CN106867901A - A kind of plating cells device with magnetic liquid as medium - Google Patents
A kind of plating cells device with magnetic liquid as medium Download PDFInfo
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- CN106867901A CN106867901A CN201710033138.5A CN201710033138A CN106867901A CN 106867901 A CN106867901 A CN 106867901A CN 201710033138 A CN201710033138 A CN 201710033138A CN 106867901 A CN106867901 A CN 106867901A
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- magnetic liquid
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- 239000007788 liquid Substances 0.000 title claims abstract description 26
- 238000007747 plating Methods 0.000 title claims abstract description 19
- 229910052710 silicon Inorganic materials 0.000 claims abstract description 18
- 239000010703 silicon Substances 0.000 claims abstract description 18
- 239000000758 substrate Substances 0.000 claims abstract description 18
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 claims abstract description 17
- 238000007789 sealing Methods 0.000 claims abstract description 4
- 239000004020 conductor Substances 0.000 claims description 26
- 238000000926 separation method Methods 0.000 claims description 17
- 230000005415 magnetization Effects 0.000 claims description 4
- YTCQFLFGFXZUSN-BAQGIRSFSA-N microline Chemical compound OC12OC3(C)COC2(O)C(C(/Cl)=C/C)=CC(=O)C21C3C2 YTCQFLFGFXZUSN-BAQGIRSFSA-N 0.000 claims description 4
- 238000000034 method Methods 0.000 abstract description 6
- 210000004027 cell Anatomy 0.000 description 43
- 239000011553 magnetic fluid Substances 0.000 description 5
- 230000000694 effects Effects 0.000 description 4
- 230000009087 cell motility Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000033001 locomotion Effects 0.000 description 2
- 239000006249 magnetic particle Substances 0.000 description 2
- 238000001259 photo etching Methods 0.000 description 2
- 230000001464 adherent effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 230000030570 cellular localization Effects 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000005426 magnetic field effect Effects 0.000 description 1
- 230000005389 magnetism Effects 0.000 description 1
- 210000004962 mammalian cell Anatomy 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
- C12M25/06—Plates; Walls; Drawers; Multilayer plates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M45/00—Means for pre-treatment of biological substances
- C12M45/07—Means for pre-treatment of biological substances by electrical or electromagnetic forces
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Electromagnetism (AREA)
- Immunology (AREA)
- Water Treatment By Electricity Or Magnetism (AREA)
Abstract
The present invention relates to a kind of plating cells device with magnetic liquid as medium, belong to biomedical engineering field.Device of the present invention includes silicon chip substrate, T-shaped passage, micro-coil I, micro-coil II, permanent magnet I, permanent magnet II, Programmable AC Power Source, silicon chip substrate is provided with micro-coil I and micro-coil II, micro-coil I and micro-coil II point-blank, and leave gap between the two；The sealing of T-shaped passage is fixed in silicon chip substrate, and micro-coil I and micro-coil II are located at below the horizontal channel of T-shaped passage, and the gap between micro-coil I and micro-coil II is just right with the Vertical Channel of T-shaped passage；T-shaped passage top is provided with transparent cover plate, and cover plate is provided with permanent magnet I or permanent magnet II, and permanent magnet I or permanent magnet II are located at the surface in the gap between micro-coil I and micro-coil II；Micro-coil I, each wire of micro-coil II are all connected with a Programmable AC Power Source.The present invention can effectively reduce the perturbed problem in artificial operating process, improve the efficiency of plating of cell.
Description
Technical field
The present invention relates to biomedical engineering field, more particularly to a kind of plating cells dress with magnetic liquid as medium
Put.
Background technology
The in vitro culture of cell and tissue is the important means of life science.Some cells and tissue can be in suspensions
Grown under state, but quite a few mammalian cell need to carry out surface it is adherent.Therefore, for providing cell injuring model ring
The blake bottle in border, culture plate and culture dish except with good transparency and it is nontoxic, aseptic in addition to, also need to enable cell to paste
Wall, division and growth.But, in the middle of practical application, due to cell volume and quality very little, extraneous disturbance somewhat will be right
The motion of cell produces significant impact.And plating cells inequality can then cause the problems such as cell growth state is not good, cause reality
The failure tested.
