CN205884428U - Survey medicine is to device of big bee mite toxicity - Google Patents
Survey medicine is to device of big bee mite toxicity Download PDFInfo
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- CN205884428U CN205884428U CN201620515325.8U CN201620515325U CN205884428U CN 205884428 U CN205884428 U CN 205884428U CN 201620515325 U CN201620515325 U CN 201620515325U CN 205884428 U CN205884428 U CN 205884428U
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- bee pupa
- bee
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- mite
- toxicity
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Abstract
The utility model discloses a survey medicine is to device of big bee mite toxicity. The device, including bee pupa cultivation board and bee pupa cultivation cup, wherein, if the board is cultivateed to the bee pupa is the dry hole, the size in hole is corresponding with bee pupa cultivation cup, and every kong ke holds a bee pupa cultivation cup, bee pupa cultivation cup is the cup body, filter paper is laid to bee pupa cultivation cup's bottom, and the rim of a cup is established and is used for the ventilative transparent film that seals. Does the structural design of above -mentioned device provide the culture vessel of larva to the bee pupa bee pupa cultivation cup, simultaneously, medicine that this container can also insert big bee mite and have different toxicity to big bee mite for, detect and more be close natural state, it is more lifelike. Simultaneously, the device has still made things convenient for multi -sample's detection, utilizes porous bee pupa to cultivate board 1, makes once to detect and to carry out tens repetitions, is fit for the experiment that toxicity detected the big sample test of this kind of needs.
Description
Technical field
The utility model is related to a kind of mensure device to varoa mite toxicity for the medicine.
Background technology
Honeybee is important economic insects, has very big economic worth and the ecological value, and honeybee can provide beneficial
Bee product can be pollinated for plant again, and varoa mite is the honeybee harmful animal of the most serious of harm apiculture.
Varoa mite is the honeybee harmful animal of the most serious of harm world's apiculture, due to Long-Time Service pyrethroid mite killing
Agent, varoa mite creates the serious resistance to the action of a drug, and the acaricidal screening of new and effective green is extremely urgent.And lack in the world and kill
The standard method to varoa mite toxicity test for the mite agent.At present, typically two methods are adopted, a kind of is bee colony toxicity test method, separately
A kind of is Toxicity Determination method.Both approaches all have the shortcomings that certain it is impossible to the poison to varoa mite for the Accurate Determining acaricide
Power.Bee colony dispenser method: (1) uncontrollable experimental condition, affected by the factor such as environment and bee colony group's gesture larger, led to systematic error
Greatly；(2) reagent dosage is not the dosage of the actual contact of honeybee mite, leads to result inaccurate；(3) the honeybee mite between test bee colony is parasitic
Rate is inconsistent, leads to differ greatly in treatment group；(4) bee colony test is wasted time and energy.Toxicity Determination method is to access honeybee mite
Inwall is evenly coated with culture dish or the vial of medicine to be measured, measures the honeybee mite death rate；Or put into honeybee mite in culture dish, then
Spray medicine to be measured against honeybee mite, measure the honeybee mite death rate.The shortcoming of Toxicity Determination method is, because honeybee mite lacks food etc.
Factor, leads to the control group honeybee mite death rate high, result is insincere, also cannot measure the chronic toxicity to varoa mite for the medicine.
Utility model content
The technical problems to be solved in the utility model be provide that experiment condition is controlled, honeybee mite survival rate height, time-to-live length,
The result accurately believable mensure device to varoa mite toxicity for the medicine.
The mensure device to varoa mite toxicity for the medicine described in the utility model, including bee pupa culture plate and bee pupa culture
Cup it is characterised in that: if described bee pupa culture plate is to set dry hole, the size in described hole is corresponding to bee pupa culture cup, and each hole can
Accommodate a bee pupa culture cup；Described bee pupa culture cup is goblet, the bottom laying filter paper of described bee pupa culture cup, cup
Mouth sets the ventilative transparent membrane for sealing.
