CN206512225U - A kind of plating cells device using magnetic liquid as medium - Google Patents
A kind of plating cells device using magnetic liquid as medium Download PDFInfo
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- CN206512225U CN206512225U CN201720054665.XU CN201720054665U CN206512225U CN 206512225 U CN206512225 U CN 206512225U CN 201720054665 U CN201720054665 U CN 201720054665U CN 206512225 U CN206512225 U CN 206512225U
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- Prior art keywords
- micro
- coil
- permanent magnet
- medium
- magnetic liquid
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- 239000007788 liquid Substances 0.000 title claims abstract description 26
- 238000007747 plating Methods 0.000 title claims abstract description 19
- 229910052710 silicon Inorganic materials 0.000 claims abstract description 18
- 239000010703 silicon Substances 0.000 claims abstract description 18
- 239000000758 substrate Substances 0.000 claims abstract description 18
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 claims abstract description 17
- 238000007789 sealing Methods 0.000 claims abstract description 4
- 239000004020 conductor Substances 0.000 claims description 26
- 238000000926 separation method Methods 0.000 claims description 17
- 230000005415 magnetization Effects 0.000 claims description 4
- YTCQFLFGFXZUSN-BAQGIRSFSA-N microline Chemical compound OC12OC3(C)COC2(O)C(C(/Cl)=C/C)=CC(=O)C21C3C2 YTCQFLFGFXZUSN-BAQGIRSFSA-N 0.000 claims description 3
- 238000000034 method Methods 0.000 abstract description 6
- 210000004027 cell Anatomy 0.000 description 42
- 230000000694 effects Effects 0.000 description 3
- 239000011553 magnetic fluid Substances 0.000 description 3
- 230000009087 cell motility Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 230000033001 locomotion Effects 0.000 description 2
- 239000006249 magnetic particle Substances 0.000 description 2
- 238000001259 photo etching Methods 0.000 description 2
- 230000001464 adherent effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 230000030570 cellular localization Effects 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000005426 magnetic field effect Effects 0.000 description 1
- 230000005389 magnetism Effects 0.000 description 1
- 210000004962 mammalian cell Anatomy 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
Landscapes
- Water Treatment By Electricity Or Magnetism (AREA)
Abstract
The utility model is related to a kind of plating cells device using magnetic liquid as medium, belongs to biomedical engineering field.Device described in the utility model includes silicon chip substrate, T-shaped passage, micro-coil I, micro-coil II, permanent magnet I, permanent magnet II, Programmable AC Power Source, silicon chip substrate is provided with micro-coil I and micro-coil II, micro-coil I and micro-coil II point-blank, and leave gap between the two；The sealing of T-shaped passage is fixed in silicon chip substrate, and micro-coil I and micro-coil II are located at below the horizontal channel of T-shaped passage, and the gap and the Vertical Channel of T-shaped passage between micro-coil I and micro-coil II are just right；Transparent cover plate is provided with above T-shaped passage, cover plate is provided with permanent magnet I or permanent magnet II, and permanent magnet I or permanent magnet II are located at the surface in the gap between micro-coil I and micro-coil II；Micro-coil I, each wire of micro-coil II are all connected with a Programmable AC Power Source.The utility model can effectively reduce the perturbed problem in artificial operating process, improve the efficiency of plating of cell.
Description
Technical field
The utility model is related to biomedical engineering field, more particularly to a kind of plating cells using magnetic liquid as medium
Device.
Background technology
The in vitro culture of cell and tissue is the important means of life science.Some cells and tissue can be in suspensions
Grown under state, but quite a few mammalian cell needs progress surface adherent.Therefore, for providing cell injuring model ring
Blake bottle, culture plate and the culture dish in border except with good transparency and it is nontoxic, sterile in addition to, also need to enable cell to paste
Wall, division and growth.But, among practical application, due to cell volume and quality very little, extraneous disturbance somewhat will be right
The motion of cell produces significant impact.And plating cells inequality can then cause the problems such as cell growth state is not good, cause reality
The failure tested.
