CN216808800U - Tandem type microorganism reaction equipment - Google Patents
Tandem type microorganism reaction equipment Download PDFInfo
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- CN216808800U CN216808800U CN202220006958.1U CN202220006958U CN216808800U CN 216808800 U CN216808800 U CN 216808800U CN 202220006958 U CN202220006958 U CN 202220006958U CN 216808800 U CN216808800 U CN 216808800U
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Abstract
The utility model provides a series microbial reaction device which comprises a plurality of microbial culture bottles connected in series in sequence, wherein each microbial culture bottle is internally provided with a carrier suitable for growth of microbes; a liquid supply bottle connected with the upstream of the plurality of microorganism culture bottles and used for continuously supplying culture liquid to the carriers in the microorganism culture bottles; and the waste liquid collecting bottle is connected with the downstream of the plurality of microorganism culture bottles and is used for collecting waste liquid discharged after the microorganisms in each microorganism culture bottle grow. The series-connection type microorganism reaction equipment adopts a continuous flow enrichment culture mode, and can discharge waste liquid in time while continuously supplementing culture solution into each microorganism culture bottle, thereby optimizing the growth metabolic environment of microorganisms and finally effectively simulating the growth environment of K-type microorganisms in a natural ecological system.
Description
Technical Field
The utility model relates to the technical field of microorganism culture apparatus, in particular to a series-connection type microorganism reaction device.
Background
Microorganisms in nature are classified into "R-tactic bacteria (hereinafter, abbreviated as R-bacteria)" and "K-tactic bacteria (hereinafter, abbreviated as K-bacteria)" according to survival strategies. The R bacteria have higher growth rate under the condition that the substrate is not limited in the natural environment, so the R bacteria are easier to culture under the laboratory condition through the conventional technology; while K bacteria are competitive in natural environment under conditions of limited substrate, they grow slowly or are difficult to culture under laboratory conditions by conventional techniques (i.e., traditional agar plate culture or broth batch culture).
Under laboratory conditions, more than 99% of microorganisms in the natural environment cannot be cultured by conventional techniques, and are referred to as "uncultured microorganisms". Most of the uncultured microorganisms are K bacteria, and the culture technology for K bacteria is not mature.
In carrying out the concept of the present invention, the inventors found that at least the following problems exist in the conventional techniques: 1) compared with the natural environment condition, the artificial culture medium is over eutrophic, and the artificial culture medium with rich nutrition can inhibit the growth of a plurality of microorganisms; 2) it is difficult to perform the cultivation for a long time; 3) the artificial culture medium can generate various toxic substances in the preparation and culture processes, and the conventional technology is difficult to eliminate the adverse effects of the toxic substances on target microorganisms; 4) the artificial culture medium lacks unknown growth factors or signal molecules in the natural environment, and some dependent microorganisms cannot perform normal growth metabolism under the condition of lacking the substances. The above problems make K bacteria difficult to culture under laboratory conditions.
In the course of implementing the concept of the present invention, the inventors found that although the continuous flow bioreactor technology originally applied to sewage treatment has been successfully applied to the enrichment and culture of K bacteria of several specific metabolic pathways, several continuous flow bioreactors applied to the enrichment and culture of microorganisms in the prior art have large volumes, complicated structures, complicated operations and maintenance and are difficult to perform effective overall sterilization, and thus are difficult to popularize and apply in common microbiological laboratories.
SUMMERY OF THE UTILITY MODEL
The utility model provides a series-connection type microbial reaction device which is used for effectively simulating the growth environment of K-type microbes in a natural ecosystem, and comprises a plurality of microbial culture bottles, liquid supply bottles and waste liquid collecting bottles, wherein:
a plurality of microorganism culture bottles are sequentially connected in series, and a carrier suitable for microorganism growth is arranged in each microorganism culture bottle;
the liquid supply bottle is used for continuously supplying culture liquid to the carriers in each microorganism culture bottle and is connected with the upstream of the plurality of microorganism culture bottles;
the waste liquid collecting bottle is used for collecting waste liquid discharged after the microorganism in each microorganism culture bottle grows and is connected with the downstream of the plurality of microorganism culture bottles.
In a specific implementation, the tandem type microorganism reaction equipment further comprises: first air feed mechanism, second air feed mechanism and waste gas recovery mechanism, wherein:
the first gas supply mechanism is communicated with the liquid supply bottle through a gas guide tube and is used for conveying inert gas to a position below the liquid level of the culture solution in the liquid supply bottle so as to remove dissolved oxygen in the culture solution;
the second gas supply mechanism is communicated with a bottom gas inlet interface of the first microorganism culture mechanism in a group through a gas guide tube and is used for supplying a gas medium to the carrier;
the waste gas recovery mechanism is communicated with the neck air outlet interface of the waste liquid collecting mechanism through an air duct and is used for collecting waste gas discharged through the carrier.
