CN205052414U - Aseptic hydroponic device of plant - Google Patents
Aseptic hydroponic device of plant Download PDFInfo
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- CN205052414U CN205052414U CN201520816548.3U CN201520816548U CN205052414U CN 205052414 U CN205052414 U CN 205052414U CN 201520816548 U CN201520816548 U CN 201520816548U CN 205052414 U CN205052414 U CN 205052414U
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- filter screen
- aseptic
- diameter
- glass
- hydroponic device
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
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Abstract
The utility model discloses an aseptic hydroponic device of plant, include glass cup (1) and fix in at least one filter screen (2) of its inside bottom be provided with in filter screen (2) and train matrix (3), the open -top of glass cup (1) adopts sealing membrane (4) and seals be provided with water planting liquid (5) in glass cup (1), the liquid level of water planting liquid (5) is less than train the upper surface of matrix (3). The utility model discloses aseptic hydroponic device of plant can handle the aseptic water planting of carrying out high efficiency to A variety of plants in the back through heat sterilization.
Description
Technical field
The utility model relates to a kind of plant cultivation device, is specifically related to a Plants sterile water training device.
Background technology
In the processes such as plant vegetative propagation, genetic transformation, usually need to cut portion of tissue (explant) from positions such as plant leaf blades carry out tissue cultures, the explant obtained from natural environment needs the tissue cultures that just can carry out next step after strict sterilization, this disinfecting process usually has certain toxic action to explant and sterilization may not thoroughly, thus causes the problems such as the low and pollution of explant survival rate.In order to solve the problem, usually first cultivate germ-free plant seedling, then cutting explant from aseptic seedling.Common aseptic seedlings of plants implantation methods is the solid culture medium of preparation containing agar or plant gel etc., plants on solid culture medium after being sterilized by plant seed.
Also there are some defects in the method that solid culture medium is cultivated germ-free plant seedling, as the plants such as clover exist root development slowly on solid culture medium, growth of seedling speed is slower.Because medium Middle nutrition element consumes and the accumulation of toxic metabolic products after cultivating for a long time in addition, Aseptic Seedling Growth is suppressed even dead.The solid culture medium now more renewed is a kind of solution, usually needs aseptic seedling to extract from old solid culture medium to move in new solid culture medium, and this processing ease damages and inconvenient operation plant roots.
Utility model content
The technical problems to be solved in the utility model is to provide a kind of aseptic hydroponic device of plant that can realize the simple fast Aseptic seedling culture of multiple applicable hydroponic plant.
One Plants sterile water training device, comprise at least one filter screen of glass and fixing bottom therein, culture matrix is provided with in described filter screen, the open top of described glass adopts sealed membrane sealing, water planting liquid is provided with in described glass, the liquid level of described water planting liquid is lower than the upper surface of described culture matrix, and described water planting liquid is Hogland nutrient solution or MS culture fluid.
The aseptic hydroponic device of plant described in the utility model, wherein, the bottom centre position of the inside of described glass is provided with fixed leg, the bottom of described filter screen is provided with the fixing hole matched with described fixed leg, described fixing hole is fixed on the bottom of described glass through described fixed leg, described filter screen is stainless steel cylindrical filter screen, and the top diameter of described filter screen is greater than base diameter.
The aseptic hydroponic device of plant described in the utility model, wherein, described filter screen employing thickness is 0.5mm, hole density is that 10 object stainless steel filtering nets are made, and the bottom external diameter of described filter screen is 40mm, and top outer diameter is 43mm, is highly 50mm; The diameter of described fixing hole is 10mm.
The aseptic hydroponic device of plant described in the utility model, wherein, described filter screen is one.
The aseptic hydroponic device of plant described in the utility model, wherein, described filter screen is multiple, multiple described filter screen is added to design height up and down, the described filter screen of bottommost is fixed on the bottom of described glass, in described filter screen topmost, be provided with described culture matrix, the height of the overlapping portion between adjacent two described filter screens is 30mm.
