CN209307398U - A kind of plant monocyte generation culture bottle - Google Patents

Abstract

The utility model discloses a kind of plant monocytes to generate culture bottle, it mainly include glass body and its inner sleeve, it is characterized in that the glass body is mainly by cylindrical bottle body, it is adhered to the unicellular entrance and feeding port of cylindrical bottle body, pedestal, and mating and right side is formed equipped with externally threaded lid with glass body, the inner sleeve mainly includes four long connecting rods and two clamping rings for being connected to long connecting rod both ends, the cell screen clothes incremented by successively there are three mesh number are installed from right to left, all sieve peripheries are fitted with watertight rubber ring among long connecting rod.The utility model passes through the disposably long-term continuous culture after feeding port is put into disrupting tissue, by the cell screen clothes of three layers of different pore size, it directly can be directly collected into plant monocyte in unicellular outlet after shaking table shake culture, realize primary inoculation, primary culture, the effect once harvested.It greatly reduces pollution probability and also significantly reduces the technical requirements and workload of laboratory technician.

Description

A kind of plant monocyte generation culture bottle

Technical field

The utility model relates to plant cell engineering equipment, specifically in plant cell engineering plant monocyte system Standby culture bottle.

Background technique

Currently, the source of plant monocyte mainly passes through enzymatic isolation method or Mechanical Method or callus in scientific research Revulsion handles explant or callus to obtain a small amount of plant monocyte and a large amount of cell masses, then these are mixed Unicellular and cell mass together carries out Liquid Culture, carries out 50 mesh apertures in superclean bench after cultivating a period of time Cell after sieving is continued Liquid Culture by sieving, and culture a period of time carries out 100 mesh apertures in superclean bench and is sieved, will Cell after sieving continues Liquid Culture, and culture a period of time carries out 150 mesh apertures in superclean bench and is sieved, obtains just It is plant monocyte.

For existing technical method, in above-mentioned technical process operation, be primarily present 3 point defects and insufficient: 1. Repeatedly highly polluting causes this process to fail therebetween for sieving, and polluting in plant monocyte preparation process can not be remedied. 2. more than sieving, labour's consumption is huge during large scale preparation.3. technical requirements of being sieved are excessively high, careless slightly, it is easy It pollutes.

Utility model content

The utility model lies in that providing a kind of slender cell generation culture of the plant for preparing plant monocyte for laboratory Bottle, to solve the disadvantage in above-mentioned background technique and deficiency.

The technical problem solved by the utility model is implemented by the following technical solutions:

A kind of plant monocyte generation culture bottle, mainly includes glass body and inner sleeve, it is characterised in that: described Glass body mainly by cylindrical bottle body, is adhered to the unicellular entrance and feeding port of cylindrical bottle body, is installed on glass body bottom Pedestal, and with glass body mating and right side equipped with externally threaded lid form, the inner sleeve mainly includes four A long connecting rod and two clamping rings for being connected to long connecting rod both ends, among long connecting rod installation from right to left there are three mesh numbers successively Incremental cell screen clothes, all sieve peripheries are fitted with watertight rubber ring.

For the utility model, three cell screen clothes installed from right to left among the long connecting rod are followed successively by 50 mesh cells Sieve, 100 mesh cell screen clothes, 150 mesh cell screen clothes.

For the utility model, spacing is isometric therebetween for the sieve.

For the utility model, it is latter with going out to need casing is put into advance in bottle body and is added fluid nutrient medium before use Bacterium.

For the utility model, the casing is fixed on glass body by the interaction of watertight rubber circle and lid It is interior.

The utility model has the advantages that the utility model is passed through by the disposably long-term continuous culture after feeding port is put into disrupting tissue The cell screen clothes of three layers of different pore size, can directly in unicellular outlet, to be directly collected into plant slender after shaking table shake culture Born of the same parents realize primary inoculation, primary to cultivate, the effect once harvested.It greatly reduces pollution probability and also significantly reduces The technical requirements and workload of laboratory technician.

Detailed description of the invention

Fig. 1 is the overall structure main view of the utility model；

Fig. 2 is the overall structure top view of the utility model；

Fig. 3 is the overall structure right view of the utility model.

Specific embodiment

To specific embodiment of the present utility model, detailed description are as follows with reference to the accompanying drawing:

As illustrated in the accompanying drawings from 1 to 3, a kind of plant monocyte generates culture bottle, includes mainly glass body 2, passes through vitronectin Unicellular outlet 1 and feeding port 12, the pedestal 11 of bottom glass adhesion external screw thread is equipped at and right openings mating with bottle Lid 4.Casing is equipped with inside glass body, casing is by being supporter with four long connecting rods 7, and there are two the installations of long connecting rod both ends Clamping ring 6 for fixing, the place that four long connecting rods are formed inlay three cell screen clothes be respectively from right to left 50 mesh (" mesh ", How many mesh count in 1 square inch of area, the i.e. mesh count of sieve referred to, for example 100 mesh are exactly at 1 square inch Area in have 100 holes) 8,100 mesh cell screen clothes of cell screen clothes, 9,150 mesh cell screen clothes 10, each cell screen clothes periphery Watertight rubber circle 5 is installed.Each main component is illustrated respectively below:

Cylindrical bottle body 2: glass body, left side seal right openings and are equipped with external screw thread 3, and inner wall is the circle of very standard Column.It can ensure that casing watertight rubber circle 5 can be stuck on inner wall.

