CN206396252U - One kind is applied to single celled Tissue Culture Dish - Google Patents

One kind is applied to single celled Tissue Culture Dish Download PDF

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Publication number
CN206396252U
CN206396252U CN201621437321.9U CN201621437321U CN206396252U CN 206396252 U CN206396252 U CN 206396252U CN 201621437321 U CN201621437321 U CN 201621437321U CN 206396252 U CN206396252 U CN 206396252U
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CN
China
Prior art keywords
cylinder
circular groove
lid
cell
culture
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN201621437321.9U
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Chinese (zh)
Inventor
牟丽莎
蔡志明
张青
周明
陈继承
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Shenzhen Second Peoples Hospital
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Shenzhen Second Peoples Hospital
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Priority to CN201621437321.9U priority Critical patent/CN206396252U/en
Application granted granted Critical
Publication of CN206396252U publication Critical patent/CN206396252U/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

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Abstract

The utility model is related to a kind of cell culture ware, in particular it is that one kind is applied to single celled Tissue Culture Dish, including culture liquid bath, lid, cylinder I, peg, cylinder II, screen pack and circular groove, a variety of different cells or the single cell of multi-quantity can not only be cultivated under the same conditions in nutrient solution, and the culture of single or various kinds of cell can be completed under different conditions in nutrient solution.Culture liquid bath is threadedly attached in lid lower end, nutrient solution trench bottom is provided with four circular grooves, cover provided with four manholes, the manhole covered is coaxial with circular groove, covers end and is connected with cylinder II by way of hot melt, and the mouth of cylinder II and the manhole that covers are interconnected, cylinder II on circular groove inwall with being equipped with screw thread, cylinder I coordinates with cylinder II and circular groove screw thread, and peg hangs over the upper end of cylinder I, and peg lower end is by being welded to connect screen pack.

Description

One kind is applied to single celled Tissue Culture Dish
Technical field
The utility model is related to a kind of cell culture ware, is in particular that one kind is applied to single celled cell culture Ware.
Background technology
Cell culture technology is also cell clone technology, and the regular noun in biology is cell culture technology.No matter For whole biotechnology, or for the biological cloning technology of one of them, cell culture is all one essential Process, the extensive clone of cell culture inherently cell.Cell culture, the both culture including microbial cell, cell Culture technique can be passed through mass propgation by a cell turns into many cells that are simple unicellular or seldom breaking up, and this is clone The essential link of technology, and the clone of cell culture inherently cell.Substantial amounts of cell is obtained by cell culture Or its metabolite.Because biological product is got from cell, it is possible to say cell culture technology be in biotechnology most Core, most basic technology.Cell culture work has been widely used in the every field such as biology, medical science, new drug development, into For one of most important basic science.Culture dish is the instrument commonly used in cell cultivation process, but present culture dish is mostly Single type cell can only be individually cultivated, it is if desired then very inconvenient during paired observation.
The content of the invention
The utility model is applied to single celled Tissue Culture Dish mainly solving the technical problems that providing one kind, not only may be used To cultivate a variety of different cells or the single cell of multi-quantity under the same conditions in nutrient solution, and can be in nutrient solution not The culture of single or various kinds of cell is completed with conditions of.
In order to solve the above technical problems, the utility model is related to a kind of cell culture ware, in particular it is a kind of suitable For single celled Tissue Culture Dish, including culture liquid bath, lid, cylinder I, peg, cylinder II, screen pack and circular groove, not only may be used To cultivate a variety of different cells or the single cell of multi-quantity under the same conditions in nutrient solution, and can be in nutrient solution not The culture of single or various kinds of cell is completed with conditions of.
Culture liquid bath is threadedly attached in lid lower end, and nutrient solution trench bottom is covered provided with four circles provided with four circular grooves Shape through hole, the manhole covered is coaxial with circular groove, covers end and is connected with cylinder II by way of hot melt, and cylinder II Mouth is interconnected with the manhole covered, and cylinder II is with being equipped with screw thread, cylinder I and cylinder II and circular groove spiral shell on circular groove inwall Line coordinates, and peg hangs over the upper end of cylinder I, and peg lower end is by being welded to connect screen pack.
As the further optimization of the technical program, the utility model one kind is applied to described in single celled Tissue Culture Dish Screen pack on filter opening diameter be less than be cultured cell minimum diameter.
As the further optimization of the technical program, the utility model one kind is applied to described in single celled Tissue Culture Dish Lid on manhole, the hole on cylinder II it is identical with the diameter of circular groove.
As the further optimization of the technical program, the utility model one kind is applied to described in single celled Tissue Culture Dish Lid, cylinder I and cylinder II be transparent.
As the further optimization of the technical program, the utility model one kind is applied to described in single celled Tissue Culture Dish Screen pack it is identical with the diameter in the hole on cylinder I.
A kind of having the beneficial effect that suitable for single celled Tissue Culture Dish of the utility model:
The utility model one kind is applied to single celled Tissue Culture Dish, not only can under the same conditions be trained in nutrient solution A variety of different cells or the single cell of multi-quantity are supported, and single or a variety of can be completed under different conditions in nutrient solution The culture of cell.
Brief description of the drawings
The utility model is described in more detail with specific implementation method below in conjunction with the accompanying drawings.
Fig. 1 is a kind of cross section structure diagram suitable for single celled Tissue Culture Dish of the utility model.
Fig. 2 is a kind of dimensional structure diagram suitable for single celled Tissue Culture Dish of the utility model.
In figure:Cultivate liquid bath 1;Lid 2;Cylinder I 3;Peg 4;Cylinder II 5;Screen pack 6;Circular groove 7.
Embodiment
Embodiment one:
With reference to Fig. 1,2 explanation present embodiments, the utility model is related to a kind of cell culture ware, in particular It is a kind of suitable for single celled Tissue Culture Dish, including cultivates liquid bath 1, lid 2, cylinder I 3, peg 4, cylinder II 5, screen pack 6 With circular groove 7, a variety of different cells or the single cell of multi-quantity can be not only cultivated under the same conditions in nutrient solution, and The culture of single or various kinds of cell can be completed under different conditions in nutrient solution.
Culture liquid bath 1 is threadedly attached in the lower end of lid 2, and culture liquid bath 1 bottom is provided with four provided with four circular grooves 7, lid 2 Manhole and circular groove 7 on individual manhole, lid 2 is coaxial, and the upper end of lid 2 is connected with cylinder II 5 by way of hot melt, and The mouth of cylinder II 5 is interconnected with the manhole on lid 2, cylinder II 5 with being equipped with screw thread on the inwall of circular groove 7, cylinder I 3 with Cylinder II 5 and the screw thread of circular groove 7 coordinate, and peg 4 hangs over the upper end of cylinder I 3, and the lower end of peg 4 is by being welded to connect screen pack 6.Make During with identical nutrient solution culture single or various kinds of cell, lid 2 is first screwed in culture liquid bath 1 upper end, cylinder I 3 is then passed through into spiral shell Line moves to the lower end of lid 2, and cylinder I 3 is not contacted with culture liquid bath 1 bottom surface, nutrient solution is subsequently poured into, to the liquid level of nutrient solution More than screen pack 6, then the upper end of screen pack 6 that cell is poured into four cylinders I 3 respectively will be cultured, then proceedes to other cultures Operation.During using different nutrient solution culture single or various kinds of cell, cylinder I 3 is moved downwardly to and is connected with circular groove 7, is made Cylinder I 3 and one confined space of formation of circular groove 7, then pour into different types of nutrient solution the cylinder being connected with circular groove 7 respectively I 3 and with the cylinder I 3 that connect of culture liquid bath 1 is interior is cultivated.
Embodiment two:
With reference to Fig. 1,2 explanation present embodiments, present embodiment is described further to embodiment one, described Filter opening diameter on screen pack 6, which is less than, is cultured cell minimum diameter.
Embodiment three:
With reference to Fig. 1,2 explanation present embodiments, present embodiment is described further to embodiment one, described The hole on manhole, cylinder II 5 on lid 2 is identical with the diameter of circular groove 7.
Embodiment four:
With reference to Fig. 1,2 explanation present embodiments, present embodiment is described further to embodiment one, described Lid 2, cylinder I 3 and cylinder II 5 are transparent.
Embodiment five:
With reference to Fig. 1,2 explanation present embodiments, present embodiment is described further to embodiment one, described Screen pack 6 is identical with the diameter in the hole on cylinder I 3.
Operation principle of the present utility model is:During using identical nutrient solution culture single or various kinds of cell, first lid 2 is screwed in The upper end of liquid bath 1 is cultivated, does not then connect cylinder I 3 with culture liquid bath 1 bottom surface by threading action to the lower end of lid 2, and cylinder I 3 Touch, be subsequently poured into nutrient solution, the liquid level to nutrient solution exceedes screen pack 6, then cell will be cultured and pour into four cylinders I 3 respectively The interior upper end of screen pack 6, then proceedes to other culture operations.During using different nutrient solution culture single or various kinds of cell, Cylinder I 3 is moved downwardly to and is connected with circular groove 7, makes cylinder I 3 and one confined space of formation of circular groove 7, then by variety classes Nutrient solution pour into the cylinder I 3 that is connected with circular groove 7 respectively and the cylinder I 3 that connect with culture liquid bath 1 is interior is cultivated.
Certainly, described above is not limitation of the utility model, and the utility model is also not limited to the example above, this skill The variations, modifications, additions or substitutions that the those of ordinary skill in art field is made in essential scope of the present utility model, also belong to In protection domain of the present utility model.