At present, in order to reach the purpose for allowing cell to be uniformly distributed on culture vessel wall, the method for generally using is to draw
" ten " word intersection is gently mixed；During inoculating cell liquid, it is aligned in the middle of culture plate with pipette tips and is added, allows cell suspension voluntarily to flow through entirely
Hole, and the volume of the cell liquid of addition need to be strictly held, it is noted that holding level with both hands and handling with care during mobile culture vessel.These behaviour
Make requirement to experimenter higher, with more subjectivity, it is desirable to have rich experience, familiar technical ability, slightly carelessness or
Careless manipulation will cause the failure of an experiment.Therefore, obtaining fast and convenient and reproducible method makes cell uniform in bed board
It is affixed on culture vessel wall and may advantageously facilitate improving and development for cell culture technology, tool is of great significance.
The soliquid that magnetic liquid is made up of nano magnetic particle, base load liquid and the part of surfactant three, be
Have the functional fluid of magnetic and mobility concurrently.Because magnetic liquid can be to non-magnetic particle therein under additional magnetic fields
A magnetic buoyancy is produced, because this peculiar property in many scientific research fields so as to be used to realize cell separation, sieve
Choosing and cell capture etc..
The content of the invention
In order to overcome the shortcoming of prior art, magnetic liquid is used as medium it is an object of the invention to provide a kind of
Plating cells device, should have the advantages that low cost, efficiency high.
In order to achieve the above object, the technical solution adopted by the present invention is：A kind of cell paving with magnetic liquid as medium
Panel assembly, including silicon chip substrate 1, T-shaped passage 2, micro-coil I 3, micro-coil II 4, permanent magnet I 5, permanent magnet II 6, programmable friendship
Stream power supply 7, silicon chip substrate 1 is provided with micro-coil I 3 and micro-coil II 4, micro-coil I 3 and micro-coil II 4 point-blank, and
Gap is left between the two；The sealing of T-shaped passage 2 is fixed in silicon chip substrate 1, and micro-coil I 3 and micro-coil II 4 are located at T-shaped passage
2 horizontal channel lower section, the gap between micro-coil I 3 and micro-coil II 4 is just right with the Vertical Channel of T-shaped passage 2；T-shaped passage
2 tops are provided with transparent cover plate, and cover plate is provided with permanent magnet I 5 or permanent magnet II 6, and permanent magnet I 5 or permanent magnet II 6 are located at
The surface in the gap between micro-coil I 3 and micro-coil II 4；Micro-coil I 3, each wire of micro-coil II 4 all can with one
Programming AC power 7 is connected.
Preferably, permanent magnet of the present invention I 5, the quantity of permanent magnet II 6 are one or more.
Preferably, the magnetic field intensity of permanent magnet of the present invention II 6 is more than permanent magnet I 5, and both magnetization directions are equal
Vias inner walls with its N poles opposite are vertical.
Preferably, micro-coil of the present invention I 3, micro-coil II 4 are annular, the annular of micro-coil II 4 with diameter greater than
Micro-coil I 3.
Preferably, the circle diameter of micro-coil of the present invention II 4 is 10-30μM, the circle diameter of micro-coil I 3 is 1-
10μM, diameter of wire scope is 3-5μm。
Preferably, it is provided with separation layer above micro-coil of the present invention I 3, micro-coil II 4.
Micro-coil of the present invention I 3 can also be rectangular element case form, including many top conductor lines 10 being parallel to each other
With many base conductors 9 being parallel to each other, separation layer is provided between top conductor line 10 and base conductor 9, base conductor 9 is located at silicon
In piece substrate 1, top conductor line 10 is located on separation layer, and top conductor line 10 and base conductor 9 are mutually perpendicular to, with 90 ° of phase differences
Sinusoidal and cosine currents be applied on two wires of intersection to produce magnetic field gradient with same frequency f, micro-coil II 4 with it is micro-
The structure of coil I 3 is identical.