In a specific implementation, the carrier comprises at least one gel microsphere bag, and the gel microsphere bag is suspended inside the microorganism culture bottle.
In specific implementation, the gel micro-ball bag comprises a non-woven bag and a plurality of gel micro-balls, and the gel micro-balls are filled in the non-woven bag.
In specific implementation, the gel microspheres are agarose microspheres or calcium alginate microspheres.
In specific implementation, the outside of the gel micro-bag is further wrapped with a support net, and the gel micro-bag is hung inside the microorganism culture bottle through a hook of the support net.
In specific implementation, the liquid supply bottle is a two-way bottle, the two-way bottle comprises a two-way bottle body and a bottle cap part, and the bottle cap part comprises a threaded bottle cap with a hole at the top, a two-way interface and a silica gel sealing ring.
In the concrete implementation, the microorganism blake bottle reaches the waste liquid receiving flask is the cross-port bottle, the cross-port bottle includes cross-port bottle body and bottle cap portion, wherein:
the bottle cap part comprises a threaded bottle cap with a hole at the top, a two-way interface and a silica gel sealing ring;
the bottom and the neck of the four-way bottle body are respectively provided with a threaded cap interface for connecting the air duct.
In specific implementation, the microorganism culture bottles or the liquid supply bottles and the microorganism culture bottles are communicated through liquid guide pipe liquid paths, an upstream interface of the liquid guide pipe is communicated with a liquid outlet interface at the bottle cap of the liquid supply bottle or the microorganism culture bottle, a downstream interface of the liquid guide pipe is communicated with a liquid inlet interface at the bottle cap of the microorganism culture bottle or the waste liquid collecting bottle, and the inner end of each liquid outlet interface is connected with the liquid guide pipe and extends into the bottom of the liquid supply bottle, the microorganism culture bottle or the waste liquid collecting bottle.
In specific implementation, the microorganism culture bottles or the waste liquid collecting bottle and the microorganism culture bottles are communicated through air ducts, the upstream interface of each air duct is communicated with the screw cap interface at the bottleneck of the microorganism culture bottle, and the downstream interface of each air duct is communicated with the screw cap interface at the bottom of the microorganism culture bottle or the downstream interface of each air duct is communicated with the screw cap interface at the bottom of the waste liquid collecting bottle.
The utility model provides a series-connection type microbial reaction device which comprises a plurality of microbial culture bottles connected in series in sequence, wherein a carrier suitable for microbial growth is arranged in each microbial culture bottle; a liquid supply bottle connected to the upstream of the plurality of microorganism culture bottles for continuously supplying a culture liquid to the carriers in the microorganism culture bottles; and the waste liquid collecting bottle is connected with the downstream of the plurality of microorganism culture bottles and is used for collecting waste liquid discharged after the microorganisms in each microorganism culture bottle grow. The series-connection type microorganism reaction equipment adopts a continuous flow enrichment culture mode, and can discharge waste liquid in time while continuously supplementing culture solution to each microorganism culture bottle, thereby optimizing the growth metabolic environment of microorganisms and finally effectively simulating the growth environment of K-type microorganisms in a natural ecological system.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the embodiments or the technical solutions in the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and other drawings can be obtained by those skilled in the art without creative efforts. In the drawings:
FIG. 1 is a schematic view showing the construction of a tandem type microorganism reaction apparatus according to an embodiment of the present invention;
FIG. 2 is a schematic structural view of a tandem type microorganism reaction apparatus having a supply mechanism and an exhaust gas collecting mechanism according to an embodiment of the present invention;
FIG. 3 is a schematic diagram showing the detailed structure of a carrier according to an embodiment of the present invention;
fig. 4 is a schematic view showing a detailed structure of a bottle cap section according to an embodiment of the present invention.
Description of the symbols:
1 microorganism culture bottle 2 liquid supply bottle
3 waste liquid collecting bottle 4 first air feed mechanism
5 second air supply mechanism 6 waste gas recovery mechanism
11 screw thread bottle cap
12 two-way interface 13 silica gel gasket
14 air inlet interface and 15 air outlet interface
C Carrier D gel microsphere
B non-woven bag S supporting net
W plumb N waste liquid discharge port
L culture solution Pl0-P19 catheter
P20-P26 tracheal tube M1 feeding pump
M2 air pump F air filter
V1 valve-closing V2 check valve
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the embodiments of the present invention are further described in detail below with reference to the accompanying drawings. The exemplary embodiments and descriptions of the present invention are provided to explain the present invention, but not to limit the present invention.
