CN111662825A

CN111662825A - Cell culture device

Info

Publication number: CN111662825A
Application number: CN202010556102.7A
Authority: CN
Inventors: 冯莉雅
Original assignee: Longdong University
Current assignee: Longdong University
Priority date: 2020-06-17
Filing date: 2020-06-17
Publication date: 2020-09-15

Abstract

The invention relates to the field of cell culture devices, and particularly discloses a cell culture device which comprises a box body and a plurality of culture dish fixing parts, wherein the culture dish fixing parts are arranged in the box body and used for placing culture dishes, each culture dish fixing part comprises a side wall, an opening is longitudinally arranged in the side wall in a penetrating mode, a through groove is formed in the side wall, an arc-shaped baffle capable of sliding out and covering the opening is arranged in the through groove, a first sliding groove matched with the bottom of the arc-shaped baffle is formed in the bottom surface of each culture dish fixing part and communicated with the through groove, a second sliding groove matched with the top of the arc-shaped baffle is formed in the bottom of the top surface of each culture dish fixing. According to the invention, the cylindrical culture dish fixing part is arranged to accommodate the culture dish, and the culture dish is stacked in layers and cannot fall off due to the movable limit of the arc-shaped baffle plate, so that the space in the box body is fully utilized, the stable placement of the culture dish is ensured, and the cell pollution caused by sliding is avoided.

Description

Cell culture device

Technical Field

The invention relates to the field of cell culture devices, and particularly discloses a cell culture device.

Background

Cell culture (cell culture) refers to a method for simulating in vivo environment (sterility, proper temperature, pH value, certain nutritional conditions and the like) in vitro to enable the cells to survive, grow and reproduce and maintain main structures and functions. Cell culture is also called cell cloning technology, and the formal term in biology is cell culture technology. Cell culture is an essential process for both the whole bioengineering technique and one of the biological cloning techniques, and is itself a large-scale cloning of cells. The cell culture technology can be used for culturing a single cell into simple single cells or rarely differentiated multiple cells in a large scale, which is an indispensable link of the cloning technology. Cell culture techniques are important and commonly used in cell biology research methods, and a large number of cells can be obtained through cell culture, and signal transduction, anabolism, growth and proliferation of cells and the like of the cells can be researched.

The cell culture case is the indispensable carrier of cultured cell, and current cell culture case is only simple box, goes on with the help of the culture dish when cultured cell, in order to practice thrift the space, during large batch culture, and the laboratory staff stacks the culture dish layer upon layer usually to the culture dish often takes place and falls, causes the breakage of cell pollution and culture dish, causes laboratory staff's injury when getting the culture dish even under the condition that laboratory staff is unknown. Accordingly, the present invention provides a cell culture apparatus.

Disclosure of Invention

In view of the above-mentioned technical disadvantages, it is an object of the present invention to provide a cell culture apparatus that solves the problems identified in the background art.

In order to solve the technical problems, the invention adopts the following technical scheme:

the present invention provides a cell culture apparatus, comprising:

a box body;

the culture dish fixing pieces are arranged in the box body and used for placing culture dishes;

the culture dish fixing piece is in a hollow cylindrical shape and comprises a side wall, an opening longitudinally penetrates through the side wall, a through groove is formed in the side wall, and an arc-shaped baffle capable of sliding out and covering the opening is arranged in the through groove; a first sliding groove matched with the bottom of the arc-shaped baffle is formed in the bottom surface of the culture dish fixing piece, and the first sliding groove is communicated with the through groove;

the bottom of the top surface of the culture dish fixing piece is provided with a second sliding groove matched with the top of the arc-shaped baffle, and the second sliding groove is communicated with the through groove.

Preferably, a plurality of partition plates are arranged inside the culture dish fixing member, and the distance between every two partition plates is greater than the height of the culture dish.

Preferably, the bottom in the box body is provided with a plurality of grooves which can be matched with the bottoms of the culture dish fixing pieces to form clamping positions.

Preferably, the end of the bottom of the arc-shaped baffle, which is far away from the through groove, is provided with a groove, and a protrusion matched with the groove is arranged on one side, which is far away from the through groove, of the first sliding groove.

Preferably, the arc-shaped baffle plate is further provided with a first limiting block and a second limiting block, and the first limiting block and the second limiting block are respectively located at the upper end and the lower end of one side of the inside of the through groove.

Preferably, a shifting sheet is fixedly arranged on one side, far away from the through groove, of the outer side wall of the arc-shaped baffle.

