CN205993154U - Mycorrhizal fungi locellus culture apparatus - Google Patents
Mycorrhizal fungi locellus culture apparatus Download PDFInfo
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- CN205993154U CN205993154U CN201620893989.8U CN201620893989U CN205993154U CN 205993154 U CN205993154 U CN 205993154U CN 201620893989 U CN201620893989 U CN 201620893989U CN 205993154 U CN205993154 U CN 205993154U
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- phytotron
- mycelial growth
- mycorrhizal fungi
- plant
- culture apparatus
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Abstract
The utility model discloses a kind of mycorrhizal fungi locellus culture apparatus, belong to field of microbial culture technology, a kind of simple structure is provided, and can achieve the mycorrhizal fungi locellus culture apparatus of the locellus culture to mycorrhizal fungi, including mycelial growth frame, it is mycelial growth room in the mycelial growth inframe, the plant growth frame being detachably connected is provided with least side of the mycelial growth frame, it is phytotron in the plant growth inframe, the mycelial growth room is connected with the phytotron, and the separate mesh that root system of plant can be allowed to pass through is provided between phytotron and mycelial growth room.The utility model when mycorrhizal fungi is cultivated, in the case that a small amount of inoculation microbial inoculum is provided, you can obtain the mixtures such as substantial amounts of mycorhiza, spore, mycelia；It is more prone to can the acquisition of microbial inoculum.And, for the collection of the mixtures such as mycorhiza, spore, mycelia, mycelial growth frame can be directly dismantled by carrying out blocking after separation along separate mesh.
Description
Technical field
The utility model is related to field of microbial culture technology, more particularly to a kind of mycorrhizal fungi locellus culture apparatus.
Background technology
Mycorhiza is a kind of plant with mycorrhizal fungi in long-term survival processes, a kind of symbiosis that common evolutionary goes out.
Mycorrhizal fungi can improve absorption of the host to mineral element and moisture, and can effectively improve the survival ability of plant.And it is some
Mycorrhizal fungi has larger economic worth and nutritive value, such as ferfas (Tuber), matsutake (Tricholoma
Matsutake), cepe (Boletus edulis) etc..But as mycorrhizal fungi is difficult to depart from host's cultured in vitro.
While some precious mushrooms, such as French black truffle (Tuber melanosporum), the white ferfas (Tuber of Italy
Magnatum) etc., because its preciousness is be difficult to large-scale inoculation at home.Therefore, being trained for bacterium of VA Mycorrhizal Fungi
The production of mycorrhiza fungi microbial inoculum and an important restrictive condition of research.At present, biological technology of mycorrhiza fails always in agriculture field
To use is widely popularized, it is exactly that pure strain inoculation microbial inoculum is difficult to obtain to trace it to its cause.
Utility model content
The technical problem that the utility model is solved is to provide a kind of simple structure, and can achieve the locellus training to mycorrhizal fungi
Foster mycorrhizal fungi locellus culture apparatus, the utility model can obtain substantial amounts of bacterium in the case of a small amount of inoculation microbial inoculum is provided
The mixtures such as root, spore, mycelia, and this mixture can be used as microbial inoculum use so that the acquisition of microbial inoculum is more facilitated.
The utility model solves the technical scheme adopted by its technical problem：Mycorrhizal fungi locellus culture apparatus, including
Mycelial growth frame, is mycelial growth room in the mycelial growth inframe, and being provided with least side of the mycelial growth frame can
The plant growth frame of dismounting connection, is phytotron, the mycelial growth room and the plant in the plant growth inframe
Growth room connects, and is provided with the separate mesh that root system of plant can be allowed to pass through between phytotron and mycelial growth room.
It is further：The middle part of the phytotron is provided with screen cloth, phytotron is divided into by the screen cloth
Upper chamber and lower chambers；Wherein described screen cloth can allow fungal spore to pass through, and root system of plant can be prevented to pass through.
It is further：It is provided with a metal bar between phytotron and mycelial growth room, the one of the screen cloth
While being connected on the metal bar.
It is further：The screen cloth is metallic sieve, and the screen-aperture of screen cloth is 30 μm.
It is further：Bottom chamber in plant growth room is provided with swash plate, and lower chambers are divided into by the swash plate
Half chamber and lower half chamber, and the base of the swash plate is located at the base joint of phytotron and mycelial growth room, institute
The top margin for stating swash plate is located at the joint of phytotron and screen cloth.
It is further：The gradient of the swash plate is 45 °.
It is further：The separate mesh is nylon wire, and the mesh aperture of nylon wire is 1mm.