As shown in fig. 1, the present invention provides a tandem type microorganism reaction apparatus for effectively simulating a growth environment of K-type microorganisms in a natural ecosystem, the tandem type microorganism reaction apparatus comprising a plurality of microorganism culture bottles 1, a liquid supply bottle 2, and a waste liquid collection bottle, wherein:
a plurality of microorganism culture bottles 1 are sequentially connected in series, and a carrier 3 suitable for microorganism growth is arranged in each microorganism culture bottle 1;
the liquid supply bottle 2 is used for continuously supplying culture liquid to the carriers 3 in the microorganism culture bottles 1 and is connected with the upstream of the microorganism culture bottles 1;
the waste liquid collecting bottle is used for collecting waste liquid discharged after the microorganism in each microorganism culture bottle 1 grows and is connected with the downstream of the microorganism culture bottles 1.
In a specific implementation, as shown in fig. 2, the tandem-type microbial reaction apparatus further includes: first air feed mechanism 4, second air feed mechanism 5 and waste gas recovery mechanism 6, wherein:
the first gas supply mechanism 4 is communicated with the liquid supply bottle 2 through a gas guide tube and is used for conveying inert gas to a position below the liquid level of the culture solution in the liquid supply bottle 2 so as to remove dissolved oxygen in the culture solution L;
the second gas supply mechanism 5 is communicated with a bottom gas inlet interface of the first microorganism culture mechanism in a group through a gas guide tube and is used for supplying a gas medium to the carrier 3;
the waste gas recovery mechanism 6 is communicated with the neck gas outlet interface of the waste liquid collection mechanism through a gas guide tube and is used for collecting waste gas discharged through the carrier 3.
In particular embodiments, the carrier 3 can be provided in a variety of embodiments. For example, as shown in fig. 3, the carrier 3 may include at least one gel microsphere pocket suspended inside the microorganism culture flask 1. Furthermore, the gel micro-ball bag comprises a non-woven bag B and a plurality of gel micro-balls D, and the gel micro-balls D are filled in the non-woven bag B. Furthermore, the gel microspheres D can be agarose microspheres or calcium alginate microspheres.
In specific implementation, the outside of the gel micro-bag is further wrapped with a support net S, and the gel micro-bag is hung inside the microorganism culture bottle 1 through a hook of the support net S.
In specific implementation, the liquid supply bottle 2 can be selected and arranged in various embodiments. For example, as shown in fig. 4, the liquid supply bottle 2 may be a two-way bottle, the two-way bottle includes a two-way bottle body and a bottle cap portion, and the bottle cap portion includes a threaded bottle cap 11 having an opening at a top portion, a two-way port 12, and a silicone gasket 13.
In specific implementation, the microorganism culture bottle 1 can be selected and arranged in various embodiments. For example, as shown in fig. 4, the microorganism culture bottle 1 and the waste liquid collecting bottle 3 are all four-way bottles, and each four-way bottle includes a four-way bottle body and a bottle cap portion, wherein:
the bottle cap part comprises a threaded bottle cap 11 with an opening at the top, a two-way port 12 and a silica gel gasket 13;
the bottom and the neck of the four-way bottle body are respectively provided with a threaded cap interface for connecting the air duct.
In specific implementation, as shown in fig. 1, fig. 2, fig. 3 and fig. 4, the microorganism culture bottles 1 or the liquid supply bottle 2 and the microorganism culture bottles 1 are communicated through a liquid guide tube liquid path, an upstream interface of the liquid guide tube is communicated with a liquid outlet interface at the bottle cap of the liquid supply bottle 2 or the microorganism culture bottle 1, a downstream interface of the liquid guide tube is communicated with a liquid inlet interface at the bottle cap of the microorganism culture bottle 1 or the waste liquid collection bottle, and an inner end of each liquid outlet interface is connected with the liquid guide tube and extends into the bottom of the liquid supply bottle 2, the microorganism culture bottle 1 or the waste liquid collection bottle.
In specific implementation, as shown in fig. 1, fig. 2, fig. 3 and fig. 4, the microorganism culture bottles 1 or the waste liquid collection bottle and the microorganism culture bottles 1 are communicated through an air duct, an upstream interface of the air duct is communicated with a screw cap interface at the bottle neck of the microorganism culture bottle 1, and a downstream interface of the air duct is communicated with a screw cap interface at the bottle bottom of the microorganism culture bottle 1 or a downstream interface of the air duct is communicated with a screw cap interface at the bottle bottom of the waste liquid collection bottle.
