CN208980729U - A kind of small-sized continuous anaerobic fermentation device - Google Patents

Abstract

The utility model provides a kind of small-sized continuous anaerobic fermentation device, it is related to anaerobic fermentation equipment technical field, air inlet pipe is placed in mouth cylindrical part through the bottle stopper of anaerobism fluid infusion bottle bottle mouth position, one end of liquid supplementation pipe is placed in mouth cylindrical part through the bottle stopper of anaerobic fermentation bottle bottle mouth position, and the other end is placed in bottom of bottle portion through the bottle stopper of anaerobism fluid infusion bottle bottle mouth position；One end of exhaust pipe is placed in mouth cylindrical part through the bottle stopper of anaerobic fermentation bottle bottle mouth position, and the other end is connected with exhaust non-return structure；Outlet tube one end is placed in bottom of bottle portion through the bottle stopper of anaerobic fermentation bottle bottle mouth position, and the other end is in communication with the outside.Inert gas is passed through the increase fluid infusion pressure in the bottle in anaerobism fluid infusion bottle by the utility model, so that anaerobic culture medium is entered in fermentation flask by liquid supplementation pipe, realize continuous fluid infusion, fermentation material is directly contacted with aerobic air when fluid infusion being avoided to operate, conducive to the lasting progress of anaerobic fermentation, and prevent suck-back phenomenon by the way that paraffin layer is arranged.

Description

A kind of small-sized continuous anaerobic fermentation device

Technical field

The utility model relates to anaerobic fermentation equipment technical fields, and in particular to one kind is directed to laboratory room small-sized anaerobic fermentation The device of Shi Jinhang continuous anaerobic fermentation.

Background technique

The environment and human body of human lives, which are inherently survived, the anaerobe of huge number, the relationship of they and the mankind Closely.Large industrialized anaerobic ferment devices such as methane-generating pit is more mature at this stage at present, but can be used for testing indoor small and detest Aerobe fermentation device is fewer.Common laboratory carries out the Intermittent fermentation mode that anaerobic fermentation mostly uses all-in and all-out, this hair Ferment mode is that directly strain is seeded in anaerobism bottle to carry out closed fermentation, and ingress of air, whole device do not begin fermentation process Maintain the state of anaerobic eventually, but this device, there is also defect, the gas generated in fermentation process can not be expelled directly out, such as Fruit carries out the discharge of exhaust gas using gas-guide tube mode is increased, then closed anaerobic fermentation environment can be easy to cause to be destroyed, can only Carry out Intermittent fermentation, it is difficult to the operation such as carry out fluid infusion and change liquid, and open fermentation flask and carry out fluid infusion and then easily cause external micro- life Object pollution and the destruction of anaerobic environment；Closed environment causes sampling difficult, and the metabolism that can not be monitored in time in fermentation process becomes Change.

Utility model content

The utility model provides a kind of small-sized continuous precisely in order to avoid above-mentioned existing deficiencies in the technology Anaerobic ferment devices solve progress anaerobic fermentation in laboratory existing in the prior art and mostly use Intermittent fermentation device, difficult To carry out continuous anaerobic fermentation.

The present invention adopts the following technical scheme that a kind of small-sized continuous anaerobic fermentation device in order to solve the technical problem, including detests Aerobe fermentation bottle, anaerobism fluid infusion bottle, air inlet pipe, liquid supplementation pipe, exhaust pipe, outlet tube and exhaust non-return structure, the air inlet pipe run through The bottle stopper of the anaerobism fluid infusion bottle bottle mouth position is placed in mouth cylindrical part, and the anaerobic fermentation bottle bottleneck is run through in one end of the liquid supplementation pipe The bottle stopper at place is placed in mouth cylindrical part, and the other end is placed in bottom of bottle portion through the bottle stopper of the anaerobism fluid infusion bottle bottle mouth position；The exhaust One end of pipe is placed in mouth cylindrical part through the bottle stopper of the anaerobic fermentation bottle bottle mouth position, and the other end is connected with exhaust non-return structure；Institute The bottle stopper that outlet tube one end is stated through the anaerobic fermentation bottle bottle mouth position is placed in bottom of bottle portion, and the other end is in communication with the outside.

Further, bottom is provided with stirrer in the anaerobic fermentation bottle.

