CN110045104A - A kind of the toxicity test device and application method of trypetid class adult stomach toxicity - Google Patents
A kind of the toxicity test device and application method of trypetid class adult stomach toxicity Download PDFInfo
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- CN110045104A CN110045104A CN201910407444.XA CN201910407444A CN110045104A CN 110045104 A CN110045104 A CN 110045104A CN 201910407444 A CN201910407444 A CN 201910407444A CN 110045104 A CN110045104 A CN 110045104A
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- 238000000034 method Methods 0.000 title claims abstract description 16
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/5014—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing toxicity
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5091—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing the pathological state of an organism
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2430/00—Assays, e.g. immunoassays or enzyme assays, involving synthetic organic compounds as analytes
- G01N2430/10—Insecticides
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Abstract
The present invention relates to the toxicity test devices and application method of a kind of trypetid class adult stomach toxicity, and the inventive system comprises toxicity test tanks and adult receiving flask；Toxicity test tank includes the cover and tank body that top is arranged in, and ventilating mechanism A is provided in the middle part of cover；It is provided in tank base into worm channel and objective table；The bottleneck of adult receiving flask is connect by entering worm channel with toxicity test tank.Structure of the invention accuracy that is ingenious, easy to use, low in cost, being remarkably improved labor efficiency reduction labor intensity, raising toxicity test.
Description
Technical field
The invention belongs to insect virulence assay device structures and toxicity test method and technology field, refer specifically to be exclusively used in trypetid
The stomach toxicity toxicity test device of this insect of class adult and toxicity test method and technology field.
Background technique
Tephritidae (Tephritidae) is subordinate to Insecta (Insecta) Diptera (Diptera) trypetid Superfamily
It (Tephritidae), is one of the big section in Diptera with Important Economic meaning, larva is potential food, colonizes in fruit
Endanger each portion of plant in entity, from root, stem, leaf, Hua Naizhi fruit, cause fruit rot, shedding, in addition can not harvest, to fruit
The yield of tree causes to seriously threaten.Trypetid class insect about 4200 known to the world is under the jurisdiction of 500 categories in kind.Wherein harmful trypetid
Type belongs to up to 15,22 subgenus, and more than 150, host range is wide, and only fruit plant just has 24 sections 42 category, and vegetable plants have 4
Section 16 belongs to.Fruit fly refer to it is some endanger fruit, vegetables and flowers and other crops, fruits and vegetables production and international trade etc. are constituted
The harmful trypetid type of worldwide or provincialism threatened.It is emphasis quarantine pair that many countries, which will endanger serious trypetid type column,
As, and stringent Quarantine control is carried out it.Currently, mainly using the anti-trypetid of chemical pesticide in agricultural production, but long-term a large amount of
Use chemical pesticide that trypetid is made to produce higher level to including multiclass medicaments such as organic phosphorus, pyrethroid and antibiotics
Drug resistance, and be in rapid increase trend.How scientific and reasonable medication, delay trypetid to develop drug resistance chemical pesticide to have become
An important content in current trypetid chemical prevention.
The toxicity test of insecticide is that a kind of quantity of toxicity indicates, i.e. toxic degree.Purpose is intended to know a certain
Kind of insecticide to the toxicity of a certain pest how, or compare several pesticides to the difference of the virulence degree of a certain insect
It not, is as the virulence size for measuring a kind of insecticide to certain insect.Insecticide to the toxicity test of trypetid class adult be with
Bactrocera tau and melonfly are test object, evaluate various insecticides to the virulence of Bactrocera tau and melonfly, measure insecticide
Have very to the toxicity of Bactrocera tau and melonfly for producing practical Bactrocera tau and melonfly prevention and treatment and physiological effect experiment
Important meaning.Therefore, in behavior of the research insecticide to Bactrocera tau and melonfly, physiological change and during influencing etc.,
It is frequently necessary to the toxicity test work for first carrying out insecticide to Bactrocera tau and melonfly.
Summary of the invention
The purpose of the present invention is to provide a kind of toxicity test device of trypetid adult stomach toxicity and toxicity test methods.This
Invention have clever structure, it is easy to use, low in cost, be remarkably improved labor efficiency reduce labor intensity, improve virulence survey
The advantages that fixed accuracy.
The present invention is carried out using following technical scheme.