Preferably, the line spacing of micro-coil of the present invention II 4 is 10-30μM, the line spacing of micro-coil I 3 is 1-10μM,
Diameter of wire scope is 3-5μm。
Preferably, it is provided with separation layer above top conductor line of the present invention.
Micro-coil of the present invention I 3, micro-coil II 4 pass sequentially through photoetching process, galvanoplastic manufacture in silicon chip substrate 1 or
On separation layer.
Plating cells device with magnetic liquid as medium of the present invention, operation principle is as follows：
From the magnetic liquid that the porch of T-shaped passage 2 introduces（With bio-compatibility）With cell suspending liquid due to effect of inertia meeting
Moved to channel bottom, first placed on the cover plate in the middle part of passage lower end after permanent magnet I 5, the magnetic fluid in passage bottom exists
An effect for magnetic buoyancy can be produced to cell therein, cell is in magnetic fluid under the externally-applied magnetic field effect that permanent magnet I 5 is produced
In the magnetic buoyancy that is subject to be directly proportional to its volume and externally-applied magnetic field gradient magnitude, the body in T-shaped passage 2 under magnetic field gradient effect
Product is relatively large（≥10μm）The magnetic buoyancy that is subject to of cell promote it to be migrated to the left-hand component of T-shaped passage 2 compared with conference；With
Remove permanent magnet I 5 place permanent magnet II 6, due to the remanent magnetization of permanent magnet II 6 can be produced more than permanent magnet I 5 it is larger
Magnetic field gradient, the remaining small volume in the bottom of T-shaped passage 2（<10μm）Cell be subject under larger magnetic field gradient effect
Magnetic buoyancy can promote it to be migrated to the bottom right-hand component of T-shaped passage 2, after permanent magnet to be removed II 6, open the He of micro-coil 3
4, every wire of micro-coil can carry different electric currents so as to there is different Distribution of Magnetic Field, according to the left and right sides of T-shaped passage 2
Cell size, regulation programmable power supply exports the alternating current of different frequency, and when frequency is larger, micro-coil can produce big magnetic field
When gradient promotes cell movement in the weaker annulus of magnetic field intensity, now magnetic field gradient reduces cell to reduction output frequency therewith
Speed can slow down and stop in annulus micro-coil, and now corresponding frequency is the critical frequency of cell, different size of cell
Circumferential coils domestic demand is moved under magnetic field gradient and wants different size of frequency；Regulation programmable power supply, when output frequency is higher than
The critical frequency of cell, the magnetic buoyancy that magnetic field gradient is produced promotes cell to be moved in passage, when output frequency close to cell
Critical frequency when, cell speed slows down and stops in the weaker annulus in magnetic field；As described above, the magnetic produced by micro-coil
Field gradient can realize the bed board of cell, effectively reduce the perturbed problem brought to cell in artificial operating process.
Beneficial effects of the present invention：
（1）The present invention utilizes magnetic liquid property, can produce a magnetic in annular micro-coil in the presence of externally-applied magnetic field
Field gradient promotes cell movement and makes cellular localization, realizes the bed board of the cell in T-shaped passage.
（2）The present invention can produce bigger magnetic field gradient to realize cell using micro-line coil structures compared to common coil
Motion and positioning be advantageously implemented the uniform bed board of cell.
（3）To the magnetic fluid with bio-compatibility for bed board without particular/special requirement, and it can be protected the present invention for a long time
Hold cytoactive.
Brief description of the drawings
Fig. 1 is structural representation of the invention；
Fig. 2 is the fundamental diagram of permanent magnet I；
Fig. 3 is the structural representation of the micro-coil of annular；
Fig. 4 is the magnetic chart of the micro-coil of annular；
The structural representation of the micro-coil of Fig. 5 rectangular element case forms；
Fig. 6 is the wire energization schematic diagram of the micro-coil of rectangular element case form.
In figure：1- silicon chip substrates；2-T shape passages；3- micro-coils I；4- micro-coils II；5- permanent magnets；The programmable exchanges of 7-
Power supply；8- separation layers；9- base conductors；10- top conductor lines.