In an embodiment of the present utility model, described bee pupa culture plate sets 24-48 and can accommodate bee pupa culture cup
Hole.
Further, device of the present utility model also includes incubator, and during use, described bee pupa culture plate and bee pupa are cultivated
Cup is placed in incubator.
The structure design of said apparatus, there is provided the culture vessel of larva to bee pupa-bee pupa culture cup, meanwhile, this container
Varoa mite and the medicine to varoa mite with different toxicity can also be accessed so that, detection, closer to nature, is more forced
Very.Meanwhile, this device also facilitates the detection of multisample, using the bee pupa culture plate 1 of porous, so that one-time detection can be carried out several
Ten repetitions, the experiment of suitable toxicity detection this needs large sample detection.
Device of the present utility model can be used for acaricide and the acute toxicity of varoa mite measured and chronic toxicity mensure, utilizes
Device of the present utility model has the advantage that (1) experimental condition is controlled；(2) control group honeybee mite survival rate is high, the time-to-live
Long；(3) time saving and energy saving；(4) result is accurately credible.
The utility model is that screening miticide provides simple and effective test device, is substantially shorter green and kills honeybee mite
The research and development of medicine utilize the cycle, and existing important Research Significance has wide market prospects again.
Brief description
The side view that Fig. 1 measures the device to varoa mite toxicity for the medicine for the utility model (puts into the 24 of bee pupa culture cup
Hole bee pupa culture plate side view)；
Fig. 2 is bee pupa culture plate；
Fig. 3 is bee pupa culture cup.
Specific embodiment
According to the utility model, measure the device to varoa mite toxicity for the medicine, as Figure 1-3, including bee pupa culture plate 1
With bee pupa culture cup 2, if described bee pupa culture plate is to set dry hole, the size in described hole is corresponding to bee pupa culture cup, and each hole can
Accommodate a bee pupa culture cup；Described bee pupa culture cup is goblet, the bottom laying filter paper 4 of described bee pupa culture cup, cup
Mouth sets the ventilative transparent membrane 3 for sealing.
In an embodiment of the present utility model, described bee pupa culture plate sets 24-48 and can accommodate bee pupa culture cup
Hole.
Further, device of the present utility model also includes providing 34.5 DEG C of cultivation temperature, relative humidity to be 75%-
95% incubator.
The structure design of said apparatus, there is provided the culture vessel of larva to bee pupa-bee pupa culture cup, meanwhile, this container
Varoa mite and the medicine to varoa mite with different toxicity can also be accessed so that, detection, closer to nature, is more forced
Very.Meanwhile, this device also facilitates the detection of multisample, using the bee pupa culture plate 1 of porous, so that one-time detection can be carried out several
Ten repetitions, the experiment of suitable toxicity detection this needs large sample detection.
Embodiment
Embodiment 1, measure medicine using the utility model the poison to varoa mite for the medicine is measured to the device of varoa mite toxicity
Property
Prepare before test: apis mellifera (apis melifera) bee colony (healthy group infects honeybee mite group), incubator, ultra-clean
Platform, balance, magnetic stirring apparatus, mix oscillator, Hygrothermograph, pipettor, super-clean bench heater, cold light source lighting, spawning control
Device processed, Moving needle for bee-keeping, tweezers, 48 hole larva culture plates, larva culture cup, 24 hole bee pupa culture plates, bee pupa culture cup, honeybee mite is collected
Device (culture dish with cover), shifting mite small brushes, Parafilm, honeycomb incubation chamber, emgloves, mouth mask, royal jelly, glucose, really
Sugar, yeast extract, potassium sulfate, sodium chloride, distilled water, medicine to be measured.If no special instructions, the medicine used by the present embodiment and
Equipment all can obtain from commercial channels.
1 preparation honeybee mite
Check varoa mite parasitic rate in bee colony, the high bee colony of host age preference rate is to support mite group, does not control mite all the year round.Varoa mite poison
From foster mite group, during power on-test, take out the sub- spleen of new capping.Open lair one by one, collect varoa mite standby.
