CN107389870A - It is a kind of that stomach toxicity and toxicity test device and method of tagging are carried out to Frankliniella occidentalis - Google Patents
It is a kind of that stomach toxicity and toxicity test device and method of tagging are carried out to Frankliniella occidentalis Download PDFInfo
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- CN107389870A CN107389870A CN201710681306.1A CN201710681306A CN107389870A CN 107389870 A CN107389870 A CN 107389870A CN 201710681306 A CN201710681306 A CN 201710681306A CN 107389870 A CN107389870 A CN 107389870A
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Abstract
The present invention relates to a kind of Frankliniella occidentalis stomach toxicity and tag toxicity test device and assay method, and device includes lid (1), base (2), and is fixedly installed on the lid gauze (3) in the middle part of lid (1)；Described lid (1) side wall inner edge or base (2) side wall outer rim is provided with flexible material；The middle part of base (2) is also provided with base gauze (8), and filter paper (5) is equipped with base gauze (8).Structure of the invention is simple, convenient to use, cost is cheap, flexibility is high, is remarkably improved efficiency reduces labor intensity, improves the accuracy of toxicity test.The present invention method have stomach toxicity and tag independent role or combination after while act on, and it is easy to operate, processing easily, the cycle it is relatively short, accuracy is accurate and detection efficiency is high.
Description
Technical field
The invention belongs to insect virulence assay device structures and toxicity test method and technology field, refer specifically to be exclusively used in western flower
The stomach toxicity of this insect of thrips and tag toxicity test device and toxicity test method and technology field.
Background technology
Frankliniella occidentalis [Frankliniellaoccidentalis (Pergande)] belongs to one kind of Thysanoptera flower thrips category
The gardening plant insects such as important global vegetables, flowers, it can cause blade, fruit rust brown spot occur, make fruit
Commodity is lost, in addition to directly endangering host plant, it is also possible to tomato spotted wilf virus disease and garden balsam necrotic spot virus are propagated,
Chemical prevention is mainly taken in the preventing and treating for Frankliniella occidentalis at present.
The toxicity test of insecticide is that a kind of quantity of toxicity represents, i.e. toxic degree.Purpose is intended to know a certain
Kind of insecticide to the toxicity of a certain insect how, or compare difference of the several pesticides to the virulence degree of a certain insect
Not, it is as weighing a kind of virulence size of insecticide to certain insect.To the west of insecticide is to the toxicity test of Frankliniella occidentalis
Flower thrips is test object, evaluates virulence of the various insecticides to Frankliniella occidentalis, toxicity pair of the measure insecticide to westernization thrips
It is of great significance in field control and physiological effect experiment.Therefore, it is raw in behavior of the research insecticide to Frankliniella occidentalis
During reason change and influence etc., it is often necessary to first carry out insecticide and the toxicity test of Frankliniella occidentalis is worked.But west flower Ji
Horse individual is quite small (a length of 1~2mm of body), and toxicity test process easily causes artificial experimental error.
Do not tagged in the prior art using the method do not sprayed directly because Frankliniella occidentalis individual 1~2mm it
Between, westernization thrips is easily dead in excessively moist environment, it is impossible to illustrates that insecticide acts on；Mainly use two kinds of toxicity tests
Method：Impregnate feeding method and gradient medicine embrane method.It is mainly stomach poison function to impregnate feeding method, and gradient medicine embrane method is action of contace poison, but
It is that both assay methods have larger error with field practical function, indoor virulence immediate can not reflect field reality
Result is tested, always stomach toxicity and tags while exists in field rotating medicine；Furthermore two methods are cumbersome, feed is not easy to locate
Reason, measure cycle length, typically at 48 hours or so, and contact efficacy is unsatisfactory in continuous mode, and experimental data has relative
Big error, operating process need larger labor intensity and time loss.Therefore designing one kind has stomach toxicity and tags individually
Effect or act on simultaneously, and it is easy to operate, processing easily, the Frankliniella occidentalis that the cycle is relatively short, accuracy is accurate, detection efficiency is high
The assay method of toxicity test corollary apparatus, it is the striving direction for solving existing situation, for this problem, we are to west flower Ji
The effective assay method of horse toxicity test corollary apparatus has carried out invention research.
The content of the invention
It is an object of the invention to provide a kind of Frankliniella occidentalis stomach toxicity and tag toxicity test corollary apparatus and virulence survey
Determine method (medicine pad feeding embrane method).
The present invention adopts the following technical scheme that realization.