Claims (5)

1. one kind is applied to single celled Tissue Culture Dish, including culture liquid bath (1), lid (2), cylinder I (3), peg (4), circle II (5) of cylinder, screen pack (6) and circular groove (7), it is characterised in that:Culture liquid bath (1) is threadedly attached in lid (2) lower end, cultivates Liquid bath (1) bottom is provided with four circular grooves (7), and lid (2) is provided with four manholes, the manhole covered on (2) and circular groove (7) Coaxially, lid (2) upper end is connected with cylinder II (5), and the mouth and the circle on lid (2) of cylinder II (5) by way of hot melt Through hole is interconnected, and cylinder II (5) is with being equipped with screw thread, cylinder I (3) and cylinder II (5) and circular groove (7) on circular groove (7) inwall Screw thread coordinates, and peg (4) hangs over cylinder I (3) upper end, and peg (4) lower end is by being welded to connect screen pack (6).
2. it is according to claim 1 a kind of suitable for single celled Tissue Culture Dish, it is characterised in that:Described screen pack (6) the filter opening diameter on, which is less than, is cultured cell minimum diameter.
3. it is according to claim 1 a kind of suitable for single celled Tissue Culture Dish, it is characterised in that:Described lid (2) On manhole, the hole on cylinder II (5) it is identical with the diameter of circular groove (7).
4. it is according to claim 1 a kind of suitable for single celled Tissue Culture Dish, it is characterised in that:Described lid (2), Cylinder I (3) and cylinder II (5) are transparent.
5. it is according to claim 1 a kind of suitable for single celled Tissue Culture Dish, it is characterised in that:Described screen pack (6) it is identical with the diameter in the hole on cylinder I (3).
CN201621437321.9U 2016-12-26 2016-12-26 One kind is applied to single celled Tissue Culture Dish Expired - Fee Related CN206396252U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201621437321.9U CN206396252U (en) 2016-12-26 2016-12-26 One kind is applied to single celled Tissue Culture Dish

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201621437321.9U CN206396252U (en) 2016-12-26 2016-12-26 One kind is applied to single celled Tissue Culture Dish

Publications (1)

Publication Number Publication Date
CN206396252U true CN206396252U (en) 2017-08-11

Family

ID=59511611

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201621437321.9U Expired - Fee Related CN206396252U (en) 2016-12-26 2016-12-26 One kind is applied to single celled Tissue Culture Dish

Country Status (1)

Country Link
CN (1) CN206396252U (en)

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GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20170811

Termination date: 20171226

CF01 Termination of patent right due to non-payment of annual fee