CN216601153U - Indoor bioassay device for cowpea pest clover leaf liriomyza sativae - Google Patents
Indoor bioassay device for cowpea pest clover leaf liriomyza sativae Download PDFInfo
- Publication number
- CN216601153U CN216601153U CN202123330542.XU CN202123330542U CN216601153U CN 216601153 U CN216601153 U CN 216601153U CN 202123330542 U CN202123330542 U CN 202123330542U CN 216601153 U CN216601153 U CN 216601153U
- Authority
- CN
- China
- Prior art keywords
- water culture
- cup cover
- culture cup
- cup
- indoor bioassay
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a cowpea pest clover leaf liriomyza indoor bioassay device which comprises a water culture cup (1), a water culture cup cover (2), a cup cover (3) and a preservative film (4). The water culture cup (1) is provided with a water culture cup cover (2), a ventilation opening (2-1) is formed in the water culture cup cover, a cup cover cap (3) is bonded to the water culture cup cover (1), and the cup cover cap (3) is of a net structure; the preservative film (4) covers the cup mouth of the water culture cup (1). The utility model has simple structure, flexible use, convenient moving and cleaning and repeated use; the defects that the traditional indoor bioassay device for the liriomyza trifoliata is incapable of recycling, air is not circulated, humidity is too low and experimental data are influenced due to improper operation are overcome, and the sustainability and the simplicity of operation of the indoor bioassay device for the pests are improved.
Description
Technical Field
The utility model relates to a correlation technique in the field of indoor bioassay of clover leaf liriomyza, in particular to an indoor bioassay device of cowpea pest clover leaf liriomyza.
Background
The Liriomyza trifolii (Burgess) belongs to Diptera, Dioscoreaceae and Drosophila, is a worldwide vegetable pest and is widely distributed around the world; it is highly adaptable and breeds rapidly, has spread in temperate and tropical regions and is becoming more and more difficult to control. The larva stage and the adult stage of the liriomyza trifoliata can damage crops, the main host plant is cowpea, and an ovipositor of the adult plants stabs holes on leaves to lay eggs and forms silver spots on the surfaces of the plant leaves. After the eggs are hatched into larvae, the eggs are taken to eat the leaf blades and mesophyll tissues to form irregular snake-shaped white galleries. Not only can damage mesophyll tissues and chlorophyll and reduce photosynthesis of leaves, but also can lead the cowpea leaves to wither and fall off when serious, and plants die, thus causing huge loss to agricultural and horticultural crops. At present, the control of the liriomyza trifoliata still mainly adopts chemical control, but the drug resistance of the liriomyza trifoliata is rapidly developed, and a higher resistance level is generated to common chemical pesticides.
The pest drug resistance monitoring is an important basis for pest control, and the biological assay is an important technical means for pest drug resistance level monitoring, can reflect the sensitivity level of pests to chemical pesticides, and has guiding significance for field pesticide application and control thereof.
Traditional clover latent fly's indoor bioassay adopts culture dish and filter paper to go on mostly, soaks filter paper and spreads in the culture dish bottom, gives the leaf moisture, and the humidity of the bioassay environment can be guaranteed to this kind of device, but its unable assurance device circulation of air can influence the concentration of pesticide moreover when supplementing moisture for the leaf, finally influences the accuracy of living survey data.
SUMMERY OF THE UTILITY MODEL
The utility model aims to provide an indoor bioassay device for Liriomyza trifoliata to solve the problems in the background technology.
In order to achieve the purpose, the utility model provides the following technical scheme:
a cowpea pest clover leaf liriomyza indoor bioassay device comprises a water culture cup, a water culture cup cover, a cup cover cap and a preservative film. The water culture cup is provided with a water culture cup cover, the top end surface of the water culture cup cover is provided with a ventilation opening, a cup cover cap is bonded on the ventilation opening, and the cup cover cap is of a net structure; the preservative film is covered on the cup mouth of the water culture cup, and holes are formed according to needs when the preservative film is used.
As a further scheme of the utility model: the water culture cup and the water culture cup cover are made of transparent plastic materials, the specification and the size of the water culture cup and the water culture cup cover are not fixed (160 ml or 200ml can be selected), or the water culture cup and the water culture cup cover are fixed according to the number of placed cowpea seedlings, personal preference and experiment repetition times, and during selection, the caliber of the water culture cup cover is larger than that of the water culture cup.
As a further scheme of the utility model: the cup cover cap is made of a 200-mesh nylon gauze.
