CN212223015U - Cell culture device of convenient interim sample - Google Patents
Cell culture device of convenient interim sample Download PDFInfo
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- CN212223015U CN212223015U CN202020690805.4U CN202020690805U CN212223015U CN 212223015 U CN212223015 U CN 212223015U CN 202020690805 U CN202020690805 U CN 202020690805U CN 212223015 U CN212223015 U CN 212223015U
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Abstract
The utility model relates to a cell culture's technical field specifically is a cell culture device of convenient interim sample, including cultivateing box and erection column, the erection column runs through and installs in the centre of cultivateing the box. The sealing cover is arranged at the top end of the culture box body, the sealing ring is embedded on the surface of the sealing cover, and the sealing strip is arranged on the part of the mounting column penetrating through the sealing cover, so that the sealing type of the whole culture box body is ensured, and the influence of air entering the culture box body to culture cells in the culture process is avoided; be equipped with division board, rotor plate and sample groove through the inside at the erection column, be equipped with the carousel in the bottom of erection column, and then the position of adjustment closing plate and jack through the rotation of closing plate, and then make the sample groove can take a sample to culture dish surface culture's cell, and the division board is kept apart the sample groove simultaneously, avoids the in-process air that takes a sample to enter into the surface of culture dish and then causes secondary pollution to the cell of cultivateing.
Description
Technical Field
The utility model relates to a cell culture's technical field specifically is a cell culture device of convenient interim sample.
Background
Cellular immunotherapy is a technology rapidly developed in the field of tumor medicine, and is a fourth treatment technology after three traditional treatments of surgery, radiotherapy and chemotherapy. The cell immunotherapy comprises collecting peripheral blood of patient, separating out immunocyte, inducing culture in vitro to expand a large amount, and finally infusing back to patient to kill tumor cells. Therefore, different cells need to be cultured to meet the requirements of cellular immunotherapy.
In the conventional culture method, when the growth state of the cultured cells is monitored from time to time or the expression of the target protein of the cells is measured, it is necessary to take a sample from the culture apparatus at a timing during the culture. In the operation, the closed culture bottle mouth needs to be opened, and the bottle mouth is sterilized and closed after the sampling is finished, so that the operation is relatively complicated, and secondary pollution is easily caused.
SUMMERY OF THE UTILITY MODEL
Technical problem
To sum up, the utility model provides a cell culture device of convenient interim sample to current in solving above-mentioned background art will be confined to cultivate the bottleneck and open carrying out the sample in-process to cell culture device, the bottleneck of disinfecting again and seal after the sample ends, the operation is loaded down with trivial details relatively, and causes secondary pollution's problem easily.
(II) technical scheme
The utility model provides a cell culture device of convenient interim sample, including cultivateing box and erection column, the erection column runs through to be installed in the centre of cultivateing the box:
the culture box body comprises a sealing cover, a sealing ring, a culture dish, a support column and a mounting disc, wherein the sealing cover is movably mounted at the top end of the culture box body, a layer of sealing ring is embedded on the surface of the sealing cover, the culture dish is fixedly mounted in the middle of the interior of the culture box body, the mounting disc is arranged in the middle of the surface of the culture dish, the support column is fixedly connected to the bottom surface of the culture dish, and the other end of the support column is fixedly connected to the bottom end of the interior of the culture box body;
the erection column includes handle, bull stick, division board, closing plate, carousel, sample groove, rotor plate, spliced pole, jack, sealing strip, the handle is installed to the top activity detachable of erection column, the centre fixed mounting of handle has the bull stick, the erection column activity runs through in the middle of the surface of closing cap, the contact department movable mounting of erection column and closing cap has the sealing strip, the bottom fixedly connected with division board of bull stick, the bottom fixed mounting of division board has the spliced pole, the other end fixed connection of spliced pole is in the sample groove, the surface of erection column is equipped with the jack, the bottom of erection column is run through and is cultivateed the box and rotate and connect in the carousel, the fixed surface of carousel is connected with the rotor plate, the other end fixedly connected with closing plate of rotor plate.
