KR102258768B1 - Apparatus and method for nucleic acid extraction and amplification - Google Patents

Abstract

The nucleic acid extraction and gene amplification apparatus includes a body part, a seating part arranged in a circular shape on the body part, a driving part that is raised and lowered from an upper part of the body part, and a rotating part connected to the driving part and rotating the driving part in one direction, and the driving part contains a solvent. A solvent container that accommodates and supplies a solvent to the seating part, an electromagnet bar that intersects with the solvent container and moves a sample containing nucleic acids, and is located opposite to the solvent container, and is located opposite to the pressure bar and the electromagnet bar that apply pressure to the seating part. It may include a lead bar.

Description

Nucleic acid extraction and gene amplification apparatus and method {APPARATUS AND METHOD FOR NUCLEIC ACID EXTRACTION AND AMPLIFICATION}

A nucleic acid extraction and gene amplification apparatus and method are provided.

In general, it is necessary to extract and analyze nucleic acids from samples containing cells in various fields such as analysis of the cause of diseases through the results of human genome research and preliminary testing for virus or bacterial infection.

Conventional devices for analyzing nucleic acids require the operator to transfer the extracted nucleic acids in a separate tube and transfer them directly to a polymerase chain reaction (PCR) device to sequentially conduct and analyze experiments in different reactors. Is reduced, and user discomfort may occur. In addition, analysis equipment such as PCR equipment and electrophoresis are required separately, and since reagents are used in stages, high cost may occur. In addition, in the process of processing a large number of samples, contamination due to each sample may occur, and thus, treatment efficiency may be lowered.

An embodiment of the present invention is to provide a nucleic acid extraction and gene amplification apparatus and method capable of separating and amplifying a nucleic acid from a sample using a driving unit and a rotating unit, so that from extraction to amplification of the nucleic acid can be performed at once.

In addition to the above problems, embodiments according to the present invention may be used to achieve other tasks not specifically mentioned.

The nucleic acid extraction and gene amplification apparatus according to an embodiment of the present invention includes a body part, a seating part arranged in a circular shape on the body part, a driving part that is raised and lowered from the upper part of the body part, and a rotating part connected to the driving part and rotating the driving part in one direction. Including, the driving unit is a solvent container for receiving the solvent and supplying the solvent to the seating unit, an electromagnet bar positioned to cross the solvent container and moving the sample containing nucleic acid, and positioned opposite to the solvent container and applying pressure to the seating unit. It may include a pressure bar and a lead bar positioned to face the electromagnet bar.

The seating portion may include a nucleic acid tube inserted into the seating portion and supplied with a sample containing a nucleic acid and containing a binding buffer. The seating unit may include an electrode positioned under the nucleic acid tube and applying power to the sample to extract nucleic acid from the sample. The seating portion may include a PCR tube inserted into the seating portion, arranged in a circular shape in the seating portion, supplied with a nucleic acid separated from the sample, and containing an elution buffer.

The PCR tube may include an extraction tube including a filter therein and an amplification tube coupled to a lower portion of the extraction tube. The seating part may include a heater located under the PCR tube and controlling the temperature of the PCR tube and amplifying the nucleic acid separated from the sample. The seating portion may include a washing tube inserted into the seating portion and receiving a washing liquid to wash the sample. The seating portion may include a heating tube inserted into the seating portion and evaporating the washing liquid.

The nucleic acid extraction and gene amplification method according to an embodiment of the present invention includes a step of supplying a sample containing a nucleic acid to a nucleic acid tube containing a binding buffer, applying an electrical stimulation to the sample by an electrode located under the nucleic acid tube, The step of separating the nucleic acid from the sample, the step of lowering the solvent container toward the nucleic acid tube, the step of supplying the solvent to the nucleic acid tube by the solvent container, the step of binding the nucleic acid and the magnetic beads by the binding buffer, the step of the solvent container rising and the electromagnet The step of lowering the bar toward the nucleic acid tube, the step of holding the magnetic bead and the nucleic acid to which the electromagnet bar is bound and moving it to the washing tube containing the washing solution, the step of washing the bound nucleic acid and magnetic bead with the washing solution, and the electromagnet bar lowering toward the washing tube And moving the combined nucleic acid and magnetic beads to a heating tube, evaporating the washing solution containing the combined nucleic acid and magnetic beads, the electromagnet bar descending toward the heating tube, and the combined nucleic acid and magnetic beads are subjected to PCR containing the elution buffer. It may include moving to a tube, separating the bound nucleic acid from the magnetic beads, and moving the electromagnet bar downwards toward the PCR tube and moving the magnetic beads to the storage tube, and amplifying the nucleic acid contained in the PCR tube. .

