CN206089661U - Biological nanometer paramagnetic particle method extracts serum virus RNA kit - Google Patents
Biological nanometer paramagnetic particle method extracts serum virus RNA kit Download PDFInfo
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- CN206089661U CN206089661U CN201621121683.7U CN201621121683U CN206089661U CN 206089661 U CN206089661 U CN 206089661U CN 201621121683 U CN201621121683 U CN 201621121683U CN 206089661 U CN206089661 U CN 206089661U
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Abstract
The utility model provides a biological nanometer paramagnetic particle method extracts serum virus RNA kit, includes a box body and box cover, separate for the reagent chamber in the box body and hold the chamber, the reagent intracavity is equipped with tool holder and reagent storage box, place the first reagent bottle that holds lyophilized biological nanometer magnetic bead reagent in the reagent storage box respectively, hold second reagent bottle, the third reagent bottle that holds serum schizolysis buffer solution, the fourth reagent bottle that holds RNA nucleic acid extraction reinforcing reagent, the 5th reagent bottle that holds washing buffer solution I that freeze dry reagent and dilute the buffer solution, hold washing buffer solution II's the 6th reagent bottle and hold dnaseRnase the 7th reagent bottle of free elution buffer solution. This kit structure is simple, and it is more convenient to use, and can be more convenient take, put the reagent bottle effectively improves serum virus RNA's extraction efficiency, provides the adapt circumstance for depositing of reagent simultaneously, effectively portable or deposit reagent.
Description
Technical field
The utility model belongs to biological technical field, and more particularly to a kind of magnetic bionanoparticles method extracts serum-virus RNA
Kit.
Background technology
Magnetic separation technique based on micro-nano magnetic bead life science and has faced since the birth eighties in last century
More and more applied in the fields such as bed detection.Beads enrichment technical operation is simple, and strong adaptability was both manually operable,
Can operate suitable for automation equipment very well.Magnetic bionanoparticles be one by Microbe synthesis magnetic nanoparticle, be natural
Biomagnetism nano material, with the incomparable advantage of some artificial synthesized magnetic beads.Using technique for gene engineering or knot
Chemical modification technology is closed, special biologically functional molecule can be shown in magnetic bionanoparticles surface orientation, or be coated with other materials
Such as silylation material so that magnetic bionanoparticles can be used in purifying and the extraction of common nucleic acid and specific nucleic acid molecule.
Paramagnetic particle method extracts sample of nucleic acid and has important using value in life science and field of medical examination, but is directed to
Its RNA composition of the sample extraction of separate sources is more complicated than extracting DNA, because the stability of RNA is not so good as DNA, needs to adopt
With special processing method, still need to be developed maturation, standard, easy to operate kit.
Utility model content
In order to solve above-mentioned technical problem, the utility model provides a kind of magnetic bionanoparticles method and extracts serum-virus
RNA kits, the kit is simple to operate, is specifically designed for the sample of particular source and carries out RNA extractions, can be supplied to different demands
The field such as scientific research or inspection and quarantine uses.
The utility model concrete technical scheme is as follows：
The utility model provide a kind of magnetic bionanoparticles method extract serum-virus RNA kits, including box body and with
Reagent chamber and accommodating chamber are divided into by transverse slat in the lid that the box body side is hinged, the box body, are set in the accommodating chamber
There is the pumping case being slidably connected with the box body, magnetic means is provided with the pumping case, the reagent chamber inner top both sides symmetrically set
There is fixed plate, instrument cases and reagent the storage box, the side roof part connection suspension plate of the instrument cases two, the extension are provided with the reagent chamber
Plate is overlapped on the fixed plate edge, and the reagent the storage box is located in the instrument cases bottom, the reagent the storage box respectively
It is placed with for holding the first reagent bottle of lyophilized magnetic bionanoparticles reagent, for holding freeze-dried reagent dilution buffer
Second reagent bottle, the 3rd reagent bottle for holding serum lysis buffer, for hold RNA nucleic acid extraction Contrast agents
Four reagent bottles, the 5th reagent bottle for holding lavation buffer solution I, the 6th reagent bottle for holding lavation buffer solution II and
For holding the 7th reagent bottle of Dnase/Rnase-Free elution buffers.