Compared with the existing indoor bioassay device for the liriomyza trifoliata, the indoor bioassay device has the beneficial effects that:
the utility model has relatively simple structure and reasonable design, and the device is light and easy to carry; meanwhile, the cleaning is convenient, and the cleaning can be repeatedly utilized; the specification and size of the used water culture cup and the water culture cup cover can be freely selected according to the requirements and preferences of the technical personnel for living test, and the water culture cup cover are made of transparent plastic materials, so that the state and experimental data of the liriomyza trifoliata can be observed and recorded at any time from the outside; the use of the preservative film is not only beneficial to the survival of the cowpeas, but also can collect the pupa of the liriomyza trifoliata and prevent the pupa from falling into water; the arranged ventilation and air-permeable opening is simple and practical and is beneficial to air circulation in the device; the cup cover with the nylon gauze of 200 meshes is light and breathable, and can prevent and control liriomyza trifoliate from flying out of the bottle; the utility model eliminates the defects that the traditional indoor bioassay device for the liriomyza trifoliata is incapable of recycling, air is not circulated, the humidity is too low and experimental data is influenced by improper operation, and improves the sustainability and the simplicity of operation of the indoor bioassay device for the pests.
Drawings
FIG. 1 is a schematic view showing the overall structure of an indoor bioassay device for Liriomyza trifoliata;
FIG. 2 is a plan view of a water culture cup cover 2 in an indoor bioassay system for Liriomyza trifoliata.
Wherein: a water culture cup 1; a water culture cup cover 2; 2-1 of ventilation openings; a cup cover 3; and (4) a preservative film.
Detailed Description
Hereinafter, the indoor bioassay system according to the embodiment of the present invention will be described in detail and fully with reference to the accompanying drawings, and it is to be understood that the described embodiments are only a part of the embodiments of the present invention, and not all of them. All other embodiments of the present invention can be obtained by those skilled in the art without creative efforts based on the embodiments of the present invention.
Referring to fig. 1-2, in an embodiment of the present invention, an apparatus for indoor bioassay of liriomyza trifoliate includes a water culture cup 1, a water culture cup cover 2, a cup cover lid 3, and a preservative film 4. The water culture cup 1 is provided with a water culture cup cover 2, and the top end face of the water culture cup cover 2 is provided with a ventilation port 2-1, so that the constant humidity in the water culture cup 1 and the air circulation in a bottle are ensured, and the sufficient oxygen for the respiratory growth of the liriomyza trifoliata can be ensured; a cup cover cap 3 is bonded on the ventilation opening 2-1, the cup cover cap 3 is of a net structure and is made of a 200-mesh nylon gauze, the purpose is to prevent the clover leaf-spot liriomyza from flying out of the device bottle to influence the experiment, and the cup cover cap 3 of the 200-mesh nylon gauze can play a role in air circulation; the preservative film 4 covers the cup mouth of the water culture cup 1, and holes are formed in the preservative film according to needs during use, so that survival of blades is guaranteed, humidity in the device is kept, and meanwhile pupas of liriomyza trifoliata are collected.
The ventilation port 2-1 on the water culture cup cover 2 is provided, a certain area (the area can be treated according to the size of the water culture cup cover 2) can be removed from the center by adopting a wallpaper knife or other tools, so that the air circulation in the device is ensured, and the death of the liriomyza sativae due to oxygen deficiency or air non-circulation in the device is prevented;
the water culture cup 1 and the water culture cup cover 2 are made of transparent plastic materials, so that observation and recording are facilitated; when the specification is selected, the caliber of the water culture cup cover 2 is larger than that of the water culture cup 1, and the water culture cup cover and the water culture cup are tightly combined as much as possible under the condition of ensuring the treatment requirement of air circulation; due to the matched consumables, the matched consumables can be freely selected according to the requirements and are suitable for specification matching.
Method of operation of the utility model
In the indoor biological measurement process, firstly, a water culture cup 1 is filled with clear water, which is about 2/3 parts of a cup body, and is sealed by a preservative film 4; the aperture of the water culture cup cover 2 is selected to be larger than that of the water culture cup 1, the combination of the two is proper, the treated water culture cup cover 2 is sealed by a cup cover 3 of a 200-mesh nylon gauze, and the water culture cup 1 and the water culture cup cover 2 are tightly combined to complete the installation and the use of the whole device; when indoor bioassay is carried out, after the cowpea seedlings with the wormholes are soaked in the medicine and dried, the redundant rootstocks are obliquely cut off below the stem ends and are moved into a device; a hole is poked in the center of the preservative film 4 in advance (the number and the size of the holes are non-limiting and are determined according to the experimental requirements and the growth vigor of plants), stems of cowpea seedlings can penetrate through the preservative film and be inserted into water conveniently, and finally, the treated water culture cup cover 2 is covered; in the experimental process, the state of the liriomyza trifoliate is observed and data are recorded after different times.
Example (b):
1. the length of the cowpea seedlings is about 10cm, 160ml of water culture cups 1 can be selected, 300ml of water culture cup covers 2 can be selected, and one cowpea seedling is placed in the device;
2. cowpea seedling length 15cm is left and right, and 200ml can be selected to water planting cup 1, and the specification of 2 water planting cups of water planting cup cover is: the caliber is 90mm, the height is 125mm, and three cowpea seedlings can be placed in the device.
Moreover, it is to be fully understood that although the description is provided in terms of specific embodiments, it is not intended that each embodiment be construed as an independent claim, and that each embodiment is provided solely for the purpose of illustration, which is for the purpose of clarity only, and that all embodiments shown and described herein are to be considered as examples and for all purposes only and are to be construed as limitations on the scope of the claims.
