CN219136798U

CN219136798U - Microorganism adaptability evolution instrument

Info

Publication number: CN219136798U
Application number: CN202223606264.0U
Authority: CN
Inventors: 王立言; 段保峰; 李红歌
Original assignee: Luoyang Tmaxtree Biotechnology Co ltd
Current assignee: Luoyang Tmaxtree Biotechnology Co ltd
Priority date: 2022-12-30
Filing date: 2022-12-30
Publication date: 2023-06-06
Anticipated expiration: 2032-12-30

Abstract

The utility model belongs to the technical field of microorganism culture, in particular to a microorganism adaptive evolutionary instrument, which is characterized by comprising a plurality of culture units with the same structure and 1 total control system in parallel connection, wherein each culture unit comprises a first power module and a culture module; the first power module is used for conveying a microorganism sample, a culture medium and a reagent; the culture module comprises a second power module, a culture bin, a culture pipeline, a buffer bottle, a temperature control device and an oxygen partial pressure controller, and the second power module drives the microorganism sample to circularly move in the culture pipeline; the control system is used for controlling the running state of each culture unit and processing the data measured by the detection module. The microbial adaptive evolutionary apparatus is microbial domestication equipment developed based on a high gas permeability micro pipeline and a microfluidic technology, and has the functions of microbial culture, automatic passage, automatic chemical factor gradient addition, real-time detection, oxygen partial pressure control and the like.

Description

Microorganism adaptability evolution instrument

Technical Field

The utility model relates to the technical field of microorganism culture, in particular to a microorganism adaptive evolutionary instrument.

Background

The adaptive laboratory evolution is a method for artificially simulating variation and selection process in natural evolution under laboratory conditions, realizing directional evolution of microorganisms by means of artificial selection pressure, and screening individuals with excellent properties from an evolved population; the traditional microbial adaptive evolution instrument is mainly based on culture methods such as shake flasks, porous plates and the like, is realized through operations such as timing passage, chemical factor addition, detection and the like, is severely dependent on manpower, complex and complex in process and high in pollution risk, and particularly in a plurality of parallel experiments, the consistency of experimental operations is difficult to control, so that the problem is solved.

Disclosure of Invention

(one) solving the technical problems

Aiming at the defects of the prior art, the utility model provides a microorganism adaptive evolutionary apparatus, which solves the problems in the background art.

(II) technical scheme

The utility model adopts the following technical scheme for realizing the purposes:

the microbial adaptive evolutionary instrument comprises a plurality of culture units with the same structure and 1 total control system which are connected in parallel, wherein each culture unit comprises a first power module and a culture module; the first power module is used for conveying a microorganism sample, a culture medium and a reagent; the culture module comprises a second power module, a culture bin, a culture pipeline, a buffer bottle, a temperature control device and an oxygen partial pressure controller, wherein the second power module drives a microorganism sample to circularly move in the culture pipeline; the control system is electrically connected with each culture unit, and is used for controlling the running state of each culture unit and processing the data measured by the detection module.

Further, the number of the culture units is 1 to 10.

Further, the first power module and the second power module comprise a plurality of pumps, the types of the pumps comprise one or more of peristaltic pumps, syringe pumps and embolic pumps, and the number of the pumps in the first power module is 1-6.

Further, the culture bin is a closed space, and the buffer bottle and the culture pipeline are arranged inside the culture bin.

Further, the buffer bottle body is provided with scale marks, the buffer bottle is provided with a bottle cap, the bottle cap and the bottle body are tightly nested in the bottle body, the relative position of the bottle cap is adjustable, and the bottle cap is provided with a guide pipe.

Further, the buffer bottle is provided with a biological filter membrane.

Further, the temperature control device adjusts the temperature in the culture bin, and the oxygen partial pressure controller adjusts the gas content in the culture bin.

Further, the culture pipeline is coiled and placed in the culture bin, is a micro pipeline with high gas permeability and is connected with the second power module.

Further, the detection module comprises any one or more of an ion detection component, a photoelectric detection component and an enzyme membrane detection component.

Further, the culture units can work independently or simultaneously, and the working state can be set by a control system.

(III) beneficial effects

Compared with the prior art, the utility model provides a microorganism adaptive evolutionary apparatus, which comprises the following components

The beneficial effects are that:

the microbial adaptive evolutionary instrument is a microbial domestication device developed based on a high-gas-permeability micro pipeline and a microfluidic technology, and has the functions of microbial culture, automatic passage, automatic chemical factor gradient addition, real-time detection, oxygen partial pressure control and the like;

the device takes the high gas permeability micro-pipeline as a reaction container, can quickly exchange mass energy with the surrounding environment, has good permeation to various gases (oxygen, nitrogen, carbon dioxide and the like), and fully meets the gas exchange requirement in the fermentation process; by means of the turbulence of the pipeline flow itself, mixing of the culture can be achieved, and the damage to biological cells can be greatly reduced due to the small shearing force.

Through the oxygen partial pressure control technology, the surrounding gas environment of the reactor can be flexibly regulated and controlled, so that the culture and adaptive evolution of various microorganisms are applied.