Further, the elevating mechanism for lifting the reagent the storage box, the lifting are additionally provided with the reagent chamber
Mechanism include be symmetricly set on the lifting assembly of both sides in the reagent chamber, be horizontally installed on the reagent chamber bottom base plate and
The gripper shoe being horizontally installed between two lifting assemblies, the reagent the storage box is placed in the gripper shoe；Wherein,
The lifting assembly includes leading screw, optical axis, dop and motor, and the leading screw top is by bearing and the fixed plate
It is pivotally connected, the leading screw bottom sequentially passes through the dop, the base plate and is connected with the output shaft of the motor, the dop
It is connected with the threads of lead screw, the optical axis top is fixed in the fixed plate, the optical axis bottom is solid through the dop
It is scheduled on the base plate；
The two ends of the gripper shoe are separately fixed on the dop of two lifting assemblies.
Further, longitudinally it is provided with the dop and connects described for wearing the first installing hole for connecing the leading screw and for wearing
Second installing hole of optical axis, is provided with the screw thread being adapted to the leading screw in first installing hole, the optical axis is through described the
Two installing holes simultaneously are used to limit the dop along the leading screw axially-movable.
Further, the side on the dop near the gripper shoe is laterally opened up for the peace of gripper shoe described in clamping
Tankage.
Preferably, the end of the gripper shoe is connected in the mounting groove, and the mounting groove top sidewall is worn and connects use
In the bolt of the fixation gripper shoe.
Preferably, some ice bags are placed with positioned at the bottom base plate in the accommodating chamber, are uniformly provided with the base plate
Through hole.
The beneficial effects of the utility model are as follows：The reagent the storage box simple structure that the utility model is provided, using more
It is convenient, can eaily take, put reagent bottle, the extraction efficiency of serum-virus RNA is effectively improved, while for the storage of reagent
There is provided suitable environment, reagent is effectively easy to carry or deposits, it is practical.
Description of the drawings
Fig. 1 is that a kind of stereochemical structure of the magnetic bionanoparticles method extraction serum-virus RNA kits described in embodiment 1 is shown
It is intended to；
Fig. 2 is the sectional view that a kind of magnetic bionanoparticles method described in embodiment 1 extracts serum-virus RNA kits；
Fig. 3 is the sectional view that a kind of magnetic bionanoparticles method described in embodiment 2 extracts serum-virus RNA kits；
Fig. 4 is the solid that a kind of magnetic bionanoparticles method described in embodiment 2 extracts dop in serum-virus RNA kits
Structural representation.
Wherein：1st, box body；2nd, lid；3rd, reagent chamber；4th, accommodating chamber；5th, pumping case；6th, fixed plate；7th, instrument cases；8th, reagent
The storage box；9th, link plate；10th, the first reagent bottle；11st, the second reagent bottle；12nd, the 3rd reagent bottle；13rd, the 4th reagent bottle；14th, the 5th
Reagent bottle；15th, the 6th reagent bottle；16th, the 7th reagent bottle；17th, base plate；18th, gripper shoe；19th, leading screw；20th, optical axis；21st, dop；
22nd, motor；23rd, the first installing hole；24th, the second installing hole；25th, mounting groove；26th, bolt；27th, ice bag；28th, magnetic means.
Specific embodiment
Below in conjunction with the accompanying drawings the utility model is described in further detail with following examples.
Embodiment 1
As shown in the figures 1 and 2, the utility model embodiment 1 provides a kind of magnetic bionanoparticles method and extracts serum-virus RNA
Kit, including box body 1 and the lid 2 that is hinged with the side of the box body 1, reagent chamber 3 is divided in the box body 1 by transverse slat
Magnetic means is provided with being provided with accommodating chamber 4, the accommodating chamber 4 in the pumping case 5 being slidably connected with the box body 1, the pumping case 5
28, magnetic means 28 can be magnet or magnetisable material, and it can be used for adsorbing magnetic bead, effectively improve carrying for serum-virus RNA
Take efficiency.
