CN206512224U - A kind of plating cells device based on energization micro-coil - Google Patents
A kind of plating cells device based on energization micro-coil Download PDFInfo
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- CN206512224U CN206512224U CN201720054663.0U CN201720054663U CN206512224U CN 206512224 U CN206512224 U CN 206512224U CN 201720054663 U CN201720054663 U CN 201720054663U CN 206512224 U CN206512224 U CN 206512224U
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- Prior art keywords
- micro
- coil
- energization
- plating cells
- cell
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- 238000007747 plating Methods 0.000 title claims abstract description 18
- 229910052710 silicon Inorganic materials 0.000 claims abstract description 18
- 239000010703 silicon Substances 0.000 claims abstract description 18
- 239000000758 substrate Substances 0.000 claims abstract description 18
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 claims abstract description 17
- 238000007789 sealing Methods 0.000 claims abstract description 4
- 239000004020 conductor Substances 0.000 claims description 26
- 238000000926 separation method Methods 0.000 claims description 15
- 238000000034 method Methods 0.000 abstract description 6
- 230000033001 locomotion Effects 0.000 abstract description 3
- 230000011664 signaling Effects 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 description 35
- 239000007788 liquid Substances 0.000 description 9
- 230000009087 cell motility Effects 0.000 description 2
- 239000011553 magnetic fluid Substances 0.000 description 2
- 239000006249 magnetic particle Substances 0.000 description 2
- 238000001259 photo etching Methods 0.000 description 2
- 230000001464 adherent effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 230000030570 cellular localization Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 210000004962 mammalian cell Anatomy 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- YTCQFLFGFXZUSN-BAQGIRSFSA-N microline Chemical compound OC12OC3(C)COC2(O)C(C(/Cl)=C/C)=CC(=O)C21C3C2 YTCQFLFGFXZUSN-BAQGIRSFSA-N 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model is related to a kind of plating cells device based on energization micro-coil, belongs to biomedical engineering field.Plating cells device described in the utility model includes silicon chip substrate, passage, micro-coil, Programmable AC Power Source, and passage sealing is fixed in silicon chip substrate, is made up of a rectangular channel and a circular channel；Silicon chip substrate is provided with micro-coil, and micro-coil is located at below the circular channel of passage；Transparent cover plate is provided with above passage, each wire of micro-coil is all connected with a Programmable AC Power Source.When the power source is turned on, micro-coil can produce a magnetic field gradient, the motion and stopping for the magnetic buoyancy control cell that cell is subject to by magnetic field gradient, so signaling and can will be positioned at the predeterminated position of circular channel, so as to realize the bed board of cell by non-contacting mode.The utility model can effectively reduce the perturbed problem in artificial operating process, improve the efficiency of plating of cell.
Description
Technical field
The utility model is related to biomedical engineering field, more particularly to a kind of plating cells dress based on energization micro-coil
Put.
Background technology
The in vitro culture of cell and tissue is the important means of life science.Some cells and tissue can be in suspensions
Grown under state, but quite a few mammalian cell needs progress surface adherent.Therefore, for providing cell injuring model ring
Blake bottle, culture plate and the culture dish in border except with good transparency and it is nontoxic, sterile in addition to, also need to enable cell to paste
Wall, division and growth.But, among practical application, due to cell volume and quality very little, extraneous disturbance somewhat will be right
The motion of cell produces significant impact.And plating cells inequality can then cause the problems such as cell growth state is not good, cause reality
The failure tested.
At present, in order to reach the purpose for allowing cell to be uniformly distributed on culture vessel wall, the method generally used is to draw
" ten " word, which intersects, gently to be mixed；During inoculating cell liquid, it is aligned in the middle of culture plate and is added with pipette tips, allow cell suspension voluntarily to flow through entirely
Hole, and the volume of the cell liquid of addition need to be strictly held, it is noted that holding level with both hands and handling with care during mobile culture vessel.These behaviour
Make requirement to experimenter higher, with more subjectivity, it is desirable to have rich experience, known technical ability, slightly carelessness or
Careless manipulation will cause the failure of an experiment.Therefore, obtaining fast and convenient and reproducible method makes cell uniform in bed board
It is affixed on and improving and development for cell culture technology is may advantageously facilitate on culture vessel wall, tool is of great significance.
The soliquid that magnetic liquid is made up of nano magnetic particle, base load liquid and the part of surfactant three, be
Have the functional fluid of magnetic and mobility concurrently.Because magnetic liquid can be to non-magnetic particle therein under additional magnetic fields
A magnetic buoyancy is produced, because of this peculiar property so as to be used to realize cell separation, sieve in many scientific research fields
Choosing and cell capture etc..