Preferably, a plurality of first vent holes are formed in the side wall.

Preferably, the rear wall of the box body is provided with a plurality of second ventilation holes.

Preferably, the culture device for detecting microorganisms provided by the invention further comprises an amplification culture dish capable of being placed in the culture dish fixing member;

the amplification culture dish comprises a dish bottom and a dish cover, and the dish cover can be covered on the dish bottom;

the outer side wall of the dish bottom is provided with an external thread, the inner side wall of the dish cover is provided with an internal thread matched with the external thread, and the cover surface of one side of the dish cover facing downwards is a spherical surface.

Preferably, the distance from the spherical distal end to the upper surface of the capsule is less than 2/3 of the height of the side wall of the capsule.

The invention has the beneficial effects that: this culture apparatus is spacing through setting up the spacing culture dish of culture dish mounting, and it is spacing through the cowl activity, make the range upon range of culture dish layer put also can not take place the culture dish and fall, both make full use of the space in the box, also guaranteed that the culture dish is stably placed, avoid the cell pollution because of the landing leads to, prevent simultaneously to fall to break into pieces because of the culture dish and cause laboratory staff's health injury.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.

FIG. 1 is a schematic structural view of a cell culture apparatus according to an embodiment of the present invention;

FIG. 2 is a schematic view of the structure of the fixing member of the culture dish in FIG. 1;

FIG. 3 is a schematic view of a connection structure of the arc-shaped baffle and the through groove in FIG. 2;

FIG. 4 is a schematic cross-sectional view of FIG. 2;

FIG. 5 is a schematic view of an enlarged culture dish used in conjunction with FIG. 1;

description of reference numerals: the culture dish comprises a box body 1, a culture dish fixing piece 2, a first sliding groove 3, a side wall 4, an arc-shaped baffle 5, a dish bottom 6, a dish cover 7, a bulge 301, a through groove 401, a shifting piece 501, a first limiting block 502 and a second limiting block 503.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "axial", "radial", "circumferential", etc. indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience of describing technical solutions of the present invention and simplifying the description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention.

As shown in fig. 1 to 5, a cell culture apparatus includes:

a box body 1;

the culture dish fixing pieces 2 are arranged in the box body 1 and used for placing culture dishes;

the culture dish fixing part 2 is in a hollow cylindrical shape, the culture dish fixing part 2 comprises a side wall 4, an opening is longitudinally arranged on the side wall 4 in a penetrating mode, a through groove 401 is formed in the side wall 4, and an arc-shaped baffle 5 capable of sliding out and covering the opening is arranged in the through groove 401; a first sliding groove 3 matched with the bottom of the arc baffle 5 is formed in the bottom surface of the culture dish fixing member 2, and the first sliding groove 3 is communicated with the through groove 401;

the bottom of the top surface of the culture dish fixing part 2 is provided with a second sliding groove matched with the top of the arc baffle 5, and the second sliding groove is communicated with the through groove 401.

During the use, stack the culture dish in inside 2 culture dish mountings, then slip cowl 5 for slip cowl 5 blocks the opening on lateral wall 4, prevents that the culture dish landing from breaking into pieces.

In this embodiment, 2 inside baffles, per two of culture dish mounting are equipped with distance between the baffle is greater than the height of culture dish, the baffle separates into a plurality of chambeies that can place the culture dish with culture dish mounting 2, conveniently gets the culture dish of putting different positions alone in the experimenter.

The bottom has been seted up in the box 1 a plurality ofly can with the recess that 2 bottom adaptations of culture dish mounting formed the screens, culture dish mounting 2 with box 1 can be dismantled to be connected and has been realized dismantling the convenience, and the clearance of being convenient for prevents after forming the screens culture dish mounting 2 emptys.

In this embodiment, 5 bottoms of cowl are kept away from the one end that leads to groove 401 is equipped with the recess, in the first spout 3, keep away from lead to groove 401 one side be equipped with the arch 301 of recess adaptation, 5 roll-offs of cowl and just lid close during the opening, arch 301 just with the recess forms the screens for can remain stable when 5 roll-offs of cowl and just lid close during the opening, just can open under the exogenic action, prevent cowl 5 from sliding by oneself.

Still be equipped with first stopper 502 and second stopper 503 on cowl 5, first stopper 502 with second stopper 503 is located respectively lead to the upper and lower both ends of the inside one side of groove 401, first stopper 502 and second stopper 503 are spacing cowl 5 in the inside logical groove 401 of lateral wall 4, prevent that cowl 5 from dropping.

