CN209797990U - Surface area multiplication cell culture dish - Google Patents
Surface area multiplication cell culture dish Download PDFInfo
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- CN209797990U CN209797990U CN201822128664.2U CN201822128664U CN209797990U CN 209797990 U CN209797990 U CN 209797990U CN 201822128664 U CN201822128664 U CN 201822128664U CN 209797990 U CN209797990 U CN 209797990U
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- culture dish
- surface area
- cell culture
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Abstract
The utility model discloses a surface area cell culture dish of multiplying, including the culture dish, culture dish inner wall and/or bottom be equipped with big surface volume. The large surface integration is a honeycomb body or a continuously arranged conical body or a continuously arranged hexagonal cylinder with a hollow interior. The utility model discloses can be under the condition that does not increase culture dish appearance volume and quantity, make full use of culture dish's cubical space, the attached surface area of doubling cell culture effectively and then increase the cell number that can cultivate.
Description
Technical Field
The utility model relates to a medical culture dish, in particular to culture dish that carries out the cultivation to the adherence cell.
Background
cell culture is involved in the production of antibodies, vaccines and viruses. Cells can be largely divided into suspension cells, which are mainly suspended in the culture medium, and adherent cells, which are more prone to adhere to the surface of the culture vessel during growth, depending on their growth state. Adherent cells have certain advantages for production, particularly for products secreted extracellularly, mainly because intact cells still adhere to the surface of the culture vessel, while the products are generally in the culture supernatant and are thus easier to isolate. On the other hand, adherent cells are limited to the surface area of the culture vessel for mass production, and the following technical problems are easily caused particularly in small-scale development and product preparation: 1) the bottom area of the culture dish is limited, the number of cell culture is not suitable to be overlarge, otherwise, the phenomenon of cell lamination is easy to generate, and the cell growth state is not good; 2) if multiple dishes are used, the volume of medium and other ancillary reagents required during cell culture and downstream transfection can be multiplied, cost prohibitive, and require high parallelism in the procedure.
Disclosure of Invention
The present invention aims at overcoming the shortcomings of the background art, and provides a cell culture dish with a novel structure, which can provide more attachment surface area for the culture of adherent cells.
The technical scheme of the utility model is that: a surface area multiplication cell culture dish comprises a culture dish, wherein a large surface area is arranged on the inner wall and/or the bottom of the culture dish.
Preferably, the large surface integration is in the shape of any one or a combination of a honeycomb body, a continuously arranged conical body, a continuously arranged hexagonal cylinder with a hollow interior, and the like.
The utility model has the advantages that: the three-dimensional space of the culture dish can be fully utilized under the condition of not increasing the appearance volume and the number of the culture dish, the attachment surface area of the cell culture is effectively multiplied, and the number of culturable cells is increased. The volume of the culture medium can not be obviously changed in the culture process, the concentration of the transfection reagent can be almost kept consistent by the old system, but the reaction frequency is improved due to the increase of the number of cells, so that the experiment efficiency and the output can be improved.
Drawings
Fig. 1 is a schematic perspective view of embodiment 1 of the present invention;
Fig. 2 is a schematic cross-sectional structure diagram of embodiment 2 of the present invention;
Wherein: 1. petri dish, 2, Large surface area.
Detailed Description
A cell culture dish with multiplied surface area comprises a culture dish, wherein a large surface integration body is arranged on the inner wall and/or the bottom of the culture dish, and the large surface integration body can be integrally formed with the culture dish when the culture dish is opened, and can also be directly bonded in the culture dish.
The cell culture process is similar to the operation of a common culture dish, namely after the cells are counted, the cells are diluted to a proper volume by taking a proper number of cells and then are uniformly added into the culture dish. Special attention is paid to observing the state of cells due to non-planar structures. Cell culture assays can also be performed to determine the growth curve of cells in this new dish, providing reference data for later experiments.
Cell exchange, transfection and supernatant recovery are similar to normal petri dish operation. Due to the non-planar bottom surface, a moderate inclination is required to ensure that the medium can be aspirated or replaced in its entirety.
As shown in FIG. 1, in example 1, a large surface area body (2) is provided on the bottom of a culture dish (1), and the large surface area body is a hexagonal column with hollow interior and arranged continuously.
As shown in FIG. 2, in example 2, a large surface area body (2) is provided on the bottom of a culture dish (1), and the large surface area body is a cone-shaped body arranged in series. Take 10 cm petri dish as an example: the bottom area of the culture dish is about 78cm2(ii) a If the bottom surface is added with the conical bodies with the diameter of 1 mm and the height of 1 mm, about 10000 conical bodies can be added, and the surface area of each conical body is about 1.7mm2The attachable surface area of the modified culture dish is about 170cm2(ii) a If the bottom surface is replaced by a cone with a diameter of 0.5 mm and a height of 1 mm, about 40000 rivets can be added, and the surface area of each rivet is about 0.88mm2The attachable surface area of the modified culture dish is about 354cm2. By analogy, the cell culture table can be multiplied according to the culture dish forming processArea.
Claims (4)
1. A cell culture dish with surface area multiplication comprises a culture dish and is characterized in that a large surface integration is arranged on the inner wall and/or the bottom of the culture dish.
2. The surface area doubling cell culture dish of claim 1, wherein the large surface area is a honeycomb.
3. The surface area doubling cell culture dish of claim 1, wherein the large surface area is a continuous array of conical bodies.
4. The surface area doubling cell culture dish of claim 1, wherein the large surface area is a continuous array of hexagonal pillars with hollow interiors.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201822128664.2U CN209797990U (en) | 2018-12-19 | 2018-12-19 | Surface area multiplication cell culture dish |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201822128664.2U CN209797990U (en) | 2018-12-19 | 2018-12-19 | Surface area multiplication cell culture dish |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN209797990U true CN209797990U (en) | 2019-12-17 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201822128664.2U Active CN209797990U (en) | 2018-12-19 | 2018-12-19 | Surface area multiplication cell culture dish |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN209797990U (en) |
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2018
- 2018-12-19 CN CN201822128664.2U patent/CN209797990U/en active Active
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