CN218969251U

CN218969251U - Cell culture device capable of detecting cell culture solution

Info

Publication number: CN218969251U
Application number: CN202223433825.1U
Authority: CN
Inventors: 董宝莲; 郭玲; 宋超; 何柯欣; 双盈波
Original assignee: Anning First People's Hospital
Current assignee: Anning First People's Hospital
Priority date: 2022-12-21
Filing date: 2022-12-21
Publication date: 2023-05-05
Anticipated expiration: 2032-12-21

Abstract

The application belongs to the technical field of cell culture, a cell culture apparatus that can detect cell culture liquid is disclosed, which comprises a main body, one side fixedly connected with connection valve of main part, one side fixedly connected with coupling hose of connection valve, coupling hose's tip swing joint has the sample dish, the opposite side fixedly connected with connecting catheter of main part, connecting catheter's tip fixedly connected with steady voltage mechanism, the fixed mounting ring has been cup jointed to the fixed surface of main part, fixed mounting ring's surface outside fixedly connected with connection handle, through connecting valve, connecting catheter and steady voltage mechanism that is equipped with, be convenient for draw the inside cell liquid of main part in adopting the negative pressure, can reduce the inside volume of main part and steady voltage mechanism and maintain the pressure of main part inside through steady voltage mechanism to can cultivate the cell steadily.

Description

Cell culture device capable of detecting cell culture solution

Technical Field

The present application relates to the field of cell culture technology, and more particularly, to a cell culture apparatus capable of detecting a cell culture solution.

Background

Cell culture refers to a method of simulating in vitro an in vivo environment (sterile, suitable temperature, ph, certain nutritional conditions, etc.) to survive, grow, reproduce and maintain major structures and functions. Cell culture is also known as cell cloning technology, a formal biological term being cell culture technology. Cell culture is an essential process for the whole biotechnology, or for one of the biological cloning techniques, and is itself a large-scale cloning of cells. The cell culture technique can be a simple single cell or a few differentiated multicellular from a single cell through mass culture, which is an essential link of the cloning technique, and the cell culture itself is the cloning of the cell. Cell culture techniques are important and commonly used techniques in cell biology research methods, by which a large number of cells can be obtained, and by which signal transduction of cells, anabolism of cells, growth and proliferation of cells, etc. can be studied.

Through retrieving, as the cell culture device of cell culture liquid environment can be monitored that publication number CN207405183U discloses, wherein mainly, take out the air in the sampling dish through the negative pressure air pump, reduce the pressure in the sampling dish, under the inside and outside pressure differential effect of sampling dish, in carrying the sampling dish with the cell culture liquid in the culture dish through the hose, wherein the dish lid passes through screw structure movable mounting on the dish body, through the dismantlement between the dish body and the dish lid, the convenience is taken a sample the cell culture liquid in the sampling dish and is detected, thereby avoid directly opening the culture dish and the balanced environment imbalance in the culture dish that causes.

In the above technical scheme, through reducing the pressure in the sampling dish and promoting the cell liquid in the culture dish to flow to the inside of sampling dish through the hose, this operation can be in the twinkling of an eye reduce the pressure in the culture dish, can cause certain influence to cell culture, if follow-up when supplementing so that maintain the pressure, then still can change the great environment in the culture dish, still can cause the influence to cell culture for the testing result is inaccurate, for this, we propose a cell culture device that can detect cell culture liquid.

Disclosure of Invention

In order to solve the above problems, the present application provides a cell culture apparatus capable of detecting a cell culture liquid.

The application provides a cell culture device that can detect cell culture liquid adopts following technical scheme:

the utility model provides a cell culture device that can detect cell culture liquid, includes the main part, one side fixedly connected with connection valve of main part, one side fixedly connected with coupling hose of connection valve, coupling hose's tip swing joint has the sample dish, the opposite side fixedly connected with connecting catheter of main part, the tip fixedly connected with steady voltage mechanism of connecting catheter.

Further, the top fixedly connected with of main part connects transparent top cap, the top fixedly connected with fixed locating lever of transparent top cap, the activity cup joints movable cover dish outside the surface of fixed locating lever.

