CN210560380U - Vacuum concentration and purification treatment device - Google Patents
Vacuum concentration and purification treatment device Download PDFInfo
- Publication number
- CN210560380U CN210560380U CN201921190007.9U CN201921190007U CN210560380U CN 210560380 U CN210560380 U CN 210560380U CN 201921190007 U CN201921190007 U CN 201921190007U CN 210560380 U CN210560380 U CN 210560380U
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- luer
- universal
- plug
- vacuum
- luer interface
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- 238000000746 purification Methods 0.000 title claims abstract description 23
- 238000001179 sorption measurement Methods 0.000 claims abstract description 26
- 239000007788 liquid Substances 0.000 claims abstract description 18
- 238000009489 vacuum treatment Methods 0.000 claims abstract description 17
- 238000012545 processing Methods 0.000 claims description 16
- 208000001848 dysentery Diseases 0.000 claims description 10
- 238000003780 insertion Methods 0.000 claims description 9
- 230000037431 insertion Effects 0.000 claims description 9
- 239000011159 matrix material Substances 0.000 claims description 3
- 238000002474 experimental method Methods 0.000 abstract description 22
- 239000002699 waste material Substances 0.000 abstract description 9
- 230000000694 effects Effects 0.000 abstract description 4
- 238000005119 centrifugation Methods 0.000 abstract 1
- 238000011109 contamination Methods 0.000 abstract 1
- 150000007523 nucleic acids Chemical class 0.000 description 8
- 102000039446 nucleic acids Human genes 0.000 description 8
- 108020004707 nucleic acids Proteins 0.000 description 8
- 238000000034 method Methods 0.000 description 6
- 239000003153 chemical reaction reagent Substances 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 239000012488 sample solution Substances 0.000 description 4
- 238000000605 extraction Methods 0.000 description 3
- 239000013612 plasmid Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 238000001821 nucleic acid purification Methods 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 239000000523 sample Substances 0.000 description 2
- 239000002904 solvent Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 241000700605 Viruses Species 0.000 description 1
- 239000011543 agarose gel Substances 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000011027 product recovery Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 238000010977 unit operation Methods 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
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Abstract
本实用新型公开了一种真空浓缩纯化处理装置，包括：真空处理本体、鲁尔接口胶塞、鲁尔接口塞盖、通用型吸附柱和通用型注射器，真空处理本体表面设有多个鲁尔接口插槽；鲁尔接口胶塞可拆卸地设置于鲁尔接口插槽上；鲁尔接口塞盖与鲁尔接口胶塞相适配；通用型吸附柱用于插接鲁尔接口胶塞或将鲁尔接口胶塞设置在通用型吸附柱的上端；通用型注射器可拆卸连接在通用型吸附柱上端的鲁尔接口胶塞上。本实用新型根据具体实验选择鲁尔接口插槽的数目和位置，不用的鲁尔接口插槽用鲁尔接口胶塞和鲁尔接口塞盖塞上，保证实验气密性，满足多种实验需求；减少实验过程中废液倒弃的手动操作，降低污染概率；启动后瞬间完成和离心操作相同的效果，省时省力。
The utility model discloses a vacuum concentration and purification treatment device, comprising: a vacuum treatment body, a Luer interface rubber stopper, a Luer interface plug cover, a universal adsorption column and a universal syringe. The surface of the vacuum treatment body is provided with a plurality of Luer Interface slot; Luer interface rubber plug is detachably arranged on the Luer interface rubber plug; Luer interface plug cover is compatible with Luer interface rubber plug; Universal adsorption column is used for inserting Luer interface rubber plug or The Luer interface rubber stopper is arranged on the upper end of the universal adsorption column; the universal type syringe is detachably connected to the Luer interface rubber stopper at the upper end of the universal adsorption column. The utility model selects the number and position of the Luer interface slots according to specific experiments, and the unused Luer interface slots are plugged with Luer interface rubber plugs and Luer interface plug caps to ensure the air tightness of the experiment and meet various experimental requirements. ;Reduce the manual operation of discarding waste liquid during the experiment and reduce the probability of contamination; the same effect as the centrifugation operation is completed instantly after startup, saving time and effort.
