CN209412202U - A kind of device of the extracting of the subregion in-situ nucleic acid on cell filter membrane - Google Patents
A kind of device of the extracting of the subregion in-situ nucleic acid on cell filter membrane Download PDFInfo
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- CN209412202U CN209412202U CN201820163869.1U CN201820163869U CN209412202U CN 209412202 U CN209412202 U CN 209412202U CN 201820163869 U CN201820163869 U CN 201820163869U CN 209412202 U CN209412202 U CN 209412202U
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- filter membrane
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Abstract
The utility model discloses a kind of on cell filter membrane device that subregion in-situ nucleic acid extracts, including cell filter membrane placement plate, subregion extracting chamber and pressing plate；The cell filter membrane placement plate includes groove, fixing pipe bottom and circular groove, groove and circular groove are arranged in cell filter membrane placement plate upper end, the aperture at the aperture ratio fixing pipe bottom of groove is small, and circular groove is symmetrically arranged with pressing plate for placing subregion extracting chamber, the two sides of subregion extracting chamber.Whole cell filter membrane is divided into several zonules by the utility model, under the action of two pressure plates, forms several relatively independent small chambers, each small chamber can independently carry out in-situ nucleic acid extracting, interfere each other small；Zoning design effectively reduces heteroproteose cell quantity, effectively improves the purity of extract product；And the fixing pipe bottom of pressing plate bottom can easily be fixed on thermostatical instrument, can effectively conduct heat between the device and external environment, and it is practical.
Description
Technical field
It is specifically a kind of for subregion in-situ nucleic acid extracting on cell filter membrane the utility model relates to field of biotechnology
Device.
Background technique
Circulating tumor cell (Circulating tumor cells, CTCs), which refers to, is detached from primary tumo(u)r lesion, invasion
And enter the tumour cell of the circulatory systems such as lymphatic vessel, blood, marrow.CTCs number or parting, characteristic are analyzed, had
Help external early diagnosis, Monitoring of drug resistance, judging prognosis and the survival analysis of tumour, detection tumor recurrence, evaluation drug are treated
Effect is to assist in the treatment of decision and adjustment therapeutic scheme.It is more likely to count in conjunction with CTCs with downstream analysis at present and carries out experiment knot
The comprehensive descision of fruit or the tumor patient state of an illness.It can be respectively in protein level, rna level and DNA level pair after concentration and separation
CTCs cell mass carries out molecule parting and signature analysis.For example, CTCs express spectra is analyzed using RT-PCR, it can also benefit
Mutation, genome of CTCs etc. are analyzed with targeting PCR, DNA sequencing etc..Meanwhile also using unicellular full-length genome
Sequencing, full sequencing of extron group, RNA sequencing.But there is also some problems simultaneously for these CTCs analysis methods, such as use ISET
After method is enriched with CTCs, when carrying out DNA level analysis to CTCs, CTC how is efficiently separated, leucocyte ratio how is effectively reduced
How example, realize the CTCs nucleic acid extraction that can be used for subsequent analysis, how to improve the DNA of tumor cell accounting in the DNA of extracting
Extract that target product purity is low, target cell removing is difficult Deng, the existing device for extracting nucleic acid.
Utility model content
The purpose of this utility model is to provide a kind of on cell filter membrane device that subregion in-situ nucleic acid extracts, with solution
Certainly the problems mentioned above in the background art.
To achieve the above object, the utility model provides the following technical solutions:
A kind of device of the extracting of the subregion in-situ nucleic acid on cell filter membrane, including the extracting of cell filter membrane placement plate, subregion
Chamber and pressing plate；The cell filter membrane placement plate includes groove, fixing pipe bottom and circular groove, and groove and circular groove setting exist
The aperture of cell filter membrane placement plate upper end, the aperture ratio fixing pipe bottom of groove is small, and circular groove extracts chamber for placing subregion,
The two sides of subregion extracting chamber are symmetrically arranged with pressing plate, and described pressing plate one end has a circular trough, and circular trough inner sidewall is equipped with
The groove being arranged on screw thread and cell filter membrane placement plate is connect by screw, and the other end of each pressing plate is connected to subregion extracting
The fixation male arms upper end of chamber.
