CN212915721U - A centrifuging tube for preparing PRP - Google Patents
A centrifuging tube for preparing PRP Download PDFInfo
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- CN212915721U CN212915721U CN202020993102.9U CN202020993102U CN212915721U CN 212915721 U CN212915721 U CN 212915721U CN 202020993102 U CN202020993102 U CN 202020993102U CN 212915721 U CN212915721 U CN 212915721U
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Abstract
The utility model provides a centrifuging tube for preparing PRP, whole blood is through dividing into plasma above, the platelet leucoderma layer in the middle of and the red blood cell below after once centrifugation, extrudes the hose bottom gently and pushes the leucoderma layer into hard tube section, assembles inner tube and outer tube again after closing the valve member, can carry out centrifugation for the second time, need not to shift, easy operation, very big simplification preparation technology; meanwhile, the centrifugal tube can be used for preparing PRP by a conventional centrifugal machine, a special centrifugal machine is not needed, and the equipment cost is reduced.
Description
Technical Field
The utility model relates to the field of medical equipment, specific theory has related to a centrifuging tube for preparing PRP.
Background
Platelet Rich Plasma (PRP) technology has emerged in the 90 s of the 20 th century, and since Whitman et al, 1997, first applied Platelet Rich Gel (PRG) topically to the study of treating wounds, PRG has gradually become a research hotspot in the field of tissue engineering, particularly in the repair of bone wound defects, and has gained some degree of clinical use.
PRP is a high-concentration platelet-containing plasma obtained by subjecting fresh whole blood to a specific centrifugation method, and is a platelet concentrate. The platelet rich plasma gel is a jelly-like substance formed by adding an activator into the platelet rich plasma, and in the process of activating the platelet rich plasma to form the gel, the platelets release various cell growth factors such as vascular endothelial growth factor, platelet derived growth factor, insulin-like growth factor, transforming growth factor beta 1, transforming growth factor beta 2 and the like through degranulation.
The basic principle of PRP preparation is to separate PRP from whole blood by density gradient centrifugation based on the difference in sedimentation coefficient of each constituent component in blood. The common preparation method comprises the following steps:
the Anitua method: centrifuging for 1 time at 160 Xg force for 6 min, separating whole blood in the tube into 3 layers, the upper layer is supernatant, the lower layer is red blood cells, and a thin pale yellow layer is visible at the junction of the two layers, i.e. platelet-rich plasma layer. Sucking the supernatant to 3mm above the interface, discarding, sucking the whole supernatant to about 3mm (6 mm +) below the interface, and shaking gently to obtain platelet-rich plasma with average platelet concentration of about 433000/mm3。
Sonnleitner method: centrifuging for 2 times, centrifuging at 160 × g for 20 min for 1 time, sucking all supernatant, sucking components above 6 mm of thin platelet layer and interface, transferring to another centrifugal tube, centrifuging at 400 × g for 15 min, removing components on interface, and mixing the rest components to obtain platelet-rich plasma. The platelet concentration of the blood vessel is even more than 2000000/mm3Up to approximately 10 times the normal platelet concentration.
Petrungaro method: and (3) centrifuging for 2 times, absorbing all supernatant liquid to 3mm below the interface after centrifuging for 6 min at 1500 Xg for the 1 st time, transferring to another centrifuge tube, balancing, and centrifuging for 6 min at 1000 Xg again. The liquid in the centrifugal tube is divided into two layers, the supernatant of the upper layer is platelet poor plasma, and the lower layer is platelet concentrate. Sucking about 3/4 supernatant fluid and discarding, shaking the rest components to obtain the platelet rich plasma.
