CN208632566U - A kind of floated cell culture apparatus - Google Patents
A kind of floated cell culture apparatus Download PDFInfo
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- CN208632566U CN208632566U CN201820881133.8U CN201820881133U CN208632566U CN 208632566 U CN208632566 U CN 208632566U CN 201820881133 U CN201820881133 U CN 201820881133U CN 208632566 U CN208632566 U CN 208632566U
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- China
- Prior art keywords
- pedestal
- cell culture
- lid
- culture apparatus
- rabbling mechanism
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Abstract
The utility model provides a kind of floated cell culture apparatus, including device noumenon, pedestal, rubber suction cups, motor, rabbling mechanism, gas injection torus, snorkel, lid, sealing ring, screw thread coupling, U-tube, sampling mechanism, support sleeve, gas detecting instrument and temperature monitor, the lower part of described device ontology is equipped with pedestal, the lower part of the pedestal is equipped with rubber suction cups, motor is equipped in the middle part of the inner cavity of the pedestal, the upper end of the motor is connected with rabbling mechanism, the rabbling mechanism is located at the intracavity bottom of device noumenon, the periphery of the rabbling mechanism is equipped with gas injection torus.The lower port of extraction conduit is flapped toward different directions by swing rotary sphere by the setting of the sampling mechanism of the utility model, can do sample analysis by the culture solution of different location in draw-out device chamber body as needed.
Description
Technical field
The utility model belongs to technical field of cell culture more particularly to a kind of floated cell culture apparatus.
Background technique
Suspension cell culture device refer to it is a kind of in the fluid nutrient medium for constantly being stirred or being shaken, cultivate it is slender
The culture apparatus of born of the same parents and small cell cluster is a kind of training method of non-anchorage-dependent cell.Certain anchorage-dependent cell warps
The method culture can also be used by crossing adaptation and selection.It is relatively simple to increase suspension culture scale, as long as increasing volume
?.Depth is more than 5mm, needs to stir culture medium, more than 10cm, it is also necessary to which deep layer is passed through CO2 and air, to guarantee enough gas
Body exchange.Cell is set to be in the cultural method that dispersion is suspended in culture solution always by oscillation or rotating device.
It has widely been answered in genetic experiment using the method that suspension cell culture device cultivates the cells
With, but suspension cell culture device structure currently on the market is relatively simple, and culture speed is slower, and the effect cultivated compared with
Difference.Wherein, probe tube is fixed in the position of culture apparatus body interior, cannot arbitrarily take the liquid sample of multiple positions.
Therefore, a kind of floated cell culture apparatus is invented to be very necessary.
Utility model content
In order to solve the above-mentioned technical problem, the utility model provides a kind of floated cell culture apparatus, existing to solve
Suspension cell culture device culture speed is slow, and effect is poor, the fixed problem in sample position.A kind of floated cell culture apparatus,
Including device noumenon, pedestal, rubber suction cups, motor, rabbling mechanism, gas injection torus, snorkel, lid, sealing ring, screw thread company
Device, U-tube, sampling mechanism, support sleeve, gas detecting instrument and temperature monitor are connect, the lower part of described device ontology is equipped with pedestal,
The lower part of the pedestal is equipped with rubber suction cups, is equipped with motor in the middle part of the inner cavity of the pedestal, and the upper end of the motor is connected with rabbling mechanism,
The rabbling mechanism is located at the intracavity bottom of device noumenon, and the periphery of the rabbling mechanism is equipped with gas injection torus, the gas injection torus
Upper end two sides are connected with snorkel, and lid, the lid and device noumenon top edge nested encryptions are run through in the upper end of snorkel, and
Pad is equipped with sealing ring；The snorkel includes air inlet pipe and an air outlet pipe, and the air inlet pipe and an air outlet pipe is located at the two sides of lid,
The upper end of middle escape pipe is connected through a screw thread device and is connected with U-tube；The middle part of the lid is equipped with stepped groove, and is equipped in slot
Sampling mechanism.
The gas injection torus includes annulus main body, venthole and connectivity port, is densely covered with outlet in the annulus main body
Hole, and connectivity port is equipped in the upper end two sides of annulus main body.
The sampling mechanism includes extraction conduit, spinning ball and draw-off pump, and the extraction conduit runs through spinning ball, and
It is fixedly connected, the upper end of the extraction conduit is equipped with draw-off pump.
The spinning ball is located in the stepped groove being equipped in the middle part of lid, and is equipped with support sleeve on the periphery of slot and is used for rotation
The support for turning sphere is conducive to flexibly support spinning ball by support sleeve, reduces vibration and plays dust-proof work simultaneously
With.