The steps in which the nucleic acid contained in the PCR tube is amplified include: the pressure bar descends toward the PCR tube, the nucleic acid passes through the filter and moves to the amplification tube, the heater controls the temperature of the amplification tube, and the nucleic acid controls the temperature. It may include a step of being amplified by.

In one embodiment of the present invention, since the nucleic acid extraction to amplification can be performed at once, sample processing efficiency can be improved.

According to an embodiment of the present invention, since the process of performing experiments in different reactors directly transferred by an operator can be omitted, contamination or interference between tubes can be prevented in advance.

1 is a perspective view showing a nucleic acid extraction and gene amplification apparatus according to an embodiment of the present invention.
2 is a perspective view showing a body portion and a seating portion of a nucleic acid extraction and gene amplification apparatus according to an embodiment of the present invention.
3 is a perspective view showing a driving unit and a rotating unit of a nucleic acid extraction and gene amplification apparatus according to an embodiment of the present invention.
4 to 10 are cross-sectional views illustrating an apparatus for extracting a nucleic acid and amplifying a gene according to it.

The terminology used herein is for reference only to specific embodiments and is not intended to limit the present invention. Singular forms as used herein also include plural forms unless the phrases clearly indicate the opposite. The meaning of "comprising" as used in the specification specifies a specific characteristic, region, integer, step, action, element and/or component, and other specific characteristic, region, integer, step, action, element, component and/or group It does not exclude the existence or addition of

Although not defined differently, all terms including technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which the present invention belongs. Terms defined in a commonly used dictionary are additionally interpreted as having a meaning consistent with the related technical literature and the presently disclosed content, and are not interpreted in an ideal or very formal meaning unless defined.

Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings so that those of ordinary skill in the art may easily implement the present invention. However, the present invention may be implemented in various different forms and is not limited to the embodiments described herein.

1 shows a perspective view of a nucleic acid extraction and gene amplification apparatus 1 according to an embodiment of the present invention. The structure of the nucleic acid extraction and gene amplification apparatus 1 of FIG. 1 is for illustrative purposes only, and the present invention is not limited thereto. Accordingly, the structure of the nucleic acid extraction and gene amplification device 1 can be modified in other forms.

Referring to FIG. 1, the nucleic acid extraction and gene amplification apparatus 1 may include a body part 10, a seating part 20, a driving part 30, and a rotating part 40. The main body 10 may have a plate shape. The body portion 10 may include a through hole 11, a column portion 12, and a connection portion 13.

The through hole 11 may be located in the center of the main body 10. The through hole 11 may have a shape of a hole penetrating the body portion 10 in the z-axis direction. The drive shaft 38 may be inserted into the through hole 11.

The pillar portion 12 may be connected to the body portion 10. The pillar part 12 may extend in the z-axis direction. The pillar portions 12 may be connected to one side and the other side of the body portion 10 in a pair, respectively. The pillar part 12 may be located outside the radius of rotation of the driving part 30. When the driving part 30 rotates in one direction along the direction of the arrow, the column part 12 may protect the driving part 30.

The connection portion 13 may be positioned between the pair of pillar portions 12 and connect the pillar portions 12 to each other. The connection part 13 may include a connection hole 14. The connection hole 14 may have a hole shape penetrating the central portion of the connection part 13 in the z-axis direction. The upper end of the drive shaft 38 may be inserted into the connection hole 14.

1 and 2, the seating portion 20 may be located on the body portion 10. The seating portion 20 may have a groove shape concave in the inner direction of the body portion 10. The seating portion 20 may be positioned to be spaced apart from each other in a plurality. The seating portion 20 may be arranged in a circular shape around the through hole 11. The seating portion 20 may include a nucleic acid tube 21, a washing tube 22a, 22b, and 22c, a heating tube 23, a PCR tube 24, and a storage tube 27.