The content of the invention
In order to overcome the shortcoming of prior art, the purpose of this utility model is that provide one kind is produced using energization micro-coil
The plating cells device in magnetic field, should have the advantages that cost was low, efficiency high.
In order to achieve the above object, the technical solution adopted in the utility model is：A kind of cell based on energization micro-coil
Bed board device, including silicon chip substrate 1, passage 2, micro-coil 3, Programmable AC Power Source 4, the sealing of passage 2 are fixed on silicon chip substrate 1
On, it is made up of a rectangular channel and a circular channel passage；Silicon chip substrate 1 is provided with micro-coil 3, and micro-coil 3 is located at logical
Below the circular channel in road 2；The top of passage 2 is provided with transparent cover plate, and each wire of micro-coil 3 all may be programmed with one to be exchanged
Power supply 4 is connected.
It is preferred that, micro-coil 3 described in the utility model is annular.
It is preferred that, the circle diameter of micro-coil 3 described in the utility model is 5-30μM, diameter of wire scope is 3-5μm。
It is preferred that, separation layer is provided with above micro-coil 3 described in the utility model.
It is preferred that, micro-coil 3 described in the utility model is rectangular element case form, including many tops being parallel to each other lead
The base conductor 6 that line 7 and Duo Gen are parallel to each other, is provided with separation layer 5, base conductor 6 between top conductor line 7 and base conductor 6
In in silicon chip substrate 1, top conductor line 7 is located on separation layer 5, and top conductor line 7 and base conductor 6 are mutually perpendicular to, with 90 ° of phases
The sine and cosine currents of difference are applied on two wires of intersection to produce magnetic field gradient with identical frequency f.
It is preferred that, the line spacing of micro-coil 3 described in the utility model is 5-30μM, diameter of wire scope is 3-5μm。
It is preferred that, separation layer is provided with above top conductor line described in the utility model.
Micro-coil 3 described in the utility model is by photoetching process, galvanoplastic manufacture in silicon chip substrate 1 or separation layer.
Plating cells device described in the utility model based on energization micro-coil, operation principle is as follows：
Micro-coil 3 is first opened, the magnetic liquid introduced from the porch of T-shaped passage 2（With bio-compatibility）It is outstanding with cell
Supernatant liquid can be moved due to effect of inertia to passage circular annular region, every wire of micro-coil can carry different electric currents so as to
There are different Distribution of Magnetic Field, according to the cell size in passage 2, regulation programmable power supply 4 exports the alternating current of different frequency, when
When micro-coil can produce big magnetic field gradient and promote cell movement in the weaker annulus of magnetic field intensity when frequency is larger, reduction is defeated
Going out frequency, now magnetic field gradient reduces cell speed and can slow down and stop in annulus micro-coil therewith, and now corresponding frequency is
The critical frequency of cell, different size of cell moves to Circumferential coils domestic demand under magnetic field gradient and wants different size of frequency；
Programmable power supply is adjusted, when output frequency is higher than the critical frequency of cell, the magnetic buoyancy that magnetic field gradient is produced promotes cell to exist
Moved in passage, when output frequency connects cytoproximal critical frequency, cell speed slows down and stops at the weaker annulus in magnetic field
It is interior；As described above, the magnetic field gradient produced by micro-coil can realize the bed board of cell, effectively reduce in artificial operating process
The perturbed problem brought to cell.
The beneficial effects of the utility model：
（1）The utility model utilizes magnetic liquid property, can produce one in annular micro-coil in the case of energization
Magnetic field gradient promotes cell movement and makes cellular localization, realizes the bed board of cell in passage.
（2）The utility model can produce bigger magnetic field gradient to realize using micro-line coil structures compared to common coil
The motion and positioning of cell are advantageously implemented the uniform bed board of cell.
（3）The utility model is to the magnetic fluid with bio-compatibility for bed board without particular/special requirement, and it is when can be long
Between keep cytoactive.
Brief description of the drawings
Fig. 1 is structural representation of the present utility model；
Fig. 2 is the structural representation of the micro-coil of annular；
Fig. 3 is the magnetic chart of the micro-coil of annular；
The structural representation of the micro-coil of Fig. 4 rectangular element case forms；
Fig. 5 is the wire energization schematic diagram of the micro-coil of rectangular element case form.
In figure：1- silicon chip substrates；2- passages；3- micro-coils；4- Programmable AC Power Sources；5- separation layers；6- base conductors；
7- top conductor lines.
Embodiment
The utility model is described in further detail with reference to the accompanying drawings and examples, but content of the present utility model is not limited to
Shown in embodiment and accompanying drawing.
