CN211972357U - Special culture apparatus for immune cell culture - Google Patents

Abstract

The utility model relates to a special culture device special for immune cell culture, which comprises a culture bottle and a culture bottle support, wherein the culture bottle is detachably arranged on the culture bottle support, the culture bottle comprises a bottle body, three bottle openings are uniformly arranged on one side of the bottle body at intervals, a bottle cap is arranged on the bottle opening in a uniform threaded manner, a drainage port is further formed on one side surface of the bottle body, the drainage port is connected with a drainage tube in a threaded manner, an adjusting valve is further arranged on the drainage tube, and end shafts are further symmetrically and fixedly arranged at two ends of the bottle body; the culture bottle support comprises a base and a bottle frame, wherein a groove is formed in the middle of the upper surface of the base, a side groove is formed in the base on one side of the groove, a platform is formed in the base on the other side of the groove, and the bottle frame is symmetrically arranged on two sides of the platform. The utility model discloses be convenient for external amplification culture operation, can observe the cell state, be convenient for the cell blows and beats, can in time blow away the cell group, improve cell survival rate and cell quantity.

Description

Special culture apparatus for immune cell culture

Technical Field

The utility model belongs to the technical field of immune cell culture, a culture flask technique is related to, especially a special culture apparatus who is exclusively used in immune cell culture.

Background

Biological cell immunotherapy is a popular medical research direction at present, and is characterized in that peripheral blood of a patient or a corresponding donor is extracted to separate immune cells of the patient or the corresponding donor in a biotechnology engineering mode, the patient or the corresponding donor is modified to have a certain function of killing tumor cells, and then the modified cells with aggressiveness are returned to the body of the patient, so that the purpose of treating tumors is achieved, and the cells which are commonly used are T cells, CAR-T cells, NK cells and CIK cells. The number of immune cells separated from a normal human body is rare, and the treatment effect cannot be achieved, so the immune cells separated from the human body need to be induced, modified and amplified in vitro, the general immune cells need to be amplified by more than 200 times when reaching the treatment effect, the immune cells are suspension cells and are amplified by 200 times in vitro, the volume of a cell culture medium is large, researchers carry out the experiment of the amplification of the immune cells in vitro by using two experimental consumables at present, one is a culture bag, the culture bag has the advantages of being capable of containing a large-volume culture medium, being simple and convenient to operate, but the culture bag cannot blow the immune cells in time, the normal immune cells grow in a cohesive mass mode, if the cell mass is not blown away in time every day, the survival rate of the immune cells and the growth speed of the immune cells are influenced, the culture bag cannot clearly observe the cell state under a microscope, and the culture bag cannot uniformly mix the cells during cell counting, causing the defects of inaccurate counting and the like. The second kind is the culture bottle, the biggest cell culture bottle volume in the market at present is 225m2, generally 175m2 is the most commonly used, it needs a plurality of culture bottles to cultivate with a small-volume culture bottle a sample, immune cells need blow off the cell every day and every 2 days cell number of calculation adjust cell density, divide a cell into a plurality of culture bottles, the cell growth speed in each culture bottle is different, the state is different, so count and need count every bottle, when adjusting the cell proportion, each bottle all needs individual regulation, the operation process is very loaded down with trivial details.

The following patent documents relevant to the present application are found by search, and specific disclosures are as follows:

1. a continuous pipettor (CN207287472U) comprises a standard pipetting handle and a standard pipetting gun head matched with the standard pipetting handle, wherein the standard pipetting handle is matched and connected with a collection container through a connecting pipe, and the collection container is matched and connected with an air suction pump through an air suction pipe; the sealing plug is arranged on the bottle mouth of the collecting container in a matching mode, the filtering plug is arranged on the air exhaust pipe, the connecting pipe is a hose, and the air exhaust pump is a roller type negative pressure air exhaust pump.

2. The continuous liquid transfer device (CN203591816U) for the liquid transfer device comprises a liquid transfer gun, one end of the liquid transfer gun is provided with a snap fastener, the other end of the liquid transfer gun is provided with a gun head interface, the continuous liquid transfer device comprises a gun head, the gun head is matched and connected with the gun head interface in a use state, a mother liquid container filled with mother liquid in a sealing state is formed on the gun head, the mother liquid container is provided with a mother liquid leading-out connecting pipe used for leading the mother liquid in a mother liquid container cavity of the mother liquid container into a gun head cavity of the gun head for leading out a gun head liquid outlet hole of the gun head, and the mother liquid leading-out connecting pipe is integrally connected with the gun head and is communicated with the gun head.

