CN102511409A - Device for breeding bees and application - Google Patents
Device for breeding bees and application Download PDFInfo
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- CN102511409A CN102511409A CN2011103954446A CN201110395444A CN102511409A CN 102511409 A CN102511409 A CN 102511409A CN 2011103954446 A CN2011103954446 A CN 2011103954446A CN 201110395444 A CN201110395444 A CN 201110395444A CN 102511409 A CN102511409 A CN 102511409A
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- essential oil
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Abstract
The invention provides a device for breeding bees, which is composed of a bee movement area and an essential oil placing area, wherein the bee movement area and the essential oil placing area are separated through a mesh. The mesh is fixed at the bottom of the bee movement area, a centrifugal tube with a cover is installed at the top of the bee movement area and used for accommodating feeding food, the bottom of the centrifugal tube is provided with an opening, and the diameter of the opening is 2-3mm. The top of the bee movement area is provided with an air vent sealed with a net, filter paper is horizontally paved at the bottom of the essential oil placing area and used for loading rosewood heart wood essential oil. The device enables the bees to freely feed, enables feeding to be convenient, bee mites can freely parasitize the bee bodies, the device is divided into an upper layer and a lower layer, the plant essential oil is separated from the bees and can act on the bees and the bee mites in a fuming mode, the mite killing effect is good, the bees are not hurt, toxicity to the bees is small, and toxicity of the plant essential oil on the bees and the bee mites can be measured simultaneously. The device for breeding the bees can be applied to experiments for screening anti-mite essential oil.
Description
Technical field
The invention belongs to the device of raising honeybee, relate to a kind of device of testing raising honeybee, and the application of this device in the anti-mite Obtaining Perfume Essential Oil of screening.
Background technology
Honeybee be under the jurisdiction of Arthropoda (Arthropoda), Insecta (Insecta), Hymenoptera (Hymenoptera), Apoidea (Apoidea), Apidae (Apidae), Apis (
Apis), be the highly developed economic type insect of a kind of social behavior.When the bee product with high nutritive value was provided very much, the economy that its pollination brings and the ecological value were inestimable especially for human for honeybee.
The Di Siwa mite (
Varroa destructorAnderson & Trueman 2000, common name " varoa mite ") be a kind of ectoparasite mite of honeybee, be world's apiculture is threatened maximum honeybee damage by disease and insect.The honeybee body weight that infects the Di Siwa mite obviously alleviates, even developmental deformity, and a series of symptoms can appear in bee colony, incomplete like young honeybee wing foot, disorderly climb everywhere, the dead dead pupa of honeybee everywhere, the decline etc. rapidly of crowd's gesture.In addition, it still is the carrier and the disseminator of a lot of cause of diseases.The co-infected of watt mite and multiple cause of disease has more harmfulness than any single virus infections, can cause the destructive injury of bee colony.If bee colony is not periodically controlled the mite scheme, most of bee colony in area, temperate zone will stride the crowd in 2～3 years.Therefore, annual control watt mite remains under the bee colony economic threshold a watt mite number, does not influence bee-keeping and produces, and has become important measures of bee-keeping management.In addition, the chalcid fly mite (
Tropilaelaps clareae) also be a kind of important parasite of honeybee.
Chemical miticides such as taufluvalinate, fluorochlorobenzene Cyano chrysanthemate, Amitraz are that the first-selection that goes up control honeybee mite is produced in bee-keeping always.But the phenomenon that medicament residue problem in the bee product that these chemical acaricidal application cause and the resistance of honeybee mite constantly strengthen makes the medicine of control honeybee mite of development of new become apiculture and studies instant task.
Essence oil from plant has wide material sources, low-residual, be difficult for causing advantage such as drug resistance, is the important directions of miticide development.But, in numerous essence oil from plant, have only few species that the honeybee mite is had toxic action, and wherein most of also toxic to honeybee.Therefore, from numerous essence oil from plant, filtering out has toxic action but the kind of or low toxicity nontoxic to honeybee to the honeybee mite, is the acaricidal essential step of exploitation plant resource.The appropriate device that raising honeybee under a kind of laboratory condition is provided is the key in this step.This device need meet following requirement: (1) honeybee ability free choice feeding; (2) the honeybee mite can be free on one's body parasitic honeybee; (3) essence oil from plant can act on honeybee and honeybee mite through stifling mode.