In summary, the tandem type microorganism reaction apparatus provided by the present invention comprises a plurality of microorganism culture bottles 1 connected in series in sequence, wherein each microorganism culture bottle 1 is provided with a carrier 3 suitable for microorganism growth; a liquid supply bottle 2 connected to the upstream of the plurality of microorganism culture bottles 1 for continuously supplying a culture liquid to the carriers 3 in the microorganism culture bottles 1; and a waste liquid collecting bottle connected to the downstream of the plurality of microorganism culture bottles 1, for collecting waste liquid discharged after the microorganisms in each microorganism culture bottle 1 grow. The series-connection type microorganism reaction equipment adopts a continuous flow enrichment culture mode, and can discharge waste liquid in time while continuously supplementing culture solution to each microorganism culture bottle 1, thereby optimizing the growth metabolic environment of microorganisms and finally effectively simulating the growth environment of K-type microorganisms in a natural ecological system.
DETAILED DESCRIPTION OF EMBODIMENT (S) OF INVENTION
As shown in fig. 1, the microorganism reaction apparatus includes a set of three microorganism culture bottles 1, a liquid supply bottle 2 disposed upstream of the microorganism culture bottles, and a waste liquid collection bottle 3 disposed downstream.
Specifically, 1 to 3 carriers C for growth of microorganisms are provided in the microorganism culture bottle 1 in a manner of hanging by hooks.
In the embodiment of the utility model, the carrier C is the non-woven bag gel microspheres, and the gel microspheres provide enlarged surface area for the attachment of microorganisms, increase the retention time of the microorganisms and facilitate further strain separation. The gel microsphere material in the carrier C can comprise low-melting-point agarose, calcium alginate and the like. The microorganisms may include methanobacteria, methylotrophic bacteria, nitrobacteria, denitrifying bacteria, and metabolic pluripotency bacteria.
In a specific embodiment, the liquid supply bottle 2 is disposed upstream of each microorganism culture bottle 1, and the liquid supply bottle 2 is used for continuously supplying the culture solution L to the microorganisms in the carrier C of the microorganism culture bottle 1.
In specific implementation, the liquid supply bottle 2 adopts a two-way interface reagent feeding bottle. The two-way connector 12 is arranged at the threaded bottle cap 11, the two-way connector 12 comprises stainless steel, and the threaded bottle cap 11 comprises PVC plastic. One of the two-way connectors 12 is a culture solution outlet 121, the inner end of the culture solution outlet 121 extends into the bottom of a culture solution L in the liquid supply bottle 2 through a liquid guide pipe P1, the outer end of the culture solution outlet 121 is connected with a liquid inlet 122 of the two-way connector 12 at the bottle cap of the microorganism culture bottle 1 through a liquid guide pipe P11, a feeding pump M1 is arranged on the liquid guide pipe P11, and a peristaltic pump is adopted as the feeding pump M1.
In specific implementation, the microorganism culture bottle 1 adopts a four-way interface reagent feeding bottle. The size and the material of the four-way interface reagent feeding bottle are the same as those of the two-way interface reagent feeding bottle. The four-way interface reagent feeding bottle is characterized in that on the basis of the two-way interface reagent feeding bottle, a bottle bottom threaded cap interface 14 and a bottle neck threaded cap interface 15 are respectively arranged at the bottle body. The threaded cap connector 14 at the bottom of the bottle is an air inlet of an air channel, and the threaded cap connector 15 at the bottleneck is an air outlet of the air channel. One of the two-way connectors 12 at the bottle cap is a culture solution inlet 122, the outer end of the culture solution inlet 122 is connected with the liquid guide pipe P11, and a hook is arranged at the inner end of the culture solution inlet 122 and used for hanging 1-3 carriers C. The two-way connector 12 at the bottle cap is a culture solution outlet 123, the inner end of the culture solution outlet 123 is connected with a liquid guide pipe P12 and extends into the bottom of the bottle body of the microorganism culture bottle 1, the outer end of the culture solution outlet 123 is connected with a liquid guide pipe P13, the downstream of the liquid guide pipe P13 is connected with a liquid inlet port 123 of another microorganism culture bottle 1 or a liquid inlet port 124 of a waste liquid collecting bottle 3, the liquid guide pipe P13 is provided with a feeding pump M1, and the feeding pump M1 is a peristaltic pump.
