CN210506380U

CN210506380U - Cell culture device that can be used to adherent cell adds medicine experiment

Info

Publication number: CN210506380U
Application number: CN201921380546.9U
Authority: CN
Inventors: 陈豫钦; 王健; 卢文菊
Original assignee: First Affiliated Hospital of Guangzhou Medical University
Current assignee: First Affiliated Hospital of Guangzhou Medical University
Priority date: 2019-08-23
Filing date: 2019-08-23
Publication date: 2020-05-12
Anticipated expiration: 2029-08-23

Abstract

The utility model discloses a can be used to cell culture device that adherence cell adds medicine experiment, including cell culture container and dismantled and assembled interval structure, the cell culture container includes vessel and top cap, the vessel inner chamber is arranged in and the inner chamber is separated for two at least cell culture districts to interval structure dismantled and assembled. The cell culture device has the advantages of simple structure, convenient application, low cost, easy operation, reliable experiment quality and stable performance, thereby improving the operability and reproducibility of cell medicine adding experiments.

Description

Cell culture device that can be used to adherent cell adds medicine experiment

Technical Field

The utility model relates to a cell culture device especially relates to a cell culture device that can be used to adherence cell and add medicine experiment.

Background

The cell culture in vitro technology has become one of the common experimental methods in current scientific research, and the important progresses of cell cycle and regulation thereof, canceration mechanism, aging, gene expression and regulation and the like are all undiscovered. Wherein the related functional experiments on the isolated cells are the common research means for the pathophysiology research at present. While functional experiments often involve different drugs to stimulate cells, the current drug experiments mostly require the application of multi-well plates, and the cells need to be digested, counted and plated again. The operation steps are various, the experiment period is long, and the accuracy of the experiment result is often influenced by inaccurate counting or uneven operation of the seed plates.

SUMMERY OF THE UTILITY MODEL

The utility model aims at providing a can be used to adherence cell and add cell culture device of medicine experiment. The cell culture device has the advantages of simple structure, convenient application, easy operation, low cost, reliable experiment quality and stable performance, thereby improving the operability and reproducibility of cell dosing experiments.

In order to realize the above object, the utility model provides a cell culture device that can be used to adherent cell adds medicine experiment, including cell culture container and dismantled and assembled interval structure, cell culture container includes vessel and top cap, the vessel inner chamber is arranged in and the inner chamber is separated for two at least cell culture districts to interval structure dismantled and assembled. In the cell culture, firstly, the cell culture is carried out in a cell culture container, after the cells adhere to the wall, a spacing structure is inserted for spacing partition, and then different cell culture areas can be subjected to different medicine adding tests according to the test requirements.

The outer contour of the spacing structure is matched with the inner cavity of the container body, and at least two through holes penetrating through the top surface and the bottom surface of the spacing structure are arranged on the spacing structure and are used as cell spacing holes. The diameters of the through holes of the spacing structure are equal or different. The through hole is a round hole or a square hole or a hole with other shapes.

As another embodiment, the spacing structure includes a top plate and at least two cylinders, the cylinders are vertically fixed to the bottom surface of the top plate, the central axis of each cylinder is perpendicular to the top plate, and through holes are formed in positions of the top plate corresponding to the cylinders. The inner diameters of the cylinders are equal or unequal. The cylinder body can be a cylinder or a square cylinder or other shapes.

The cell culture container and the spacing structure can be prepared from materials such as polystyrene.

Compared with the prior art, use the utility model discloses reduce the experiment step, the experiment quality is reliable, the stable performance to make and improve the maneuverability and the reproducibility that the adherence cell adds the medicine experiment. The utility model discloses simple structure, low cost, can carry out the industrial production to can select different shapes, not equidimension, not area, the different interval structure in permutation and combination's cell interval hole, convenient to use according to the experiment difference.

Drawings

FIG. 1 is a schematic view of the structure of a cell culture apparatus.

Fig. 2 is a schematic structural diagram of a spacer structure according to the first embodiment.

Fig. 3 is a schematic structural view of a spacer structure according to the second embodiment.

Detailed Description

Example one

The cell culture device that can be used to adherent cell that fig. 1-2 shows adds medicine experiment is an embodiment of the utility model, including cell culture container and dismantled and assembled interval structure 2, the cell culture container includes rectangular vessel 1 and top cap 7. The spacing structure 2 is a polyethylene block with an outer contour matched with the inner cavity of the container body 1, and is provided with six through holes 3 with equal diameters and penetrating through the top surface and the bottom surface of the spacing structure.

When in use, the cultured cells are planted in the cell culture container for culture, the top cover 7 is covered, and the cell culture container is placed in 5% CO₂And culturing at 37 deg.C in a cell culture box. When the cells grow to the density that can be used for the experiment by adhering to the wall, take out the cell culture container, open top cap 7, insert in container body 1 to the bottom along the container body 1 inner chamber border of cell culture container with interval structure 2, interval structure 2 is about to divide into the equal region of a plurality of shapes with the cell, forms a plurality of cell culture areas, and the space in every through-hole 3 is a cell culture area. At this time, different drugs or drugs with different concentrations can be added into different areas for experiments, or the mixture can be covered with the top cover 7 and then placed in a cell culture box for long-time experiments.

The cell culture vessel and the spacer 2 may be made of polystyrene or the like.

Example two

As shown in fig. 3, unlike the first embodiment: the spacing structure comprises a top plate 5 and six cylinders 4 with equal inner diameters, the cylinders are vertically fixed on the bottom surface of the top plate 5, and through holes 6 are arranged at the positions of the top plate 5 corresponding to the cylinders 4.

Claims

1. The utility model provides a can be used to cell culture device of adherent cell medicine experiment, characterized by, includes cell culture container and dismantled and assembled interval structure, cell culture container includes vessel and top cap, interval structure is dismantled and assembled to be arranged in the vessel inner chamber and separate the inner chamber for two at least cell culture district.

2. The cell culture device for the adherent cell drug-adding experiment as claimed in claim 1, wherein the outer contour of the spacing structure is adapted to the inner cavity of the container body, and at least two through holes penetrating the top surface and the bottom surface of the spacing structure are formed in the spacing structure.

3. The cell culture apparatus for adherent cell drug addition experiments according to claim 2, wherein the diameters of the through holes of the spacing structure are equal or different.

4. The cell culture device for the adherent cell drug-adding experiment according to claim 2 or 3, wherein the through hole is a round hole or a square hole.

5. The cell culture device of claim 1, wherein the spacer structure comprises a top plate and at least two cylinders, the cylinders are vertically fixed on the bottom surface of the top plate, the central axis of the cylinders is perpendicular to the top plate, and through holes are formed in the top plate at positions corresponding to the cylinders.

6. The cell culture device of claim 5, wherein the inner diameter of the cylinder is equal or different.

7. The cell culture device for the adherent cell drug-adding experiment according to claim 5 or 6, wherein the cylinder body is a cylinder or a square cylinder.

8. The cell culture apparatus of claim 1, wherein the cell culture vessel and the spacer structure are made of polystyrene.