CN205620290U - Micro -fluidic detection device and system - Google Patents
Micro -fluidic detection device and system Download PDFInfo
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- CN205620290U CN205620290U CN201620401052.4U CN201620401052U CN205620290U CN 205620290 U CN205620290 U CN 205620290U CN 201620401052 U CN201620401052 U CN 201620401052U CN 205620290 U CN205620290 U CN 205620290U
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- 238000001514 detection method Methods 0.000 title claims abstract description 54
- 239000007788 liquid Substances 0.000 claims abstract description 64
- 239000002699 waste material Substances 0.000 claims abstract description 15
- 238000007405 data analysis Methods 0.000 claims description 7
- 238000002347 injection Methods 0.000 claims description 7
- 239000007924 injection Substances 0.000 claims description 7
- 238000000034 method Methods 0.000 claims description 6
- 238000004458 analytical method Methods 0.000 claims description 5
- 239000000919 ceramic Substances 0.000 claims description 4
- 230000000694 effects Effects 0.000 claims description 4
- 238000006243 chemical reaction Methods 0.000 description 6
- 238000010586 diagram Methods 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 5
- 239000012530 fluid Substances 0.000 description 4
- 239000004743 Polypropylene Substances 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 239000012085 test solution Substances 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000009795 derivation Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- -1 polypropylene Polymers 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- 238000012031 short term test Methods 0.000 description 1
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Abstract
The utility model discloses a micro -fluidic detection device, include: base, application of sample device and negative pressure pump, the application of sample unit bit wherein, has chip slot and waste liquid groove in the base top on the base, the application of sample device includes rotating circular disk, cylinder and liquid storage pipe, wherein rotating circular disk and cylinder fixed connection, have a plurality of fixed positions on the rotating circular disk, the liquid storage pipe is placed in fixed position during the use, examine time measuring, wait that the micro -fluidic chip who detects places in chip slot, adjust the height of rotating circular disk through the cylinder to the control rotating circular disk is rotatory, so that the liquid storage pipe is located the top of micro -fluidic chip's notes liquid mouth, micro -fluidic chip's liquid outlet and negative pressure pump are connected, and the negative pressure pump is arranged in pouring into the waste liquid into the waste liquid groove. The utility model also discloses a micro -fluidic detecting system. Utilize the utility model discloses can realize high -efficient quick micro -fluidic detection.
Description
Technical Field
The utility model relates to a micro-fluidic chip technique, concretely relates to micro-fluidic detection device and system.
Background
The microfluidic technology is a newly developed technology in recent years, mainly uses analytical chemistry, controls fluid in a micron-sized structure, performs reaction detection, and has the advantages of small volume, less reagent and sample consumption, capability of simultaneously detecting multiple samples and multiple indexes and the like. In the past, the detection needs a sample amount of a few milliliters, only needs a sample amount of a few microliters after the micro-fluidic chip technology is adopted, so that the consumption of samples and reagents is greatly saved, the performance index is superior to that of a common detection method, and the method has wide application prospect and important application value.
The microfluidic chip is a main platform for realizing the microfluidic technology and is mainly characterized in that various functions of a chip system are completed by controlling the flow of fluid in a microfluidic channel. At present, the drive and control of microfluid in a microfluidic chip system are realized by matching pumps (or externally connected pumps) integrated in various forms in a chip with valve bodies in various forms, so that most of the existing microfluidic analysis and detection instruments are in a manual and semi-automatic state, and the instruments are expensive, large in size and inconvenient to operate. Throughout the field of microfluidic detection, some existing microfluidic detection devices are simple in structure and single in fluid control function, and some existing microfluidic detection devices can realize control of various forms of microfluid, but are complex in structure, difficult to process and high in cost, and are not beneficial to mass production. Moreover, most of the existing microfluidic detection devices need to be equipped with various microfluidic control facilities with large volume because the valve body in the chip has unsatisfactory functions, which obviously does not meet the requirements of miniaturization and portability of the microfluidic chip technology and cannot meet the requirements of the microfluidic chip on processing complex fluids.
Disclosure of Invention
In view of this, the utility model provides a micro-fluidic detection device and system can realize high-efficient quick micro-fluidic detection.