A kind of toxicity test device of trypetid class adult stomach toxicity, the inventive system comprises toxicity test tanks and adult to collect
Bottle；Toxicity test tank includes the cover and tank body that top is arranged in, and ventilating mechanism A is provided in the middle part of cover；In tank base
It is provided with into worm channel and objective table；The bottleneck of adult receiving flask is connect by entering worm channel with toxicity test tank.In toxicity test tank
Settable there are two objective tables, to be put into food and moisture；The present invention is two parts composition, places reality to be measured in adult receiving flask
Fly adult, toxicity test tank are provided with objective table and can hold the adult forage mixture with medical fluid and immerse the degreasing of moisture content
Cotton provides food and moisture content, is drowned using absorbent cotton in order to which anti-trypetid adult falls into water, thereby may be ensured that the reliable of experiment
Property；The lid of the toxicity test upper end is to prevent confined space from making trypetid to guarantee that trypetid adult normally breathes
Adult death causes experimental error.It is to allow the trypetid in toxicity test tank smoothly to fly into virulence survey into worm channel that bottom end, which has,
Determine tank.Apparatus structure is toxicity test tank upper, adult receiving flask under, it is such can be designed so that trypetid adult smoothly from
It is advanced into toxicity test tank (trypetid class adult has habit winged upwards).Toxicity test tank and adult receiving flask of the invention,
It is transparent plastics material, facilitates the death rate of Observe and measure insect in toxicity test experiment and facilitate meter when collecting insect
Number.Toxicity test tank and adult receiving flask of the present invention can be dismantled, and be convenient for carrying, can be in measuring cell or outdoor
The virulence of trypetid adult, the cleaning problem after being also convenient to use.
In pest toxicity test, in a limited space in simulate the state of nature, this evil to the later period as much as possible
Authenticity, the science of worm poison power measurement result give high help, and the result with reference to toxicity test is foundation, kill for establishment
The parameters such as the use concentration of worm medicine, quantity, lethal events, which provide, accurate guarantees have the accurate guarantee that could make
Guaranteeing that effective kill to pest is outer when concrete application, the harm of environment is being accomplished to influence minimum.Therefore, the present invention sufficiently examines
Consider trypetid in a limited space in guarantee the smooth of air circulation as much as possible, greatly reduce since space is relatively closed and
The anoxic caused by pest influences.
The following contents, the present invention provide the structure of three kinds of ventilating mechanism A and the structure of two kinds of ventilating mechanism B.By simply to
Complexity, to realize the gas permeability of toxicity test device of the present invention.Position that ventilating mechanism A and ventilating mechanism B is arranged is different, can be with
It is combined with each other to form different products.
It is further that the structure of ventilating mechanism A of the present invention is that a big venthole is provided on cover, logical
Gas-permeable nets are set on stomata, and the mesh number of gas-permeable nets is 80~100 mesh.Since the individual of trypetid adult is larger, so if selection mesh
The gas-permeable nets that number is greater than 100 mesh can then make the airtight event that causes death of trypetid adult if mesh number is smaller be to lead to trypetid
Escape, causes error to experiment.
Apparatus of the present invention Reference Design specification is the size of the toxicity test tank: tank body high 5.5cm, diameter 10cm
Transparent plastic can, top cover diameter be 10cm, gas-permeable nets diameter 6.5cm, mesh number be 100 mesh；Objective table diameter is
3.5cm, a height of 1cm；Upper shield diameter 3cm high 2cm；Adult receiving flask bottleneck diameter 3.6cm, bottle height 8cm.
It is further that the structure of ventilating mechanism A of the present invention is that M small ventholes are uniformly provided on cover
A, and the aperture of small venthole A is 1mm~1.5mm.The aperture of small venthole A is less than the body diameter of pest to be measured (trypetid), no
It can be by being drilled out in small venthole A.
It is further that the structure of ventilating mechanism A of the present invention is to open up circular groove on cover top, round recessed
It is embedded a round turntable on slot, is uniformly provided with mutually matched N pairs up and down simultaneously on circular groove bottom and round turntable
Small venthole B；When round turntable rotation, the small venthole B of small venthole B and circular groove bottom on round turntable are formed
Dislocation, to control the opening and closing of small venthole B and open the size of size.Since the aperture of its small venthole B can be with
Changed by rotation, therefore the aperture of small venthole B can be set to the body diameter more than or equal to pest to be measured (trypetid).To
It,, will be small when losing flight performance when dead in the vigor that gradually losing after surveying pest (trypetid) feeding infectious agent
Venthole B is fully open, so that the air flowing in toxicity test tank is almost without obstruction, closer to natural air circulation
State.
It is further that the present invention is provided with ventilating mechanism B on tank wall；The structure of ventilating mechanism B is, in tank body
M small venthole A are uniformly provided on side wall, and the aperture of small venthole A is 1mm~1.5mm.
It is further that the present invention is provided with ventilating mechanism B on tank wall；The structure of ventilating mechanism B is, in tank body
Top and bottom be provided with the ring set of slightly above tank wall, i.e. upper ring cap and lower ring set；Between upper ring cap and lower ring set
It is provided with the annular outer wall that diameter is slightly larger than tank diameter；Annular outer wall and tank body rotation connect；In annular outer wall and tank
Mutually matched S is to small venthole C above and below being uniformly provided with simultaneously on body sidewall；When annular outer wall rotation, on annular outer wall
Small venthole C and tank body on small venthole C formed dislocation, to control opening and closing and the opening ruler of small venthole C
Very little size.The ventilating mechanism B of this structure increases this component of annular outer wall newly, makes small venthole C's by rotating the component
Opening and closing, the aperture of small venthole C can be set to the body diameter more than or equal to pest to be measured (trypetid), and usage is the same as aforementioned small venthole
The usage of B.