Embodiment 1
A kind of plating cells device based on energization micro-coil, including silicon chip substrate 1（For 100 × 100mm of rectangle）, passage
2nd, micro-coil 3, Programmable AC Power Source 4, silicon chip substrate 1 are provided with micro-coil 3（In the present embodiment micro-coil 3 by photoetching process and
Plating is produced in silicon chip substrate 1）；The sealing of passage 2 is fixed in silicon chip substrate 1, and micro-coil 3 is located at the circle ring area of passage 2
Lower section, a diameter of 30mm of circular annular region；The circle diameter of micro-coil 3 is 25μM, diameter of wire is 4μM, micro-coil 3 it is every
Root wire is all connected to provide the alternating current of different frequency size, the frequency change model of alternating current with a Programmable AC Power Source
It is 10HZ-50KHZ to enclose, as shown in Fig. 1 ~ 4.Introduced in passage 2 before magnetic fluid and cell suspending liquid, first open micro-coil 3.
Cell in the infringement of the problems such as in order to prevent electric leakage passage, can also set described in the present embodiment above micro-coil 3
Put separation layer.
Embodiment 2
With reference to the structure of embodiment 1, the present embodiment is with the difference of embodiment 1, and micro-coil 3 described in the utility model is square
Shape unit case form, including the base conductor 6 that many top conductor lines 7 being parallel to each other and Duo Gen are parallel to each other, the He of top conductor line 7
Separation layer 5 is provided between base conductor 6, base conductor 6 is located in silicon chip substrate 1, and top conductor line 7 is located on separation layer, top
Wire 7 and base conductor 6 are mutually perpendicular to, and sine and cosine currents with 90 ° of phase differences are applied to intersection with identical frequency f
Magnetic field gradient is produced on two wires, the line spacing of the micro-coil 3 of rectangular element case form is 25μM, diameter of wire is 4μM,
Sine and cosine currents with 90 ° of phase differences are applied on two wires of intersection to produce magnetic field gradient with identical frequency f,
As shown in Figure 4,5.
Separation layer can also be set above cell in the infringement of the problems such as in order to prevent electric leakage passage, top conductor line.
Claims (7)
1. a kind of plating cells device based on energization micro-coil, it is characterised in that：Including silicon chip substrate（1）, passage（2）, it is micro-
Coil（3）, Programmable AC Power Source（4）, passage（2）Sealing is fixed on silicon chip substrate（1）On, by a rectangular channel and one
Circular channel is constituted；Silicon chip substrate（1）It is provided with micro-coil（3）, micro-coil（3）Positioned at passage（2）Circular channel below；It is logical
Road（2）Top is provided with transparent cover plate, micro-coil（3）Each wire all with a Programmable AC Power Source（4）Connection.
2. the plating cells device according to claim 1 based on energization micro-coil, it is characterised in that：Micro-coil（3）For
Annular.
3. the plating cells device according to claim 2 based on energization micro-coil, it is characterised in that：Micro-coil（3）'s
Circle diameter is 5-30μM, diameter of wire scope is 3-5μm。
4. the plating cells device based on energization micro-coil according to claim 1,2 or 3, it is characterised in that：Micro-coil
（3）Above be provided with separation layer.
5. the plating cells device according to claim 1 based on energization micro-coil, it is characterised in that：Micro-coil（3）For
Rectangular element case form, including many top conductor lines being parallel to each other（7）With many base conductors being parallel to each other（6）, top
Wire（7）And base conductor（6）Between be provided with separation layer（5）, base conductor (6) is positioned at silicon chip substrate（1）On, top conductor line
（7）Positioned at separation layer（5）On, top conductor line（7）And base conductor（6）It is mutually perpendicular to, sine and cosine with 90 ° of phase differences
Electric current is applied on two wires of intersection to produce magnetic field gradient with identical frequency f.
6. the plating cells device according to claim 5 based on energization micro-coil, it is characterised in that：Micro-coil（3）'s
Line spacing is 5-30μM, diameter of wire scope is 3-5μm。
7. the plating cells device based on energization micro-coil according to claim 5 or 6, it is characterised in that：Top conductor line
（7）Separation layer is provided with above.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720054663.0U CN206512224U (en) | 2017-01-18 | 2017-01-18 | A kind of plating cells device based on energization micro-coil |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720054663.0U CN206512224U (en) | 2017-01-18 | 2017-01-18 | A kind of plating cells device based on energization micro-coil |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206512224U true CN206512224U (en) | 2017-09-22 |
Family
ID=59864954
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720054663.0U Expired - Fee Related CN206512224U (en) | 2017-01-18 | 2017-01-18 | A kind of plating cells device based on energization micro-coil |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206512224U (en) |
-
2017
- 2017-01-18 CN CN201720054663.0U patent/CN206512224U/en not_active Expired - Fee Related
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| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170922 Termination date: 20200118 |