As a further solution of the present invention: the cell filter membrane placement plate is made of easy heat-conducting.
The fixing pipe bottom is structure as a whole with cell filter membrane placement plate as a further solution of the present invention,.
The subregion extracting chamber is hollow cylindrical structure, subregion extracting as a further solution of the present invention,
Male arms and a positioning male arms are fixed there are two being fixedly connected in the outside lower end of chamber.
Nine are divided by four pieces of demarcation plates on the inside of the subregion extracting chamber as a further solution of the present invention,
A different extracting small chamber.
Compared with prior art, the utility model has the beneficial effects that whole cell filter membrane is divided by the utility model
Several zonules form several relatively independent small chambers under the action of two pressure plates, and each small chamber can independently carry out original
Position nucleic acid extraction, is interfered small each other；Zoning design effectively reduces heteroproteose cell quantity, effectively improves the purity of extract product；
And the fixing pipe bottom of pressing plate bottom can easily be fixed on thermostatical instrument, can effectively be passed between the device and external environment
Heat, it is practical.
Detailed description of the invention
Fig. 1 is the structural schematic diagram for the device that subregion in-situ nucleic acid extracts on cell filter membrane.
Fig. 2 is that subregion extracts the structural schematic diagram of chamber in the device of subregion in-situ nucleic acid extracting on cell filter membrane.
In figure: 1- cell filter membrane placement plate, 2- subregion extracting chamber, 3- pressing plate, 4- groove, 5- fixing pipe bottom, 6- are round
The fixed male arms of groove, 8- demarcation plate, 9-, 10- position male arms.
Specific embodiment
The following will be combined with the drawings in the embodiments of the present invention, carries out the technical scheme in the embodiment of the utility model
Clearly and completely describe, it is clear that the described embodiments are only a part of the embodiments of the utility model, rather than whole
Embodiment.Based on the embodiments of the present invention, those of ordinary skill in the art are without making creative work
Every other embodiment obtained, fall within the protection scope of the utility model.
Fig. 1 ~ 2, a kind of device of the extracting of the subregion in-situ nucleic acid on cell filter membrane, including cell filter membrane is please referred to place
Plate 1, subregion extracting chamber 2 and pressing plate 3；The cell filter membrane placement plate 1 is made of easy heat-conducting, cell filter membrane placement plate
1 includes groove 4, fixing pipe bottom 5 and circular groove 6, and fixing pipe bottom 5 is structure as a whole with cell filter membrane placement plate 1,4 He of groove
Circular groove 6 is arranged in 1 upper end of cell filter membrane placement plate, and the aperture at the aperture ratio fixing pipe bottom 5 of groove 4 is small, and circular groove 6 is used
In placing filter membrane and subregion extracting chamber 2, the subregion extracting chamber 2 is hollow cylindrical structure, and subregion extracts the outside of chamber 2
Lower end is fixedly connected there are two fixed male arms 9 and a positioning male arms 10, and the two sides of subregion extracting chamber 2 are symmetrically arranged with pressing plate
3, described 3 one end of pressing plate has a circular trough, and circular trough inner sidewall is recessed equipped with being arranged on screw thread and cell filter membrane placement plate
Slot 4 is connected by screw, and the other end of each pressing plate 3 is connected to upper end in the fixation male arms 9 of subregion extracting chamber 2；It is described
2 inside of subregion extracting chamber is divided into nine different extracting small chambers by four pieces of demarcation plates 8.