Description
Technical Field
The utility model relates to a basic scientific research field, concretely relates to vacuum concentration purification processing apparatus.
Background
Purification, i.e., the process of converting an aggregate of multiple substances into one species or substance by physical, chemical, or biological means. Concentration is a unit operation that removes a portion of the solvent from the solution, and is a process in which the solute and solvent are partially separated.
In the experiment, because the content of impurities in the extracting solution is high and the concentration is low, the extracting solution is required to be refined, concentrated or purified. Such as nucleic acid purification kit, plasmid extraction kit, agarose gel or PCR product recovery kit, etc., are all designed by using the principle that an adsorption column adsorbs nucleic acid under the condition of high salt and elutes nucleic acid under the condition of low salt.
At present, the extraction and purification of DNA and RNA are mostly completed by a small-sized centrifuge through centrifugal operation, and the following problems exist:
(1) strict balancing and symmetrical placement are required during centrifugal operation, and the experimental steps are complicated;
(2) the waste liquid in the experimental process is manually poured to increase the pollution probability;
(3) the extraction and purification of nucleic acid of a large batch of samples are time-consuming and labor-consuming and are prone to errors;
(4) the experimental equipment has a complex structure, high experimental cost and time and labor waste.
In view of this, it is urgently needed to improve the existing concentration and purification treatment device, simplify the structure, reduce the cost and improve the experimental efficiency.
SUMMERY OF THE UTILITY MODEL
The utility model aims to solve the technical problem that current concentration and purification processing apparatus experimental procedure is loaded down with trivial details, and the manual increase pollution probability that falls of waste liquid in the experimentation, experimental facilities structure is complicated, and experiment is with high costs and the problem that wastes time and energy.
In order to solve the technical problem, the utility model provides a vacuum concentration and purification processing device, include:
the vacuum processing body is of a hollow structure, and the upper surface of the vacuum processing body is provided with a plurality of Ruhr interface slots;
the luer plug is detachably arranged on the luer slot;
the luer plug cover is matched with the luer rubber plug;
the universal adsorption column is used for plugging the luer rubber plug or arranging the luer rubber plug at the upper end of the universal adsorption column;
and the universal injector is detachably connected to the luer interface rubber plug at the upper end of the universal adsorption column.
In the above scheme, the luer slots are arranged in a matrix form and are arranged in 3 rows and 12 columns.
In the scheme, the left side of the vacuum treatment body is provided with a liquid outlet, the right side of the vacuum treatment body is provided with an adjustable gas port, and the bottom of the vacuum treatment body is provided with an anti-slip pad.
In the scheme, the number of the anti-slip pads is two, the anti-slip pads are arranged in a strip shape, and the length direction of the anti-slip pads is consistent with that of the vacuum treatment body.
In the above scheme, the liquid outlet can be dismantled and be connected the pipette, the other end of pipette is connected vacuum force pump.
In the scheme, the diameter of the luer insertion slot is 7mm, the inner caliber of the luer rubber plug is 4mm, the outer caliber of the luer rubber plug is 7mm, the diameter of the luer plug cover is 4.3mm, the diameter of the bottom insertion end of the universal adsorption column is 4mm, and the connecting caliber of the universal injector is 4 mm.
In the above scheme, the vacuum processing body is made of PP material.
Compared with the prior art, the utility model has the advantages of it is following:
1. the universal type adsorption column splicing mode is adopted, the operation is simple, and the experiment efficiency is effectively improved;
2. the number and the positions of the Ruhr interface slots can be selected according to specific experimental requirements, and the unused Ruhr interface slots are plugged by a Ruhr interface rubber plug and a Ruhr interface plug, so that the air tightness of the experiment is ensured, and various experimental requirements are met;
3. manual operation of waste liquid dumping in the experimental process is reduced, and the pollution probability is reduced;
4. the vacuum treatment body can be used for multiple times, so that the cost is reduced, the effect same as that of centrifugal operation can be instantly finished after the vacuum treatment body is started, and time and labor are saved;
5. the whole device has few accessories, simple structure and low cost.