The nucleic acid tube 21 may be inserted into the seating portion 20. The nucleic acid tube 21 may contain a sample. The sample may be a genetic material composed of polynucleotides. The nucleic acid tube 21 may contain a sample including nucleic acid or the like. The sample may contain deoxyribonucleic acid (DNA) or ribonucleic acid (RNA).

The nucleic acid tube 21 may contain a binding buffer. The sample accommodated in the nucleic acid tube 21 may be separated into nucleic acids or proteins contained in the sample through electrical stimulation. Nucleic acid extracted from the sample can be bound to magnetic beads by binding buffer.

The cleaning tubes 22a. 22b, 22c may be inserted into the seating portion 20. The washing tubes 22a, 22b, 22c may contain a washing liquid. The washing liquid may be ethanol. The cleaning tube 22a may be located on the counterclockwise side of the nucleic acid tube 21 with the through hole 11 as the center.

The cleaning tube 22b may be located on the counterclockwise side of the cleaning tube 22a with the through hole 11 as the center. The washing tube 22b may contain a washing liquid. The cleaning tube 22c may be located on the counterclockwise side of the cleaning tube 22b with the through hole 11 as the center. The combined nucleic acid and magnetic beads may be washed with each of the washing tubes 22a. 22b and 22c while passing through the respective washing tubes 22a. 22b and 22c to increase purity.

The heating tube 23 may be inserted into the seating portion 20. The heating tube 23 may be located on the counterclockwise side of the cleaning tube 22c with the through hole 11 as the center. The heating tube 23 may evaporate the combined nucleic acid and the washing liquid remaining on the surface of the magnetic bead.

The PCR tube 24 may be inserted into the seating part 20. The PCR tube 24 may be located on the counterclockwise side of the heating tube 23 with the through hole 11 as the center. The PCR tube 24 may contain an elution buffer. The bound nucleic acid and magnetic beads may be separated from each other by an elution buffer.

The PCR tube 24 may amplify magnetic beads and separated nucleic acids. The PCR tubes 24 are spaced apart from each other in a plurality and may be arranged in a circle along the circumference of the PCR tube 24. Each of the PCR tubes 24 may accommodate different samples.

The storage tube 27 may be inserted into the seating portion 20. The storage tube 27 may be located on the counterclockwise side of the PCR tube 24 with the through hole 11 as the center. The storage tube 27 may accommodate a magnetic bead separated from the nucleic acid.

Referring to FIGS. 1 and 3, the driving unit 30 may be raised and lowered from the top of the main body 10. The driving unit 30 may include a solvent container 31, an electromagnet bar 32, a pressure bar 33, a lead bar 34, a motor 35, a guide rail 36, and a drive shaft 38. Since the solvent container 31, the electromagnet bar 32, the pressure bar 33, and the lead bar 34 can be connected to the motor 35, respectively, they can be independently raised and lowered along the z-axis direction.

The solvent container 31, the electromagnet bar 32, the pressure bar 33, and the lead bar 34 can be stably raised and lowered along the guide rail 36 positioned in the z-axis direction. The solvent container 31, the electromagnet bar 32, the pressure bar 33, and the lead bar 34 may rotate in one direction around the drive shaft 38.

The solvent container 31 may accommodate a solvent therein and supply the solvent to the seating portion 20. The solvent may be ethanol. The solvent container 31 descends toward the nucleic acid tube 21 and may supply ethanol into the nucleic acid tube 21.

The electromagnet bar 32 may be positioned intersecting with the solvent container 31. The electromagnet bar 32 lifts and descends on the nucleic acid tube 21, the washing tube 22, the heating tube 23, the PCR tube 24, or the storage tube 27, It is possible to hold and move the combination of beads.

The pressure bar 33 may be positioned to face the solvent container 31. The pressure bar 33 may be positioned intersecting with the electromagnet bar 32. The pressure bar 33 may apply pressure to the seating portion 20. The pressure bar 33 may descend toward the PCR tube 24 and apply pressure to the nucleic acid contained in the PCR tube 24.

The lead bar 34 may be positioned to face the electromagnet bar 32. The lead bar 34 may be positioned intersecting with the pressure bar 33. When the nucleic acid contained in the PCR tube 24 is amplified, the lead bar 34 may descend toward the PCR tube 24 and may be inserted into the PCR tube 24. The lead bar 34 may serve as a lead of the PCR tube 24.