A kind of that stomach toxicity and the device for toxicity test of tagging are carried out to Frankliniella occidentalis, the present invention includes lid, base, Yi Jigu
Surely the lid gauze being arranged in the middle part of lid；Described cap side-wall inner edge or base side wall outer rim is provided with flexible material；Bottom
The middle part of seat is also provided with base gauze, and filter paper is equipped with base gauze.Described flexible material can by lid with
Base covers so that the side wall of lid and base can tight closure, can so prevent this small elder brother of Frankliniella occidentalis
Worm thereby may be ensured that the reliability of experiment by being escaped in gap.The use of gauze can be beneficial to spraying insecticide to filter paper
On, and ventilated effect can be played.The use of filter paper has following benefit：1st, moisturizing, a secret are provided for Frankliniella occidentalis
Environment；2nd, enterprising accommodating existing agricultural chemicals or the new research and development agricultural chemicals of carrying out of filter paper is to Frankliniella occidentalis or the progress toxicity survey of other insects
Fixed experiment.
It is further that flexible material of the present invention is rubber strip or adhesive tape.Rubber strip or adhesive tape are ten
Divide reliable flexible material, above-mentioned requirement of experiment can be met completely, and rubber strip or adhesive tape are usually to live
Conventional article, it is cheap, it is easy to oneself processing manually.
It is further that the mesh number of lid gauze and base gauze is the mesh of 120 mesh~160.Due to the individual ten of Frankliniella occidentalis
It is point small, so can cause Frankliniella occidentalis that escape incident occurs if the gauze less than 120 mesh is selected, if easy more than 160 mesh
Cause airtight generation thrips death incident in device.
It is further that circular or rectangle is shaped as after described lid and base engagement.Common laboratory often uses
To the culture dish of circle, then culture dish can manually improve so as to fit Frankliniella occidentalis when using circular culture dish
Toxicity test requirement of experiment；And when being used as production, for convenience of packing, it is not easy to rock, then can uses the design of rectangle,
Such design more can conveniently transport for long-distance.
It is further that described lid lateral wall is provided with projection.Can due to being brought into close contact for flexible material, during opening
Can be a little difficult, and the increase of projection solves this trouble, can cause very convenient, laborsaving when opening lid.
It is further that described cap top is provided with rubber band, described rubber band both ends are fixedly installed on cap top two
End.Often adhere to some Frankliniella occidentalis during raising, on gauze, it is necessary to open lid to Frankliniella occidentalis carry out investigation statisticses when,
Slight springing can be carried out to gauze with the rubber band of cap top, the Frankliniella occidentalis bullet on gauze is fallen to filter paper.
It is further that the device specification is circular design：The size of described lid：Lid diameter 10cm, thick 0.1cm are high
1.2cm, gauze diameter 5cm；The size of base：Overall diameter 9.9cm, interior diameter 9.6cm, high 1.5cm, rubber strip or adhesive tape
Wide 1.5cm, thick 0.2cm；Bottom gauze diameter 9cm；The a diameter of 9.5cm of filter paper.
It is further that the device specification is squared design：The size of described lid：Long 10cm × wide 10cm is thick
0.1cm, high 1.2cm, gauze diameter 5cm；The size of base：The long 9.9cm of outer rim × wide 9.9cm, the long 9.6cm in inner edge × wide
9.6cm, high 1.5cm, rubber strip or transparent adhesive tape bandwidth 1.5cm, thick 0.2cm；Directly long 9cm × wide 9cm, filter paper are length to bottom gauze
9.5cm × wide 9.5cm.
The height of lid is slightly less than the height of base, such to can be designed so that when opening lid, and convenience is held
Firmly base is with more firm.The inside dimension for being dimensioned slightly smaller than base of filter paper, if filter paper is slightly larger than the inside dimension of base, need
Some folds are carried out, and this fold is the place that Frankliniella occidentalis is often concealed, and is unfavorable for the later stage system of toxicity test experiment
Meter.