Summary of the invention
The device that the purpose of this invention is to provide a kind of raising honeybee, this device is made up of honeybee behaviour area and essential oil rest area, and honeybee behaviour area and essential oil rest area are separated through gauze; Gauze is fixed on bottom, honeybee behaviour area, and top, honeybee behaviour area one side is equipped with a centrifuge tube with cover, is used to adorn the liquid or the semifluid food (syrup or honey) of feeding honeybee; This centrifuge tube bottom is an opening, and opening diameter is 2-3mm, and top, honeybee behaviour area opposite side is provided with the blow vent that a usefulness net is sealed; Both reached circulation of air; Prevent the honeybee escape again, tiling filter paper in essential oil rest area bottom is used to load dalbergia wood essential oil.Honeybee behaviour area and essential oil rest area agree with, and can freely dismantle.
Another object of the present invention provides the application of said device in the anti-mite Obtaining Perfume Essential Oil of screening.Concrete grammar is: put into 20 honeybees and 10 honeybee mites in the honeybee behaviour area, the food of in centrifuge tube, packing into; A certain amount of essence oil droplet after on the filter paper of essential oil rest area, is connected honeybee behaviour area and essential oil rest area immediately, the poisoning symptom of honeybee and honeybee mite in the observed and recorded 1 hour, the lethality of per 12 hour record honeybees and honeybee mite was until 48 hours.Selection causes the exploitation that honeybee mite lethality is high, the bee death rate is low essential oil is used for miticide.
The present invention is directed to the extremely requirement of experiment of mite, design comprises honeybee behaviour area and the device that the two-layer container of essential oil rest area agrees with, and has following characteristics: (1) honeybee can free choice feeding, and the honeybee mite can be at the free on one's body parasitism of honeybee; (2) can add food through the tubule at top, honeybee behaviour area, feed conveniently; (3) device is two-layer about dividing, and essence oil from plant and honeybee isolate, and can act on honeybee and honeybee mite through stifling mode; (4) can measure the toxicity of essence oil from plant simultaneously to honeybee and honeybee mite.
Description of drawings
Fig. 1 is apparatus of the present invention structural representation.
Embodiment
The present invention combines accompanying drawing and embodiment to be further described.
Embodiment one
Referring to Fig. 1, apparatus of the present invention by honeybee behaviour area 1 and essential oil rest area 2 about two containers forms, gauze 6 separations are passed through with essential oil rest area 2 in honeybee behaviour area 1; Gauze 6 is fixed on 1 bottom, honeybee behaviour area, and 1 top, one side is equipped with a centrifuge tube with cover 3 in the honeybee behaviour area, is used to adorn the liquid or the semifluid food (syrup or honey) of feeding honeybee; These centrifuge tube 3 bottoms are tiny opening, and diameter can not be excessive between 2-3mm; In order to avoid the food in the centrifuge tube 3 drips, but honeybee can suck the food in the centrifuge tube through this opening, and honeybee behaviour area 1 top opposite side offers the blow vent 4 that a usefulness net is sealed; Both reached circulation of air, and prevented the honeybee escape again, filter paper 5 is tiled in essential oil rest area 2 bottoms; Be used to load dalbergia wood essential oil, and promote volatilization.Agree with essential oil rest area 2 honeybee behaviour area 1, can freely dismantle.
Embodiment two
Take by weighing 100 gram dalbergia wood medicinal materials, " method that middle volatile oil of Chinese pharmacopoeia extracts adds 300 ml waters after pulverizing the dalbergia wood medicinal material, adopts altogether little the boiling of hydrodistillation to extract essential oil in 5 hours according to 2010 editions.20 honeybees and 10 honeybee mites are put into the honeybee behaviour area 1 of the device of embodiment one, and on the filter paper 5 of essential oil rest area 2 bottoms placement, pour 0.5 milliliter in dalbergia wood essential oil into, essential oil is volatilization naturally at room temperature, acts on honeybee and honeybee mite.
The result: have part honeybee mite to drop from honeybee in 1 hour on one's body, honeybee does not have toxic reaction.The honeybee mite is dead 9 after 48 hours, and honeybee does not have death.The dalbergia wood essential oil of 0.5 milliliter of consumption of decidable has miticidal effect preferably, and is nontoxic to honeybee simultaneously.