On 5 lateral walls of cowl, keep away from one side of logical groove 401 is fixed and is equipped with plectrum 501, and setting up of plectrum 501 is convenient to slide cowl 5 with the help of external force.

Be equipped with a plurality of first ventilation holes on lateral wall 4, first ventilation hole sets up and makes culture dish mounting 2 only play the fixed action, and environment such as its inside and outside temperature is unanimous basically.

The rear wall of the box body 1 is provided with a plurality of second ventilation holes, and the second ventilation holes are used for adjusting the temperature in the box body.

The invention also comprises an amplification culture dish which can be placed in the culture dish fixing part 2;

the amplification culture dish comprises a dish bottom 6 and a dish cover 7, and the dish cover 7 can be covered on the dish bottom 6;

6 lateral walls at the bottom of the dish are equipped with the external screw thread, 7 inside walls of dish lid be equipped with the internal thread of external screw thread adaptation, just one side capping down of dish lid 7 is the sphere, and the culture dish of enlargiing is used for cultivateing the cell and observes the cell form, enlargies for the sphere through one side capping down of dish lid 7, selects suitable observation height through inside and outside screw thread adaptation knob.

After the dish cover is closed, the distance from the spherical far end to the upper surface of the dish cover 7 is smaller than 2/3 of the height of the side wall of the dish cover 7, so that the spherical far end is prevented from touching cells on the dish bottom 6.

It will be apparent to those skilled in the art that various changes and modifications may be made in the present invention without departing from the spirit and scope of the invention. Thus, if such modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include such modifications and variations.

Claims

1. A cell culture apparatus, comprising:

a box body (1);

the culture dish fixing pieces (2) are arranged in the box body (1) and used for placing culture dishes;

the culture dish fixing part (2) is in a hollow cylindrical shape, the culture dish fixing part (2) comprises a side wall (4), an opening is longitudinally arranged in the side wall (4) in a penetrating mode, a through groove (401) is formed in the side wall (4), and an arc-shaped baffle (5) capable of sliding out and covering the opening is arranged in the through groove (401); a first sliding groove (3) matched with the bottom of the arc-shaped baffle (5) is formed in the bottom surface of the culture dish fixing piece (2), and the first sliding groove (3) is communicated with the through groove (401); the bottom of the top surface of the culture dish fixing piece (2) is provided with a second sliding groove matched with the top of the arc-shaped baffle plate (5), and the second sliding groove is communicated with the through groove (401).

2. The cell culture apparatus according to claim 1, wherein the culture dish fixing member (2) is provided with a plurality of partitions inside, and a distance between every two partitions is larger than a height of the culture dish.

3. The cell culture device according to claim 2, wherein the bottom of the box body (1) is provided with a plurality of grooves which can be matched with the bottom of the culture dish fixing member (2) to form a clamping position.

4. The cell culture device according to claim 1, wherein a groove is formed at the bottom of the arc-shaped baffle (5) at the end far away from the through groove (401), and a protrusion (301) matched with the groove is formed in the first sliding groove (3) at the side far away from the through groove (401).

5. The cell culture device according to claim 1, wherein the arc-shaped baffle (5) is further provided with a first limiting block (502) and a second limiting block (503), and the first limiting block (502) and the second limiting block (503) are respectively located at the upper end and the lower end of one side inside the through groove (401).

6. The cell culture device according to claim 5, wherein a shifting piece (501) is fixedly arranged on the outer side wall of the arc-shaped baffle (5) and on the side far away from the through groove (401).

7. Cell culture device according to claim 1, characterized in that the side wall (4) is provided with a plurality of first ventilation holes.

8. The cell culture apparatus according to claim 1, wherein the case (1) is provided with a plurality of second ventilation holes in a rear wall thereof.

9. The cell culture apparatus according to claim 1, further comprising an enlarged culture dish capable of being placed in the culture dish holder (2);

the amplification culture dish comprises a dish bottom (6) and a dish cover (7), and the dish cover (7) can be covered on the dish bottom (6);

the outer side wall of the dish bottom (6) is provided with an external thread, the inner side wall of the dish cover (7) is provided with an internal thread matched with the external thread, and the downward side cover surface of the dish cover (7) is a spherical surface.

10. The cell culture apparatus of claim 9, wherein the spherical distal end is spaced from the top surface of the dish lid (7) by a distance less than 2/3 the height of the side wall of the dish lid (7).