Specific embodiment
The invention will be further described with reference to the accompanying drawings and examples, but present disclosure is not limited to embodiment and accompanying drawing
It is shown.
Embodiment 1
A kind of plating cells device with magnetic liquid as medium, including silicon chip substrate 1（It is 100 × 100mm of rectangle）, T-shaped lead to
Road 2, micro-coil I 3, micro-coil II 4, permanent magnet I 5, permanent magnet II 6, Programmable AC Power Source 7, silicon chip substrate 1 are provided with micro-line
Enclose I 3 and micro-coil II 4（Micro-coil I 3 and micro-coil II 4 are produced on silicon chip substrate 1 by photoetching process and plating in the present embodiment
On）, micro-coil I 3 and micro-coil II 4 point-blank, and leave gap between the two；The sealing of T-shaped passage 2 is fixed on silicon chip
In substrate 1, micro-coil I 3 and micro-coil II 4 are located at below the horizontal channel of T-shaped passage 2, between micro-coil I 3 and micro-coil II 4
Gap it is just right with the Vertical Channel of T-shaped passage 2；The top of T-shaped passage 2 is provided with transparent cover plate, and cover plate is provided with permanent magnet I 5
Or permanent magnet II 6, the remanent magnetism of permanent magnet I 5 and permanent magnet II 6 is respectively 1 tesla and 1.2 teslas, permanent magnet I 5 or
Permanent magnet II 6 is located at the surface in the gap between micro-coil I 3 and micro-coil II 4；The circle diameter of micro-coil II 4 is 20μM,
The circle diameter of micro-coil I 3 is 10μM, micro-coil I 3, each wire of micro-coil II 4 are all connected with a programmable power supply and
The alternating current of different frequency size is provided, the frequency range of alternating current is 10HZ-50KHZ, as shown in Fig. 1 ~ 4.
Opening micro-coil I 3, before micro-coil II 4, first by permanent magnet I 5 be placed on the cover plate of the top of T-shaped passage 2 and
Feeder connection introduces magnetic fluid and cell suspending liquid, by larger cell separation to the left end of T-shaped passage 2, as shown in Figure 2（Micro-line
Circle 4 is omitted and is not drawn into herein）, then remove permanent magnet I 5 and place the larger permanent magnet II of remanent magnetization in identical position
6, by less cell separation to the right-hand member of T-shaped passage 2.
Cell in the infringement of the problems such as in order to prevent electric leakage passage, micro-coil I 3 described in the present embodiment, micro-coil II 4 it is upper
Face can also set separation layer.
Embodiment 2
With reference to the structure of embodiment 1, the present embodiment is that permanent magnet I 5, the quantity of permanent magnet II 6 are distinguished with the difference of embodiment 1
Using 2 pieces or polylith, this magnet construction can produce the magnetic field of more high gradient, and the magnetic that cell is subject in increase magnetic fluid is floated
Power.
Embodiment 3
With reference to the structure of embodiment 1, the present embodiment is that micro-coil of the present invention I 3 is rectangular element with the difference of embodiment 1
Case form, including the base conductor 9 that many top conductor lines 10 being parallel to each other and Duo Gen are parallel to each other, top conductor line 10 and bottom
Separation layer is provided between wire 9, base conductor 9 is located in silicon chip substrate 1, and top conductor line 10 is located on separation layer, top conductor line
10 and base conductor 9 be mutually perpendicular to, the sinusoidal and cosine currents with 90 ° of phase differences are applied to the two of intersection with same frequency f
Magnetic field gradient is produced on root wire, micro-coil II 4 is identical with the structure of micro-coil I 3；The line of micro-coil I 3 and micro-coil II 4
Spacing is respectively 10μM and 20μM, diameter of wire is all 4μM, with 90 ° of sinusoidal and cosine currents of phase difference with same frequency f
It is applied on two wires of intersection to produce magnetic field gradient, as shown in Figure 5,6.
Cell in the infringement of the problems such as in order to prevent electric leakage passage, can also set separation layer above top conductor line.
Claims (9)
1. a kind of plating cells device with magnetic liquid as medium, it is characterised in that：Silicon chip substrate（1）, T-shaped passage（2）, it is micro-
Coil I（3）, micro-coil II（4）, permanent magnet I（5）, permanent magnet II（6）, Programmable AC Power Source（7）, silicon chip substrate（1）On set
There is micro-coil I（3）With micro-coil II（4）, micro-coil I（3）With micro-coil II（4）Point-blank, and leave between the two
Gap；T-shaped passage（2）Sealing is fixed on silicon chip substrate（1）On, micro-coil I（3）With micro-coil II（4）Positioned at T-shaped passage（2）
Horizontal channel lower section, micro-coil I（3）With micro-coil II（4）Between gap and T-shaped passage（2）Vertical Channel it is just right；T
Shape passage（2）Top is provided with transparent cover plate, and cover plate is provided with permanent magnet I（5）Or permanent magnet II（6）, permanent magnet I（5）Or
Person's permanent magnet II（6）Positioned at micro-coil I（3）With micro-coil II（4）Between gap surface；Micro-coil I（3）, micro-coil
Ⅱ（4）Each wire all with a Programmable AC Power Source（7）Connection.
2. the plating cells device with magnetic liquid as medium according to claim 1, it is characterised in that：Permanent magnet I
（5）, permanent magnet II（6）Quantity be one or more.
3. the plating cells device with magnetic liquid as medium according to claim 1 and 2, it is characterised in that：Permanent magnet
Ⅱ（6）Magnetic field intensity be more than permanent magnet I（5）, vias inner walls of both magnetization directions with its N poles opposite are vertical.
4. the plating cells device with magnetic liquid as medium according to claim 1, it is characterised in that：Micro-coil I
（3）, micro-coil II（4）It is annular, micro-coil II（4）Annular with diameter greater than micro-coil I（3）.
5. the plating cells device with magnetic liquid as medium according to claim 4, it is characterised in that：Micro-coil II 4
Circle diameter be 10-30μM, the circle diameter of micro-coil I 3 is 1-10μM, diameter of wire scope is 3-5μm。
6. the plating cells device with magnetic liquid as medium according to claim 1,4 or 5, it is characterised in that：Micro-line
Circle I（3）, micro-coil II（4）Above be provided with separation layer.
7. the plating cells device with magnetic liquid as medium according to claim 1, it is characterised in that：Micro-coil I（3）
It is rectangular element case form, including many top conductor lines being parallel to each other（10）With many base conductors being parallel to each other（9）, top
Portion's wire（10）And base conductor（9）Between be provided with separation layer, base conductor（9）Positioned at silicon chip substrate（1）On, top conductor line
（10）On separation layer, top conductor line（10）And base conductor（9）It is mutually perpendicular to, with 90 ° of sines and cosine of phase difference
Electric current is applied on two wires of intersection to produce magnetic field gradient, micro-coil II with same frequency f（4）With micro-coil I（3）'s
Structure is identical.
8. the plating cells device with magnetic liquid as medium according to claim 7, it is characterised in that：Micro-coil II
（4）Line spacing be 10-30μM, micro-coil I（3）Line spacing be 1-10μM, diameter of wire scope is 3-5μm。
9. the plating cells device with magnetic liquid as medium according to claim 7 or 8, it is characterised in that：Lead at top
Separation layer is provided with above line.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710033138.5A CN106867901A (en) | 2017-01-18 | 2017-01-18 | A kind of plating cells device with magnetic liquid as medium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710033138.5A CN106867901A (en) | 2017-01-18 | 2017-01-18 | A kind of plating cells device with magnetic liquid as medium |
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| CN106867901A true CN106867901A (en) | 2017-06-20 |
Family
ID=59158631
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| Application Number | Title | Priority Date | Filing Date |
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| CN201710033138.5A Pending CN106867901A (en) | 2017-01-18 | 2017-01-18 | A kind of plating cells device with magnetic liquid as medium |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108745430A (en) * | 2018-07-11 | 2018-11-06 | 昆明理工大学 | It is a kind of using magnetic droplet as the device of medium directed transport drop and directed transport method |
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Application publication date: 20170620 |