Gas detecting instrument is installed in described device chamber body, is conducive to detect the carbon dioxide or air being passed through
Ingredient and concentration.
The lower part of described device chamber body is equipped with temperature monitor, is conducive to the temperature of real-time detection cell culture fluid
Degree, guarantees that cell is in required optimum culturing temperature.
Compared with prior art, the utility model has the following beneficial effects:
1. the setting of the rubber suction cups of the utility model plays securing device ontology in rabbling mechanism work
Effect, is conducive to buffering vibration and noise-decreasing.
2. the setting of the U-tube of the utility model, change the direction of outlet pipe port, make opening down, plays
Dust-proof effect advantageously ensures that the sanitation and hygiene of device noumenon inner cavity.
3. the setting of the sampling mechanism of the utility model, by swing rotary sphere, by the lower port of extraction conduit
It is flapped toward different directions, sample can be made by the culture solution of different location in draw-out device chamber body as needed
Analysis.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of the utility model.
Fig. 2 is the structural schematic diagram of the gas injection torus of the utility model.
Fig. 3 is the structural schematic diagram of the sampling mechanism of the utility model.
In figure:
1- device noumenon, 2- pedestal, 3- rubber suction cups, 4- motor, 5- rabbling mechanism, 6- gas injection torus, 61- annulus master
Body, 62- venthole, the connectivity port 63-, 7- snorkel, 71- air inlet pipe, 72- escape pipe, 8- lid, 9- sealing ring, 10- screw thread
Connector, 11-U shape pipe, 12- sampling mechanism, 121- extraction conduit, 122- spinning ball, 123- draw-off pump, 13- support sleeve,
14- gas detecting instrument, 15- temperature monitor.
Specific embodiment
The utility model is described further below in conjunction with attached drawing:
Embodiment:
As shown in attached drawing 1 to attached drawing 3
The utility model provides a kind of floated cell culture apparatus, including device noumenon 1, pedestal 2, rubber suction cups 3, electricity
Machine 4, rabbling mechanism 5, gas injection torus 6, snorkel 7, lid 8, sealing ring 9, screw thread coupling 10, U-tube 11, sampling mechanism
12, support sleeve 13, gas detecting instrument 14 and temperature monitor 15, the lower part of described device ontology 1 are equipped with pedestal 2, the pedestal 2
Lower part is equipped with rubber suction cups 3, is equipped with motor 4 in the middle part of the inner cavity of the pedestal 2, and the upper end of the motor 4 is connected with rabbling mechanism 5, should
Rabbling mechanism 5 is located at the intracavity bottom of device noumenon 1, and the periphery of the rabbling mechanism 5 is equipped with gas injection torus 6, the gas injection annulus
6 upper end two sides of body are connected with snorkel 7, and lid 8 is run through in the upper end of snorkel 7, and the lid 8 and 1 top edge of device noumenon are embedding
Set connection, and pad and be equipped with sealing ring 9；The snorkel 7 includes air inlet pipe 71 and escape pipe 72, the air inlet pipe 71 and escape pipe
72 are located at the two sides of lid 8, and wherein the upper end of escape pipe 72 is connected through a screw thread device 10 and is connected with U-tube 11；The lid 8
Middle part is equipped with stepped groove, and sampling mechanism 12 is equipped in slot.
The gas injection torus 6 includes annulus main body 61, venthole 62 and connectivity port 63, close in the annulus main body 61
It is furnished with venthole 62, and is equipped with connectivity port 63 in the upper end two sides of annulus main body 61.
The sampling mechanism 12 includes extraction conduit 121, spinning ball 122 and draw-off pump 123, the extraction conduit 121
It through spinning ball 122, and is fixedly connected, the upper end of the extraction conduit 121 is equipped with draw-off pump 123.
The spinning ball 122 is located in the stepped groove being equipped in the middle part of lid 8, and uses on the periphery of slot equipped with support sleeve 13
In the support to spinning ball 122, be conducive to flexibly support spinning ball 122 by support sleeve 13, reduce vibration and
Dustproof effect is played simultaneously.
Gas detecting instrument 14 is installed on 1 inner cavity of described device ontology, is conducive to carbon dioxide or sky that detection is passed through
The ingredient and concentration of gas.
The lower part of 1 inner cavity of described device ontology is equipped with temperature monitor 15, is conducive to real-time detection cell culture fluid
Temperature guarantees that cell is in required optimum culturing temperature.
Working principle
In the utility model, pedestal is fixed to the working face together with device noumenon by rubber suction cups, in rabbling mechanism
When work, plays the role of securing device ontology, be conducive to buffering vibration and noise-decreasing.By swing rotary sphere, can incite somebody to action
The lower port of sampling extraction conduit is flapped toward different directions, as needed can in draw-out device chamber body different location culture
Liquid does sample analysis.
