CN207828321U - The automation stem cells hyperplasia system that a kind of mass and individuation have both - Google Patents
The automation stem cells hyperplasia system that a kind of mass and individuation have both Download PDFInfo
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- CN207828321U CN207828321U CN201721476912.1U CN201721476912U CN207828321U CN 207828321 U CN207828321 U CN 207828321U CN 201721476912 U CN201721476912 U CN 201721476912U CN 207828321 U CN207828321 U CN 207828321U
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Abstract
The automation stem cells hyperplasia system that the utility model provides a kind of mass and individuation has both, including fixing module, cell Proliferation module, liquid storage module and abandon liquid module.Wherein cell Proliferation module can used aloned or multiple integrated uses.The system can be used as a kind of new-type bioreactor, can carry out the Multiplying culture of cell in the system, and nutrient solution and growth factor is made to be continuously maintained in level appropriate, while removing metabolic waste in time.The system can carry out the high-efficient culture and proliferation of target cell under the conditions of passive.The system structure design is succinct, compact, and automation is easy to operate, cost reduction and can individuation and mass synchronize used, be suitable for the fast breeding of various stem cell samples, and clinical biochemical treatment and stem cell transplantation can be widely used in.
Description
Technical field
The utility model belongs to technical field of biotechnology, has both in particular to a kind of mass and individuation
Automate stem cells hyperplasia system.
Background technology
Stem cell population and quality are to ensure that the key of regenerative medicine curative effect.Related small-scale expansion of stem cells and divide at present
Change study on regulation report very much, Canadian STEMCELL Tech companies develop kinds of experiments grade stem cell media, but are directed to
Various certain types of stem cells (candidate stem cell of the mescenchymal stem cell of such as adhere-wall culture and the culture that suspends) still lack ratio
Compared with the effective amplification system of accurately scale.The main problem of stem cell culture at present is a lack of suitable stem cell growth and function
The microenvironment of maintenance, such as single-layer or multi-layer planar substrates or spherical microcarrier (the Cytodex series of such as GE companies),
Basic material ingredient is single, far apart with internal stem cell microenvironment.Therefore, to improve the accurate of expansion of stem cells and transplanting
Controllability is needed for different type stem cell (as the most real active mescenchymal stem cell MSC of clinical conversion, Hematopoietic Stem are thin
Born of the same parents HSC, neural stem cell NSC etc.) and its special-purpose, research and development are with the scale amplification body that cell types and purposes are guiding
System and Testing index.
There are many small-scale expansion of stem cells and differentiation study on regulation report at present, have some microcarrier cultures, culture medium
It is studied with examination criteria.But the reparation for the culture of different types of stem cell for tissue, shortcoming function-stable and safety can
The scale expansion of stem cells system leaned on.However, Clinical Institutions carry out stem cell culture amplification still using routine experiment at present
The equipment of room, and manual mode of operation is used always, the pollution of culture medium is easily caused, and manual operations is inaccurate, efficiency
It is low, take time and effort, it is evident that the application for affecting ex vivo expansion of stem cell.At the same time, although developing profit both at home and abroad
With new-type bioreactor (such as perfusion type, rotation arm type).However, these devices be required for being equipped with operating system such as peristaltic pump,
Micro machine etc., it is inconvenient for operation.And operation cost is increased, it is unfavorable for a large amount of clinical application.
Utility model content
The automation stem cells hyperplasia system that the utility model provides a kind of mass and individuation has both, system design
Simply, compact can realize that unpowered and automatic operating, easy to operate, cost reduction are suitable for various cell samples
Fast breeding, and can be widely applied to clinical biochemical treatment and stem cell transplantation.
Technical solution is used by the utility model solves above-mentioned technical problem：What a kind of mass and individuation had both
It automates stem cells hyperplasia system, including fixing module, cell Proliferation module, liquid storage module and abandons liquid module；Wherein cell increases
It includes at least one Cell proliferation device to grow module；The fixing module is for being installed or placed cell Proliferation module, liquid storage mould
Block and abandon liquid module；The liquid storage module includes liquid storage cylinder and breather valve；The cell Proliferation module includes cell culture chamber, carefully
Filling is microsphere supported in born of the same parents' culture chamber, and microsphere supported top arranges that separation layer, the oral area of cell culture chamber are sealed with sealing cover；Institute
It includes abandoning liquid reclaiming chamber to state and abandon liquid module；Between the liquid storage cylinder and cell culture chamber, cell culture chamber and abandon liquid reclaiming chamber it
Between connected by catheter.
