CN113499274A

CN113499274A - Intelligent preparation method of human stem cell factor skin repair liquid

Info

Publication number: CN113499274A
Application number: CN202110757051.9A
Authority: CN
Inventors: 梅建勋; 欧阳建华; 汪凯; 何伟贤; 付引梅; 谭慕华; 梁键全
Original assignee: Guangdong Mercells Cell Biotechnology Co ltd
Current assignee: Guangdong Mercells Cell Biotechnology Co ltd
Priority date: 2021-07-05
Filing date: 2021-07-05
Publication date: 2021-10-15

Abstract

The invention provides an intelligent preparation method of a human stem cell factor skin repair liquid, which is completed by matching an incubator, and comprises the following steps: 1) aseptically collecting umbilical cord blood, and placing the umbilical cord blood into an incubator for culture by adopting a tissue block direct culture method; 2) after the tissue blocks are discarded, replacing a fresh serum-free culture medium and continuously putting the culture medium into the culture box for culture; 3) and after 90% of cells are fused, collecting supernatant, adding pancreatin for digestion, and centrifuging to obtain the cells. The invention can automatically detect the cells in the culture dish, avoids frequent detection of workers, reduces the frequency of opening the first door body, reduces the labor intensity of people, greatly reduces the communication area and time between the box body and the outside, reduces air exchange, thereby facilitating stable control of the temperature and humidity and the gas content in the box body, reducing fluctuation and energy consumption, having higher stability and being convenient for people to use.

Description

Intelligent preparation method of human stem cell factor skin repair liquid

Technical Field

The invention relates to the technical field of preparation of skin repair liquid, in particular to an intelligent preparation method of human stem cell factor skin repair liquid.

Background

Stem cells (stem cells) are a type of pluripotent cells having self-renewal and self-replicating abilities (self-replicating), and stem cells can be classified into embryonic stem cells and adult stem cells, and classified into totipotent stem cells, pluripotent stem cells and unipotent stem cells according to their differentiation potentials, and have the characteristics of multipotential differentiation potential, unlimited division, proliferation, etc., and simply, they are a type of primitive undifferentiated cells having multipotential differentiation potential and self-replicating ability, and are primitive cells forming tissues and organs of mammals. The stem cells have common morphology, are usually circular or oval, have small cell volume and relatively large nuclei, mostly have euchromatin and have high telomerase activity. Stem cells can be divided into embryonic stem cells and adult stem cells;

the aging of the human body and the appearance of wrinkles are essentially the aging and reduction of cells. The aging and reduction of cells is caused by the aging of stem cells. The stem cell is a seed cell for the renewal of various histiocytes and is a production plant of human cells. The aging of the stem cell population seriously weakens the proliferation and differentiation capacity of the stem cell population, the newly born cells are not supplemented enough, the aged cells cannot be replaced in time, and the functions of all systems of the whole body are reduced, so that people can age all the day by day. The skin cannot be timely renewed due to aging of the skin stem cells, and the aged skin cannot be repaired, so that the skin repairing liquid is prepared by people based on the human stem cells;

in the preparation of the existing skin repair liquid, the skin repair liquid is mostly prepared by human stem cell culture and proliferation, and during preparation, cells need to be placed in a culture medium and then placed in an incubator to keep certain conditions such as temperature, humidity, carbon dioxide concentration, oxygen concentration and the like;

but current incubator simple structure, when people take out the cultivation sample, need often observe the in-box cell proliferation condition and the in service behavior of nutrient solution for nutrient solution disappearance or cell proliferation quantity are too big, need change nutrient solution or dilute the cell, people just need open the incubator and take out the change, the incubator is opened, lead to incubator inside air and outside intercommunication, lead to the humiture and the gaseous content unbalance in the incubator, influence the cultivation of cell.

Therefore, there is a need to provide an intelligent preparation method of a human stem cell factor skin repair liquid to solve the above technical problems.

Disclosure of Invention

In order to solve the technical problems, the invention provides an intelligent preparation method of a human stem cell factor skin repair liquid.

The invention provides an intelligent preparation method of a human stem cell factor skin repair liquid, which is completed by matching an incubator, and comprises the following steps:

1) aseptically collecting umbilical cord blood, and placing the umbilical cord blood into an incubator for culture by adopting a tissue block direct culture method;

2) after the tissue blocks are discarded, replacing a fresh serum-free culture medium and continuously putting the culture medium into the culture box for culture;

3) after 90% of cells are fused, collecting supernatant, adding pancreatin for digestion, and centrifuging to obtain cells;

4) inoculating the cells into a culture device for culture, and adding a serum-free culture medium for continuous passage;

5) collecting cell culture solution, centrifuging, and filtering with 0.2 μm pore size filter;