The aseptic hydroponic device of plant described in the utility model, wherein, the bottom of the sidewall of described glass is provided with changes liquid mouth, described in change liquid mouth and adopt the sealing of cone rubber plug.
The aseptic hydroponic device of plant described in the utility model, wherein, the described liquid mouth that changes is cylindrical tube, length is 5mm, and internal diameter is 8mm, external diameter is 10mm, and its one end is connected with the bottom of described glass, the other end adopts described cone rubber plug to seal, the length of described cone rubber plug is 10mm, and one end diameter is 5mm, and other end diameter is 10mm.
The aseptic hydroponic device of plant described in the utility model, wherein, described culture matrix is the quartz sand of particle diameter 3 ~ 5mm, and the liquid level of described water planting liquid is lower than the upper surface 2 ~ 5mm of described culture matrix.
The aseptic hydroponic device of plant described in the utility model, wherein, described glass is drum-shaped, and the thickness of sidewall and bottom is 3mm, internal diameter is 94mm, and external diameter is 100mm, is highly 200 ~ 300mm, described fixed leg is cylindrical glass post, and diameter is 8mm, is highly 30mm.
The aseptic hydroponic device difference from prior art of the utility model plant is:
The aseptic hydroponic device of the utility model plant can by carrying out sterile water training after high-temperature sterilization process; Quartz sand to be convenient to the fixing of plant seedlings and root growth as culture matrix is held using stainless steel filtering net; Liquid-changing hole is arranged at cup bottom, is convenient to water planting fluid exchange when long-time cultivation and not easily pollutes; The aseptic water planting that this hydroponic system can carry out rapidly and efficiently to various plants.
The method tool using the aseptic hydroponic device of the utility model plant to carry out the aseptic water planting of plant has the following advantages:
The plant seed of sterilization treatment or germ-free plant seedling is selected to cultivate in this device, compared with conventional open ciltivating process, adopt the utility model device can carry out the aseptic culture of plant seedlings, a large amount of aseptic explant can be provided for Plant Tissue Breeding and genetic transformation etc.; Mill water culture nutrient solution can for plant provides various nutritive elements needed for good growth, aseptic Rooted Cuttings to be cut after portion of tissue can rapid regeneration, wound is easy infection not, constantly can provide the aseptic explant needed for tissue cultures and genetic transformation; Under the gnotobasis that this device provides, plant seedling growth speed is fast, does not affect by extraneous bacterium, virus, fungi.
The quartz sand culture matrix that the particle selected is larger and the growth of stainless steel filtering net to plant roots have good adaptability.Conventional ciltivating process uses thinner quartz sand, sponge etc. as fixing matrix usually, in these matrix, plant root growth is limited, and observe, collect root system of plant inconvenience, in contrast to this, this utility model device can make root system of plant well-grown in thicker quartz sand hole, be convenient to the collection of root system of plant, root system of plant also stretches out by metal screen mesh, is convenient to root system and observes.
The utility model device is applicable to height lower than the plant of 30cm and the aseptic water planting of seedling, the utility model device is simple to operate, plant seed after applicable plant aseptic seedlings or sterilization is put in super-clean bench the quartz sand surface of this hydroponic device inside, with the sealed membrane of band filter disc, glass rim of a cup is sealed, be then placed in climatic cabinate and carry out water planting.As need long-term cultivation be carried out, bottom of cup perforate can be opened old water planting liquid is released, then add new water planting liquid with after rubber stopper seal from rim of a cup, thus carry out the replacing of water planting liquid.The replacement operation of this water planting liquid is easy.
With carry out compared with aseptic culture in Conventional solid agar medium, this device is adopted to carry out the plant seedlings root system development of aseptic water planting good, well developed root system, seedling is healthy and strong, cauline leaf is sturdy, cradle reviviscence good, solve that plantling poor growth in Conventional solid agar medium, root system are short and small, side root rare, cradle rear regeneration slowly and medium the problem such as not easily to change.
Below in conjunction with accompanying drawing, the aseptic hydroponic device of plant of the present utility model is described further.
