to the dual-purpose conical bottle for microbial culture and fed-batch inoculation, the detachable inoculation bottle cap is arranged, and the conical bottle can be used as a culture bottle to culture any microbes when the bottle cap is not installed; when inoculation fermentation is needed, the feeding inoculation can be carried out by installing an inoculation bottle cap, and in the process from the conical bottle to the fermentation tank, the introduction of air flow containing foreign bacteria can be avoided to the greatest extent, so that the pollution of the foreign bacteria during the inoculation can be reduced to the greatest extent; the air guide structure can maintain the air pressure in the conical flask in the feeding inoculation process, so that negative pressure generated in the flask is prevented from influencing the feeding inoculation. The dual-purpose conical flask for microbial culture and fed-batch inoculation provided by the utility model has two functions of microbial culture and fed-batch inoculation, can reduce the possibility of mixed bacteria pollution in the inoculation process, and is suitable for the fields of culture of any microorganisms and fermentation inoculation.
Drawings
FIG. 1 is a schematic diagram of a conical flask in a dual-purpose conical flask for culturing and feeding inoculation of microorganisms according to an embodiment of the present invention;
FIG. 2 is a schematic view of an inoculation bottle cap in a dual-purpose conical bottle for microorganism culture and feeding inoculation provided by an embodiment of the utility model;
FIG. 3 is a schematic diagram of a rubber gasket in a dual-purpose conical flask for culturing and feeding microorganism according to an embodiment of the present invention;
FIG. 4 is a schematic diagram of a metal gasket in a dual-purpose conical flask for culturing and feeding microorganism according to an embodiment of the present invention;
FIG. 5 is a schematic diagram of an assembled together of a conical flask, an inoculation bottle cap, a rubber gasket and a metal gasket in a dual-purpose conical flask for microbial cultivation and feeding inoculation provided by an embodiment of the utility model;
wherein the reference numerals are: 10-conical bottle, 11-bottle mouth, 12-bottle body, 20-inoculating bottle cap, 30-inoculating tube, 31-first metal tube, 32-first rubber hose, 33-second rubber hose, 34-metal needle, 40-air guide structure, 41-second metal tube, 42-third rubber hose, 43-degerming component, 50-rubber gasket and 60-metal gasket.
Detailed Description
The utility model provides a microorganism culture and feeding inoculation dual-purpose conical flask, and the technical scheme of the utility model is further described in detail in the following with the accompanying drawings so as to better understand the utility model, but the utility model is not limited in scope.
As shown in figures 1-4, the utility model provides a microorganism culture and feeding inoculation dual-purpose conical flask, which comprises a conical flask 10, an inoculation bottle cap 20, a inoculation pipe 30 and an air guide structure 40. The conical flask 10 comprises a conical flask body 12 and a cylindrical bottle mouth 11 and is used as a common conical flask for carrying out microorganism culture; the inoculation bottle cap 20 is detachably arranged on the bottle mouth 11; and may be secured to the mouth 11 by a connecting structure and be formed as a unitary structure with the erlenmeyer flask 10.
The inoculation pipe 30 is arranged on the inoculation bottle cap 20, penetrates through the inoculation bottle cap 20, one end of the inoculation pipe is positioned in the bottle body 12, and the other end of the inoculation pipe is connected with a feeding inoculation device for feeding inoculation; the air guide structure 40 is disposed on the inoculation bottle cap 20, penetrates the inoculation bottle cap 20, has one end located in the bottle body 12 and the other end located outside, and is used for maintaining the air pressure in the erlenmeyer flask 10.
In a preferred embodiment of the utility model, the connection comprises an external thread and a matching internal thread. The external thread is arranged on the outer surface of the bottle mouth 11; the internal threads are provided on the inner surface of the sidewall of the inoculation bottle cap 20. According to the utility model, the bottle mouth 11 and the inoculation bottle cap 20 are provided with matched threads, so that the inoculation bottle cap 20 and the conical flask 10 can be connected or detached, and when the inoculation bottle cap 20 is not installed, the conical flask 10 can be used as a common conical flask for culturing strains; when the inoculation fermentation is needed, the inoculation bottle cap 20 can be installed, and the feeding inoculation is carried out through the inoculation pipe 30 and the air guide structure 40 on the inoculation bottle cap.
The seed tube 30 includes a first metal tube 31, a first rubber hose 32, and a second rubber hose 33. The first metal tube 31 is arranged on the inoculation bottle cap 20 and penetrates through the inoculation bottle cap 20; the first rubber hose 32 is arranged at the lower end of the first metal tube 31, is positioned in the bottle body 12 and is close to the bottom of the bottle body 12; the second rubber hose 33 is arranged at the upper end of the first metal pipe 31 and is connected with a feeding inoculation device.