In one aspect, the utility model provides a micro-fluidic detection device, include: the device comprises a base, a sample adding device and a negative pressure pump, wherein the sample adding device is positioned above the base, and the base is provided with a chip groove and a waste liquid groove; the sample adding device comprises a rotating disc, an air cylinder and a liquid storage pipe, wherein the rotating disc is fixedly connected with the air cylinder; the rotating disc is provided with a plurality of fixed positions, and the liquid storage pipe is placed in the fixed positions when the rotating disc is used; during detection, a micro-fluidic chip to be detected is placed in the chip groove, the height of the rotating disc is adjusted through the air cylinder, the rotating disc is controlled to rotate, so that the liquid storage pipe is located above a liquid injection port of the micro-fluidic chip, a liquid outlet of the micro-fluidic chip is connected with a negative pressure pump, and the negative pressure pump is used for injecting waste liquid into the waste liquid groove.
Preferably, the tip of the liquid storage tube is provided with piezoelectric ceramics for controlling the flow of liquid in the liquid storage tube through piezoelectric effect.
Preferably, the rotating disk is connected with a zero point positioning system, and the rotation and the positioning of the rotating disk are controlled by the zero point positioning system.
Preferably, the chip groove has a size corresponding to the size of the microfluidic chip to be detected.
Preferably, the micro-fluidic chip to be detected is a micro-fluidic chip of an S-shaped pipeline.
On the other hand, the utility model also provides a micro-fluidic detection system, which comprises a data acquisition module, a data analysis module and the micro-fluidic detection device; wherein,
the data acquisition module is used for acquiring detection signals in the detection process of the microfluidic detection device;
the data analysis module is used for carrying out preset analysis processing on the detection signals acquired by the data acquisition module.
Preferably, the data acquisition module is a CCD camera.
Preferably, the data analysis module is a computer.
The embodiment of the utility model provides a micro-fluidic detection device simple structure, processing convenience, low cost, the easy industrialization production that realizes. Compared with the prior art, the utility model discloses micro-fluidic detection device and system simple operation possess a great deal of advantages such as miniaturation, automation, portability, high-efficient short-term test.
Drawings
Fig. 1 is a schematic structural diagram of a microfluidic detection device according to a preferred embodiment of the present invention.
Fig. 2 is a schematic structural diagram of a base according to an embodiment of the present invention.
Fig. 3 is a schematic structural diagram of a sample adding device according to an embodiment of the present invention.
Fig. 4 is a schematic structural diagram of a microfluidic chip to be detected according to an embodiment of the present invention.
Detailed Description
The technical solution of the present invention will be described in detail below with reference to the accompanying drawings and specific embodiments.
The utility model discloses micro-fluidic detection device includes: the device comprises a base, a sample adding device and a negative pressure pump, wherein the sample adding device is positioned above the base, and the base is provided with a chip groove and a waste liquid groove; the sample adding device comprises a rotating disc, an air cylinder and a liquid storage pipe, wherein the rotating disc is fixedly connected with the air cylinder; the rotating disc is provided with a plurality of fixing positions, and the liquid storage pipe is placed in the fixing positions during use. During detection, a micro-fluidic chip to be detected is placed in the chip groove, the height of the rotating disc is adjusted through the air cylinder, the rotating disc is controlled to rotate, so that the liquid storage pipe is located above a liquid injection port of the micro-fluidic chip, a liquid outlet of the micro-fluidic chip is connected with a negative pressure pump, and the negative pressure pump is used for injecting waste liquid into the waste liquid groove.
In the actual operation, a micro-fluidic chip to be detected is placed in a chip groove of a base, a negative pressure pump is connected, the number of liquid storage tubes is determined according to the requirement of a detection program, a corresponding test solution is added into the liquid storage tubes, the liquid storage tubes are fixed on a rotating disc, the rotating disc is controlled to move up and down by using an air cylinder, the rotating disc is controlled to rotate and position manually or by using a motor, so that the outlet of the liquid storage tubes is aligned to a liquid injection port of the micro-fluidic chip, then the test solution is controlled to flow out and be injected into the micro-fluidic chip, the negative pressure pump is started after the reaction, and waste liquid is injected into; and then the rotating disc is rotated, and the liquid in the next liquid storage tube is injected into the microfluidic chip, and the steps are sequentially carried out until all reaction processes are finished.