Further, the feed pump M1 precisely drives the culture solution L in the solution supply bottle 2 to be continuously fed to the carrier C through the culture solution inlet 122 of the microorganism culture bottle 1, for example, continuously feeding the culture solution L to the carrier C, periodically feeding the culture solution L to the carrier C, or the like. The liquid supply bottle 2 and the microorganism culture bottle 1 can be connected with liquid guide pipes P10-P19 through liquid guide pipes P10-P19, and the liquid guide pipes can be silica gel pipes. The liquid guide pipes P10-P19 can feed the culture solution L to the microorganism culture flask 1 through dropper-shaped feed ports.
In specific implementation, the liquid supply bottle 2 can be connected to the first gas supply mechanism 4 (i.e. an inert gas bag, such as a nitrogen bag) through a gas duct P20, one end of the gas duct P20 is connected to the inert gas bag, the other end of the gas duct P20 is connected to the inert gas inlet 120 of the liquid supply bottle 2, the outer end of the inert gas inlet 120 is connected to the first gas supply mechanism 4 through a gas duct P2, and the inner end of the inert gas inlet 120 is connected to the gas duct P20 to feed the bottom of the culture solution L. Further, a gas filter F may be disposed on the gas guiding pipe P20 to filter out impurities in the inert gas. Further, a closable valve V1 may be provided in the gas conduit P20.
In a specific embodiment, before the culture solution L in the solution supply bottle 2 is pumped into the microorganism culture bottle 1, the inert gas (e.g., nitrogen gas) in the first gas supply mechanism 4 is used to flush the culture solution L, so as to remove the residual oxygen in the culture solution L and further reduce the oxygen content in the whole system.
Specifically, a waste liquid collecting bottle 3 is provided downstream of the microorganism culture bottle 1, and the waste liquid collecting bottle 3 is used for collecting waste liquid discharged via the carrier C.
In the specific implementation, the culture solution L is fed to the carrier C and expanded on the surface and inside of the carrier C, after the culture solution L is consumed by the microorganisms on the carrier C, the unconsumed culture solution L forms a waste solution together with metabolic waste (such as hydrogen sulfide) discharged by the microorganisms, the waste solution is drained from the carrier C, and the waste solution is collected by the waste solution collecting bottle 3.
In specific implementation, the waste liquid collecting bottle 3 adopts a four-way interface reagent feeding bottle. The four-way interface reagent feeding bottle is designed to be the same as the four-way interface reagent feeding bottle of the microorganism collecting unit 1.
In specific implementation, the liquid supply bottle 2 and the microorganism culture bottle 1, the microorganism culture bottle 1 and the waste liquid collecting bottle 3 and the plurality of microorganism culture bottles 1 can be connected in series in a modularized manner, liquid path connection is carried out through the liquid guide pipes P11-P17, and gas path connection is carried out through the gas guide pipes P23-P25. The catheters P11-P17 and the air ducts P23-P25 can be made of silica gel material.
In the embodiment of the present invention, the carrier C is not immersed in the culture solution L, but exposed to a gaseous medium, and when the culture solution L flows through the carrier C, the culture solution L spreads on the surface and inside of the carrier C, and under the action of gravity, the unconsumed portion of the culture solution L is discharged together with metabolic waste (such as hydrogen sulfide, etc.) discharged from the microorganisms in the carrier C, thereby preventing the accumulation of the metabolic waste and inhibiting the growth of the microorganisms. In addition, the culture solution L can continuously clean the carrier C and also can continuously remove the rapidly growing thalli, thereby maintaining the diversity of the hard-to-culture bacterial population in the enriched flora.
FIG. 2 is a schematic view showing a detailed construction of a microorganism reaction apparatus of the tandem type having a feed gas and off-gas recovery mechanism according to another embodiment of the present invention.
In a specific implementation, as shown in fig. 2, the microbial reactor may further include a first gas supply mechanism 4, a second gas supply mechanism 5, and an exhaust gas recovery mechanism 6.
Specifically, the first gas supply mechanism 4 is connected to the liquid supply bottle 2 through a gas-guide tube P20, and the first gas supply mechanism 4 is used for flushing oxygen dissolved in the culture solution L.
In a specific implementation, the first gas supply means 4 may include an inert gas supply bag or the like. The inert gas may include nitrogen, etc.
In a specific implementation, the air tube P20 may be provided with an air pump M2, and the air pump M2 may pump the inert gas into the liquid supply bottle 2. The air duct P20 can be directly connected to the outer end of the air inlet interface 120 at the bottle cap of the liquid supply bottle 2. The air pump M2 can be a peristaltic pump, and the peristaltic pump can precisely control the amount of inert gas for flushing the culture solution L.
In a specific implementation, an air filter may be disposed on the air duct P20 between the first air supply mechanism 4 and the liquid supply bottle 2. The gas filter may filter out impurities or contaminants in the gaseous medium.