Accompanying drawing explanation
Fig. 1 is the structural representation of the glass of the aseptic hydroponic device of the utility model plant;
Fig. 2 is the structural representation of the filter screen of the aseptic hydroponic device of the utility model plant;
Fig. 3 is the structural representation of the aseptic hydroponic device of plant in the utility model embodiment 1;
Fig. 4 is the cross-sectional view of Fig. 3;
Fig. 5 is the structural representation of the aseptic hydroponic device of plant in the utility model embodiment 2;
Fig. 6 is the cross-sectional view of Fig. 5.
Embodiment
Embodiment 1
As shown in Figure 1 to 4, one Plants sterile water training device, comprise at least one filter screen 2 of glass 1 and fixing bottom therein, culture matrix 3 is provided with in filter screen 2, the open top of glass 1 adopts sealed membrane 4 to seal, in glass 1, be provided with water planting liquid 5, the liquid level of water planting liquid 5 is lower than the upper surface 2 ~ 5mm of culture matrix 3.
The bottom centre position of the inside of glass 1 is provided with fixed leg 6, the bottom of filter screen 2 is provided with the fixing hole 7 matched with fixed leg 6, fixing hole 7 is fixed on the bottom of glass 1 through fixed leg 6, and filter screen 2 is stainless steel cylindrical filter screen, and the top diameter of filter screen 2 is greater than base diameter.
Filter screen 2 adopts that thickness is 0.5mm, hole density is that 10 object stainless steel filtering nets are made, and the bottom external diameter of filter screen 2 is 40mm, and top outer diameter is 43mm, is highly 50mm; The diameter of fixing hole 6 is 10mm.
Filter screen 2 is one, the bottom of the sidewall of glass 1 is provided with changes liquid mouth 8, changing liquid mouth 8 adopts cone rubber plug 9 to seal, and change liquid mouth 8 for cylindrical tube, length is 5mm, internal diameter is 8mm, external diameter is 10mm, its one end is connected with the bottom of glass 1, and the other end adopts cone rubber plug 8 to seal, and the length of cone rubber plug 8 is 10mm, one end diameter is 5mm, and other end diameter is 10mm.Culture matrix 3 is the quartz sand of particle diameter 3 ~ 5mm, and water planting liquid 5 is Hogland nutrient solution or MS culture fluid.
Glass 1 is drum-shaped, and the thickness of sidewall and bottom is 3mm, and internal diameter is 94mm, and external diameter is 100mm, is highly 200 ~ 300mm, and fixed leg 6 is cylindrical glass post, and diameter is 8mm, is highly 30mm.
Embodiment 2
As shown in Figure 1, Figure 2, shown in Fig. 5 and Fig. 6, one Plants sterile water training device, comprise at least two filter screens 2 of glass 1 and fixing bottom therein, culture matrix 3 is provided with in filter screen 2, the open top of glass 1 adopts sealed membrane 4 to seal, in glass 1, be provided with water planting liquid 5, the liquid level of water planting liquid 5 is lower than the upper surface 2 ~ 5mm of culture matrix 3.
The bottom centre position of the inside of glass 1 is provided with fixed leg 6, the bottom of filter screen 2 is provided with the fixing hole 7 matched with fixed leg 6, fixing hole 7 is fixed on the bottom of glass 1 through fixed leg 6, and filter screen 2 is stainless steel cylindrical filter screen, and the top diameter of filter screen 2 is greater than base diameter.Filter screen 2 adopts that thickness is 0.5mm, hole density is that 10 object stainless steel filtering nets are made, and the bottom external diameter of filter screen 2 is 40mm, and top outer diameter is 43mm, is highly 50mm; The diameter of fixing hole 6 is 10mm.Filter screen 2 is multiple, and multiple filter screen about 2 is added to design height, and the filter screen 2 of bottommost is fixed on the bottom of glass 1, and in filter screen 2 topmost, be provided with culture matrix 3, the height of the overlapping portion between adjacent two filter screens 2 is 30mm.