Preferably, the bottom end of the sampling groove is movably connected to the surface of the mounting plate.
Preferably, the middle of the mounting column penetrates through the middle of the surface of the culture dish, and a layer of sealing strip is embedded at the contact part of the mounting column and the culture dish.
Preferably, the isolation plate is movably mounted in the middle of the inner part of the mounting column.
Preferably, the mounting column is in a through state, the surface of the mounting column is provided with the insertion hole, and the rest surface of the mounting column is a closed surface.
Preferably, the top surface of erection column is equipped with the screw thread, and the bottom of handle is equipped with the screw hole that corresponds with the screw thread, and the top of erection column is through screw thread activity demountable installation in the screw hole in the bottom of handle.
Preferably, the sealing plate is rotatably mounted to an outer surface of the sampling groove.
Preferably, the surface area of the socket of the mounting post surface is 5-10 square centimeters less than the surface area of the sealing plate.
Preferably, the incubator body and the mounting post are both made of transparent glass with high visibility.
(III), beneficial effect:
1. at first, the utility model discloses a top at the cultivation box is equipped with the closing cap, and surface mosaic at the closing cap has the sealing washer simultaneously, and the part that the erection column runs through the closing cap simultaneously also is equipped with the sealing strip, has guaranteed the canned type of whole cultivation box, avoids having air entering cultivation box influence to the cultivation of cell in the cultivation process.
2. Secondly, the utility model discloses an inside at the erection column is equipped with the division board, rotor plate and sample groove, bottom at the erection column is equipped with the carousel, rotation through the carousel drives the rotor plate and then drives the closing plate and rotate, rotation through the closing plate and then the position of adjustment closing plate and jack, and then make the sample groove can take a sample the cell of culture dish surface culture, the division board keeps apart the sample groove simultaneously, avoid having air to enter into the surface of culture dish and then cause secondary pollution to the cell of cultivateing at the in-process of sample.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings needed to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present invention and therefore should not be considered as limiting the scope, wherein the arrow represents the direction of its movement, and for those skilled in the art, other related drawings can be obtained according to these drawings without inventive efforts.
Fig. 1 is a schematic view of the connection of the whole section of the present invention.
Fig. 2 is a schematic view of the connection between the middle isolation plate and the sampling slot of the present invention.
Fig. 3 is an explosion diagram of the middle mounting post and the sampling slot of the present invention.
FIG. 4 is a schematic view of the connection of the culture dish of the present invention.
In the figure: 1-culture box body, 2-sealing cover, 3-sealing ring, 4-culture dish, 5-handle, 6-mounting column, 7-rotating rod, 8-isolation plate, 9-sealing plate, 10-rotating disc, 11-sampling groove, 12-rotating plate, 13-connecting column, 14-jack, 15-support column, 16-mounting disc and 17-sealing strip.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
Example (b):
referring to fig. 1 to 4, a cell culture apparatus for facilitating temporary sampling includes a culture box 1 and a mounting post 6, the mounting post 6 is installed in the middle of the culture box 1:
the culture box body 1 comprises a sealing cover 2, a sealing ring 3, a culture dish 4, a support column 15 and a mounting disc 16, wherein the sealing cover 2 is movably mounted at the top end of the culture box body 1, the surface of the sealing cover 2 is inlaid with a layer of sealing ring 3, the culture dish 4 is fixedly mounted in the middle of the interior of the culture box body 1, the mounting disc 16 is arranged in the middle of the surface of the culture dish 4, the support column 15 is fixedly connected to the bottom surface of the culture dish 4, and the other end of the support column 15 is fixedly connected to the bottom end of the interior;
Wherein, the bottom end of the sampling groove 11 is movably connected with the surface of the mounting plate 16.
Wherein, the centre of erection column 6 runs through in the middle of the surface of culture dish 4, and erection column 6 is inlayed with culture dish 4 contact department and is had the one deck sealing strip, avoids having the air can enter into the surface of culture dish 4 through the pore of run-through part, causes secondary pollution to the cell on culture dish 4 surface.