In a specific embodiment, a closable valve V1 may be provided on the air duct P20 between the first air supply mechanism 4 and the liquid supply bottle 2. The valve V1 can be closed to control the opening and closing of the air channel.
Specifically, the second gas supply mechanism 5 is connected to the microorganism culture flask 1 through a gas duct P22, and the second gas supply mechanism 5 is used for supplying a gas medium to the carrier C.
In a specific implementation, the second air supply means 5 may comprise an air supply bag or the like. The gas medium may include methane, hydrogen, carbon dioxide, etc., and may also include a mixture of methane, hydrogen, carbon dioxide, etc.
In specific implementation, an air pump M2 may be disposed on the air duct P22, and the air pump M2 may pump the gas medium into the microorganism culture bottle 1. The air duct P22 can be directly connected with the screw cap air inlet interface 14 at the bottom of the microorganism culture bottle 1 to convey the gas medium into the microorganism culture bottle 1. The air pump M2 may be a peristaltic pump, which can precisely control the amount of air supplied to the carrier C with the gaseous medium.
In a specific implementation, a gas filter may be disposed on the gas-guiding tube P22 between the second gas-supplying mechanism 5 and the microorganism culture bottle 1. The gas filter may filter out impurities and contaminants in the gaseous medium.
In a specific implementation, a closable valve may be arranged on the air duct P22 between the second air supply mechanism 5 and the microorganism culture bottle 1. The valve can be closed to control the opening and closing of the gas path.
Specifically, the waste gas recovery mechanism 6 is connected to the waste liquid collecting bottle 3 through a gas duct P26, and the waste gas recovery mechanism 6 is used for collecting waste gas discharged through the carrier C.
In specific implementation, after the gas medium enters the microorganism culture bottle 1, the gas medium diffuses upwards and diffuses on the surface and inside the carrier C, and gases such as hydrogen sulfide and carbon dioxide discharged by microorganisms are released from the carrier C and discharged from the screw cap interface 15 at the bottleneck.
Fig. 3 schematically shows a detailed structural diagram of a carrier C according to a further embodiment of the present invention.
As shown in fig. 3, the waste liquid collecting bottle 3 can discharge excessive waste liquid through the port 129 at the bottle cap, and the discharged waste liquid can be used for detecting physical and chemical parameters and detecting the growth state of microorganisms.
Specifically, the inner end of the port 129 is connected with a catheter and extends into the bottom of waste liquid in the waste liquid collecting bottle 3, the outer end of the port 129 is connected with a dropper type needle through a catheter P19, the catheter P19 can be provided with a feeding pump M1, the feeding pump M1 can adopt a peristaltic pump to accurately control the discharge amount of the waste liquid, and the catheter P19 can be provided with a closable valve V1 to control the opening and closing of a liquid changing path.
As shown in FIG. 3, one to three gel micro-ball bags can be arranged in the microorganism culture bottle 1 in a hanging manner through hooks. The gel micro-ball bag consists of a non-woven fabric bag B and a plurality of gel-containing micro-balls D, and the gel micro-balls D can provide an enlarged surface area for the attachment of microorganisms, so that K-type microorganisms which are difficult to culture and have slow growth rate can be effectively enriched.
Further, as shown in fig. 3, a rigid material support net S may be wrapped outside the gel microsphere bag. The rigid material support net S may include a rigid plastic net or the like. Furthermore, the hooks are arranged on the upper and lower sides of the rigid material support net S, so that the rigid material support net S can be used as a suspension framework of the gel micro-ball bag, the gel micro-ball bag is prevented from being crushed, and the durability and the stability of the microbial bioreactor are improved.
In specific implementation, the lowest rigid material supporting net S can be hung with a plumb W through a hook to serve as a counterweight, so that all carriers C are positioned on a vertical line, and the culture solution L can be fed to each carrier C.
In a specific implementation, as shown in fig. 3, an air pump M2 may be provided in the air duct P23-P25 between the waste liquid recovery unit 3 and each microorganism culture bottle 1, and the air pump M2 may be a peristaltic pump to precisely control the ventilation amount.
Fig. 4 schematically shows a detailed structure of a bottle cap portion according to still another embodiment of the present invention.
In a specific implementation, as shown in fig. 4, the gas path gas-guide pipes P23-P25 between the waste liquid recovery unit 3 and each microorganism culture bottle 1 may be provided with a check valve V2, and the check valve V2 prevents waste liquid accumulated in the waste liquid recovery unit 3 and/or the microorganism culture bottle 1 from flowing into the gas path.