2 raising honeybee larvas
Control queen bee to lay eggs on an empty honeycomb with spawning controller, after 24h, discharge queen bee, with Moving needle for bee-keeping by children after 3d
Worm is transferred to equipped with the 48 warmed-up hole larva culture plates of man-made feeds, and every hole moves into a larva.Culture plate is placed into
In incubator, temperature is 34.5 DEG C, relative humidity 95%.Continuous feeding 6 days, the 1st day feeding 10 μ l feed a, feeding 10 in the 2nd day
μ l feed a, the 3rd day feeding 20 μ l feed b, the 4th day feeding 30 μ l feed c, the 5th day feeding 40 μ l feed c, the 6th day feeding 50 μ
L feed c, is transferred to bee pupa culture plate on the 7th day.
As shown in table 1, the percentage composition in table 1 is weight percentage to feed formula.
Table 1.
| Feed a | Feed b | Feed c | |
| Royal jelly | 44.25% | 42.95% | 50.00% |
| Glucose | 5.30% | 6.40% | 9.00% |
| Fructose | 5.30% | 6.40% | 9.00% |
| Yeast extract | 0.90% | 1.30% | 2.00% |
| Water | 44.25% | 42.95% | 30.00% |
Honeybee is from ovum to going out room (emergence) 21 days.4th day (1 day old larva) starts to support in laboratory, is larva training
Foster plate, the 10th day (capping larval phase), transfers to bee pupa culture plate, and being administered and connect mite is also this sky.Concrete operations see table 2.
Table 2.
3 dispensers
Measure medicine using the utility model and the toxicity to varoa mite for the medicine is measured to the device of varoa mite toxicity: by bee pupa
Culture cup 2 (Fig. 3) is put in 24 hole bee pupa culture plates 1 (Fig. 2), and a bee pupa culture cup (Fig. 1) is put in every hole.Medicine to be measured
It is configured to variable concentrations according to test requirements document, quantitative liquid medicine is dripped on the filter paper 4 got ready, filter paper all soaks, coating
Filter paper be placed in super-clean bench after solvent volatilization dry after, put in bee pupa culture cup 2, each culture cup a piece of paper
Piece, it is stand-by that bee pupa culture plate is placed into preheating in incubator (34.5 DEG C).Before larva goes to bee pupa culture plate 1, liquid is light
Then larva is transferred in bee pupa culture plate in larva culture plate by the body surface being gently sprayed on.Control group solvent is processed.
4 connect mite
Varoa mite is collected from the foster mite spleen preparing, in the bee pupa culture cup of each treatment group and control group, accesses one
Varoa mite, and seal culture hole (Fig. 3) with ventilative transparent membrane, prevent the varoa mite accessing from escaping from culture hole, be easy to observe
Honeybee mite survival condition.Bee pupa culture plate is put in incubator, temperature is 34.5 DEG C, relative humidity is 75%.
The 5 statistics honeybee mite death rates
Statistics 48h or 72h honeybee mite death toll, calculates the death rate, analyzes the acute toxicity to varoa mite for the medicine.Or connect daily
The survival condition of continuous record test honeybee mite, the time-to-live of every honeybee mite of statistics, analyzes the chronic toxicity to varoa mite for the medicine.
The honeybee mite number of the honeybee mite number/access of the death rate=death.
6th, the toxicity test to varoa mite for the Resistox (coumaphos)
Carry out the toxicity test to varoa mite for the Resistox (coumaphos) according to above-mentioned steps 1-5, each processes 3 weights
Multiple, often repeat 12 varoa mites, as shown in table 2, and d10 (i.e. capping larva the 1st day) chemicals treatment, it is concurrently accessed honeybee mite, after 48h
The statistics honeybee mite death rate.Concrete outcome is as shown in table 3.
Table 3.
In table 3,1ppm=0.001 ‰, percentage by weight.
