CN208829693U - A kind of CAR-T cell culture apparatus - Google Patents

Abstract

The utility model discloses a kind of CAR-T cell culture apparatus, including bottom plate, hose and sampling ware, backboard is installed above the bottom plate, and culture bottle is provided in front of backboard, the culture bottle is fixedly arranged above bottle cap, and the upper center of bottle cap is connected with perfusion tube, the top of the perfusion tube is equipped with feeding solution, the hose is set to the top of bottle cap, and silica gel sealing ring is fixed on the downside of hose, scale is provided on the outside of the culture bottle, and the two sides of culture bottle are equipped with limited block, the bottom of the bottom plate is fixed with non-slip mat, the upper back of the backboard is provided with timer, and fixed plate is installed below timer, the fixed plate is internally provided with placing groove, and through-hole is provided on the outside of placing groove.The CAR-T cell culture apparatus, which is provided with feeding solution, can directly pass through delivered inside culture solution of the perfusion tube to culture bottle, reduce the contaminated possibility of CAR-T cell uncapped and caused in culture bottle.

Description

A kind of CAR-T cell culture apparatus

Technical field

The utility model relates to cell culture apparatus technical field, specially a kind of CAR-T cell culture apparatus.

Background technique

CAR-T cell is a kind of Chimeric antigen receptor T cell, and CAR-T therapy is current more effective malignant tumour One of therapeutic modality, its basic principle are exactly that cancer cell is removed using the immunocyte of patient itself, therefore CAR-T cell Culture apparatus played an important role in medical field.

Existing CAR-T cell culture apparatus is more single, needs manually to inject culture solution use and is inconvenient, and Sampling of uncapping, the ring be easy to causeing in culture bottle are needed when being sampled detection in carrying out CAR-T cell cultivation process Border is unbalance or CAR-T cell is contaminated, so that the culture of CAR-T cell fails, for this purpose, it is proposed that a kind of CAR-T cell Culture apparatus.

Utility model content

The purpose of this utility model is to provide a kind of CAR-T cell culture apparatus, to solve to mention in above-mentioned background technique CAR-T cell culture apparatus out is more single, needs manually to inject culture solution use and is inconvenient, and is carrying out CAR- Need to uncap sampling when being sampled detection in T cell incubation, be easy to cause the environment in culture bottle unbalance or CAR-T cell is contaminated, so that the problem of the culture failure of CAR-T cell.

To achieve the above object, the utility model provides the following technical solutions: a kind of CAR-T cell culture apparatus, including Bottom plate, hose and sampling ware, are equipped with backboard above the bottom plate, and culture bottle is provided in front of backboard, the culture Bottle is fixedly arranged above bottle cap, and the upper center of bottle cap is connected with perfusion tube, and the top of the perfusion tube is equipped with nutrient solution Bottle, the hose is set to the top of bottle cap, and silica gel sealing ring is fixed on the downside of hose, the outside setting of the culture bottle There is a scale, and the two sides of culture bottle are equipped with limited block, the bottom of the bottom plate is fixed with non-slip mat, on the back of the backboard Side is provided with timer, and fixed plate is equipped with below timer, and the fixed plate is internally provided with placing groove, and places Through-hole is provided on the outside of slot, the inside of the through-hole is equipped with negative tube, and negative pressure air pump is fixed with below negative tube, institute It states sampling ware and is installed on the inside of placing groove, the backboard is fixedly arranged above protrusive board, and protrusive board is internally provided with mounting groove.

Preferably, the feeding solution is symmetrical about the vertical centerline of culture bottle, and feeding solution passes through perfusion tube It is connected with the inside of culture bottle.

Preferably, the limited block is equally spaced in the surrounding of culture bottle, and the height of limited block is culture bottle height One third.

Preferably, perpendicular shape structure between the fixed plate and backboard, and the placing groove inside fixed plate is rounded recessed Groove-like structure.

Preferably, the symmetrical centre of the negative pressure air pump is overlapped with the symmetrical centre of sampling ware, and samples ware and placing groove Size match.

Preferably, to be fixedly connected between the protrusive board and backboard, and the mounting groove inside protrusive board mutually blocks with feeding solution It closes.

Compared with prior art, the utility model has the beneficial effects that the CAR-T cell culture apparatus is provided with nutrient solution Bottle can directly pass through delivered inside culture solution of the perfusion tube to culture bottle, reduce to uncap and cause the CAR-T in culture bottle thin The contaminated possibility of born of the same parents, feeding solution can be fastened in the mounting groove inside protrusive board, and CAR-T cell is used for inside culture bottle Culture, culture bottle internal capacity for culture dish is larger, and four culture bottles can both be cultivated simultaneously, can also be with timesharing Section culture is convenient for comparing, and timer can recorde the time that culture bottle CAR-T cell is cultivated, and four timers can be right Corresponding culture bottle carries out time record respectively, and scale can be avoided convenient for the cell-volume inside display culture bottle, limited block The movement of culture bottle, so that culture bottle placement is more stable, the rounded groove-like structure of placing groove takes convenient for placing inside it Sample ware, sampling ware are connected by hose with the inside of culture bottle, silica gel sealing ring with leakproofness, can prevent bottle cap with it is soft The cultivation conditions for not influencing cell closely are connected between pipe, negative pressure air pump can extract the air inside sampling ware by negative tube Keep it internal in negative pressure state, then under the action of sampling pressure difference inside and outside ware, hose is by the CAR-T cell inside culture bottle It is transported in sampling ware, it is convenient that inspection is sampled to the CAR-T cell inside culture bottle, it avoids directly opening culture bottle and cause The imbalance of culture bottle internal balance environment, non-slip mat is capable of increasing frictional force and makes bottom plate not easily shifted.