6) screening the supernatant according to the molecular weight index to obtain a mixture consisting of an epidermal growth factor with the molecular weight of 6.201KD, an acidic fibroblast growth factor with the molecular weight of 15.3KD, a basic fibroblast growth factor with the molecular weight of 16-18.5 KD and a vascular endothelial growth factor with the molecular weight of 32-45 KD;

wherein, the incubator in the step 1) comprises a box body, a first clapboard, a grooved pulley reducer, a limiting mechanism, a driving mechanism, a turntable, a sliding groove, a placing block, a placing groove, a culture dish, a slideway, a first sliding block, a first driving block, a second driving block, a door opening, a first door body, a second clapboard, a second door body, a mounting block and an electron microscope, the first clapboard is fixed at the lower end of the inner wall of the box body, the grooved pulley reducer is fixed at the lower surface of the first clapboard, the output end of the grooved pulley reducer passes through the first clapboard and is fixed with the turntable, the sliding grooves are equidistantly arranged on the surface of the turntable, the placing block is connected with the inner wall of the sliding groove in a sliding way, the placing groove is arranged on the inner wall of the placing groove, the slideway is arranged at the bottom of the inner wall of the sliding groove, the first sliding block is fixed at the bottom of the placing block and is arranged in an arc shape, first baffle upper surface one end is fixed with actuating mechanism, actuating mechanism's movable end symmetry is fixed with first drive block and second drive block, first drive block and second drive block all are with first sliding block matched with arc setting, and first drive block and second drive block are concentric setting, first sliding block internally mounted has stop gear, the door opening has been seted up in the box upper end outside, door opening inner wall lower extreme articulates through the hinge has the first door body, box inner wall upper end is fixed with the second baffle, the one end that the door opening is located the second baffle upper surface articulates through the hinge has the second door body, box inner wall middle part is fixed with the installation piece, installation piece middle part is fixed with electron microscope.

Preferably, the sheave reducer comprises a first driving shell, a first rotating shaft, a first driving disk, a shifting groove, an arc-shaped groove, a second rotating shaft, a first servo motor, a second driving disk, a shifting column, a limiting disk and an avoiding groove, the lower surface of the first partition plate is fixed with the first driving shell, the inner wall of the first driving shell is rotatably connected with the first rotating shaft through a bearing, the first rotating shaft is rotatably connected with the first partition plate through a bearing, the top of the first partition plate is fixedly connected with the rotating disk, the bottom of the first rotating shaft is fixed with the first driving disk, the shifting groove and the arc-shaped groove are equidistantly arranged on the outer side of the first driving disk, the inner wall of the first driving shell is rotatably connected with the second rotating shaft through a bearing, the bottom of the first driving shell is fixed with the first servo motor, the output end of the first servo motor is fixedly connected with the second rotating shaft, the middle part of the second rotating shaft is fixed with the second driving disk, the edge of the surface of the second driving disc is fixedly provided with a shifting column, the shifting column is connected with the shifting groove in a sliding mode, one side of the second driving disc is fixedly provided with a limiting disc, the diameter of the limiting disc is the same as that of the arc-shaped groove, and one side of the limiting disc is provided with an avoiding groove.

Preferably, actuating mechanism includes second drive shell, first threaded rod, second sliding block and second servo motor, the one end that first baffle upper surface is close to the door opening is fixed with the second drive shell, second drive shell inner wall is connected with first threaded rod through the bearing rotation, second drive shell inner wall sliding connection has the second sliding block, and first drive block and second drive block all with second sliding block fixed connection, second drive shell one end is fixed with second servo motor, second servo motor's output and first threaded rod fixed connection.

Preferably, the box body is close to one end of the second driving shell and is provided with a material taking hole, the inner wall of the material taking hole is connected with a sliding plate in a sliding mode, the sliding plate is fixedly connected with the second driving block, one end, far away from the second driving block, of the sliding plate is fixedly provided with a sealing door, and the sealing door is arranged in an arc shape matched with the box body.

Preferably, the limiting mechanism comprises a first groove, a second groove, a first sliding hole, a sliding strip, a third sliding block, a trapezoidal block, a second sliding hole, a clamping block, a driving groove, a first spring, a second spring and a clamping groove, the first groove is formed in the inner wall of the first sliding block, the second groove is formed in the top of the inner wall of the first groove, the first sliding hole is formed in one end, close to the first driving block, of the inner wall of the first groove, the sliding strip is connected to the inner wall of the first sliding hole in a sliding mode, the third sliding block is fixed to one end, close to the first groove, of the sliding strip, the trapezoidal block is fixed to the top of the third sliding block, the second sliding hole is formed in one side of the inner wall of the second groove, the clamping block is connected to the inner wall of the second sliding hole in a sliding mode, the lower surface of the clamping block is provided with the driving groove matched with the trapezoidal block, the trapezoidal block is in sliding contact with the inner wall of the driving groove, and the first spring is fixed to one end of the clamping block, and first spring one end and second recess inner wall fixed connection, third sliding block one side symmetry is fixed with the second spring, and second spring one end and first recess inner wall fixed connection, the slide inner wall is close to the one end of fixture block and has seted up the draw-in groove, and fixture block and draw-in groove joint.