Inner sleeve: inner sleeve is one of two core technology points of this technology.Inner sleeve is by four and cylinder The identical long connecting rod 7 of bottle body inner wall length, is fixed on the clamping ring 6 at 7 both ends of long connecting rod, and long connecting rod, which is directly inlayed, fixes three The cell screen clothes of a different pore size size are 50 mesh cell screen clothes, 8,100 mesh cell screen clothes, 9,150 mesh cell respectively from right to left Sieve 10, each sieve spacing is identical, and each cell screen clothes circumference is fitted with watertight rubber circle 5.Inner sleeve is being matched It is placed in 2 inside of cylindrical bottle body before fluid nutrient medium processed and tightens lid 4, watertight rubber circle can be stuck in cylindrical bottle body 2, be protected Card removes cell screen clothes exterior domain no leakage, and isometric long connecting rod can resist lid with cylindrical bottle body internal diameter and cylindrical bottle body is left Wall guarantees that inner sleeve will not slide in earthquake culture.Prepared fluid nutrient medium is added after installing inner sleeve, seals It sterilizes together after mouthful.

Operation principle and process:

1. before carrying out plant monocyte culture: the inner sleeve of cylindrical bottle body being taken out, by glass body and internal sleeve Pipe is cleaned, and inner sleeve is put back to cylindrical bottle body after cleaning up, and tightens lid.In feeding port adding liquid culture medium, Feeding port and unicellular outlet are sealed, high pressure sterilization is carried out.

2. being inoculated with after sterilizing: take out the culture bottle equipped with culture medium that has sterilized, in the superclean bench for bacterium of having gone out into Row operation, opens feeding port sealed membrane, and hormone is added, and slight wobble bottle body mixes hormone, is added in feeding port processed Fragment of tissue.Feeding port is sealed.

3. shake culture: the training method used is placed in the fragment of tissue on the downside of feeding port and is cultivating for shake culture Grown in base, it is most of to generate cell mass, during rotating concussion cell mass less than 50 mesh cell screen clothes apertures can By rotation tangential force by 50 mesh cell screen clothes, it is made of into next 100 mesh cell screen clothes and 50 mesh cell screen clothes In cell, continue division growth, it is unicellular to generate part, and most of is still cell mass, the plant monocyte of formation and small Cell mass can by rotation tangential force penetrate 100 mesh cell screen clothes, by 100 mesh cell screen clothes and 150 mesh cell sieves In the cell for netting composition, small cell mass and the unicellular continuation multiple fission of fraction constantly generate newly unicellular and small Cell mass, it is unicellular thin by 150 mesh by rotation tangential force since 150 mesh cell screen clothes only allow unicellular transmission Born of the same parents' sieve, finally in the unicellular cell for concentrating on being made of 150 mesh cell screen clothes and cylindrical bottle body left wall.

4. harvesting unicellular: the culture bottle that finishes of culture is placed in superclean bench sterilize open it is unicellular out The sealed membrane of mouth, is directly poured out cultured unicellular.

Claims (4)

1. It mainly include glass body and its inner sleeve, it is characterised in that: described 1. a kind of plant monocyte generates culture bottle Glass body mainly by cylindrical bottle body, is adhered to the unicellular entrance and feeding port of cylindrical bottle body, is installed on glass body bottom Pedestal, and with glass body mating and right side equipped with externally threaded lid form, the inner sleeve mainly includes four A long connecting rod and two clamping rings for being connected to long connecting rod both ends, among long connecting rod installation from right to left there are three mesh numbers successively Incremental cell screen clothes, all sieve peripheries are fitted with watertight rubber ring.
2. 2. a kind of plant monocyte according to claim 1 generates culture bottle, which is characterized in that the sieve therebetween between Away from isometric.
3. 3. a kind of plant monocyte according to claim 1 generates culture bottle, which is characterized in that the casing passes through only The interaction of water rubber ring and lid is fixed in glass body.
4. 4. a kind of plant monocyte according to claim 1 generates culture bottle, which is characterized in that among the long connecting rod from Three cell screen clothes of right-to-left installation are followed successively by 50 mesh cell screen clothes, 100 mesh cell screen clothes, 150 mesh cell screen clothes.