The end of the second rubber hose 33 remote from the first metal tube 31 is also provided with a metal needle 34.
The air guide structure 40 includes a second metal pipe 41, a third rubber hose 42, and a sterilizing member 43. The second metal tube 41 is arranged on the inoculation bottle cap 20, penetrates through the inoculation bottle cap 20 and extends into the bottle body 12 from the lower end; the third rubber hose 42 is arranged at the upper end of the second metal pipe 41 and communicated with the outside; the sterilizing component 43 is disposed on the third rubber hose 42 to prevent external bacteria from entering the bottle body 12. In a preferred embodiment of the present invention, the sterilizing member 43 is a sterilizing filter.
The dual-purpose erlenmeyer flask further comprises a rubber gasket 50 detachably arranged in the inoculation bottle cap 20; the rubber gasket 50 is provided with two through holes for the seed tube 30 and the air guide 40 to pass through.
The dual-purpose erlenmeyer flask further comprises a metal gasket 60 detachably disposed in the inoculation bottle cap 20; the metal shim 60 is provided with two through holes for the seed tube 30 and the air guide 40 to pass through.
When rubber gasket 50 and metal gasket 60 are installed in the inoculation bottle cap 20, rubber gasket 50 is located between metal gasket 60 and inoculation bottle cap 20. The rubber gasket 50 and the metal gasket 60 can ensure the sealing between the inoculation bottle cap 20 and the bottle opening 11, and prevent external gas from entering the conical bottle 10.
When the dual-purpose conical flask for microbial culture and fed-batch inoculation is used for strain culture, the inoculation bottle cap 20 is not needed, and only sterile gauze or a sealing film with a breather valve is wrapped at the opening of the conical flask 10, so that the conical flask can be used as a common conical flask for culture.
As shown in fig. 5, when the dual-purpose erlenmeyer flask for microorganism culture and feeding inoculation provided by the utility model is used for feeding inoculation, the erlenmeyer flask 10 and the inoculation bottle cap 20 need to be connected and installed firstly, and the specific steps are as follows: firstly, sequentially filling a rubber gasket 50 and a metal gasket 60 into the inoculation bottle cap 20; then, performing high-temperature sterilization on the combined inoculation bottle cap 20, the rubber gasket 50 and the metal gasket 60; after cooling, the inoculating bottle cap 20 and the conical flask 10 are tightly screwed and fixed together, and the metal needle 34 is tightly wrapped by sterile gauze to avoid pollution.
In order to ensure that the whole body can be disinfected at high temperature, the whole body of the dual-purpose conical bottle provided by the utility model is made of high-temperature resistant materials.
The dual-purpose conical flask provided by the utility model is used for inoculation, and comprises the following steps: firstly, transferring an assembled conical flask with seed liquid to a side of a fermentation tank to be inoculated after sterilization; then, taking down a knob nut at the feeding port of the fermentation tank, leaking out the rubber plug, taking down the sterile gauze on the metal needle 34 and quickly inserting the sterile gauze into the rubber plug of the fermentation tank; and pumping the seed liquid in the conical flask into the fermentation tank by using a peristaltic pump in a feeding manner to complete inoculation, and maintaining positive pressure in the fermentation tank in the feeding process.
The air guide structure 40 can maintain the air pressure in the conical flask 10 in the feeding process, so that the situation that seed liquid is difficult to pump into the fermentation tank due to negative pressure is avoided, and the sterilizing part 43 can maintain the air pressure in the conical flask 10 and simultaneously avoid the situation that external bacteria enter the conical flask 10 to cause seed liquid pollution.
By using the improved dual-purpose bottle for culturing and inoculating provided by the utility model, the exposure of seed liquid in an external environment can be reduced to the maximum extent in the whole process from the seed culture in the triangular flask to the entering of a fermentation tank, and the air flow containing the foreign bacteria is not introduced in the inoculation process, so that the pollution of the foreign bacteria can be reduced to the maximum extent.
The method for using the dual-purpose bottle for feeding inoculation is verified by a laboratory for many times, and the effective rate reaches 100 percent. For example, in the environment with more infectious microbes, multiple strains such as clostridium butyricum, streptococcus thermophilus and the like are cultured, and fed-batch inoculation and fire circle inoculation contrast tests are carried out, the fire circle inoculation can cause infectious microbe pollution, but the dual-purpose bottle provided by the utility model has no infectious microbe pollution when being used for fed-batch inoculation.