Preferably, place a both ends symmetry and be fixed with the spacing piece, the spacing groove has been seted up to slide inner wall symmetry, and spacing piece and spacing groove sliding connection, first recess inner wall is fixed with the guide arm, and the guide arm passes through-hole and third sliding block sliding connection, the guide arm is located a second spring inner wall.

Preferably, LED lamp beads are fixed to the bottom of the inner wall of the placing groove, and a light guide plate is fixed to the inner wall of the placing groove, located at the tops of the LED lamp beads.

Preferably, first servo motor and second servo motor are a gear motor, box one side is fixed with control display panel, and first servo motor, second servo motor, LED lamp pearl and electron microscope all with control display panel electric connection.

Preferably, transparent glass is embedded and fixed in the middle of each of the first door body and the second door body, and handles are fixed on one sides of the first door body and the second door body.

Preferably, threaded sleeves are symmetrically fixed to the bottom of the box body, a second threaded rod is connected to the inner wall of each threaded sleeve in a threaded mode, a hexagonal knob is fixed to the bottom of each second threaded rod, and a placing piece is fixed to the bottom of each hexagonal knob.

Compared with the related technology, the intelligent preparation method of the human stem cell factor skin repair liquid provided by the invention has the following beneficial effects:

the invention provides an intelligent preparation method of a human stem cell factor skin repair liquid, which comprises the following steps:

1. the LED lamp beads and the light guide plate are controlled to emit light, the culture dish is illuminated, the cell condition and the culture medium condition in the culture dish are observed through an electron microscope, the record is carried out, the culture dish on the turntable is driven to rotate by a grooved pulley speed reducer to one station, so that the adjacent culture dish which is not detected rotates to the position below the electron microscope to be observed, the operation is repeated in sequence, the cells in the culture dish can be automatically detected, the frequent detection of workers is avoided, the frequency of opening the first door body is reduced, and the labor intensity of people is reduced;

2. when the culture medium needs to be replaced or the cells need to be diluted is detected, a replacement signal is sent to an operator through the control display panel, the operator controls the first servo motor to rotate through the control display panel, the culture dish needing to be replaced rotates to one end of the sealing door, the first sliding block on the placing block moves to a position between the first driving block and the second driving block, the first driving block is driven to slide through the driving mechanism at the moment, the limiting mechanism is unlocked, the first sliding block is pushed to slide towards one end of the sealing door, the placing block is driven to slide towards one end of the sealing door, the second sliding block can move to drive the sealing door to be far away from the object taking hole through the sliding plate, the sealing door is opened, the culture dish on the placing block is slowly pushed out of the object taking hole, people can directly take out the culture dish, and after the culture dish is taken out, the second servo motor rotates reversely, after the second sliding block is driven to retract, the sealing door is closed to seal the box body, in the process, the culture dish to be removed can be removed only by opening the fetching hole for a period of time, the communication area and time between the box body and the outside are greatly reduced, and air exchange is reduced, so that the temperature, humidity and gas content in the box body can be conveniently and stably controlled, fluctuation is reduced, and energy consumption is reduced;

3. in the process of stretching out and returning the placing block, unlocking and limiting can be automatically carried out, the placing block is prevented from sliding, and stability is improved.

Drawings

FIG. 1 is a schematic view of the overall structure provided by the present invention;

FIG. 2 is a schematic view of the overall internal structure provided by the present invention;

FIG. 3 is a schematic view of a door opening structure provided in the present invention;

FIG. 4 is a schematic view of the fetching hole structure provided by the present invention;

FIG. 5 is a schematic diagram of a placement block structure provided in the present invention;

fig. 6 is a schematic structural view of a placement groove provided by the present invention;

FIG. 7 is a schematic view of a first slider structure according to the present invention;

FIG. 8 is a schematic view of a driving mechanism according to the present invention;

fig. 9 is a schematic structural view of a sheave speed reducer provided in the present invention;

FIG. 10 is a schematic view of a first slide hole structure provided in the present invention;

FIG. 11 is a schematic structural view of a limiting mechanism according to the present invention;

FIG. 12 is a schematic view of a third slide block structure according to the present invention;

FIG. 13 is a schematic diagram of a fixture block structure according to the present invention;

fig. 14 is a schematic structural view of an LED lamp bead provided by the present invention;

FIG. 15 is a flow chart of the preparation process provided by the present invention.