Wherein, division board 8 movable mounting is in the middle of the inside of erection column 6, can avoid the air to enter into the surface of culture dish 4 and cause secondary pollution to the cell when sampling groove 11 samples the cell on culture dish 4 surface.
Wherein, the inside of erection column 6 is the through state, and surface mounting has jack 14, and other surfaces are the enclosed surface, can effectively keep apart to the external world.
Wherein, the top surface of erection column 6 is equipped with the screw thread, and the bottom of handle 5 is equipped with the screw hole that corresponds with the screw thread, and the top of erection column 6 is through screw thread activity demountable installation in the screw hole in the bottom of handle 5.
Wherein, the sealing plate 9 is rotatably installed on the outer surface of the sampling groove 11, and the outer end of the sampling groove 11 can be isolated or penetrated through by the rotation of the sealing plate 9.
The surface area of the insertion hole 14 on the surface of the mounting column 6 is 5-10 square centimeters smaller than that of the sealing plate 9, so that the sealing plate 9 can seal the insertion hole 14 on the surface of the mounting column 6 conveniently.
Wherein, the culture box body 1 and the mounting column 6 are made of transparent glass materials with high visibility, and can be convenient for sampling cells on the surface of the culture dish 4.
The working principle is as follows: the culture personnel carry out disinfection treatment on the whole device, lift the sealing cover 2 on the surface of the mounting column 6 to ensure that the sealing cover 2 has enough clearance with the culture box body 1, place the cells to be cultured on the culture dish 4 for culture, and seal the sealing cover 2 at the top end of the culture box body 1 through the sealing ring 3; when the cell is needed to be sampled and detected, the rotary disc 10 at the bottom end of the culture box body 1 is rotated, the rotary disc 10 drives the rotary plate 12 to rotate, the rotary plate 12 drives the sealing plate 9 to rotate, the sealing plate 9 is rotated to the inner side of the mounting column 6, the jack 14 on the surface of the mounting column 6 is exposed, further, the sampling groove 11 is contacted with the cells in the culture dish 4 through the jack 14 to sample the cells, the isolation plate 8 at the upper end of the sampling groove 11 isolates the external air during the sampling process, the air is prevented from flowing into the culture dish 4 during the sampling process, the rotary disk 10 is rotated again after the sampling is finished, make closing plate 9 seal processing with jack 14 through the rotation of carousel 10, rotate handle 5 and erection column 6 after the sealing and dismantle, mention handle 5 after the dismantlement upwards, take out sample groove 11 from erection column 6, and then can be convenient for the staff observes the cell of taking a sample in to sample groove 11.
Claims (9)
1. The utility model provides a cell culture device of convenient interim sample, includes culture box (1) and erection column (6), erection column (6) run through install in the centre of culture box (1), its characterized in that:
the culture box body (1) comprises a sealing cover (2), a sealing ring (3), a culture dish (4), a support column (15) and a mounting disc (16), wherein the sealing cover (2) is movably mounted at the top end of the culture box body (1), a layer of sealing ring (3) is embedded on the surface of the sealing cover (2), the culture dish (4) is fixedly mounted in the middle of the interior of the culture box body (1), the mounting disc (16) is arranged in the middle of the surface of the culture dish (4), the support column (15) is fixedly connected to the bottom surface of the culture dish (4), and the other end of the support column (15) is fixedly connected to the bottom end of the interior of the culture box body (1);
the mounting column (6) comprises a handle (5), a rotating rod (7), a partition plate (8), a sealing plate (9), a turntable (10), a sampling groove (11), a rotating plate (12), a connecting column (13), a jack (14) and a sealing strip (17), the handle (5) is movably and detachably mounted at the top end of the mounting column (6), the rotating rod (7) is fixedly mounted in the middle of the handle (5), the mounting column (6) movably penetrates through the middle of the surface of the sealing cover (2), the sealing strip (17) is movably mounted at the contact part of the mounting column (6) and the sealing cover (2), the partition plate (8) is fixedly connected to the bottom end of the rotating rod (7), the connecting column (13) is fixedly mounted at the bottom end of the partition plate (8), the other end of the connecting column (13) is fixedly connected to the sampling groove (11), the jack (14) is arranged on the surface of the mounting column (6), the bottom end of the mounting column (6) penetrates through the culture box body (1) to be rotatably connected to the rotary table (10), the surface of the rotary table (10) is fixedly connected with a rotating plate (12), and the other end of the rotating plate (12) is fixedly connected with a sealing plate (9).