Drawings
FIG. 1 is a schematic view of a vacuum concentration and purification treatment apparatus of the present invention;
figure 2 is the front view of the luer plug of the present invention inserted into a luer plug.
Detailed Description
The utility model provides a vacuum concentration and purification treatment device, simple structure, the experiment is with low costs. The invention is described in detail below with reference to the drawings and the detailed description.
As shown in fig. 1, the vacuum concentration and purification apparatus provided by the present invention comprises a vacuum processing body 10, a luer plug 20, a luer plug cover 30, a universal adsorption column 40 and a universal injector 50.
The vacuum processing body 10 is a hollow structure, and the upper surface of the vacuum processing body is provided with a luer insertion slot 11. During the experiment, the waste liquid flows into the vacuum treatment body 10 through the luer insertion slot 11.
The luer plug 20 is detachably arranged on the luer slot 11, and is used during experiments to ensure that the periphery of the luer slot 11 is sealed.
The luer plug 30 is removably disposed on the luer plug 20. The air-tightness of the experiment is ensured by plugging the air-tightness test plug on a luer slot 11 which is not used in the experiment.
The universal adsorption column 40 is a consumable in a nucleic acid purification reagent kit in a laboratory, and is inserted into the luer rubber plug 20 for use during an experiment. The upper end is adapted to the luer plug 20 for concentration.
The universal syringe 50 is detachably connected to the luer plug 20 at the upper end of the universal adsorption column 40. The general-purpose syringe 50 is used to place a large volume of sample solution or reagent for concentration processing.
Preferably, the luer slots 11 are arranged in a matrix form and are arranged in 3 rows and 12 columns, and as the experiment among the luer slots 11 has no influence, 36 samples can be tested at most simultaneously, and the experiment efficiency is high.
The left side of vacuum treatment body 10 is equipped with liquid outlet 13, and the right side is equipped with adjustable gas port 14, and the bottom is equipped with slipmat 12, and slipmat 12 is two bar slipmats, and length direction is unanimous with the length direction of vacuum treatment body 10, and the position is just to the bottom in the horizontal space of luer interface slot 11, because the slip of device influences the experimental result during the avoidance experiment.
The liquid outlet 13 of the vacuum processing body 10 is detachably connected with a pipette 61 of the vacuum pressure pump 60. The other end of the pipette 61 is connected to a vacuum pressure pump 60. The vacuum pressure pump 60 is used to pump out the air inside the vacuum processing body 10, so that the sample solution or the test reagent in the universal adsorption column 40 flows out from the bottom end thereof.
The diameter of the luer insertion slot 11 is 7mm, the inner caliber of the luer plug 20 is 4mm, the outer caliber of the luer plug is 7mm, the diameter of the luer plug cover 30 is 4.3mm, the diameter of the bottom insertion end of the universal adsorption column 40 is 4mm, and the connecting caliber of the universal injector 50 is 4 mm. The size is set accurately, and the effect of each part in the experiment is guaranteed.
Wherein, preferably, the vacuum treatment body is made of PP material, has low manufacturing cost and can be repeatedly used after high-temperature sterilization.
The utility model discloses a use method as follows:
(1) purification of nucleic acid material
During the experiment, the device is placed on a flat experiment table, and the liquid outlet 13 of the vacuum treatment body 10 is arranged on the left side; the luer plug 20 is arranged in the luer slot 11, the lower end of the universal adsorption column 40 is inserted into the luer plug 20, and the unused luer plug 20 is plugged into the luer plug cover 30; transferring the sample solution or the experimental reagent into the universal adsorption column 40; then, a pipette 61 of the vacuum pressure pump 60 is connected with the liquid outlet 13, the air port 14 can be adjusted to be appropriately screwed, the vacuum pressure pump 60 is started to suck out the waste liquid, and the flow rate can be controlled by adjusting the vacuum pressure pump 60; purified nucleic acid material was obtained according to the relevant kit instructions.