The rotating part 40 may be connected to the driving part 30. The rotation unit 40 may rotate the driving unit 30 in one direction. The rotating part 40 may include a first rotating body 41, a second rotating body 42, a chain 43 and a motor 44.

The first rotating body 41 may be connected to the lower portion of the drive shaft 38. The second rotating body 42 may be connected to the rotating shaft of the motor 44. The first rotating body 41 and the second rotating body 42 may be interconnected through a chain 43. The first rotating body 41 and the second rotating body 42 may have outer surfaces formed in a serrated shape or the like. The chain 43 may be a timing chain. The tooth shape of the first and second rotors 41 and 42 may rotate in forward and reverse directions according to the rotation of the motor 44 while being engaged with the timing chain. In this case, the driving unit 30 may rotate while the driving shaft 38 connected to the first rotating body 41 rotates.

The nucleic acid extraction and gene amplification device 1 includes a nucleic acid tube 21, a washing tube 22, a heating tube 23, a PCR tube 24 arranged in a circular shape while the driving unit 30 is raised and lowered and the rotating unit 40 rotates ) And it is possible to move the nucleic acid, magnetic beads, or a combination of nucleic acids and magnetic beads, respectively accommodated in the storage tube (27). Accordingly, since the nucleic acid extraction and gene amplification apparatus 1 can automatically perform a process of extracting, washing, and amplifying a nucleic acid from a sample according to steps, additional equipment can be omitted and processing efficiency can be improved.

Conventionally, a process of transferring nucleic acids into separate tubes was carried out by the operator, and analysis equipment such as PCR equipment and electrophoresis may cause contamination due to each sample separately, and treatment efficiency was low.

Then, a nucleic acid extraction and gene amplification method according to an embodiment of the present invention will be described.

Referring to FIG. 4, the nucleic acid tube 21 may contain a binding buffer (B1). A sample may be supplied to the nucleic acid tube 21. The electrode 28 may be located under the nucleic acid tube 21. The electrode 28 may be a needle-shaped electrode. In this case, the electrode 28 may be inserted into the lower end of the nucleic acid tube 21. The electrode 28 can directly apply an electric stimulation to the sample accommodated in the nucleic acid tube 21, so that the transferability of the electric stimulation can be improved.

As shown in the enlarged circle of FIG. 4, when power is applied to the electrode 28, electrical stimulation is generated on the sample accommodated in the nucleic acid tube 21, so that the nuclear film N of the sample may be killed or destroyed. Electrical stimulation of the electrode 28 destroys the nuclear membrane N in a short time, thereby reducing the extraction time of the nucleic acid D. Accordingly, the nucleic acid extraction and gene amplification device 1 can quickly diagnose a highly infectious disease.

In addition, the solvent container 31 may descend toward the nucleic acid tube 21 by driving the motor 35. The solvent container 31 may supply the solvent E contained therein to the nucleic acid tube 21. Nucleic acid (D) moved to the outside of the nuclear membrane (N) can be bound to the magnetic beads (M) by the binding buffer (B1). The solvent container 31 may rise in the z-axis direction by driving the motor 35 after supplying the solvent E to the nucleic acid tube 21.

Referring to FIG. 5, the electromagnet bar 32 may descend toward the nucleic acid tube 21 by driving the motor 35. The electromagnet bar 32 may rise in the z-axis direction while holding the combination of the nucleic acid (D) and the magnetic bead (M) accommodated in the nucleic acid tube 21 through an electromagnetic force. The electromagnet bar 32 may be rotated in one direction by the driving of the rotating part 40 to be positioned above the cleaning tube 22.

Referring to FIG. 6, the electromagnet bar 32 may descend toward the washing tube 22 while holding the combination of the nucleic acid (D) and the magnetic bead (M). The electromagnet bar 32 releases the electromagnetic force, and the combination of the nucleic acid (D) and the magnetic bead (M) may be accommodated in the washing tube 22. The washing tube 22 may contain the washing liquid (E). The conjugate of nucleic acid (D) and magnetic beads (M) can be washed with the washing liquid (E) of the washing tube 22 to increase purity. If necessary, the conjugate of the washed nucleic acid (D) and the magnetic bead (M) is sequentially moved to the washing tubes 22b and 22c to perform additional washing.