It is further：Plant culture is provided with epicoele room, the plant culture is pressed with vermiculite by peat soil
1：1 volume ratio is mixed to get；Cultural hypha base is provided with lower chambers and mycelial growth room, and the cultural hypha base is by river
Sand presses 1 with vermiculite：1 volume ratio is mixed to get.
It is further：Plant culture is provided with epicoele room, the plant culture is pressed with vermiculite by peat soil
1：1 volume ratio is mixed to get；Cultural hypha base, the mycelia are provided with the upper half chamber of lower chambers and mycelial growth room
Culture medium presses 1 by river sand with vermiculite：1 volume ratio is mixed to get；It is vacant setting in the lower half within the chamber of lower chambers.
It is further：The plant growth being detachably connected is respectively arranged with the left and right sides of the mycelial growth frame
Frame.
The beneficial effects of the utility model are：The utility model simple structure, and easy to use, can improve to mycorrhizal fungi
Culture efficiency.And mycelial growth room is separated by separate mesh with phytotron, so can be prevented effectively from because of mycelia
Growth room is different from the growth substrate in plant growth room and avoids allogenic material from polluting mycorrhizal fungi, and then effectively
The normal growth that ensure that mycorrhizal fungi；And, can pass through when mycorhiza, spore and the mycelia of mycelial growth inframe is collected
Carry out blocking separation along separate mesh, then directly dismantle mycelial growth frame, and then very easily can obtain substantial amounts of mycorhiza,
The mixtures such as spore, mycelia.In addition, further by being arranged on the screen cloth of phytotron bottom, can effectively collect mycorhiza true
Bacterium spore, the aperture of screen cloth be set to sufficiently small so that root system cannot grow downwards, while allow mycorrhizal fungal spore to lead to again
Cross, and screen cloth can also avoid the excessive waterlogging problem caused by root system of watering.The utility model can be obtained in mycelial growth room
Obtain the mixture such as substantial amounts of mycorhiza, spore, mycelia；The detection of mycorhiza is facilitated, more can easily be obtained and substantial amounts of be inoculated with bacterium
Agent, reduces experimental cost.
Description of the drawings
Fig. 1 is a kind of 3-D view of instantiation of mycorrhizal fungi locellus culture apparatus described in the utility model；
Fig. 2 is the top view of example shown in Fig. 1；
Fig. 3 is the sectional view of Section A-A in Fig. 2；
In figure is labeled as：Mycelial growth frame 1, mycelial growth room 2, plant growth frame 3, phytotron 4, upper chamber 41,
Lower chambers 42, upper half chamber 421, lower half chamber 422, separate mesh 5, screen cloth 6, metal bar 7, swash plate 8.
Specific embodiment
With reference to the accompanying drawings and detailed description the utility model is further illustrated.
Mycorrhizal fungi locellus culture apparatus described in the utility model, including mycelial growth frame 1, in the mycelial growth frame
Be mycelial growth room 2 in 1, the plant growth frame 3 being detachably connected is provided with least side of the mycelial growth frame 1,
It is phytotron 4 in the plant growth frame 3, the mycelial growth room 2 is connected with the phytotron 4, and is being planted
The separate mesh 5 that root system of plant can be allowed to pass through is provided between thing growth room 4 and mycelial growth room 2.Wherein, mycelial growth room 2
It is the space for mycorrhizal fungi growth, can be used to cultivate the such as all kinds of mycorrhizal fungis such as ferfas, matsutake, cepe；And plant
Thing growth room 4 is then that Huashan pine, oak that Common Cultivation is grown beneficial to mycorrhizal fungi etc. are posted for cultivating the space of corresponding plant
Main plant.In specific embodiment as shown in Figure 1 to Figure 3, be respectively arranged with the left and right sides of mycelial growth frame 1 removable
Unload the plant growth frame 3 of connection.
Wherein, mycelial growth room 2 is connected with the phytotron 4, refer in mycelial growth frame 1 and plant growth frame
The one side of 3 mutual splicings is all not provided with corresponding baffle arrangement so that the two internal phytotron 4 and mycelial growth room
2 is connected state, to allow root system of plant to grow into mycelial growth room 2 from phytotron 4；Such mycorrhizal fungi
Combined with the root system in mycelial growth room 2 is grown into, and mycorhiza is formed in mycelial growth room 2.And the utility model is further
Dismountable connected mode will be set between mycelial growth frame 1 and plant growth frame 3, so, collect mycelial growth afterwards
Only need to during mycorhiza in room 2 mycelial growth frame 1 is individually separated along segmentation net 5.