Landersberg method: centrifuging at 200 Xg for 10 min, sucking all supernatant to 3mm below the interface, transferring to another centrifuge tube, balancing, and centrifuging at 200 Xg for 10 min; the liquid in the centrifuge tube is divided into 2 layers, the supernatant is platelet poor plasma, the lower layer is platelet concentrate, about 3/4 supernatant is absorbed and discarded, and the rest isShaking and mixing to obtain the platelet-rich plasma. Landersberg believes that centrifugal forces in excess of 250 Xg are likely to cause excessive platelet damage, and platelet-rich plasma platelet levels obtained by this method can reach 687000/mm3。
The Aghaloo method: the transfer method is the same as Landersberg method, and includes centrifuging at force of 215 × g for 10 min for 1 time and centrifuging at force of 863 × g for 10 min for 2 times to obtain platelet-rich plasma with platelet concentration of 1050000/mm3。
Clinical verification shows that the platelet concentration, the activation rate and the growth factor concentration of PRP prepared by the centrifugation condition of the Landersberg method are relatively optimal.
From the above, the concentration of platelets prepared by the primary centrifugation method is low, while the secondary centrifugation method requires manual operation of a transfer centrifuge tube, and the requirement on aseptic conditions is high. Thus, various sets of PRP preparation systems are available, 9 of which are approved and commercially produced by the FDA in the United states, wherein some of the PRP preparation systems can rapidly and stably obtain PRP, but the obtained PRP has large difference in platelet recovery rate and concentration, such as only 31% + -15% in the Sequire system, and the PRP platelet concentration prepared by the AutoloGel and the Sequire systems is 1 time and 1.6 times of the physiological concentration respectively, and does not reach the therapeutic concentration at which the PRP plays a role. In addition, these PRP production systems are expensive and not conducive to the wide clinical application of PRP technology. Domestic PRP preparation is mostly applied to PRP centrifugal sets imported from Korea, the price is high, a special centrifugal machine is needed, and the prepared PRP is low in concentration and complex to operate. The price of PRP centrifugal suit produced in China is also 7000-8000 yuan, which is not favorable for clinical popularization and especially not suitable for the treatment and application of chronic wounds needing multiple applications.
Most of centrifugal tubes for preparing PRP by secondary centrifugation in the current market are provided with a spin lock cap in an erythrocyte cavity of the centrifugal tube, the spin lock cap in the erythrocyte cavity is rotated after primary centrifugation to push a platelet leucocyte layer upwards, then a connecting port between the locked erythrocyte cavity and a PPP cavity is blocked by an erythrocyte lock rod, and the centrifugal tube is centrifuged again after being inverted, but the centrifugal tube has high cost, difficult locking process and easy leakage; in addition, when the red blood cells are few, the white membrane layer cannot be pushed away from the red blood cell cavity after the locking cap is screwed to the bottom; meanwhile, a special centrifuge is required, and the equipment cost is high.
In order to solve the above problems, people are always seeking an ideal technical solution.
Disclosure of Invention
The utility model aims at the not enough of prior art to a centrifuging tube for preparing PRP is provided.
In order to realize the purpose, the utility model discloses the technical scheme who adopts is:
the utility model provides a centrifuging tube for preparing PRP, include the stereoplasm outer tube, set up in the inside inner tube of stereoplasm outer tube, the uncovered top cap that sets up in top of stereoplasm outer tube, and stereoplasm outer tube bottom seals, the inner tube is the multistage structure, including the hard pipeline section that is located upper portion, the hose section that is located the below, the middle part connecting piece of connecting hard pipeline section and hose section, hose section bottom seals, the middle part connecting piece includes the connecting pipe and is used for adjusting the valve member of intercommunication about the inner tube.
Based on the above, the outer diameter of the inner pipe is adapted to the inner diameter of the hard outer pipe, the connecting pipe is also a hose, and the valve assembly is a clamping piece arranged corresponding to the connecting pipe.
Based on the above, the hard outer pipe and the top cover are in threaded connection.
Based on the above, the height of inner tube with the stereoplasm outer tube suits, the inside correspondence of top cap the top of hard tube section sets up the flexible glue, the top cap outside corresponds the flexible glue sets up dustproof fungi-proofing enclosed membrane.
Based on the above, the top surface of the top cover is a plane, the bottom of the hard outer pipe is a spherical surface, and the shape of the hard outer pipe is matched with that of the centrifugal sleeve of the centrifugal machine.
Based on the above, the outer diameters of the hard pipe section and the soft pipe section are the same, the outer diameter of the connecting pipe is smaller than that of the hard pipe section, and transition parts are arranged on the lower part of the hard pipe section and the upper part of the soft pipe section.