Further, the fixing module includes fixing pedestal, and fixing pedestal is equipped with fixing holder, and liquid storage cylinder passes through the
One fixing folder is fixedly mounted on fixing holder, and cell culture chamber is fixedly mounted on by the second fixing folder on fixing holder, is abandoned
Liquid reclaiming chamber is placed on fixing pedestal.The material of the fixing module is selected from metal, high molecular polymer.
Further, cell culture fluid is housed, the amount of cell culture fluid increases for 100-1000ml/ cells in the liquid storage cylinder
Device, the breather valve ensure that cell culture fluid can smoothly flow out liquid storage cylinder, liquid storage to connect ambient atmosphere and liquid storage cylinder
The first catheter is connected by first interface on chamber, the other end of the first catheter connects cell proliferation chamber by second interface,
First catheter is equipped with the first flow regulator.
Further, the volume of Single cell culture chamber is 100ml；The oral area of the cell culture chamber passes through third interface
The second catheter is connected, the second catheter is stretched into across separation layer in microsphere supported, and the other end of the second catheter passes through the 4th
Liquid culture chamber is abandoned in interface connection, and the second catheter is equipped with second flow speed adjuster.
Further, the microsphere supported ball particle size is 100 μm of -2mm, and the microsphere supported material is selected from high score
It is one or more in sub- polymer, hydrogel, ceramics, metal, glass, silica.
It is further, described that abandon the liquid volume that liquid reclaiming chamber can accommodate be 100-1000ml/ Cell proliferation devices.
Further, first catheter and the second catheter material are selected from polyvinyl chloride, PE, PP plastics
In it is one or more.
Further, the liquid storage cylinder, abandon liquid reclaiming chamber and cell culture chamber material and use sterile high molecular polymer
Material, including polydimethylsiloxane, acrylonitrile-butadiene-styrene copolymer, polycarbonate, polyacrylic acid first
Ester PMMA, polyethylene, polypropylene, polymethylpentene, polystyrene and nylon.
Further, ranging from every point of the flow rate of liquid that first flow regulator and second flow speed adjuster are adjusted
Clock 1-3000 μ L.
In some embodiments, the stem cells hyperplasia system occupation mode is vertical use, in the cloth vertically used
In setting, liquid storage module is located at highest order, and cell Proliferation module is located at middle part, abandons liquid recycling module and is located at lower part, in such knot
Under structure, liquid storage mould cell culture fluid in the block can under the effect of gravity flow directly into cell Proliferation module.
In some embodiments, the stem cells hyperplasia system is used by the way of horizontal, in the cloth that level uses
In setting, liquid storage module is located therein side, and cell Proliferation module is located at middle part, abandons liquid recycling module and is located at other side, at this
Under kind arrangement, system needs that a power plant for drawing cell culture fluid from liquid storage module is arranged.
In some embodiments, the fixing module can hold one or more stem cells hyperplasia modules.
In some embodiments, the interface arrived used in the utility model can be the grafting being directly connected with catheter
The circle interface of formula also can be rotatable hickey.
The automation stem cells hyperplasia system that a kind of mass provided by the utility model and individuation have both is for existing
The advantageous effect of technology is：
The automation stem cells hyperplasia system that mass and individuation have both, is life science, medicine and ecsomatics, biology
The important technological platform of medical treatment research.Has the characteristics that simple, inexpensive, automation.The stem cells hyperplasia system is gathered around
There is huge market-oriented foreground.
The automation stem cells hyperplasia system that mass and individuation have both can be realized multiple in a fixing module
The stem cell of quantity is cultivated simultaneously, effectively simplify system design, avoid due to caused by routine culture time difference not
The problems such as accuracy.