In addition, arranging the effect of separate mesh 5, it is mainly used for intercepting mycelial growth room 2 and the culture in phytotron 4
Matrix, because generally, the matrix placed in mycelial growth room 2 and phytotron 4 is simultaneously differed, plant culturing room
The culture matrixes such as the organoclay being typically filled in 4 beneficial to plant growth, and river sand etc. is then typically filled in mycelial growth room 2
Matrix.Certainly, separate mesh 5 should be set to intercept root system of plant and give birth to mycelial growth room 2 from phytotron 4
Long, therefore the mesh size to separate mesh 5 has certain requirement, that is, require which can allow root system of plant to pass through, while can rise again
Arrive the barriering effect to both sides different substrates.Certainly, for the root system of different plants, different separate mesh 5 can be set in theory
Mesh size.More particularly, separate mesh 5 can be set for nylon wire, and the mesh aperture of nylon wire can be set further and be
1mm, so can substantially meet the requirement of various plants root system.
In addition, also screen cloth 6 can be provided with the middle part of the phytotron 4, the screen cloth 6 is by 4 points of phytotron
For upper chamber 41 and lower chambers 42；Wherein described screen cloth 6 can allow fungal spore to pass through, and root system of plant can be prevented to pass through.Sieve
The effect of net 6 mainly has at following 2 points：First, it is to allow fungal spore to pass through, the more conducively collection to fungal spore, and can keep away
Exempt from root system of plant to grow further downward, and promote root system of plant to grow into the mycelial growth room 2 of side；Second, it is to keep away
Exempt to water excess when cause the situation of waterlogging to root system of plant, it can be ensured that effective growth of root system of plant.In addition, for screen cloth 6,
Metallic sieve, and size cases according to spore specifically may be configured as, and the screen-aperture that can arrange screen cloth 6 further is 30 μm
Left and right.
Certainly, it is contemplated that between phytotron 4 and mycelial growth room 2 in addition to being provided with separate mesh 5, itself
For connectivity structure, therefore, when screen cloth 6 is provided with, in order to play certain support effect to screen cloth 6, can further in this place
A piece metal bar 7 is set, as it is shown in the figures, so being connected to the screen cloth 6 on the metal bar 7, by gold
Category rod 7 plays support effect to screen cloth 6.Certainly, the structural strength of metal bar 7 should meet requirement, can typically arrange metal bar 7
A diameter of 1mm-5mm.
In addition, also swash plate 8 can be provided with lower chambers 42 further in the phytotron 4, the swash plate 8 is by cavity of resorption
Room 42 is divided into upper half chamber 421 and lower half chamber 422, and the base of the swash plate 8 is located at phytotron 4 and mycelial growth
The base joint of room 2, the top margin of the swash plate 8 are located at the joint of phytotron 4 and screen cloth 6.Wherein, swash plate 8 is set
Effect be mainly used for making the spore that top produces, through after screen cloth 6, can flow back and collect in mycelial growth room 2, more
It is beneficial to form mycorhiza in mycelial growth room 2.Therefore, by the setting using above-mentioned swash plate 8, so that positioned at 8 top of swash plate
Upper half chamber 421 can be while connect the upper chamber 41 of top with the mycelial growth room 2 of side.General, swash plate 8 can be set
Gradient is 45 ° or so, certainly, arranges other amount of tapers also dependent on actual conditions.Certainly, swash plate 8 may also be employed other tools
The bent plate structure for having the effect above is realized.In addition, the lower half chamber 422 below swash plate 8 is generally vacant setting, can not make
With.
In the incubation of actual mycorrhizal fungi, typically in phytotron 4, plant culture is provided with, the plant
Thing culture medium presses 1 by peat soil with vermiculite：1 volume ratio is mixed to get；And cultural hypha is provided with mycelial growth room 2
Base, the cultural hypha base press 1 by river sand with vermiculite：1 volume ratio is mixed to get.General, in order to avoid causing to fungi
Pollution, above-mentioned peat soil, vermiculite, river sand etc., using be front required to through high-temperature heat sterilization process：Treatment temperature is generally
121～126 DEG C, handling duration is 2h or so.In addition, when screen cloth 6 is provided with, plant culture only can be arranged in upper chamber 41
Base, and cultural hypha base is set in lower chambers 42 and mycelial growth room 2.In addition, above-mentioned be provided with screen cloth 6 on the basis of,
When swash plate 8 is further provided with, cultural hypha base can be provided with the upper half chamber 421 of lower chambers 42 and mycelial growth room 2,
And be vacant setting in the lower half chamber 422 of lower chambers 42.In addition in incubation, can regularly nutrient solution, such as
Hogland nutrient solution etc., so can more effectively promote the growth of mycorrhizas homobium, more so as to obtain in fungi growth room
Mycorhiza.