Detailed description of the invention

Fig. 1 is the utility model positive structure schematic；

Fig. 2 is the utility model back structures schematic diagram；

Fig. 3 is the utility model overlooking structure diagram.

In figure: 1, bottom plate, 2, backboard, 3, culture bottle, 4, bottle cap, 5, perfusion tube, 6, feeding solution 7, hose, 8, silica gel Sealing ring, 9, scale, 10, limited block, 11, non-slip mat, 12, fixed plate, 13, placing groove, 14, timer, 15, through-hole, 16, negative Pressure pipe, 17, negative pressure air pump, 18, sampling ware, 19, protrusive board, 20, mounting groove.

Specific embodiment

The following will be combined with the drawings in the embodiments of the present invention, carries out the technical scheme in the embodiment of the utility model Clearly and completely describe, it is clear that the described embodiments are only a part of the embodiments of the utility model, rather than whole Embodiment.Based on the embodiments of the present invention, those of ordinary skill in the art are without making creative work Every other embodiment obtained, fall within the protection scope of the utility model.

Fig. 1-3 is please referred to, the utility model provides a kind of technical solution: a kind of CAR-T cell culture apparatus, including bottom plate 1, backboard 2, culture bottle 3, bottle cap 4, perfusion tube 5, feeding solution 6, hose 7, silica gel sealing ring 8, scale 9, limited block 10, anti-skidding Pad 11, fixed plate 12, placing groove 13, timer 14, through-hole 15, negative tube 16, negative pressure air pump 17, sampling ware 18,19 and of protrusive board Mounting groove 20, the top of bottom plate 1 is equipped with backboard 2, and the front of backboard 2 is provided with culture bottle 3, and the top of culture bottle 3 is fixed There is bottle cap 4, and the upper center of bottle cap 4 is connected with perfusion tube 5, the top of perfusion tube 5 is equipped with feeding solution 6, feeding solution 6 Vertical centerline about culture bottle 3 is symmetrical, and feeding solution 6 is connected by perfusion tube 5 with the inside of culture bottle 3, battalion Nutrition is convenient in the vertical centerline symmetrical surface that makes feeding solution 6 be located at culture bottle 3 of the nutrient solution bottle 6 about culture bottle 3 Nutriment needed for liquid bottle 6 passes through delivered inside CAR-T cell culture of the perfusion tube 5 to culture bottle 3, hose 7 are set to bottle The top of lid 4, and the downside of hose 7 is fixed with silica gel sealing ring 8, the outside of culture bottle 3 are provided with scale 9, and culture bottle 3 Two sides are equipped with limited block 10, and limited block 10 is equally spaced in the surrounding of culture bottle 3, and the height of limited block 10 is culture bottle 3 The one third of height, the surrounding in culture bottle 3 that is equally spaced of limited block 10 can play the role of limit to culture bottle 3, keep away Exempt from the movement of culture bottle 3, the height of limited block 10 is that the one third of 3 height of culture bottle can either play the role of limit, again Taking for culture bottle 3 is not influenced, the bottom of bottom plate 1 is fixed with non-slip mat 11, and the upper back of backboard 2 is provided with timer 14, And the lower section of timer 14 is equipped with fixed plate 12, perpendicular shape structure between fixed plate 12 and backboard 2, and inside fixed plate 12 The rounded groove-like structure of placing groove 13, between fixed plate 12 and backboard 2 perpendicular shape structure enable sampling ware 18 put down Steady to place, the rounded groove-like structure of placing groove 13 inside fixed plate 12 matches with circular 18 shape of sampling ware, fixed Plate 12 is internally provided with placing groove 13, and the outside of placing groove 13 is provided with through-hole 15, and the inside of through-hole 15 is equipped with negative pressure Pipe 16, and the lower section of negative tube 16 is fixed with negative pressure air pump 17, in the symmetrical centre of negative pressure air pump 17 and the symmetrical of sampling ware 18 The heart is overlapped, and is sampled ware 18 and matched with the size of placing groove 13, the symmetrical centre of negative pressure air pump 17 with sample the symmetrical of ware 18 Center is overlapped so that negative pressure air pump 17 is located at the underface of sampling ware 18 convenient for the sky inside the extraction sampling ware 18 of negative pressure air pump 17 Gas, sampling ware 18, which matches to allow with the size of placing groove 13, samples the inside that ware 18 is placed on placing groove 13, samples ware 18 are installed on the inside of placing groove 13, backboard 2 is fixedly arranged above protrusive board 19, and protrusive board 19 is internally provided with mounting groove 20, To be fixedly connected between protrusive board 19 and backboard 2, and the mounting groove 20 protrusive board 19 inside fastens with feeding solution 6, protrusive board 19 and Fasten its installation more to be fixedly connected between backboard 2, the mounting groove 20 inside protrusive board 19 fasten with feeding solution 6 so that Feeding solution 6 can be upside down in mounting groove 20 and be not easy to fall.

