CN101935611B - Biomembrane culture and flow chamber in artificial mouth simulation device - Google Patents
Biomembrane culture and flow chamber in artificial mouth simulation device Download PDFInfo
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/12—Well or multiwell plates
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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- C12M23/26—Constructional details, e.g. recesses, hinges flexible
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- C12M23/00—Constructional details, e.g. recesses, hinges
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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Abstract
The invention discloses a biomembrane culture and flow chamber in an artificial mouth simulation device, which comprises a flow chamber base and an upper cover of the flow chamber, wherein, a groove is arranged on the upper surface of the flow chamber base, the size of the groove is matched with the upper cover of the flow chamber, and the upper cover of the flow chamber can be exactly embedded into the groove; the middle part of the groove is equipped with a liquid flow channel, and the liquid flow channel is internally equipped with at least one carrying piece which is the same as or similar to a tooth material; the upper cover of the flow chamber is equipped with a liquid delivery and drainage pipe and a liquid discharge pipe; and the flow chamber base and the upper cover of the flow chamber are detachably fixed through a fixing device. The biomembrane culture and flow chamber of the invention has the following beneficial effects of simple structure, low manufacture cost, simple operation, good pollution control and short service experimental period.
Description
Technical field
The present invention relates to a kind of simulator of oral cavity, the microbial film especially related in a kind of simulating device for artificial oral cavity cultivates flow chamber.
Background technology
For a long time, people carry out bacteriological research in the mode of microorganism pure culture usually, this research greatly simplify the impact of external environment, people are helped to be familiar with the habit of a lot of individual microorganism, achieve much important achievement in research, for tremendous contribution has been made in medical science and microbiological development.But the most of bacterium of occurring in nature is not with the unbound state existence under pure culture, but exist with biomembranous form.Microbial film is that a large amount of germs collect is wrapped in a kind of special cells group structure of being formed in the exocellular polysaccharide of himself secretion on certain dielectric surface, and it can be made up of single bacterium, but under more susceptible condition be various bacteria live in concentrated communities body.With the pre biooxidation ratio of unbound state; biomembranous Clinicopathological significance is even more important; because it has the protectiveness exocellular polysaccharide of one deck densification; therefore there is the ability that stronger resistance and stronger opposing host immune kill and wound; and in microbial film, between different bacterium, also there is the information transport system of a set of complexity; some drug resistant genes can shift between different bacterial classifications, thus increase the difficulty of traditional antibiotic treatment.
Plaque sticks to a kind of microbial film on dental surface, gingival edge, gum inside or other soft tissues of oral cavity.It is made up of a large amount of oral cavity bacterium, extracellular matrix, a small amount of white corpuscle, Exfoliative cells and swill; Its adhere firmly, is difficult to remove.Plaque is considered to the main pathogenic of most of oral disease, comprises carious tooth, gingivitis, periodontitis etc., therefore the research of plaque is become to a focus of stomatology research.In research method, the method for " original position " can reflect truth, but this method actually operating is got up, difficulty is comparatively large, and there is very large uncertainty; So in the research method of oral biological film, what generally adopt is the method for external model.External model can simplify the internal milieu factor of many complexity on the one hand, can realize the autotelic control to biofilm development condition on the other hand.In vitro in model, all include microbial film and cultivate flow chamber or similar device.Such as: Chinese patent 03135320.7, name is called " method that oral cavity bio-film model apparatus and oral biological film thereof are formed " open a kind of biomembranous " constant flow culture ", but should " constant flow culture " be irregular profile, be difficult to accomplish stdn, the clearance rate being difficult in test control each constant flow culture reaches consistent, makes each constant flow culture be difficult to accomplish the parallel comparable of real meaning.Chinese invention patent 200610020608.6 discloses a kind of oral biological film dynamic model device and forms the method for oral biological film, disclose in that patent " constant flow culture " of cultivating for microbial film, the constant flow culture temperature-controllable of this biofilm development, the stability of energy warranty test; But it still has following defect: 1) this constant flow culture complex structure; 2) this constant flow culture cost is produced high; 3) complex operation of this constant flow culture; 4) the Environmental capacity difficulty of this constant flow culture is large; 5) use the test period of the external model of this constant flow culture long.
In sum, all there is certain shortcoming in the flow chamber that existing oral biological film external model uses, and can not meet the needs of research completely.