Through above-mentioned technical scheme, deflect in the surface outside of fixed locating lever through movable cover dish can be according to the cultivation in-process of cell to the luminousness of connecting transparent top cap is adjusted as required.

Further, the fixed mounting ring is fixedly sleeved on the surface of the main body, and the connecting handle is fixedly connected to the outer side of the surface of the fixed mounting ring.

Through above-mentioned technical scheme, can be steady drive the main part wholly remove through the effect of connecting handle and fixed mounting ring, be convenient for shift.

Further, the top fixedly connected with fixed limiting plate of fixed locating lever.

Through above-mentioned technical scheme, can restrict the position of activity bent cap through fixed limiting disc, prevent that the activity bent cap from breaking away from the surface outside of fixed locating lever.

Further, the voltage stabilizing mechanism comprises a fixed sleeve, a movable piston is movably connected in the fixed sleeve, one side of the movable piston is fixedly connected with a connecting push-pull rod, and one end of the connecting push-pull rod is fixedly connected with a connecting push-pull block.

Through the technical scheme, the push-pull rod and the movable piston are driven to move by pushing the connecting push-pull block, so that the volume is reduced to restore the pressure.

Further, a transparent observation window is arranged on the surface of the fixed sleeve.

Through above-mentioned technical scheme, can observe the position of movable piston at any time through the transparent observation window on fixed sleeve surface.

In summary, the present application includes at least one of the following beneficial technical effects:

(1) The connecting valve, the connecting conduit and the pressure stabilizing mechanism are arranged, so that the cell fluid in the main body can be conveniently extracted by adopting negative pressure, and the volumes in the main body and the pressure stabilizing mechanism can be reduced by the action of the pressure stabilizing mechanism to maintain the pressure in the main body, so that the cells can be stably cultured;

(2) Through the transparent top cap of connection that is equipped with, fixed locating lever and activity bent cap dish, the activity bent cap dish of being convenient for can deflect in the surface outside of fixed locating lever, can shelter from the transparent top cap of connection to can change the luminousness of connecting transparent top cap, adjust at any time according to at cell culture's in-process.

Drawings

FIG. 1 is a schematic diagram of a cell culture apparatus capable of detecting a cell culture solution;

FIG. 2 is a schematic diagram of a voltage stabilizing mechanism according to the present application;

fig. 3 is an enlarged schematic view of the structure at a in fig. 1 of the present application.

The reference numerals in the figures illustrate:

1. a main body; 2. connecting a valve; 3. a connecting hose; 4. sampling vessel; 5. a connecting conduit; 6. a voltage stabilizing mechanism; 7. connecting a transparent top cover; 8. fixing a positioning rod; 9. a movable cover plate; 10. fixing the mounting ring; 11. a connecting handle; 12. fixing a limiting disc; 13. a fixed sleeve; 14. a movable piston; 15. the push-pull rod is connected; 16. and the push-pull block is connected.

Detailed Description

The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application; it is apparent that the described embodiments are only a part of the embodiments of the present application, not all of the embodiments, and all other embodiments obtained by a person having ordinary skill in the art without making creative efforts based on the embodiments in the present application are within the scope of protection of the present application.

In the description of the present application, it should be noted that the directions or positional relationships indicated by the terms "upper", "lower", "inner", "outer", "top/bottom", etc. are based on the directions or positional relationships shown in the drawings, are merely for convenience of description of the present application and to simplify the description, and do not indicate or imply that the devices or elements to be referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present application. Furthermore, the terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

In the description of the present application, it should be noted that, unless explicitly specified and limited otherwise, the terms "mounted," "configured to," "engaged with," "connected to," and the like are to be construed broadly, and may be either fixedly connected, detachably connected, or integrally connected, for example; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the terms in this application will be understood by those of ordinary skill in the art in a specific context.

Example 1:

the present application is described in further detail below in conjunction with figures 1-3.

The embodiment of the application discloses cell culture device that can detect cell culture liquid please refer to fig. 1-3, including main part 1, one side fixedly connected with connection valve 2 of main part 1, one side fixedly connected with coupling hose 3 of connection valve 2, the tip swing joint of coupling hose 3 has sample dish 4, the opposite side fixedly connected with connecting catheter 5 of main part 1, the tip fixedly connected with constant voltage mechanism 6 of connecting catheter 5.