A kind of method of the toxicity test for the device that toxicity test is carried out to Frankliniella occidentalis, the present invention comprise the following steps：
Step 1, insecticide is configured to various concentrations gradient decoction, and by the bottom gauze of filter paper pads to corollary apparatus
On；
Step 2, Kidney bean is soaked into 5min in decoction, take out after room temperature is dried and be placed on the filter paper of device, west flower Ji
Horse is linked into corollary apparatus by each 50 thrips of corollary apparatus and covers lid；
Step 3, insecticide is sprayed onto on filter paper from the gauze of base with small watering can, insecticide is uniformly bedewed filter paper
It is but non-condensing into hydrops；
Step 4, it is placed on after being raised 24 hours in growth cabinet and observes survival condition, data is carried out using SPSS22.0
Treatment Analysis, obtain virulence regression equation and lethal concentration of 50 LC50；
Described insecticide is configured to：
First insecticide is measured the decoction that larger concentration is configured in clear water is dissolved in after conversion weighs, then diluted
Decoction to 6~8 constant gradient concentration is contained in culture dish；
Described Kidney bean is：
Kidney bean (Phaseolus vulgaris L.) for examination phaseolus vulgaris seeds from the stronger kind of resistance, at insecticide
It is not mouldy after reason, such as U.S.'s provider's kind, the supply of Nanjing ideal seedling Co., Ltd, by after presprouting of seeds in greenhouse, plant
Plant in nylon gauze in greenhouse, prevent insect infestations；During experiment, a water is poured within every 2~3 days, until being adopted during Kidney bean maturation
Pluck and tested；Any agricultural chemicals is not sprayed during experiment, Kidney bean grows fine；
The processing of the insecticide immersion Kidney bean：
The section that Kidney bean is cut into equal length is put into the culture dish of various concentrations decoction, is taken out, made with tweezers after soaking 5min
Kidney bean surface forms one layer of medicine film, and Kidney bean is moved into drying at room temperature for lucifuge with tweezers, is put on the filter paper in corollary apparatus；
Described insecticide is to the processing that filter paper squirts：
It is 24mm with bore, capacity is 200ml 6, small watering can, is respectively sprayed onto concentration liquid from the gauze of bottom
On the filter paper of corollary apparatus, distance controlling makes filter paper monoblock soak but non-condensing into hydrops in 10~20cm during sprinkling；Further
For Frankliniella occidentalis of the present invention rearing conditions in growth cabinet are that temperature is 26~28 DEG C, periodicity of illumination L:D=
16:8, raising humidity is 60%~70%.
Beneficial effects of the present invention are：The said structure that the device of Frankliniella occidentalis toxicity test of the present invention uses, for each
Toxicity test of the kind insecticide to Frankliniella occidentalis.Circular gauze on lid is fixed on lid, solves in experimentation and has
The loss and operating personnel that the thrips for having brill moth can escape need to remove the operation inconvenience brought every time；Circular gauze can meet
Air circulation is smooth, solves existing apparatus due to closed so as to cause the experimental error that Frankliniella occidentalis airless zone comes；Device bottom
Rubber strip or adhesive tape in seat side wall perfectly combine with lid makes it have certain elasticity, and solving base can not be fine
Be close to lid and make to have the Frankliniella occidentalis of brill moth escape caused by experimental error.The filter paper that bottom is lined with can solve
Certainly human error caused by the cleaning difficulty and residue problem of the different evaluations of pesticides every time, and it is good to play action of contace poison
Solves the non-type problem of the insecticide assay method of stage action of contace poison now.The present invention can meet the different mechanisms of action
Insecticide can be used for the toxicity test for determining other mini pests to Frankliniella occidentalis toxicity test；Hollow of device can be with
Put relatively large toxicity test feed.The present invention is simple in construction, convenient to use, cost is cheap, flexibility is high, can be notable
Raising labour efficiency reduces labor intensity, improves the accuracy of toxicity test.
The Frankliniella occidentalis stomach toxicity of the present invention and tag toxicity test method, toxicity test is relative with lid and base
Determined in the device of closing, solve error caused by Frankliniella occidentalis escape；Insecticide is sprayed onto on filter paper from bottom gauze and solved
Determine and be first put into caused by filter paper places into westernization thrips insecticide and evaporate, touched using be first put into that Frankliniella occidentalis sprayed again
The effect of killing, it is possible to reduce thrips escapes and artificially shakes the error having on insecticide filter paper, it is possible to achieve some insecticide poison
Property measure when repeatedly spray and change filter paper and device for opening repeatedly without period；Kidney bean is immersed in insecticide and forms medicine film
After take out, error of the thrips caused by westernization thrips food not in contact with insecticide can not be attached to by solving decoction, improve essence
Exactness.The method of the present invention acts on while having stomach toxicity with after tag independent role or combination, and easy to operate, processing is held
Easily, the cycle is relatively short, and accuracy is accurate and detection efficiency is high.Operating personnel easily grasp, and are especially easy for a variety of insecticides pair
The toxicity test of thrips, even if operating personnel do not have relevant professional knowledge, it can also learn in a short time.The assay method of the present invention
It can be adapted to be widely popularized in major plant protection unit, can be the development and the quantitative effective control of agricultural chemicals of China's agricultural chemicals cause
System brings significant directiveness effect, and solid theoretical foundation is laid for quickly measuring accurately experimental result, it is final for
Minimum applications of pesticide amount reaches maximum insecticidal effect, so maximum magnitude reduce to the harm of human body and be provided with and try hard to keep
Barrier.