Fig. 1 shows a schematic structural diagram of a microfluidic detection device according to a preferred embodiment of the present invention, wherein a microfluidic chip is placed in a chip slot 1-1 of a base 1, a sample adding device 2 is disposed above the base 1, a rotating disc 2-1 is connected to an upper portion of a cylinder 2-2, a plurality of fixing positions are arranged along a circumferential direction of the rotating disc 2-1, and a liquid storage tube 2-3 is placed in the fixing positions.
Fig. 2 shows the structure of the base of the embodiment of the present invention, wherein the size of the chip groove 1-1 should be adapted to the size of the micro-fluidic chip to be detected. Aiming at different detection tasks, the micro-fluidic chip to be detected can be any micro-fluidic chip, such as a micro-fluidic chip of an S-shaped pipeline, and the micro-fluidic chip to be detected is provided with a liquid injection port and a liquid outlet.
Fig. 3 shows the structure of the sample adding device according to the embodiment of the present invention, wherein the rotating disc 2-1 is connected to the upper portion of the cylinder 2-2, and a plurality of fixing positions are arranged along the circumferential direction of the rotating disc 2-1, and the detachable liquid storage tube 2-3 is placed in the fixing positions. The material of the liquid storage tube 2-3 is selected from high molecular material, such as polypropylene PP.
In the embodiment of the present invention, piezoelectric ceramics (not shown in the figure) are placed at the tip of the liquid storage tube 2-3, and the flow of liquid in the liquid storage tube is controlled by piezoelectric effect during detection.
In the embodiment of the present invention, the rotating disc 2-1 is connected to a zero positioning system (not shown in the figure), and the rotation and positioning of the rotating disc are controlled by the zero positioning system during the detection.
Fig. 4 shows the structure of the micro-fluidic chip to be detected, which has a liquid injection port 3 and a liquid outlet port 4.
When the microfluidic detection device provided by the embodiment of the utility model is used for detection, a microfluidic chip is placed in the chip groove 1-1, the liquid injection port 3 of the chip is arranged below the rotating disc 2-1, and the cylinder 2-2 can drive the rotating disc 2-1 to move up and down; the rotary disc 2-1 is fixedly provided with detachable liquid storage tubes 2-3, and the number of the liquid storage tubes 2-3 can be adjusted according to a detection program; a zero positioning system is adopted to control the rotation of the rotating disc 2-1, so that the liquid storage pipe 2-3 is controlled to correspond to the detection step; piezoelectric ceramics are placed at the tip end of the liquid storage tube 2-3, and the flow of liquid in the liquid storage tube 2-3 is controlled through the piezoelectric effect to complete the whole reaction process. A hydraulic pump is connected to the liquid outlet 4, and the waste liquid after reaction is pumped into the waste liquid tank 1-2.
Further, based on the microfluidic detection device of the above embodiment, a data acquisition module and a data analysis module may be configured for the device, and are used for acquiring detection signals and performing result analysis in the detection process of the microfluidic detection device. Specifically, a reaction signal, such as a chemiluminescence signal, of the microfluidic chip in the chip slot 1-1 can be photographed by a CCD camera, and then a picture photographed by the CCD camera is transmitted to a computer, and is analyzed and processed by using relevant software, so as to obtain a detection analysis result.
The technical solution of the present invention is described in detail with reference to the specific embodiments, and the described specific embodiments are used to help understand the idea of the present invention. The derivation and modification made by the person skilled in the art on the basis of the specific embodiments of the present invention also belong to the protection scope of the present invention.
Claims (8)
1. A microfluidic detection device, comprising: a base, a sample adding device and a negative pressure pump, wherein the sample adding device is positioned above the base,
the base is provided with a chip groove and a waste liquid groove;
the sample adding device comprises a rotating disc, an air cylinder and a liquid storage pipe, wherein the rotating disc is fixedly connected with the air cylinder; the rotating disc is provided with a plurality of fixed positions, and the liquid storage pipe is placed in the fixed positions when the rotating disc is used;
during detection, a micro-fluidic chip to be detected is placed in the chip groove, the height of the rotating disc is adjusted through the air cylinder, the rotating disc is controlled to rotate, so that the liquid storage pipe is located above a liquid injection port of the micro-fluidic chip, a liquid outlet of the micro-fluidic chip is connected with a negative pressure pump, and the negative pressure pump is used for injecting waste liquid into the waste liquid groove.