Using technical solution described in the utility model or those skilled in the art in technical solutions of the utility model
Under inspiration, similar technical solution is designed, and reaches above-mentioned technical effect, is the protection model for falling into the utility model
It encloses.
Claims (6)
1. a kind of floated cell culture apparatus, it is characterised in that: including device noumenon (1), pedestal (2), rubber suction cups (3),
Motor (4), rabbling mechanism (5), gas injection torus (6), snorkel (7), lid (8), sealing ring (9), screw thread coupling (10),
U-tube (11), sampling mechanism (12), support sleeve (13), gas detecting instrument (14) and temperature monitor (15), described device ontology
(1) lower part is equipped with pedestal (2), and the lower part of the pedestal (2) is equipped with rubber suction cups (3), and electricity is equipped in the middle part of the inner cavity of the pedestal (2)
Machine (4), the upper end of the motor (4) are connected with rabbling mechanism (5), which is located at the interior bottom of chamber of device noumenon (1)
The periphery in portion, the rabbling mechanism (5) is equipped with gas injection torus (6), which is connected with snorkel
(7), and lid (8) are run through in the upper end of snorkel (7), the lid (8) and device noumenon (1) top edge nested encryptions, and pad is set
There are sealing ring (9)；The snorkel (7) includes air inlet pipe (71) and escape pipe (72), the air inlet pipe (71) and escape pipe
(72) it is located at the two sides of lid (8), wherein the upper end of escape pipe (72) is connected through a screw thread device (10) and is connected with U-tube (11)；
The middle part of the lid (8) is equipped with stepped groove, and is equipped with sampling mechanism (12) in slot.
2. floated cell culture apparatus as described in claim 1, it is characterised in that: the gas injection torus (6) includes circle
Ring main body (61), venthole (62) and connectivity port (63) are densely covered with venthole (62) on the annulus main body (61), and in circle
The upper end two sides of ring main body (61) are equipped with connectivity port (63).
3. floated cell culture apparatus as described in claim 1, it is characterised in that: the sampling mechanism (12) includes extracting
Pipeline (121), spinning ball (122) and draw-off pump (123), the extraction conduit (121) run through spinning ball (122), and solid
The upper end of fixed connection, the extraction conduit (121) is equipped with draw-off pump (123).
4. floated cell culture apparatus as claimed in claim 3, it is characterised in that: the spinning ball (122) is located at lid
In the stepped groove being equipped in the middle part of body (8), and support sleeve (13) are equipped with for the support to spinning ball (122) on the periphery of slot.
5. floated cell culture apparatus as described in claim 1, it is characterised in that: described device ontology (1) inner cavity it is upper
Portion is equipped with gas detecting instrument (14).
6. floated cell culture apparatus as described in claim 1, it is characterised in that: under described device ontology (1) inner cavity
Portion is equipped with temperature monitor (15).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820881133.8U CN208632566U (en) | 2018-06-08 | 2018-06-08 | A kind of floated cell culture apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820881133.8U CN208632566U (en) | 2018-06-08 | 2018-06-08 | A kind of floated cell culture apparatus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208632566U true CN208632566U (en) | 2019-03-22 |
Family
ID=65733423
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201820881133.8U Active CN208632566U (en) | 2018-06-08 | 2018-06-08 | A kind of floated cell culture apparatus |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN208632566U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114379920A (en) * | 2022-01-27 | 2022-04-22 | 抚州三和医药化工有限公司 | Transfer device of omeprazole production usefulness |
-
2018
- 2018-06-08 CN CN201820881133.8U patent/CN208632566U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114379920A (en) * | 2022-01-27 | 2022-04-22 | 抚州三和医药化工有限公司 | Transfer device of omeprazole production usefulness |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20190513 Address after: 264000 Qiujiazhuang Village, Menlou Town, Fushan District, Yantai City, Shandong Province Patentee after: Shandong Weiheng Biotechnology Co., Ltd. Address before: 150000 Ma Duan Street, Nangang District, Harbin City, Heilongjiang Province Patentee before: Zhao Guangtao |
|
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20211216 Address after: 264000 East Electronic Industry Institute, Chaoshui Wenhua Road, Yantai Economic and Technological Development Zone, Shandong Province Patentee after: SHANDONG BAIJUN BIOTECHNOLOGY CO.,LTD. Address before: 264000 Qiujiazhuang Village, Menlou Town, Fushan District, Yantai City, Shandong Province Patentee before: SHANDONG WEIHENG BIOLOGICAL TECHNOLOGY Co.,Ltd. |