The present invention is further explained with reference to the accompanying drawings and detailed description.
Brief description of the drawings
Fig. 1 is the structural representation of the present invention.
In figure marked as：1. lid, 2. bases, 3. lid gauzes, 4. rubber strips or adhesive tape, 5. filter paper, 6. projections,
7. rubber band, 8, base gauze.
Embodiment
In order that methods of this invention will be better understood by those skilled in the art, with reference to specific embodiment to this hair
It is bright to be described in further detail.
A kind of that stomach toxicity and the device for toxicity test of tagging are carried out to Frankliniella occidentalis, the present invention includes lid 1, base 2, and
It is fixedly installed on the lid gauze 3 at the middle part of lid 1；The described side wall inner edge of lid 1 or the side wall outer rim of base 2 set flexible material
Matter；The middle part of base 2 is also provided with base gauze 8, and filter paper 5 is equipped with base gauze 8.Described flexible material can lead to
Cross covering for lid 1 and base 2 so that the side wall of lid 1 and base 2 can tight closure, can so prevent Frankliniella occidentalis
This small insect thereby may be ensured that the reliability of experiment by being escaped in gap.The use of gauze can be beneficial to sprinkling
Insecticide can play ventilated effect on filter paper 5.The use of filter paper 5 has following benefit：1st, provided for Frankliniella occidentalis
One moisturizing, concealed environment；2nd, enterprising accommodating existing agricultural chemicals or the new research and development agricultural chemicals of carrying out of filter paper 5 is to Frankliniella occidentalis or other
Insect carries out toxicity test experiment.
It is further that flexible material of the present invention is rubber strip or adhesive tape 4.Rubber strip or adhesive tape 4 are
Very reliable flexible material, above-mentioned requirement of experiment can be met completely, and rubber strip or adhesive tape 4 are usually raw
Conventional article living, it is cheap, it is easy to oneself processing manually.
It is further that the mesh number of lid gauze 3 and base gauze 8 is the mesh of 120 mesh~160.Due to the individual of Frankliniella occidentalis
It is very small, so can cause Frankliniella occidentalis that escape incident occurs if the gauze less than 120 mesh is selected, if holding more than 160 mesh
Easily cause airtight generation thrips death incident in device.
It is further to be shaped as circular or rectangle after described lid 1 and the cooperation of base 2.Common laboratory often makes
The culture dish of circle is used, can then carry out artificial improve so as to fit west flower Ji to culture dish when using circular culture dish
The toxicity test requirement of experiment of horse；And when being used as production, for convenience of packing, it is not easy to rock, then can uses setting for rectangle
Meter, such design more can conveniently transport for long-distance.
It is further that the described lateral wall of lid 1 is provided with projection 6.Due to being brought into close contact for flexible material, during opening
May be a little difficult, and the increase of projection 6 solves this trouble, can cause the very convenient, province when opening lid 1
Power.
It is further that the described top of lid 1 is provided with rubber band 7, and the described both ends of rubber band 7 are fixedly installed on lid 1 and pushed up
Portion both ends.Often adhere to some Frankliniella occidentalis during raising, on gauze, it is necessary to which open lid 1 carries out investigation system to Frankliniella occidentalis
Timing, slight springing can be carried out to gauze with the rubber band 7 at the top of lid 1, the Frankliniella occidentalis bullet on gauze is fallen to filter paper 5
On.
It is further that the device specification is circular design：The size of described lid 1：Lid diameter 10cm, thick 0.1cm,
High 1.2cm, gauze diameter 5cm；The size of base 2：Overall diameter 9.9cm, interior diameter 9.6cm, high 1.5cm, rubber strip or transparent
Adhesive tape 4 wide 1.5cm, thick 0.2cm；Bottom gauze diameter 9cm；5 a diameter of 9.5cm of filter paper.