Based on the above, the middle connecting piece is a two-way luer connector, and the middle connecting piece is in interference fit with the hose section.
Compared with the prior art, the utility model has substantive characteristics and progress, in particular to the utility model provides a centrifuge tube for preparing PRP, after one-time centrifugation, whole blood is divided into plasma above, platelet leucocyte layer in the middle and red blood cells below, the bottom of the soft tube is slightly squeezed to push the leucocyte layer into the hard tube section, after the valve component is closed and the inner tube and the outer tube are reassembled, the second centrifugation can be carried out, the transfer is not needed, the operation is simple, and the preparation process is greatly simplified; meanwhile, the centrifugal tube can be used for preparing PRP by a conventional centrifugal machine, a special centrifugal machine is not needed, and the equipment cost is reduced.
Drawings
Fig. 1 is a schematic structural diagram of the present invention.
In the figure: 1. a hard outer tube; 2. a hard pipe section; 3. a hose section; 4. a connecting pipe; 5. a valve assembly; 6. a top cover; 7. a sealing film; 8. and (4) soft glue.
Detailed Description
The technical solution of the present invention will be described in further detail through the following embodiments.
As shown in fig. 1, a centrifuging tube for preparing PRP, include stereoplasm outer tube 1, set up in the inside inner tube of stereoplasm outer tube 1, the uncovered top that sets up of stereoplasm outer tube 1 seals bottom, the inner tube is the multistage structure, including the hard pipe section 2 that is located the upper portion, the hose section 3 that is located the below, the middle part connecting piece of connecting hard pipe section 2 and hose section 3, the 3 bottom of hose section are sealed, the middle part connecting piece includes connecting pipe 4 and is used for adjusting the valve member 5 of intercommunication about the inner tube.
Specifically, the hard pipe section is a PPP pipe, and the soft pipe section is a erythrocyte pipe. In addition, the specification of the centrifuge tube can be designed to be 5\10\20\50 mL, and the ratio of blood cells to other components is about 1:4 according to experimental data, but the upper hard tube section can be properly enlarged by 2 mL for safety. Such as: 5mL of centrifuge tube with the specification, wherein the blood drawing amount is 5mL, but the total volume of the tube is 7 mL, the proportion is 4mL of a lower hose and 3 mL of an upper hard tube; 10 mL centrifuge tube, the volume of blood drawn is 10 mL, but the total tube volume is 12 mL, the ratio is 8 mL for the lower tube and 4mL for the upper tube.
The whole blood is divided into plasma above, a platelet leucocyte layer in the middle and red blood cells below after being centrifuged, the leucocyte layer is pushed into the hard tube section by slightly squeezing the bottom of the hose, the inner tube and the outer tube are reassembled after the valve assembly is closed, secondary centrifugation can be carried out without transferring, the operation is simple, and the preparation process is greatly simplified; in addition, the serum in the upper hard tube section can also be directly extracted.
Further, the outer diameter of the inner pipe is adapted to the inner diameter of the hard outer pipe 1, the connecting pipe 4 is also a hose, and the valve assembly 5 is a clamping member arranged corresponding to the connecting pipe 4.
Specifically, the hard outer tube 1 and the top cover 6 are in threaded connection.
Further, the height of the inner pipe is matched with that of the hard outer pipe 1, soft rubber 8 is arranged inside the top cover 6 and corresponds to the top of the hard pipe section 2, and a dustproof and antibacterial sealing film 7 is arranged outside the top cover 6 and corresponds to the soft rubber 8. The inner tube is located inside the outer tube in the use, and its upper end and lower extreme and outer tube bottom and top cap butt press from both sides tight fixed inner tube, have further strengthened the rigidity of centrifuging tube, make the inner tube not fragile when the centrifugation. Specifically, the outer tube and the inner tube are assembled into a whole in an initial state, the sealing film is uncovered, blood is injected through the soft glue, the centrifugation is carried out for the first time, and blood cells are deposited in the red blood cell hose section below the centrifugation. Taking out the centrifuge tube, rotating to remove the external hard tube, slightly extruding the red blood cell hose, allowing the platelet white membrane on the upper layer of the centrifuge tube to enter the PPP tube through the middle connecting tube, clamping the lock catch, then screwing the external hard tube again, and centrifuging for the second time. Pumping out 3/4 above PPP tube, discarding the rest, and shaking up to obtain PRP.