In addition, above-mentioned mass and individuation stem cells hyperplasia system are compared with prior art, also have the following advantages that：
(1) passive operating system (also can be active, such as peristaltic pump), easy to operate, cost reduction can be used；
(2) replacement of culture solution can be carried out automatically, and can effectively keep nutrient solution and growth factor to be continuously maintained in
Level appropriate, while metabolic waste can be effectively removed.
(3) can cell inoculation, cultivate, be grown in a closed removable system, avoidable conventional manual changes liquid
Pollution problem caused by possible, while the stem cell for cultivating identical quantity can effectively save culture solution；
(4) microsphere supported quantity and size can be changed to obtain corresponding stem cell number, accuracy is high.
(5) the automation stem cells hyperplasia system structure design that mass and individuation have both is succinct, compact, convenient
Mobile and transport, and do not need professional's operation, easy to operate, cost reduction.
(6) the utility model patent mass and individuation are taken into account, and new facility and existing instrument are taken into account, can be to stem cell
Collective's feed flow is carried out, collective collects, greatly reduces stem cell toxigenic capacity, can effectively improve stem cell pick-up rate.
In conclusion the utility model in view of the problems of the existing technology, establishes a kind of mass and individuation is simultaneous
Standby automation stem cells hyperplasia system.The system has preferable application in clinical biochemical treatment and stem cell transplantation etc.
Foreground.
Description of the drawings
It should be understood that the following drawings illustrates only some embodiments of the utility model, therefore it is not construed as
Restriction to range for those of ordinary skill in the art without creative efforts, can also basis
These attached drawings obtain other relevant attached drawings.
Fig. 1 provides a kind of structural schematic diagram of the automation stem cells hyperplasia system of individuation.
Fig. 2 is the schematic diagram of the cell Proliferation module of the utility model.
Fig. 3 provides a kind of structural schematic diagram (being vertically arranged) of the automation stem cells hyperplasia system of mass.
Fig. 4 provides a kind of structural schematic diagram (lateral arrangement) of the automation stem cells hyperplasia system of mass.
Drawing reference numeral explanation：
Specific implementation mode
The embodiments of the present invention are described below in detail, the examples of the embodiments are shown in the accompanying drawings, and from beginning
To eventually using the same or similar label same or similar element of expression or element with the same or similar functions.Lead to below
It crosses the embodiment being described with reference to the drawings to be exemplary, it is intended to for explaining the utility model, and should not be understood as to this practicality
Novel limitation.
The embodiments of the present invention are described below in detail in the utility model in order to better understand, the embodiment
Example is shown in the accompanying drawings, in which the same or similar labels are throughly indicated same or similar element or with identical or
The element of similar functions.The embodiments described below with reference to the accompanying drawings are exemplary, it is intended to for explaining the utility model,
And it should not be understood as limiting the present invention.
In the present invention, cell culture chamber 1-6-1, liquid storage cylinder 1-2-1 and material used in liquid reclaiming chamber 1-7-1 is abandoned
Matter can be polystyrene, but be not limited to polystyrene, can also be PDMS, can be also glass, can also be PMMA or other biological
Compatible polymer polymer material is this patent institute protection domain.
In the present invention unless specifically defined or limited otherwise, the terms such as term " connected ", " connection " should be done extensively
Reason and good sense solution may be a detachable connection for example, it may be being fixedly connected, or integral；Can be mechanical connection, it can also
It is electrical connection；Can be directly connected, can also indirectly connected through an intermediary, can be connection inside two elements or
The interaction relationship of two elements.For the ordinary skill in the art, can understand as the case may be above-mentioned
The concrete meaning of term in the present invention.
Embodiment 1.
Referring to figure Fig. 2.
Embodiment 1 provides a kind of Cell proliferation device 1-6 for cell Proliferation system, as shown in Fig. 2, the cell Proliferation
Device 1-6 includes cell culture chamber 1-6-1, and microsphere supported 1-6-2 is placed in cell culture chamber 1-6-1, microsphere supported 1-6-2's
Ball particle size be 100 μm of -2mm, the microsphere supported material be selected from high molecular polymer, hydrogel, ceramics, metal, glass,
One or more in silica, certainly microsphere supported material can also use other high molecular polymers, on condition that the high score
The high stability of sub- polymer, seldom in cell culture fluid compound and intracellular biomass react, to
Ensure the stability of cell culture.