2. The microfluidic detection device according to claim 1, wherein the tip of the reservoir tube is placed with a piezoelectric ceramic for controlling the flow of liquid in the reservoir tube by piezoelectric effect.
3. The microfluidic detection device according to claim 1 or 2, wherein the rotating disk is connected with a zero positioning system, and the rotation and positioning of the rotating disk are controlled by the zero positioning system.
4. The microfluidic detection device according to claim 1 or 2, wherein the chip slot has a size corresponding to the size of the microfluidic chip to be detected.
5. The microfluidic detection device according to claim 1 or 2, wherein the microfluidic chip to be detected is an S-channel microfluidic chip.
6. A microfluidic detection system comprising a data acquisition module, a data analysis module, and a microfluidic detection device according to any one of claims 1-5; wherein,
the data acquisition module is used for acquiring detection signals in the detection process of the microfluidic detection device;
the data analysis module is used for carrying out preset analysis processing on the detection signals acquired by the data acquisition module.
7. The microfluidic detection system of claim 6, wherein the data acquisition module is a CCD camera.
8. The microfluidic detection system of claim 6 or 7, wherein the data analysis module is a computer.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620401052.4U CN205620290U (en) | 2016-05-05 | 2016-05-05 | Micro -fluidic detection device and system |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201620401052.4U CN205620290U (en) | 2016-05-05 | 2016-05-05 | Micro -fluidic detection device and system |
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| CN205620290U true CN205620290U (en) | 2016-10-05 |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105823727A (en) * | 2016-05-05 | 2016-08-03 | 北京纳迅科技股份有限公司 | Microfluid control detecting device and system |
| CN107308997A (en) * | 2017-06-22 | 2017-11-03 | 浙江诺迦生物科技有限公司 | A kind of micro-fluidic chip convenient type Ngatively pressurized sampling device based on miniature suction ball |
| CN110586220A (en) * | 2019-10-16 | 2019-12-20 | 陕西优博特生物科技有限公司 | Liquid feeding and sample feeding device for micro-fluidic chip |
| WO2020034143A1 (en) * | 2018-08-16 | 2020-02-20 | 深圳华大智造科技有限公司 | Handle device, positioning device, loading device and gene sequencer |
| CN113125693A (en) * | 2021-03-08 | 2021-07-16 | 中山大学 | Small-sized portable full-automatic enzyme-linked immunoassay analyzer and application thereof |
-
2016
- 2016-05-05 CN CN201620401052.4U patent/CN205620290U/en active Active
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105823727A (en) * | 2016-05-05 | 2016-08-03 | 北京纳迅科技股份有限公司 | Microfluid control detecting device and system |
| CN105823727B (en) * | 2016-05-05 | 2018-10-23 | 北京纳迅科技股份有限公司 | A kind of micro-fluidic detection device and system |
| CN107308997A (en) * | 2017-06-22 | 2017-11-03 | 浙江诺迦生物科技有限公司 | A kind of micro-fluidic chip convenient type Ngatively pressurized sampling device based on miniature suction ball |
| WO2020034143A1 (en) * | 2018-08-16 | 2020-02-20 | 深圳华大智造科技有限公司 | Handle device, positioning device, loading device and gene sequencer |
| CN110586220A (en) * | 2019-10-16 | 2019-12-20 | 陕西优博特生物科技有限公司 | Liquid feeding and sample feeding device for micro-fluidic chip |
| CN110586220B (en) * | 2019-10-16 | 2023-07-04 | 河南优博特生物科技有限公司 | Liquid feeding and sampling device for microfluidic chip |
| CN113125693A (en) * | 2021-03-08 | 2021-07-16 | 中山大学 | Small-sized portable full-automatic enzyme-linked immunoassay analyzer and application thereof |
| CN113125693B (en) * | 2021-03-08 | 2022-09-16 | 中山大学 | Small-sized portable full-automatic enzyme-linked immunoassay analyzer and application thereof |
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