Drawings

FIG. 1 is a schematic flow diagram of the present utility model;

FIG. 2 is a schematic diagram of the structure of the culture unit of the present utility model;

in the figure: 1. a culturing unit; 2. a first power module; 3. a culture module; 4. a detection module; 5. a control system; 6. a second power module; 7. a culture pipeline; 8. a buffer bottle; 9. a temperature control device; 10. an oxygen partial pressure controller; 11. a conduit; 12. a biological filter membrane; 13. a bottle cap; 14. a culture bin; 15. and (3) a bracket.

Detailed Description

Embodiments of the present utility model are described in detail below, examples of which are illustrated in the accompanying drawings, wherein the same or similar reference numerals refer to the same or similar elements or elements having the same or similar functions throughout. The embodiments described below by referring to the drawings are exemplary only for explaining the present utility model and are not to be construed as limiting the present utility model.

In the description of the present utility model, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings are merely for convenience in describing the present utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present utility model. Furthermore, the terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying a relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include one or more of the described features. In the description of the present utility model, the meaning of "a plurality" is two or more, unless explicitly defined otherwise.

In the description of the present utility model, it should be noted that, unless explicitly specified and limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be either fixedly connected, detachably connected, or integrally connected, for example; can be mechanically connected, electrically connected or can be communicated with each other; can be directly connected or indirectly connected through an intermediate medium, and can be communicated with the inside of two elements or the interaction relationship of the two elements. The specific meaning of the above terms in the present utility model can be understood by those of ordinary skill in the art according to the specific circumstances.

In the description of the present utility model, unless explicitly stated and limited otherwise, a first feature "above" or "below" a second feature may include both the first and second features being in direct contact, or may include both the first and second features not being in direct contact but being in contact by another feature therebetween. Moreover, a first feature being "above," "over" and "on" a second feature includes the first feature being directly above and obliquely above the second feature, or simply indicating that the first feature is higher in level than the second feature. The first feature being "under", "below" and "beneath" the second feature includes the first feature being directly under and obliquely below the second feature, or simply means that the first feature is less level than the second feature.

The following disclosure provides many different embodiments, or examples, for implementing different features of the utility model. In order to simplify the present disclosure, components and arrangements of specific examples are described below. They are, of course, merely examples and are not intended to limit the utility model. Furthermore, the present utility model may repeat reference numerals and/or letters in the various examples, which are for the purpose of brevity and clarity, and which do not themselves indicate the relationship between the various embodiments and/or arrangements discussed. In addition, the present utility model provides examples of various specific processes and materials, but one of ordinary skill in the art will recognize the application of other processes and/or the use of other materials.

Examples

As shown in fig. 1 and 2, a microorganism adaptive evolutionary apparatus according to an embodiment of the present utility model includes a plurality of

culture units

1 and 1 total control system 5, each of which has the same structure, wherein the culture unit 1 includes a first power module 2 and a culture module 3; the first power module 2 is used for conveying a microorganism sample, a culture medium and a reagent; the culture module 3 comprises a second power module 6, a culture bin 14, a culture pipeline 7, a buffer bottle 8, a temperature control device 9 and an oxygen partial pressure controller 10, wherein the second power module 6 drives a microorganism sample to circularly move in the culture pipeline 7; the control system 5 is used for controlling the running state of each culture unit 1 and processing the data measured by the detection module 4.

The working principle is as follows: the parameters are set through the control system 5, the first power module 2 extracts a fixed amount of prepared bacterial liquid, a culture medium and a reagent, the bacterial liquid, the culture medium and the reagent are conveyed into the buffer bottle to be mixed, the bacterial liquid is extracted into the culture pipeline 7 from the buffer bottle 8 through the second power module 6, the circulating motion culture is carried out under the action of the second power module 6, the temperature of the culture bin can be controlled through the temperature control device 9 in the culture process, the gas content in the culture bin is controlled through the oxygen partial pressure controller 10, and the bacterial liquid in the culture pipeline 7 can be rapidly in mass-energy exchange with the outside due to the fact that the culture pipeline 7 is a high-ventilation micro pipeline, the gas exchange requirement in the culture process is fully met, and the bacterial liquid can grow in a preset environment. When subculture is needed, the bacteria liquid in the culture pipeline 7 is conveyed into the buffer bottle by the second power module 6, the first power module 2 is used for pumping out the redundant bacteria liquid, the required inoculation amount is left, the inoculation amount can be preset according to the scale of the bottle body of the buffer bottle, a proper amount of culture medium is supplemented by the first power module 2, and the mixed bacteria liquid is conveyed into the culture pipeline by the second power module 6 for continuous culture. Every passage, the required reagent can be added through the combination of the power module 2, so that different experimental requirements can be met.

In some embodiments, the number of the culture units 1 is 1-10, the culture units 1 can work independently or simultaneously, and the working state can be set by the control system 5; the first power module 2 and the second power module 6 comprise a plurality of pumps, the types of the pumps comprise one or more of peristaltic pumps, injection pumps and embolism pumps, the number of the pumps in the first power module 3 is 1-6, the types of the pumps preferably comprise peristaltic pumps, and the peristaltic pumps can accurately control the quantity of bacteria liquid and reagents to be conveyed, and are simple in structure and low in cost.