Can cell Proliferation be used for, microsphere supported by injection cell culture fluid in microsphere supported filled space
Top is provided with separation layer 1-6-3, culture space is isolated close on the position of the oral area of cell culture chamber 1-6-1.
Seal closure is set in the oral area of cell culture chamber 1-6-1, on the one hand reinforces seal, it can on another aspect seal closure
Connector is arranged, it is convenient for the access of catheter.
Embodiment 2.
With reference to attached drawing 1.
As shown in Figure 1, embodiment 2 provides a kind of automatic cytological proliferating system of individuation, including fixing module 1-1,
Cell Proliferation module 1-6, liquid storage module 1-2 and abandon liquid module 1-7.
The fixing module 1-1 includes that the first fixing presss from both sides 1-1-1, the second fixing folder 1-1-2 and fixing pedestal 1-1-3；Institute
It includes liquid storage cylinder 1-2-1 to state liquid storage module 1-2, and the exit of liquid storage cylinder 1-2-1 is equipped with breather valve 1-2-2, breather valve 1-2-2's
Effect is by the inside of liquid storage cylinder 1-2-1 and convenient in liquid storage cylinder 1-2-1；Cell Proliferation module includes one in the present embodiment
A Cell proliferation device 1-6 as described in Example 1, the liquid module 1-7 that abandons includes abandoning liquid reclaiming chamber 1-7-1.
In the present embodiment, it is connected to interface 1-3 by catheter 1-4 between the chamber of modules, 1-4 points of catheter
For for feed liquor the first catheter 1-4-1 and be used for out the second catheter 1-4-2 of liquid, liquid can will be abandoned by siphonage
It is sucked out.For example, the exit of liquid storage cylinder 1-2-1 passes through first interface 1-3-1 connection the first catheter 1-4-1, the first catheter
The other end of 1-4-1 is connected to cell culture chamber by second interface 1-3-2, extend into cell culture chamber 1-6-1, and pass through
Separation layer 1-6-3 is extend into the microsphere supported regions 1-6-2, and the oral area of Cell proliferation device 1-6 passes through third interface 1-
The other end of 3-3 the second catheters of connection 1-4-2, the second catheter 1-4-2 are connected to by the 4th interface 1-3-4 abandons liquid recycling
Chamber 1-7-1 forms the access of a cell culture fluid.
In order to preferably control cell cultivation process, flow regulator 1-5 control cell trainings can be set on catheter 1-4
The flow velocity of nutrient solution, specifically, flow regulator 1-5 include the first flow regulator being arranged on the first catheter 1-4-1
The 1-5-1 and second flow speed adjuster 1-5-2 being arranged on the second catheter 1-4-2, that is to say, that the liquid flow of injection process
Speed and the flow rate of liquid for abandoning liquid process can be separately adjustable.
In the present embodiment, the liquid storage module 1-2 and cell Proliferation module 1-6 and abandon liquid module 1-7 from top to bottom according to
Secondary connection, therefore, in the present embodiment, the cell culture fluid in liquid storage module 1-2 can automatically flow into carefully under the effect of gravity
Born of the same parents are proliferated in module, do not need external impetus equipment.Integrated support of the fixing module 1-1 as system, wherein liquid storage module 1-
Liquid storage cylinder 1-2-1 in 2 is pressed from both sides by the first fixing on the fixing holder that 1-1-1 is mounted in fixing module 1-1, cell Proliferation mould
Cell culture chamber 1-6-1 in block 1-6 presss from both sides 1-1-2 by the second fixing and is mounted on fixing holder, abandons abandoning in liquid module 1-7
Liquid reclaiming chamber 1-7-1 is placed on the fixing pedestal 1-1-3 of fixing module 1-1.
Above-mentioned, the whole height of the fixing module 1-1 is 20-50cm, width 12-30cm；The interface 1-3 bores are
1-5mm；The catheter 1-4 length range ranges 20-50cm.