At present, in order to reach the purpose for allowing cell to be uniformly distributed on culture vessel wall, the method generally used is to draw
" ten " word, which intersects, gently to be mixed；During inoculating cell liquid, it is aligned in the middle of culture plate and is added with pipette tips, allow cell suspension voluntarily to flow through entirely
Hole, and the volume of the cell liquid of addition need to be strictly held, it is noted that holding level with both hands and handling with care during mobile culture vessel.These behaviour
Make requirement to experimenter higher, with more subjectivity, it is desirable to have rich experience, known technical ability, slightly carelessness or
Careless manipulation will cause the failure of an experiment.Therefore, obtaining fast and convenient and reproducible method makes cell uniform in bed board
It is affixed on and improving and development for cell culture technology is may advantageously facilitate on culture vessel wall, tool is of great significance.
The soliquid that magnetic liquid is made up of nano magnetic particle, base load liquid and the part of surfactant three, be
Have the functional fluid of magnetic and mobility concurrently.Because magnetic liquid can be to non-magnetic particle therein under additional magnetic fields
A magnetic buoyancy is produced, because of this peculiar property so as to be used to realize cell separation, sieve in many scientific research fields
Choosing and cell capture etc..
The content of the invention
In order to overcome the shortcoming of prior art, the purpose of this utility model is that providing one kind uses magnetic liquid as matchmaker
The plating cells device of Jie, should have the advantages that cost was low, efficiency high.
In order to achieve the above object, the technical solution adopted in the utility model is：It is a kind of using magnetic liquid as the thin of medium
Born of the same parents' bed board device, including silicon chip substrate 1, T-shaped passage 2, micro-coil I 3, micro-coil II 4, permanent magnet I 5, permanent magnet II 6, can compile
Journey AC power 7, silicon chip substrate 1 is provided with micro-coil I 3 and micro-coil II 4, and micro-coil I 3 and micro-coil II 4 are in straight line
On, and gap is left between the two；The sealing of T-shaped passage 2 is fixed in silicon chip substrate 1, and micro-coil I 3 and micro-coil II 4 are located at T
Below the horizontal channel of shape passage 2, the gap and the Vertical Channel of T-shaped passage 2 between micro-coil I 3 and micro-coil II 4 are just right；T
The top of shape passage 2 is provided with transparent cover plate, and cover plate is provided with permanent magnet I 5 or permanent magnet II 6, permanent magnet I 5 or permanent magnet
II 6 are located at the surface in the gap between micro-coil I 3 and micro-coil II 4；Micro-coil I 3, each wire of micro-coil II 4 all with
One Programmable AC Power Source 7 is connected.
It is preferred that, permanent magnet I 5 described in the utility model, the quantity of permanent magnet II 6 are one or more.
It is preferred that, the magnetic field intensity of permanent magnet II 6 described in the utility model is more than permanent magnet I 5, both intensity of magnetization sides
To vertical with the vias inner walls on its N poles opposite.
It is preferred that, micro-coil I 3 described in the utility model, micro-coil II 4 are annular, the annular diameter of micro-coil II 4
More than micro-coil I 3.
It is preferred that, the circle diameter of micro-coil II 4 described in the utility model is 10-30μM, the circle diameter of micro-coil I 3
It is 1-10μM, diameter of wire scope is 3-5μm。
It is preferred that, separation layer is provided with above micro-coil I 3 described in the utility model, micro-coil II 4.
Micro-coil I 3 described in the utility model can also be rectangular element case form, including many tops being parallel to each other lead
The base conductor 9 that line 10 and Duo Gen are parallel to each other, is provided with separation layer, base conductor 9 between top conductor line 10 and base conductor 9
In in silicon chip substrate 1, top conductor line 10 is located on separation layer, and top conductor line 10 and base conductor 9 are mutually perpendicular to, with 90 ° of phases
The sine and cosine currents of potential difference are applied on two wires of intersection to produce magnetic field gradient, micro-coil II 4 with identical frequency f
It is identical with the structure of micro-coil I 3.
It is preferred that, the line spacing of micro-coil II 4 described in the utility model is 10-30μM, the line spacing of micro-coil I 3 is 1-
10μM, diameter of wire scope is 3-5μm。
It is preferred that, separation layer is provided with above top conductor line described in the utility model.