Reference numbers in the figures: 1. a box body; 2. a first separator; 3. a sheave reducer; 31. a first drive case; 32. a first rotating shaft; 33. a first drive disk; 34. a groove is poked; 35. an arc-shaped slot; 36. a second rotating shaft; 37. a first servo motor; 38. a second drive disc; 39. column shifting; 310. a limiting disc; 311. an avoidance groove; 4. a limiting mechanism; 41. a first groove; 42. a second groove; 43. a first slide hole; 44. a slide bar; 45. a third slider; 46. a trapezoidal block; 47. a second slide hole; 48. a clamping block; 49. a drive slot; 410. a first spring; 411. a second spring; 412. a card slot; 5. a drive mechanism; 51. a second drive case; 52. a first threaded rod; 53. a second slider; 54. a second servo motor; 6. a turntable; 7. a sliding groove; 8. placing the blocks; 9. a placement groove; 10. a culture dish; 11. a slideway; 12. a first slider; 13. a first driving block; 14. a second driving block; 15. a door opening; 16. a first door body; 17. a second separator; 18. a second door body; 19. mounting blocks; 20. an electron microscope; 21. taking a hole; 22. a sliding plate; 23. a sealing door; 24. a limiting block; 25. a limiting groove; 26. a guide bar; 27. LED lamp beads; 28. a light guide plate; 29. a control display panel; 101. transparent glass; 102. a handle; 103. a threaded sleeve; 104. a second threaded rod; 105. a hexagonal knob; 106. the sheet is placed.

Detailed Description

The invention is further described with reference to the following figures and embodiments.

In the concrete implementation process, as shown in fig. 1, fig. 2, fig. 3, fig. 4, fig. 5, fig. 6, fig. 7, fig. 8 and fig. 15, an intelligent preparation method of a human stem cell factor skin repair solution is completed by matching an incubator, and the invention is mainly characterized in that the incubator is selected, and in the embodiment, only one preparation method of the product is described, and the preparation method comprises the following steps:

1) collecting umbilical cord blood aseptically, and placing the umbilical cord blood into a culture box for culture by adopting a tissue block direct culture method, wherein the umbilical cord blood collected in a preferred embodiment of the invention is human umbilical cord blood;

wherein, the incubator in the step 1) comprises a box body 1, a first clapboard 2, a sheave reducer 3, a limiting mechanism 4, a driving mechanism 5, a rotary disc 6, a sliding groove 7, a placing block 8, a placing groove 9, a culture dish 10, a slideway 11, a first sliding block 12, a first driving block 13, a second driving block 14, a door opening 15, a first door body 16, a second clapboard 17, a second door body 18, a mounting block 19 and an electron microscope 20, the lower end of the inner wall of the box body 1 is fixed with the first clapboard 2, the lower surface of the first clapboard 2 is fixed with the sheave reducer 3, the output end of the sheave reducer 3 passes through the first clapboard 2 and is fixed with the rotary disc 6, the surface of the rotary disc 6 is equidistantly provided with the sliding groove 7, the inner wall of the sliding groove 7 is slidably connected with the placing block 8, two ends of the placing block 8 are symmetrically fixed with the limiting blocks 24, the inner wall of the slideway 11 is symmetrically provided with the limiting grooves 25, a limiting block 24 is connected with a limiting groove 25 in a sliding manner, a placing groove 9 is arranged on the surface of the placing block 8, a culture dish 10 is placed on the inner wall of the placing groove 9, a slide rail 11 is arranged at the bottom of the inner wall of the sliding groove 7, a first sliding block 12 is fixed at the bottom of the placing block 8, the first sliding block 12 is arranged in an arc shape, a driving mechanism 5 is fixed at one end of the upper surface of the first partition plate 2, a first driving block 13 and a second driving block 14 are symmetrically fixed at the moving end of the driving mechanism 5, the first driving block 13 and the second driving block 14 are both arranged in an arc shape matched with the first sliding block 12 and are concentrically arranged, a limiting mechanism 4 is arranged inside the first sliding block 12, a door opening 15 is arranged on the outer side of the upper end of the box body 1, a first door body 16 is hinged at the lower end of the inner wall of the door opening 15, a second partition plate 17 is fixed at the upper end of the inner wall of the box body 1, one end of the door opening 15, which is positioned on the upper surface of the second partition board 17, is hinged with a second door body 18 through a hinge, an installation block 19 is fixed in the middle of the inner wall of the box body 1, and an electron microscope 20 is fixed in the middle of the installation block 19.