The inner top of the reagent chamber 3 is symmetrically arranged on two with fixed plate 6, is provided with instrument cases 7 in the reagent chamber 3 and reagent is deposited
Storage box 8,7 liang of side roof part connection suspension plates 9 of the instrument cases, the link plate 9 is overlapped on the edge of the fixed plate 6, and the reagent is deposited
Storage box 8 is located at the bottom of the instrument cases 7, and instrument cases 7 are overlapped in the fixed plate 6, can conveniently take out, and takes out instrument cases 7
Afterwards, reagent bottle can conveniently be taken out.
It is placed with the first reagent bottle for holding lyophilized magnetic bionanoparticles reagent in the reagent the storage box 8 respectively
10th, for holding the second reagent bottle 11 of freeze-dried reagent dilution buffer, the 3rd reagent for holding serum lysis buffer
Bottle 12, the 4th reagent bottle 13 for holding RNA nucleic acid extraction Contrast agents, the 5th reagent for holding lavation buffer solution I
Bottle 14, the 6th reagent bottle 15 for holding lavation buffer solution II and for holding Dnase/Rnase-Free elution buffers
The 7th reagent bottle 16.
Embodiment 2
The utility model embodiment 2 further provides reagent the storage box 8 on the basis of embodiment 1 can be by lifting
Mechanism moves up and down, so as to reagent bottle of conveniently taking.
As shown in figure 3, being additionally provided with the elevating mechanism for lifting the reagent the storage box 8, the liter in the reagent chamber 3
Descending mechanism includes being symmetricly set on the lifting assembly of both sides in the reagent chamber 3, being horizontally installed on the bottom of the bottom of the reagent chamber 3
Plate 17 and the gripper shoe 18 being horizontally installed between two lifting assemblies, the reagent the storage box 8 is placed on the support
On plate 18；Symmetrically arranged lifting assembly can hold up gripper shoe 18, because reagent the storage box 8 is placed in gripper shoe 18,
For this purpose, reagent the storage box 8 can will be raised by lifting assembly or be put down, more convenient reagent bottle of taking.
As shown in figure 3, it is further, define that the lifting assembly includes leading screw 19, optical axis 20, card in the technical program
21 and motor 22, the top of the leading screw 19 is pivotally connected by bearing with the fixed plate 6, and the bottom of the leading screw 19 is worn successively
Cross the dop 21, the base plate 17 to be connected with the output shaft of the motor 22, the dop 21 connects with the screw thread of the leading screw 19
Connect, the top of the optical axis 20 is fixed in the fixed plate 6, the bottom of the optical axis 20 is fixed on the bottom through the dop 21
On plate 17.
Leading screw 19 is threadedly coupled with the dop 21, and when rotating under drive of the leading screw 19 in motor 22, dop 21 is with silk
Thick stick 19 is rotated, but because optical axis 20 carries out the spacing of circumferencial direction to dop 21, so dop 21 is only capable of along leading screw about 19
Motion, and then realize lifting.
The two ends of the gripper shoe 18 are separately fixed on the dop 21 of two lifting assemblies.
As shown in figure 4, further defining the structure of dop 21 in the technical program, use is longitudinally provided with the dop 21
In wearing the first installing hole 23 for connecing the leading screw 19 and for wearing the second installing hole 24 for connecing the optical axis 20, described first installs
The screw thread being adapted to the leading screw 19 is provided with hole 23, the optical axis 20 is through second installing hole 24 and described for limiting
Dop 21 is along the axially-movable of the leading screw 19.Leading screw 19 is through the first installing hole 23 and is threadedly coupled with the first installing hole 23, light
Axle 20 passes through the second installing hole 24, when leading screw 19 is rotated, because optical axis 20 limits the circular motion of dop 21, so dop 21
Can only move up and down along optical axis 20 and leading screw 19, it is achieved thereby that lifting and falling reagent the storage box 8, effectively conveniently take, put
Reagent bottle, improves test efficiency.