The Jin Chongjin worm implementation of toxicity test pot bottom of the present invention is designed as three kinds.The first and for it is aforementioned be only into
Adult receiving flask bottleneck is inserted into worm channel and leaves it in toxicity test tank and adult receiving flask two spaces by the mode of worm channel
The defect of interior flight, such mode is 1) pest (trypetid) to be measured is simultaneously in toxicity test tank and the empty faces of adult receiving flask two
When interior activity, some can be rested in adult receiving flask side wall certainly, artificially reduce the chance of its feeding food；2)
After pest to be measured (trypetid) feeding food, in state that is weak, being at death's door of being poisoned, but some trypetids are because of existence
The reason of instinct still will continue to trend, but since it is flown or slid to adult receiving flask in activity, and without extra energy
When power is flown to by food, death can be led to because of starvation, to will affect pest to be measured (trypetid) completely because of the toxic food of feeding
Object and death final experimental data, the experiment Exact Travelling in later period is had some impact on.
It is further that the present invention is set as in the worm channel that enters of toxicity test pot bottom into worm mechanism, which is that will enter worm
The middle part of toxicity test pot bottom is arranged in hole；Upper shield is set on adult receiving flask bottleneck, and the diameter of upper shield is slightly less than into worm
Bore dia, set tops offer several adults and pierce hole.The setting of upper shield can reduce what drawbacks described above 1 and defect 2 occurred
Technical solution is increased to certain altitude to a certain extent by probability.
It is further that the present invention is set as in the worm channel that enters of toxicity test pot bottom into worm mechanism, which is that will enter worm
The position that toxicity test pot bottom leans on proximal edge is arranged in hole；Round tray is set under toxicity test pot bottom, is surveyed in virulence
Determine to cooperate simultaneously on pot bottom and round tray and open up into worm channel；The inner wall of round tray is set on the outer wall of lower ring set therewith
Rotation connection；When round tray rotation, on round tray enter worm channel and the worm channel that enters of toxicity test pot bottom forms dislocation,
Realize the opening and closing into worm channel.The setting of round tray has prevented the case where aforementioned drawback 1 and defect 2 completely, makes completely
Pest (trypetid) to be measured is only movable in toxicity test tank, and the case where later period simulates after the probability of feeding poisonous food and feeding
It is very close with nature, therefore the experimental result measured is also more acurrate and scientific.
Use the toxicity test method of apparatus of the present invention, which is characterized in that method includes the following steps:
Step 1 prepares 1 group of totally 6 toxicity test device, separation determination tank and receiving flask；Collect emergence 2-3 days realities
For fly adult as trypetid to be measured, trypetid adult to be measured needs to carry out 24 hours Nature enemies, improves feeding rate；
Step 2 is put into receiving flask for examination trypetid adult for 10；Then insecticide is configured to 5 kinds of various concentration gradients
Medical fluid, check experiment then use clear water, for use；
Step 3 mixes trypetid adult forage with the reagent agent of 5 various concentration gradients, and blank control uses clear water
It is mixed with adult forage, 0.3ml medical fluid or water is added in 3g adult forage, dries, and mixed feed is transferred to virulence by transfer later
It measures in the objective table in tank, places the absorbent cotton after being flooded in each toxicity test tank in another objective table, mend
Water-filling part；The trypetid to be measured that adult receiving flask is collected into is connected in toxicity test tank by entering worm channel, direction is virulence survey
Tank is determined upper, and adult receiving flask voluntarily flies into toxicity test tank in lower setting, the habit flown upwards by trypetid；
Step 4 rotates round tray, closes into worm channel after trypetid to be measured flies into toxicity test tank completely；
6 toxicity test devices are all placed in growth cabinet by step 5, growth cabinet condition be temperature 24~
26 DEG C, humidity is 55%~60%, and periodicity of illumination L:D=14:10,24 as a child observed and recorded trypetid adult survival condition；
Step 6 slowly rotates round turntable, is gradually opened small venthole B with the gradually death of trypetid to be measured, until from
Finally fully open；Slowly rotary annular outer wall simultaneously, is gradually opened small venthole C, until from finally fully opening；
Step 7 carries out Data Management Analysis using SPSS22.0, obtains virulence regression equation and lethal concentration of 50 LC50.
The invention has the benefit that the above structure that the device of trypetid class adult toxicity test of the present invention uses, is used for
Toxicity test of the various insecticides to trypetid class adult.Experimental provision is divided into two parts, facilitates experimental implementation, and it is accurate to increase experiment
Property；Toxicity test tank is wide cylindrical structure, in order to have broader space to make trypetid movable and hold food and water, with air defense
Between too small cause death；Gas-permeable nets on toxicity test pipe upper end lid be in order to keep during the experiment oxygen into
Enter, prevents from lacking oxygen and cause trypetid adult dead；Adult receiving flask is connected by porose lid with toxicity test pipe, is prevented
It only escapes when shifting worm to be measured, causing need not workload.It is the configuration of the present invention is simple, convenient to use, low in cost, clever
Active accuracy that is high, being remarkably improved labor efficiency reduction labor intensity, raising toxicity test.Method of the invention has wound
New property, and easy to operate, processing is easy, the period is relatively short, accuracy is accurate and detection efficiency is high.Operator easily grasps, special
The toxicity test to a variety of insecticides to trypetid class adult is not easy for also may be used even if operator does not have relevant professional knowledge
Learn in a short time.Measuring method of the invention can be suitble to be widely popularized in major plant protection unit, can be China's pesticide
The development of cause and the quantitative effective control of pesticide bring significant directiveness effect.