Specifically, the liquid supply bottle 2, the waste liquid collecting bottle 3 and each microorganism culture bottle 1 are provided with PVC threaded caps 11 of the same specification, the top ends of the threaded caps 11 are provided with round holes, stainless steel two-way connectors 12 and silica gel gaskets 13 of the same specification are stacked in sequence from top to bottom, and the threaded caps 11 can be opened in a rotating mode to balance air pressure or carriers C can be taken out or added through the threaded caps 11.
It is to be understood that the terminology used in the embodiments of the utility model herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the utility model. As used in the examples of the present invention and the appended claims, the singular forms "a", "an", and "the" are intended to include the plural forms as well, and "a" and "an" generally include at least two, but do not exclude at least one, unless the context clearly dictates otherwise.
It should be understood that the term "and/or" as used herein is merely one type of association that describes an associated object, meaning that three relationships may exist, e.g., a and/or B may mean: a exists alone, A and B exist simultaneously, and B exists alone. In addition, the character "/" herein generally indicates that the former and latter related objects are in an "or" relationship.
It should be understood that although the terms first, second, third, etc. may be used in embodiments of the present invention to describe certain elements, these elements should not be limited by these terms. These terms are only used to distinguish one component from another. For example, a first element may also be referred to as a second element, and similarly, a second element may also be referred to as a first element, without departing from the scope of embodiments of the present invention.
The words "if", as used herein, may be interpreted as "at … …" or "at … …" or "in response to a determination" or "in response to a monitoring", depending on the context. Similarly, the phrase "if it is determined" or "if it is monitored (a stated condition or event)" may be interpreted as "when determining" or "in response to determining" or "when monitoring (a stated condition or event)" or "in response to monitoring (a stated condition or event)", depending on the context.
In the embodiments of the present application, "substantially equal to", "substantially perpendicular", "substantially symmetrical", and the like mean that the macroscopic size or relative positional relationship between the two features referred to is very close to the stated relationship. However, it is clear to those skilled in the art that the positional relationship of the object is difficult to be exactly constrained at small scale or even at microscopic angles due to the existence of objective factors such as errors, tolerances, etc. Therefore, even if a slight dot error exists in the size and positional relationship between the two, the technical effect of the present application is not greatly affected.
It is also noted that the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a good or system that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such good or system. Without further limitation, an element defined by the phrase "comprising an … …" does not exclude the presence of additional like elements in the article of commerce or system in which the element is comprised.
In the various embodiments described above, while, for purposes of simplicity of explanation, the methodologies are shown and described as a series of acts, it is to be understood and appreciated by those of ordinary skill in the art that the methodologies are not limited by the order of acts, as some acts may, in accordance with one or more embodiments, occur in different orders and/or concurrently with other acts from that shown and described herein or not shown and described herein, as would be understood by one of ordinary skill in the art.
Those of skill in the art would understand that information, signals, and data may be represented using any of a variety of different technologies and techniques. For example, data, instructions, commands, information, signals, bits (bits), symbols, and chips that may be referenced throughout the above description may be represented by voltages, currents, electromagnetic waves, magnetic fields or particles, optical fields or particles, or any combination thereof.
Those of skill would further appreciate that the various illustrative logical blocks, modules, units, circuits, and algorithm steps described in connection with the embodiments disclosed herein may be implemented as electronic hardware, computer software, or combinations of both. To clearly illustrate this interchangeability of hardware and software, various illustrative components, blocks, modules, units, circuits, and steps have been described above generally in terms of their functionality. Whether such functionality is implemented as hardware or software depends upon the particular application and design constraints imposed on the overall system. Skilled artisans may implement the described functionality in varying ways for each particular application, but such implementation decisions should not be interpreted as causing a departure from the scope of the present invention.
Finally, it should be noted that those skilled in the art will appreciate that embodiments of the present invention set forth numerous technical details for the purpose of providing a better understanding of the present application. However, the technical solutions claimed in the claims of the present application can be basically implemented without these technical details and various changes and modifications based on the above-described embodiments. Accordingly, in actual practice, various changes in form and detail may be made to the above-described embodiments without departing from the spirit and scope of the utility model.
Claims (10)
1. A tandem type microorganism reaction apparatus, comprising a plurality of microorganism culture bottles, a liquid supply bottle and a waste liquid collecting bottle, wherein:
a plurality of microorganism culture bottles are sequentially connected in series, and a carrier suitable for microorganism growth is arranged in each microorganism culture bottle;
the liquid supply bottle is used for continuously supplying culture liquid to the carriers in each microorganism culture bottle and is connected with the upstream of the plurality of microorganism culture bottles;
the waste liquid collecting bottle is used for collecting waste liquid discharged after the microorganism in each microorganism culture bottle grows and is connected with the downstream of the plurality of microorganism culture bottles.