As shown in figure 4, further, the side on the dop 21 near the gripper shoe 18 is laterally opened up for clamping
The mounting groove 25 of the gripper shoe 18.Mounting groove 25 can be used for the two ends of clamping gripper shoe 18, for this purpose, when dop 21 is along optical axis
20 and leading screw 19 move up and down when, dop 21 drive gripper shoe 18 move up and down, so as to realize the reagent in gripper shoe 18 store
Box 8 moves up and down, convenient to take out reagent bottle.
As shown in figure 4, the stability in order to improve gripper shoe 18, defines the end of the gripper shoe 18 in the technical program
Portion is connected in the mounting groove 25, and the top sidewall of the mounting groove 25 wears the bolt connect for fixing the gripper shoe 18
26.The two ends of gripper shoe 18 are connected in the mounting groove 25, and the bolt 26 by the top of mounting groove 25 tightens fixation, effectively
Improve the stability after gripper shoe 18 is installed.
In order to provide suitable Conservation environment for reagent, define in the technical program and be located in the accommodating chamber 4
The bottom of the base plate 17 is placed with some ice bags 27, and on the base plate 17 through hole is uniformly provided with.Cold air in ice bag 27 is by logical
Hole distributes into reagent chamber 3, and the effectively preservation for reagent provides suitable environment.
The utility model is not limited to above-mentioned preferred forms, and anyone can draw under enlightenment of the present utility model
Other various forms of products, however, make any change in its shape or structure, it is every with same as the present application or phase
Approximate technical scheme, all falls within protection domain of the present utility model.
Claims (6)
1. a kind of magnetic bionanoparticles method extracts serum-virus RNA kits, including box body (1) and with the box body (1) side
The lid (2) being hinged, it is characterised in that reagent chamber (3) and accommodating chamber (4) are divided into by transverse slat in the box body (1), it is described
It is provided with accommodating chamber (4) in the pumping case (5) being slidably connected with the box body (1), the pumping case (5) and is provided with magnetic means (28),
Reagent chamber (3) inner top is symmetrically arranged on two with fixed plate (6), is provided with instrument cases (7) in the reagent chamber (3) and reagent is deposited
Storage box (8), side roof part connection suspension plate (9) of the instrument cases (7) two, the link plate (9) is overlapped on the fixed plate (6) edge,
The reagent the storage box (8) is placed with respectively for holding in the instrument cases (7) bottom, the reagent the storage box (8)
First reagent bottle (10) of lyophilized magnetic bionanoparticles reagent, the second reagent bottle for holding freeze-dried reagent dilution buffer
(11), for hold serum lysis buffer the 3rd reagent bottle (12), for hold RNA nucleic acid extraction Contrast agents the 4th
Reagent bottle (13), the 5th reagent bottle (14) for holding lavation buffer solution I, the 6th reagent for holding lavation buffer solution II
Bottle (15) and the 7th reagent bottle (16) for holding Dnase/Rnase-Free elution buffers.
2. magnetic bionanoparticles method as claimed in claim 1 extracts serum-virus RNA kits, it is characterised in that the examination
The elevating mechanism for lifting the reagent the storage box (8) is additionally provided with agent chamber (3), the elevating mechanism includes being symmetricly set on
The lifting assembly of the interior both sides of the reagent chamber (3), the base plate (17) and horizontally set that are horizontally installed on the reagent chamber (3) bottom
Gripper shoe (18) between two lifting assemblies, the reagent the storage box (8) is placed in the gripper shoe (18)；Its
In,
The lifting assembly includes leading screw (19), optical axis (20), dop (21) and motor (22), and leading screw (19) top passes through
Bearing is pivotally connected with the fixed plate (6), and leading screw (19) bottom sequentially passes through the dop (21), the base plate (17)
It is connected with the output shaft of the motor (22), the dop (21) is threadedly coupled with the leading screw (19), optical axis (20) top
End is fixed in the fixed plate (6), and optical axis (20) bottom is fixed on the base plate (17) through the dop (21)；
The two ends of the gripper shoe (18) are separately fixed on the dop (21) of two lifting assemblies.