Embodiment 3, with reference to attached drawing 3.
A kind of automatic cytological proliferating system of mass is present embodiments provided, as shown in figure 3, batch in the present embodiment
The cell Proliferation system of quantization abandons liquid module based on the cell Proliferation system of individuation, by a liquid storage module and one
The forms of the upper multiple Cell proliferation devices of parallel connection is realized, specifically, is responsible for the first catheter 1- of feed liquor in catheter 1-4
There are 4-1 multiple feed liquor branches, these feed liquor branches to be respectively protruding into cell Proliferation mould each cell culture chamber 1-6-1 in the block
Interior, also there are the second catheter 1-4-2 for being responsible for recycling in catheter 1-4 multiple Chu Ye branches, these Chu Ye branches to connect respectively
The sealing part of each cell culture chamber 1-6-1 is connect, the collection of waste cell culture solution is carried out.
The other embodiment of the present embodiment is same as Example 2.
Embodiment 4, with reference to attached drawing 3.
The present embodiment is also provided that a kind of automatic cytological proliferating system of mass, the present embodiment in embodiment 3
Mass system the difference is that, the present embodiment is in lateral arrangement on the whole, this is primarily to suitable for longitudinal high
The case where degree not enough is inconvenient to be vertically arranged, under this arrangement, liquid storage module 1-1 is arranged in side, cell Proliferation mould
Block 1-3 is arranged in centre, lower section or the other side that liquid module 1-7 is arranged in cell Proliferation module 1-3 is abandoned, due to liquid storage module 1-
Cell culture fluid in 1 can not flow directly into cell Proliferation module 1-1, it is therefore desirable to increase in liquid storage module 1-1 and cell
Grow addition power plant 1-8 between module 1-3.
The other embodiment of the present embodiment is same as Example 3.
Embodiment 5, the automation stem cells hyperplasia system having both the present embodiment provides a kind of mass and individuation are specific
Application process applies any one system in above-described embodiment 2-4 to carry out stem cell optimization proliferation.
Concrete operation step is as follows：
1, microsphere supported addition
1. in Biohazard Safety Equipment, the sterilized microspheres that size is 100-1000 μm are chosen
Carrier is positioned in cell Proliferation module.
2. being inoculated with a certain number of stem cells on demand on microsphere supported
2, it uses
1. prepared cell culture medium is positioned over liquid storage module.
2. opening flow regulator 1 and 2, culture solution is made to be full of entire cell Proliferation module.
Cell culture medium dynamic is allowed to replace such as 10-50 μ L/min to suitable range 3. adjusting flow velocity.
It is cultivated in carbonated (5%) incubator 4. moving to whole system.
5. according to being carried out needed for experiment, cell is single or batch collection.
It is above-mentioned, it is to be understood that the automation stem cells hyperplasia system that mass and individuation have both, it can be efficiently to dry
Cell sample is expanded, and has the characteristics that simple, cheap, sensitive, automation, possesses huge market-oriented foreground.
It is understood that the automation stem cells hyperplasia system structure that mass and individuation have both can hold 1
Also parallel multiple cell Proliferation modules can be held, you can set up multiple hepatocyte growth modules, and shared 1 by the way that catheter is parallel
It is a or multiple abandon liquid module mass proliferating stem cells
It is understood that the automation stem cells hyperplasia system that mass and individuation have both can equally be equipped with power
Support system (such as digitizes computer manipulation, microcontroller operation, peristaltic pump operating).In addition to different medical unit can be applied, item
In the case that part allows, it can equally be operated in specialized laboratory.
It is to be appreciated that this patent choose stem cell expanded, can under microsphere supported optimal conditions efficient amplification
Stem cell has energetically meaning to the treatment of clinical stem cell biological and cell transplantation.The foregoing is merely the utility model
Preferred embodiment is not intended to limit the utility model, and for those skilled in the art, the utility model can be with
There are various modifications and variations.Within the spirit and principle of the utility model, any modification, equivalent replacement, improvement
Deng should be included within the scope of protection of this utility model.