The bottom of the sidewall of glass 1 is provided with changes liquid mouth 8, changes liquid mouth 8 and adopts cone rubber plug 9 to seal.Change liquid mouth 8 for cylindrical tube, length is 5mm, and internal diameter is 8mm, external diameter is 10mm, its one end is connected with the bottom of glass 1, and the other end adopts cone rubber plug 8 to seal, and the length of cone rubber plug 8 is 10mm, one end diameter is 5mm, and other end diameter is 10mm.Culture matrix 3 is the quartz sand of particle diameter 3 ~ 5mm, and water planting liquid 5 is Hogland nutrient solution or MS culture fluid.Glass 1 is drum-shaped, and the thickness of sidewall and bottom is 3mm, and internal diameter is 94mm, and external diameter is 100mm, is highly 200 ~ 300mm, and fixed leg 6 is cylindrical glass post, and diameter is 8mm, is highly 30mm.
Adopt embodiment 1 or the aseptic hydroponic device of the plant described in embodiment 2 to carry out the method that aseptic clover seedling is cultivated and explant cuts, comprise the steps:
(A), by the filter screen 2 holding full quartz sand combine with glass 1, with sealed membrane 4, rim of a cup is sealed, use cone rubber plug 9 bottom opening to be sealed, at 121 DEG C, put into super-clean bench after moist heat sterilization 30min after combination for subsequent use; Preparation Hogland nutrient solution or MS culture fluid, at 121 DEG C, to put into super-clean bench for subsequent use for moist heat sterilization 30min.
(B), the alfalfa seed of screening full seed, use 75% alcohol disinfecting 15min, then rinse three times with sterile purified water, often all over 1min.Open the sealed membrane 4 of the aseptic hydroponic device of above-mentioned plant, alfalfa seed 1 ~ 2 is put into the quartz sand surface of filter screen 2, Hogland nutrient solution through high-temperature sterilization or MS culture fluid is added from glass 1 madial wall, liquid level is lower than quartz sand surface height 2 ~ 5mm, then use sealed membrane 4 by the sealing suitable for reading of glass 1, above-mentioned sowing operates in superclean bench and completes.Aseptic hydroponic device is after planting placed in climatic cabinate cultivate.
(C), within every 6 ~ 10 days, in super-clean bench, a water planting liquid is changed, water planting liquid 5 is flowed out the taking-up of glass 1 bottom pyramidal rubber stopper 9 through changing liquid mouth when changing Hogland nutrient solution or MS culture fluid, after Hogland nutrient solution or MS culture fluid all flow out, reuse cone rubber plug 9 sealing change liquid mouth, open upper seal film 4 and add new Hogland nutrient solution or MS culture fluid along madial wall, newly add the liquid level of water planting liquid lower than culture matrix 3 surface 10 ~ 20mm.
(D), the clover seedling of aseptic water planting about 30 days can cut the sterile tissue such as blade, root and carries out tissue cultures and genetic transformation as explant in superclean bench.
Above-described embodiment is only be described preferred embodiment of the present utility model; not scope of the present utility model is limited; under the prerequisite not departing from the utility model design spirit; the various distortion that those of ordinary skill in the art make the technical solution of the utility model and improvement, all should fall in protection domain that the utility model claims determine.
Claims (9)
1. a Plants sterile water training device, it is characterized in that: at least one filter screen (2) comprising glass (1) and fixing bottom therein, culture matrix (3) is provided with in described filter screen (2), the open top of described glass (1) adopts sealed membrane (4) sealing, water planting liquid (5) is provided with in described glass (1), the liquid level of described water planting liquid (5) is lower than the upper surface of described culture matrix (3), and described water planting liquid (5) is Hogland nutrient solution or MS culture fluid.
2. the aseptic hydroponic device of plant according to claim 1, it is characterized in that: the bottom centre position of the inside of described glass (1) is provided with fixed leg (6), the bottom of described filter screen (2) is provided with the fixing hole (7) matched with described fixed leg (6), described fixing hole (7) is fixed on the bottom of described glass (1) through described fixed leg (6), described filter screen (2) is stainless steel cylindrical filter screen, and the top diameter of described filter screen (2) is greater than base diameter.