The present invention is further explained with reference to the accompanying drawings and detailed description.
Detailed description of the invention
Fig. 1 is the general assembly schematic diagram of one of structure of the present invention.
Fig. 2 is the can cover structure schematic diagram of another structure of the present invention.
Fig. 3 is the general assembly schematic diagram of another structure of the present invention.
Fig. 4 is the assembly structure diagram of Fig. 3 structure.
Fig. 5 is one of structural schematic diagram into worm mechanism cooperated with Fig. 3 structure.
Fig. 6 is the general assembly schematic diagram of another structure of the present invention.
Fig. 7 is the assembly structure diagram of Fig. 6 structure.
Fig. 8 is another structural schematic diagram into worm mechanism cooperated with Fig. 6 structure.
Figure label are as follows: toxicity test tank (1), cover (3), tank body (4), objective table (5), leads to greatly adult receiving flask (2)
Stomata (6), gas-permeable nets (7), small venthole A (8), circular groove (9), round turntable (10), small venthole B (11), upper ring cap
(12), lower ring set (13), annular outer wall (14), small venthole C (15), enter worm channel (16), upper shield (17), adult and pierce hole
(18), round tray (19).
Specific embodiment
In order to make those skilled in the art, methods of this invention will be better understood, combined with specific embodiments below to this hair
It is bright to be described in further detail.
A kind of toxicity test device of trypetid class adult stomach toxicity, the inventive system comprises toxicity test tanks 1 and adult to receive
Collect bottle 2；Toxicity test tank 1 includes the cover 3 and tank body 4 that top is arranged in, and ventilating mechanism A is provided in the middle part of cover 3；In tank
4 bottom of body is provided with into worm channel 16 and objective table 5；The bottleneck of adult receiving flask 2 is connected by entering worm channel 16 and toxicity test tank 1
It connects.Settable in toxicity test tank 1 there are two objective tables 5, to be put into food and moisture；The present invention is two parts composition, adult
Place trypetid adult to be measured in receiving flask 2, toxicity test tank 1 is provided with objective table 5, and can to hold the adult forage with medical fluid mixed
It closes object and immerses the absorbent cotton of moisture content, food and moisture content are provided, be drowned using absorbent cotton in order to which anti-trypetid adult falls into water, from
And it can guarantee the reliability of experiment；The lid on 1 top of toxicity test tank is to guarantee that trypetid adult normally exhales
It inhales, prevents confined space from trypetid adult death being made to cause experimental error.Bottom end has into worm channel 16, is to allow toxicity test tank 1
In trypetid smoothly fly into toxicity test tank 1.Apparatus structure is toxicity test tank 1 upper, and adult receiving flask 2 is under, in this way
Can be designed so that trypetid adult smoothly from being advanced into toxicity test tank 1 (trypetid class adult has habit winged upwards).This
The toxicity test tank 1 and adult receiving flask 2 of invention, are transparent plastics material, the convenient Observe and measure in toxicity test experiment
The death rate of insect and facilitate collect insect when counting.Toxicity test tank 1 and adult receiving flask 2 of the present invention can be torn open
It opens, is convenient for carrying, it can be with the cleaning problem in measuring cell or the virulence of outdoor trypetid adult, after being also convenient to use.(figure
1)
In pest toxicity test, in a limited space in simulate the state of nature, this evil to the later period as much as possible
Authenticity, the science of worm poison power measurement result give high help, and the result with reference to toxicity test is foundation, kill to establish
The parameters such as the use concentration of worm medicine, quantity, lethal events, which provide, accurate guarantees have the accurate guarantee that could make
Guaranteeing that effective kill to pest is outer when concrete application, minimum influence is accomplished to the harm of environment.Therefore, the present invention sufficiently examines
Consider trypetid in a limited space in guarantee the smooth of air circulation as much as possible, greatly reduce since space is relatively closed and
Anoxic is caused to influence pest.
The following contents, the present invention provide the structure of three kinds of ventilating mechanism A and the structure of ventilating mechanism B.From simple to complex,
To realize the gas permeability of toxicity test device of the present invention.The position that ventilating mechanism A and ventilating mechanism B is arranged is different, can be mutual
Combination is to form different products.