The embodiments of the present invention have been described in detail, but the embodiments are merely examples, and the present invention is not limited to the embodiments described above. Any equivalent modifications or alterations to this practice will occur to those skilled in the art and are intended to be within the scope of this invention. Accordingly, equivalent changes and modifications made without departing from the spirit and scope of the present invention should be covered by the present invention.
Claims (9)
1. A dual-purpose erlenmeyer flask for microbial cultivation and feeding inoculation is characterized by comprising:
the conical flask (10) comprises a conical flask body (12) and a cylindrical bottle mouth (11) and is used for carrying out microorganism culture;
the inoculation bottle cap (20) is detachably arranged on the bottle mouth (11); can be fixed on the bottle mouth (11) through a connecting structure and forms an integral structure with the conical bottle (10);
the inoculation pipe (30) is arranged on the inoculation bottle cap (20), penetrates through the inoculation bottle cap (20), one end of the inoculation pipe is positioned in the bottle body (12), and the other end of the inoculation pipe is connected with a feeding inoculation device for feeding inoculation; and
the air guide structure (40) is arranged on the inoculation bottle cap (20), penetrates through the inoculation bottle cap (20), one end of the air guide structure is positioned in the bottle body (12), and the other end of the air guide structure is positioned outside and used for maintaining the air pressure in the conical bottle (10).
2. The dual-purpose erlenmeyer flask for microbial culture and fed-batch inoculation of claim 1, wherein the connecting structure comprises:
the external thread is arranged on the outer surface of the bottle mouth (11); and
the internal thread is arranged on the inner surface of the side wall of the inoculation bottle cap (20) and matched with the external thread.
3. The dual-purpose erlenmeyer flask for microbial culture, fed-batch inoculation of claim 1, wherein the inoculation tube (30) comprises:
a first metal tube (31) arranged on the inoculation bottle cap (20) and penetrating through the inoculation bottle cap (20);
the first rubber hose (32) is arranged at the lower end of the first metal pipe (31) and is positioned in the bottle body (12); and
and the second rubber hose (33) is arranged at the upper end of the first metal pipe (31) and is connected with the feeding inoculation equipment.
4. The dual-purpose erlenmeyer flask for microbiological culture, feeding and inoculation as claimed in claim 3, wherein the end of the second rubber hose (33) far from the first metal tube (31) is further provided with a metal needle (34).
5. The dual-purpose erlenmeyer flask for microbial culture, fed-batch inoculation of claim 1 wherein the gas directing structure (40) comprises:
the second metal pipe (41) is arranged on the inoculation bottle cap (20), penetrates through the inoculation bottle cap (20), and extends into the bottle body (12) from the lower end;
a third rubber hose (42) which is provided at the upper end of the second metal pipe (41) and is communicated with the outside; and
and the sterilizing component (43) is arranged on the third rubber hose (42) and is used for preventing external bacteria from entering the bottle body (12).
6. The dual-purpose erlenmeyer flask for microbial cultivation, fed-batch inoculation as claimed in claim 5, characterized in that said sterilization means (43) is a sterilization filter.
7. The dual-purpose erlenmeyer flask for microbiological culture and feeding and inoculation as claimed in claim 1, further comprising a rubber gasket (50) removably mounted in said inoculation bottle cap (20); two through holes are formed in the rubber gasket (50) and used for enabling the inoculation tube (30) and the air guide structure (40) to penetrate through.
8. The dual-purpose erlenmeyer flask for microbiological culture and feeding and inoculation as claimed in claim 7, further comprising a metal gasket (60) removably mounted in said inoculation bottle cap (20); two through holes are formed in the metal gasket (60) so that the seed receiving pipe (30) and the air guide structure (40) can penetrate through the through holes.
9. The dual-purpose erlenmeyer flask for microbial culture and feeding and inoculation as claimed in claim 8, wherein the rubber gasket (50) is located between the metal gasket (60) and the inoculation bottle cap (20).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121194740.5U CN215480907U (en) | 2021-05-31 | 2021-05-31 | Dual-purpose erlenmeyer flask of microorganism culture, fed-batch inoculation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121194740.5U CN215480907U (en) | 2021-05-31 | 2021-05-31 | Dual-purpose erlenmeyer flask of microorganism culture, fed-batch inoculation |
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| Publication Number | Publication Date |
|---|---|
| CN215480907U true CN215480907U (en) | 2022-01-11 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202121194740.5U Active CN215480907U (en) | 2021-05-31 | 2021-05-31 | Dual-purpose erlenmeyer flask of microorganism culture, fed-batch inoculation |
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|---|---|
| CN (1) | CN215480907U (en) |
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2021
- 2021-05-31 CN CN202121194740.5U patent/CN215480907U/en active Active
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