Furthermore, the top surface of the top cover 6 is a plane, the bottom of the hard outer tube 1 is a spherical surface, the shape of the hard outer tube is matched with that of a centrifugal sleeve of a centrifugal machine, the PRP can be prepared by adopting a conventional centrifugal machine, a special centrifugal machine is not needed, and the equipment cost is reduced.
Further, the outer diameters of the hard pipe section 2 and the soft pipe section 3 are the same, the outer diameter of the connecting pipe 4 is smaller than that of the hard pipe section 2, and transition portions are arranged on the lower portion of the hard pipe section 2 and the upper portion of the soft pipe section 3.
Further, the middle connecting piece is a two-way luer connector, and the middle connecting piece is in interference fit with the hose section 3.
Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention and not to limit it; although the present invention has been described in detail with reference to preferred embodiments, it should be understood by those skilled in the art that: the invention can be modified or equivalent substituted for some technical features; without departing from the spirit of the present invention, it should be understood that the scope of the claims is intended to cover all such modifications and variations.
Claims (7)
1. A centrifuging tube for preparing PRP, its characterized in that: including the stereoplasm outer tube, set up in the inside inner tube of stereoplasm outer tube, the uncovered top cap that sets up in top of stereoplasm outer tube, and stereoplasm outer tube bottom seals, the inner tube is the multistage structure, including the hard pipeline section that is located upper portion, the hose section that is located the below, the middle part connecting piece of connecting hard pipeline section and hose section, the hose section bottom seals, the middle part connecting piece includes the connecting pipe and is used for adjusting the valve member of intercommunication about the inner tube.
2. The centrifuge tube for preparing PRP according to claim 1, wherein: the outer diameter of the inner pipe is matched with the inner diameter of the hard outer pipe, the connecting pipe is also a hose, and the valve component is a clamping piece arranged corresponding to the connecting pipe.
3. The centrifuge tube for preparing PRP according to claim 1 or 2, wherein: the hard outer pipe is in threaded connection with the top cover.
4. The centrifuge tube for preparing PRP according to claim 3, wherein: the height of the inner pipe is matched with that of the hard outer pipe, soft rubber is arranged at the top of the top cover corresponding to the hard pipe section, and the outer portion of the top cover corresponds to the soft rubber and is provided with a dustproof and antibacterial sealing film.
5. The centrifuge tube for preparing PRP according to claim 4, wherein: the top surface of the top cover is a plane, the bottom of the hard outer pipe is a spherical surface, and the shape of the hard outer pipe is matched with that of a centrifugal sleeve of a centrifugal machine.
6. The centrifuge tube for preparing PRP according to claim 1, wherein: the outer diameters of the hard pipe section and the soft pipe section are the same, the outer diameter of the connecting pipe is smaller than that of the hard pipe section, and transition portions are arranged on the lower portion of the hard pipe section and the upper portion of the soft pipe section.
7. The centrifuge tube for preparing PRP according to claim 6, wherein: the middle connecting piece is a two-way luer connector and is in interference fit with the hose section.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN202020993102.9U CN212915721U (en) | 2020-06-03 | 2020-06-03 | A centrifuging tube for preparing PRP |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
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CN202020993102.9U CN212915721U (en) | 2020-06-03 | 2020-06-03 | A centrifuging tube for preparing PRP |
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CN212915721U true CN212915721U (en) | 2021-04-09 |
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CN202020993102.9U Expired - Fee Related CN212915721U (en) | 2020-06-03 | 2020-06-03 | A centrifuging tube for preparing PRP |
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CN (1) | CN212915721U (en) |
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2020
- 2020-06-03 CN CN202020993102.9U patent/CN212915721U/en not_active Expired - Fee Related
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CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20210409 |
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CF01 | Termination of patent right due to non-payment of annual fee |