It is further that the structure of ventilating mechanism A of the present invention is that a big venthole 6 is provided on cover 3,
Gas-permeable nets 7 are set on venthole, and the mesh number of gas-permeable nets 7 is 80~100 mesh.Since the individual of trypetid adult is larger, so if choosing
Selecting mesh number can then make the airtight event that causes death of trypetid adult if mesh number is smaller be to lead greater than the gas-permeable nets 7 of 100 mesh
Trypetid escape is caused, error is caused to experiment.(Fig. 1)
Apparatus of the present invention Reference Design specification is the size of the toxicity test tank 1: 5.5cm high, the diameter of tank body 4
The transparent plastic can of 10cm, top cover diameter are 10cm, and 7 diameter 6.5cm of gas-permeable nets, mesh number is 100 mesh；5 diameter of objective table
For 3.5cm, a height of 1cm；17 diameter 3cm high 2cm of upper shield；Adult receiving flask 2 bottleneck diameter 3.6cm, bottle height 8cm.
It is further that the structure of ventilating mechanism A of the present invention is that M small ventholes are uniformly provided on cover 3
A8, and the aperture of small venthole A8 is 1mm~1.5mm.The aperture of small venthole A8 is less than the body diameter of pest to be measured (trypetid),
It cannot be by being drilled out in small venthole A8.(Fig. 2)
It is further that the structure of ventilating mechanism A of the present invention is to open up circular groove 9 on 3 top of cover, it is round
It is embedded a round turntable 10 on groove 9, is uniformly provided with phase interworking up and down simultaneously on 9 bottom of circular groove and round turntable 10
The N of conjunction is to small venthole B 11；Small venthole B 11 and circular groove 9 when round turntable 10 rotates, on round turntable 10
The small venthole B 11 of bottom forms dislocation, to control the opening and closing of small venthole B 11 and open the size of size.
Since the aperture of its small venthole B 11 can be changed by rotation, the aperture of small venthole B 11 be can be set to greatly
In the body diameter for being equal to pest to be measured (trypetid).After pest to be measured (trypetid) feeding infectious agent, in gradually losing work
It is when losing flight performance, small venthole B 11 is fully open when power is dead, so that the air stream in toxicity test tank 1
It moves as almost without obstruction, the state of closer natural air circulation.(Fig. 3)
It is further that the present invention is provided with ventilating mechanism B on 4 side wall of tank body；The structure of ventilating mechanism B is, in tank
M small venthole A8 are uniformly provided on 4 side wall of body, and the aperture of small venthole A8 is 1mm~1.5mm.(there is not attached drawing table
Show)
It is further that the present invention is provided with ventilating mechanism B on 4 side wall of tank body；The structure of ventilating mechanism B is, in tank
The top and bottom of body 4 are provided with the ring set of slightly above 4 side wall of tank body, i.e. upper ring cap 12 and lower ring set 13；In 12 He of upper ring cap
The annular outer wall 14 that diameter is slightly larger than 4 diameter of tank body is provided between lower ring set 13；Annular outer wall 14 rotates the company of contact with tank body 4
It connects；Mutually matched S is to small venthole C 15 above and below being uniformly provided with simultaneously on annular outer wall 14 and 4 side wall of tank body；Work as ring
When shape outer wall 14 rotates, the small venthole C 15 on small venthole C 15 and tank body 4 on annular outer wall 14 forms dislocation, thus
It controls the opening and closing of small venthole C 15 and opens the size of size.The ventilating mechanism B of this structure increases annular outer wall 14 newly
This component, the opening and closing of small venthole C 15 is made by rotating the component, and the aperture of small venthole C 15 can be set to greatly
In the body diameter for being equal to pest to be measured (trypetid), usage of the usage with aforementioned small venthole B 11.(Fig. 4 or Fig. 7)
The Jin Chongjin worm implementation of 1 bottom of toxicity test tank of the present invention is designed as three kinds.The first and for it is aforementioned be only
2 bottleneck of adult receiving flask is inserted into worm channel 16 and leaves it in toxicity test tank 1 and adult receiving flask by the mode for entering worm channel 16
Flight in 2 two spaces, the defect of such mode is 1) pest (trypetid) to be measured collects in toxicity test tank 1 and adult simultaneously
In 2 two, bottle empty faces when activity, some can be rested in 2 side wall of adult receiving flask certainly, artificially reduce its feeding
The chance of food；2) it after pest to be measured (trypetid) feeding food, is being in the state that poisoning is weak, is at death's door, but
Some trypetids are because the reason of life instinct still will continue to trend, but since it is flown or slid in activity to adult receiving flask
In 2, and when flying without excess capacity to by food, death can be led to because of starvation, so that it is complete to will affect pest to be measured (trypetid)
Final experimental data dead due to feeding poisonous food entirely, has some impact on the experiment Exact Travelling in later period.