Referring to fig. 7 and 9, the sheave reducer 3 includes a first driving shell 31, a first rotating shaft 32, a first driving disk 33, a shifting groove 34, an arc groove 35, a second rotating shaft 36, a first servo motor 37, a second driving disk 38, a shifting column 39, a limiting disk 310 and an avoiding groove 311, the first driving shell 31 is fixed on the lower surface of the first partition board 2, the inner wall of the first driving shell 31 is rotatably connected with the first rotating shaft 32 through a bearing, the first rotating shaft 32 is rotatably connected with the first partition board 2 through a bearing, the top of the first partition board 2 is fixedly connected with the rotating disk 6, the first driving disk 33 is fixed on the bottom of the first rotating shaft 32, the shifting groove 34 and the arc groove 35 are equidistantly formed on the outer side of the first driving disk 33, the inner wall of the first driving shell 31 is rotatably connected with the second rotating shaft 36 through a bearing, the first servo motor 37 is fixed on the bottom of the first driving shell 31, the output end of the first servo motor 37 is fixedly connected with the second rotating shaft 36, the second driving disk 38 is fixed in the middle of the second rotating shaft 36, the shifting column 39 is fixed on the surface edge of the second driving disk 38, the shifting column 39 is in sliding connection with the shifting groove 34, the limiting disk 310 is fixed on one side of the second driving disk 38, the diameter of the limiting disk 310 is the same as that of the arc-shaped groove 35, the avoiding groove 311 is formed in one side of the limiting disk 310, the second driving disk 38 and the limiting disk 310 are driven to rotate through one rotation of the first servo motor 37, the shifting column 39 is driven to shift the shifting groove 34, the first driving disk 33 rotates one sixth circle, and the principle of the mechanism is the same as that of an existing one sixth sheave mechanism.

Referring to fig. 7 and 8, the driving mechanism 5 includes a second driving shell 51, a first threaded rod 52, a second sliding block 53 and a second servo motor 54, the second driving shell 51 is fixed at one end of the upper surface of the first partition board 2 close to the door opening 15, the inner wall of the second driving shell 51 is rotatably connected with the first threaded rod 52 through a bearing, the second sliding block 53 is slidably connected with the inner wall of the second driving shell 51, the first driving block 13 and the second driving block 14 are both fixedly connected with the second sliding block 53, the second servo motor 54 is fixed at one end of the second driving shell 51, the output end of the second servo motor 54 is fixedly connected with the first threaded rod 52, the second servo motor 54 rotates to drive the threaded rod to rotate, and then the second sliding block 53 can be driven to slide, so as to drive the first driving block 13 to move to one end of the sealing door 23.

Referring to fig. 4 and 7, an object taking hole 21 is formed in one end of the box body 1 close to the second driving shell 51, a sliding plate 22 is connected to an inner wall of the object taking hole 21 in a sliding manner, the sliding plate 22 is fixedly connected with the second driving block 14, a sealing door 23 is fixed to one end of the sliding plate 22 away from the second driving block 14, the sealing door 23 is arranged in an arc shape matched with the box body 1, the sealing door 23 is driven by the driving mechanism 5 to open and close, and the sealing door can be matched with the culture dish 10 on the placing block 8 to open and close.

Referring to fig. 10, 11, 12 and 13, the limiting mechanism 4 includes a first groove 41, a second groove 42, a first sliding hole 43, a sliding bar 44, a third sliding block 45, a trapezoidal block 46, a second sliding hole 47, a locking block 48, a driving groove 49, a first spring 410, a second spring 411 and a locking groove 412, the first groove 41 is formed on the inner wall of the first sliding block 12, the second groove 42 is formed on the top of the inner wall of the first groove 41, the first sliding hole 43 is formed on one end of the inner wall of the first groove 41 close to the first driving block 13, the sliding bar 44 is slidably connected to the inner wall of the first sliding hole 43, the third sliding block 45 is fixed on one end of the sliding bar 44 close to the first groove 41, the trapezoidal block 46 is fixed on the top of the third sliding block 45, the second sliding hole 47 is formed on one side of the inner wall of the second groove 42, the locking block 48 is slidably connected to the inner wall of the second sliding hole 47, the lower surface of the fixture block 48 is provided with a driving groove 49 matched with the trapezoidal block 46, the trapezoidal block 46 is in sliding contact with the inner wall of the driving groove 49, one end of the fixture block 48 is fixed with a first spring 410, one end of the first spring 410 is fixedly connected with the inner wall of the second groove 42, one side of the third sliding block 45 is symmetrically fixed with a second spring 411, one end of the second spring 411 is fixedly connected with the inner wall of the first groove 41, the inner wall of the first groove 41 is fixed with a guide rod 26, the guide rod 26 is in sliding connection with the third sliding block 45 through a through hole, the guide rod 26 is positioned on the inner wall of one second spring 411, one end of the inner wall of the slideway 11, close to the fixture block 48, is provided with a clamping groove 412, the fixture block 48 is clamped with the clamping groove 412, the first driving block 13 is slowly close to the first sliding block 12 and extrudes the sliding strip 44 on the first sliding block 12, so as to extrude the sliding strip 44 and drive the third sliding block 45 to slide, so as to drive the trapezoidal block 46 to slide, so that the inclined surface of the trapezoidal block 46 extrudes the inclined surface of the driving groove 49, and further, the fixture block 48 can be driven to slide slowly into the second groove 42 to be separated from the clamping groove 412, at this time, the first driving block 13 is in contact with the first sliding block 12 and pushes the first sliding block 12 to slide towards one end of the sealing door 23, so as to drive the placing block 8 to slide towards one end of the sealing door 23, and the second sliding block 53 can move to drive the sealing door 23 to be far away from the object taking hole 21 through the sliding plate 22, so as to open the sealing door 23, so that the culture dish 10 on the placing block 8 is pushed out slowly from the object taking hole 21, so that people can take out the culture dish 10 directly, after taking out is completed, the second servo motor 54 rotates reversely to drive the second sliding block 53 to slide backwards, so as to separate the first driving block 13 from the first sliding block 12, and the second driving block 14 is in contact with the first sliding block 12, and the first sliding block 12 is pushed back through the second driving block 14, until the second sliding hole 47 slides to the position coinciding with the slot 412, the fixture block 48 is clamped into the slot 412 under the elastic force of the first spring 410, the placing block 8 can be limited, the placing block 8 is prevented from sliding when the rotating disc 6 rotates, and the second sliding block 53 is retracted to drive the sealing door 23 to close to seal the box body 1.