7th, the chronic toxicity test to varoa mite for the Resistox (coumaphos)
Carry out the chronic toxicity test to varoa mite for the Resistox (coumaphos) according to above-mentioned steps 1-5, in such as table 2 institute
In the honeybee stage of development shown, honeybee develops the 10th day (i.e. capping larva the 1st day) and accesses honeybee mite, the 21st day (honeybee go out room or
Sprout wings) check the death rate.Concrete outcome is as shown in table 4.
Table 4.
In table 4,1ppm=0.001 ‰, percentage by weight.
Above-mentioned experiment shows, the advantage of the utility model method: (1) experimental condition is controlled；(2) control group honeybee mite survival rate
Height, the time-to-live is long；(3) time saving and energy saving；(4) result is accurately credible.
The method can be used for acaricide and the acute toxicity of varoa mite measured and chronic toxicity mensure, for screening miticide
Provide easy believable assay method, be substantially shorter the green research and development killing honeybee mite medicine and utilize the cycle, existing important
Research Significance have wide market prospects again.
The above is only preferred embodiment of the present utility model it is noted that ordinary skill people for the art
For member, on the premise of without departing from the utility model principle, some improvements and modifications can also be made, these improvements and modifications
Also should be regarded as protection domain of the present utility model.
Claims (3)
1. a kind of measure medicine to the device of varoa mite toxicity it is characterised in that: include bee pupa culture plate and bee pupa culture cup, institute
If stating bee pupa culture plate is to set dry hole, the size in described hole is corresponding to bee pupa culture cup, and each hole can accommodate a bee pupa culture
Cup；Described bee pupa culture cup is goblet, the bottom laying filter paper of described bee pupa culture cup, and rim of a cup sets ventilative for sealing
Transparent membrane.
2. device according to claim 1 it is characterised in that: described bee pupa culture plate sets 24-48 and can accommodate bee pupa training
The hole of foster cup.
3. device according to claim 1 it is characterised in that: described device also includes incubator, during use, described bee pupa
Culture plate and bee pupa culture cup are placed in incubator.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620515325.8U CN205884428U (en) | 2016-05-31 | 2016-05-31 | Survey medicine is to device of big bee mite toxicity |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620515325.8U CN205884428U (en) | 2016-05-31 | 2016-05-31 | Survey medicine is to device of big bee mite toxicity |
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| Publication Number | Publication Date |
|---|---|
| CN205884428U true CN205884428U (en) | 2017-01-18 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201620515325.8U Withdrawn - After Issue CN205884428U (en) | 2016-05-31 | 2016-05-31 | Survey medicine is to device of big bee mite toxicity |
Country Status (1)
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| CN (1) | CN205884428U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105850898A (en) * | 2016-05-31 | 2016-08-17 | 中国农业科学院蜜蜂研究所 | Method for measuring acute and chronic toxicity of drug against varroa jacobsoni |
| CN114304070A (en) * | 2021-12-13 | 2022-04-12 | 中国农业科学院蜜蜂研究所 | Method and device for detecting bee mite infection rate |
-
2016
- 2016-05-31 CN CN201620515325.8U patent/CN205884428U/en not_active Withdrawn - After Issue
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105850898A (en) * | 2016-05-31 | 2016-08-17 | 中国农业科学院蜜蜂研究所 | Method for measuring acute and chronic toxicity of drug against varroa jacobsoni |
| CN105850898B (en) * | 2016-05-31 | 2018-10-12 | 中国农业科学院蜜蜂研究所 | A method of drug is measured to the acute and chronic toxicity of varoa mite |
| CN114304070A (en) * | 2021-12-13 | 2022-04-12 | 中国农业科学院蜜蜂研究所 | Method and device for detecting bee mite infection rate |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| AV01 | Patent right actively abandoned | ||
| AV01 | Patent right actively abandoned | ||
| AV01 | Patent right actively abandoned |
Granted publication date: 20170118 Effective date of abandoning: 20181012 |
|
| AV01 | Patent right actively abandoned |
Granted publication date: 20170118 Effective date of abandoning: 20181012 |