Further, the anaerobic fermentation bottle and anaerobism fluid infusion bottle are cylindrical glass bottle.

Further, valve is respectively arranged on the air inlet pipe, liquid supplementation pipe, exhaust pipe and outlet tube.

Further, the air inlet pipe, liquid supplementation pipe, exhaust pipe and outlet tube are glass tube.

Further, it is provided with paraffin layer in the exhaust non-return structure, and is located at the above position setting exhaust of paraffin layer Hole.

The utility model provides a kind of small-sized continuous anaerobic fermentation device, has the advantages that

1, anaerobism fluid infusion bottle provided by the utility model, the inert gases such as nitrogen enter anaerobism by air inlet pipe and mend In liquid bottle, as the fluid infusion pressure in the bottle increases, anaerobic culture medium is entered in fermentation flask by liquid supplementation pipe, realizes continuous fluid infusion, keep away Exempt from directly to contact when fluid infusion operation with aerobic air, is conducive to the lasting progress of anaerobic fermentation.Anaerobic fermentation bottle normal fermentation produces Raw gas is by exhaust pipe through exhaust non-return mechanism discharge, the presence of paraffin layer, it is therefore prevented that suck-back phenomenon generates.

2, device provided by the utility model can use negative pressure device in fermentation process at outlet tube, by fermentation liquid from Extraction carries out metabolic analysis in anaerobic fermentation bottle, can also increase anaerobic fermentation bottle internal pressure for fermentation liquid by closing exhaust valve It extrudes, does not influence the anaerobic state in anaerobic fermentation bottle.

3, the utility model provides device, can be placed in constant temperature blender with magnetic force, water-bath, ferment in constant incubator Operation, easy to use, operation miniaturization.

Detailed description of the invention

Fig. 1 is the structural schematic diagram of the utility model；

In figure:

1, anaerobic fermentation bottle；2, anaerobism fluid infusion bottle；3, air inlet pipe；4, liquid supplementation pipe；5, exhaust pipe；6, outlet tube；7, it is vented Non-return structure；8, bottle stopper；9, stirrer；31, valve；71, paraffin layer；72, gas vent.

Specific embodiment

It is practical new below in conjunction with this to keep the objectives, technical solutions, and advantages of the embodiments of the present invention clearer Type embodiment, the technical scheme in the utility model embodiment is clearly and completely described, it is clear that described implementation Example is the utility model a part of the embodiment, instead of all the embodiments.Based on the embodiments of the present invention, this field Those of ordinary skill's every other embodiment obtained without creative efforts, belongs to the utility model The range of protection.

As shown in Figure 1, the utility model embodiment provides a kind of small-sized continuous anaerobic fermentation device, including anaerobic fermentation bottle 1, anaerobism fluid infusion bottle 2, air inlet pipe 3, liquid supplementation pipe 4, exhaust pipe 5, outlet tube 6 and exhaust non-return structure 7, air inlet pipe 3 run through anaerobism The bottle stopper 7 of 2 bottle mouth position of fluid infusion bottle is placed in mouth cylindrical part, and the bottle stopper 8 of 1 bottle mouth position of anaerobic fermentation bottle is run through simultaneously in one end of liquid supplementation pipe 4 It is placed in mouth cylindrical part, the other end is placed in bottom of bottle portion through the bottle stopper 8 of 2 bottle mouth position of anaerobism fluid infusion bottle；Anaerobism is run through in one end of exhaust pipe 5 The bottle stopper 8 of 1 bottle mouth position of fermentation flask is placed in mouth cylindrical part, and the other end is connected with exhaust non-return structure 8；Anaerobism is run through in 6 one end of outlet tube The bottle stopper 8 of 1 bottle mouth position of fermentation flask is placed in bottom of bottle portion, and the other end is in communication with the outside.

Preferably, bottom is provided with stirrer 9 in anaerobic fermentation bottle 1.

Preferably, anaerobic fermentation bottle 1 and anaerobism fluid infusion bottle 2 are cylindrical glass bottle.

Preferably, valve 31 is respectively arranged on air inlet pipe 3, liquid supplementation pipe 4, exhaust pipe 5 and outlet tube 6.

Preferably, air inlet pipe 3, liquid supplementation pipe 4, exhaust pipe 5 and outlet tube 6 are glass tube.