Micro-coil I 3 described in the utility model, micro-coil II 4 pass sequentially through photoetching process, galvanoplastic manufacture in silicon chip substrate 1
Or on separation layer.
Plating cells device described in the utility model using magnetic liquid as medium, operation principle is as follows：
The magnetic liquid introduced from the porch of T-shaped passage 2（With bio-compatibility）With cell suspending liquid because inertia is made
With can be moved to channel bottom, after first placing permanent magnet I 5 on the cover plate in the middle part of passage lower end, the magnetic current in passage bottom
Body can produce the effect of a magnetic buoyancy under the externally-applied magnetic field effect that permanent magnet I 5 is produced to cell therein, and cell is in magnetic
The magnetic buoyancy being subject in fluid is directly proportional to its volume and externally-applied magnetic field gradient magnitude, the T-shaped passage 2 under magnetic field gradient effect
Middle volume is relatively large（≥10μm）The magnetic buoyancy that is subject to of cell promote it to be migrated to the left-hand component of T-shaped passage 2 compared with conference；
With remove permanent magnet I 5 place permanent magnet II 6, due to permanent magnet II 6 remanent magnetization be more than permanent magnet I 5 can produce compared with
Big magnetic field gradient, the remaining small volume in the bottom of T-shaped passage 2（<10μm）Cell under larger magnetic field gradient effect by
To magnetic buoyancy it can be promoted to be migrated to the bottom right-hand component of T-shaped passage 2, after permanent magnet II 6 to be removed, open micro-coil
3 and 4, every wire of micro-coil can carry different electric currents to have different Distribution of Magnetic Field, according to T-shaped passage 2 or so
Hecatomeral cells size, regulation programmable power supply exports the alternating current of different frequency, and when frequency is larger, micro-coil can produce big
When magnetic field gradient promotes cell movement in the weaker annulus of magnetic field intensity, now magnetic field gradient reduces reduction output frequency therewith
Cell speed can slow down and stop in annulus micro-coil, and now corresponding frequency is the critical frequency of cell, different size of
Cell moves to Circumferential coils domestic demand under magnetic field gradient and wants different size of frequency；Programmable power supply is adjusted, works as output frequency
Higher than the critical frequency of cell, the magnetic buoyancy that magnetic field gradient is produced promotes cell to be moved in passage, when output frequency is close
During the critical frequency of cell, cell speed slows down and stopped in the weaker annulus in magnetic field；As described above, being produced by micro-coil
Magnetic field gradient can realize the bed board of cell, effectively reduce the perturbed problem brought in artificial operating process to cell.
The beneficial effects of the utility model：
（1）The utility model utilizes magnetic liquid property, can be produced in the presence of externally-applied magnetic field in annular micro-coil
A raw magnetic field gradient promotes cell movement and makes cellular localization, realizes the bed board of the cell in T-shaped passage.
（2）The utility model can produce bigger magnetic field gradient to realize using micro-line coil structures compared to common coil
The motion and positioning of cell are advantageously implemented the uniform bed board of cell.
（3）The utility model is to the magnetic fluid with bio-compatibility for bed board without particular/special requirement, and it is when can be long
Between keep cytoactive.
Brief description of the drawings
Fig. 1 is structural representation of the present utility model；
Fig. 2 is the fundamental diagram of permanent magnet I；
Fig. 3 is the structural representation of the micro-coil of annular；
Fig. 4 is the magnetic chart of the micro-coil of annular；
The structural representation of the micro-coil of Fig. 5 rectangular element case forms；
Fig. 6 is the wire energization schematic diagram of the micro-coil of rectangular element case form.
In figure：1- silicon chip substrates；2-T shape passages；3- micro-coils I；4- micro-coils II；5- permanent magnets；The programmable exchanges of 7-
Power supply；8- separation layers；9- base conductors；10- top conductor lines.
Embodiment
The utility model is described in further detail with reference to the accompanying drawings and examples, but content of the present utility model is not limited to
Shown in embodiment and accompanying drawing.