In this case, the electromagnet bar 32 holds the combination of the nucleic acid (D) and the magnetic beads (M), which was firstly washed in the cleaning tube 22, through electromagnetic force, and rotates by driving the rotation unit 40 to rotate the cleaning tube ( It can be located on top of 22b). The electromagnet bar 32 descends toward the cleaning tube 22b and releases the electromagnetic force, so that the combination of the nucleic acid (D) and the magnetic bead (M) can be washed secondarily.

In addition, the electromagnet bar 32 can be positioned at the top of the cleaning tube 22c by holding the combination of the secondary washed nucleic acid (D) and the magnetic bead (M) through electromagnetic force and rotating by the drive of the rotating unit 40. have. The electromagnet bar 32 descends toward the washing tube 22c and releases the electromagnetic force, so that the combination of the nucleic acid (D) and the magnetic bead (M) can be washed thirdly. The electromagnet bar 32 may rise in the z-axis direction while holding the combination of the washed nucleic acid (D) and the magnetic bead (M).

Referring to FIG. 7, the electromagnet bar 32 may be rotated in one direction by driving of the rotating part 40 to be positioned above the heating tube 23 and descend toward the heating tube 23. The electromagnet bar 32 releases an electromagnetic force, and a combination of the nucleic acid (D) and the magnetic bead (M) may be accommodated in the heating tube 23.

A heater part 29 may be included in the lower portion of the heating tube 23. The heater unit 29 may be positioned to surround the lower portion of the heating tube 23. The heater unit 29 may include an aluminum cover or a heating element. In this case, the heat generated from the heater unit 29 may be transferred to the heating tube 23 without loss by the aluminum cover or the heating element. The heater unit 29 may apply heat to a combination of the nucleic acid (D) and the magnetic beads (M) accommodated in the heating tube (23).

As shown in the enlarged circle of FIG. 7, the cleaning liquid E around or around the surface of the combination of the nucleic acid D and the magnetic beads M may be evaporated by the heat of the heater unit 29. In this case, since the washing liquid may be completely evaporated from the surface of the nucleic acid (D), the amplification efficiency of the nucleic acid (D) can be increased. The electromagnet bar 32 may rise in the z-axis direction while holding the combination of the nucleic acid (D) from which the washing liquid (E) has been removed and the magnetic bead (M).

Referring to FIG. 8, the electromagnet bar 32 rotates in one direction by the driving of the rotation unit 40 and is positioned above the PCR tube 24 and may descend toward the PCR tube 24. The electromagnet bar 32 may release the electromagnetic force to place the combination of nucleic acid (D) and magnetic bead (M) in the PCR tube 24 and then rise in the z-axis direction. The PCR tube 24 may contain an elution buffer (B2).

As shown in the enlarged circle on the right of FIG. 8, the conjugate of the nucleic acid (D) and the magnetic bead (M) may be separated from each other by the elution buffer (B2). As shown in the enlarged circle on the left of FIG. 8, the electromagnet bar 32 descends toward the PCR tube 24 to hold the separated magnetic bead M through an electromagnetic force and may rise again.

The electromagnet bar 32 is raised in the z-axis direction while holding only the separated magnetic bead (M), and is rotated in one direction by the drive of the rotating part 40 to be located above the storage tube 27 and the storage tube 27 You can descend towards. The magnetic bead (M) may be accommodated in the storage tube (27).

Referring to FIG. 9, since the magnetic beads M can be removed from the PCR tube 24 by the electromagnet bar 32, only the nucleic acid D can be accommodated in the PCR tube 24. The pressure bar 33 may be rotated in one direction by the drive of the rotating part 40 to be positioned above the PCR tube 24 and descend toward the PCR tube 24.

The pressure bar 33 may include a packing 37. The packing 37 may be made of rubber or the like. When the pressure bar 33 descends into the inside of the extraction tube 25, the packing 37 may seal the extraction tube 25.

Referring to FIG. 10, the PCR tube 24 may include an extraction tube 25 and an amplification tube 26. The lower portion of the extraction tube 25 may be coupled to the amplification tube 26. The lower portion of the extraction tube 25 may be located inside the amplification tube 26. The extraction tube 25 may include a filter (F). The nucleic acid (D) is adsorbed on the filter member and can move to the amplification tube 26. The filter F may include a syringe filter.