Referring to fig. 14, LED beads 27 are fixed at the bottom of the inner wall of the placement groove 9, and a light guide plate 28 is fixed on the inner wall of the placement groove 9 at the top of the LED beads 27.

Referring to fig. 1, the first servo motor 37 and the second servo motor 54 are both speed reduction motors, a control display panel 29 is fixed on one side of the box body 1, and the first servo motor 37, the second servo motor 54, the LED lamp beads 27 and the electron microscope 20 are all electrically connected with the control display panel 29.

Referring to fig. 1, transparent glass 101 is embedded and fixed in the middle of each of the first door body 16 and the second door body 18, and a handle 102 is fixed on one side of each of the first door body 16 and the second door body 18.

Referring to fig. 3, threaded sleeves 103 are symmetrically fixed at the bottom of the box body 1, a second threaded rod 104 is connected to the inner wall of the threaded sleeve 103 in a threaded manner, a hexagonal knob 105 is fixed at the bottom of the second threaded rod 104, a placing piece 106 is fixed at the bottom of the hexagonal knob 105, and the hexagonal knob 105 is rotated by a handle to drive the second threaded rod 104 to ascend and descend in the threaded sleeve 103, so that the level of the box body 1 can be adjusted.

The working principle is as follows:

when the cell culture dish is used, a person places the culture dish 10 filled with cells in the placing groove 9, then closes the first door body 16, so that the culture dish 10 is cultured in the box body 1, and the culture dish 10 is illuminated by controlling the LED lamp beads 27 and the light guide plate 28 to emit light, and the cell condition and the culture medium condition in the culture dish 10 are observed through the electron microscope 20, and the cell condition and the culture medium condition are recorded, the first servo motor 37 rotates for one circle to drive the second driving disk 38 and the limiting disk 310 to rotate, and further drive the shifting column 39 to shift the shifting groove 34, so that the first driving disk 33 rotates for one sixth circle, which is the same as the existing one sixth sheave mechanism principle, the first driving disk 33 rotates to drive the first rotating shaft 32 to rotate, and further drive the rotating disk 6 to rotate for one sixth circle, so that the culture dish 10 on the rotating disk 6 rotates for one station, and the adjacent culture dishes 10 which are not detected rotate to the lower part of the electron microscope 20 to observe, the operation is sequentially repeated, when it is detected that the culture medium needs to be replaced or the cells need to be diluted, a replacement signal is sent to the operator through the control display panel 29, the operator controls the first servo motor 37 to rotate through the control display panel 29, the culture dish 10 needing to be replaced is rotated to one end of the sealing door 23, the first sliding block 12 on the placing block 8 is moved to a position between the first driving block 13 and the second driving block 14, at the moment, the second servo motor 54 rotates to drive the threaded rod to rotate, and then the second sliding block 53 can be driven to slide, so that the first driving block 13 is driven to move towards one end of the sealing door 23, the first driving block 13 slowly approaches the first sliding block 12 and extrudes the sliding strip 44 on the first sliding block 12, so that the sliding strip 44 is extruded, the third sliding block 45 is driven to slide, so that the trapezoidal block 46 can be driven to slide, so that the inclined surface of the trapezoidal block 46 extrudes the inclined surface of the driving groove 49, then the fixture block 48 can be driven to slide slowly into the second groove 42 and be separated from the slot 412, at this time, the first driving block 13 contacts with the first sliding block 12 and pushes the first sliding block 12 to slide towards one end of the sealing door 23, so as to drive the placing block 8 to slide towards one end of the sealing door 23, and the second sliding block 53 can move to drive the sealing door 23 to be far away from the object taking hole 21 through the sliding plate 22, so as to open the sealing door 23, so that the culture dish 10 on the placing block 8 is slowly pushed out from the object taking hole 21, people can directly take out the culture dish 10, after the culture dish is taken out, the second servo motor 54 rotates reversely to drive the second sliding block 53 to slide backwards, so as to make the first driving block 13 separated from the first sliding block 12, and the second driving block 14 contacts with the first sliding block 12, the first sliding block 12 is pushed back through the second driving block 14 until the second sliding hole 47 slides to a position coinciding with the slot 412, make fixture block 48 in card income draw-in groove 412 under the elasticity of first spring 410, can carry on spacingly placing piece 8, prevent when carousel 6 is rotatory, it slides to place piece 8, second sliding block 53 withdraws the back, drive sealing door 23 and close, seal box 1, in this process, it only opens a period to get thing hole 21, can get the culture dish 10 of getting rid of, the communicating area and the time of box 1 with the external world that have significantly reduced, reduce the air exchange, thereby make things convenient for humiture and gas content in the stable control box 1, reduce undulant, and reduce the energy consumption.