It is further that the device specification is squared design：The size of described lid 1：Long 10cm × wide 10cm is thick
0.1cm, high 1.2cm, gauze diameter 5cm；The size of base 2：The long 9.9cm of outer rim × wide 9.9cm, the long 9.6cm in inner edge × wide
9.6cm, high 1.5cm, rubber strip or adhesive tape 4 wide 1.5cm, thick 0.2cm；Directly long 9cm × wide 9cm, filter paper 5 are bottom gauze
Long 9.5cm × wide 9.5cm.
The height of lid 1 is slightly less than the height of base 2, such to can be designed so that when opening lid 1, conveniently uses hand
Base 2 is held with more firm.The inside dimension for being dimensioned slightly smaller than base 2 of filter paper 5, if filter paper 5 is slightly larger than the inside chi of base 2
, it is necessary to carry out some folds when very little, and this fold place that to be Frankliniella occidentalis often conceal, it is unfavorable for toxicity test experiment
Later stage counts.
A kind of method for the toxicity test that stomach toxicity and the device for toxicity test of tagging are carried out to Frankliniella occidentalis, the present invention include
Following steps：
Step 1, insecticide is configured to various concentrations gradient decoction, and by the bottom gauze of filter paper pads to corollary apparatus
On；
Step 2, Kidney bean is soaked into 5min in decoction, take out after room temperature is dried and be placed on the filter paper of device, by west flower
Thrips is linked into corollary apparatus by each 50 thrips of corollary apparatus and covers lid；
Step 3, insecticide is sprayed onto on filter paper from the gauze of base with small watering can, insecticide is uniformly bedewed filter paper
It is but non-condensing into hydrops；
Step 4, it is placed on after being raised 24 hours in growth cabinet and observes survival condition, data is carried out using SPSS22.0
Treatment Analysis, obtain virulence regression equation and lethal concentration of 50 LC50；
Described insecticide is configured to：
First insecticide is measured the decoction that larger concentration is configured in clear water is dissolved in after conversion weighs, then diluted
Decoction to 6~8 constant gradient concentration is contained in culture dish；
Described Kidney bean is：
Kidney bean (Phaseolus vulgaris L.) for examination phaseolus vulgaris seeds from the stronger kind of resistance, at insecticide
It is not mouldy after reason, such as U.S.'s provider's kind, the supply of Nanjing ideal seedling Co., Ltd, by after presprouting of seeds in greenhouse, plant
Plant in nylon gauze in greenhouse, prevent insect infestations；During experiment, a water is poured within every 2~3 days, until being adopted during Kidney bean maturation
Pluck to thrips to raise in cylinder and tested；Any agricultural chemicals is not sprayed during experiment, Kidney bean grows fine；
The processing of the insecticide immersion Kidney bean：
Kidney bean is put into and is cut into the section of equal length and is put into the culture dish of various concentrations decoction, is taken after soaking 5min with tweezers
Go out, Kidney bean surface is formed one layer of medicine film, Kidney bean is moved into drying at room temperature for lucifuge, the filter being put into corollary apparatus with tweezers
On paper；
Described insecticide is to the processing that filter paper squirts：
It is 24mm with bore, capacity is 200ml 6, small watering can, is respectively sprayed onto concentration liquid from the gauze of bottom
On the filter paper of corollary apparatus, distance controlling makes filter paper monoblock soak but non-condensing into hydrops in 10~20cm during sprinkling；Further
For Frankliniella occidentalis of the present invention rearing conditions in growth cabinet are 26~28 DEG C of temperature, periodicity of illumination L:D=16:
8, raising humidity is 60%~70%.