It is further that enter worm channel 16 of the present invention in 1 bottom of toxicity test tank is set as into worm mechanism, which is to incite somebody to action
Enter the middle part that 1 bottom of toxicity test tank is arranged in worm channel 16；On 2 bottleneck of adult receiving flask be arranged upper shield 17, upper shield 17 it is straight
Diameter is slightly less than into 16 diameter of worm channel, offers several adults at the top of upper shield 17 and pierces hole 18.The setting of upper shield 17 can be reduced
The probability that defect 1 and defect 2 occur is stated, technical solution is increased to certain altitude to a certain extent.(Fig. 3, Fig. 4, Fig. 5)
It is further that enter worm channel 16 of the present invention in 1 bottom of toxicity test tank is set as into worm mechanism, which is to incite somebody to action
Enter worm channel 16 and the position that proximal edge is leaned in 1 bottom of toxicity test tank is set；Round tray is set under 1 bottom of toxicity test tank
19, cooperation is opened up into worm channel 16 simultaneously on 1 bottom of toxicity test tank and round tray 19；The inner wall of round tray 19 is set in
It is rotationally connected on the outer wall of lower ring set 13；When round tray 19 rotates, enter worm channel 16 and virulence on round tray 19
The worm channel 16 that enters of measurement 1 bottom of tank forms dislocation, realizes the opening and closing into worm channel 16.The setting of round tray 19 is shut out completely
Aforementioned drawback 1 and the case where defect 2 absolutely, makes pest to be measured (trypetid) only movable in toxicity test tank 1, feeding has completely
Simulation is very close with nature the case where the later period after the probability of malicious food and feeding, therefore the experimental result measured is also more quasi-
It is really and scientific.(Fig. 6, Fig. 7, Fig. 8)
Use the toxicity test method of apparatus of the present invention, which is characterized in that method includes the following steps:
Step 1 prepares 1 group of totally 6 toxicity test device, separation determination tank and receiving flask；Collect emergence 2-3 days realities
For fly adult as trypetid to be measured, trypetid adult to be measured needs to carry out 24 hours Nature enemies, improves feeding rate；
Step 2 is put into receiving flask for examination trypetid adult for 10；Then insecticide is configured to 5 kinds of various concentration gradients
Medical fluid, check experiment then use clear water, for use；
Step 3 mixes trypetid adult forage with the reagent agent of 5 various concentration gradients, and blank control uses clear water
It is mixed with adult forage, 0.3ml medical fluid or water is added in 3g adult forage, dries, and mixed feed is transferred to virulence by transfer later
It measures in the objective table 5 in tank 1, places the degreasing after being flooded in each toxicity test tank 1 in another objective table 5
Cotton supplements moisture content；The trypetid to be measured that adult receiving flask 2 is collected into is connected in toxicity test tank 1 by entering worm channel 16, direction
It is toxicity test tank 1 upper, adult receiving flask 2 voluntarily flies into toxicity test tank in lower setting, the habit flown upwards by trypetid
1；
Step 4 rotates round tray 19, closes into worm channel 16 after trypetid to be measured flies into toxicity test tank 1 completely；
6 toxicity test devices are all placed in growth cabinet by step 5, growth cabinet condition be temperature 24~
26 DEG C, humidity is 55%~60%, and periodicity of illumination L:D=14:10,24 as a child observed and recorded trypetid adult survival condition；
Step 6 slowly rotates round turntable 10, is gradually opened small venthole B with the gradually death of trypetid to be measured
11, until from finally fully opening；Slowly rotary annular outer wall 14 simultaneously, is gradually opened small venthole C 15, until from last complete
It opens；
Step 7 carries out Data Management Analysis using SPSS22.0, obtains virulence regression equation and lethal concentration of 50 LC50.
The specific operation process tested using structure of the invention
(1) experiment condition:
Experiment carries out in growth cabinet, and growth cabinet (rising instrument, LTC-1000 in Shanghai three) condition setting is as follows:
Temperature is 24 DEG C, periodicity of illumination L:D=14:10, and raising humidity is 60%；
(2) selected insect source:
It is adopted in Yunnan Province Mengzi city decayed fruit for the Bactrocera tau and melonfly worm sources of examination, decayed fruit is taken back laboratory and is waited
Trypetid jumps out on ready sand and pupates in decayed fruit, and emergence is raised after obtaining adult using man-made feeds.Laboratory
Bactrocera tau and melonfly raising alreadyd exceed for 50 generations.The adult of the Bactrocera tau and melonfly sprouted wings 2~3 days is collected before experiment
It is for experiment.And handle within hungry 24 hours, improve feeding rate.
(3) insecticide is configured that
First insecticide is measured and is dissolved in the medical fluid for being configured to larger concentration in clear water after conversion weighs, is then diluted
Medical fluid to 5 constant gradient concentration is contained in culture dish；
(4) Bactrocera tau and melonfly are encased in adult by 10 Bactrocera taus of each corollary apparatus and melonfly adult
It in receiving flask, is connect by porose lid with toxicity test pipe, so that trypetid adult is flown into toxicity test tank and carry out feeding；
(5) it is placed on after being raised 24 hours in growth cabinet and observes survival condition；
(6) morphological feature of Bactrocera tau:
Adult Individual Size is widely different, long (6.61 ± 0.42) mm of male fly body, long (8.56 ± 1.16) mm of female fly body；Wing
Open up (7.30~8.60) mm；Body colour yellowish-brown is to bronzing, the long 6~9mm of the body that is averaged；Head yellow, face spot black, 2 pieces, in
Deng big；Overhead has a short red grouper；There are 1 pair of bristle in volume brown, volume upside, have 3 pairs of bristles on the downside of volume, few has 2 pairs；Mesothorax
Backboard yellowish-brown, tool seam, rear side have middle yellow item 3, and the yellow item of two sides is symmetrical after seam, finally after rear supraalar bristle；Chest
There is apparent black speckle between omoplate and transverse joint and after seam between vitta；Escutcheon yellow has a narrow fast black base band, tool 2
To pelta end hair on the neck；Wing grows 5.7~8.5mm, protoloma infuscate；Sufficient leg section yellow, forward and backward foot tibia brown, mesopodium tibia are light
Brown；Abdomen yellowish-brown, Section 2 backboard have brown item, and Section 3 backboard leading edge has crineous cross band, and the 4th, 5 section lateral margins respectively have one
The spot of dark sepia, Section of 35 backboard have taeniae among black, this taeniae is interrupted by internode sometimes.
(7) morphological feature of melonfly:
The bodily form is like bee, and yellowish-brown to bronzing, long 7~9mm, wide 3~4mm, the long 7mm of wing, female adult is more smaller than male worm, first plumage
The adult body colour of change is thin, 50% of size not as good as oviposition adult.Respectively there are 1 yellow spotting in shirtfront back side two sides, and mesothorax two sides are each
There is 1 thicker yellow vertical bar spot, the back side there are 3 yellow longitudinal grins arranged side by side；Parachute matter, transparent, glossy, auxilairs and stern area are each
There is 1 long streak, the apex of the wing has 1 round spot, and ordinary crossvein and mediocubital crossvein have beloid patch.
(8) toxicity test of the insecticide to Bactrocera tau
1 multiple killing teichomycin of table (Spinosad) handles data record to Bactrocera tau adult for 24 hours
Multiple killing teichomycin processing Bactrocera tau high concentration effect is obvious, 3-5mg.L-1Inside the death rate is above 50% for 24 hours,
24hLC50For 3.064mg.L-1, it compares the death rate and is consistently lower than 5%, credible result.
2 multiple killing teichomycin of table (Spinosad) handles data record to melonfly adult for 24 hours
Multiple killing teichomycin can be obtained to the effect of melonfly high concentration stomach toxicity by table 2, for 24 hours LC50 3.546mg.L-1, 5-
9mg.L-1The death rate is above 50%, and the control death rate is consistently lower than 5%, credible result.
Toxicity test result of 3 multiple killing teichomycin of table to Bactrocera tau and melonfly
Table 3 the result shows that, using stomach toxicity method measurement multiple killing teichomycin to Bactrocera tau and melonfly adult toxicity test result
In, the stomach toxicity effect to melonfly, the LC of virulence are better than to the stomach toxicity effect of Bactrocera tau50Value is respectively 3.064 Hes
3.546, related coefficient is respectively 0.842 and 0.863.Show that Bactrocera tau and melonfly theoretical death rate and actual are aobvious
It writes and coincide, gained virulence regression equation preferably reflects multiple killing teichomycin to the indoor virulence of Bactrocera tau and melonfly.
The adult toxicity test process and result of Bactrocera tau and melonfly are described in detail above, but utilized
Method of the invention can not only carry out toxicity test to the adult of Bactrocera tau and melonfly, also can equally to other trypetids at
Worm carries out toxicity test, and the toxicity test result of different trypetid adults is obtained in the case where most efficiently；And to shaped like trypetid
The such insect of class adult can also carry out the toxicity test of adult using the invention method.
As extension, although title of the invention is recorded as the toxicity test device of trypetid class adult stomach toxicity, not only
Trypetid class adult is used, and is had with other approximate insects (pest) of trypetid class adult as long as being suitble to technical solution of the present invention
Structure or method, belong to the extension of technical solution of the present invention.
Although above the present invention is described in detail with a general description of the specific embodiments,
On the basis of the present invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Cause
This, these modifications or improvements should belong to unless playing significant innovation on the basis of small deviation spirit of that invention
The scope of protection of present invention.
Claims (10)
1. a kind of toxicity test device of trypetid class adult stomach toxicity, which is characterized in that including toxicity test tank and adult receiving flask；
Toxicity test tank includes the cover and tank body that top is arranged in, and ventilating mechanism A is provided in the middle part of cover；It is arranged in tank base
Have into worm channel and objective table；The bottleneck of adult receiving flask is connect by entering worm channel with toxicity test tank.
2. a kind of toxicity test device of trypetid class adult stomach toxicity according to claim 1, which is characterized in that described is saturating
The structure of mechanism of qi structure A is that a big venthole is provided on cover, and gas-permeable nets are arranged on venthole, and the mesh number of gas-permeable nets is
80~100 mesh.
3. a kind of toxicity test device of trypetid class adult stomach toxicity according to claim 1, which is characterized in that described is saturating
The structure of mechanism of qi structure A is M small venthole A to be uniformly provided on cover, and the aperture of small venthole A is 1mm~1.5mm.