The circuits and controls involved in the present invention are prior art and will not be described in detail herein.

The above description is only an embodiment of the present invention, and not intended to limit the scope of the present invention, and all modifications of equivalent structures and equivalent processes, which are made by using the contents of the present specification and the accompanying drawings, or directly or indirectly applied to other related technical fields, are included in the scope of the present invention.

Claims

1. An intelligent preparation method of a human stem cell factor skin repair liquid is completed by matching of an incubator, and is characterized by comprising the following steps:

wherein the incubator in the step 1) comprises a box body (1), a first partition plate (2), a grooved pulley speed reducer (3), a limiting mechanism (4), a driving mechanism (5), a turntable (6), a sliding groove (7), a placing block (8), a placing groove (9), a culture dish (10), a slide way (11), a first sliding block (12), a first driving block (13), a second driving block (14), a door opening (15), a first door body (16), a second partition plate (17), a second door body (18), a mounting block (19) and an electron microscope (20), the first partition plate (2) is fixed at the lower end of the inner wall of the box body (1), the grooved pulley speed reducer (3) is fixed on the lower surface of the first partition plate (2), the turntable (6) is fixed at the position where the output end of the grooved pulley (3) penetrates through the first partition plate (2), the sliding grooves (7) are equidistantly formed in the surface of the turntable (6), the inner wall of the sliding groove (7) is connected with a placing block (8) in a sliding manner, the surface of the placing block (8) is provided with a placing groove (9), a culture dish (10) is placed on the inner wall of the placing groove (9), the bottom of the inner wall of the sliding groove (7) is provided with a slide way (11), the bottom of the placing block (8) is fixed with a first sliding block (12), the first sliding block (12) is arranged in an arc shape, one end of the upper surface of the first partition plate (2) is fixed with a driving mechanism (5), the movable end of the driving mechanism (5) is symmetrically fixed with a first driving block (13) and a second driving block (14), the first driving block (13) and the second driving block (14) are both arranged in an arc shape matched with the first sliding block (12), the first driving block (13) and the second driving block (14) are arranged concentrically, and a limiting mechanism (4) is arranged inside the first sliding block (12), door opening (15) have been seted up in the box (1) upper end outside, door opening (15) inner wall lower extreme articulates through the hinge has first door body (16), box (1) inner wall upper end is fixed with second baffle (17), door opening (15) are located the one end of second baffle (17) upper surface and articulate through the hinge has second door body (18), box (1) inner wall middle part is fixed with installation piece (19), installation piece (19) middle part is fixed with electron microscope (20).

2. The intelligent preparation method of human stem cell factor skin repair liquid according to claim 1, wherein the sheave reducer (3) comprises a first driving shell (31), a first rotating shaft (32), a first driving disk (33), a shifting groove (34), an arc-shaped groove (35), a second rotating shaft (36), a first servo motor (37), a second driving disk (38), a shifting column (39), a limiting disk (310) and an avoiding groove (311), the first driving shell (31) is fixed on the lower surface of the first partition plate (2), the first rotating shaft (32) is rotatably connected to the inner wall of the first driving shell (31) through a bearing, the first rotating shaft (32) is rotatably connected to the first partition plate (2) through a bearing, the top of the first partition plate (2) is fixedly connected to the rotating disk (6), the first driving disk (33) is fixed to the bottom of the first rotating shaft (32), first driving-disc (33) outside equidistance has been seted up and has been dialled groove (34) and arc wall (35), first driving-disc (31) inner wall rotates through the bearing and is connected with second pivot (36), first driving-disc (31) bottom is fixed with first servo motor (37), and the output and second pivot (36) fixed connection of first servo motor (37), second pivot (36) middle part is fixed with second driving-disc (38), second driving-disc (38) surface edge is fixed with dials post (39), and dials post (39) and dials groove (34) sliding connection, second driving-disc (38) one side is fixed with spacing dish (310), and spacing dish (310) diameter is the same with arc wall (35) diameter, spacing dish (310) one side has been seted up and has been dodged groove (311).