Specific operation process
(1) corollary apparatus is：
Formed including the transparent plastic lid with gauze and bottom for the transparent plastic base with rubber strip of gauze, it is described
Transparent plastic lid the diameter 10cm, high 1.2cm, thick 0.1cm with gauze, described gauze it is fine and closely woven degree be 120 mesh~160
Mesh, lid gauze diameter 5m；Described base overall diameter 9.9cm, interior diameter 9.6cm, high 1.5cm, rubber in described side wall
Bandwidth 1.5cm, thick 0.2cm；Described bottom gauze diameter 9cm, fine and closely woven degree are the mesh of 120 mesh~160, gauze in described base
The a diameter of 9.5cm of filter paper of upper laying；
(2) experiment condition：
Experiment is carried out in growth cabinet, and growth cabinet (rising instrument, LTC-1000 in Shanghai three) condition setting is as follows：
Temperature is 26~28 DEG C, periodicity of illumination L:D=16:8, raising humidity is 60%~70%；
(3) for examination Kidney bean
Kidney bean (Phaseolus vulgaris L.) for examination phaseolus vulgaris seeds from the stronger kind of resistance, at insecticide
It is not mouldy after reason, by after presprouting of seeds in greenhouse, plant in nylon gauze in greenhouse, prevent insect infestations；During experiment,
Pour a water within every 2~3 days, tested until plucking to thrips to raise in cylinder during Kidney bean maturation；Do not sprayed during experiment any
Agricultural chemicals, Kidney bean grow fine；
(4) selected insect source
Population is established in laboratory progress Identification of Species for the Frankliniella occidentalis worm sources of examination, chooses the healthy adult of thrips.Will
Thrips carries out 5 with Kidney bean in growth cabinet and more than generation purifies raising.The pseudo- pupa of thrips is collected before experiment, treats that pseudo- pupa is sprouted wings
Afterwards, the Frankliniella occidentalis thrips adult to sprout wings on the same day is collected with pooter to be for experiment；
(5) insecticide is configured to：
First insecticide is measured the decoction that larger concentration is configured in clear water is dissolved in after conversion weighs, then diluted
Decoction to 6~8 constant gradient concentration is contained in culture dish；
(6) processing of insecticide immersion Kidney bean：
Kidney bean is put into and is cut into the section of equal length and is put into the culture dish of various concentrations decoction, is taken after soaking 5min with tweezers
Go out, Kidney bean surface is formed one layer of medicine film, Kidney bean is moved into drying at room temperature for lucifuge, the filter being put into corollary apparatus with tweezers
On paper；
(7) Frankliniella occidentalis is linked into corollary apparatus by each 50 Frankliniella occidentalis adults of corollary apparatus and covers lid；
(8) insecticide is to the processing that filter paper squirts：
It is 24mm with bore, capacity is 200ml 6, small watering can, is respectively sprayed onto concentration liquid from the gauze of bottom
On the filter paper of corollary apparatus, distance controlling makes filter paper monoblock soak but non-condensing into hydrops in 10~20cm during sprinkling；
(9) it is placed on after being raised 24 hours in growth cabinet and observes survival condition；
(10) morphological feature of Frankliniella occidentalis：
Female adult pest:Body grows 1.4~1.7mm, and body colour is faint yellow to brown, and variation is larger, and feeler 8 saves, and Section 1 is light yellow,
Section 2 brown, the 3rd~5 section yellow, but end is slightly brown, the 6th~8 section brown.There is forked sense cone on 3rd, 4 sections.The head breadth is bigger
In long, write before simple eye shorter, it is simple eye soft 1 pair, very long, fresh 6 pairs behind the inner side of front and rear simple eye outer line, compound eye,
It is 4th pair in riotous profusion most long from inside to outside, it is about isometric with simple eye hair on the neck.Pronotary has 4 pairs of long collection, respectively positioned at leading edge, left and right anterior angle
Each 1 pair, left and right relief angle 2 is right, trailing edge center have 5 pairs it is in riotous profusion, wherein from center it is outside 2nd pair in riotous profusion most long；Middle metanotum healing, after
There is irregular netted line in thoracic dorsal plate center, has a pair of mitriform perceptrons (being in bright spot shape) close to rear, in metanotum leading edge
There are 2 pairs of long hairs on the neck centre.Fore wing is faint yellow, upper arteries and veins ticket 18~21, lower 13~16 in riotous profusion of arteries and veins, and arrangement is uniformly complete.Belly the 5th~8
There is micro-bend comb section backboard both sides, and the 8th backboard trailing edge has 12 1 skilful root of pectination hair.Section 9 backboard has 2 pairs of mitriform perceptrons.3rd~
7 web trailing edges have 3 pairs in riotous profusion, without attached hair on the neck.
Male imago:Body grows 1~1.15mm, body yellow, and the apotome of belly the 3rd~7 has a small horizontal oval gland room, and the 8th
Backboard trailing edge is saved without pectination hair.
(10) the lower three kinds of insecticides of equal conditions raise embrane method to Frankliniella occidentalis toxicity test using medicine pad
1 three kinds of insecticides of table raise result of the embrane method to Frankliniella occidentalis adult toxicity test using medicine pad
Result of the test (table 1) shows, in the Frankliniella occidentalis adult toxicity test result that embrane method measure is raised using medicine pad, for examination
In medicament, to Frankliniella occidentalis adult virulence by up to bottom be AVM, decis, imidacloprid, the LC of its virulence50Value difference
For 3.724,9.606,171.274, coefficient correlation is respectively 0.991,0.988,0.983, and coefficient correlation is above 0.980.Table
Bright Frankliniella occidentalis theoretical death rate is significantly coincide with actual, and gained virulence regression equation preferably reflects corresponding medicine
Indoor virulence of the agent to Frankliniella occidentalis.