2. A cell culture apparatus for facilitating temporary sampling according to claim 1, wherein: the bottom end of the sampling groove (11) is movably connected to the surface of the mounting disc (16).
3. A cell culture apparatus for facilitating temporary sampling according to claim 1, wherein: the middle of erection column (6) runs through in the middle of the surface of culture dish (4), and it has one deck sealing strip to inlay in erection column (6) and culture dish (4) contact department.
4. A cell culture apparatus for facilitating temporary sampling according to claim 1, wherein: the isolation plate (8) is movably arranged in the middle of the inner part of the mounting column (6).
5. A cell culture apparatus for facilitating temporary sampling according to claim 1, wherein: the interior of the mounting column (6) is in a through state, the surface of the mounting column is provided with a jack (14), and the rest surfaces are closed surfaces.
6. A cell culture apparatus for facilitating temporary sampling according to claim 1, wherein: the top surface of erection column (6) is equipped with the screw thread, and the bottom of handle (5) is equipped with the screw hole that corresponds with the screw thread, and the top of erection column (6) passes through screw thread activity demountable installation in the screw hole in the bottom of handle (5).
7. A cell culture apparatus for facilitating temporary sampling according to claim 1, wherein: the sealing plate (9) is rotatably arranged on the outer surface of the sampling groove (11).
8. A cell culture apparatus for facilitating temporary sampling according to claim 1, wherein: the surface area of the insertion hole (14) on the surface of the mounting column (6) is 5-10 square centimeters smaller than that of the sealing plate (9).
9. A cell culture apparatus for facilitating temporary sampling according to claim 1, wherein: the culture box body (1) and the mounting column (6) are made of transparent glass materials with high visibility.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020690805.4U CN212223015U (en) | 2020-04-29 | 2020-04-29 | Cell culture device of convenient interim sample |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020690805.4U CN212223015U (en) | 2020-04-29 | 2020-04-29 | Cell culture device of convenient interim sample |
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| Publication Number | Publication Date |
|---|---|
| CN212223015U true CN212223015U (en) | 2020-12-25 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202020690805.4U Active CN212223015U (en) | 2020-04-29 | 2020-04-29 | Cell culture device of convenient interim sample |
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| CN (1) | CN212223015U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112522104A (en) * | 2020-12-29 | 2021-03-19 | 弘康信达(江苏)医学研究有限公司 | Mesenchymal stem cell culture container and culture method |
| CN117946857A (en) * | 2024-03-27 | 2024-04-30 | 山东格林医学科技有限公司 | CAR-NK cell culture device and method |
-
2020
- 2020-04-29 CN CN202020690805.4U patent/CN212223015U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112522104A (en) * | 2020-12-29 | 2021-03-19 | 弘康信达(江苏)医学研究有限公司 | Mesenchymal stem cell culture container and culture method |
| CN117946857A (en) * | 2024-03-27 | 2024-04-30 | 山东格林医学科技有限公司 | CAR-NK cell culture device and method |
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| GR01 | Patent grant | ||
| CP01 | Change in the name or title of a patent holder |
Address after: Floor 1 and 2, building 8, Ganzhou medical equipment Industrial Park, No.1, Wudangshan Road, Shahe Town, Zhanggong District, Ganzhou City, Jiangxi Province Patentee after: Jiangxi Jianlan Biomedical Technology Co.,Ltd. Address before: Floor 1 and 2, building 8, Ganzhou medical equipment Industrial Park, No.1, Wudangshan Road, Shahe Town, Zhanggong District, Ganzhou City, Jiangxi Province Patentee before: Jiangxi baiaotec Biomedical Technology Co.,Ltd. |
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| CP01 | Change in the name or title of a patent holder |