Mycorrhizal fungi locellus culture apparatus described in the utility model, only needs in incubation provide a small amount of inoculation bacterium
Agent, can obtain the mixtures such as substantial amounts of mycorhiza, spore, mycelia in phytotron 4 and mycelial growth room 2 afterwards, with this be
A large amount of microbial inoculums can be obtained.
Claims (8)
1. mycorrhizal fungi locellus culture apparatus, it is characterised in that：Including mycelial growth frame (1), in mycelial growth frame (1)
For mycelial growth room (2), the plant growth frame (3) being detachably connected is provided with least side of mycelial growth frame (1),
It is phytotron (4) in plant growth frame (3), mycelial growth room (2) are connected with the phytotron (4)
Logical, and the separate mesh (5) that root system of plant can be allowed to pass through is provided between phytotron (4) and mycelial growth room (2).
2. mycorrhizal fungi locellus culture apparatus as claimed in claim 1, it is characterised in that：In the phytotron (4)
Middle part is provided with screen cloth (6), and phytotron (4) is divided into upper chamber (41) and lower chambers (42) by screen cloth (6)；Wherein institute
Stating screen cloth (6) can allow fungal spore to pass through, and root system of plant can be prevented to pass through.
3. mycorrhizal fungi locellus culture apparatus as claimed in claim 2, it is characterised in that：In phytotron (4) and mycelia
It is provided with a metal bar (7) between growth room (2), being connected on the metal bar (7) of screen cloth (6).
4. mycorrhizal fungi locellus culture apparatus as claimed in claim 2, it is characterised in that：Described screen cloth (6) are metallic sieve,
And the screen-aperture of screen cloth (6) is 30 μm.
5. mycorrhizal fungi locellus culture apparatus as claimed in claim 2, it is characterised in that：Under in the phytotron (4)
Swash plate (8) is provided with chamber (42), lower chambers (42) are divided into upper half chamber (421) and lower half chamber by swash plate (8)
(422), and the base of the swash plate (8) is located at the base joint of phytotron (4) and mycelial growth room (2), described
The top margin of swash plate (8) is located at the joint of phytotron (4) and screen cloth (6).
6. mycorrhizal fungi locellus culture apparatus as claimed in claim 5, it is characterised in that：The gradient of swash plate (8) is
45°.
7. mycorrhizal fungi locellus culture apparatus as claimed in claim 1, it is characterised in that：Described separate mesh (5) are nylon wire,
And the mesh aperture of nylon wire is 1mm.
8. the mycorrhizal fungi locellus culture apparatus as any one of claim 1 to 7, it is characterised in that：In the mycelia
The left and right sides of growth frame (1) is respectively arranged with the plant growth frame (3) being detachably connected.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620893989.8U CN205993154U (en) | 2016-08-17 | 2016-08-17 | Mycorrhizal fungi locellus culture apparatus |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620893989.8U CN205993154U (en) | 2016-08-17 | 2016-08-17 | Mycorrhizal fungi locellus culture apparatus |
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| CN205993154U true CN205993154U (en) | 2017-03-08 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105746098A (en) * | 2016-03-30 | 2016-07-13 | 四川省农业科学院土壤肥料研究所 | Ophiocordyceps sinensis spore collection device and collection method |
| CN108307926A (en) * | 2018-01-25 | 2018-07-24 | 北京林业大学 | A kind of arbuscular mycorrhizal fungi15N isotope labellings device and production method |
| CN117223542A (en) * | 2023-11-15 | 2023-12-15 | 山东金必来生物科技有限公司 | Arbuscular mycorrhizal fungus mycelium collector and collection method |
-
2016
- 2016-08-17 CN CN201620893989.8U patent/CN205993154U/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105746098A (en) * | 2016-03-30 | 2016-07-13 | 四川省农业科学院土壤肥料研究所 | Ophiocordyceps sinensis spore collection device and collection method |
| CN105746098B (en) * | 2016-03-30 | 2017-11-14 | 四川省农业科学院土壤肥料研究所 | Cordyceps sinensis spore collection method |
| CN108307926A (en) * | 2018-01-25 | 2018-07-24 | 北京林业大学 | A kind of arbuscular mycorrhizal fungi15N isotope labellings device and production method |
| CN117223542A (en) * | 2023-11-15 | 2023-12-15 | 山东金必来生物科技有限公司 | Arbuscular mycorrhizal fungus mycelium collector and collection method |
| CN117223542B (en) * | 2023-11-15 | 2024-02-23 | 山东金必来生物科技有限公司 | Arbuscular mycorrhizal fungus mycelium collector and collection method |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170308 Termination date: 20190817 |