2. The tandem microbial reaction apparatus of claim 1, further comprising: first air feed mechanism, second air feed mechanism and waste gas recovery mechanism, wherein:
the first gas supply mechanism is communicated with the liquid supply bottle through a gas guide tube and is used for conveying inert gas to a position below the liquid level of the culture solution in the liquid supply bottle so as to remove dissolved oxygen in the culture solution;
the second gas supply mechanism is communicated with a bottom gas inlet interface of the first microorganism culture mechanism in a group through a gas guide tube and is used for supplying a gas medium to the carrier;
the waste gas recovery mechanism is communicated with the neck air outlet interface of the waste liquid collecting mechanism through an air duct and is used for collecting waste gas discharged through the carrier.
3. The tandem microbial reaction apparatus of claim 1 wherein the carrier comprises at least one gel microsphere pocket suspended within the interior of the microbial culture flask.
4. The tandem type microorganism reaction apparatus according to claim 3, wherein the gel micro-bag comprises a non-woven bag and a plurality of gel micro-balls, and the plurality of gel micro-balls are filled in the non-woven bag.
5. The tandem type microorganism reaction apparatus according to claim 4, wherein the gel microspheres are agarose microspheres or calcium alginate microspheres.
6. The in-line microbial reaction apparatus of claim 3, wherein a support net is further wrapped outside the gel micro-bag and suspended inside the microbial culture flask by hooks of the support net.
7. The tandem type microorganism reaction apparatus according to claim 1, wherein the liquid supply bottle is a two-way bottle, the two-way bottle comprises a two-way bottle body and a bottle cap portion, and the bottle cap portion comprises a threaded bottle cap with an opening at the top, a two-way port and a silica gel sealing ring.
8. The tandem microbial reaction apparatus of claim 1, wherein the microbial cultivation bottle and the waste liquid collecting bottle are a four-way bottle, the four-way bottle comprises a four-way bottle body and a bottle cap portion, and wherein:
the bottle cap part comprises a threaded bottle cap with an opening at the top, a two-way interface and a silica gel sealing ring;
the bottom and the neck of the four-way bottle body are respectively provided with a threaded cap interface for connecting the air duct.
9. The tandem type microorganism reaction apparatus as claimed in claim 1, wherein the microorganism culture bottles or the liquid supply bottles and the microorganism culture bottles are communicated with each other through a liquid conduit, an upstream port of the liquid conduit is communicated with a liquid outlet port at the bottle cap of the liquid supply bottle or the microorganism culture bottle, a downstream port of the liquid conduit is communicated with a liquid inlet port at the bottle cap of the microorganism culture bottle or the waste liquid collection bottle, and the inner end of each liquid outlet port is connected with a liquid conduit extending into the bottom of the liquid supply bottle, the microorganism culture bottle or the waste liquid collection bottle.
10. The in-line microbial reaction apparatus according to claim 1, wherein the microbial culture bottles or the waste liquid collecting bottle and the microbial culture bottle are communicated through an air duct, an upstream interface of the air duct is communicated with a screw cap interface at a bottle neck of the microbial culture bottle, and a downstream interface of the air duct is communicated with a screw cap interface at a bottle bottom of the connecting microbial culture bottle or a downstream interface of the air duct is communicated with a screw cap interface at a bottle bottom of the waste liquid collecting bottle.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202220006958.1U CN216808800U (en) | 2022-01-04 | 2022-01-04 | Tandem type microorganism reaction equipment |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202220006958.1U CN216808800U (en) | 2022-01-04 | 2022-01-04 | Tandem type microorganism reaction equipment |
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| CN216808800U true CN216808800U (en) | 2022-06-24 |
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| CN202220006958.1U Active CN216808800U (en) | 2022-01-04 | 2022-01-04 | Tandem type microorganism reaction equipment |
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Effective date of registration: 20231130 Address after: 100101 No. 1, West Beichen Road, Beijing, Chaoyang District, 3 Patentee after: INSTITUTE OF MICROBIOLOGY, CHINESE ACADEMY OF SCIENCES Patentee after: CHINA OCEAN MINERAL RESOURCES R & D ASSOCIATION (CHINA'S OCEAN AFFAIRS ADMINISTRATION) Address before: 100101 No. 1, West Beichen Road, Beijing, Chaoyang District, 3 Patentee before: INSTITUTE OF MICROBIOLOGY, CHINESE ACADEMY OF SCIENCES |