3. the aseptic hydroponic device of plant according to claim 2, it is characterized in that: described filter screen (2) employing thickness is 0.5mm, hole density is that 10 object stainless steel filtering nets are made, the bottom external diameter of described filter screen (2) is 40mm, top outer diameter is 43mm, is highly 50mm; The diameter of described fixing hole (6) is 10mm.
4. the aseptic hydroponic device of plant according to claim 2, is characterized in that: described filter screen (2) is one.
5. the aseptic hydroponic device of plant according to claim 2, it is characterized in that: described filter screen (2) is for multiple, multiple described filter screen (2) is added to design height up and down, the described filter screen (2) of bottommost is fixed on the bottom of described glass (1), in described filter screen (2) topmost, be provided with described culture matrix (3), the height of the overlapping portion between adjacent two described filter screens (2) is 30mm.
6. the aseptic hydroponic device of plant according to claim 1, it is characterized in that: the bottom of the sidewall of described glass (1) is provided with changes liquid mouth (8), described in change liquid mouth (8) adopt cone rubber plug (9) sealing.
7. the aseptic hydroponic device of plant according to claim 6, it is characterized in that: described in change liquid mouth (8) be cylindrical tube, length is 5mm, internal diameter is 8mm, external diameter is 10mm, its one end is connected with the bottom of described glass (1), and the other end adopts described cone rubber plug (8) to seal, and the length of described cone rubber plug (8) is 10mm, one end diameter is 5mm, and other end diameter is 10mm.
8. the aseptic hydroponic device of plant according to claim 1, it is characterized in that: the quartz sand that described culture matrix (3) is particle diameter 3 ~ 5mm, the liquid level of described water planting liquid (5) is lower than the upper surface 2 ~ 5mm of described culture matrix (3).
9. the aseptic hydroponic device of plant according to claim 2, it is characterized in that: described glass (1) is drum-shaped, the thickness of sidewall and bottom is 3mm, internal diameter is 94mm, external diameter is 100mm, is highly 200 ~ 300mm, and described fixed leg (6) is cylindrical glass post, diameter is 8mm, is highly 30mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520816548.3U CN205052414U (en) | 2015-10-20 | 2015-10-20 | Aseptic hydroponic device of plant |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520816548.3U CN205052414U (en) | 2015-10-20 | 2015-10-20 | Aseptic hydroponic device of plant |
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| CN205052414U true CN205052414U (en) | 2016-03-02 |
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| CN201520816548.3U Expired - Fee Related CN205052414U (en) | 2015-10-20 | 2015-10-20 | Aseptic hydroponic device of plant |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105210837A (en) * | 2015-10-20 | 2016-01-06 | 中国农业科学院北京畜牧兽医研究所 | The aseptic hydroponic device of plant is used to carry out the method for rhizobium dross effect assessment |
| CN107637491A (en) * | 2017-09-22 | 2018-01-30 | 浙江省林业科学研究院 | Suitable for physiology and the arabidopsis cultural method and culture apparatus special of genetic research |
-
2015
- 2015-10-20 CN CN201520816548.3U patent/CN205052414U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105210837A (en) * | 2015-10-20 | 2016-01-06 | 中国农业科学院北京畜牧兽医研究所 | The aseptic hydroponic device of plant is used to carry out the method for rhizobium dross effect assessment |
| CN105210837B (en) * | 2015-10-20 | 2018-04-06 | 中国农业科学院北京畜牧兽医研究所 | The method for carrying out rhizobium dross effect assessment using the sterile hydroponic device of plant |
| CN107637491A (en) * | 2017-09-22 | 2018-01-30 | 浙江省林业科学研究院 | Suitable for physiology and the arabidopsis cultural method and culture apparatus special of genetic research |
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| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160302 Termination date: 20201020 |
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| CF01 | Termination of patent right due to non-payment of annual fee |