Claims (3)
1. The utility model provides an indoor bioassay device of cowpea pest clover spot latent fly, includes water planting cup (1), water planting cup cover (2), cup cover lid (3) and plastic wrap (4), its characterized in that: the water culture cup is characterized in that a water culture cup cover (2) is arranged on the water culture cup (1), a ventilation opening (2-1) is formed in the top end face of the water culture cup cover (2), a cup cover cap (3) is bonded to the ventilation opening (2-1), and the cup cover cap (3) is of a net structure; the preservative film (4) covers the cup mouth of the water culture cup (1).
2. The indoor bioassay device for the cowpea pest liriomyza sativae indoor as claimed in claim 1, characterized in that: the water culture cup (1) and the water culture cup cover (2) are made of transparent plastic materials, and the caliber of the water culture cup cover (2) is larger than that of the water culture cup (1).
3. The indoor bioassay device for the cowpea pest liriomyza sativae indoor as claimed in claim 1, characterized in that: the cup cover cap (3) is made of a 200-mesh nylon gauze.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202123330542.XU CN216601153U (en) | 2021-12-28 | 2021-12-28 | Indoor bioassay device for cowpea pest clover leaf liriomyza sativae |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202123330542.XU CN216601153U (en) | 2021-12-28 | 2021-12-28 | Indoor bioassay device for cowpea pest clover leaf liriomyza sativae |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN216601153U true CN216601153U (en) | 2022-05-27 |
Family
ID=81683620
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202123330542.XU Active CN216601153U (en) | 2021-12-28 | 2021-12-28 | Indoor bioassay device for cowpea pest clover leaf liriomyza sativae |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN216601153U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116076443A (en) * | 2022-07-29 | 2023-05-09 | 扬州大学 | Biological assay device, method and application of rice leaf roller larvae |
-
2021
- 2021-12-28 CN CN202123330542.XU patent/CN216601153U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116076443A (en) * | 2022-07-29 | 2023-05-09 | 扬州大学 | Biological assay device, method and application of rice leaf roller larvae |
| CN116076443B (en) * | 2022-07-29 | 2024-04-16 | 扬州大学 | Biological assay device, method and application of rice leaf roller larvae |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Shelton et al. | Comparative analysis of two rearing procedures for diamondback moth (Lepidoptera: Plutellidae) | |
| Subandi et al. | Suitability of Corcyra cephalonica eggs parasitized with Trichogramma japonicum as intermediate host against sugarcane borer Chilo auricilius. | |
| Barker et al. | Growing M. truncatula: choice of substrates and growth conditions | |
| CN106954592B (en) | Large-scale breeding method for Alliid lice spikemoss | |
| US7985412B2 (en) | Method of monitoring/controlling thysanoptera | |
| CN109315356B (en) | Hatching and feeding device suitable for whitefly or parasitic wasp insects and application method thereof | |
| CN216601153U (en) | Indoor bioassay device for cowpea pest clover leaf liriomyza sativae | |
| CN113519468B (en) | Method for feeding phyllotreta striolata in indoor generation | |
| CN108918785B (en) | Device and method for measuring toxicity of whitefly pests by using living plants | |
| CN107389870A (en) | It is a kind of that stomach toxicity and toxicity test device and method of tagging are carried out to Frankliniella occidentalis | |
| CN107810926A (en) | The artificial large-scale breeding method of Wan Shi Eimeria aythyaes | |
| Kasuga et al. | Seasonal prevalence and susceptibility to agrochemicals of Tyrophagus similis (Acari: Acaridae) in spinach buds and agricultural soil under greenhouse conditions | |
| CN208402950U (en) | A kind of device for preventing feed mouldy in Frankliniella occidentalis breeding process | |
| CN103125451A (en) | Method for propagating Exorista civilis Rondani | |
| Hussey et al. | The introduction of natural enemies for pest control in glasshouses: ecological considerations | |
| CN108684614A (en) | The device and application method for preventing feed mouldy in a kind of Frankliniella occidentalis feeding process | |
| Waite | New strategies to improve the efficiency of the biological control agent, Orius insidiosus (Say), in greenhouse ornamental crops | |
| CN210222030U (en) | Toxicity measuring device for fruit fly adult stomach toxicity | |
| CN104322454B (en) | Smaller green leaf hopper live body store method | |
| CN111183986A (en) | Pesticide containing diatomite and spinetoram and preparation method thereof | |
| CN109197786A (en) | A kind of method of interior mass rearing semi-closure initial bow | |
| CN110547303B (en) | Application of bacillus thuringiensis G033A in prevention and treatment of southern American tomato leaf miner | |
| Allsopp | Developing an integrated management system for western flower thrips, Frankliniella occidentalis (Pergande), on deciduous fruit, using semiochemicals in a push-pull strategy | |
| CN109845705B (en) | Small parasitic wasp adult stomach toxicity and contact toxicity measuring device and using method | |
| Freeman | Minnesota plant diseases |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant |