CN206345846U

CN206345846U - A kind of magnetic bionanoparticles method PCR primer purification kit

Info

Publication number: CN206345846U
Application number: CN201621125943.8U
Authority: CN
Inventors: 张金菊; 王红光
Original assignee: State Of Beijing Rongzhi Biological Technology Co Ltd
Current assignee: State Of Beijing Rongzhi Biological Technology Co Ltd
Priority date: 2016-10-14
Filing date: 2016-10-14
Publication date: 2017-07-21
Anticipated expiration: 2026-10-14

Abstract

The utility model is related to a kind of magnetic bionanoparticles method PCR primer purification kit, lid including box body and above box body, plate is provided with box body, plate is provided with multiple storage tanks for being contained with reagent bottle, the bottom of lid is provided with multigroup neck, every group of neck is made up of removably clamping finite place piece, locating part clamp and the limited post being connected on clamp；Clamp is connected in neck just, and spacing column bottom has stopper slot, when covering lid, and the stopper slot of limited post inserts the top of correspondence reagent bottle just.The reagent cartridge configuration is simple, novel in design, carries out PCR primer purifying specifically designed for the sample of particular source, can be supplied to the fields such as the scientific research or inspection and quarantine of different demands and use.

Description

A kind of magnetic bionanoparticles method PCR primer purification kit

Technical field

The utility model belongs to biological technical field, more particularly to a kind of magnetic bionanoparticles method PCR primer purified reagent Box.

Background technology

Since magnetic separation technique based on micro-nano magnetic bead is born from the eighties in last century, life science and face More and more applied in the fields such as bed detection.Beads enrichment technical operation is simple, and strong adaptability was both manually operable, Automation equipment can be applied to very well to operate.Magnetic bionanoparticles be one by Microbe synthesis magnetic nanoparticle, be natural Biomagnetism nano material, with the incomparable advantage of some artificial synthesized magnetic beads.Utilize technique for gene engineering or knot Chemical modification technology is closed, special biologically functional molecule can be shown in magnetic bionanoparticles surface orientation, or be coated with other materials Such as silylation material so that magnetic bionanoparticles can be used in the purifying and extraction of common nucleic acid and specific nucleic acid molecule.

Paramagnetic particle method, which extracts sample of nucleic acid, has important application value in life science and field of medical examination, existing to be directed to Its DNA nucleic acid compositions of the sample extraction of separate sources need to use different processing methods, develop maturation, standard, operation side Just kit.The kit as disclosed in patent 201620303318.1,201620303202.8 etc., detection reagent difference is vertical It is contained in different cavitys, classify storage, access is convenient, so as to improve user's scientific research, detect the efficiency of work.

But available reagent box such as is likely to occur inversion, dropped at inappropriate operation or the accident during transport, use, Easily break reagent bottle, cause the pollution and waste of whole kit.For the situation, applicant is thought in available reagent box On the basis of, urgent need is improved, and improves the shockproof drop resistant ability of kit.

Utility model content

In order to solve the above-mentioned technical problem, the utility model provides a kind of magnetic bionanoparticles method PCR primer purified reagent Box, with simple in construction, modern design, the splendid advantage of practicality, and the locating part of kit is freely adjustable to adapt to The reagent bottle that length differs, the integral assembling of kit changes more flexible.

The utility model concrete technical scheme is as follows：

The utility model provides a kind of magnetic bionanoparticles method PCR primer purification kit, including box body and located at box body Plate is provided with the lid of top, the box body, the plate is provided with multiple storage tanks for being contained with reagent bottle, the lid Bottom be provided with multigroup neck, neck described in every group by removably clamping finite place piece, the locating part by clamp and The limited post being connected on the clamp is constituted；The clamp is connected in the neck just, and the spacing column bottom has Stopper slot, when covering lid, the stopper slot of the limited post inserts the top of correspondence reagent bottle just.

In the further scheme of the utility model, the height of limited post is 0.8-1.5cm, and the depth of groove of the stopper slot is 0.5-1cm。

In specific scheme, the limited post is prepared from by sponge material, and the outer side edges of the limited post are enclosed with modeling The fixture that material material is made.

Further, the stopper slot reinforcement of the inwall provided with annular, the further stable fixed reagent bottle.

A wherein preferred scheme is that the neck is made up of a pair of axial cross sections for the right angle bar of L-type, described in a pair The transverse bar body of right angle bar lower end is oppositely arranged

In further technical scheme, the top of the box body side is provided with least two jacks, and the jack depth is 2-5cm；The bottom of the lid side is provided with least two inserted links, a length of 1.8-4.8cm of inserted link；When the box body closes During lid, the inserted link and the jack coordinate, so that limit lid covers position, tester is made in lid more convenient Quickly.

In further one technical scheme, the quantity of the jack is two, and the jack is arranged on the box body The diagonal angle at top；The quantity for coordinating the inserted link of the jack is two, and the inserted link is arranged on the diagonal of the lid bottom Place.

In another preferred scheme, the bottom of the box body is provided with the first caulking groove extended laterally, first caulking groove Inside it is plugged with provided with the placement plate for placing magnetic means and/or operation instrument in drawer, the drawer, the placement plate is provided with Multiple and magnetic means and/or operation instrument concrete form identical depressed part.

In another preferred scheme, the second caulking groove extended laterally, described second are additionally provided with below first caulking groove The side of lower section two inside draw back plate, second caulking groove is provided with caulking groove and is provided with chute, the two bottom sides of the draw back plate are set There is the sliding block being engaged with chute, the draw back plate is provided with writing area, and the writing area includes minute book and pen.

The magnetic bionanoparticles method PCR primer purification kit of the present invention is described to hold in a specific preferred scheme The quantity for putting groove is 5, and the correspondence limited post quantity is 5；The lyophilized biology of storage that the form that the reagent bottle includes differs The reagent bottle of nanometer magnetic bead reagent, the reagent bottle for depositing freeze-dried reagent dilution buffer, storage PCR primer handle the examination of buffer solution Agent bottle, the reagent bottle of storage lavation buffer solution and the reagent bottle for depositing Dnase/Rnase-Free elution buffers.

The utility model magnetic bionanoparticles method PCR primer purification kit is simple in construction, modern design, practicality pole It is good.The locating part of kit is that dismantled and assembled form is configured, while the Design of length of locating part and its stopper slot is enough to length, Its freely adjustable locating part is set to adapt to the reagent bottle that length differs；The setting of locating part makes reagent bottle be stably fixed on reagent , will not be because being damaged colliding, extrude by external force etc. in box；The limited post of sponge material is in reagent bottle cap because covering not It is close to cause to adsorb diffusate during extravasation, it is to avoid pollution kit.In addition, this kit is also accompanied with magnetic means and/or behaviour Make instrument, to detect the facility brought great convenience；And write area and provide easily detection record operation for tester.

Brief description of the drawings

Fig. 1 is the structural representation of the first embodiment of magnetic bionanoparticles method PCR primer purification kit；

Fig. 2 is the first embodiment structural representation of the lid of magnetic bionanoparticles method PCR primer purification kit；

Fig. 3 is the partial enlargement of the first embodiment of the lid of magnetic bionanoparticles method PCR primer purification kit Figure；

Fig. 4 is the partial enlargement of second of embodiment of the lid of magnetic bionanoparticles method PCR primer purification kit Figure；

Fig. 5 is the structural representation of the third embodiment of the box body of magnetic bionanoparticles method PCR primer purification kit Figure；

Fig. 6 is the structural representation of the third embodiment of the lid of magnetic bionanoparticles method PCR primer purification kit Figure.

Embodiment

With reference to the accompanying drawings and examples, embodiment of the present utility model is further described.Implement below Example is only used for clearly illustrating the technical solution of the utility model, and protection model of the present utility model can not be limited with this Enclose.

Fig. 1-3 shows a kind of magnetic bionanoparticles method PCR primer purification kit of the present utility model, including box body 1 With the lid 2 located at the top of box body 1, it is provided with plate 3 in the box body 1, the plate 3 is provided with multiple reagent bottles that are contained with Storage tank, the bottom of the lid 2 is provided with multigroup neck 20, and neck 20 described in every group passes through removably clamping finite place piece 4, the locating part 4 is made up of clamp 40 and the limited post 41 being connected on the clamp 40；The clamp 40 is connected to just In the neck 20, the bottom of limited post 41 has stopper slot 42, when covering lid 2, the stopper slot of the limited post 41 42 insert the top of correspondence reagent bottle just.The height of wherein described limited post 41 be 0.8-1.5cm, the stopper slot 42 it is recessed Groove depth is 0.5-1cm.The neck 20 is made up of a pair of axial cross sections for the right angle bar 21 of L-type, right angle bar 21 described in a pair The transverse bar body of lower end is oppositely arranged.

Fig. 4 shows second of embodiment of the utility model magnetic bionanoparticles method PCR primer purification kit, In above-mentioned embodiment, in addition to specific following technical characteristic：The limited post 41 is prepared from by sponge material, the limit The outer side edges of position post 41 are enclosed with the fixture 43 that plastic material is made.Reinforcement of the inwall of the stopper slot 42 provided with annular 44。

The top of the side of box body 1 of the utility model kit is provided with least two jacks 10, and the depth of jack 10 is 2-5cm；The bottom of the side of lid 2 is provided with least two inserted links 5, a length of 1.8-4.8cm of the inserted link 5；When the box body 1 When closing lid 2, the inserted link 5 coordinates with the jack 10, so that limit lid 2 covers position.

Fig. 5-6 shows the preferred embodiment of a lid, and the quantity of the jack 10 is two, and the jack 10 is set Diagonal angle at the top of box body 1；The quantity for coordinating the inserted link 5 of the jack 10 is two, and the inserted link 5 is arranged on described The diagonal angle of the bottom of lid 2.

Fig. 5-6 shows second of embodiment of the utility model magnetic bionanoparticles method PCR primer purification kit, institute The bottom for stating box body 1 is provided with to be plugged with drawer 7, the drawer 7 in the first caulking groove 6 for extending laterally, first caulking groove 6 and set There is the placement plate 70 for placing magnetic means and/or operation instrument, the placement plate 70 is provided with multiple and magnetic means and/or behaviour Make instrument concrete form identical depressed part 71.

The lower section of first caulking groove 4, which is additionally provided with the second caulking groove 8 extended laterally, second caulking groove 8, is provided with draw back plate 9, the side of lower section two inside second caulking groove 8 is provided with chute, and the two bottom sides of the draw back plate 9 with chute provided with being engaged Sliding block, the draw back plate 9 provided with writing area 90, the writing area 90 includes minute book and pen.

The quantity of storage tank of the present utility model is 5, and the correspondence quantity of limited post 41 is 5；The reagent bottle bag The reagent bottle that includes the lyophilized magnetic bionanoparticles reagent of storage that form differs, the reagent bottle for depositing freeze-dried reagent dilution buffer, The reagent bottle for depositing PCR primer processing buffer solution, the reagent bottle and storage Dnase/Rnase-Free of depositing lavation buffer solution The reagent bottle of elution buffer.

Claims

1. a kind of magnetic bionanoparticles method PCR primer purification kit, including box body (1) and the lid above box body (1) (2) plate (3), is provided with the box body (1), the plate (3) is provided with multiple storage tanks for being contained with reagent bottle, its feature It is, the bottom of the lid (2) is provided with multigroup neck (20), and neck (20) is limited by removably clamping described in every group Position part (4), the locating part (4) is made up of clamp (40) and the limited post (41) being connected on the clamp (40)；The card Plate (40) is connected in the neck (20) just, and limited post (41) bottom has stopper slot (42), when covering lid (2) When, the stopper slot (42) of the limited post (41) inserts the top of correspondence reagent bottle just.

2. magnetic bionanoparticles method PCR primer purification kit as claimed in claim 1, it is characterised in that the limited post (41) height is 0.8-1.5cm, and the depth of groove of the stopper slot (42) is 0.5-1cm.

3. magnetic bionanoparticles method PCR primer purification kit as claimed in claim 1, it is characterised in that the limited post (41) it is prepared from by sponge material, the outer side edges of the limited post (41) are enclosed with the fixture (43) that plastic material is made.

4. magnetic bionanoparticles method PCR primer purification kit as claimed in claim 3, it is characterised in that the stopper slot (42) reinforcement (44) of the inwall provided with annular.

5. magnetic bionanoparticles method PCR primer purification kit as claimed in claim 1, it is characterised in that the neck (20) be made up of a pair of axial cross sections for the right angle bar (21) of L-type, the transverse bar body phase of right angle bar (21) lower end described in a pair to Set.

6. magnetic bionanoparticles method PCR primer purification kit as claimed in claim 1, it is characterised in that the box body (1) The top of side is provided with least two jacks (10), and jack (10) depth is 2-5cm；The bottom of lid (2) side Provided with least two inserted links (5), a length of 1.8-4.8cm of the inserted link (5)；It is described when the box body (1) closes lid (2) Inserted link (5) and the jack (10) coordinate, so that limit lid (2) covers position.

7. magnetic bionanoparticles method PCR primer purification kit as claimed in claim 6, it is characterised in that the jack (10) quantity is two, and the jack (10) is arranged on the diagonal angle at the top of the box body (1)；Coordinate the jack (10) The quantity of inserted link (5) is two, and the inserted link (5) is arranged on the diagonal angle of the lid (2) bottom.

8. magnetic bionanoparticles method PCR primer purification kit as claimed in claim 1, it is characterised in that the box body (1) Bottom be provided with the first caulking groove (6) for extending laterally, first caulking groove (6) and be plugged with drawer (7), in the drawer (7) Provided with the placement plate (70) for placing magnetic means and/or operation instrument, the placement plate (70) is provided with multiple and magnetic means And/or operation instrument concrete form identical depressed part (71).

9. magnetic bionanoparticles method PCR primer purification kit as claimed in claim 8, it is characterised in that described first is embedding It is additionally provided with below groove (6) in the second caulking groove (8) extended laterally, second caulking groove (8) and is provided with draw back plate (9), described second The internal side of lower section two of caulking groove (8) is provided with chute, and the two bottom sides of the draw back plate (9) are provided with the cunning being engaged with chute Block, the draw back plate (9) includes minute book and pen provided with writing area (90), the writing area (90).

10. magnetic bionanoparticles method PCR primer purification kit as claimed in claim 1, it is characterised in that the storage tank Quantity be 5, correspondence limited post (41) quantity is 5；The lyophilized biology of storage that the form that the reagent bottle includes differs The reagent bottle of nanometer magnetic bead reagent, the reagent bottle for depositing freeze-dried reagent dilution buffer, storage PCR primer handle the examination of buffer solution Agent bottle, the reagent bottle of storage lavation buffer solution and the reagent bottle for depositing Dnase/Rnase-Free elution buffers.