(11) three kinds of insecticides are using dipping feeding method to Frankliniella occidentalis toxicity test
Result of the 2 three kinds of insecticides of table using dipping feeding method to Frankliniella occidentalis adult toxicity test
Result of the test (table 2) shows, in the Frankliniella occidentalis adult toxicity test result using dipping feeding method measure, for examination
Medicament is AVM by up to bottom to Frankliniella occidentalis adult virulence, decis, imidacloprid, the LC of its virulence50Value is respectively
3.261st, 10.589,173.285, coefficient correlation is respectively 0.963,0.971,0.975, and coefficient correlation is above 0.960.With this
Invention medicine pad raises the coefficient correlation slightly difference in the Frankliniella occidentalis adult toxicity test result of embrane method measure, gained toxicity regression
A small amount of error be present in equation.
(12) three kinds of insecticides are using gradient medicine embrane method to Frankliniella occidentalis toxicity test
Result of the 3 three kinds of insecticides of table using gradient medicine embrane method to Frankliniella occidentalis adult toxicity test
Result of the test (table 3) shows, in the Frankliniella occidentalis adult toxicity test result using gradient medicine embrane method measure, for examination
Medicament is AVM by up to bottom to Frankliniella occidentalis adult virulence, decis, imidacloprid, the LC of its virulence50Value is respectively
3.724th, 10.369,177.183, coefficient correlation is respectively 0.915,0.925,0.928, and coefficient correlation is above 0.910.With this
Difference, gained toxicity regression be present in the coefficient correlation that invention medicine pad is raised in the Frankliniella occidentalis adult toxicity test result of embrane method measure
Error be present in equation.
The comparison of (13) three kinds of pesticide toxicity evaluation method acquired results
Results contrast of the 4 three kinds of insecticides of table using three kinds of assay methods to Frankliniella occidentalis adult toxicity test
Three kinds of insecticides are shown the comparative result (table 4) of Frankliniella occidentalis adult toxicity test using three kinds of assay methods,
Frankliniella occidentalis toxicity test method is used in the measure of three kinds of insecticides, showing the most strong insecticide of virulence using three kinds
For AVM, the most weak insecticide of toxicity is imidacloprid；Use what three kinds of assay methods obtained during using identical insecticide
In virulence regression equation, embrane method is raised using medicine pad of the present invention and determines coefficient correlation highest in obtained virulence regression equation, is used
It is minimum that gradient medicine embrane method determines coefficient correlation in obtained virulence regression equation.Virulence regression equation obtained by assay method of the present invention
Preferably reflect indoor virulence of the corresponding medicament to Frankliniella occidentalis.
The adult toxicity test process and result of Frankliniella occidentalis are described in detail above, but utilize the present invention's
Method can not only carry out toxicity test to the adult of Frankliniella occidentalis, also equally can carry out toxicity test to the nymph of Frankliniella occidentalis,
Most efficiently in the case of obtain the toxicity test results of different worm states；And to shaped like Frankliniella occidentalis it is such have brill moth,
Small, the movable rapidly insect of individual can also carry out the toxicity test of adult or nymph using this inventive method.
Although above the present invention is described in detail with a general description of the specific embodiments,
On the basis of the present invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Cause
This, in small these modifications or improvements on the basis of deviateing present invention spirit, unless playing significant innovation, all should belong to
The scope of protection of present invention.
Claims (10)
1. a kind of carry out stomach toxicity and the device for toxicity test of tagging to Frankliniella occidentalis, it is characterised in that including lid (1), base
, and the lid gauze (3) that is fixedly installed in the middle part of lid (1) (2)；Described lid (1) side wall inner edge or base (2) side wall
Outer rim is provided with flexible material；The middle part of base (2) is also provided with base gauze (8), and filter paper is equipped with base gauze (8)
(5)。
A kind of stomach toxicity is carried out to Frankliniella occidentalis and the device of toxicity test of tagging, its feature exist 2. according to claim 1
In described flexible material is rubber strip or adhesive tape (4).
A kind of stomach toxicity is carried out to Frankliniella occidentalis and the device of toxicity test of tagging, its feature exist 3. according to claim 1
In, the mesh number of lid gauze (3) and base gauze (8) be the mesh of 120 mesh~160.