Preferably, it is vented in non-return structure 7 and is provided with paraffin layer 71, and be located at 71 or more position of paraffin layer and gas vent is set 72。

Whole device is in use, the inert gases such as nitrogen are entered in anaerobism fluid infusion bottle by air inlet pipe, with fluid infusion bottle Interior pressure increases, and anaerobic culture medium is entered in fermentation flask by liquid supplementation pipe, realizes continuous fluid infusion, avoid fluid infusion when operating with it is aerobic Air directly contacts.The gas that anaerobic fermentation bottle normal fermentation generates is by exhaust pipe through exhaust non-return mechanism discharge, paraffin layer Presence, it is therefore prevented that suck-back phenomenon generate.Fermentation liquid can be sent out from anaerobism using negative pressure device at outlet tube in fermentation process Extraction carries out metabolic analysis in ferment bottle, can also be extruded fermentation liquid by closing exhaust valve increase anaerobic fermentation bottle internal pressure.

The device can be placed in constant temperature blender with magnetic force, water-bath, ferment in constant incubator, applied widely, behaviour It minimizes.

It should be noted that, in this document, relational terms such as first and second and the like are used merely to a reality Body or operation are distinguished with another entity or operation, are deposited without necessarily requiring or implying between these entities or operation In any actual relationship or order or sequence.Moreover, the terms "include", "comprise" or its any other variant are intended to Non-exclusive inclusion, so that the process, method, article or equipment including a series of elements is not only wanted including those Element, but also including other elements that are not explicitly listed, or further include for this process, method, article or equipment Intrinsic element.In the absence of more restrictions, the element limited by sentence "including a ...", it is not excluded that There is also other identical elements in process, method, article or equipment including the element.

Above embodiments are only to illustrate the technical solution of the utility model, rather than its limitations；Although referring to aforementioned reality Example is applied the utility model is described in detail, those skilled in the art should understand that: it still can be to preceding Technical solution documented by each embodiment is stated to modify or equivalent replacement of some of the technical features；And these It modifies or replaces, the spirit and model of various embodiments of the utility model technical solution that it does not separate the essence of the corresponding technical solution It encloses.

Claims (6)

1. a kind of small-sized continuous anaerobic fermentation device, including anaerobic fermentation bottle (1), anaerobism fluid infusion bottle (2), air inlet pipe (3), fluid infusion Manage (4), exhaust pipe (5), outlet tube (6) and exhaust non-return structure (7), which is characterized in that the air inlet pipe (3) is detested through described The bottle stopper (8) of oxygen fluid infusion bottle (2) bottle mouth position is placed in mouth cylindrical part, and the anaerobic fermentation bottle is run through in one end of the liquid supplementation pipe (4) (1) bottle stopper (8) of bottle mouth position is placed in mouth cylindrical part, and the other end is placed in through the bottle stopper (8) of anaerobism fluid infusion bottle (2) bottle mouth position Bottom of bottle portion；One end of the exhaust pipe (5) is placed in mouth cylindrical part through the bottle stopper (8) of anaerobic fermentation bottle (1) bottle mouth position, another End is connected with exhaust non-return structure (7)；The bottle stopper (8) of anaerobic fermentation bottle (1) bottle mouth position is run through in described outlet tube (6) one end It is placed in bottom of bottle portion, the other end is in communication with the outside.

2. small-sized continuous anaerobic fermentation device according to claim 1, it is characterized in that: the anaerobic fermentation bottle (1) interior bottom Portion is provided with stirrer (9).

3. small-sized continuous anaerobic fermentation device according to claim 1, it is characterized in that: the anaerobic fermentation bottle (1) and detesting Oxygen fluid infusion bottle (2) is cylindrical glass bottle.

4. small-sized continuous anaerobic fermentation device according to claim 1, it is characterized in that: the air inlet pipe (3), liquid supplementation pipe (4), valve (31) are respectively arranged on exhaust pipe (5) and outlet tube (6).

5. small-sized continuous anaerobic fermentation device according to claim 1, it is characterized in that: the air inlet pipe (3), liquid supplementation pipe (4), exhaust pipe (5) and outlet tube (6) are glass tube.

6. small-sized continuous anaerobic fermentation device according to claim 1, it is characterized in that: in the exhaust non-return structure (7) It is provided with paraffin layer (71), and is located at paraffin layer (71) above position setting gas vent (72).