Summary of the invention
The microbial film that the technical problem to be solved in the present invention is to provide in a kind of simulating device for artificial oral cavity cultivates flow chamber.This flow chamber structure is simple, cost of manufacture is low, simple to operate, Environmental capacity good and use is short for experimental period.
For solving the problems of the technologies described above, the present invention adopts following technical scheme: it comprises flow chamber base and flow chamber upper cover; Described flow chamber base upper surface is provided with groove, and size and the flow chamber upper cover of this groove match, and what described flow chamber upper cover just in time can mate is embedded in groove, plays fitted seal effect each other; The middle part of described groove is provided with liquid-flow groove, is provided with the slide glass that at least one is identical or close with tooth material in this flowing groove; Described flow chamber covers and is provided with the defeated comb of liquid and liquid discharge tube, when carrying out sample test, the defeated comb of described liquid is responsible for sample solution input and expel liquid flowing groove, in general culturing process, nutrient solution is inputted by the defeated comb of liquid, is discharged by liquid discharge tube; Described flow chamber base and flow chamber upper cover are fixed by stationary installation is dismountable.
Further, remittance liquid groove is provided with in described liquid-flow groove; This remittance liquid groove is arranged on position immediately below the defeated comb of described liquid; Because remaining sample solution after the test of medicine antibiont film activity can collect by remittance liquid groove, the nutrient solution after also culturing process can being completed collects; Therefore, remaining sample solution or nutrient solution are all discharged by the defeated comb of liquid; This structure contributes to the remaining sample solution of liquid-flow groove or nutrient solution as far as possible all to discharge, and does not stay residual, and the effect of unlikely impact experiment.
Further, the lower end of the defeated comb of described liquid is provided with flexible pipe, and the bottom of this flexible pipe extends to the bottom of remittance liquid groove; Because remaining sample solution after the test of medicine antibiont film activity can collect by remittance liquid groove, the nutrient solution after also culturing process can being completed collects; Therefore, remaining sample solution or nutrient solution all can be passed through flexible pipe, are then discharged by the defeated comb of liquid; This structure contributes to the remaining sample solution of liquid-flow groove or nutrient solution as far as possible all to discharge, and does not stay residual, and the effect of unlikely impact experiment.
Further, the upper and lower ends termination of the defeated comb of described liquid is provided with arc convex, to facilitate liquid-transport pipe-line to be socketed on the upper end of the defeated comb of liquid, also facilitates flexible pipe to be socketed on the termination, lower end of the defeated comb of liquid.
Further, the structure that the defeated comb of described liquid and liquid discharge tube are formed in one with flow chamber upper cover respectively, in order to avoid the defeated comb of liquid and there is gap between liquid discharge tube and flow chamber upper cover, thus affects the lasting use of device.
Further, in described liquid-flow groove, middle part is provided with retaining plate, and retaining plate sidewall and corresponding liquid-flow groove inner side-wall are provided with the position limiting structure for placing slide glass.The setting of position limiting structure, makes slide glass can be fixed on a locus in liquid-flow groove, unlikelyly slides everywhere, thus the effect of impact experiment.
Further, described retaining plate sidewall is provided with arc-shaped recess line, and described liquid-flow groove inner side-wall is provided with the arc-shaped recess line corresponding to arc-shaped recess line on retaining plate sidewall, and the depression line of two correspondences forms the structure of spacing slide glass.
Further, the outer edge surface of described groove is provided with an annular sulculus, is provided with elastic seal ring in this annular sulculus; Preferably, elastic seal ring is quality of rubber materials.The setting of elastic seal ring, can prevent the nutrient solution in liquid-flow groove from oozing out outside groove, pollute.
Further, described flow chamber base and flow chamber upper cover are fixed by bolt is dismountable; In liquid culture process, can sampling analysis at any time.
Further, the described slide glass identical or close with tooth material is hydroxyapatite flag or germanium wafer.
The present invention has following beneficial effect: structure is simple, cost of manufacture is low, simple to operate, Environmental capacity good and use is short for experimental period.
Accompanying drawing explanation
Fig. 1 is the embodiment of the present invention 1 flow chamber base vertical view;
Fig. 2 is the A-A face sectional view of Fig. 1;
Fig. 3 is the embodiment of the present invention 1 flow chamber upper cover top view;
Fig. 4 is the B-B face sectional view of Fig. 3;
Fig. 5 is decomposition texture schematic diagram of the present invention;
Fig. 6 is embodiment 2 flow chamber base vertical view.