3. The utility model provides an electronic continuous pipetting gun of hand-held type (CN206951232U), the electronic continuous pipetting gun of hand-held type adopts the modularization integration, and wherein all control unit all can adopt conventional module on the market to replace, and it is convenient to maintain, the stable performance, low cost, and the multiple liquid measure of moving is adjusted to the simple switch of accessible, moves the accurate reliable of liquid measure.

Through the contrast of technical characteristics, above-mentioned patent literature of disclosing with the utility model discloses a technical structure is inequality, can not influence the creativity and the novelty of the utility model application.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to overcome prior art's weak point, provide one kind and be convenient for external amplification culture operation, can observe the cell state, be convenient for the cell blows and beats, can in time blow the cell group open, improve the special culture apparatus that immune cell cultivates of being exclusively used in of cell survival rate and cell quantity.

The utility model provides a its technical problem take following technical scheme to realize:

a special culture device special for immune cell culture comprises a culture bottle and a culture bottle support, wherein the culture bottle is detachably mounted on the culture bottle support and comprises a bottle body, three bottle openings are uniformly and alternately formed in one side of the bottle body, the bottle openings are higher than the bottle body, breathable bottle caps are mounted at the bottle openings in a threaded manner, a drainage opening is further formed in one side face of the bottle body, one end of the drainage opening is further connected with a drainage tube in a threaded manner, the drainage tube is further connected with an adjusting valve capable of adjusting flow, and end shafts for supporting and hanging are further symmetrically and fixedly mounted at two ends of the bottle body; the blake bottle holds in the palm and includes base and bottleholder, a base upper surface middle part system has a recess, the base upper surface of recess one side still a system has a side groove, a system has a platform on the base upper surface of recess opposite side, platform bilateral symmetry demountable installation has the bottleholder, the bottleholder includes the bracing piece and inserts the bifurcation, insert an integrative system in the bracing piece top of bifurcation for support the end axle at blake bottle both ends.

Moreover, the drainage tube is made of plastic.

And the upper surface of the bottle body is symmetrically and detachably provided with a handle through buttons.

Moreover, the handle is made of latex.

Moreover, the lower part in the base is provided with a solid bearing block.

Also, the side grooves are inclined at 45 ° to the base surface.

The utility model has the advantages that:

the utility model discloses simple structure, modern design, the design of blake bottle makes the storage that the culture medium can be better, and the cell is convenient for observe when the immune cell bulky is cultivateed, and the immune cell external amplification cultivates operation process is simple and convenient moreover, can also satisfy the immune cell bulky when cultivateing, and the cell of being convenient for blows and beats, can in time blow the cell group open, improves cell survival rate and cell quantity.

The utility model discloses it is more accurate to utilize the blake bottle to hold in the palm when combining the blake bottle and making the bulky culture of immune cell count to accurate adjustment cell concentration, and during the preparation immune cell preparation, can carry the cell fast and carry out the centrifugation in the centrifuge tube.

Drawings

FIG. 1 is a schematic structural view of the present invention;

FIG. 2 is a schematic view of the structure of the culture flask of the present invention;

FIG. 3 is a schematic view of the structure of the flask holder of the present invention;

FIG. 4 is a state diagram of the insertion side groove of the present invention;

fig. 5 is a state diagram of the utility model hanging on the bottle rack.

Detailed Description

The present invention will be described in further detail with reference to the following embodiments, which are illustrative, not restrictive, and the scope of the invention should not be limited thereto.

A special culture device special for immune cell culture comprises a culture bottle 5 and a culture bottle support 1, wherein the culture bottle is detachably mounted on the culture bottle support and comprises a bottle body, the bottle body is 25 cm long, 20 cm wide and 6 cm high, and can be used as a culture medium with a maximum capacity of 2000ml, when the bottle body is placed on a microscope, cell observation is facilitated, one side of the bottle body inclines upwards, and three bottle openings are uniformly distributed at intervals at the inclined position, so that the bottle openings are higher than the bottle body, the culture medium is prevented from flowing out of the bottle openings, the diameter of each bottle opening is 3 cm, large-volume cell blowing is facilitated, breathable bottle caps 5-6 are mounted at the bottle openings through threads, and cells at all corners in the bottle can be blown;

the utility model discloses in the implementation, because the volume of blake bottle body is great, when the cell preparation was done to the immune cell, need with the cell centrifugation, 2000ml volume culture medium if derive with the mode of absorption and centrifuge, can consume time very much, pour out with the bottleneck moreover, because the bottleneck is opened many times, increase the contamination probability, therefore, a body side still system has a drainage mouth 5-4, drainage mouth is apart from body upper surface 0.5 cm, and the culture medium can not contact the drainage mouth, drainage mouth one end is still threaded connection intercommunication and is provided with drainage tube 5-7, still connect on the drainage tube and be provided with adjustable flow's governing valve 5-5, the drainage tube is the plastic construction that can carry out the sterilization operation.