4. a kind of toxicity test device of trypetid class adult stomach toxicity according to claim 1, which is characterized in that described is saturating
The structure of mechanism of qi structure A is to open up circular groove on cover top, a round turntable is embedded on circular groove, at circular groove bottom
Mutually matched N is to small venthole B above and below being uniformly provided with simultaneously in portion and round turntable；It is round when round turntable rotation
The small venthole B of small venthole B and circular groove bottom on turntable forms dislocation, thus control small venthole B unlatching and
Close and open the size of size.
5. a kind of toxicity test device of trypetid class adult stomach toxicity according to Claims 2 or 3 or 4, which is characterized in that
Ventilating mechanism B is provided on tank wall；The structure of ventilating mechanism B is that M small ventilations are uniformly provided on tank wall
Hole A, and the aperture of small venthole A is 1mm~1.5mm.
6. a kind of toxicity test device of trypetid class adult stomach toxicity according to Claims 2 or 3 or 4, which is characterized in that
Ventilating mechanism B is provided on tank wall；The structure of ventilating mechanism B is to be provided with slightly above in the top and bottom of tank body
The ring set of tank wall, i.e. upper ring cap and lower ring set；Diameter is provided between upper ring cap and lower ring set slightly larger than tank diameter
Annular outer wall；Annular outer wall and tank body rotation connect；It is uniformly provided with simultaneously on annular outer wall and tank wall
Under mutually matched S to small venthole C；It is small logical on the small venthole C and tank body on annular outer wall when annular outer wall rotation
Stomata C forms dislocation, to control the opening and closing of small venthole C and open the size of size.
7. a kind of toxicity test device of trypetid class adult stomach toxicity according to claim 5, which is characterized in that surveyed in virulence
The worm channel that enters for determining pot bottom is set as into worm mechanism, which is, the middle part that will enter worm channel toxicity test pot bottom is arranged in；?
Upper shield is set on adult receiving flask bottleneck, and the diameter of upper shield is slightly less than into worm channel diameter, covers tops and offers several adults brills
Enter hole.
8. a kind of toxicity test device of trypetid class adult stomach toxicity according to claim 6, which is characterized in that surveyed in virulence
The worm channel that enters for determining pot bottom is set as into worm mechanism, which is, the middle part that will enter worm channel toxicity test pot bottom is arranged in；?
Upper shield is set on adult receiving flask bottleneck, and the diameter of upper shield is slightly less than into worm channel diameter, covers tops and offers several adults brills
Enter hole.
9. a kind of toxicity test device of trypetid class adult stomach toxicity according to claim 6, which is characterized in that surveyed in virulence
The worm channel that enters for determining pot bottom is set as into worm mechanism, which is that will enter worm channel setting in toxicity test pot bottom by proximal edge
Position；Round tray is set under toxicity test pot bottom, toxicity test pot bottom on round tray simultaneously with running jointly
If entering worm channel；The inner wall of round tray is set in rotationally connected on the outer wall of lower ring set；It is round when round tray rotation
The worm channel that enters for entering worm channel and toxicity test pot bottom on pallet forms dislocation, realizes the opening and closing into worm channel.
10. using the toxicity test method of device as claimed in claim 9, which is characterized in that method includes the following steps:
Step 1 prepares 1 group of totally 6 toxicity test device, separation determination tank and receiving flask；Collect emergence 2-3 days trypetid at
For worm as trypetid to be measured, trypetid adult to be measured needs to carry out 24 hours Nature enemies, improves feeding rate；
Step 2 is put into receiving flask for examination trypetid adult for 10；Then insecticide is configured to 5 kinds of various concentration gradient medicines
Liquid, check experiment then use clear water, for use；
Step 3 mixes trypetid adult forage with the reagent agent of 5 various concentration gradients, blank control using clear water at
The mixing of worm feed, 3g adult forage are added 0.3ml medical fluid or water, dry, and mixed feed is transferred to toxicity test by transfer later
In objective table in tank, the absorbent cotton after being flooded is placed in each toxicity test tank in another objective table, supplements water
Part；The trypetid to be measured that adult receiving flask is collected into is connected in toxicity test tank by entering worm channel, direction is toxicity test tank
Upper, adult receiving flask voluntarily flies into toxicity test tank in lower setting, the habit flown upwards by trypetid；
Step 4 rotates round tray, closes into worm channel after trypetid to be measured flies into toxicity test tank completely；
6 toxicity test devices are all placed in growth cabinet by step 5, and growth cabinet condition is temperature 24~26
DEG C, humidity is 55%~60%, and periodicity of illumination L:D=14:10,24 as a child observed and recorded trypetid adult survival condition；
Step 6 slowly rotates round turntable, is gradually opened small venthole B with the gradually death of trypetid to be measured, until from last
It fully opens；Slowly rotary annular outer wall simultaneously, is gradually opened small venthole C, until from finally fully opening；
Step 7 carries out Data Management Analysis using SPSS22.0, obtains virulence regression equation and lethal concentration of 50 LC50.
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| CN111543392A (en) * | 2020-04-27 | 2020-08-18 | 浙江大学 | Method and device for large-scale raising of fruit fly parasitic wasps |
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