The working principle of the utility model is: the smooth circular groove for being just placed in cell filter membrane placement plate of cell filter membrane to be measured
It is interior, it places subregion and extracts chamber, then one end of 2 block pressur plates is placed in the fixation male arms of subregion extracting chamber, the other end
Groove in thread groove and cell filter membrane placement plate is tightened subregion extracting chamber by screw.By mounted cell
On filter membrane subregion in-situ nucleic acid extraction device by the fixing pipe bottom fixed placement under supporting plate in PCR instrument, in certain to be extracted
The cell pyrolysis liquid of certain volume is added in one specific small chamber, closes PCR instrument slot cover, carries out cell cracking reaction；Cell is split
After the completion of solution, by cell pyrolysis liquid out of each small chamber to be extracted middle taking-up and new cell filter membrane to be extracted is installed can be into
The new cell cracking operation of row.
It is obvious to a person skilled in the art that the present invention is not limited to the details of the above exemplary embodiments, and
And without departing substantially from the spirit or essential attributes of the utility model, it can realize that this is practical new in other specific forms
Type.Therefore, in all respects, the present embodiments are to be considered as illustrative and not restrictive, this is practical new
The range of type is indicated by the appended claims rather than the foregoing description, it is intended that containing for the equivalent requirements of the claims will be fallen in
All changes in justice and range are embraced therein.It should not treat any reference in the claims as limiting
Related claim.
In addition, it should be understood that although this specification is described in terms of embodiments, but not each embodiment is only wrapped
Containing an independent technical solution, this description of the specification is merely for the sake of clarity, and those skilled in the art should
It considers the specification as a whole, the technical solutions in the various embodiments may also be suitably combined, forms those skilled in the art
The other embodiments being understood that.
Claims (5)
1. a kind of device of the extracting of the subregion in-situ nucleic acid on cell filter membrane, including the extracting of cell filter membrane placement plate (1), subregion
Chamber (2) and pressing plate (3)；It is characterized in that, the cell filter membrane placement plate (1) includes groove (4), fixing pipe bottom (5) and circle
Connected in star (6), groove (4) and circular groove (6) are arranged in cell filter membrane placement plate (1) upper end, and the aperture ratio of groove (4) is fixed
The aperture of tube bottom (5) is small, and circular groove (6) extracts the two of chamber (2) for placing filter membrane and subregion extracting chamber (2), subregion
Side is symmetrically arranged with pressing plate (3), and described pressing plate (3) one end has a circular trough, and circular trough inner sidewall is equipped with screw thread and cell
The groove (4) being arranged in filter membrane placement plate is connected by screw, and the other end of each pressing plate (3) is connected to subregion extracting chamber
(2) fixation male arms (9) upper end.
2. the device according to claim 1 for subregion in-situ nucleic acid extracting on cell filter membrane, which is characterized in that described
Cell filter membrane placement plate (1) is made of easy heat-conducting.
3. the device according to claim 1 for subregion in-situ nucleic acid extracting on cell filter membrane, which is characterized in that described
Fixing pipe bottom (5) is structure as a whole with cell filter membrane placement plate (1).
4. the device according to claim 1 for subregion in-situ nucleic acid extracting on cell filter membrane, which is characterized in that described
It is hollow cylindrical structure that subregion, which extracts chamber (2), and the outside lower end of subregion extracting chamber (2) is fixedly connected with that there are two fixed male arms
(9) and a positioning male arms (10).
5. the device according to claim 4 for subregion in-situ nucleic acid extracting on cell filter membrane, which is characterized in that described
Nine different extracting small chambers are divided by four pieces of demarcation plates (8) on the inside of subregion extracting chamber (2).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820163869.1U CN209412202U (en) | 2018-01-31 | 2018-01-31 | A kind of device of the extracting of the subregion in-situ nucleic acid on cell filter membrane |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820163869.1U CN209412202U (en) | 2018-01-31 | 2018-01-31 | A kind of device of the extracting of the subregion in-situ nucleic acid on cell filter membrane |
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| Publication Number | Publication Date |
|---|---|
| CN209412202U true CN209412202U (en) | 2019-09-20 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201820163869.1U Active CN209412202U (en) | 2018-01-31 | 2018-01-31 | A kind of device of the extracting of the subregion in-situ nucleic acid on cell filter membrane |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN209412202U (en) |
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2018
- 2018-01-31 CN CN201820163869.1U patent/CN209412202U/en active Active
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