Embodiment
Further the present invention is described in detail below by preferred embodiment.
Embodiment 1
Shown in Fig. 1-5, the present invention includes flow chamber base 1 and flow chamber upper cover 2; Described flow chamber base 1 upper surface is provided with groove 11, and size and the flow chamber upper cover 2 of this groove 11 match, and what described flow chamber upper cover 2 just in time can mate is embedded in groove 11, plays fitted seal effect each other;
The middle part of described groove 11 is provided with liquid-flow groove 111;
The outer edge surface of described groove 11 is provided with annular sulculus 112, is provided with rubber seal 1121 in this annular sulculus 112; The setting of rubber seal 1121, can prevent the nutrient solution in liquid-flow groove 111 from oozing out outside groove, pollute;
Shown in Fig. 3,4,5, described flow chamber upper cover 2 is provided with the defeated comb 21 of liquid and liquid discharge tube 23, when carrying out sample test, the defeated comb 21 of described liquid is responsible for sample solution input and expel liquid flowing groove 111, in general culturing process, nutrient solution is inputted by the defeated comb 21 of liquid, is discharged by liquid discharge tube 23; The structure that the defeated comb 21 of described liquid is formed in one with flow chamber upper cover 2, in order to avoid there is gap between the defeated comb 21 of liquid and flow chamber upper cover 2, thus affects the lasting use of device; The structure that described liquid discharge tube 23 is also formed in one with flow chamber upper cover 2, in order to avoid there is gap between liquid discharge tube 23 and flow chamber upper cover 2, thus affects the lasting use of device; Be provided with arc convex 211,212 outside described liquid defeated comb about 21 two ends, the arc convex 211 outside upper end can conveniently carry the pipeline of nutrient solution to overlap closely on it, also facilitates the outer sheathed flexible pipe 213 of the arc convex 212 of lower end; What show in Fig. 4 is the state that flexible pipe 213 is enclosed within before arc convex 212;
Shown in Fig. 1,2,5, in described liquid-flow groove 111, be provided with 4 pieces of hydroxyapatite flag slide glasses 1111, remittance liquid groove 1112 and retaining plate 1113; Described remittance liquid groove 1112 is arranged on position immediately below the defeated comb 21 of described liquid; Described flexible pipe 213 just in time extends to the bottom of this remittance liquid groove 1112; Because remaining sample solution after the test of medicine antibiont film activity can collect by remittance liquid groove 1112, the nutrient solution after also culturing process can being completed collects; Therefore, remaining sample solution or nutrient solution all can be passed through flexible pipe 213, are then discharged by the defeated comb 21 of liquid; This structure contributes to the remaining sample solution of liquid-flow groove or nutrient solution as far as possible all to discharge, and does not stay residual, and the effect of unlikely impact experiment;
Shown in Fig. 1,2,5, in described liquid-flow groove 111, middle part is provided with retaining plate 1113, described retaining plate 1113 sidewall is provided with arc-shaped recess line 1114, described liquid-flow groove 111 inner side-wall is provided with the structure of the spacing hydroxyapatite flag slide glass 1111 of depression line 1114,1115 formation of corresponding arc-shaped recess line 1115, two correspondence; The setting of position limiting structure, makes hydroxyapatite slide glass 1111 can be fixed on a locus in liquid-flow groove 111, unlikelyly slides everywhere, thus the effect of impact experiment;
Shown in Fig. 1,2,5, described flow chamber base 1 is provided with two threaded holes 12, described flow chamber upper cover 2 is provided with two threaded holes 22 matched with threaded hole 12 position on flow chamber base 1 and size, and described flow chamber base 1 and flow chamber upper cover 2 are fixed together through threaded hole 12 and threaded hole 22 by bolt 13; Therefore, described flow chamber base 1 and flow chamber upper cover 2 are dismountable fixing, therefore in liquid culture process, and can sampling analysis at any time.
Embodiment 2
Shown in Figure 6, repeat embodiment 1, its difference is only, is only provided with 2 pieces of hydroxyapatite flag slide glasses 1111 in described liquid-flow groove 111.