Embodiment 1
A kind of plating cells device using magnetic liquid as medium, including silicon chip substrate 1（For 100 × 100mm of rectangle）、T
Shape passage 2, micro-coil I 3, micro-coil II 4, permanent magnet I 5, permanent magnet II 6, Programmable AC Power Source 7, silicon chip substrate 1 are provided with
Micro-coil I 3 and micro-coil II 4（Micro-coil I 3 and micro-coil II 4 are produced on silicon chip substrate by photoetching process and plating in the present embodiment
On 1）, micro-coil I 3 and micro-coil II 4 point-blank, and leave gap between the two；The sealing of T-shaped passage 2 is fixed on silicon
In piece substrate 1, micro-coil I 3 and micro-coil II 4 are located at below the horizontal channel of T-shaped passage 2, micro-coil I 3 and micro-coil II 4 it
Between gap and the Vertical Channel of T-shaped passage 2 it is just right；The top of T-shaped passage 2 is provided with transparent cover plate, and cover plate is provided with permanent magnet I
5 or permanent magnet II 6, the remanent magnetism of permanent magnet I 5 and permanent magnet II 6 is respectively 1 tesla and 1.2 teslas, permanent magnet I 5 or
Permanent magnet II 6 is located at the surface in the gap between micro-coil I 3 and micro-coil II 4；The circle diameter of micro-coil II 4 is 20μM,
The circle diameter of micro-coil I 3 is 10μM, micro-coil I 3, each wire of micro-coil II 4 are all connected with a programmable power supply to be come
The alternating current of different frequency size is provided, the frequency range of alternating current is 10HZ-50KHZ, as shown in Fig. 1 ~ 4.
Opening micro-coil I 3, before micro-coil II 4, first by permanent magnet I 5 be placed on the cover plate of the top of T-shaped passage 2 and
Feeder connection introduces magnetic fluid and cell suspending liquid, by larger cell separation to the left end of T-shaped passage 2, as shown in Figure 2（Micro-line
Circle 4 is omitted and is not drawn into herein）, then remove permanent magnet I 5 and the larger permanent magnet II of remanent magnetization placed in identical position
6, by less cell separation to the right-hand member of T-shaped passage 2.
Cell in the infringement of the problems such as in order to prevent electric leakage passage, micro-coil I 3 described in the present embodiment, micro-coil II 4 it is upper
Face can also set separation layer.
Embodiment 2
With reference to the structure of embodiment 1, the present embodiment is with the difference of embodiment 1, permanent magnet I 5, the quantity of permanent magnet II 6
2 pieces or polylith is respectively adopted, this magnet construction can produce the magnetic that cell is subject in the magnetic field of more high gradient, increase magnetic fluid
Property buoyancy.
Embodiment 3
With reference to the structure of embodiment 1, the present embodiment is that micro-coil I 3 described in the utility model is with the difference of embodiment 1
Rectangular element case form, including the base conductor 9 that many top conductor lines 10 being parallel to each other and Duo Gen are parallel to each other, top conductor line
Separation layer is provided between 10 and base conductor 9, base conductor 9 is located in silicon chip substrate 1, and top conductor line 10 is located on separation layer,
Top conductor line 10 and base conductor 9 are mutually perpendicular to, and sine and cosine currents with 90 ° of phase differences are applied to identical frequency f
Magnetic field gradient is produced on two wires intersected, micro-coil II 4 is identical with the structure of micro-coil I 3；Micro-coil I 3 and micro-coil
II 4 line spacing is 10 respectivelyμM and 20μM, diameter of wire is all 4μM, sine and cosine currents with 90 ° of phase differences are with phase
Same frequency f is applied on two wires of intersection to produce magnetic field gradient, as shown in Figure 5,6.
Separation layer can also be set above cell in the infringement of the problems such as in order to prevent electric leakage passage, top conductor line.
Claims (9)
1. a kind of plating cells device using magnetic liquid as medium, it is characterised in that：Silicon chip substrate（1）, T-shaped passage（2）, it is micro-
Coil I（3）, micro-coil II（4）, permanent magnet I（5）, permanent magnet II（6）, Programmable AC Power Source（7）, silicon chip substrate（1）On set
There is micro-coil I（3）With micro-coil II（4）, micro-coil I（3）With micro-coil II（4）Point-blank, and leave between the two
Gap；T-shaped passage（2）Sealing is fixed on silicon chip substrate（1）On, micro-coil I（3）With micro-coil II（4）Positioned at T-shaped passage（2）
Horizontal channel below, micro-coil I（3）With micro-coil II（4）Between gap and T-shaped passage（2）Vertical Channel it is just right；T
Shape passage（2）Top is provided with transparent cover plate, and cover plate is provided with permanent magnet I（5）Or permanent magnet II（6）, permanent magnet I（5）Or
Person's permanent magnet II（6）Positioned at micro-coil I（3）With micro-coil II（4）Between gap surface；Micro-coil I（3）, micro-coil
Ⅱ（4）Each wire all with a Programmable AC Power Source（7）Connection.