3. The intelligent preparation method of a human stem cell factor skin repair liquid according to claim 2, characterized in that the driving mechanism (5) comprises a second driving shell (51), a first threaded rod (52), a second sliding block (53) and a second servo motor (54), a second driving shell (51) is fixed at one end of the upper surface of the first clapboard (2) close to the door opening (15), the inner wall of the second driving shell (51) is rotationally connected with a first threaded rod (52) through a bearing, the inner wall of the second driving shell (51) is connected with a second sliding block (53) in a sliding way, and the first driving block (13) and the second driving block (14) are both fixedly connected with the second sliding block (53), a second servo motor (54) is fixed at one end of the second driving shell (51), the output end of the second servo motor (54) is fixedly connected with the first threaded rod (52).

4. The intelligent preparation method of the human stem cell factor skin repair liquid according to claim 3, wherein an object taking hole (21) is formed in one end of the box body (1) close to the second driving shell (51), a sliding plate (22) is connected to the inner wall of the object taking hole (21) in a sliding manner, the sliding plate (22) is fixedly connected with the second driving block (14), a sealing door (23) is fixed to one end, far away from the second driving block (14), of the sliding plate (22), and the sealing door (23) is arranged in an arc shape matched with the box body (1).

5. The intelligent preparation method of the human stem cell factor skin repair liquid according to claim 4, wherein the limiting mechanism (4) comprises a first groove (41), a second groove (42), a first sliding hole (43), a sliding bar (44), a third sliding block (45), a trapezoidal block (46), a second sliding hole (47), a clamping block (48), a driving groove (49), a first spring (410), a second spring (411) and a clamping groove (412), the first groove (41) is formed in the inner wall of the first sliding block (12), the second groove (42) is formed in the top of the inner wall of the first groove (41), the first sliding hole (43) is formed in one end, close to the first driving block (13), of the inner wall of the first groove (41), the sliding bar (44) is connected with the sliding bar (44) in a sliding manner, the third sliding block (45) is fixed at one end, close to the first groove (41), of the sliding bar (44), a trapezoidal block (46) is fixed at the top of the third sliding block (45), a second sliding hole (47) is formed in one side of the inner wall of the second groove (42), a clamping block (48) is connected to the inner wall of the second sliding hole (47) in a sliding manner, a driving groove (49) matched with the trapezoidal block (46) is formed in the lower surface of the clamping block (48), the trapezoid block (46) is in sliding contact with the inner wall of the driving groove (49), one end of the clamping block (48) is fixed with a first spring (410), and one end of the first spring (410) is fixedly connected with the inner wall of the second groove (42), a second spring (411) is symmetrically fixed on one side of the third sliding block (45), and one end of the second spring (411) is fixedly connected with the inner wall of the first groove (41), a clamping groove (412) is formed in one end, close to the clamping block (48), of the inner wall of the slide rail (11), and the clamping block (48) is clamped with the clamping groove (412).

6. The intelligent preparation method of the human stem cell factor skin repair liquid according to claim 5, wherein limiting blocks (24) are symmetrically fixed to two ends of the placing block (8), limiting grooves (25) are symmetrically formed in the inner wall of the slide way (11), the limiting blocks (24) are slidably connected with the limiting grooves (25), guide rods (26) are fixed to the inner wall of the first groove (41), the guide rods (26) are slidably connected with the third sliding block (45) through holes, and the guide rods (26) are located on the inner wall of one second spring (411).

7. The intelligent preparation method of the human stem cell factor skin repair liquid according to claim 3, wherein LED beads (27) are fixed to the bottom of the inner wall of the placement groove (9), and a light guide plate (28) is fixed to the inner wall of the placement groove (9) on the top of the LED beads (27).

8. The intelligent preparation method of the human stem cell factor skin repair liquid according to claim 7, wherein the first servo motor (37) and the second servo motor (54) are both deceleration motors, a control display panel (29) is fixed on one side of the box body (1), and the first servo motor (37), the second servo motor (54), the LED lamp bead (27) and the electron microscope (20) are all electrically connected with the control display panel (29).

9. The intelligent preparation method of the human stem cell factor skin repair liquid according to claim 1, wherein transparent glass (101) is embedded and fixed in the middle of each of the first door body (16) and the second door body (18), and a handle (102) is fixed on one side of each of the first door body (16) and the second door body (18).

10. The intelligent preparation method of the human stem cell factor skin repair liquid according to claim 1, wherein a threaded sleeve (103) is symmetrically fixed at the bottom of the box body (1), a second threaded rod (104) is connected to the inner wall of the threaded sleeve (103) in a threaded manner, a hexagonal knob (105) is fixed at the bottom of the second threaded rod (104), and a placing sheet (106) is fixed at the bottom of the hexagonal knob (105).