Working principle: collected CAR-T cell is injected into advance by this kind of CAR-T cell culture apparatus first In the culture bottle 3 of sterilization, then closed closure 4, the placement bottom plate 1 of culture bottle 3 are placed between limited block 10, then Configured culture solution is injected in feeding solution 6, then feeding solution 6 is upside down in inside mounting groove 20, is then set defeated Then sampling ware 18 is placed into putting inside fixed plate 12 so that perfusion tube 5 is connected to feeding solution 6 and culture bottle 3 by liquid pipe 5 It sets in slot 13, and sampling ware 18 is connected with negative tube 16, and negative pressure air pump 17 will be installed at the lower end of negative tube 16, Then it is connected to culture bottle 3 and sampling ware 18 using hose 7, and is fixed in perfusion tube 5 and hose 7 and the junction of bottle cap 4 Then silica gel sealing ring 8 can star timer 14 and start to carry out timing, the culture of CAR-T cell is needed in constant temperature and humidity Indoor progress can be understood in display culture bottle 3 during cultivating CAR-T cell by the scale 9 in 3 outside of culture bottle The cell-volume in portion can star negative pressure air pump 17 when needing to carry out cell sampling detection, and negative pressure air pump 17 is led to It crosses negative tube 16 and extracts the air sampled inside ware 18, make to sample 18 internal negative pressure state of ware, then the external pressure in sampling ware 18 It is strong it is poor under the action of, hose 7 by CAR-T cell delivery culture bottle 3 inside to sampling in ware 18, inside culture bottle 3 CAR-T cell is sampled inspection, and it is unbalance to avoid directly opening 3 internal environment of culture bottle caused by culture bottle 3, completes like this The use process of entire CAR-T cell culture apparatus.

While there has been shown and described that the embodiments of the present invention, for the ordinary skill in the art, It is understood that these embodiments can be carried out with a variety of variations in the case where not departing from the principles of the present invention and spirit, repaired Change, replacement and variant, the scope of the utility model is defined by the appended claims and the equivalents thereof.

Claims (6)

1. a kind of CAR-T cell culture apparatus, including bottom plate (1), hose (7) and sampling ware (18), it is characterised in that: the bottom It is equipped with above plate (1) backboard (2), and is provided with culture bottle (3) in front of backboard (2), the top of the culture bottle (3) is solid Surely have bottle cap (4), and the upper center of bottle cap (4) is connected with perfusion tube (5), the top installation of the perfusion tube (5) is nutritious Liquid bottle (6), the hose (7) is set to the top of bottle cap (4), and silica gel sealing ring (8), institute are fixed on the downside of hose (7) It states and is provided on the outside of culture bottle (3) scale (9), and the two sides of culture bottle (3) are equipped with limited block (10), the bottom plate (1) Bottom be fixed with non-slip mat (11), the upper back of the backboard (2) is provided with timer (14), and under timer (14) Side is equipped with fixed plate (12), and the fixed plate (12) is internally provided with placing groove (13), and sets on the outside of placing groove (13) It is equipped with through-hole (15), the inside of the through-hole (15) is equipped with negative tube (16), and is fixed with negative pressure below negative tube (16) Air pump (17), sampling ware (18) are installed on the inside of placing groove (13), and the backboard (2) is fixedly arranged above protrusive board (19), and protrusive board (19) is internally provided with mounting groove (20).

2. a kind of CAR-T cell culture apparatus according to claim 1, it is characterised in that: the feeding solution (6) about The vertical centerline of culture bottle (3) is symmetrical, and feeding solution (6) is connected by perfusion tube (5) with the inside of culture bottle (3) It is logical.

3. a kind of CAR-T cell culture apparatus according to claim 1, it is characterised in that: the limited block (10) is equidistant It is distributed in the surrounding of culture bottle (3), and the height of limited block (10) is the one third of culture bottle (3) height.

4. a kind of CAR-T cell culture apparatus according to claim 1, it is characterised in that: the fixed plate (12) and back Perpendicular shape structure between plate (2), and the rounded groove-like structure of placing groove (13) that fixed plate (12) is internal.

5. a kind of CAR-T cell culture apparatus according to claim 1, it is characterised in that: the negative pressure air pump (17) Symmetrical centre is overlapped with the symmetrical centre of sampling ware (18), and is sampled ware (18) and matched with the size of placing groove (13).

6. a kind of CAR-T cell culture apparatus according to claim 1, it is characterised in that: the protrusive board (19) and backboard (2) to be fixedly connected between, and the mounting groove (20) of protrusive board (19) inside fastens with feeding solution (6).