Obviously, the above embodiment of the present invention is only for example of the present invention is clearly described, and is not the restriction to embodiments of the present invention.For those of ordinary skill in the field, can also make other changes in different forms on the basis of the above description.Here cannot give exhaustive to all embodiments.Every belong to technical scheme of the present invention the apparent change of extending out or variation be still in the row of protection scope of the present invention.
Claims (9)
1. the microbial film in simulating device for artificial oral cavity cultivates flow chamber, it is characterized in that: it comprises flow chamber base (1) and flow chamber upper cover (2); Described flow chamber base (1) upper surface is provided with groove (11), size and the flow chamber upper cover (2) of this groove (11) match, and described flow chamber upper cover (2) can be embedded in groove (11) matchingly; The middle part of described groove (11) is provided with liquid-flow groove (111), is provided with the slide glass (1111) that at least one is identical or close with tooth material in this flowing groove (111); Described flow chamber upper cover (2) is provided with the defeated comb of liquid (21) and liquid discharge tube (23); Described flow chamber base (1) and flow chamber upper cover (2) are fixed by stationary installation is dismountable;
Remittance liquid groove (1112) is provided with in described liquid-flow groove (111); This remittance liquid groove (1112) is arranged on position immediately below the defeated comb of described liquid (21).
2. the microbial film in simulating device for artificial oral cavity according to claim 1 cultivates flow chamber, it is characterized in that: the lower end of the defeated comb of described liquid (21) is provided with flexible pipe (213), the bottom of this flexible pipe (213) extends to the bottom of remittance liquid groove (1112).
3. the microbial film in simulating device for artificial oral cavity according to claim 1 cultivates flow chamber, it is characterized in that: the upper and lower end termination of the defeated comb of described liquid (21) is provided with arc convex (211,212).
4. cultivate flow chamber according to the microbial film in described simulating device for artificial oral cavity arbitrary in claims 1 to 3, it is characterized in that: the structure that the defeated comb of described liquid (21) and liquid discharge tube (23) are formed in one with flow chamber upper cover (2) respectively.
5. the microbial film in simulating device for artificial oral cavity according to claim 4 cultivates flow chamber, it is characterized in that: in described liquid-flow groove (111), middle part is provided with retaining plate (1113), retaining plate (1113) sidewall and corresponding liquid-flow groove (111) inner side-wall are provided with the position limiting structure for placing slide glass (1111).
6. the microbial film in simulating device for artificial oral cavity according to claim 5 cultivates flow chamber, it is characterized in that: described retaining plate (1113) sidewall is provided with arc-shaped recess line (1114), described liquid-flow groove (111) inner side-wall is provided with the arc-shaped recess line (1115) corresponding to arc-shaped recess line (1114) on retaining plate (1113) sidewall, and the depression line (1114,1115) of two correspondences forms position limiting structure.
7. the microbial film in simulating device for artificial oral cavity according to claim 1 cultivates flow chamber, it is characterized in that: described groove (11) is provided with an annular sulculus (112) towards the surface of described flow chamber upper cover upward, elastic seal ring (1121) is provided with in this annular sulculus (112), when described flow chamber upper cover (2) embeds described flow chamber base (1), flow chamber described in described elastic seal ring and described flow chamber upper cover (2) fitted seal.
8. the microbial film in simulating device for artificial oral cavity according to claim 1 cultivates flow chamber, it is characterized in that: described flow chamber base (1) and flow chamber upper cover (2) are fixed by bolt (13).
9. the microbial film in simulating device for artificial oral cavity according to claim 1 cultivates flow chamber, it is characterized in that: the described slide glass (1111) identical or close with tooth material is hydroxyapatite flag or germanium wafer.