Embodiment three
Take by weighing 100 gram flower bud of lily magnolia medicinal materials, pulverize the back and add 400 ml waters, essential oil was extracted in employing little the boiling of hydrodistillation altogether in 5 hours.20 honeybees and 10 honeybee mites are put into the honeybee behaviour area 1 of the device of embodiment one, and pour 0.5 milliliter in the flower bud of lily magnolia essential oil of said extracted on the filter paper of placing in essential oil rest area 2 bottoms 5 into, essential oil at room temperature volatilizees, and acts on honeybee and honeybee mite.
The result: honeybee and honeybee mite all do not have toxic reaction in 1 hour.The honeybee mite is dead 1 after 48 hours, 5 of bee deaths.The flower bud of lily magnolia essential oil miticidal effect of 0.5 milliliter of consumption of decidable is relatively poor, simultaneously honeybee is had low toxicity.
Embodiment four
Take by weighing 100 gram cloves medicinal materials, pulverize the back and add 500 ml waters, essential oil was extracted in employing little the boiling of hydrodistillation altogether in 5 hours.20 honeybees and 10 honeybee mites are put into the honeybee behaviour area 1 of the device of embodiment one, and pour 0.5 milliliter of the lilac compound oil of said extracted on the filter paper of placing in essential oil rest area 2 bottoms 5 into, essential oil at room temperature volatilizees, and acts on honeybee and honeybee mite.
The result: have part honeybee mite to drop from honeybee in 1 hour on one's body, honeybee does not have toxic reaction.The honeybee mite is dead 10 after 48 hours, 5 of bee deaths.The lilac compound oil of 0.5 milliliter of consumption of decidable has good miticidal effect, but simultaneously honeybee is had low toxicity.
Embodiment five
Take by weighing 100 gram cloves medicinal materials, pulverize the back and add 500 ml waters, essential oil was extracted in employing little the boiling of hydrodistillation altogether in 5 hours.20 honeybees and 10 honeybee mites are put into the honeybee behaviour area 1 of the device of embodiment one, and splash into 0.3 milliliter of the lilac compound oil of said extracted on the filter paper of placing in essential oil rest area 2 bottoms 5, essential oil at room temperature volatilizees, and acts on honeybee and honeybee mite.
The result: have part honeybee mite to drop from honeybee in 1 hour on one's body, honeybee does not have toxic reaction.The honeybee mite is dead 10 after 48 hours, and honeybee does not have death.The lilac compound oil of 0.3 milliliter of consumption of decidable has good miticidal effect, simultaneously to the honeybee avirulence.
Embodiment six
Take by weighing 100 gram beans bandit medicinal materials, pulverize the back and add 1000 ml waters, essential oil was extracted in employing little the boiling of hydrodistillation altogether in 5 hours.20 honeybees and 10 honeybee mites are put into the honeybee behaviour area 1 of the device of embodiment one, and splash into 0.5 milliliter in the cardamom essential oil of said extracted on the filter paper of placing in essential oil rest area 2 bottoms 5, essential oil at room temperature volatilizees, and acts on honeybee and honeybee mite.The result: have part honeybee mite to drop from honeybee in 1 hour on one's body, honeybee has toxic reaction.The honeybee mite is dead 9 after 48 hours, 15 of bee deaths.The cardamom essential oil of 0.5 milliliter of consumption of decidable has good miticidal effect, but simultaneously honeybee is had higher toxicity.
Claims (3)
1. the device of a raising honeybee; It is characterized in that this device is made up of honeybee behaviour area (1) and essential oil rest area (2), honeybee behaviour area (1) and essential oil rest area (2) are separated through gauze (6); Gauze (6) is fixed on bottom, honeybee behaviour area (1); Top, honeybee behaviour area (1) one side is equipped with a centrifuge tube with cover (3), and centrifuge tube (3) bottom is an opening, and diameter is 2-3mm; Top, honeybee behaviour area (1) opposite side is provided with the blow vent (4) that a usefulness net is sealed, and filter paper (5) is tiled in essential oil rest area (2) bottom.
2. the device of a kind of raising honeybee according to claim 1 is characterized in that, agree with essential oil rest area (2) top honeybee behaviour area (1) bottom, freely dismantles.