A kind of stomach toxicity is carried out to Frankliniella occidentalis and the device of toxicity test of tagging, its feature exist 4. according to claim 1
Circular or rectangle is shaped as after, described lid (1) and base (2) coordinate.
A kind of stomach toxicity is carried out to Frankliniella occidentalis and the device of toxicity test of tagging, its feature exist 5. according to claim 1
In described lid (1) lateral wall is provided with projection (6).
A kind of stomach toxicity is carried out to Frankliniella occidentalis and the device of toxicity test of tagging, its feature exist 6. according to claim 1
In described lid (1) top is provided with rubber band (7), and described rubber band (7) both ends are fixedly installed on cap top both ends.
A kind of stomach toxicity is carried out to Frankliniella occidentalis and the device of toxicity test of tagging, its feature exist 7. according to claim 1
In the device specification is the size of described lid (1)：Lid diameter 10cm, high 0.5cm, thick 0.1cm, high 1.2cm, lid yarn
Net (3) diameter 5cm；The size of base (2)：Overall diameter 9.9cm, interior diameter 9.6cm, high 1.5cm, rubber strip or adhesive tape
(4) wide 1.5cm, thick 0.2cm；Filter paper (5) diameter 9.5cm；Filter paper (5) a diameter of 9.5cm；Base gauze (8) diameter 9cm.
A kind of stomach toxicity is carried out to Frankliniella occidentalis and the device of toxicity test of tagging, its feature exist 8. according to claim 1
In the device specification is the size of described lid (1)：Long 10cm × wide 10cm, thick 0.1cm, high 1.2cm, lid gauze
(3) diameter 5cm；The size of base (2)：The long 9.9cm of outer rim × wide 9.9cm, the long 9.6cm in inner edge × wide 9.6cm, high 1.5cm, rubber
Adhesive tape or adhesive tape (4) wide 1.5cm, thick 0.2cm；Filter paper (5) is long 9.5cm × wide 9.5cm；Base gauze (8) diameter
9cm。
A kind of 9. toxicity test that stomach toxicity and the device for toxicity test of tagging are carried out to Frankliniella occidentalis described in usage right requirement 1
Method, it is characterized in that：Comprise the following steps：
Step 1, insecticide is configured to various concentrations gradient decoction, and by the bottom gauze of filter paper pads to corollary apparatus；
Step 2, Kidney bean is soaked into 5min in decoction, take out after room temperature is dried and be placed on the filter paper of device, Frankliniella occidentalis is pressed
Each 50 thrips of corollary apparatus, which are linked into corollary apparatus, covers lid；
Step 3, insecticide is sprayed onto on filter paper from the gauze of base with small watering can, insecticide is uniformly bedewed filter paper but not
Condense into hydrops；
Step 4, it is placed on after being raised 24 hours in growth cabinet and observes survival condition, data processing is carried out using SPSS22.0
Analysis, obtain virulence regression equation and lethal concentration of 50 LC50；
Described insecticide is configured to：
First insecticide is measured the decoction that larger concentration is configured in clear water is dissolved in after conversion weighs, be then diluted to 6
The decoction of~8 constant gradient concentration is contained in culture dish；
Described insecticide is to the processing that filter paper squirts：
It is 24mm with bore, capacity is 200ml 6, small watering can, is respectively sprayed onto concentration liquid from the gauze of bottom supporting
On the filter paper of device, distance controlling makes filter paper monoblock soak but non-condensing into hydrops in 10~20cm during sprinkling；
Described Kidney bean is：
It is not mouldy after pesticide treatments for examination phaseolus vulgaris seeds from the stronger kind of resistance, by after presprouting of seeds in greenhouse,
Plant in nylon gauze in greenhouse, prevent insect infestations；During experiment, a water is poured within every 2~3 days, until during Kidney bean maturation
Harvesting is raised in cylinder to thrips and tested；Any agricultural chemicals is not sprayed during experiment, Kidney bean grows fine；
The processing of the insecticide immersion Kidney bean：
Kidney bean is put into and is cut into the section of equal length and is put into the culture dish of various concentrations decoction, is taken out, made with tweezers after soaking 5min
Kidney bean surface forms one layer of medicine film, and Kidney bean is moved into drying at room temperature for lucifuge with tweezers, is put on the filter paper in corollary apparatus.
10. a kind of Frankliniella occidentalis stomach toxicity and toxicity test method of tagging as described in claim 9, it is characterised in that described
Frankliniella occidentalis rearing conditions in growth cabinet are that temperature is 26~28 DEG C, periodicity of illumination L:D=16:8, raising humidity is
60%~70%.
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