Priority Applications (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010261075.7A CN101935611B (en) | 2010-08-24 | 2010-08-24 | Biomembrane culture and flow chamber in artificial mouth simulation device |
| EP11819272.3A EP2609189A4 (en) | 2010-08-24 | 2011-08-24 | Methods, devices and uses related to biofilms |
| PCT/CN2011/001409 WO2012024897A1 (en) | 2010-08-24 | 2011-08-24 | Methods, devices and uses related to biofilms |
| US13/773,646 US20130177916A1 (en) | 2010-08-24 | 2013-02-22 | Methods, devices and uses related to biofilms |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201010261075.7A CN101935611B (en) | 2010-08-24 | 2010-08-24 | Biomembrane culture and flow chamber in artificial mouth simulation device |
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| CN101935611A CN101935611A (en) | 2011-01-05 |
| CN101935611B true CN101935611B (en) | 2015-04-22 |
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| CN201010261075.7A Active CN101935611B (en) | 2010-08-24 | 2010-08-24 | Biomembrane culture and flow chamber in artificial mouth simulation device |
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| CN (1) | CN101935611B (en) |
| WO (1) | WO2012024897A1 (en) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101935611B (en) * | 2010-08-24 | 2015-04-22 | 好来化工(中山)有限公司 | Biomembrane culture and flow chamber in artificial mouth simulation device |
| US9562254B2 (en) | 2013-03-19 | 2017-02-07 | Colgate-Palmolive Company | Anti-biofilm screening assays |
| MX2017005792A (en) * | 2014-11-11 | 2017-08-02 | Colgate Palmolive Co | In vitro oral biofilm models of interdental spaces and uses. |
| CN106955754A (en) * | 2016-01-11 | 2017-07-18 | 广州市华代生物科技有限公司 | A kind of biomembrane clamper and its detection method |
| CN105524869B (en) * | 2016-01-19 | 2019-01-25 | 广州立白企业集团有限公司 | A kind of external Dental plaque biofilm model and preparation method thereof |
| RU2661114C1 (en) * | 2017-11-22 | 2018-07-11 | Государственное бюджетное учреждение здравоохранения Московской области "Московский областной научно-исследовательский клинический институт им. М.Ф. Владимирского" (ГБУЗ МО МОНИКИ им. М.Ф. Владимирского) | Method for formation of mixed biofilm of paradontopathogenic anaerobic bacteria in vitro |
| CN108641901B (en) * | 2018-06-07 | 2023-09-15 | 云南中烟工业有限责任公司 | Oral cavity biomembrane external anaerobic model device and application thereof |
| CN117264818B (en) * | 2023-09-15 | 2024-05-17 | 中山大学附属口腔医院 | Oral bacteria biological film culture medium and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1480110A (en) * | 2003-07-01 | 2004-03-10 | 四川大学 | Model of mouth cavity biomembrane and method for forming mouth cavity biomembrane |
| CN1527876A (en) * | 2001-04-27 | 2004-09-08 | Compounds, compositions, and methods for controlling biofilms | |
| CN1858200A (en) * | 2006-03-30 | 2006-11-08 | 四川大学 | Oral biological film dynamic model device and its oral biological film forming method |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| AU5207801A (en) * | 2000-04-17 | 2001-10-30 | Univ Technologies Int | Apparatus and methods for testing effects of materials and surface coatings on the formation of biofilms |
| CN2628532Y (en) * | 2003-07-01 | 2004-07-28 | 四川大学 | Oral cavity bio-film model apparatus |
| US20080166753A1 (en) * | 2004-04-12 | 2008-07-10 | University Technologies International Inc. | Microbial Growth Assay |
| CN101935611B (en) * | 2010-08-24 | 2015-04-22 | 好来化工(中山)有限公司 | Biomembrane culture and flow chamber in artificial mouth simulation device |
| CN101948746B (en) * | 2010-09-09 | 2013-07-10 | 好来化工(中山)有限公司 | Artificial mouth simulating system device |
| CN201834909U (en) * | 2010-08-24 | 2011-05-18 | 好维股份有限公司 | Biomembrane-culturing flow cell in artificial oral model |
| CN201952427U (en) * | 2010-09-09 | 2011-08-31 | 好来化工(中山)有限公司 | Artificial oral-cavity simulating system device |
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2010
- 2010-08-24 CN CN201010261075.7A patent/CN101935611B/en active Active
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1527876A (en) * | 2001-04-27 | 2004-09-08 | Compounds, compositions, and methods for controlling biofilms | |
| CN1480110A (en) * | 2003-07-01 | 2004-03-10 | 四川大学 | Model of mouth cavity biomembrane and method for forming mouth cavity biomembrane |
| CN1858200A (en) * | 2006-03-30 | 2006-11-08 | 四川大学 | Oral biological film dynamic model device and its oral biological film forming method |
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| CN101935611A (en) | 2011-01-05 |
| WO2012024897A1 (en) | 2012-03-01 |
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