3. the application of the device of a kind of raising honeybee according to claim 1 in the anti-mite Obtaining Perfume Essential Oil of screening.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011103954446A CN102511409B (en) | 2011-12-03 | 2011-12-03 | Device for breeding bees and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011103954446A CN102511409B (en) | 2011-12-03 | 2011-12-03 | Device for breeding bees and application |
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| CN102511409A true CN102511409A (en) | 2012-06-27 |
| CN102511409B CN102511409B (en) | 2013-11-20 |
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| CN2011103954446A Expired - Fee Related CN102511409B (en) | 2011-12-03 | 2011-12-03 | Device for breeding bees and application |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104082186A (en) * | 2014-07-28 | 2014-10-08 | 环境保护部南京环境科学研究所 | Bee acute oral toxicity experimental apparatus |
| CN104969909A (en) * | 2015-07-08 | 2015-10-14 | 中国农业科学院蜜蜂研究所 | Method for researching bee feeding behaviors and device |
| CN107389870A (en) * | 2017-08-10 | 2017-11-24 | 云南农业大学 | It is a kind of that stomach toxicity and toxicity test device and method of tagging are carried out to Frankliniella occidentalis |
| CN108450368A (en) * | 2018-03-30 | 2018-08-28 | 高晓歌 | A kind of device for bee variety mite sneak case of quarantining |
| CN108541675A (en) * | 2018-06-27 | 2018-09-18 | 中国农业科学院蜜蜂研究所 | A kind of harvester of living body bee sample |
| CN111109156A (en) * | 2020-01-23 | 2020-05-08 | 云南省农业科学院蚕桑蜜蜂研究所 | Large-scale bee feeding device for scientific research and bee feeding method thereof |
| CN114304070A (en) * | 2021-12-13 | 2022-04-12 | 中国农业科学院蜜蜂研究所 | Method and device for detecting bee mite infection rate |
| CN118091105A (en) * | 2024-04-27 | 2024-05-28 | 山东亿嘉农化有限公司 | Analysis method and analysis device for toxicity of pesticide to bees |
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| WO2003037076A1 (en) * | 2001-10-30 | 2003-05-08 | Urpo Vainio Oy | Method and device for destroying acarids |
| CN2715540Y (en) * | 2004-06-17 | 2005-08-10 | 叶达华 | Medicinal tablet for killing bees and mites |
| CN101278681A (en) * | 2008-05-14 | 2008-10-08 | 陈盛禄 | Prescription for preventing and controlling bee mite |
| CN101703008A (en) * | 2009-11-05 | 2010-05-12 | 浙江大学 | Anti-mite breeding beehive |
-
2011
- 2011-12-03 CN CN2011103954446A patent/CN102511409B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2003037076A1 (en) * | 2001-10-30 | 2003-05-08 | Urpo Vainio Oy | Method and device for destroying acarids |
| CN2715540Y (en) * | 2004-06-17 | 2005-08-10 | 叶达华 | Medicinal tablet for killing bees and mites |
| CN101278681A (en) * | 2008-05-14 | 2008-10-08 | 陈盛禄 | Prescription for preventing and controlling bee mite |
| CN101703008A (en) * | 2009-11-05 | 2010-05-12 | 浙江大学 | Anti-mite breeding beehive |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104082186A (en) * | 2014-07-28 | 2014-10-08 | 环境保护部南京环境科学研究所 | Bee acute oral toxicity experimental apparatus |
| CN104969909A (en) * | 2015-07-08 | 2015-10-14 | 中国农业科学院蜜蜂研究所 | Method for researching bee feeding behaviors and device |
| CN107389870A (en) * | 2017-08-10 | 2017-11-24 | 云南农业大学 | It is a kind of that stomach toxicity and toxicity test device and method of tagging are carried out to Frankliniella occidentalis |
| CN108450368A (en) * | 2018-03-30 | 2018-08-28 | 高晓歌 | A kind of device for bee variety mite sneak case of quarantining |
| CN108541675A (en) * | 2018-06-27 | 2018-09-18 | 中国农业科学院蜜蜂研究所 | A kind of harvester of living body bee sample |
| CN111109156A (en) * | 2020-01-23 | 2020-05-08 | 云南省农业科学院蚕桑蜜蜂研究所 | Large-scale bee feeding device for scientific research and bee feeding method thereof |
| CN114304070A (en) * | 2021-12-13 | 2022-04-12 | 中国农业科学院蜜蜂研究所 | Method and device for detecting bee mite infection rate |
| CN118091105A (en) * | 2024-04-27 | 2024-05-28 | 山东亿嘉农化有限公司 | Analysis method and analysis device for toxicity of pesticide to bees |
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| Publication number | Publication date |
|---|---|
| CN102511409B (en) | 2013-11-20 |
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