2. the plating cells device according to claim 1 using magnetic liquid as medium, it is characterised in that：Permanent magnet I
（5）, permanent magnet II（6）Quantity to be one or more.
3. the plating cells device according to claim 1 or 2 using magnetic liquid as medium, it is characterised in that：Permanent magnet
Ⅱ（6）Magnetic field intensity be more than permanent magnet I（5）, both magnetization directions are vertical with the vias inner walls on its N poles opposite.
4. the plating cells device according to claim 1 using magnetic liquid as medium, it is characterised in that：Micro-coil I
（3）, micro-coil II（4）For annular, micro-coil II（4）Annular diameter be more than micro-coil I（3）.
5. the plating cells device according to claim 4 using magnetic liquid as medium, it is characterised in that：Micro-coil II 4
Circle diameter be 10-30μM, the circle diameter of micro-coil I 3 is 1-10μM, diameter of wire scope is 3-5μm。
6. the plating cells device using magnetic liquid as medium according to claim 1,4 or 5, it is characterised in that：Micro-line
Circle I（3）, micro-coil II（4）Above be provided with separation layer.
7. the plating cells device according to claim 1 using magnetic liquid as medium, it is characterised in that：Micro-coil I（3）
For rectangular element case form, including many top conductor lines being parallel to each other（10）With many base conductors being parallel to each other（9）, top
Portion's wire（10）And base conductor（9）Between be provided with separation layer, base conductor（9）Positioned at silicon chip substrate（1）On, top conductor line
（10）On separation layer, top conductor line（10）And base conductor（9）It is mutually perpendicular to, sine and cosine with 90 ° of phase differences
Electric current is applied on two wires of intersection to produce magnetic field gradient, micro-coil II with identical frequency f（4）With micro-coil I（3）'s
Structure is identical.
8. the plating cells device according to claim 7 using magnetic liquid as medium, it is characterised in that：Micro-coil II
（4）Line spacing be 10-30μM, micro-coil I（3）Line spacing be 1-10μM, diameter of wire scope is 3-5μm。
9. the plating cells device using magnetic liquid as medium according to claim 7 or 8, it is characterised in that：Lead at top
Separation layer is provided with above line.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720054665.XU CN206512225U (en) | 2017-01-18 | 2017-01-18 | A kind of plating cells device using magnetic liquid as medium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720054665.XU CN206512225U (en) | 2017-01-18 | 2017-01-18 | A kind of plating cells device using magnetic liquid as medium |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206512225U true CN206512225U (en) | 2017-09-22 |
Family
ID=59864999
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720054665.XU Expired - Fee Related CN206512225U (en) | 2017-01-18 | 2017-01-18 | A kind of plating cells device using magnetic liquid as medium |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206512225U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106867901A (en) * | 2017-01-18 | 2017-06-20 | 昆明理工大学 | A kind of plating cells device with magnetic liquid as medium |
| CN107930547A (en) * | 2017-12-04 | 2018-04-20 | 昆明理工大学 | A kind of microreactor based on magnetic liquid hoodle |
-
2017
- 2017-01-18 CN CN201720054665.XU patent/CN206512225U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106867901A (en) * | 2017-01-18 | 2017-06-20 | 昆明理工大学 | A kind of plating cells device with magnetic liquid as medium |
| CN107930547A (en) * | 2017-12-04 | 2018-04-20 | 昆明理工大学 | A kind of microreactor based on magnetic liquid hoodle |
| CN107930547B (en) * | 2017-12-04 | 2024-03-26 | 昆明理工大学 | Microreactor based on magnetic liquid marble |
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170922 Termination date: 20200118 |