When the pressure bar 33 is inserted into the extraction tube 25, the air inside the extraction tube 25 can be compressed, so that the nucleic acid (D) located between the filter (F) and the pressure bar (33) is pressurized. can do. The lower end of the extraction tube 25 may be inclined in a direction toward the inside of the extraction tube 25. The nucleic acid (D) passing through the filter (F) can move along the slope to the amplification tube 26.

A heater unit 29 may be positioned under the amplification tube 26. The heater unit 29 may control the temperature of the amplifying tube 26. The heater unit 29 may perform an amplification process by repeating heating and cooling to a temperature suitable for amplification of the nucleic acid D accommodated in the amplification tube 26. The heater unit 29 may be a Peltier element. In this case, the heater unit 29 may be electrically connected to the external power source P. The heater unit 29 may have different temperatures on the upper surface and the lower surface. For example, when the upper surface of the heater unit 29 generates heat, the lower surface may be cooled, and when the upper surface of the heater unit 29 is cooled, the lower surface may generate heat. Accordingly, the heater unit 29 can easily control temperature, such as lowering the temperature during heat generation or increasing the temperature during cooling.

When the amplification process of the nucleic acid (D) is performed in the PCR tube 24, the pressure bar 33 may rise in the z-axis direction while holding the extraction tube 25. In addition, the lead bar 34 may be rotated in one direction by the driving of the rotating part 40 to be positioned above the amplifying tube 26. The lead bar 34 may descend toward the amplifying tube 26. The lead bar 34 may be inserted into the amplifying tube 26 to serve as a lid of the amplifying tube 26. The lead bar 34 may block water vapor that may be generated inside the amplifying tube 26 from being discharged to the outside of the amplifying tube 26.

The nucleic acid extraction and gene amplification device 1 is a nucleic acid (D), magnetic bead (M) or nucleic acid (D) in the process of nucleic acid extraction, washing, amplification, etc. by vertical and rotational driving of the electromagnet bar 32 in one device. ) And magnetic beads (M) can be moved automatically. In addition, since the solvent container 31, the pressure bar 33, and the lead bar 34 can be provided in each tube according to the process of nucleic acid extraction, washing, amplification, etc., additional equipment can be omitted, thereby reducing the cost of equipment. Saving and increasing sample processing efficiency. In addition, since the process of conducting experiments in different reactors by direct transfer by the operator can be omitted, contamination or interference between tubes or solutions can be prevented in advance.

Although the preferred embodiments of the present invention have been described in detail above, the scope of the present invention is not limited thereto, and various modifications and improvements by those skilled in the art using the basic concept of the present invention defined in the following claims are also present. It belongs to the scope of rights of

10: main body 11. through hole
12. Column 13. Connection
14. Connection hole 20. Seating part
21. Nucleic acid tube 22. Washing tube
23. Heating tube 24. PCR tube
25. Extraction tube 26. Amplification tube
27. Storage tube 28. Electrode
29. Heater part 30. Drive part
31. Solvent container 32. Electromagnet bar
33. Pressure bar 34. Lead bar
35. Motor 36. Guide rail
37. Packing 38. Drive shaft
40. Rotating part 41. First rotating body
42. Second rotation body 43. Chain
44. Motor B1. Binding buffer
B2. Elution buffer M. magnetic beads
N. Nuclear membrane F. Filter

Claims (10)