In some embodiments, the culture bin 14 is a closed space, the culture pipeline 7 is coiled and placed inside the culture bin 14, and the culture pipeline 7 is a micro-pipeline with high gas permeability, so that the mass energy exchange with the external environment in the microorganism growth process can be satisfied.

The culture pipeline 7 is connected with the second power module 6, and the second power module 6 can culture bacterial liquid in the culture pipeline 7 in a circulating motion under the drive of the control system 5.

The bottle 8 is arranged in the culture bin 14, the bottle body of the buffer bottle 8 is provided with scale marks, the buffer bottle 8 is provided with a bottle cap 13, the bottle cap 13 is tightly nested in the bottle body, the relative position of the bottle cap 13 is adjustable, and the inoculation amount of a microorganism sample in passage can be controlled through the adjustment of the relative position of the bottle cap 13 and the buffer bottle 8.

The upper surface of the bottle cap 13 is provided with 5 guide pipes 11, one of the guide pipes is relatively longer, 4 shorter guide pipes are used for adding bacteria liquid, culture medium and reagent into the buffer bottle 8, the longer guide pipes are used as channels, samples can be taken from the buffer bottle 8 for detection in the culture process, and solutions in the bottle can be transported in the passage process.

The culture pipeline 7 is connected with the bottle body 8, so that the buffer bottle 8 and the culture pipeline 7 form a complete loop; the buffer bottle 8 is provided with a biological filter membrane 12 for balancing the air pressure inside and outside the buffer bottle 8.

The rigid support 15 is arranged below the culture pipeline 7, and plays a role in supporting the culture pipeline 7 and preventing the culture pipeline 7 from being deformed and broken due to dead weight.

The temperature control device 9 and the oxygen partial pressure controller 10 are arranged at the bottom of the culture bin 14 and are respectively used for adjusting the temperature and the gas content in the culture bin 14.

In some embodiments, the detection module 4 includes any one or more of an ion detection assembly, a photoelectric detection assembly and an enzyme membrane detection assembly, after the culturing is completed, the bacterial liquid passes through the detection module 4 under the drive of the second power module 6, various parameters of the bacterial liquid are detected, and the control system 5 reads the recorded parameters for analysis by an experimenter.

Finally, it should be noted that: the foregoing description is only a preferred embodiment of the present utility model, and the present utility model is not limited thereto, but it is to be understood that modifications and equivalents of some of the technical features described in the foregoing embodiments may be made by those skilled in the art, although the present utility model has been described in detail with reference to the foregoing embodiments. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present utility model should be included in the protection scope of the present utility model.

Claims

1. A microbial adaptation evolutionary apparatus, characterized in that: the device comprises a plurality of culture units with the same structure and 1 total control system which are connected in parallel, wherein the culture units comprise a first power module and a culture module; the first power module is used for conveying a microorganism sample, a culture medium and a reagent; the culture module comprises a second power module, a culture bin, a culture pipeline, a buffer bottle, a temperature control device and an oxygen partial pressure controller, wherein the second power module drives a microorganism sample to circularly move in the culture pipeline; the control system is electrically connected with each culture unit, and is used for controlling the running state of each culture unit and processing the data measured by the detection module.

2. The microbial adaptive evolutionary apparatus of claim 1, wherein: the number of the culture units is 1-10.

3. The microbial adaptive evolutionary apparatus of claim 1, wherein: the first power module and the second power module comprise a plurality of pumps, the types of the pumps comprise one or more of peristaltic pumps, injection pumps and embolic pumps, and the number of the pumps in the first power module is 1-6.

4. The microbial adaptive evolutionary apparatus of claim 1, wherein: the culture bin is a closed space, and the buffer bottle and the culture pipeline are arranged inside the culture bin.

5. The apparatus of claim 4, wherein the apparatus comprises: the buffer bottle body is provided with scale marks, the buffer bottle is provided with a bottle cap, the bottle cap is tightly nested in the bottle body, the relative position of the bottle cap is adjustable, and the bottle cap is provided with a guide pipe.

6. The apparatus of claim 5, wherein the apparatus comprises: the buffer bottle is provided with a biological filter membrane.

7. The microbial adaptive evolutionary apparatus of claim 1, wherein: the temperature control device adjusts the temperature in the culture bin, and the oxygen partial pressure controller adjusts the gas content in the culture bin.

8. The microbial adaptive evolutionary apparatus of claim 1, wherein: the culture pipeline is coiled and placed in the culture bin, is a high-gas permeability micro pipeline and is connected with the second power module.

9. The microbial adaptive evolutionary apparatus of claim 1, wherein: the detection module comprises any one or more of an ion detection component, a photoelectric detection component and an enzyme membrane detection component.

10. The microbial adaptive evolutionary apparatus of claim 1, wherein: the culture units can work independently or simultaneously, and the working state can be set by a control system.