Claims (9)
1. the automation stem cells hyperplasia system that a kind of mass and individuation have both, it is characterised in that：Including fixing module, carefully
Born of the same parents are proliferated module, liquid storage module and abandon liquid module；Wherein cell Proliferation module includes at least one Cell proliferation device；It is described solid
Module is held for cell Proliferation module, liquid storage module to be installed or placed and abandons liquid module；The liquid storage module include liquid storage cylinder and
Breather valve；The cell Proliferation module includes cell culture chamber, and cell culture intracavitary fills microsphere supported, microsphere supported top cloth
Separation layer is set, the oral area of cell culture chamber is sealed with sealing cover；The liquid module of abandoning includes abandoning liquid reclaiming chamber；The liquid storage cylinder and
Between cell culture chamber, cell culture chamber and abandoning between liquid reclaiming chamber connected by catheter.
2. the automation stem cells hyperplasia system that a kind of mass as described in claim 1 and individuation have both, feature exist
In：The fixing module includes fixing pedestal, and fixing pedestal is equipped with fixing holder, and liquid storage cylinder passes through first clamping Dingan County of fixing
On fixing holder, cell culture chamber is fixedly mounted on by the second fixing folder on fixing holder, is abandoned liquid reclaiming chamber and is placed in admittedly
It holds on pedestal., the material of the fixing module is selected from metal, high molecular polymer.
3. the automation stem cells hyperplasia system that a kind of mass as described in claim 1 and individuation have both, feature exist
In：Cell culture fluid is housed, the amount of cell culture fluid is installed in addition with for 100-1000ml/ cells sets, the ventilation in the liquid storage cylinder
Valve ensures that cell culture fluid can smoothly flow out liquid storage cylinder, passes through first on liquid storage cylinder to connect ambient atmosphere and liquid storage cylinder
Interface connects the first catheter, and the other end of the first catheter connects cell proliferation chamber by second interface, on the first catheter
Equipped with the first flow regulator.
4. the automation stem cells hyperplasia system that a kind of mass as claimed in claim 3 and individuation have both, feature exist
In：The volume of Single cell culture chamber is 100ml；The oral area of the cell culture chamber connects the second drain by third interface
Pipe, the second catheter are stretched into across separation layer in microsphere supported, and the other end of the second catheter abandons liquid by the connection of the 4th interface
Culture chamber, the second catheter are equipped with second flow speed adjuster.
5. the automation stem cells hyperplasia system that a kind of mass as claimed in claim 4 and individuation have both, feature exist
In：The microsphere supported ball particle size is 100 μm of -2mm, and the microsphere supported material is selected from high molecular polymer, water
It is one or more in gel, ceramics, metal, glass, silica.
6. the automation stem cells hyperplasia system that a kind of mass as described in claim 1 and individuation have both, feature exist
In：It is described that abandon the liquid volume that liquid reclaiming chamber can accommodate be 100-1000ml/ Cell proliferation devices.
7. the automation stem cells hyperplasia system that a kind of mass as claimed in claim 4 and individuation have both, feature exist
In：The one kind or more of first catheter and the second catheter material in polyvinyl chloride, PE, PP plastics
Kind.
8. the automation stem cells hyperplasia system that a kind of mass as described in claim 1 and individuation have both, feature exist
In：The liquid storage cylinder abandons liquid reclaiming chamber and cell culture chamber material using sterile macromolecule polymer material, including poly- diformazan
Radical siloxane PDMS, acrylonitrile-butadiene-styrene copolymer, polycarbonate, polymethyl acrylate PMMA, polyethylene, gather
Propylene, polymethylpentene, polystyrene and nylon.
9. the automation stem cells hyperplasia system that a kind of mass as claimed in claim 4 and individuation have both, feature exist
In：The flow rate of liquid that first flow regulator and second flow speed adjuster are adjusted 1-3000 μ L ranging from per minute.
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Cited By (1)
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| CN108018208A (en) * | 2017-11-08 | 2018-05-11 | 浙江大学 | The automation stem cells hyperplasia system that a kind of mass and individuation have both |
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