(2) Concentration and purification of large-volume low-copy-number plasmid or large-volume low-load viral nucleic acid
The device is placed in the same way as (1), the interface of the universal injector 50 is connected with the luer rubber plug 20 and is placed on the universal adsorption column 40 together, the sample solution to be treated is moved into the universal injector 50, the vacuum pressure pump is started to suck out the waste liquid, the universal adsorption column 40 is washed according to the instruction of the relevant kit, and finally, a proper amount of TE liquid is added into the universal adsorption column 40 to elute the sample nucleic acid on the adsorption column 40, so that the concentration and purification treatment of the sample are completed.
The utility model has the advantages of it is following:
1. the universal type adsorption column splicing mode is adopted, the operation is simple, and the experiment efficiency is effectively improved;
2. the number and the positions of the Ruhr interface slots can be selected according to specific experimental requirements, and the unused Ruhr interface slots are plugged by a Ruhr interface rubber plug and a Ruhr interface plug, so that the air tightness of the experiment is ensured, and various experimental requirements are met;
3. manual operation in the experimental process is reduced, and the pollution probability is reduced;
4. the vacuum treatment body can be used for multiple times, so that the cost is reduced, the effect same as that of centrifugal operation can be instantly finished after the vacuum treatment body is started, and time and labor are saved;
5. the whole device has few accessories, simple structure and low cost;
6. the universal centrifugal column and the injector are connected through the luer rubber plug, so that the concentration of large-volume low-copy-number plasmid or low-load virus nucleic acid is realized.
The present invention is not limited to the above-mentioned best mode, and any person should learn the structural change made under the teaching of the present invention, all with the present invention has the same or similar technical solution, all fall into the protection scope of the present invention.
Claims (7)
1. A vacuum concentration and purification treatment device is characterized by comprising:
the vacuum processing body is of a hollow structure, and the upper surface of the vacuum processing body is provided with a plurality of Ruhr interface slots;
the luer plug is detachably arranged on the luer slot;
the luer plug cover is matched with the luer rubber plug;
the universal adsorption column is used for plugging the luer rubber plug or arranging the luer rubber plug at the upper end of the universal adsorption column;
and the universal injector is detachably connected to the luer interface rubber plug at the upper end of the universal adsorption column.
2. The vacuum concentration and purification processing device as claimed in claim 1, wherein the luer insertion slots are arranged in a matrix arrangement of 3 rows and 12 columns.
3. The vacuum concentration and purification treatment device according to claim 1, wherein the vacuum treatment body is provided with a liquid outlet at the left side, an adjustable air port at the right side and a non-slip mat at the bottom.
4. The vacuum concentration and purification treatment device according to claim 3, wherein the number of the anti-slip pads is two, the anti-slip pads are arranged in a strip shape, and the length direction of the anti-slip pads is consistent with the length direction of the vacuum treatment body.
5. The vacuum concentration and purification treatment device as claimed in claim 3, wherein the liquid outlet is detachably connected with a liquid suction pipe, and the other end of the liquid suction pipe is connected with a vacuum pressure pump.
6. The vacuum concentration and purification treatment device according to claim 1, wherein the luer insertion groove has a diameter of 7mm, the luer plug has an inner diameter of 4mm and an outer diameter of 7mm, the luer plug cover has a diameter of 4.3mm, the bottom insertion end of the universal adsorption column has a diameter of 4mm, and the universal syringe has a connection diameter of 4 mm.
7. The vacuum concentration and purification apparatus as claimed in claim 1, wherein the vacuum processing body is made of PP.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921190007.9U CN210560380U (en) | 2019-07-26 | 2019-07-26 | Vacuum concentration and purification treatment device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921190007.9U CN210560380U (en) | 2019-07-26 | 2019-07-26 | Vacuum concentration and purification treatment device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN210560380U true CN210560380U (en) | 2020-05-19 |
Family
ID=70676419
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201921190007.9U Expired - Fee Related CN210560380U (en) | 2019-07-26 | 2019-07-26 | Vacuum concentration and purification treatment device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN210560380U (en) |
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2019
- 2019-07-26 CN CN201921190007.9U patent/CN210560380U/en not_active Expired - Fee Related
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20200519 Termination date: 20210726 |
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| CF01 | Termination of patent right due to non-payment of annual fee |