In the specific implementation of the utility model, because the cell culture bottle can not rock when needing to take the cell from the incubator to observe every time, because once rock, can not see cell state and density accurately under the mirror, the immunocyte is the suspension cell, must wait the cell subside can observe, because the large capacity culture bottle volume is great, liquid is more, weight is great, therefore can dismantle still symmetrically be provided with handle 5-3 through button 5-2 on the body upper surface through the button, the handle is the latex material, can ultraviolet irradiation sterilization, lifts the handle simultaneously, can steadily take up and shift the culture bottle;

in the specific implementation of the utility model, two ends of the body of the culture bottle are symmetrically and fixedly provided with end shafts 5-1.

In the practical implementation of the utility model, the culture bottle support comprises a base and a bottle rack, the lower part in the base is provided with a solid bearing block, so that the base is more stable, the middle part of the upper surface of the base is integrally provided with a groove 7 for vertically placing the culture bottle, one side of the upper surface of the base at one side of the groove is also integrally provided with a side groove 2, the side groove and the surface of the base form an inclination of 30-45 degrees for obliquely inserting the culture bottle, when the culture bottle is obliquely arranged, cells can be blown and regulated, the upper surface of the base at the other side of the groove is integrally provided with a platform 6, the bottle rack is detachably mounted with bilateral symmetry of the platform, the bottle rack comprises a support rod 4 and an insert fork 3, the insert fork is integrally arranged at the top end of the support rod and used for supporting the end shafts at the two ends of the culture bottle, when immune cell preparation is needed, cell suspension needs to be transferred into a centrifugal tube for, put the pivot axle of blake bottle on the fork of bottle rack for the blake bottle hangs the state, thereby makes the drainage mouth down, is connected drainage tube and centrifuging tube, then opens the governing valve on the drainage tube, with the even input of cell suspension in the centrifuging tube.

Although the embodiments of the present invention and the accompanying drawings are disclosed for illustrative purposes, those skilled in the art will appreciate that: various substitutions, changes and modifications are possible without departing from the spirit and scope of the present invention and the appended claims, and therefore, the scope of the present invention is not limited to the disclosure of the embodiments and the accompanying drawings.

Claims (6)

1. A special culture apparatus special for immune cell culture is characterized in that: the culture bottle comprises a culture bottle and a culture bottle support, wherein the culture bottle is detachably arranged on the culture bottle support, the culture bottle comprises a bottle body, three bottle openings are uniformly distributed on one side of the bottle body at intervals, the bottle openings are higher than the bottle body, breathable bottle caps are arranged at the bottle openings in a threaded manner, a drainage opening is further formed in one side face of the bottle body, one end of the drainage opening is further connected with a drainage tube in a threaded manner, the drainage tube is further connected with an adjusting valve capable of adjusting flow, and end shafts for supporting and hanging are further symmetrically and fixedly arranged at two ends of the bottle body; the blake bottle holds in the palm and includes base and bottleholder, a base upper surface middle part system has a recess, the base upper surface of recess one side still a system has a side groove, a system has a platform on the base upper surface of recess opposite side, platform bilateral symmetry demountable installation has the bottleholder, the bottleholder includes the bracing piece and inserts the bifurcation, insert an integrative system in the bracing piece top of bifurcation for support the end axle at blake bottle both ends.

2. The culture apparatus dedicated to immune cell culture as recited in claim 1, wherein: the drainage tube is made of plastic.

3. The culture apparatus dedicated to immune cell culture as recited in claim 1, wherein: the upper surface of the bottle body is symmetrically and detachably provided with a handle through buttons.

4. The culture apparatus dedicated to immune cell culture as recited in claim 3, wherein: the handle is made of latex.

5. The culture apparatus dedicated to immune cell culture as recited in claim 1, wherein: and a solid bearing block is arranged at the inner lower part of the base.

6. The culture apparatus dedicated to immune cell culture as recited in claim 1, wherein: the side grooves are inclined at 45 ° to the base surface.

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