Body part,
A seating portion arranged in a circular shape on the body portion,
A driving unit that is elevating and descending from the upper portion of the main body, and
A rotating part connected to the driving part and rotating the driving part in one direction,
Including,
The driving unit,
A solvent container for receiving a solvent and supplying the solvent to the seating portion,
An electromagnet bar that intersects with the solvent container and moves a sample containing a nucleic acid,
A pressure bar facing the solvent container and applying pressure to the seating portion,
Lead bar located opposite to the electromagnet bar
Including,
The seating unit includes a nucleic acid tube to which a sample containing the nucleic acid is supplied, a washing tube positioned at a counterclockwise side of the nucleic acid tube and washing the sample, a PCR positioned at a counterclockwise side of the washing tube and accommodating an elution buffer A tube, and a storage tube located on the counterclockwise side of the PCR tube and accommodating a magnetic bead,
The solvent container, the pressure bar, and the electromagnet bar are sequentially positioned in a counterclockwise direction, rotated in the one direction around a drive shaft, each independently elevating and descending,
The electromagnet bar is sequentially raised and lowered in the nucleic acid tube, the washing tube, the PCR tube, and the storage tube,
The electromagnet bar rotates in the one direction and is located above the PCR tube and descends into the PCR tube, and the nucleic acid and the magnetic bead are separated by the elution buffer, and
The pressure bar rotates in the one direction and is located on the upper part of the PCR tube and descends into the PCR tube, and the separated nucleic acid is amplified,
Nucleic acid extraction and gene amplification device consisting of one device. In claim 1,
A nucleic acid extraction and gene amplification apparatus including the nucleic acid tube inserted into the seating portion and accommodating a binding buffer. In paragraph 2,
The mounting portion, a nucleic acid extraction and gene amplification apparatus including an electrode located under the nucleic acid tube and applying power to the sample to extract the nucleic acid of the sample. In paragraph 2,
The mounting portion, a nucleic acid extraction and gene amplification apparatus including the PCR tube inserted into the seating portion, arranged in a circular shape in the seating portion, and supplied with the nucleic acid separated from the sample. In claim 4,
The PCR tube is a nucleic acid extraction and gene amplification apparatus comprising an extraction tube including a filter therein and an amplification tube coupled to a lower portion of the extraction tube. In claim 4,
The mounting portion, a nucleic acid extraction and gene amplification apparatus comprising a heater located below the PCR tube, controlling the temperature of the PCR tube and amplifying the nucleic acid isolated from the sample. In paragraph 2,
A nucleic acid extraction and gene amplification apparatus including the washing tube inserted into the seating portion and receiving a washing liquid. In clause 7,
The mounting portion, nucleic acid extraction and gene amplification apparatus including a heating tube inserted into the seating portion to evaporate the washing liquid. It is performed in a nucleic acid extraction and gene amplification device consisting of one device,
Supplying a sample containing a nucleic acid to a nucleic acid tube containing a binding buffer solution,
Electrical stimulation is applied to the sample by an electrode located under the nucleic acid tube,
Isolating the nucleic acid from the sample,
The step of lowering the solvent container toward the nucleic acid tube,
Supplying a solvent to the nucleic acid tube by the solvent container,
Binding the nucleic acid and magnetic beads by the binding buffer,
The solvent container rises and the electromagnet bar descends toward the nucleic acid tube,
Holding the nucleic acid to which the electromagnet bar is bound and the magnetic bead to move it to a washing tube containing a washing solution,
Washing the combined nucleic acid and the magnetic beads with the washing solution,
The electromagnet bar descends toward the washing tube and the combined nucleic acid and the magnetic bead are moved to a heating tube,
Evaporating the washing liquid contained in the bound nucleic acid and the magnetic beads,
The electromagnet bar descends toward the heating tube, and the combined nucleic acid and the magnetic bead are moved to a PCR tube containing an elution buffer,
Separating the combined nucleic acid and the magnetic bead,
The electromagnet bar descends toward the PCR tube and the magnetic bead is moved to the storage tube, and
Amplifying the nucleic acid contained in the PCR tube
Including,
The washing tube is located on the counterclockwise side of the nucleic acid tube, the PCR tube is located on the counterclockwise side of the washing tube, and the storage tube is located on the counterclockwise side of the PCR tube,
The solvent container, the pressure bar, and the electromagnet bar are sequentially positioned in a counterclockwise direction, rotate in one direction around a drive shaft, and each independently elevate and descend,
The electromagnet bar is sequentially raised and lowered in the nucleic acid tube, the washing tube, the PCR tube, and the storage tube,
The electromagnet bar rotates in the one direction and is located above the PCR tube and descends into the PCR tube, and the nucleic acid and the magnetic bead are separated by the elution buffer, and
The pressure bar is rotated in the one direction, positioned above the PCR tube, descends into the PCR tube, and a nucleic acid extraction and gene amplification method in which the separated nucleic acid is amplified. In claim 9,
The step of amplifying the nucleic acid contained in the PCR tube,
The step of lowering the pressure bar toward the PCR tube,
The nucleic acid passes through a filter and moves to an amplification tube,
Controlling the temperature of the amplifying tube by a heater, and
The step of amplifying the nucleic acid by the temperature control
Nucleic acid extraction and gene amplification method comprising a.

Images