Referring to fig. 3, a connecting transparent top cover 7 is fixedly connected to the top of the main body 1, a fixed positioning rod 8 is fixedly connected to the top of the connecting transparent top cover 7, and a movable cover plate 9 is movably sleeved on the outer side of the surface of the fixed positioning rod 8.

Referring to fig. 1 and 2, a fixed mounting ring 10 is fixedly sleeved on the surface of a main body 1, a connecting handle 11 is fixedly connected to the outer side of the surface of the fixed mounting ring 10, a voltage stabilizing mechanism 6 comprises a fixed sleeve 13, a movable piston 14 is movably connected to the inside of the fixed sleeve 13, a connecting push-pull rod 15 is fixedly connected to one side of the movable piston 14, a connecting push-pull block 16 is fixedly connected to one end of the connecting push-pull rod 15, and a transparent observation window is arranged on the surface of the fixed sleeve 13.

Referring to fig. 3, a fixed limiting plate 12 is fixedly connected to the top of the fixed positioning rod 8.

The implementation principle of the cell culture device capable of detecting the cell culture solution in the embodiment of the application is as follows: in the in-process of using, can open connecting valve 2, if sample dish 4 produces the negative pressure through the negative pressure air pump, the inside cell liquid of main part 1 can flow to the inside of sample dish 4 through connecting valve 2 and coupling hose 3, movable piston 14 moves in the inside of fixed sleeve 13 simultaneously, balance the inside pressure of main part 1 through reducing the volume, if when not adopting the negative pressure jar, then can drive connecting push-and-pull rod 15 and movable piston 14 through the push-and-pull piece 16 of push-and-pull connection, make movable piston 14 can remove in the inboard of fixed sleeve 13, with the inside of cell liquid through connecting valve 2 and coupling hose 3 driving into sample dish 4, close connecting valve 2 after the sample is finished, conveniently change the inside environment of main part 1, thereby can detect the inside cell liquid of sample dish 4, deflect in the surface outside of fixed positioning rod 8 through movable cover disk 9, can make movable cover disk 9 can be covered at the top of connecting transparent top cap 7, can adjust the luminousness, can adjust at any time according to the in-process of cell culture.

The foregoing are all preferred embodiments of the present application, and are not intended to limit the scope of the present application in any way, therefore: all equivalent changes in structure, shape and principle of this application should be covered in the protection scope of this application.

Claims

1. Cell culture apparatus capable of detecting cell culture fluid, comprising a main body (1), characterized in that: one side fixedly connected with connection valve (2) of main part (1), one side fixedly connected with coupling hose (3) of connection valve (2), the tip swing joint of coupling hose (3) has sample dish (4), the opposite side fixedly connected with connecting catheter (5) of main part (1), the tip fixedly connected with steady voltage mechanism (6) of connecting catheter (5).

2. The cell culture apparatus of claim 1, wherein the cell culture fluid is detected by: the top fixedly connected with of main part (1) connects transparent top cap (7), the top fixedly connected with of connecting transparent top cap (7) fixes locating lever (8), movable lid dish (9) have been cup jointed in the activity of the surface outside of fixed locating lever (8).

3. The cell culture apparatus of claim 1, wherein the cell culture fluid is detected by: the surface fixing sleeve of the main body (1) is fixedly sleeved with a fixing mounting ring (10), and the outer side of the surface of the fixing mounting ring (10) is fixedly connected with a connecting handle (11).

4. A cell culture apparatus according to claim 2, wherein the cell culture fluid is detected by: the top of the fixed positioning rod (8) is fixedly connected with a fixed limiting disc (12).

5. The cell culture apparatus of claim 1, wherein the cell culture fluid is detected by: the pressure stabilizing mechanism (6) comprises a fixed sleeve (13), a movable piston (14) is movably connected inside the fixed sleeve (13), one side of the movable piston (14) is fixedly connected with a connecting push-pull rod (15), and one end of the connecting push-pull rod (15) is fixedly connected with a connecting push-pull block (16).

6. The cell culture apparatus according to claim 5, wherein the cell culture fluid is detected by: the surface of the fixed sleeve (13) is provided with a transparent observation window.