3. magnetic bionanoparticles method as claimed in claim 2 extracts serum-virus RNA kits, it is characterised in that the card
Longitudinally it is provided with head (21) and connects the optical axis (20) for wearing the first installing hole (23) for connecing the leading screw (19) and for wearing
The screw thread being adapted to the leading screw (19), the optical axis (20) are provided with second installing hole (24), first installing hole (23)
Through second installing hole (24) and for limiting the dop (21) along the leading screw (19) axially-movable.
4. magnetic bionanoparticles method as claimed in claim 2 extracts serum-virus RNA kits, it is characterised in that the card
The side of the close gripper shoe (18) laterally opens up the mounting groove (25) for gripper shoe described in clamping (18) on head (21).
5. magnetic bionanoparticles method as claimed in claim 4 extracts serum-virus RNA kits, it is characterised in that described
The end of fagging (18) is connected in the mounting groove (25), and the mounting groove (25) top sidewall is worn and connect for fixed described
The bolt (26) of gripper shoe (18).
6. magnetic bionanoparticles method as claimed in claim 2 extracts serum-virus RNA kits, it is characterised in that the appearance
Receive and be placed with some ice bags (27) positioned at the base plate (17) bottom in chamber (4), on the base plate (17) through hole is uniformly provided with.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621121683.7U CN206089661U (en) | 2016-10-13 | 2016-10-13 | Biological nanometer paramagnetic particle method extracts serum virus RNA kit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621121683.7U CN206089661U (en) | 2016-10-13 | 2016-10-13 | Biological nanometer paramagnetic particle method extracts serum virus RNA kit |
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| Publication Number | Publication Date |
|---|---|
| CN206089661U true CN206089661U (en) | 2017-04-12 |
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| CN201621121683.7U Active CN206089661U (en) | 2016-10-13 | 2016-10-13 | Biological nanometer paramagnetic particle method extracts serum virus RNA kit |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108165465A (en) * | 2018-03-27 | 2018-06-15 | 成都曌昊科技有限公司 | A kind of acquisition process kit for acquisition stem cell in tooth |
| CN108624477A (en) * | 2018-06-27 | 2018-10-09 | 盛蕾 | A kind of kit of detection bacterium vaginosis |
| CN113640196A (en) * | 2021-02-03 | 2021-11-12 | 深圳市帝迈生物技术有限公司 | Kit and POCT blood cell analyzer |
-
2016
- 2016-10-13 CN CN201621121683.7U patent/CN206089661U/en active Active
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108165465A (en) * | 2018-03-27 | 2018-06-15 | 成都曌昊科技有限公司 | A kind of acquisition process kit for acquisition stem cell in tooth |
| CN108624477A (en) * | 2018-06-27 | 2018-10-09 | 盛蕾 | A kind of kit of detection bacterium vaginosis |
| CN113640196A (en) * | 2021-02-03 | 2021-11-12 | 深圳市帝迈生物技术有限公司 | Kit and POCT blood cell analyzer |
| CN113640196B (en) * | 2021-02-03 | 2023-12-01 | 深圳市帝迈生物技术有限公司 | Kit and POCT blood cell analyzer |
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| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20180423 Address after: 100096 room 916, King Yan Long building, Hui lung Guan Town, Changping District, Beijing. Patentee after: Beijing nine Lianhua Culture Communication Co., Ltd. Address before: 100098 Beijing city Haidian District Dazhongsi No. 13 Building No. 1 hospital basement A02-28 Patentee before: The state of Beijing Rongzhi Biological Technology Co. Ltd. |
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| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20210324 Address after: 102200 1101, building 3, No.1 a, Chaoqian Road, science and Technology Park, Changping District, Beijing Patentee after: Beijing Jinyuan Liheng Biotechnology Co.,Ltd. Address before: 100096 room 916, King Yan Long building, Hui lung Guan Town, Changping District, Beijing. Patentee before: Beijing nine Lianhua Culture Communication Co.,Ltd. |
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| TR01 | Transfer of patent right |